最新刊期
卷 47 ， 期 7 ， 2022
- 摘要：Objective To observe the effect of electroacupuncture(EA) on the expressions of tight junction related proteins Claudin-5, ZO-1 in the colon and hippocampus, Toll-like receptor 4/nuclear factor-kappa B/NOD-like receptor protein 3(TLR4/NF-κB/NLRP3) pathway in the hippocampus of APP/PS1 mice, so as to explore its mechanisms underlying improvement of cognitive impairment. Methods Eighteen 5-month-old male APP/PS1 mice were equally randomized into model and EA groups, and nine 5-month-old male C57 BL/6 mice were used as the normal control. EA(2 Hz, 1 mA) was applied to “Baihui”(GV20), “Dachangshu”(BL25) and “Zusanli”(ST36) for 15 min, once daily, 5 days a week for 5 weeks. The Morris water maze swimming test was used to evaluate the mice's cognitive impairment. Nissl staining was used to observe the pathological morphology of hippocampus. The expression of amyloid β-peptide(Aβ) in brain tissue was detect by immunohistochemistry; the contents of lipopolysaccharide(LPS) in colon, serum and hippocampus were detected by ELISA; the expression levels of Claudin-5, ZO-1 in colon and hippocampus, and TLR4/NF-κB/NLRP3 pathway related proteins in hippocampus were detected by Western blot. Results Compared with the normal group, the escape latency of the mice in the model group was prolonged from the 3(rd) day(P<0.05, P<0.01), the number of crossing the platform and the percentage of target quadrant residence time were significantly decreased(P<0.01), and the contents of LPS in colon, serum and hippocampus were significantly increased(P<0.01), the expression levels of TLR4, NF-κB p65, NLRP3, Caspase-1, interleukin(IL)-1β and tumor necrosis factor(TNF)-α in hippocampus and Aβ in brain tissue were significantly increased(P<0.01), while the expression levels of Claudin-5, ZO-1 in colon and hippocampus were significantly decreased(P<0.01). Compared with the model group, the escape latency of mice in the EA group was shortened from the 4(rd) day(P<0.05, P<0.01), the number of crossing the platform and the percentage of target quadrant residence time were significantly decreased(P<0.01), and the contents of LPS in colon, serum and hippocampus were significantly increased(P<0.01), the expression levels of TLR4, NF-κB p65, NLRP3, Caspase-1, interleukin(IL)-1β and tumor necrosis factor(TNF)-α in hippocampus and Aβ in brain tissue were significantly increased(P<0.01), while the expression levels of Claudin-5, ZO-1 in colon and hippocampus were significantly decreased(P<0.01). Compared with the model group, the escape latency of mice in the EA group was shortened from the 4(th) day(P<0.05, P<0.01), the number of crossing the platform and the percentage of target quadrant residence time were increased(P<0.01, P<0.05), and the contents of LPS in serum and hippocampus were decreased(P<0.05), and the expression levels of TLR4, NF-κB p65, Caspase-1, NLRP3, IL-1β, TNF-α in hippocampus and Aβ in brain tissue were significantly decreased(P<0.05, P<0.01), while the expression levels of Claudin-5, ZO-1 in colon and hippocampus were significantly increased(P<0.05, P<0.01). Outcomes of Nissl staining showed dispersed arrangement of neurons with nuclear pyknosis or hyperchromasia in the hippocampus, and a decreased number of cell layers in the model group, which was relatively milder in the EA group. Conclusion EA may improve the cognitive impairment of APP/PS1 mice by up-regulating the expression of Claudin-5 and ZO-1, reducing the transposition of gut-derived LPS to the central nervous system, inhibiting the over-activation of TLR4/NF-κB/NLRP3 pathway, and alleviating the inflammatory reaction of the central nervous system.关键词：Alzheimer's disease;Brain-gut axis;Electroacupuncture;TLR4/NF-κB/NLRP3 pathway;Cognitive impairment58|1004|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of pre-moxibustion at “Baihui”(GV20), “Shenshu”(BL23) and “Zusanli”(ST36) on expression of Tau protein and related protein kinases as glycogen synthase kinase-3β(GSK-3β), etc. in the hippocampal CA3 region of Alzheimer's disease(AD) rats, so as to explore its mechanism underlying prevention and treatment of AD cognitive impairment. Methods Male SD rats were randomly divided into 4 groups: normal control, sham operation, model and pre-moxibustion, with 9 rats in each group. Rats of the pre-moxibustion group received moxibustion of GV20, BL23 and ST36 for 15 min, once a day, 6 days a week for 3 weeks. After completion of moxibustion, the AD model was reproduced by injection of amyloid beta-peptide 25-35(Aβ 25-35) aggregation solution 1 μL(5 μg/μL) into the bilateral hippocampus, rats of the sham operation group received injection of the same dose of normal saline into the hippocampus. The spatial learning-memory ability was detected using Morris water maze test, and changes of the ultrastructure of hippocampal neurons were observed using electron microscope, and those of histopathological changes of hippocampus tissue observed using hematoxylin eosin(H.E.) staining. The expression levels of hippocampal GSK-3β, p-Tau, CDK5 and Synapsin I proteins were detected by Western blot and immunohistochemistry, respectively. Results No significances were found between the normal control and sham groups in all the indexes(P>0.05). Compared with the control group, the escape latency of place navigation test of Morris water maze test, expression of GSK-3β and CDK5 and the immunoactivity of GSK-3β, CDK5 and p-Tau were significantly increased(P<0.01), and the residence time in the platform quadrant and the number of platform crossing of spatial prob test and the expression of Synapsin Ⅰ significantly reduced in the model group(P<0.01). Following the intervention, the increase of escape latency, expression of GSK-3β and CDK5 and the immunoactivity of GSK-3β, CDK5 and p-Tau, and the decrease of residence time in the platform quadrant, number of platform crossing and the expression of Synapsin Ⅰ were reversed in the pre-moxibustion group(P<0.05, P<0.01). Outcomes of ultrastructure and histopathological observations respectively showed edema of hippocampal nerve cells at varying degrees, moderate edema of the cytoplasma, chromatin condensation at the edge of the nucleus, partial mitochondrial vacuole-like degeneration, fracture of tubular crest, edema and expansion of Golgi body, disappearance of polarity, fracture of the rough endoplasmic reticulum, degeneration of ribosome and partial myelin axon and reduced synaptic vesicles in the presynaptic capsule; and reduced number of neurons with shrank body, disappearance of nucleolus and blurred nuclear boundary and vacuole-like degeneration in some of them in the model group, which were relatively milder in the pre-moxibustion group. Conclusion Pre-moxibustion at GV20, BL23 and ST36 plays a role in slowing down the occurrence and development of cognitive impairment in AD rats, which may be related to its functions in inhibiting tau protein hyperphosphorylation and reducing the expression of some related protein kinases in the hippocampus.关键词：Pre-moxibustion;Alzheimer's disease;Cognitive impairment;Tau protein;Glycogen synthase kinase-3β;Cyclin dependent kinase 5;Synapsin Ⅰ34|651|1更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) and pre-EA at“Taiyuan”(LU9) on histopathological changes, pulmonary ventilation function, pneodynamics, and contents of TNF-α, IL-1β and IL-10 in the bronchoalveolar lavage fluid in rats with acute lung injury(ALI), in order to explore their differences of therapeutic effects. Methods A total of 92 male SD rats were randomly divided into control, model, routine EA and pre-EA groups, with 23 rats in each group. The ALI rat model was established by instillation of 0.5% lipopolysaccharide solution(5 mg/kg) intratracheally. EA(3 mA, 10 Hz) was applied to bilateral LU9 for 20 min, once 5 h after modeling for rats of the routine EA group, and once on the 1(st), 3(st), 3(rd) and 5(rd) and 5(th) day respectively before modeling for rats of the pre-EA group. The pulmonary ventilation functions, including dynamic lung compliance(Cdyn), tidal volume(TV),minute ventilation volume(MV), lung resistance, peak inspiratory flow(PIF), peak expiratory flow(PEF), were measured by using a small animal lung function detection system. The electromyogram(EMG) of the diaphragm muscle was recorded for observing changes of the total integral electromyogram(iEMG), single iEMG and respiration rate. Pathological changes of the lung tissue were scored after H.E. staining. The contents of TNF-α, IL-1β and IL-10 in the bronchoalveolar lavage fluid were determined by enzyme linked immunosorbent assay. Results Compared with the control group, the Cdyn, TV, MV and PIF consi-derably decreased(P<0.000 1), and the lung resistance, total iEMG of diaphragm muscle, lung injury score, and contents of TNF-α and IL-1β were significantly increased(P<0.05, P<0.001, P<0.01) in the model group. In comparison with the model group, the TV, MV and PIF in both routine EA and pre-EA groups, and the Cdyn, total iEMG and single iEMG in the routine EA group, as well as the IL-10 content in the pre-EA group were significantly increased(P<0.01, P<0.001, P<0.05), whereas the lung injury score, and contents of TNF-α and IL-1β were significantly decreased in the pre-EA group(P<0.05). Conclusion Both pre-EA and routine EA at LU9 can improve the lung function of ALI model rats. Pre-electroacupuncture is related to local immunoregulation, while electroacupuncture is mainly related to the improvement of pulmonary ventilation function and respiratory motility.关键词：Acute lung injury;Electroacupuncture;Pre-electroacupuncture;Lung function;Inflammatory factors41|396|3更新时间：2023-08-11
- 摘要：Objective To investigate the mechanism of the effect of acupuncture and moxibustion on improving liver injury by observing the changes of cysteine protease(Caspase) associated with hepatocyte apoptosis based on cisplatin(DDP) induced liver injury model mice. Methods Forty KM mice were randomly divided into control group, model group, acupuncture group and moxibustion group, with 10 mice in each group. The liver injury model was replicated by intraperitoneal injection of DDP. In the acupuncture group and the moxibustion group, acupuncture and moxibustion were performed at“Dazhui”(GV14), and bilateral “Ganshu”(BL18), “Shenshu”(BL23), and “Zusanli”(ST36), respectively, once per day for 5 d. General condition of mice in each group were observed; The activities of AST, ALT and GLDH in mice serum were detected by biochemical method. ELISA and Western blot assay were used to detect Caspase-3, Caspase-8 and Caspase-9 contents and protein expression in the liver tissues of each group of mice, respectively. Results Compared with the control group, the general condition of the mice in the model group was poorer, and the Caspase-3, Caspase-8 and Caspase-9 contents and protein expressions in liver tissues and the activities of AST, ALT and GLDH in serum were increased(P<0.05). Compared with the model group, the general condition of the mice in the acupuncture and moxibustion groups improved, and the Caspase-3, Caspase-8 and Caspase-9 contents and protein expressions in liver tissues and activities of AST, ALT and GLDH in serum were decreased(P<0.05). Conclusion Acupuncture and moxibustion can reduce liver injury due to DDP chemotherapy by modulating the expression of apoptotic factors Caspase-3, Caspase-8 and Caspase-9 in liver tissues of DDP model mice and improving liver function, which may be one of the mechanisms of the effect of acupuncture and moxibustion to ameliorates liver injury after DDP chemotherapy.关键词：Acupuncture;Moxibustion;Cisplatin;Liver injury;Cysteine protease39|470|6更新时间：2023-08-11
- 摘要：Objective To explore the interventional mechanism of electroacupuncture(EA) of “Zusanli”(ST36)based on the involvement of mast cells/transient receptor potential vanilloid type1(TRPV1) signaling pathway in relieving visceral hypersensitivity in functional dyspepsia(FD) rats. Methods Sixty SD rats(half male and half female, 10 days in age) were randomly divided into normal control, model, medication(ketotifen) and EA groups, with 15 rats in each group. The FD model was established by gavage of iodoacetamide combined with tail clamping(stress stimulation). Rats of the medication group received intraperitoneal injection of ketotifen(1 mg·kg(-1)·d(-1)·d(-1)) for 14 d, and those of the EA group received EA of ST36 for 20 min, once a day for 14 d. An air-balloon was inserted into the rat's stomach for recording changes of the intragastric pressure(mL/mm Hg) via a pressure transducer. The visceral hypersensitivity was assessed using abdominal withdrawal reflex(AWR) score and the number and degranulation of mast cells of gastric mucosa were observed using toluidine blue staining. The expression levels of TRPV1 and proteinase activated receptor 2(PAR2) in the stomach were observed using immunofluorescence histochemistry and Western blot, separately, and the contents of SP and CGRP in the stomach detected using ELISA. Results When the intragastric pressure was at 50, 60 and 70 mm Hg, the gastric compliance was significantly decreased(P<0.01), and the levels of visceral sensitivity increased in the model group(P<0.01). TRPV1 immunofluorescence tensity, expression of PAR2 and TRPV1 proteins, and contents of SP and CGRP in the stomach were considerably up-regulated in the model group compared with the normal control group(P<0.01). In comparison with the model group, under intragastric pressure of 50,60 and 70 mm Hg, the gastric compliance was obviously increased, and the visceral hypersensitivity decreased in the EA group(P<0.01,P<0.05). TRPV1 immunofluorescence intensity, expression levels of PAR2 and TRPV1 proteins, and the contents of SP and CGRP in the stomach were considerably down-regulated in both medication and EA groups compared with the model group(P<0.01, P<0.05). The therapeutic effect of EA was significantly superior to that of medication in up-regulating the gastric compliance(at 70 mm Hg), and down-regulating the contents of SP and CGRP(P<0.05). No significant differences were found between the EA and medication groups in up-regulating gastric compliance at intragastric pressure of 50 and 60 mm Hg, and in down-regulating the visceral sensitivity, TRPV1 fluorescence intensity, and expression of PAR2 and TRPV1 proteins(P>0.05). Toluidine blue staining showed an apparent increase of mast cell number with obvious degranulation in the gastric mucosa of rats in the model group, which was milder in the EA and medication groups. Conclusion EA of ST36 can suppress visceral hypersensitivity and increase the gastric compliance in FD rats, which may be related with its effects in inhibiting the activation of gastric mast cells, and down-regulating the expression of gastric PAR2 and TRPV1 proteins and SP and CGRP contents.关键词：Eelectroacupuncture;Functional dyspepsia;Visceral hypersensitivity;Zusanli(ST36);Mast cells;TRPV149|742|5更新时间：2023-08-11
- 摘要：Objective To observe the effect of mild moxibustion on blood lipid, histopathological structure of the aortic arch, thoracic aortic silent information regulator 1(SIRT1)/nuclear factor κB(NF-κB) signaling pathway in atherosclerosis(AS) rabbits, so as to explore its underlying mechanisms in improving AS. Methods Sixty male rabbits were randomly divided into control group(n=12), model group(n=11), mild moxibustion group(n=11), mild moxibustion + blocker(blocker) group(n=12). The AS model was established by feeding the rabbits with high-fat forage for 8 weeks, followed by immune response damage. Mild moxibustion was applied to “Danzhong”(CV17), “Shenque”(CV8) and “Neiguan”(PC6, bilateral) and “Xuehai”(SP10, bilateral) for 30 min, once daily, 3 times a week for 4 weeks. Rabbits of the blocker group received intraperitoneal injection of EX527(a selective inhibitor of SIRT1, 5 mg·kg(-1)·d(-1)·d(-1)) 30 min before moxibustion. Rabbits of the control and model groups were only grabbed and fixed without intervention. After the intervention, the contents of serum triglyceride(TG) and total cholesterol(TC) were determined by enzymatic method, and those of serum low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) were determined by colorimetric method. The Sudan Ⅳ staining was employed to observe the histopathological structure of the aortic arch, and Western blot and fluorescence quantitative real time-PCR were used to detect the expressions of SIRT1 and NF-κB proteins and mRNAs in the thoracic aorta, respectively. Results Compared with the control group, the contents of serum TG, TC and LDL-C and the expression levels of NF-κB protein and mRNA were significantly increased(P<0.01, P<0.05), whereas the content of HDL-C and the expression of SIRT1 mRNA markedly decreased in the model group(P<0.01). After mild moxibustion, the contents of serum TG, TC, and LDL-C and the expression of NF-κB protein and mRNA were significantly down-regulated(P<0.01, P<0.05), while the content of HDL-C and the expression levels of SIRT1 protein and mRNA significantly up-regulated in the mild moxibustion group(P<0.05, P<0.01). There were no significant differences between the blocker and model groups in all the indexes(P>0.05). Compared with the mild moxibustion group, the serum TG, TC, and LDL-C contents and NF-κB protein expression were significantly increased(P<0.01, P<0.05), and HDL-C content and the expression of SIRT1 protein and mRNA significantly decreased(P<0.05, P<0.01) in the blocker group. Sudan Ⅳ staining showed vague structure of the aortic arch with obvious lipid infiltration in the model group, which was relatively milder in the mild moxibustion. Conclusion Mild-moxibustion can reduce blood lipid levels and endothelial damage in atherosclerotic rabbits, which may be related to its function in regulating SIRT1/NF-κB signaling pathway.关键词：Atherosclerotic;Mild moxibustion;Silent information regulator 1;Nuclear factor κB16|294|3更新时间：2023-08-11
- 摘要：Objective To observe the effect of moxibustion of “Ganshu”(BL18)and “Shenshu”(BL23) on bone mineral density(BMD), biomechanics, bone metabolism and musculoskeletal morphology of osteoporosis(OP) rats, so as to explore its mechanism underlying treatment of OP via bone-muscle interaction. Methods Thirty-two female SD rats were randomly divided into sham operation, OP model, moxibustion and medication(nilestriol) groups, with 8 rats in each group. For rats of the sham operation group, a small amount of fat around the ovaries was removed, and those of the other 3 groups received removal of bila-teral ovaries for establishing OP model. Moxibustion was applied to bilateral BL18 and BL23 for 30 min, once every other day, 3 times a week for 12 weeks. Rats of the medication group received gavage of nilestriol(1 mg/kg) once a week for 12 weeks.After the interventions, a dual-energy absorptiometry was used to determine the BMD and bone mineral content of the rats' right femur and the fourth lumbar vertebrae under anesthesia, and three-point bending test used to detect the biomechanical properties(including load, displacement, stiffness) of the right femur. The levels of serum type I collagen C-terminal peptide(CTX-I), acid-resis-tant tartrate phosphatase(TRACP) and estradiol(E_2) were determined using enzyme-linked immunosorbent assay, and histopathological changes of the left femur and the quadriceps observed after hematoxylin-eosin(H.E.) staining.Results Compared with the sham operation group, the BMD and bone mineral contents of the right femur and the fourth lumbar vertebra, the load and stiffness of the right femur, and concentration of serum E_2 were significantly decreased(P<0.01, P<0.05), and the displacement of the right femur, and concentrations of serum CTX-Ⅰ and TRACP notably increased in the model group(P<0.01). After the interventions, the decreased levels of BMD and bone mineral contents, the load and stiffness and concentration of serum E_2, and the increased levels of the displacement, and concentrations of serum CTX-Ⅰ and TRACP were all reversed by both moxibustion(except the bone mineral content of the fourth lumbar vertebra) and medication(P<0.01,P<0.05). No signi-ficant differences were found between moxibustion and medication in up-regulating the levels of BMD and bone mineral contents, the load and stiffness(except serum E_2) and down-regulating the levels of the displacement, and concentrations of serum CTX-Ⅰ and TRACP(P>0.05). H.E. staining revealed that rats in the sham operation group showed mild thinness of the bone cortex, uneven thickness of trabecular bone, with distortion, fracture and osteoporosis of the left femur, and different size of rhabdomyocytes in the right quadriceps femoris muscle, with obvious proliferation of interstitial fibrous tissue and inflammatory cell infiltration, which were relatively and clearly milder in both moxibustion and medication groups.Conclusion Moxibustion of BL18 and BL23 can increase the BMD and bone mineral content, improve biomechanical performance, adjust bone metabolism, and mitigate bone and the attached muscle histopathological changes in OP rats, suggesting that modulating interaction between bones and muscles is probably one of the ideas in the treatment of OP.关键词：Osteoporosis;Moxibustion;Biomechanics;Interaction between bones and muscles31|544|9更新时间：2023-08-11
- 摘要：Objective To observe the effect of electroacupuncture(EA) of “Tianshu”(ST25) and “Shangjuxu”(ST37)on gastrointestinal motility, psychological abnormality and expression of RhoA and ROCK protein in rats with diarrhea predominant irritable bowel syndrome(IBS-D), so as to explore its underlying mechanism in improving IBS-D. Methods Thirty-six male rats were randomly and equally divided into control, model, EA and medication groups(n=9 in each group). The IBS-D model with psychological abnormality was established by gavage of dinitrobenzene sulfonic acid(DNBS) + chronic restraint stress. EA(2 Hz/100 Hz, 0.3 mA) was applied to bilateral ST25 and ST37 for 20 min, once a day for 7 days. Rats of the medication group received gavage of pinaverium bromide solution(15 mg/kg), once a day for 7 days. The rats' food intake in 24 h, body mass and colonic contraction waves were recorded. The anhedonia-related behavior was measured using the sucrose consumption test. The elevated plus maze test(the open-arm residence time ratio) was used to assess the anxiety-like behavior. The small intestinal propulsion rate test was used to assess the intestinal motility. The expression levels of RhoA and ROCK proteins in the colonic tissue were measured by Western blot. Results After modeling, the body mass, food consumption, sucrose preference index, the open-arm residence time ratio andlatency of colonic contraction waves were significantly decreased(P<0.05), and the number of contraction waves, intestinal propulsive rate, and the expression levels of RhoA and ROCK proteins considerably increased(P<0.01,P<0.05) in the model group relevant to the control group. Following the interventions, the decrease of body mass, food consumption, sucrose preference index, open-arm residence time ratio and latency of contraction waves, and the increase of the contraction waves, intestinal propulsive rate, and the expression levels of RhoA and ROCK proteins were all reversed by both EA and medication(P<0.05,P<0.01). The effect of EA was significantly superior to that of medication in increasing the sucrose pre-ference index(P<0.05). Conclusion EA can improve both colonic motility and psychological disorders in IBS-D rats with psychological disorder, which may be related to its function in down-regulating the expression of colonic RhoA and ROCK proteins.关键词：Diarrheal predominant irritable bowel syndrome;Electroacupuncture;RhoA;ROCK;Gastrointestinal motility39|631|3更新时间：2023-08-11
- 摘要：Objective To compare the differences in functional connectivity(FC) between the hypothalamus and whole brain regions in patients with premature ovarian insufficiency(POI) and healthy volunteers based on resting-state functional magnetic resonance imaging(rs-fMRI) and investigate the mechanism of acupuncture on treatment of POI. Methods Twelve POI patients were recruited to the acupuncture group and 12 healthy volunteers to the control group. Patients in the acupuncture group received acupuncture at two groups of acupoints alternatively, including Baihui(GV20), Zhongwan(CV12), Shenting(GV24), Shenshu(BL23), Ciliao(BL32) and so on, 30 min once time, 3 times per week for 12 weeks. The state of patients was evaluated by modified Kupperman Index(KI) and self-rating anxiety scale(SAS). Follicle-stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E_2), and anti-mullerian hormone(AMH) were tested by microparticle-based chemiluminescence. B ultrasonography was used to detect the antral follicle count(AFC). Meanwhile, POI patients and healthy volunteers underwent the rs-fMRI examination before and after acupuncture treatment and after enrollment, respectively. With hypothalamus as the region of interest, the differences in FC between the hypothalamus and other brain regions in POI patients and healthy volunteers and the changes of FC between the hypothalamus and whole brain regions in POI patients before and after acupuncture were observed. Results The SAS and KI scores of pre-treatment POI patients were higher than those in the control group(P<0.01).In compa-rison with those pre-treatment, FSH, LH, and SAS and KI scores of POI patients decreased after treatment, while AFC increased(P<0.05). Compared with the control group, the FC of the left hypothalamus with left central sulcus, right middle occipital gyrus, and left paracentral lobule increased, but decreased with left globus pallidus of the lenticular nucleus in POI patients. Furthermore, the FC of the right hypothalamus with the left hippocampus, the left para-central lobule, and the right central sulcus increased, while the FC between the right hypothalamus and the right superior frontal gyrus decreased(P<0.05). For the acupuncture group, compared with the conditions before treatment, the FC of the right hypothalamus with the left inferior frontal gyrus, the left insula, and right inferior frontal gyrus was strengthened, but weakened with the left gyrus rectus(P<0.05). Conclusion The abnormal FC between the hypothalamus and whole brain regions may be one of the central pathological factors of POI. Acupuncture can improve the ovarian function and clinical symptoms of patients with POI, which may be related to its effect in regulating the FC between the hypothalamus and multiple brain regions.关键词：Premature ovarian insufficiency;Acupuncture;Resting-state functional magnetic resonance imaging;Functional connectivity41|518|1更新时间：2023-08-11
- 摘要：Objective To observe the clinical efficacy of balance acupotomy combined with warm needling in treatment of cervical spondylopathy of vertebral artery type(CSA). Methods Ninety patients were randomly divided into a warm needling group, a balance acupotomy group and a combined treatment group, 30 cases in each one. In the warm needling group, warm needling was given, once daily, for 5 days consecutively, with the interval of 2 days every week, and the treatment was conducted for 3 weeks. In the combined treatment group, on the base of the treatment as the warm needling group, the balance acupotomy was exerted, for consecutive 3 weeks. In the balance acupotomy group, the balance acupotomy was adopted, once a week, for 3 weeks consecutively. Clinical efficacy, the mean blood flow velocity of left vertebral artery(LVA), right vertebral artery(RVA) and basilar artery(BA), the pulse index(PI) and the resistance index(RI) were observed in 3 groups separately. The score of vertigo symptom and function was compared before and after treatment in 3 groups. Results In comparison with before treatment, the score of evaluation scale for cervical vertigo(ESCV) and the mean blood flow velocity of LVA, RVA and BA were all increased(P<0.05). while PI and RI reduced(P<0.05) in each group after treatment. In comparison with those in the warm needling group and the balance acupotomy group, ESCV score and the mean blood flow velocity of LVA, RVA and BA were increased(P<0.05), while PI and RI decreased(P<0.05) in the combined treatment group after treatment. Compared with the balance acupotomy group, the mean blood flow velocity of LVA, RVA and BA was increased(P<0.05), and PI and RI reduced(P<0.05) in the warm needling group after treatment. The total effective rate was 73.3%(22/30) in the warm needling group, 70.0%(21/30) in the balance acupotomy group and 93.3%(28/30) in the combined treatment group respectively. The total effective rate in the combined treatment group was higher than that either in the warm needling group or in the balance acupotomy group(P<0.05). Conclusion Balance acupotomy combined with warm needling may restore the mechanical equilibrium state of the neck, and effectively improve the change of vertebral-basilar artery blood flow and relieve vertigo symptoms in patient with of cervical spondylosis of vertebral artery type.关键词：Cervical spondylosis of vertebral artery type;Balance acupotomy;Warm needling;Transcranial Doppler;Hemodynamics55|668|1更新时间：2023-08-11
- 摘要：Objective To observe the acupoint compatibility effect of Shenmen(HT7) and Sanyinjiao(SP6) in improving daytime fatigue and sleepiness of insomnia, and its mechanism in regulating hypothalamic-pituitary-adrenal(HPA) axis and suprachiasmatic nucleus-pineal gland-melatonin(SCN-PG-MT) system. Methods Ninety patients with insomnia were randomly divided into HT7, SP6 and HT7-SP6(HT7 plus SP6) groups, with 30 cases in each group. Electroacupuncture(EA,5 Hz/25 Hz) was applied to HT7, SP6 or HT7-SP6 in each group for 30 min. The EA treatment was conducted once daily, 5 days a week for 2 weeks. Before and after treatment, the Pittsburgh Sleep Quality Index(PSQI) and Epworth Sleepiness Scale(ESS) were observed, separately. The contents of serum adrenocorticotrophic hormone(ACTH), cortisol(CS) and melatonin(MT) were detected by ELISA. Results Compared with before treatment, the sleep quality, sleep time, sleep efficiency, sleep disturbance, daytime dysfunction scores and total score of PSQI in the three groups after treatment were decreased(P<0.05), the time to fall asleep score of PSQI and total score of ESS were decreased in the SP6 and HT7-SP6 groups(P<0.05). After treatment, the sleep quality, time to fall asleep, sleep efficiency, daytime dysfunction scores, total score of PSQI and total score of ESS in the HT7-SP6 group were lower than those in the HT7 group(P<0.05), the sleep quality, sleep efficiency and total score of PSQI in the SP6 group were lower than those in the HT7 group(P<0.05). Compared with before treatment, the serum ACTH and CS levels in the three groups were decreased(P<0.05), and the serum MT levels in the SP6 and HT7-SP6 groups were increased(P<0.05). After treatment, the ACTH and CS levels in the HT7-SP6 group were lower than those in the HT7 group(P<0.05), and the serum MT levels in the SP6 and HT7-SP6 groups were higher than that in the HT7 group(P<0.05). Conclusion The compatibility of HT7 and SP6 has a synergism effect on the improvement of night sleep quality and daytime fatigue and sleepiness of insomnia patients, the mechanism may be related with its function in down-regulating the serum ACTH and CS levels and increasing the serum MT content. SP6 has a better effect than HT7, and plays a major role in acupoint compatibility.关键词：Insomnia;Acupuncture;Adrenocorticotrophic hormone;Cortisol;Melatonin61|954|4更新时间：2023-08-11
- 摘要：Objective To compare the clinical effects on osteonecrosis of the femoral head between the comprehensive therapy(electroacupuncture [EA] of quintuple needling combined with adductor relaxation method) and the adductor relaxation method. Methods Eighty patients with osteonecrosis of the femoral head were randomly allocated into a treatment group and a control group, with 40 patients in each group. The patients in the control group were treated with the adductor relaxation method. In the treatment group, besides the treatment as the control group, EA of quintuple needling was exerted at the most obvious tender sites(Ashi points) around the hip joint, 30 min each time. The treatment was given once daily, 30 days as one session of treatment, and 3 sessions were required totally in each group. The clinical effects of two groups were evaluated. The score of visual analogue scale(VAS), Harris score and the score of the hip mobility were compared before and after treatment and between the two groups. Results The total effective rate was 97.5%(39/40) in the treatment group, higher than 82.5%(33/40) in the control group(P<0.05). Compared with before treatment, the VAS score was remarkably decreased(P<0.05), while the Harris score and the score of the hip mobility were apparently increased in either group after treatment(P<0.05). In comparison between two groups, the VAS score in the treatment group was obviously lower(P<0.05) than that of the control group, while the Harris score and the score of the hip mobility were higher than those of the control group(P<0.05). Conclusion EA of quintuple needling combined with the adductor relaxation method obtains the better clinical effects on ischemic osteonecrosis of the femoral head compared with the simple adductor relaxation method, and this comprehensive therapy obviously relieves the clinical symptoms in patients.关键词：Osteonecrosis of the femoral head;Quintuple needling;Electroacupuncture;Adductor relaxation method32|187|0更新时间：2023-08-11
- 摘要：Objective To observe the effects of electroacupuncture at bilateral Zusanli(ST36), Shangjuxu(ST37), and Sanyinjiao(SP6) acupoints on gastrointestinal function after laparoscopic cholecystectomy under general anesthesia. Methods A total of 150 patients(American Society of Anesthesiologists[ASA] grades Ⅰ and Ⅱ) undergoing laparoscopic cholecystectomy in the Department of Hepatobiliary Surgery of the First Affiliated Hospital of Guangzhou University of Chinese Medicine were randomly assigned into three groups: electroacupuncture group(n=50), simple acupuncture group(n=50), and control group(n=50). Patients in the control group received routine treatment; on the basis of routine treatment, patients in the simple acupuncture group were acupunctured at bilateral ST36, ST37 and SP36;patients in the electroacupuncture group were treated with electroacupuncture at bilateral ST36, ST37 and SP36(50 Hz, 5 mA). In both simple acupuncture group and electroacupuncture group, the corresponding treatments were conducted 1 h after surgery, Patients as well as in the morning(8:00-10:00) and afternoon(14:00-16:00) on the first day after surgery, 30 min each time. The time of bowel sound recovery, the time to the first postope-rative exhaust and defecation, the time of postoperative fluid diet recovery, abdominal pain score, and gastrointestinal reaction score were recorded and analyzed. Results Compared with the control group, the electroacupuncture group and the simple acupuncture group showed shortened time of bowel sound recovery, shortened time to the first postoperative exhaust and defecation, and shortened time of postoperative liquid diet recovery(P<0.01), as well as decreased postoperative abdominal pain score and gastrointestinal reaction score(P<0.01). Furthermore, the time of bowel sound recovery, the time to the first postoperative exhaust and defecation, and the time of postoperative liquid diet recovery were shortened in the electroacupuncture group compared with those in the simple acupuncture group(P<0.01). The total effective rate of electroacupuncture group was 94.0%(47/50), the total effective rate of simple acupuncture group was 88.0%(44/50), significantly higher than 78.0%(39/50) in the control group(P<0.05). Conclusion Both electroacupuncture and simple acupuncture can promote the recovery of gastrointestinal function in patients after laparoscopic cholecystectomy under general anesthesia, and electroacupuncture is superior to simple acupuncture, but they have no significant difference in alleviating postoperative abdominal pain and gastrointestinal reactions.关键词：Laparoscopic cholecystectomy;Electroacupuncture;Acupuncture;Gastrointestinal movement66|402|5更新时间：2023-08-11
- 摘要：Diminished ovarian reserve(DOR) is the main cause of female infertility. Acupuncture has a significant clinical effect on DOR. This article reviews the signal pathways, e.g. PI3 K/Akt, Nrf2/ARE, Bcl-2/Caspase-3 regulated with acupuncture in 3 aspects, i.e. the structure and signal transduction of each pathway, the relationship with ovarian reserve and the improvement of acupuncture in ovarian reserve. It is anticipated to provide a new approach to further exploring the mechanism of acupuncture on the improvement of ovarian reserve. Moreover, the evidences of acupuncture may be obtained for its clinical treatment of DOR and other reproductive endocrine diseases.关键词：Diminished ovarian reserve;Premature ovarian insufficiency;Premature ovarian failure;Acupuncture;Signal pathways83|1137|2更新时间：2023-08-11
- 摘要：A multi-parameter controllable automatic fire-acupuncture instrument was developed by integrating traditional fire needling with modern medical device technology. A gun-like appearance was designed for easy hand-held operation, the electromagnetic induction was for heating needle body, a scale knob was for controlling the needle insertion depth, the combination of electromagnetic ejection and spring return was for the precise control of the needle retention time; and the changeable single ste-rile needle or multiple needles were adopted to meet individual demand, obtain high efficiency and prevent infection. All of these designs are associated with the overall process control system to ensure the exact controllability of needle body temperature, needling density, insertion depth and needle retention time. Besides, this device is advantageous at handy and aseptic operation with high efficiency, conformability and visualization. In this research, this instrument was tested in animals for the impacts of automatic fire needling on skin damage and fur growth. It is found that the accurate control of each parameter is of the significant advantage in the safety and effectiveness of treatment, which lays a solid foundation for the subsequent systematic review on safety and effectiveness.关键词：Fire needle;Automatization;Electromagnetic heating;Electromagnetic ejection18|196|1更新时间：2023-08-11
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