最新刊期
卷 48 ， 期 11 ， 2023
- “针对阿尔茨海默病（AD）的治疗机制，研究团队进行了深入的探讨。通过构建AD大鼠模型并观察电针对其海马CA1区小胶质细胞（MG）及Janus激酶-2（JAK2）、信号转导和转录激活因子-3（STAT3）的影响，他们发现电针能显著改善AD大鼠的学习记忆能力。实验中，研究团队发现模型组大鼠存在学习记忆障碍，海马神经元受损，MG被激活，以及JAK2/STAT3信号通路异常激活等现象。而经过电针治疗后，大鼠的学习记忆能力得到明显改善，海马神经元损伤减轻，MG的激活受到抑制，同时JAK2/STAT3信号通路的异常激活也得到了有效调控。这一研究不仅为AD的治疗提供了新的思路，也为进一步探索电针治疗AD的机制奠定了基础。未来，研究团队将继续深入研究电针治疗AD的具体机制，以期为患者带来更有效的治疗方法。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on microglia （MG）， Janus kinase-2 （JAK2） and signal transducer and activator of transcription-3 （STAT3） in hippocampal CA1 region of Alzheimer’s di-sease （AD） rats， so as to explore its mechanisms in the treatment of AD.MethodsThirty-six male SD rats were randomly divided into sham operation， model and EA groups， with 12 rats in each group. The AD rat model was established by intraperitoneal injection of D-galactose combined with intrahippocampal injection of aggregated Aβ25-35. The rats in the EA group were given EA （2 Hz/20 Hz， 2 mA） at “Baihui”（GV20） and“Shenting”（GV24） for 30 min， once daily， 6 days a week for 4 weeks. Morris water maze test was used to detect the learning and memory ability and spatial exploration ability of rats. HE staining was used to observe the pathological changes of hippocampus. The ultrastructure of hippocampal neurons was observed by transmission electron microscopy. The positive expression of MG marker io-nized calcium adaptor protein （Iba-1） in hippocampus was observed by immunofluorescence staining. The expression levels of serum inflammatory factor interferon-γ （IFN-γ） and transforming growth factor beta 1 （TGF-β1） were detected by ELISA. The mRNA expression levels of JAK2， STAT3， inducible nitric oxide synthase （iNOS） and arginase-1 （Arg-1） in hippocampal CA1 region were detected by real-time quantitative PCR. The protein and phosphorylation levels of JAK2 and STAT3 in hippocampal CA1 region were detected by Western blot.ResultsCompared with the sham operation group， the escape latency of the model group was significantly prolonged （P<0.01）， and the number of crossing the original platform was significantly reduced （P<0.01）， the positive expression of Iba-1 in CA1 region， the content of serum IFN-γ， the relative mRNA expressions of JAK2， STAT3 and iNOS， and the protein and phosphorylation levels of JAK2 and STAT3 were significantly increased （P<0.01）， while the content of serum TGF-β1 and the relative expression of Arg-1 mRNA were significantly decreased （P<0.01）. Compared with the model group， the escape latency of rats in the EA group was significantly shortened （P<0.01）， the number of crossing the original platform was significantly increased （P<0.01）， the positive expression of Iba1， the content of serum IFN-γ， the mRNA expressions of JAK2， STAT3 and iNOS， and the protein and phosphorylation levels of JAK2 and STAT3 were significantly decreased （P<0.05， P<0.01）， while the content of serum TGF-β1 and the expression of Arg-1 mRNA were significantly increased （P<0.01）. Moreover， pathological and ultrastructural observation showed a reduction in the number of hippocampal neurons， changement of nuclear morphology， dilation of intercellular space， and decreased number of mitochondria in the model group； these situations were relatively milder in the EA group.ConclusionEA can improve the learning and memory function of AD rats， which may be associated with its functions in decreasing MG activities， and inhibiting the JAK2 / STAT3 signaling pathway in the hippocampus.关键词：Alzheimer’s disease;Electroacupuncture;Learning and memory ability;Microglia;Janus kinase-2;Signal transducer and activator of transcription-3;Inflammatory response145|13|0更新时间：2024-05-08
- “一项关于电针治疗血管性痴呆作用机制的研究取得了新进展。研究团队通过对比假手术组、模型组、电针组和奥拉西坦组大鼠的学习记忆功能、脑血流变化、海马CA1区微血管结构及相关蛋白表达等指标，发现电针“百会”“神庭”能够显著改善VD大鼠的学习记忆功能，并推测其机制可能与修复微血管结构、改善脑血流相关。这一研究成果为电针治疗血管性痴呆提供了新的理论支撑，也为未来探索更多有效的治疗方法开辟了新的方向。”摘要：ObjectiveTo explore the effect of electroacupuncture （EA） at “Baihui” （GV20） and “Shenting” （GV24） on the microvascular structure and related protein expression in the hippocampus of vascular dementia （VD） rat model， and to investigate the mechanism of EA in the treatment of VD.MethodsA total of 24 SD rats were randomly divided into sham operation， model， EA， and oxiracetam groups， with 6 rats in each group. Multiple cerebral infarction method was used to establish VD model. In the EA group， EA was applied to GV20 and GV24 for 30 min， once daily for 14 days. Rats in the oxiracetam group were treated with oxiracetam （50 mg/kg） by intraperitoneal injection， and the course of treatment was the same as that in the EA group. Learning and memory ability were evaluated by using Morris water maze test and new object recognition experiment. The cerebral blood flow was detected by laser doppler. The microvascular structure in the hippocampus was observed by transmission electron microscopy. The expression of vascular structure related proteins of platelet-derived growth factor receptor （PDGFR）-β，platelet endothelial cell adhesion molecule-1（CD31）， neural cadherin N-Cadherin，Zonula occludens protein-1（ZO-1） in the hippocampus were measured by Western blot.ResultsCompared with the sham operation group， the rats in the model group had a significant increase in time of first crossing the platform， a significant decrease in the number of crossing platform and the new object preference index （P<0.05）， a significant decrease in cerebral blood flow （P<0.05）， and a significant increase in the brain weight （P<0.05）. The structure boundary of pericyte and endothelial cells in the microvessels of the hippocampal CA1 area of model group was blurred， accompanied by obvious edema around the vessels and the reduction of tight junctions. The protein expression levels of PDGFR-β， CD31， N-Cadherin， ZO-1 were significantly decreased in the model group compared with those in the sham operation group （P<0.05）. Compared with the model group， the time of first crossing the platform of rats in the EA and oxiracetam group was shortened， the number of crossing platform were increased （P<0.05）， the cerebral blood flow was increased （P<0.05）， the brain weight was decreased （P<0.05）， the morphology and structure of pericyte and endothelial cells in the microvessels of hippocampal CA1 area were intact， accompanied by the increase of tight junctions. Additionally， Compared with the model group， the EA group had a significant increase in the new object preference index （P<0.05）， the protein expression levels of PDGFR-β， CD31， ZO-1 in the EA group were increased （P<0.05）， and the expression of PDGFR-β， N-Cadherin， ZO-1 in the oxiracetam group were increased （P<0.05）.ConclusionEA at GV20 and GV24 can improve the learning and memory ability of VD rats， and the mechanism may be related to the repair of microvascular structures and improvement of cerebral blood flow.关键词：Vascular dementia;Electroacupuncture;Microvascular structure;Platelet derived growth factor receptor-β;Platelet endothelial cell adhesion molecule;Neural cadherin;Zonula occludens protein-188|5|4更新时间：2024-05-08
- “最新研究揭示了电针在减轻大鼠脑缺血再灌注损伤（CIRI）中的机制。研究人员通过电针刺激“百会”和“神庭”穴位，发现这一方法能够激活Yes相关蛋白（Yap）-视神经萎缩蛋白1（OPA1）信号轴，进而促进线粒体融合功能。这一发现不仅为电针在神经保护领域的应用提供了科学依据，也为治疗CIRI等神经系统疾病提供了新的思路。实验中，研究人员通过大脑中动脉闭塞法制备了局灶性CIRI模型，并运用多种技术手段评估了神经功能缺损情况和脑梗死体积百分比。结果表明，电针组大鼠的脑梗死体积百分比和神经功能评分均显著降低，同时梗死侧大脑皮层的Yap、OPA1等关键蛋白和mRNA表达水平也得到了显著提升。这些发现进一步证实了电针通过激活Yap-OPA1信号轴来减轻CIRI损伤的有效性。此项研究不仅为我们深入理解电针的神经保护作用提供了有力支持，也为开发新的神经系统疾病治疗方法提供了重要启示。未来，我们有望看到更多基于电针治疗技术的创新应用在神经系统疾病领域取得突破。”摘要：ObjectiveTo investigate the mechanism of electroacupuncture （EA） in alleviating cerebral ische-mia injury by activating the Yap-OPA1 signaling axis.MethodsA total of 48 male SD rats were used in the present study. The focal CIRI model was established by occlusion of the middle cerebral artery and reperfusion （MCAO/R）， followed by dividing the CIRI rats into model group， EA group and EA+Ver （Verteporfin， Yap antagonist） group （n=12 in each group）. And another 12 normal rats were used as the sham operation group. For rats of the EA group， EA （4 Hz/20 Hz， 0.5 mA） was applied to “Baihui”（GV20） and “Shenting”（GV24） for 20 min， once daily for 7 days. The neurological deficit score （0 to 4 points） was given according to Longa's method. The infarct volume of rats in each group was assessed by TTC method， and the expression levels of Yes associated protein （Yap）， Optic atrophy protein 1 （OPA1）， mitofusin 1 （Mfn1）， mitofusin 2 （Mfn2） proteins and mRNAs in cerebral cortex of infarcted side， as well as Bax （proapoptotic factor） and Bcl-1 （anti-apoptotic protein） proteins were detected by Westernblot， and real-time PCR， and the immunoactivity of Yap and OPA1 was detected by immunofluorescent staining.ResultsAfter modeling， the infarct volume， neurological deficit score and the expression of Bax were significantly increased （P<0.01）， while the mRNA and protein expressions of Yap， OPA1， Mfn2， Mfn1， and Bcl-2 were significantly down-regulated in the model group relevant to the sham operation group （P<0.01，P<0.05）. Compared with the model group， the neurological deficit score， infarct volume and the expression of Bax were significantly decreased （P<0.01）， while the expression levels of Yap， OPA1， Mfn2， Mfn1 proteins and mRNAs and Bcl-2 protein， Yap and OPA1 immunofluorescence intensify were considerably up-regulated in the EA group （P<0.01，P<0.05）. Following administration of Ver， the effects of EA in down-regulating the neurological score， infarct volume， and Bax expression and up-regulating the expressions of Yap， OPA1， Mfn1， Mfn2 proteins and mRNAs and Yap and OPA1 immunofluorescence intensify were eliminated.ConclusionEA of GV20 and GV24 can improve the neurological function in rats with CIRI， which may be associated with its functions in activating mitochondrial fusion function and up-regulating Yap-OPA1 signaling axis.关键词：Cerebral ischemia-reperfusion injury;Electroacupuncture;Yap;OPA1;Mitochondrial fusion76|4|0更新时间：2024-05-08
- “火针治疗奥沙利铂诱导的神经病理性疼痛大鼠的机制研究取得新进展。研究通过构建NP大鼠模型，发现火针治疗能显著提高大鼠的机械痛阈，降低血清炎性因子水平，减轻组织病理损伤。这一作用可能与抑制肥大细胞活化，下调穴区局部TPS、TRPV1和PAR2蛋白的表达有关。该研究不仅为火针治疗神经病理性疼痛提供了实验依据，也为进一步探讨火针镇痛机制奠定了基础。”摘要：ObjectiveThis study aims to explore the analgesic mechanism of fire needle on peripheral sensitization in rats with neuropathic pain（NP） induced by oxaliplatin， so as to investigate its mechanism in improving peri-pheral sensitization.MethodsMale SD rats aged 8 weeks were randomly divided into 4 groups： normal group（n=6）， model group（n=6）， fire needle group（n=6）， and medication group（n=6）. NP rat model was established by intraperitoneal injection of oxaliplatin（4 mg/kg） on days 1， 2， 8， 9， 15， 16， 22， and 23. For rats in the fire needle group， fire needle treatment was performed at the “Jiaji”（EX-B2） acupoints of the L4—L6 segments on days 24， 26， and 28， ie. 1 day， 3 and 5 days after modeling. The medication group received intraperitoneal injection of pregabalin（100 mg/kg）. Mechanical pain thresholds of the rats were measured before modeling， after modeling and intervention. Serum contents of tumor necrosis factor-α（TNF-α）， interleukin-6（IL-6） and chemokine ligand 12（CXCL12） were detected by ELISA. Skin histopathology changes in the acupoint area were observed using HE staining. The number of mast cells in the skin of the acupoints was observed using toluidine blue staining. Immunohistochemical staining was performed to detect the postive expressions of transient receptor potential vanilloid 1（TRPV1）， protease-activated receptor 2（PAR2） and tryptase（TPS） in the skin of the acupoint area. Western blot was used to detect the protein expressions of TRPV1 and PAR2 in the dorsal root ganglia（DRG）.ResultsCompared with the normal group， the model group had decreased paw withdrawal threshold（PWT） after modeling（P<0.05）， increased serum contents of IL-6， TNF-α， and CXCL12（P<0.05）， increased number of mast cells in the acupoint area（P<0.05）， and increased positive protein expressions of TPS， TRPV1， and PAR2 in the skin of the acupoint area（P<0.05）. Compared with the model group， the fire needle group and medication group had increased PWT after intervention（P<0.05）， decreased serum contents of IL-6， TNF-α， and CXCL12， and postive protein expressions of TPS， TRPV1， and PAR2 in the skin of the acupoint area（P<0.05）； while the medication group had decreased protein expressions of TRPV1 and PAR2 in DRG（P<0.05）. HE staining showed thickened epidermis， disordered cellular arrangement， significant intercellular edema， and inflammatory cell infiltration in the model group. In the medication and fire needle groups， the epidermis was thinner， cellular arrangement was clearer， and the extent of tissue edema and inflammatory cell infiltration was reduced compared to the model group.ConclusionFire needle can improve mechanical pain threshold and reduce the contents of peripheral inflammatory factors in rats with oxaliplatin-induced NP. This effect may be related to the inhibition of mast cell activation and the inhibition of TPS， TRPV1 and PAR2 protein expressions， in the local areas of acupoints.关键词：Neuropathic pain;Fire needle;Transient receptor potential vanilloid 1/protease-activated receptor 2;Oxaliplatin;Mast cells;Neuroinflammation88|6|1更新时间：2024-05-08
- “针对类风湿性关节炎（RA）的治疗机制，研究人员通过针刺“阳陵泉”“足三里”“悬钟”的方法，对CIA大鼠进行了深入的探索。该研究旨在观察针刺对这些大鼠关节炎指数、关节滑膜病理形态、血清免疫炎性因子以及相关蛋白表达的影响。实验结果显示，针刺治疗能有效降低CIA大鼠的关节炎指数，减轻关节肿胀程度，并调节血清中TNF-α、IL-6及IL-17等炎性因子的表达。进一步的研究还发现，针刺能影响关节滑膜组织中mt-p53、NF-κB p65、PPARγ等蛋白及mRNA的表达水平，为理解针刺治疗RA的机制提供了新的视角。该研究成果为类风湿性关节炎的治疗提供了新的思路和方法，也为针灸治疗在现代医学中的应用提供了有力支持。这一发现不仅有助于深化我们对针灸作用机制的理解，也为针灸在RA治疗中的临床应用提供了科学依据。”摘要：ObjectiveTo observe the effect of acupuncture stimulation of “Yanglingquan”（GB34）， “Zusanli”（ST36） and “Xuanzhong” （GB39） on arthritis index （AI）， joint synovial membrane pathology， serum-related immunoinflammatory factors， and expressions of tumor suppressor gene mt-p53， nuclear factor kappa B （NF-κB） and peroxisome proliferator activated receptor gamma （PPARγ） in knee joint synovial tissue of rats with type Ⅱ collagen-induced arthritis （CIA）， so as to explore its possible mechanisms underlying improvement of rheumatoid arthritis （RA）.MethodsMale SD rats were used in the present study. The CIA model was established by subcutaneous injection of collagen emulsion （200 μL/rat） in the tail root region on the first day and repeat （100 μL/rat） once on the 9th day. Eighteen successful CIA rats were randomized into model， medication and acupuncture groups， with 6 rats in each group. Other 6 normal rats were used as the normal control group. For rats of the medication group， leflunomide （1.9 mg/kg） was administrated by gavage， once a day， and for rats of the acupuncture group， manual acupuncture stimulation was applied to bilateral GB34， ST36， GB39 for 30 min， once a day， for 12 weeks. The arthritis index （AI） score （0—4 points） was evaluated once every week. The contents of IL-6， IL-17 and TNF-α in the serum were determined by ELISA. Histopathological changes of the ankle joint were observed by H.E. staining. The protein and mRNA expression levels of mt-p53， NF-κB p65， and PPARγ in the knee joint synovial tissue were determined by Western blot and quantitative real time PCR， separately.ResultsCompared with the normal control group， the AI scores at different time-points after modeling， contents of serum TNF-α， IL-6 and IL-17， expression levels of mt-p53， NF-κB p65， PPARγ proteins and mRNAs were significantly increased in the model group （P<0.01， P<0.05）. In comparison with the model group， the AI scores at the 10th week in the medication group and at the 3rd， 9th and 10th week in the acupuncture group， contents of serum TNF-α， IL-6 and IL-17， and the expression levels of mt-p53 and NF-κB p65 proteins in both medication and acupuncture groups， as well as mt-p53 and NF-κB p65 mRNAs in the medication group were apparently decreased （P<0.01， P<0.05）， while the expression levels of PPARγ protein in both medication and acupuncture group and PPARγ mRNA in the medication group were significantly up-regulated （P<0.05， P<0.01）. No significant differences were found between the acupuncture and medication groups in down-regulating the AI score and serum TNF-α， IL-6 and IL-17 contents. The effect of acupuncture was weaker than that of medication in down-regulating the expression of mt-p53 and NF-κB p65 proteins and mRNAs and in up-regulating PPARγ mRNA （P<0.01）. H.E. results showed ankle cartilage hyperplasia， reduced joint cavity， mild fibroproliferation and inflammatory cell infiltration in the surrounding soft tissue of the ankle joint in rats of the model group， which was milder in both medication and acupuncture groups.ConclusionAcupuncture stimulation can improve the degree of joint inflammation and swelling in CIA rats， which may be related to its effects in inhibiting the overexpression of immunoinflammatory factors in serum and regulating expression of mt-p53， NF-κB p65， PPARγ mRNAs and proteins in the synovial tissue.关键词：Rheumatoid arthritis;Acupuncture;Inflammatory factor;PPARγ;NF-kB;Mutant P5376|15|1更新时间：2024-05-08
- “一项关于艾灸对佐剂性关节炎大鼠作用机制的研究取得了重要进展。研究团队通过构建AA大鼠模型，观察了艾灸对大鼠关节肿胀度、关节炎指数的影响，并深入探讨了艾灸对类风湿关节炎的作用机制。实验结果显示，与正常组相比，模型组大鼠关节肿胀度和关节炎指数显著升高，滑膜组织中的NLRP3、ASC、Caspase-1、TNF-α和IL-1β等炎性蛋白表达水平也显著升高。而在艾灸治疗后，大鼠关节肿胀度和关节炎指数明显降低，滑膜组织中的炎性蛋白表达水平也相应下降。此外，艾灸组大鼠关节滑膜细胞的超微结构损伤程度也明显减轻。这一发现表明，艾灸能够明显改善AA大鼠的关节炎症反应，其机制可能与艾灸抑制NLRP3/Caspase-1/IL-1β炎性小体信号通路有关。这一研究成果为深入理解艾灸治疗类风湿关节炎的作用机制提供了新的思路和方法，为针灸疗法的应用和发展提供了新的科学依据。”摘要：ObjectiveTo observe the effect of moxibustion on activities of NOD-like receptor family protein 3 （NLRP3）/cysteine aspartic acid specific protease-1 （Caspase-1）/interleukin-1β （IL-1β） signaling pathway in rats with adjuvant arthritis （AA）， so as to explore its mechanisms underlying improvement of rheumatoid arthritis （RA）. Me-thods Thirty male Wistar rats were randomly divided into normal control， AA model and moxibustion groups， with 10 rats in each group. The AA model was replicated by raising in wind， cold and damp environment combined with complete Freund’s adjuvant injection. In the moxibustion group， moxibustion was applied to bilateral “Shenshu” （BL23） and “Zusanli”（ST36） for 20 min each time， once daily for 21 days. Changes of joint swelling degree （JSD） and arthritis index （AI） in each group were observed. The ultrastructural changes of synovial cells in each group were observed by transmission electron microscopy. The protein expression levels of NLRP3， apoptosis-associated speck-like protein （ASC）， Caspase-1， tumor necrosis factor-α （TNF-α） and IL-1β in the synovial tissues of the knee joint were measured by Western blot.ResultsCompared with the normal control group， JSD， AI and the protein expressions of NLRP3， ASC， Caspase-1， TNF-α and IL-1β in the synovial tissues were significantly increased （P<0.01） in the model group. In comparison with the model group， JSD， AI and the protein expression levels of NLRP3， ASC， Caspase-1， TNF-α and IL-1β were significantly decreased （P<0.01） in the moxibustion group. Results of transmission electron microscope showed an irregular and vague nuclear membrane of synovial cells， and unclear mitochondrial membrane boundary with sparse， swelling crests in the model group， which was relatively milder in the damage degree in the moxibustion group.ConclusionMoxibustion can relieve the inflammatory response in the synovial membrane of AA rats， which may be related to its function in down-regulating synovial NLRP3/Caspase-1/IL-1β inflammatory signaling.关键词：Adjuvant arthritis;Moxibustion;NLRP3/Caspase-1/IL-1β signaling pathway51|4|2更新时间：2024-05-08
- “在膝关节骨关节炎（KOA）的早期治疗领域，研究团队利用核磁共振（MR）T2 mapping成像技术，深入探讨了温针灸对家兔软骨退变修复的作用。实验结果表明，温针灸能显著降低早期KOA兔关节软骨中的T2值，这与降低软骨细胞外基质中基质金属蛋白酶（MMP）-1、MMP-13蛋白表达水平密切相关。这一发现不仅证实了T2值在评估温针灸修复KOA兔早期软骨退变中的价值，也为该领域提供了新的研究方向和解决方案。这一研究不仅有助于我们更深入地理解KOA的发病机制，也为早期KOA的治疗提供了新的思路和方法。”摘要：ObjectiveTo observe the application value of MR T2 mapping for evaluating the effect of warm acupuncture-moxibustion on articular cartilage degeneration， and to observe the relationship between T2 value and expression of matrix metalloproteinases （MMP）-1 and MMP-13 of chondrocytes in rabbits with early knee osteoarthritis （KOA）.MethodsThirty male New Zealand rabbits were randomly divided into blank control， KOA model and warm acupuncture-moxibustion groups， with 10 rabbits in each group. The early KOA model was established by right hind limb tubular plaster extension fixation method for 2 weeks. The rabbits of the warm acupuncture-moxibustion group received warm acupuncture-moxibustion stimulation at “Heding”（EX-LE2），“Neixiyan”（EX-LE4），“Waixiyan” （EX-LE5） and“Zusanli”（ST36） on the right hind limb for 15 min， once a day for 2 weeks. After intervention， MR T2 mapping of the right knee joint was performed in each group. The H.E. staining was used to evaluate the histopathological changes of cartilage， followed by giving a score according to the standards of Mankin scoring. The TUNEL method was used to analyze the apoptosis state of chondrocytes， and the positive expressions of MMP-1 and MMP-13 in the articular cartilage were detected by immunohistochemical staining.ResultsCompared with the blank control group， the Mankin score， chondrocyte apoptosis index， T2 value and the positive expressions of MMP-1 and MMP-13 in the cartilage tissue were significantly increased in the model group （P<0.01）. Compared with the model group， the Mankin score， chondrocyte apoptosis index， T2 value and the positive expressions of MMP-1 and MMP-13 in the cartilage tissue were markedly decreased in the warm acupuncture-moxibustion group （P<0.01）. The T2 value was positively correlated with the expression levels of MMP-1 and MMP-13 （P<0.01）. H.E. staining showed disordered arrangement of chondrocytes and thinner cartilage layer in the model group， and a clear and relative ordered arrangement of chondrocyte in the warm acupuncture-moxibustion group.ConclusionWarm acupuncture-moxibustion can reduce the T2 value of articular cartilage in early KOA rabbits， which is positively correlated with the decreased expression of MMP-1 and MMP-13 in the extracellular matrix of cartilage. The MR T2 mapping has certain value in evaluating the effect of warm acupuncture-moxibustion on KOA rabbits with early cartilage degeneration.关键词：Knee osteoarthritis;Warm acupuncture-moxibustion;Cartilage degeneration;MR T2 mapping55|9|1更新时间：2024-05-08
- “一项关于电针对肥胖胰岛素抵抗大鼠小肠沉默信息调节因子1（SIRT1）/Toll样受体4（TLR4）炎性信号通路影响的研究取得新进展。该研究通过观察电针对肥胖胰岛素抵抗大鼠的影响，探讨了电针改善肥胖胰岛素抵抗的机制。研究中，Wistar大鼠被随机分为正常组、模型组、电针组和电针联合抑制剂组，每组10只。通过高脂饮食饲养8周建立肥胖胰岛素抵抗大鼠模型。电针组大鼠接受了中脘、关元、足三里、丰隆等穴位的电针治疗，共8周。同时，电针联合抑制剂组大鼠在电针治疗的基础上，还接受了SIRT1抑制剂Sirtinol的的尾静脉注射。研究结果显示，与正常组相比，模型组大鼠体质量显著升高，葡萄糖输注速率显著降低，血清炎性因子含量升高，小肠黏膜损伤严重，SIRT1蛋白表达降低，TLR4蛋白表达升高。经过电针干预后，电针组和电针联合抑制剂组大鼠体质量降低，葡萄糖输注速率升高，血清炎性因子含量降低，小肠黏膜损伤减轻，TLR4蛋白表达降低，SIRT1蛋白表达升高。而在电针联合抑制剂组中，这些指标的改善程度不如单纯电针组。这项研究表明，电针可以改善肥胖胰岛素抵抗大鼠的体质量和胰岛素敏感性，其机制可能与调控小肠组织中SIRT1/TLR4炎性信号通路有关。这为肥胖胰岛素抵抗的治疗提供了新的思路和方法。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） in obese rats with insulin resistance （IR） through regulating intestinal silent information regulator 1 （SIRT1）/Toll-like receptor 4 （TLR4） signaling pathway， so as to explore the underlying mechanism of EA in improving obesity-induced IR.MethodsA total of 40 Wistar rats were randomly divided into 4 groups， i.e. normal group， model group， EA group and EA combined with inhibitor group， with 10 rats in each group. The obesity-induced IR model was induced by feeding high-fat diet for 8 weeks. EA （2 Hz， 1mA） was applied at “Zhongwan”（CV12）， “Guanyuan”（CV4）， “Zusanli”（ST36） and “Fenglong” （ST40） for 10 min， 3 times a week for 8 weeks in both EA and EA combined with inhibitor groups. Sirtinol， an inhibitor of SIRT1 was injected into the tail vein （1 mg/kg）， 3 times a week for 8 weeks in EA combined with inhibitor group. The body weight， glucose infusion rate （GIR） of rats in each group were recorded. The contents of serum C-reactive protein （CRP）， tumor necrosis factor-α （TNF-α）， interleukin-6 （IL-6） and lipopolysaccharide （LPS） were detected by ELISA. Mucosal morphological changes in the small intestine was observed by HE staining and was graded using Chiu’s score. The protein relative expression levels of SIRT1 and TLR4 and the co-labeling of SIRT1 with TLR4 in the small intestine was detected by Western blot and double immunofluorescence staining， separately.ResultsCompared with the normal group， the body weight， serum contents of CRP， TNF-α， IL-6， LPS， Chiu’s score， TLR4 protein relative expression level and percentage of TLR4 positive expression area were increased （P<0.01， P<0.05）， while the GIR， SIRT1 protein expression， percentage of SIRT1 positive expression area and SIRT1/TLR4 were decreased （P<0.01） in the model group. The pathological injury of small intestine mucosa was severe， accompanied with inflammatory cell infiltration in the model group. Following interventions， the body weight， serum contents of CRP， TNF-α and LPS， Chiu’s score， TLR4 protein relative expression level and percentage of TLR4 positive expression area were decreased（P<0.01， P<0.05）， and the GIR was increased （P<0.01）， the pathological injury and inflammatory cell infiltration of small intestine mucosa were reduced in both EA and EA combined with inhibitor groups in contrast to the model group. Compared with the model group， the serum IL-6 content was significantly decreased （P<0.01）， and the SIRT1 protein relative expression level and percentage of positive expression area， SIRT1/TLR4 were increased （P<0.01， P<0.05） in the EA group. Compared with the EA group， EA combined with inhibitor group showed the body weight， serum CRP， IL-6， LPS， Chiu’s score， TLR4 protein relative expression level and TLR4 positive expression area were increased （P<0.01， P<0.05）， and the GIR level ， SIRT1 relative expression level， SIRT1/TLR4 ratio were decreased （P<0.05，P<0.01）.ConclusionEA can reduce the body weight and ameliorate peripheral insulin sensitivity in IR obese rats，which may be related with its function in regulating intestinal SIRT1/TLR4 signaling pathway to reduce inflammatory response.关键词：Obesity;Insulin resistance;Electroacupuncture;Silent information regulator 1;Toll-like receptor 464|5|2更新时间：2024-05-08
- “在中医针灸治疗领域，近日有论文探讨了艾灸与针刺在治疗腹泻型肠易激综合征（IBS-D）模型大鼠上的差异。研究采用“标本配穴”的方法，对比了艾灸和针刺对大鼠的治疗效果。结果显示，与模型组相比，艾灸组和针刺组的大鼠稀便率降低，旷场实验活动增加，疼痛阈值和容量阈值升高，心电图的心率变异性指标改善，血清炎症因子水平降低。进一步比较发现，艾灸组在多个指标上的改善效果优于针刺组。这表明，“标本配穴”的艾灸方法在治疗IBS-D上可能具有更好的效果，为中医针灸治疗IBS-D提供了新的思路和方法。”摘要：ObjectiveTo compare the effects of moxibustion and acupuncture of combined “Biao-Ben” acupoints （Biao indicates pathogenic factors of disease， Ben refers to body constitution） on a rat model of irritable bowel syndrome with diarrhea （IBS-D）.MethodsForty female SD rats were randomly divided into 4 groups： normal group， model group， moxibustion group， and acupuncture group， with 10 rats in each group. The IBS-D rat model was established by administering acute-chronic stress combined with folium sennae gavage for 28 days. Rats in the moxibustion group received moxibustion at bilateral “Zusanli”（ST36）， “Guanyuan”（CV4）， and “Neiguan”（PC6）， while those in the acupuncture group received acupuncture at the same acupoints， both for 15 min every time， once a day. The treatments were administered for 21 days. The loose stool rate was observed. Colonic pain threshold and colonic distension threshold were measured by a self-made balloon catheter. Total distance traveled and grid crossing numbers were observed by open field test. Heart rate variability（HRV） time domain indexes SDANN and PNN50 were acguired by using electrophysiological recorder. Histopathological changes in the colon tissue were observed after HE staining. Contents of interleukin-6（IL-6）， IL-8， and tumor necrosis factor-alpha（TNF-α） in serum were detected by ELISA.ResultsCompared with the normal group， rats in the model group showed increased loose stool rate（P<0.05）， decreased pain threshold and distension threshold（P<0.05）， reduced total distance traveled and grid crossing numbers in the open field test（P<0.05）， decreased HRV time domain indexes SDANN and PNN50（P<0.01， P<0.05）， and elevated levels of serum IL-6， IL-8， and TNF-α contents（P<0.05）. Compared with the model group， the moxibustion group and acupuncture group showed decreased loose stool rate（P<0.05）， increased total distance traveled and grid crossing numbers in the open field test（P<0.05）， increased pain threshold and distension threshold（P<0.05）， increased SDANN and PNN50 （P<0.05）， and decreased levels of serum IL-6， IL-8， and TNF-α contents（P<0.05）. Compared with the acupuncture group， the moxibustion group showed further decreased loose stool rate（P<0.05）， increased total distance traveled and grid crossing numbers in the open field test（P<0.05）， increased pain threshold and distension threshold（P<0.05）， increased SDANN and PNN50（P<0.05）， and decreased levels of serum IL-6， IL-8， and TNF-α contents（P<0.05）. No significant pathological changes were observed in the colon tissue of rats in each group.ConclusionMoxibustion of combined “Biao-Ben” acupoints is more effective in regulating HRV and serum IL-6， IL-8， and TNF-α contents in the IBS-D rat model. Based on the combined “Biao-Ben” acupoints method， moxibustion has better therapeutic effects on IBS-D than acupuncture.关键词：Diarrhea-predominant irritable bowel syndrome;Combined “Biao-ben” acupoints;Moxibustion;Acupuncture;Heart rate variability;Inflammatory cytokines51|4|2更新时间：2024-05-08
- “一项关于针刺治疗腹泻型肠易激综合征（IBS-D）的研究取得了重要进展。研究团队通过精心设计的实验，发现针刺对IBS-D大鼠的脑肠肽及相关炎性因子表达产生了显著影响。这一发现不仅揭示了针刺治疗IBS-D的作用机制，还为未来的临床应用提供了理论基础。在研究中，研究者们首先通过冰醋酸灌肠和束缚应激的方式成功建立了IBS-D大鼠模型。随后，他们分别给予药物和针刺治疗，并详细观察了各组大鼠的生理指标和生化表达情况。结果显示，针刺组大鼠在一般状态、情绪状态、炎性反应和相关脑肠肽表达等方面均表现出显著改善。与模型组相比，针刺组大鼠的体质量、旷场实验行走距离及直立次数均显著增加，血清IL-10含量也显著升高。同时，针刺组大鼠的粪便粒数及结肠组织MC数量明显减少，血清TNF-α含量和下丘脑、结肠组织中CGRP mRNA表达水平也显著降低。这些结果表明，针刺能够有效改善IBS-D大鼠的症状和炎性反应，并调控相关脑肠肽的表达。这项研究不仅证实了针刺在IBS-D治疗中的积极作用，还为我们揭示了其潜在的作用机制。未来，随着研究的深入，针刺治疗IBS-D的方法有望在临床中得到更广泛的应用，为广大患者带来福音。”摘要：ObjectiveTo observe the effect of acupuncture on the expressions of neuropeptides and related inflammatory factors in rats with diarrhea-predominant irritable bowel syndrome（IBS-D）， so as to explore the mechanism of acupuncture in the treatment of IBS-D.MethodsMale Wistar rats were randomly divided into blank group， model group， medication group， and acupuncture group， with 6 rats in each group. Except for the blank group， the other groups were subjected to 14-day “acetic acid enema + restraint stress” to establish the IBS-D rat model. After successful modeling， the medication group received gavage of pinaverium bromide（15 mg/kg） once a day， and the acupuncture group received acupuncture at “Baihui”（GV20） and bilateral “Tianshu”（ST25）， “Shangjuxu”（ST37）， “Zusanli”（ST36）， and “Taichong”（LR3） for 20 min every day， both groups were treated continuously for 14 days. The general state of the rats in each group was observed， and the body weight of the rats was measured. The open-field experiment was conducted to measure the horizontal and vertical movements， and the number of fecal pellets of rats. The histopathological morphology of hypothalamus and colon of rats was observed by HE staining. Toluidine blue staining was used to observe and count the mast cells（MCs） in the colon tissue of rats. ELISA was used to detect the serum contents of tumor necrosis factor-α（TNF-α） and interleukin（IL）-10. Real-time fluorescence quantitative PCR was performed to detect the mRNA expressions of calcitonin gene-related peptide（CGRP） in the hypothalamus and colon tissue. Western blot was used to detect the expressions of corticotropin-releasing factor（CRF） in the hypothalamus and colon tissue.ResultsHE staining showed that there was inflammatory cell infiltration in the lamina propria of colon in the model group， and it was reduced in the other groups. Compared with the blank group， the model group showed significantly decreased body weight， decreased walking distance and upright times in open field experiment， decreased serum IL-10 contents（P<0.05， P<0.01）， increased fecal pellet number （P<0.01）， increased MC number in the colon tissue， serum TNF-α contents， and CGRP mRNA expressions and CRF expressions in the hypothalamus and colon tissue（P<0.01）. Compared with the model group， both medication and acupuncture groups showed significantly increased body weight， walking distance and upright times in the open-field experiment， and serum IL-10 contents（P<0.01，P<0.05）， significantly decreased fecal pellet number （P<0.05）， significantly decreased MC number in the colon tissue， serum TNF-α contents， and CGRP mRNA expressions in the hypothalamus and colon tissue（P<0.01）； at the same time， the acupuncture group showed significantly decreased CRF expressions in the hypothalamus and colon tissue（P<0.01，P<0.05）. There was no significant difference in the above indicators between the medication group and the acupuncture group.ConclusionAcupuncture can improve the general and emotional state， inflammatory response， and neuropeptide expression in rats with IBS-D， and alleviate the symptoms of IBS-D， which may be related to the regulation of neuropeptides and inflammatory factors levels.关键词：Diarrhea-predominant irritable bowel syndrome;Acupuncture;Neuropeptide;Inflammatory response;Mast cells81|9|3更新时间：2024-05-08
- “一项关于紧张性头痛（TTH）治疗的研究取得重要进展。该研究采用火针点刺颅周压痛点联合毫针安神刺法，有效改善了TTH患者的临床症状，并显著减轻了颅周肌肉压痛。研究中，试验组患者在接受火针和毫针治疗后，头痛发作天数减少，头痛程度减轻，颅周肌肉压痛点数量减少，压痛阈值升高。这些改善效果均优于对照组，显示出火针点刺颅周压痛点联合毫针安神刺法的优势。研究还发现，头痛发作的减少和减轻程度与颅周肌肉压痛点的减少数量和压痛阈值的升高程度呈正相关，这进一步证实了颅周压痛与TTH发病之间的紧密关系。这项研究不仅为TTH的治疗提供了新的有效方法，也为我们深入理解TTH的发病机制和颅周压痛的影响提供了重要依据。火针点刺颅周压痛点联合毫针安神刺法为紧张性头痛的治疗开辟了新方向，有望为更多患者带来福音。”摘要：ObjectiveTo observe the curative effect of fire needling pricking pericranial tender points combined with filiform needling on tension-type headache （TTH） and its effect on pericranial muscle tenderness， and explore the correlation between changes of headache symptoms and pericranial muscle tenderness in TTH， to analyze the influence of pericranial muscle tenderness on TTH.MethodsA total of 41 TTH patients in the treatment group and 38 TTH patients in the control group completed the study. The patients in the treatment group were treated with fire needling at pericranial tender points combined with filiform needling at Baihui （GV20）， Sishencong （EX-HN1）， Shenting （GV24）， Touwei （ST8） and Fengchi （GB20）. The patients in the control group were only treated with the same filiform needling as the treatment group. Patients in the two groups were treated twice a week for 8 weeks. Before and after treatment， the days of headache onset， the number and distribution of pericranial muscle tender points were recorded， the degree of headache was evaluated by visual analogue scale and the threshold of pericranial muscle tender points were measured. The correlations between the changes of the days and degree of headache onset and the changes of the number and threshold of pericranial muscle tender points were analyzed. The effective rates in the two groups were calculated.ResultsCompared with those before treatment， the days of headache onset and the degree of headache were decreased （P<0.05） in the two groups； the number of pericranial muscle tender points was decreased （P<0.05） and the tenderness threshold was increased （P<0.05） in the treatment group. After treatment， compared with the control group， the days of headache onset， the degree of headache， and the number of pericranial muscle tender points were decreased （P<0.05）， and the tenderness threshold was increased （P<0.05） in the treatment group. The decrease of the days and degree of headache was positively correlated with the decrease of number and the increase of tenderness threshold of pericranial muscle tender points （P<0.05）. The effective rate in the treatment group was 87.80% （36/41）， which was higher than 57.89% （22/38） in the control group （P<0.05）. The most common anatomic location of tender points in baseline was superior trapezius muscle， followed by sternocleidomastoid muscle， superior nuchal line， temporal muscle， masseter muscle， etc.ConclusionThe fire needling at the pericranial muscle tender points combined with filiform needling on TTH patients can significantly improve the clinical symptoms and reduce the pericranial muscle tenderness. The pericranial muscle tenderness is an important factor in the pathogenesis of TTH.关键词：Fire needle;Tension-type headache;Pericranial muscle tenderness53|5|1更新时间：2024-05-08
- “一项关于针刺防治脓毒症的研究取得了重要进展。脓毒症是一种严重威胁人类生命健康的疾病，目前临床上主要依赖支持治疗，缺乏特异性治疗方法。然而，针刺作为一种传统中医疗法，在防治脓毒症方面展现出重要的临床意义。研究团队系统检索了中国期刊全文数据库和PubMed数据库，全面归纳了针刺防治脓毒症的临床试验和动物实验文献。结果显示，针刺通过抑制全身炎性反应、减轻氧化应激、调节免疫系统和抗细胞死亡等多种机制，发挥多器官保护作用。这些机制涉及复杂的信号通路、免疫细胞和细胞因子。这项研究为脓毒症的治疗提供了新的视角和解决方案，证实了针刺在防治脓毒症中的重要作用。未来，随着研究的深入，针刺疗法有望在脓毒症的临床治疗中发挥更大的作用。”摘要：Sepsis is a major disease that threatens human life and health. Clinically， it is mainly based on supportive treatment and lacks specific treatment methods. Acupuncture has important clinical significance in the prevention and treatment of sepsis. In the present paper， we systematically searched CNKI and PubMed databases， included the clinical trials and animal experiments on the prevention and treatment of sepsis with acupuncture， summarized the clinical efficacy and the mechanism of acupuncture. Results indicate that the role of acupuncture therapies in improving sepsis involves inhibiting systemic inflammatory response， alleviating oxidative stress， regulating immune system， and resisting cell apoptosis， thus having a protective effect on multiple organs. The mechanism involves multiple signaling pathways and related factors.关键词：Sepsis;Acupuncture;Inflammation;Oxidative stress;Immune regulation;Cell death;Organ dysfunction82|5|0更新时间：2024-05-08
- “科技新闻播报：针对灸法领域，目前国内外尚未形成系统的、全流程的标准体系，这在一定程度上限制了行业的创新与技术进步。通过回顾研究现状，专家发现，现有的灸法标准主要集中在技术标准方面，如艾灸、热敏灸、督灸、麦粒灸、药泥灸等。但在灸材、灸具的标准化、装备建设、艾烟净化以及管理和工作标准等方面，仍存在较多空白。为了推动灸法领域的标准化发展，专家在阐述灸法标准体系框架的基础上，提出了进一步深入研究的方向。建议从技术分层操作、灸法选材、新型灸具研发、人才培养和装备管理等方面，深入开展灸法标准化的研究工作。这一研究成果将为灸法行业的规范化、标准化提供有力支撑，为行业的健康发展注入新的动力。”摘要：There is no systematic and whole-process system for moxibustion standard development at home and abroad， which restricts the industry innovation and technological progress to a certain extent. The paper reviews the study status and finds that the technical standard is dominant in moxibustion standard development currently， represented by conventional moxibustion， heat-sensitive moxibustion， moxibustion on the Governor Vessel， moxibustion with seed-size moxa cone and herb-isolated moxibustion， etc. There are many gaps in the standards development of moxibustion material and device， equipment building， moxa smoke purification， and management and job. On the basis of explaining the standard framework of moxibustion， it is suggested that the moxibustion standardization should be deepened in the aspects of hierarchical technical operation， material selection， research and development of new devices， personnel training and equipment management.关键词：Moxibustion;Standard system;Standardization;Status;Approaches62|6|0更新时间：2024-05-08
- “一项针对近30年针灸治疗失语症的中文文献的研究分析取得了重要进展。研究团队利用知识图谱技术，对中国知网、万方数据知识服务平台和维普期刊全文数据库中的相关文献进行了深入的数据挖掘和可视化分析。研究结果显示，针灸治疗失语症领域的研究热点逐渐明确，主要聚焦于运动性失语、癔病性失语等五种失语症类型，同时头针、舌针等刺灸法以及特定的穴位也被频繁提及。近五年来，重复经颅刺激疗法、语言康复训练等新技术也成为了研究焦点。这些发现为针灸治疗失语症的研究和应用提供了新的视角和思路，有望推动该领域的进一步发展。”摘要：ObjectiveTo investigate the hot topics in acupuncture-moxibustion research for treatment of aphasia and explore the current situation and trend of technology transformation in this field through analyzing the relevant Chinese literatures in recent 30 years by means of knowledge graph technology.MethodsCiteSpace 6.1.R 2 and VOSviewer V1.6.16 software were used to collate the data， draw knowledge graphs and conduct visual analysis of the literatures related to acupuncture-moxibustion treatment of aphasia， searched from CNKI， WanFang and VIP databases.The time line view and strongest bursts of keywords were formed in the field of acupuncture-moxibustion treatment for aphasia. The treatment-based keyword networks were visualized.ResultsA total of 773 Chinese articles were included. Through visual analysis of the co-occurrence networks， the top 10 high-frequency overall keywords and the top 10 clusters of overall keywords were listed. The top 5 high-frequency aphasia categories were Broca aphasia， hysterical aphasia， transcortical motor aphasia， nominal aphasia and sensory aphasia. Regarding the keywords of the techniques of acupuncture-moxibustion， the occurrence frequencies of scalp acupuncture，tongue acupuncture，body acupuncture and electroacupuncture were ≥ 10 times.The occurrence frequencies of 16 acupoints were ≥25 times. After collation and cluster analysis of acupoints and techniques of acupuncture-moxibustion， 7 keyword clusters of “acupuncture techniques-acupoints” were obtained. The time line view showed that the strongest burst of keywords were transcranial magnatic stimulation， language rehabilitation training， acupuncture-medicine therapy and stroke， etc. in the recent 5 years.ConclusionAcupuncture-moxibustion displays its unique advantage in treatment of aphasia. With the deepening of modern research， the hot topics for aphasia treated with acupuncture-moxibustion are present and the achievements enriched. In future， these therapeutic methods should be further investigated to explore a model of translational medicine for aphasia in line with the characteristics of acupuncture-moxibustion.关键词：Acupuncture-moxibustion;Knowledge graph;Translational research;Aphasia;CiteSpace;VOSviewer63|6|0更新时间：2024-05-08
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