最新刊期
      卷 48 ， 期 10 ， 2023
      • “科技新闻播报：一项关于益元灸对骶髓损伤后逼尿肌无反射型神经源性膀胱大鼠尿流动力学作用的研究取得重要进展。研究通过精心设计的实验，揭示了益元灸在调控膀胱组织中关键分子的表达，如瞬时受体电位香草酸4（TRPV4）、三磷酸腺苷（ATP）、酪氨酸蛋白激酶KIT（C-Kit）和ATP受体P2X5，以促进神经源性膀胱大鼠排尿功能恢复的机制。研究团队首先构建了膀胱逼尿肌无反射型神经源性膀胱模型，并分为不同治疗组进行比较。结果表明，益元灸治疗能够显著改善大鼠的膀胱功能，包括降低最大膀胱容量和膀胱顺应性，提高漏尿点压力等。同时，益元灸还能增加膀胱组织中ATP的含量，提高C-Kit和P2X5的阳性表达率，以及TRPV4、C-Kit、P2X5蛋白的表达水平。这一发现为神经源性膀胱的治疗提供了新的思路和方法。通过激活膀胱组织中的TRPV4通道，益元灸能够促进膀胱上皮细胞释放ATP，从而增加膀胱Cajal间质细胞数量及其嘌呤能受体P2X5表达，提高逼尿肌收缩能力，改善膀胱功能。这一研究不仅为神经源性膀胱的治疗提供了理论依据，也为其他相关疾病的治疗提供了新的启示。该研究不仅推动了神经源性膀胱领域的研究进展，还为该疾病的治疗开辟了新的方向。未来，我们期待看到更多关于益元灸在神经源性膀胱及其他相关疾病治疗中的应用和研究。”
        WEI Hui-lin, ZHANG Zhi-lan, HUANG Xiao-meng, CHEN Jing, REN Ya-feng, LI Bing
        Vol. 48, Issue 10, Pages: 959-968(2023) DOI: 10.13702/j.1000-0607.20221055
        摘要：ObjectiveTo observe the effect of Yiyuan moxibustion on urodynamics and the expressions of transient receptor potential vanilloid 4 （TRPV4）， adenosine triphosphate （ATP）， tyrosine protein kinase KIT （C-Kit） and adenosine triphosphate receptor P2X5 in bladder tissue of rats with detrusor reflex-free neurogenic bladder （NB） after sacral cord injury （SCI）， so as to explore its mechanism in promoting the recovery of urination function of NB rats.MethodsFemale SD rats were randomly divided into sham operation， model， Yiyuan moxibustion， Yiyuan moxibustion+inhibitor （combination） and inhibitor groups， with 12 rats in each group. The model of detruser reflex-free NB after sacral SCI was established by modified Hassan Shaker spinal cord transection method. The behavioral score of Basso Beasttie Bresnahan （BBB） and urodynamic indexes were used to evaluate the model of rats after operation. Fifteen days after modeling， Yiyuan moxibustion was applied to “Shenque” （CV8） and “Guanyuan” （CV4） for 20 min， once daily for 14 days. Rats of the inhibitor and combination groups were given intravesical instillation of HC067047 （1 mL， 1 μmol/L， 30 min）. After the interventions， urodynamics was used to evaluate the bladder function of rats. HE staining was used to observe the morphology of bladder tissue. ATP content in bladder tissue was detected by colorimetric method. The positive expression rates of C-Kit and their receptor P2X5 in bladder tissue were observed by immunofluorescence double labeling method， and TRPV4， C-Kit， and P2X5 protein expression levels in bladder tissue were detected by Western blot.ResultsCompared with the sham operation group， the maximum bladder capacity and bladder compliance of rats in the model group were increased （P<0.01）， the leak point pressure， ATP content， the possitive expression rates of C-Kit and P2X5， and the protein expression levels of TRPV4， C-Kit， P2X5 in bladder tissue were decreased （P<0.01）. In comparison with the model and combination groups， the Yiyuan moxibustion group showed a decrease in maximum bladder capacity and bladder compliance （P<0.01）， an increase in leakage point pressure， ATP content， the possitive expression rates of C-Kit and P2X5， and TRPV4， C-Kit， and P2X5 protein expression levels （P<0.01， P<0.05）； However， these indicators showed opposite trends in the inhibitor group （P<0.01， P<0.05）.ConclusionYiyuan moxibustion can improve the urodynamics and bladder function in rats with bladder detrusor nonreflective after SCI， which may be related to its effect in activating the TRPV4 channel in bladder tissue， promoting the release of ATP from bladder epithelium， thus increasing the expression of bladder Cajal interstitial cells and their purinergic P2X5 receptors.  
        关键词：Sacral cord injury;Neurogenic bladder;Urodynamics;Transient receptor potential vanillin 4;Cajal interstitial cells   
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      • “在神经源性尿潴留的治疗领域取得了重要突破。一项新研究揭示了电针治疗大鼠神经源性尿潴留的分子机制，为这一难题的解决提供了新的视角。研究团队以3-磷酸肌醇依赖性蛋白激酶1（PDK1）/蛋白激酶B（Akt）/超极化激活环核苷酸门控离子通道4（HCN4）通路为切入点，通过精心设计的实验，深入探究了电针治疗对大鼠神经源性尿潴留的影响。实验结果显示，电针刺激“中髎”“中极”能够有效改善大鼠的排尿功能障碍，这一效果与抑制PDK1/Akt通路活化、促进HCN4介导的逼尿肌兴奋性收缩和排尿电信号活化密切相关。该研究不仅为神经源性尿潴留的治疗提供了新的思路，也为针灸等传统医学方法在现代医学领域的应用和发展提供了有力支持。未来，随着对这一机制的深入研究，我们有望为更多患者带来福音。”
        LI Zheng-fei, ZHANG Ren, ZHAO Guo-rui, KUANG Yao
        Vol. 48, Issue 10, Pages: 969-976(2023) DOI: 10.13702/j.1000-0607.20221084
        摘要：ObjectiveTo observe the therapeutic effect of electroacupuncture （EA） on neurogenic urinary retention rats， so as to explore the underlying mechanism of EA in treating neurogenic urinary retention by focusing on 3-phosphoinositide-dependent protein kinase 1 （PDK1）/protein kinase B （Akt）/hyperpolarization activated cyclic nucleotide-gated cation channel 4 （HCN4） pathway.MethodsFemale SD rats were randomly divided into sham operation， model， EA， PDK1 inhibitor， HCN4 blocker and EA + HCN4 blocker groups， with 20 rats in each group. The model of sacral spinal cord injury was established by modified Hassan Shaker spinal cord transection method. EA （2 Hz/15 Hz， 0.5 mA） was applied to “Zhongji” （CV3） and “Zhongliao” （BL33） for 20 min， once daily for 10 days. Rats of the PDK1 inhibitor group received intraperitoneal injection of OSU-03012 （20 mg/kg）， and rats of the HCN4 blocker group received intraperitoneal injection of ivabradine （10 mg/kg）， both once every other day for 10 days. The urodynamic indexes of rats were detected by multi-channel physiological recorder； muscle strip test was used to detect detrusor excitability； the morphological changes of bladder were observed by HE staining. Immunofluorescence double staining was used to detect the co-expression of HCN4 and C-Kit， a specific marker of interstitial cells of Cajal in bladder. Western blot was used to detect the expression of PDK1/Akt/HCN4 pathway proteins in bladder tissue and heat shock protein 27 （HSP27）， a protein related to bladder contraction function.ResultsCompared with the sham operation group， the rats in the model group showed urinary dysfunction， decreased leak point pressure， isolated detrusor spontaneous contraction frequency， fluorescence intensity of C-Kit positive cells， HCN4+/C-Kit+ co-expression， HCN4 and p-HSP27/HSP27 protein expression in bladder tissue （P<0.05）， and increased maximum bladder capacity and comp-liance， minimum tension during contraction of isolated detrusor， PDK1 and p-Akt/Akt protein expression in bladder tissue （P<0.05）. Meanwhile， the above index were all reversed after EA and PDK1 inhibitor intervention （P<0.05）. In comparison with the EA group， the rats had severe urinary dysfunction， the urine leakage point pressure， spontaneous contraction frequency， fluorescence intensity of C-Kit positive cells， the co-expression of HCN4+/C-Kit+， and the protein expression of HCN4 and p-HSP27/HSP27 were decreased （P<0.05）， the maximum bladder capacity and compliance， the minimum tension during contraction of isolated detrusor， and the protein expression of PDK1 and p-Akt/Akt in bladder tissue were increased （P<0.05） in both HCN4 blocker and EA+HCN4 blocker groups. HE staining showed exfoliated bladder epithelium and disordered layers， vacuolization of bladder wall cells， with infiltration of neutrophils in mucosal and muscular layers in the model group， which were relatively milder in the EA and PDK1 inhibitor groups， but worse in the HCN4 blocker and EA + HCN4 blocker groups.ConclusionEA can improve the urinary dysfunction in rats with neurogenic urinary retention， which may be related to its effect in inhibiting the activation of PDK1/Akt pathway， promo-ting HCN4-mediated detrusor excitatory contraction and urinary electrical signal activation.  
        关键词：Electroacupuncture;Neurogenic urinary retention;3-phosphoinositide-dependent protein kinase 1/protein kinase B pathway;Hyperpolarization activated cyclic nucleotide-gated cation channel 4   
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      • “电针对骶上脊髓损伤后逼尿肌反射亢进型大鼠膀胱功能的改善作用及其机制研究取得了新进展。本研究通过对比空白组、假手术组、模型组、电针组和电针+PD98059组大鼠的尿流动力学参数、膀胱组织形态、脊髓组织细胞凋亡情况以及相关信号通路的表达水平，发现电针治疗能够显著降低膀胱基础压、漏尿点压和最大压力，增加膀胱最大容量和顺应性，减少脊髓组织细胞凋亡，并上调脊髓组织中Epac2、Rap、p-Raf、p-MEK、p-ERK1/2及Bcl-2蛋白的表达水平，下调Bax蛋白的表达水平。这些结果表明，电针可能通过上调脊髓Epac2、Rap，启动Raf-MEK-ERK级联反应，减少细胞凋亡，从而改善逼尿肌反射亢进型大鼠的膀胱功能。这一发现为骶上脊髓损伤后膀胱功能障碍的治疗提供了新的思路和方法。”
        XU Ming, AI Kun, DENG Shi-feng, LIU Qiong, ZHAN Li-fen, CHEN Xiao-wen, LI Ya, KUANG Jing-zhi, ZHANG Hong
        Vol. 48, Issue 10, Pages: 977-985(2023) DOI: 10.13702/j.1000-0607.20230233
        摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on urodynamics and Raf/MEK/ERK signaling pathway in spine cord tissue of rats after suprasacral spinal cord injury （SSCI）， so as to explore its possible mechanism in improving bladder function in rats with detrusor hyperreflexia after SSCI.MethodsFemale SD rats were randomly divided into blank， sham operation， model， EA and EA+PD98059 groups， with 12 rats in each group. Thorax （T） 10 spinal cord transection was performed by surgery. Rats in the EA group were given EA （10 Hz/50 Hz， 20 min） at “Ciliao” （BL32）， “Zhongji” （CV3）， “Sanyinjiao” （SP6） and “Dazhui” （GV14） once daily for 7 d. Rats of the EA+PD98059 group received intraperitoneal injection of PD98059 （5 mg/kg） 2 h before EA intervention. The urodyna-mics was used to measure the base pressure， leak point pressure， maximum pressure， maximum capacity and comp-liance of bladder， and the morphology of bladder detrusor tissue was observed with HE staining. The TUNEL staining was used to detect the cell apoptosis of the spinal cord tissue. The expression levels of exchange protein directly activated by cAMP 2 （Epac2）， Rap， phosphorylated rapidly accelerated fibrosarcoma （p-Raf）， phosphorylated mitogen-activated extracellular signal-regulated kinase （p-MEK）， phosphorylated extracellular signal regulated kinase 1 and 2 （p-ERK1/2）， B-cell lymphoma-2 （Bcl-2）， and Bcl-2 associated X protein （Bax） were determined by Western blot.ResultsCompared with the sham operation group， the base pressure， leak point pressure and maximum pressure of bladder were significantly increased （P<0.01）， the maximum bladder capacity and bladder compliance were decreased （P<0.01）， the cell apoptosis rate of spinal cord tissue was increased （P<0.01）， and the expression levels of Epac2， Rap， p-Raf， p-MEK， p-ERK1/2， and Bcl-2 protein in spinal cord tissue were decreased （P<0.01）， while the expression level of Bax protein was increased （P<0.01） in the model group. After the treatment and compared with the model group， the base pressure， leak point pressure and maximum pressure of bladder， the cell apoptosis rate of spinal cord tissue， the expression level of Bax protein were decreased （P<0.05） in the EA group， while the maximum bladder capacity and bladder compliance， the expression levels of Epac2， Rap， p-Raf， p-MEK， p-ERK1/2， and Bcl-2 protein in spinal cord tissue were all increased （P<0.05， P<0.01）. In comparison with the EA group， the base pressure， leak point pressure and maximum pressure of bladder， the cell apoptosis rate， the expression level of Bax protein were significantly increased （P<0.05）， whereas the maximum bladder capacity， bladder compliance， and the expression levels of p-MEK， p-ERK1/2， and Bcl-2 protein were decreased （P<0.05） in the EA+PD98059 group. Results of HE staining showed disordered transitional epithelial cells and destroyed lamina propria in bladder detrusor tissue， with the infiltration of monocytes in the model group， which was obviously milder in both EA and EA+PD98059 groups， especially in the EA group.ConclusionEA can improve the bladder function in detrusor hyperreflexia rats after SSCI， which may be related to its effect in up-regulating Epac2 and Rap， activating the Raf-MEK-ERK pathway， and reducing the cell apoptosis of spinal cord tissue.  
        关键词：Electroacupuncture;Suprasacral spinal cord injury;Neurogenic bladder;Raf/MEK/ERK signaling pathway;Urodynamics   
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      • “一项研究观察了运动头针法对卒中后痉挛（PSS）大鼠H-反射变化的影响，并探讨了其抗PSS的电生理机制。研究发现，运动头针法能够有效改善神经功能损伤，降低痉挛肌运动阈值、Hmax/Mmax比值及H-反射FDD，发挥较好的抗PSS效应。这一发现为PSS的治疗提供了新的思路和方法，具有重要的临床价值。该研究采用了雄性SD大鼠作为实验对象，通过改良线栓法制备大脑中动脉闭塞模型，并随机分为假手术组、模型组和运动头针组。经过连续7天的治疗后，研究人员发现模型组大鼠的神经功能损伤、肌张力及运动功能均出现了显著变化，而运动头针组则呈现出相反的结果。这一结果表明，运动头针法具有显著的抗PSS作用。此外，研究人员还记录了各组大鼠的H-反射变化，发现模型组大鼠的H-反射频率依赖性抑制（FDD）显著升高，而运动头针组则呈现出相反的趋势。这一发现进一步证实了运动头针法能够改善神经功能损伤，降低痉挛肌运动阈值、Hmax/Mmax比值及H-反射FDD，从而发挥抗PSS作用。该研究不仅为PSS的治疗提供了新的思路和方法，同时也为运动头针法的应用和发展提供了有力支持。未来，我们可以进一步探索运动头针法的临床应用，为更多的患者带来福音。”
        ZHANG Qin-yong, MA Liang-xiao, WANG Jun-xiang, MA Xue-ling, MU Jie-dan, QIAN Xu, MA Ling-hui, ZHANG Zhou, SUN Tian-yi, ZHANG Yi-dan, LIU Zi-zhen
        Vol. 48, Issue 10, Pages: 986-992(2023) DOI: 10.13702/j.1000-0607.20220920
        摘要：ObjectiveTo observe the effect of motion-style scalp acupuncture （MSSA） on H-reflex in rats with post-stroke spasticity （PSS）， so as to explore the electrophysiological mechanisms of MSSA against spasticity.MethodsA total of 36 male SD rats were randomly divided into sham operation， model and MSSA groups， with 12 rats in each group. The stroke model was established by occlusion of the middle cerebral artery. After modeling， rats in the MSSA group were treated by scalp acupuncture （manipulated every 15 min， 200 r/min） at ipsilesional “parietal and temporal anterior oblique line” （MS6） for a total of 30 min， the treadmill training （10 m/min） was applied during the needling retention， once daily for consecutive 7 days. The neurological deficits， muscle tone and motor function were assessed by Zea Longa score， modified modified Ashworth scale （MMAS） score and screen test score before and after treatment， respectively. The H-reflex of spastic muscle was recorded by electrophysiological recordings and the frequency dependent depression （FDD） of H-reflex was also recorded. The cerebral infarction volume was evaluated by TTC staining.ResultsCompared with the sham operation group， the Zea longa score， MMAS score， cerebral infarction volume， motion threshold， Hmax/Mmax ratio and FDD of H-reflex were significantly increased （P<0.01）， while the screen test score was significantly decreased （P<0.01） in the model group. Intriguingly， compared with the model group， the above results were all reversed （P<0.01） in the MSSA group.ConclusionMSSA could exert satisfactory anti-spastic effects in rats with PSS， the underlying mechanism may be related to the improvement of nerve function injury， the reduction of spastic muscle movement threshold， Hmax/Mmax ratio and H-reflex FDD.  
        关键词：Post-stroke spasticity;Motion-style scalp acupuncture;Acupuncture technique;Electrophysiology;H-reflex   
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      • “在关节炎治疗领域取得重要进展。研究团队通过艾灸“足三里”对膝关节骨关节炎（KOA）和类风湿性关节炎（RA）大鼠进行治疗，并深入观察了膝关节滑膜巨噬细胞极化情况。实验结果显示，艾灸对AKO和RA大鼠均能有效减轻膝关节炎性损伤，调控炎性反应。对于；KO而对于ARA大鼠大鼠，，艾灸艾灸主要通过则促进抑制MM21型型巨噬细胞巨噬细胞极化极化，，提高降低抑促有力炎炎因子因子ILIL--110β的含量的水平。这一发现可能揭示了“足三里”穴位对不同机体状态下的靶向性调节机制。这项研究不仅为关节炎的治疗提供了新的思路，也为中医针灸理论的发展提供了支持。未来，艾灸在关节炎治疗领域的应用潜力值得期待。”
        WANG Cheng, YUAN Jun, GUO Yan-ding, LUO Kun, YU Shu-guang, LUO Ling, ZHOU Hai-yan
        Vol. 48, Issue 10, Pages: 993-1000(2023) DOI: 10.13702/j.1000-0607.20220952
        摘要：ObjectiveTo observe the similarities and differences of effects of moxibustion at “Zusanli” （ST36） on target tissues and macrophages polarization in knee osteoarthritis （KOA） and rheumatoid arthritis （RA） rats， and to summarize its efficacy and characteristics.MethodsThirty rats were equally and randomly divided into control， KOA， RA， KOA+Moxi and RA+Moxi groups. The KOA model and RA model were induced by injection of sodium monoiodoacetate or Freund’s complete adjuvant into the rats’ knee joints， respectively. Rats of the KOA+Moxi and RA+Moxi groups received moxibustion stimulation at bilateral ST36 for 30 min， once a day for 21 days， beginning from the 7th day on after modeling. The contents of serum interleukin（IL）-1β and IL-10 were detected by ELISA. Histopathological changes （Markin score of the knee cartilage and synovial pathology score） of the knee joints were observed after HE staining. The polarization state of M1 and M2 macrophages in the synovial tissue of the knee joints was assessed by detecting the expression of CD86 and CD206 after immunofluorescence staining.ResultsCompared with the control group， the content of serum IL-1β， synovial pathology score， and synovial CD86 expression were significantly increased （P<0.01， P<0.05）， while the content of serum IL-10 and synovial CD206 expression markedly decreased （P<0.01） in both KOA and RA groups； the Markin score was increased （P<0.01） in the KOA group. In comparison with the KOA group， the Markin score was obviously decreased （P<0.01）， while the content of serum IL-10 and CD206 expression were apparently increased （P<0.01） in the KOA+Moxi group. No significant changes were found in the content of serum IL-1β， synovial pathology score and CD86 expression in the KOA+Moxi group relevant to the KOA group. In comparison with the RA group， the content of serum IL-1β， synovial pathology score， and CD86 expression were considerably decreased （P<0.01） in the RA+Moxi group. No marked differences were found in the serum IL-10 level， Markin score， and CD206 expression between RA+Moxi and RA model groups. The increased Markin score was significantly higher in the KOA group than in the RA group （P<0.01）， but the increased synovial pathology score was significantly lower in the KOA group than in the RA group （P<0.01）. Correspondingly， the effect of moxibustion at ST36 was significantly better in RA model than in KOA model in reducing serum IL-1β （P<0.05）.ConclusionMoxibustion at ST36 can effectively reduce cartilage injury of knee joint in rats with KOA and reduce synovial injury in rats with RA， which may be related with its effects in lowering IL-1β level in RA model by inhibiting the polarization of M1 macrophages， and up-regulating level of IL-10 in KOA model by promoting the polarization of M2 macrophages. However， the relevant mechanism needs to be further studied.  
        关键词：Moxibustion;Zusanli （ST36）;Knee osteoarthritis;Reheumatoid arthritis;Macrophage polarization;Efficacy of acupoints   
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      • “一项关于电针“夹脊”治疗腰椎间盘退变的研究取得了重要进展。该研究通过对比不同组别的兔子在接受电针治疗后的情况，发现电针“夹脊”有助于改善腰椎间盘退变模型兔的生长和运动功能。研究结果表明，与模型组相比，电针组大白兔在治疗后每周体质量增长加快，运动功能评分升高，这显示出电针“夹脊”对兔子生长和运动功能的积极影响。同时，研究还发现电针“夹脊”能够减少髓核细胞及纤维环组织Cav-1阳性细胞数，增强髓核细胞端粒酶活性，延长髓核细胞相对端粒长度，并改善髓核细胞周期停滞，从而延缓腰椎间盘退变的髓核细胞衰老。这项研究为电针“夹脊”治疗腰椎间盘退变提供了科学依据，为相关疾病的治疗提供了新的思路和方法。未来，随着研究的深入，电针“夹脊”治疗腰椎间盘退变的应用前景将更加广阔。”
        ZHANG Yan-lin, ZOU Jing, WANG Min, WANG Kun-xiu, HUANG Guo-fu
        Vol. 48, Issue 10, Pages: 1001-1008(2023) DOI: 10.13702/j.1000-0607.20220614
        摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Jiaji”（EX-B2） on body mass， motor function， expression of caveolin-1 （Cav-1） in nucleus pulposus cells and annulus fibrosus tissue， telomerase activi-ty， relative telomere length and different cell cycle ratio of nucleus pulposus cells in rabbits with intervertebral disc degeneration（IVDD）， so as to investigate its mechanism underlying delaying senescence of the degenerated lumbar intervertebral disc nucleus pulposus cells.MethodsTwenty-five male New Zealand rabbits with mature bones were divided into control， sham operation， model， EA， and acupuncture groups， with 5 rabbits in each group. The IVDD model was established by inserting kirschner wires to the vertebral bone surface between the lumbar （L）4 and L5 vertebrae， followed by applying continuous axial pressure for 28 d. EA （2 Hz/15 Hz， 1—2 mA） or acupuncture （only insertion of acupuncture needles into bilateral EX-B2， but without electrical stimulation） was applied to bilateral EX-B2 for 20 min， once daily， 6 times a week for 4 weeks. The hindlimb locomotor function （locomotor score） was assessed by using Faden’s and colleagues’ methods. The general conditions of rabbits in each group were observed， and their body weight changes were measured every week. Nucleus pulposus cells were isolated using enzyme digestion method. After the treatment， the Cav-1 positive cell counts in nucleus pulposus cells and annulus fibrosus tissues were detected by immunohistochemistry， and the telomerase activity of nucleus pulposus cells was detected by PCR-ELISA. The relative telomere length of nucleus pulposus cells was measured by real-time quantitative polymerase chain reaction （real-time qPCR）， and the cell cycle of nucleus pulposus was detected by flow cytometry.ResultsCompared with the sham operation group， the body mass from 4 to 11 week， locomotor score at 4， 7 and 11 week， telomerase activity， relative telomere length and the proportion of cells in G2/M phase of nucleus pulposus cells were significantly decreased （P<0.01）， while Cav-1 positive cell counts of nucleus pulposus and annulus fibrosus tissue， and the proportion of nucleus pulposus cells in the G0/G1 phase considerably increased （P<0.01） in the model group. Relevant to the model group， the EA group rather than the acupuncture group had an increase in the body mass from 8 to 11 week， locomotor score at 11 week， telomerase activity， relative telomere length of nucleus pulposus cells， and the proportion of nucleus pulposus cells in G2/M phase （P<0.01）， and a decrease in the Cav-1 positive cell counts of nucleus pulposus and annulus fibrosus tissue and the proportion of cells in G0/G1 phase （P<0.01）. No significant differences were found between the model and acupuncture groups in all the indexes mentioned above.ConclusionEA at EX-B2 has a bene-ficial effect in improving motor function in rabbits with IVDD， which may be related to its functions in reducing the expression of Cav-1 in nucleus pulposus cells and annulus fibrosus， improving cycle arrest， enhancing the telomerase activity and the relative telomere length of nucleus pulposus cells， delaying the senescence of nucleus pulposus cells of the degenerated lumbar intervertebral discs.  
        关键词：Intervertebral disc degeneration;Electroacupuncture;Cell senescence;Caveolin-1;Telomeres;Cell cycle   
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      • “一项关于电针对肌萎缩侧索硬化症（ALS）小鼠影响的研究取得了重要进展。研究人员通过对比模型组、电针组和利鲁唑组小鼠的运动功能、脊髓细胞外亲环素A（PPIA）及相关信号通路蛋白的表达，深入探讨了电针干预调节细胞外PPIA对ALS小鼠神经炎性的作用机制。实验结果显示，电针干预能够有效改善ALS小鼠的运动功能，减轻脊髓前角运动神经元的损伤，降低脊髓组织TDP-43蛋白的表达及阳性表达，同时降低脊髓组织PPIA和NF-κB蛋白的相对表达量以及血清PPIA含量和脊髓组织PPIA免疫荧光表达。这些发现为电针在ALS治疗中的应用提供了重要依据，揭示了电针干预调节细胞外PPIA对ALS小鼠神经炎性的重要作用机制。这项研究不仅为ALS的治疗提供了新的思路和方法，也为针灸等传统医学手段在神经系统疾病治疗中的应用提供了有力支持。未来，研究人员将进一步深入探索电针干预治疗ALS的具体机制，以期为患者带来更有效的治疗方法。”
        LIU Jun-yang, LU Yuan-rong, GUO Jie, LI Hua, WANG Yuan, ZHAO Ying-qian, LI Jie, WANG Qiang
        Vol. 48, Issue 10, Pages: 1009-1016(2023) DOI: 10.13702/j.1000-0607.20230251
        摘要：ObjectiveTo observe the effects of electroacupuncture （EA） on motor function， expression of extracellular cyclophile A（PPIA） and PPIA/nuclear factor-κB （NF-κB） signaling pathway in spinal cord of amyotrophic la-teral sclerosis （ALS） mice，so as to explore the mechanism of EA intervention in regulating extracellular PPIA on neuroinflammation in ALS mice.MethodsThirty ALS-SOD1G93A mice with hSOD1-G93A gene were randomly divided into model， EA and Riluzole groups ，with 10 mice in each group， and other 10 ALS-SOD1G93A negative mice were used as the blank group. EA was applied to bilateral “Yanglingquan”（GB34） and “Zusanli”（ST36） for 20 min once daily， 5 days a week for 2 weeks. In the Riluzole group， riluzole solution （30 mg·kg-1·d-1） was administrated intragastrically， and the treatment time was the same as that in the EA group.Rotating rod experiment and open field experiment were used to evaluate the changes in motor function of mice .The morphology of motor neurons in the anterior horn of spinal cord was observed by HE staining.The relative protein expression levels of PPIA， TDP-43 and NF-κB in the spinal cord were detected by Western blot.The positive expression level of TDP-43 in the spinal cord was detected by immunohistochemistry. The positive expression level of PPIA in spinal cord was marked by immunofluorescence. Serum PPIA content was determined by ELISA.ResultsCompared with the blank group， the time of rod dropping and the total distance of open field movement in the model group were shortened （P<0.01）， the number of motor neurons in the anterior horn of the spinal cord was reduced， the cell morphology was incomplete， the cell body was atrophied， the protein expression and positive expression of TDP-43 were increased （P<0.01）， the protein expressions of PPIA and NF-κB in the spinal cord were increased（P<0.01），the serum content of PPIA and immunofluorescence expression of PPIA in spinal cord were increased （P<0.01）. Compared with the model group， the time of rod dropping and the total distance of open field movement of mice in the EA group and the Riluzole group were prolonged （P<0.05，P<0.01）， and the injury of motor neuron in the anterior horn of the spinal cord was decreased， the protein expression and positive expression of TDP-43 in the spinal cord were decreased （P<0.05，P<0.01）； the relative expression levels of PPIA and NF-κB proteins were decreased （P<0.05， P<0.01）， and the content of PPIA in serum and the immunofluorescence expression of PPIA in the spinal cord were decreased （P<0.05， P<0.01） in the EA group； the relative protein expression of NF-κB and fluorescence expression of PPIA in spinal cord of mice in the Riluzole group were decreased （P<0.05）.ConclusionEA intervention can improve motor function in ALS mice， and its mechanism may be related to the inhibition of PPIA/NF-κB signaling pathway by EA to alleviating neuroinflammatory response.  
        关键词：Amyotrophic lateral sclerosis;Electroacupuncture;Extracellular cyclophilin A;Nuclear factor-κB   
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      • “神经生长因子（NGF）/磷脂酰肌醇3-激酶（PI3K）/瞬时受体电位香草酸受体1（TRPV1）信号通路在针刺“上巨虚”治疗肠易激综合征（IBS）慢性内脏痛敏的作用机制方面取得了重要突破。研究人员通过建立成年慢性内脏痛敏大鼠模型，并运用腹壁回撤反射（AWR）评分、Western blot和实时荧光定量PCR等多种技术手段，深入探讨了针刺“上巨虚”对IBS慢性内脏痛敏的镇痛作用机制。实验结果显示，与正常组相比，模型组大鼠在不同压力等级下的AWR评分以及结肠组织中的NGF、TrkA、PI3K、TRPV1的mRNA和蛋白表达均显著升高。而在针刺“上巨虚”干预后，这些指标均显著降低，表明针刺“上巨虚”对IBS慢性内脏痛敏具有镇痛作用。这一发现为针刺治疗IBS提供了新的理论依据，也为针灸在慢性内脏痛敏治疗中的应用提供了更多可能性。该研究成果不仅为神经生长因子/磷脂酰肌醇3-激酶/瞬时受体电位香草酸受体1信号通路在针刺治疗IBS中的作用机制提供了重要依据，也为针灸在临床实践中的应用推广提供了有力支持。未来，随着对这一领域的深入研究，相信针灸治疗IBS的效果将得到进一步提升，为广大患者带来福音。”
        CHEN Cai-yi, YU Zhi, JI Feng, HE Ling-ling, WANG Xuan, XU Bin
        Vol. 48, Issue 10, Pages: 1017-1024(2023) DOI: 10.13702/j.1000-0607.20221021
        摘要：ObjectiveTo investigate the effect of manual acupuncture at “Shangjuxu”（ST37） on nerve growth factor（NGF）/phosphatidylinositol 3-kinase（PI3K）/transient receptor potential vanilloid 1（TRPV1） signaling pathway in rats with chronic visceral hyperalgesia of irritable bowel syndrome （IBS）， so as to explore its underlying mechanism in treating IBS chronic visceral hyperalgesia.MethodsIBS chronic visceral hyperalgesia model was established by colorectal dilation stimulation for 2 weeks for SD pups at 8 d after birth， which were fed until 8-week age after the stimulation. Then the verified successfully modeled adult rats were randomly divided into model， Shangjuxu， and non-acupoint groups， with 6 rats in each group， and 6 unmodeled rats were selected as normal group. On the next day of model evaluation， rats in the Shangjuxu group received acupuncture at right ST37 while rats in the non-acupoint group received acupuncture at the non-meridian and non-acupoint point in the right hypochondrium， both for 15 min， with manual twisting of mild reinforcing and reducing performed for 30 s at an interval of 5 min， once a day， for a total of 7 d. Abdominal withdrawal reflex（AWR） score was used to evaluate the degree of chronic visceral pain in rats. Western blot and real-time fluorescence quantitative PCR were used to detect the colonic protein and mRNA expressions of NGF， tropomyosin receptor kinase A （TrkA）， PI3K and TRPV1. The positive expressions of PI3K and TRPV1 proteins in the colon of rats were detected by immunohistochemistry method.ResultsCompared with the normal group， AWR scores corresponding to 4 pressure levels of 20， 40， 60 and 80 mm Hg， mRNA and protein expressions of NGF， TrkA， PI3K and TRPV1 in colon tissue， and positive expressions of PI3K and TRPV1 in colon tissue were significantly increased（P<0.05） in the model group. After intervention， compared with the model group， rats in the Shangjuxu group had reduced AWR scores corresponding to 4 pressure levels of 20， 40， 60 and 80 mm Hg， lower colonic mRNA and protein expressions of NGF， TrkA， PI3K and TRPV1， and decreased positive expressions of PI3K and TRPV1 in colon tissue（P<0.05）， while there were no significant differences in the above indexes of the non-acupoint group.ConclusionManual acupuncture at ST37 can alleviate IBS chronic visceral hyperalgesia in rat and its analgesic effect may be related to regulating NGF/PI3K/TRPV1 signaling pathway.  
        关键词：Acupuncture;Chronic visceral hyperalgesia;Irritable bowel syndrome;Nerve growth factor/phosphatidylinositol 3-kinase/transient receptor potential vanilloid 1 signaling pathway;Shangjuxu（ST37）   
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      • “一项关于针刺治疗水液缺乏型干眼（ATD）的研究取得了重要进展。该研究观察了针刺对豚鼠眼表症状及泪腺组织中血管活性肠肽（VIP）/环磷酸腺苷（cAMP）/蛋白激酶A（PKA）/水通道蛋白5（AQP5）信号通路相关蛋白表达的影响，探讨了针刺治疗ATD的作用机制。研究结果显示，针刺能够减轻干眼眼表损伤，促进泪液分泌。这一作用机制可能与激活VIP/cAMP/PKA/AQP5信号通路，增强泪腺分泌功能有关。此外，研究还发现针刺组和西药组在治疗ATD方面均有一定的效果，但针刺组在促进泪液分泌方面表现出更好的效果。这一研究不仅为针刺治疗ATD提供了理论基础，也为干眼症的治疗提供了新的思路和方法。未来，我们期待更多的研究能够进一步验证和完善这一治疗体系，为患者带来更好的治疗效果。”
        LIU Xue, LI Ying, SHEN Hu-xing, GAO Wei-ping, ZHAO Nan, LI Liu-jiao, LIU Cheng-yong
        Vol. 48, Issue 10, Pages: 1025-1032(2023) DOI: 10.13702/j.1000-0607.20230061
        摘要：ObjectiveTo observe the effect of acupuncture on the ocular surface symptoms and the protein expression of vasoactive intestinal peptide （VIP） / cyclic adenosine monophosphate （cAMP）/protein kinase A （PKA） / aquaporin 5（AQP5） signaling pathway in lacrimal gland tissue of aqueous tear deficiency （ATD） type dry eye model，so as to investigate its mechanism underlying improvement of ATD.MethodsBritish shorthair guinea pigs were randomly divided into blank control， model， acupuncture， sham-acupuncture and medication group，with 8 guinea pigs in each group. The ATD model was established by subcutaneous injection of scopolamine hydrobromide （0.6 mg/dose，4 times/d for 10 days）. For guinea pigs of the acupuncture group， filiform needles were inserted into bilateral “Jingming”（BL1）， “Cuanzhu”（BL2）， “Sizhukong”（TE23）， “Taiyang”（EX-HN5），and “Tongziliao”（GB1） for 15 min. For guinea pigs of the sham-acupuncture group， a blunt filiform needle was used to repeatedly prick （not pierce） the skin of the same acupoints mentioned above. The treatment in the above two groups was conducted once daily for 14 days. The guinea pigs in the medication group received administration of sodium hyaluronate eye drops in both eyes， three times a day for 14 days. The objective tests of tear film break-up time （BUT）， corneal fluorescein staining score （FLS） and phenol red thread （PRT） test were conducted before and after modeling and after the intervention. After the intervention， the lacrimal index （weight of lacrimal gland/body weight） was calculated. Histopathological changes of the lacrimal gland were observed after H.E. staining. The expression of AQP5 in the lacrimal gland were detected by immunofluorescence， and the contents of VIP and AQP5 in the lacrimal gland were measured by ELISA， the protein expression levels of VIP， cAMP， PKA， p-PKA and AQP5 in the lacrimal gland were detected by Western blot.ResultsIn comparison with the blank control group， the PRT， BUT， lacrimal index， AQP5 immunoactivity， contents of VIP and AQP5， and protein expression levels of VIP， cAMP， PKA， p-PKA and AQP5 were significantly decreased（P<0.01，P<0.05）， and FLS was obviously increased （P<0.01） in the model group . Compared to the model group， the PRT， BUT， lacrimal index， AQP5 immunoactivity， contents of VIP and AQP5， and expression levels of VIP and AQP5 in both acupuncture and medication groups， and the expression levels of cAMP， PKA， p-PKA in the acupuncture group were considerably increased （P<0.01， P<0.05）， while the FLS was markedly decreased in both acupuncture and medication groups （P<0.01， P<0.05）. Compared with the medication group， the acupuncture group had increased PRT （P<0.05）.ConclusionAcupuncture intervention is effective in reducing ocular surface damage and promoting tear secretion in guinea pigs with ATD， which may be related to its function in activating VIP/cAMP/PKA signaling， and promoting the expression of AQP5 in the lacrimal gland.  
        关键词：Acupuncture;Aqueous tear deficiency dry eye;Lacrimal gland;VIP/cAMP/PKA/AQP5 signaling pathway   
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      • “一项关于电针“肠病方”在改善大鼠溃疡性结肠炎（UC）机制的研究取得了重要进展。研究团队通过建立UC模型，观察电针对大鼠结肠巨噬细胞极化的影响，揭示了电针在调节结肠巨噬细胞极化、改善UC症状方面的潜力。研究结果显示，电针组大鼠在接受电针治疗后，其结肠病理组织的损伤程度和炎性细胞浸润均得到了显著改善。同时，电针还显著提高了大鼠体质量增长幅度、结肠长度、脾质量以及血清中抑炎因子IL-2、IL-10的含量。这些指标的改善表明电针在缓解UC症状、减轻炎性反应方面发挥了积极作用。值得一提的是，电针组大鼠结肠中M2型巨噬细胞标记物Arg1的表达水平也显著升高，而M1型巨噬细胞标记物iNOS的表达水平则显著降低。这一发现揭示了电针调节结肠巨噬细胞极化的机制，即通过抑制M1型巨噬细胞的生成、促进M2型巨噬细胞的生成来改善UC症状。该研究的成果不仅为溃疡性结肠炎的治疗提供了新的思路和方法，也为中医针灸技术在现代医学中的应用提供了新的依据和支持。”
        XU Hui-chao, WU Rong-lin, JIANG Zi-wen, WANG Hai-jun, CAO Yu-xia, HAO Jian-heng, LI Rang-qian, JI Lai-xi
        Vol. 48, Issue 10, Pages: 1033-1040(2023) DOI: 10.13702/j.1000-0607.20230286
        摘要：ObjectiveTo investigate the effect of electroacupuncture（EA） at “Changbing Decoction” on alleviating ulcerative colitis （UC） and regulating the polarization of colonic macrophages in rats， so as to explore its mechanisms underlying improvement of UC.MethodsTwenty-six male SD rats were randomly divided into 4 groups： normal group（6 rats）， model group（8 rats）， EA group（6 rats）， and western medication group（6 rats）. The rat model of UC was established by using 5% dextran sulfate sodium （DSS） solution drinking water for 7 days， followed by drinking 1% DSS solution during treatment period. After 7-day model establishment， EA treatment（10 Hz/50 Hz， 20 min） was applied to “Zhongwan”（CV12）， bilateral “Tianshu”（ST25） and “Shangjuxu”（ST37） for 3 d， and rats in the western medication group were given mesalazine suspension（200 mg/kg） by gavage for 3 d. The body weight， spleen weight and colon length of rats were measured. The disease activity index （DAI） score was evaluated. The morphological changes and inflammatory cell infiltration of colon were detected after HE staining and pathological scores were eva-luated. The contents of tumor necrosis factor α（TNF-α）， interleukin（IL）-1β， IL-2 and IL-10 in serum were detected by ELISA. The protein expressions of M1 and M2 macrophage markers nitric oxide synthase （iNOS） and arginase 1（Arg1） were detected by fluorescence double staining and Western blot， respectively. Quantitative real-time PCR was used to detect iNOS and Arg1 mRNA expressions.ResultsCompared with the normal group， rats in the model group had increased pathological damage degree and inflammatory cell infiltration in the colon tissue， slowed-down body weight gain， decreased colon length， spleen weight， serum anti-inflammatory factors IL-2 and IL-10 contents， colonic Arg1/CD68 fluorescence positive expression， and Arg1 protein and mRNA expressions（P<0.01， P<0.05）， as well as increased DAI scores， colon histopathological scores， contents of serum pro-inflammatory factors TNF-α and IL-1β， colonic iNOS/CD68 fluorescence positive expression， iNOS protein and mRNA expressions（P<0.01）. Compared with the model group， the above indicators were significantly improved in rats of the EA group and the western medication group（P<0.01， P<0.05）.ConclusionEA of “Changbing Decoction” can improve UC of rats by regulating the polarization of colonic macrophages， inhibiting the generation of M1 macrophages and promoting the generation of M2 macrophages.  
        关键词：Ulcerative colitis;Electroacupuncture;Macrophages;Polarization   
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      • “在帕金森病（PD）治疗领域，最新的研究揭示了电针对PD小鼠肠道屏障功能及炎性反应的积极影响及其作用机制。本研究通过对比对照组、模型组和电针组小鼠的行为学评分、自主运动总路程以及结肠组织中的关键分子表达水平，发现电针“风府”“太冲”“足三里”能有效清除PD小鼠中脑黑质区和结肠组织中异常聚集的α-突触核蛋白（α-syn），改善肠道屏障受损，并调节硫氧还蛋白互作蛋白（TXNIP）/NOD样受体3（NLRP3）信号通路。这些发现不仅为PD的治疗提供了新的视角，也为针灸在神经系统疾病中的应用提供了科学依据。”
        WANG Yao, WANG Yan-chun, MA Jun, LI Ya-nan, ZHANG Xiao-lei, HU Meng-ni, QI Ling, GUO Lei, RONG Zhen, GUAN Qing-ya
        Vol. 48, Issue 10, Pages: 1041-1047(2023) DOI: 10.13702/j.1000-0607.20220554
        摘要：ObjectiveTo observe the effects of electroacupuncture （EA） at “Fengfu” （GV16）， “Taichong” （LR3） and “Zusanli” （ST36） on α-synuclein （α-syn）， Occludin， Claudin-1， thioredoxin interaction protein （TXNIP） and Nod-like receptor 3 （NLRP3） in Parkinson’s disease （PD） mice， so as to investigate the mechanisms of EA on intestinal barrier function and inflammation in PD mice.MethodsThirty six C57BL/6 mice were randomly divided into control， model and EA groups， with 12 mice in each group. PD mice model was induced by rotenone intragastric administration for 28 days. Mice in the EA group were treated with EA （2 Hz， 1 mA） at GV16， LR3 and ST36 for 30 min， once a day for 14 days. The behavioral scores were observed. The total distance of autonomic movement was measured by open field test. The expression level of α-syn in substantia nigra and colon tissue was determined by immunohistochemistry. The colonic morphology and goblet cell distribution were observed by Alcian blue staining. The expression levels of Occludin， Claudin-1， TXNIP and NLRP3 mRNA in colon tissue were detected by real-time fluorescence quantitative PCR.ResultsCompared with the control group， the behavioral scores of rats were increased （P<0.01）； the total distance of autonomous movement was decreased （P<0.01）； the positive expression level of α-syn in the substantia nigra and colon was increased （P<0.01）； the goblet cells and crypts in colon tissue were reduced， and the muscular layer was thinner； the expression levels of Occludin and Claudin-1 mRNAs in colon tissue were decreased （P<0.01） while TXNIP and NLRP3 mRNAs were increased （P<0.01） in the model group. Compared with the model group， the surface villi of colon tissue was more complete， the goblet cells and crypts were increased， and the muscular layer was thickened； the other indexes were reversed （P<0.01，P<0.05） in the EA group.ConclusionEA at GV16， LR3 and ST36 can reduce the abnormal accumulation of α-syn in the substania nigra and colon tissue of PD mice， alleviate the damage of intestinal barrier， regulate TXNIP/NLRP3 signaling pathway， so as to delay the occurrence and development of PD.  
        关键词：Electroacupuncture;Parkinson’s disease;α-synuclein;Thioredoxin interaction protein;Nod-like receptor 3;Intestinal barrier   
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      • “近期研究显示，针灸临床中常选用足三里作为重要腧穴处方，用于治疗胃肠功能异常相关疾病。本文系统梳理了近五年中、英文数据库中的相关基础研究，发现电针干预单穴“足三里”在改善胃肠黏膜损伤和调节胃肠动力紊乱方面有着显著作用。研究指出，电针“足三里”可能通过调节巨噬细胞、炎性反应、氧化应激和免疫微环境等多个方面来改善胃肠黏膜损伤，并通过中枢和外周信号转导及肥大细胞和Cajal间质细胞功能来调节胃肠动力紊乱。这一研究为针灸治疗胃肠功能异常相关疾病提供了科学依据，为未来的临床应用和研究提供了新的方向。”
        LIU Yun-long, LI Shan-shan, YANG Yong-rui, ZHAO Ying-hui, LI Wen-xi, HAO Jia-xin, ZHANG Bo, FAN Xiao-jing
        Vol. 48, Issue 10, Pages: 1048-1054(2023) DOI: 10.13702/j.1000-0607.20230184
        摘要：Gastrointestinal dysfunction is manifested as digestive symptoms. Clinically， Zusanli （ST36） is crucial in the acupoint prescriptions of acupuncture no matter which type of the disease is differentiated in traditional Chinese medicine， but the underlying mechanism remains to be explored. Aiming to summarize the current status of the researches in terms of ameliorating gastrointestinal mucosal damage and regulating gastrointestinal motility disorders， we systematically reviewed the basic researches on the intervention with electroacupuncture （EA） at “ST36” in treatment of the diseases related to gastrointestinal dysfunction in the past 5 years， after searching the articles from Chinese and English databases. The results suggest that EA at ST36 may regulate the local gastrointestinal inflammation， oxidative stress and immune microenvironment to relieve gastrointestinal mucosal damage and adjust gastrointestinal motility disorders by means of modulating the central and peripheral nerve signaling as well as the function of mast cells and Cajal interstitial cells.  
        关键词：Zusanli （ST36）;Gastrointestinal function;Molecular mechanism;Review   
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      • “针对帕金森病（PD）便秘问题，有研究发现便秘可能加速PD的进展，而治疗便秘则能显著改善患者生活质量，并可能延缓PD的恶化。在治疗手段方面，针灸治疗PD便秘的机制研究逐渐受到关注。然而，目前相关研究尚处于起步阶段，主要围绕神经体液因子、肠道菌群及其发酵产物、肠道炎性反应等方面进行探讨，但仍存在干预方法单一、研究思维局限以及学科间合作不足等问题。为此，本文系统地梳理了针灸治疗PD便秘的机制研究进展，旨在为未来的研究提供有价值的参考，推动针灸治疗PD便秘的深入探索和实践。”
        LI Yi-xuan, HAN Jia-hao, LI Miao-miao, ZHANG Tao
        Vol. 48, Issue 10, Pages: 1055-1061(2023) DOI: 10.13702/j.1000-0607.20230276
        摘要：Constipation may accelerate the progression of Parkinson’s disease （PD）. The quality of life in PD patients can be significantly improved when constipation is treated， hence the disease progression may be delayed. At present， the mechanism research is still at the initial stage for acupuncture in treatment of PD， focusing on neurohumoral factors， intestinal flora， bacterial fermentation products and intestinal inflammation； and there are the problems such as single intervention， thinking limitation and insufficient cooperation among disciplines. This paper systematically reviews the mechanism research progress of acupuncture for the treatment of constipation in PD so as to provide the references for the subsequent studies.  
        关键词：Parkinson’s disease;Constipation;Acupuncture-moxibustion;Progress of mechanism research   
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      • “近期研究揭示了针灸在抗炎领域的广泛应用与确切疗效，然而，其现代生物学机制研究在某种程度上忽视了炎性反应随时间变化的动态性特征。因此，体表干预体系如针灸亟需重新构建研究视角，以应对复杂的免疫调控模式。该研究从临床体表干预体系的构建出发，对比分析了古今针灸免疫调控的方法，提出了针灸等体表干预方式的有效“撬动”炎性反应的关键在于准确把握介入时机。这一发现为针灸抗炎研究提供了新的切入点与靶点，为体表干预体系在炎性反应调控中的优化与应用奠定了基础。”
        LIN Dong, GONG Meng, QI Shi-yi, ZHANG Si-jia, HUANG Ying, WU Zhi-ting
        Vol. 48, Issue 10, Pages: 1062-1068(2023) DOI: 10.13702/j.1000-0607.20220682
        摘要：Acupuncture and moxibustion therapies are widely used in the field of anti-inflammation， but the dynamic characteristics of inflammatory response with time in the modern biological mechanism research has been ignored to some extent. Therefore， the body surface intervention system represented by acupuncture-moxibustion urgently needs to rebuild the research perspective oriented to the complex immune regulation model， and then to explore the opportunity of acupuncture-moxibustion anti-inflammatory intervention according to the dynamic change process of inflammatory response. Through comparative analysis on the ancient and modern acupuncture-moxibustion immune regulation， and starting from the construction of clinical body surface intervention system， we propose that grasping the appropriate “intervention opportunity” is an important entry point that cannot be ignored to effectively “pry” inflammatory response.  
        关键词：Acupuncture and moxibustion;Timing of intervention;Inflammation response;Dynamic change   
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