最新刊期
卷 48 ， 期 8 ， 2023
- 摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on white adipose tissue （WAT） browning by regulating central glucagon-like peptide-1 （GLP-1）， so as to explore the possible central mechanisms of EA in improving obesity.MethodsThirty male Wistar rats were randomly divided into normal group， model group， EA group， HM3D group， and EA+HM4D group， with 6 rats in each group. The obesity rat model was obtained by feeding with high-fat diet for 8 weeks. Adeno-associated virus combined with DREADDs was injected into bilateral nucleus of solitary tract （NTS）， with rAAV-GLP-1+rAAV-4D applied to the EA+HM4D group， rAAV-GLP-1+rAAV-3D applied to the HM3D group， and rAAV-GLP-1+rAAV-GFP applied to other 3 groups. After modeling， rats in the EA and EA+HM4D groups received EA treatment at bilateral “Zusanli”（ST36）， “Fenglong”（ST40）， “Guanyuan”（CV4） and “Zhongwan”（CV12）， with successive waves （2 Hz， 1 mA） for 10 minutes， 3 times a week， for a total of 8 weeks. Body mass of rats in each group were measured before and 2， 4， 6， and 8 weeks after intervention. Abdominal and perirenal WAT mass was weighed， serum triglyceride （TG） and total cholesterol （TC） contents were detected by using automatic analyzer， and nonestesterified fatty acid （NEFA） content was detected by using colorimetric assay kit. The morphology of abdominal WAT lipid droplets was observed by HE staining. The mRNA expressions of GLP-1 in NTS， AMPK in ventromedial nucleus of hypothalamus（VMH）， UCP1 and PGC-1α in subcutaneous fat were detected by real-time PCR. The protein expression levels of GLP-1， AMPK， phosphorylated-AMPK， UCP1 and PGC-1α were detected by Western blot. The activation level of GLP-1 neurons in NTS was observed by immunofluorescence.ResultsCompared with the normal group， abdominal WAT lipid droplets were enlarged， body weight， serum TG， TC， NEFA contents， abdominal and perirenal WAT mass， mRNA and protein expression levels of AMPK were significantly increased（P<0.01， P<0.05）， while GLP-1 neurons activation level， mRNA and protein expression levels of GLP-1， UCP1 and PGC-1α， and AMPK protein phosphorylation were decreased （P<0.01） in the model group. After EA intervention， body weight at 6 and 8 weeks after intervention and other indexes mentioned above were all significantly reversed （P<0.01， P<0.05） in the EA group in comparison with those of the model group. Compared with the EA group， the HM3D group had reduced abdominal WAT lipid droplets size， decreased serum TG， TC， and NEFA contents， and protein expression level of AMPK（P<0.01， P<0.05）， with increased mRNA and protein expression levels of GLP-1， UCP1 and PGC-1α， and phosphorylation level of AMPK protein（P<0.01， P<0.05）， while the EA+HM4D group had enlarged abdominal WAT lipid droplets， increased body weight 6 and 8 weeks after intervention， abdominal and renal WAT mass， and NEFA content （P<0.01， P<0.05）， with decreased serum TG content， activation level of GLP-1 neurons in the NTS， mRNA and protein expression levels of GLP-1， UCP1 and PGC-1α （P<0.01， P<0.05）， as well as down-regulated phosphorylation of AMPK protein and mRNA （P<0.01，P<0.05）.ConclusionEA can effectively promote the browning of WAT， which may be related to the activation of GLP-1 neurons in the NTS， as well as the promotion of the phosphorylation of AMPK in the VMH and up-regulation of UCP1.关键词：Electroacupuncture;Obesity;Lipid metabolism;Glucagon-like peptide-1;Browning of white adipose tissue;AMP-activated protein kinase101|18|0更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effects of moxibustion at “Tianshu”（ST25） and “Shangjuxu”（ST37） on the colonic metabolites and inflammatory factors in rats with Crohn’s disease（CD）， so as to explore the mechanisms of moxibustion in protecting colon of CD rats based on metabolomics.MethodsTwelve rats were first randomly selected from 36 male SD rats as a normal group（NG）. The CD model was induced by 2，4，6 trinitrobenzene sulfonic acid（TNBS） enema on the rest 24 rats. After successful modeling， rats were randomly divided into model（TNBS） and moxibustion（TNBS+MOX） groups（n=10 rats/group）. Moxibustion was applied at bilateral ST25 and ST37 for 30 min， once daily for 7 consecutive days in the TNBS+MOX group， while rats in the NG and TNBS groups did not receive any interventions. Body weight of rats was recorded and disease activity index（DAI） was assessed during the experiment. After interventions， HE staining was performed to observe pathological damage of colon. Serum levels of inflammatory factors were measured by ELISA. NMR hydrogen spectroscopy was used to detect colonic metabolites of each group， and orthogonal partial least squares discriminant analysis（OPLS-DA） was used to screen differential colonic metabolites between groups， followed by pathway analysis using MetaboAnalyst 5.0 platform.ResultsAfter modeling， compared with the NG group， the body weight of the rats in the TNBS group was significantly decreased（P<0.05）， the DAI score was increased （P<0.05）， the colon had obvious inflammatory damage and the pathological injury index was increased（P<0.05）， and levels of serum tumor necrosis factor-α（TNF-α）， interleukin（IL）-1β and interferon-γ（IFN-γ） were significantly increased（P<0.05）. After moxibustion intervention， compared with the TNBS group， the body weight was significantly increased（P<0.05）， while the levels of serum TNF-α， IL-1β， IFN-γ， and DAI score of the rats in the TNBS+MOX group were significantly decreased（P<0.05）， with alleviated colonic inflammatory injury detected by HE staining. Compared with the NG group， the relative expressions of colonic hypoxanthine， betaine， creatine， inositol， taurine， uracil， and methanol of the TNBS group were decreased（P<0.05）， while the relative expressions of histidine， leucine， proline， lysine， isoleucine， phenylalanine， tyrosine， propionic acid， and valine were increased（P<0.05） in the TNBS group， among which， relative expressions of hypoxanthine， leucine， lysine， isoleucine， betaine， tyrosine， and taurine were reversed in the TNBS+MOX group relevant to the TNBS group， mainly involving phenylalanine， tyrosine and tryptophan biosynthesis， and taurine and subtaurine metabolism pathway.ConclusionThe mechanism of moxibustion at ST25 and ST37 for CD may be related to improving colon metabolic disorder state by regulating multiple metabolic metabolites and metabolic pathways， and reducing the level of inflammatory factors， so as to maintain intestinal immune homeostasis.关键词：Crohn’s disease;Moxibustion;Tianshu（ST25）;Shangjuxu（ST37）;Metabolomics43|6|0更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effect of moxibustion at Governor Vessel acupoints on inositol requiring enzyme 1 （IRE1） / X-box binding protein 1 （XBP1） pathway in hippocampal CA1 region of rats with vascular dementia （VD）， so as to explore its mechanisms in the treatment of VD.MethodsMale SD rats were randomly divided into normal， sham operation， model， moxibustion （Moxi） and medication groups （n=12）. The VD model was established by permanent ligation of bilateral common carotid arteries. For rats of the Moxi group， mild moxibustion was given to “Baihui” （GV20）， “Dazhui” （GV14） and “Fengfu” （GV16） for 20 min each point， once a day for consecutive 6 days per week， for a total of 4 weeks. For rats of the medication group， intragastric perfusion of nimodipine was given 3 times each day with total dose of 2 mg·kg-1·d-1 for 4 weeks. Morris water maze test was used to detect the learning and memory ability of rats before and after modeling as well as after intervention. The apoptosis rate of nerve cells in hippocampal CA1 region was detected by TUNEL staining. The proteins and mRNA expression levels of IRE1， XBP1， B-cell lymphoma/leukemia-2 （Bcl-2）， Bcl-2 associated X protein （Bax） in hippocampal CA1 region were detected by Western blot and real-time quantitative PCR， respectively.ResultsCompared with the sham operation group， the average escape latency was significantly prolonged （P<0.01）， the number of times crossing the original platform was significantly reduced （P<0.01）， the apoptosis rate of nerve cells in hippocampal CA1 region was significantly increased （P<0.01）， the proteins and mRNA expression levels of IRE1， XBP1 and Bax were significantly increased （P<0.01）， and the expression levels of Bcl-2 protein and mRNA were significantly decreased （P<0.01） in rats of the model group. After treatment， compared with the model group， the average escape latency was significantly shortened （P<0.01）， the number of times crossing the original platform was increased （P<0.05）， the apoptosis rate of nerve cells in hippocampal CA1 region was significantly decreased （P<0.01）， the protein and mRNA expression levels of IRE1， XBP1 and Bax were decreased （P<0.05， P<0.01），and the expression levels of Bcl-2 protein and mRNA were increased （P<0.05， P<0.01） in rats of the Moxi group and medication group. There was no significant difference in the above indexes between the Moxi group and the medication group.ConclusionMoxibustion at the acupoints of Governor Vessel can improve the cognitive function of VD rats， and its mechanism may be related to regulating IRE1/XBP1 pathway， inhibiting the release of pro-apoptotic protein Bax， increasing the expression of anti-apoptotic protein Bcl-2， and thus inhibiting the apoptosis of hippocampal nerve cells.关键词：Vascular dementia;Moxibustion;Governor Vessel;Inositol requiring enzyme 1 / X-box binding protein 1 pathway;Nerve cell apoptosis32|7|2更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effect of electroacupuncture（EA）preconditioning on ferroptosis in rats with cerebral ischemia-reperfusion injury （CIRI）， so as to explore the neuroprotective mechanism of EA preconditioning.MethodsMale SD rats were randomly divided into sham operation， model， EA， inhibitor and inducer groups with 20 rats in each group. The CIRI model was established by modified Zea Longa occlusion of the middle cerebral artery. Before modeling， EA treatment （2 Hz/15 Hz， 1–2 mA） was applied to “Baihui”（GV20）， “Fengfu”（GV16） and “Dazhui”（GV14） for rats of the EA group， 20 min a day for 7 consecutive days. Rats of the inhibitor group were intraperitoneally injected with ferristatin-1（25 mg/kg）at a slow and uniform rate. Rats of the inducer group were intraperitoneally injected with Erastin（100 mg/kg） after 7 days of EA preconditioning， once every 2 h for a total of 4 times. The CIRI models were prepared 2 d later after the above interventions finished by thread-occlusion. The degree of neurological impairment was evaluated by modified Zea Longa score. The percentage of infarct size was calculated by TTC staining. The ultrastructure of neurons in hippocampus was observed by transmission electron microscope. The contents of ferrous ion （Fe2+）， malondialdehyde （MDA） and glutathione （GSH） in cerebral tissue and reactive oxygen species （ROS） in serum were determined by biochemical method. The changes of mitochondrial membrane potential in rats brain tissues were detected by flow cytometry. The mRNA and protein expression levels of glutathione peroxidase 4 （GPX4）， acyl-CoA synthetase long-chain family member 4 （ACSL4）， transferrin receptor （TFRC）， 15-lipoxygenase （15-LOX） and cyclooxygenase-2 （COX-2） in the ischemic hippocampal region of CIRI rats were detected by real-time quantitative PCR and Western blot， respectively.ResultsCompared with the sham operation group， the neurological impairment score， the percentage of cerebral infarction area， the contents of MDA and Fe2+ in cerebral tissue as well as ROS in serum， the protein and mRNA expression levels of ACSL4， TFRC， 15-LOX， COX-2 in hippocampal tissue were increased （P<0.01）， while the content of GSH in cerebral tissue， the protein and mRNA expression levels of GPX4 in hippocampal tissue were decreased （P<0.01）， and mitochondria in brain tissue were significantly damaged （P<0.01） in the model group. Compared with the model group， the above indexes were all reversed （P<0.05， P<0.01） in the EA group and inhibitor group. Compared with the EA group， the neurological impairment score， the percentage of cerebral infarction area， the contents of MDA and Fe2+ in cerebral tissue as well as ROS in serum， the protein and mRNA expression le-vels of ACSL4， TFRC， 15-LOX， COX-2 in hippocampal tissue were increased （P<0.05， P<0.01）， while the content of GSH in cerebral tissue， the protein and mRNA expression levels of GPX4 in hippocampal tissue were decreased （P<0.01， P<0.05）， and mitochondria in brain tissue were significantly damaged （P<0.05） in the inducer group.ConclusionEA preconditioning has neuroprotective effect on CIRI rats， which may be related to inhibiting ACSL4/TFRC/15-LOX/COX-2 expression and increasing GSH/GPX4 expression.关键词：Electroacupuncture pretreatment;Cerebral ischemia-reperfusion injury;Neurons;Ferroptosis75|9|2更新时间：2023-11-03
- 摘要：ObjectiveTo investigate the mechanism of electroacupuncture （EA） in promoting the browning of white adipose tissue in middle-aged and aged obese rats induced by high fat by regulating AMP-activated protein kinase （AMPK） /silence-information regulatory factor 1 （Sirt1） pathway and neuregulin 4 （Nrg4）.MethodsTwenty-four male SD rats were randomized into blank control， model and EA groups （n=8 per group）. The obesity model was established by feeding the rats with high-fat diet for 6 weeks. For the EA group， EA （2 Hz/15 Hz， 1.5 mA） was applied to “Guanyuan” （CV4） and bilateral “Shenshu” （BL23）， “Fenglong” （ST40） and “Tianshu” （ST25） for 20 min， once a day， 5 days a week for 6 weeks. Rats of the blank control and model groups were also restrained for 20 min. The body mass and food intake were measured every week， and the Lee’s index， epididymal fat， perirenal fat and brown adipose tissue were weighed. The contents of serum total cholesterol （TC）， triglyceride （TG）， high density lipoprotein cholesterol （HDL-C）， low density lipoprotein cholesterol （LDL-C） and norepinephrine （NE） were determined by ELISA. H.E. staining was used to observe the morphological changes of white and brown adipose tissue. The mRNA expression levels of mitochondrial uncoupling protein 1 （UCP1）， peroxisome proliferator activated receptor γ co-activator 1α （PGC-1α）， PR-domain protein 16 （PRDM16）， peroxisome proliferator activated receptor γ （PPARγ） and Nrg4 in the adipose tissue were detected by quantitative real time PCR， and the protein expression levels of Nrg4， AMPKα， Sirt1 and interleukin-6 （IL-6） in the white and brown adipose tissue were detected by Western blot.ResultsCompared with the blank control group， the body mass， food intake， the Lee’s index， epididymal fat and perirenal fat mass， and serum TG， TC and LDL-C contents and the expression level of IL-6 protein were significantly increased （P<0.01，P<0.05， P<0.001）， and the brown adipose mass， serum HDL-C and NE contents， the expression levels of UCP1， PGC-1α， PRDM16， PPARγ and Nrg4 mRNAs， and the protein expression levels of AMPKα， Sirt1 and Nrg4 proteins in both white and brown adipose tissues were significantly decreased in the model group （P<0.01， P<0.05）. After EA intervention， the increased levels of body mass， food intake， Lee’s index， epididymal fat and perirenal fat mass， serum TG， TC and LDL-C contents， and the expression of IL-6 protein， and the decreased levels of brown adipose mass， serum HDL-C and NE contents， expression levels of UCP1， PGC-1α， PRDM16， PPARγ and Nrg4 mRNAs， and those of AMPKα， Sirt1 and Nrg4 proteins in both white and brown adipose tissues were apparently reversed（P<0.05， P<0.01， P<0.001）. H.E. staining showed an increase of the volume and content of intracellular vacuoles of both white and brown adipose tissues， disordered arrangement of cells with vague boundary in the model group， which was relatively milder including a decrease of volume and content of vacuoles of both white and brown adipose， neat arrangement of cells with clear boundary.ConclusionEA intervention can improve lipid metabolism and promote white adipose tissue browning in middle-aged and aged obese rats， which is possibly associated with its functions in activating AMPK/Sirt1 signaling pathway and up-regulating the level of Nrg4.关键词：Obesity;Electroacupuncture;Lipid metabolism;White adipose tissue browning;AMPK/Sirt1 signaling pathway;Nrg454|7|0更新时间：2023-11-03
- 摘要：ObjectiveTo investigate whether herbal cake-separated moxibustion can activate nuclear factor erythroid 2-related factor 2 （Nrf2） / antioxidant responsive element （ARE） /hemeoxygenase-1 （HO-1） signaling pathway to repair aspirin induced gastric mucosal injury （GMI） in rats.MethodsSD rats （half male and half female） were randomly divided into blank control， model， moxibustion， inhibitor of HO-1 （inhibitor）， model+inhibitor， moxibustion + inhibitor groups， with 20 rats in each group. The GMI model was established by gavage of aspirin 150 mg/kg（1 mL/100 g）. Herbal cake-separated moxibustion was alternatively applied to bilateral “Zusanli” （ST36） and “Zhongwan” （CV12） and bilateral “Pishu” （BL20） and “Weishu” （BL21） for 30 min， once daily for 8 days. The rats in the three inhibitor groups received intraperitoneal injection of HO-1 inhibitor zinc protoporphyrin （5 mg/kg）. The rats’ behavior score， emotional response score， skin hair score， diet score and stool state score were given. The GMI index was calculated according to Guth’s methods. Histopathological changes of gastric mucosa were observed by H.E. staining. The activity of superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in the gastric mucosal tissue and serum were detected by ELISA. The levels of Nrf2/ARE/HO-1 signaling pathway-related factors Keap1， Nrf2， HO-1， CAT， GST， and NQO1 in the gastric mucosal tissue were detected by quantitative real-time PCR and Western blot， separately.ResultsCompared with the blank control group， the behavior score， emotional response score， skin hair score， diet score and stool state score， GMI index， MDA contents of gastric mucosal tissue and serum， expression le-vels of Keap1 mRNA and protein were significantly increased （P<0.01）， while the activity of gastric mucosal and serum SOD， the expression levels of Nrf2， HO-1， CAT， GST and NQO1 mRNAs and proteins were considerably decreased （P<0.01） in the model group. Compared with the model group， moxibustion obviously reversed the increase of emotional response score， skin hair score， stool state score， GMI index， MDA levels of gastric mucosal tissue and serum， and expression levels of Keap1 mRNA and protein （P<0.01，P<0.05）， and the decrease of activity of SOD of gastric mucosal and serum， and the expression levels of Nrf2， HO-1， CAT， GST and NQO1 mRNAs and proteins （P<0.01， P<0.05）. After administration of antagonist of HO-1， the effects of moxibustion were eliminated or weakened pronouncedly in reducing skin hair score， GMI index， contents of gastric mucosal and serum MDA， and expression of Keap1 mRNA and protein， and in up-regulating gastric mucosal and serum SOD， and expression of HO-1， CAT， GST and NQO1 mRNAs and proteins （P<0.01， P<0.05）.ConclusionHerbal-cake separated moxibustion can improve the GMI in rats， which may be associated with its effects in reducing oxidative stress and activating Nrf2/ARE/HO-1 signaling pathway.关键词：Gastric mucosa injury;Herbal cake-separated moxibustion;Nuclear factor erythroid 2-related factor 2/antioxidant responsive element/hemeoxygenase-1 signaling30|6|0更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effect of electroacupuncture（EA） on the expression of cytosolic phospholipase A2 （cPLA2） and apoptosis of nerve cells in rats with spinal cord injury （SCI）， so as to explore its mechanisms underlying improvement of SCI.MethodsSeventy-two female SD rats were randomly divided into model， EA， antagonist and EA+antagonist groups， with 18 rats in each group and other 18 rats were used as the sham operation （sham） group. The SCI model was established by referring to modified Allen’s method with a weight impactor. The hindlimb motor function was assessed by using Basso-Beattie-Bresnahan （BBB） score. Rats of the EA group were subjected to EA stimulation at “Dazhui”（GV14），“Yaoyangguan”（GV3），bilateral “Ciliao”（BL32） and “Zusanli”（ST36） for 20 min， once a day for 14 days. Rats of the antagonist group received intravenous injection followed by intraperitoneal injection of arachidonyl trifluoromethyl ketone （AACOCF3， antagonist of cPLA2）， once every other day. Rats of the EA+antagonist group received EA treatment combined with antagonist injection. After the treatment，the rats were sacrificed and the spinal cord tissue was collected for detecting the protein expression of cPLA2， p-cPLA2， Bcl-2， Bax and Caspase-3 by Western blot， and the mRNA expression of cPLA2， Bcl-2， Bax and Caspase-3 using qRT-PCR. The morphological changes of the spinal cord were detected by Nissl staining.ResultsIn comparison with the sham group， the BBB score， expression of Bcl-2 protein and mRNA were significantly down-regulated （P<0.01）， whereas the expression levels of Bax， Caspase-3 and p-cPLA2 proteins and mRNAs were considerably up-regulated in the model group （P<0.01）. Compared with the model group， the BBB score， expression levels of Bcl-2 protein and mRNA were significantly up-regulated （P<0.01，P<0.05）， while the expression levels of Bax， Caspase-3 and p-cPLA2 proteins in the EA， antagonist and EA+antagonist groups， Bax and cPLA2 mRNAs in both antagonist and EA+antagonist groups， and Caspase-3 mRNA in the EA+antagonist group were obviously down-regulated （P<0.01，P<0.05）. The effect of EA+antagonist was significantly superior to EA in increasing BBB score and in lowering expression of Bax and cPLA2 mRNAs （P<0.01， P<0.05）. Nissl staining showed reduced number of nerve cells and Nissl bodies， and striped dark blue cells in the model group， which was milder in the EA and antagonist groups， particularly in the EA+antagonist group.ConclusionEA may improve the limb motor function of SCI rats， which may be related to its functions in down-regulating the expression of p-cPLA2， Bax and Caspase-3 and up-regulating Bcl-2 to reduce the apoptosis of nerve cells in the regional spinal cord.关键词：Spinal cord injury;Electroacupuncture;Cytosolic phospholipase A2;Apoptosis26|4|3更新时间：2023-11-03
- 摘要：ObjectiveTo explore the mechanism of electroacupuncture（EA） in improving learning-memory ability in Alzheimer’s disease （AD） mice from the perspective of endosomal-lysosomal system.MethodsMale APP/PS1 transgenic mice were randomly divided into model group and EA group （n=10 in each group） and 10 male C57BL/6 wild mice were taken as the normal group. EA （1 Hz/50 Hz，1 mA） was applied at bilateral “Yongquan”（KI1） and acupuncture was applied at “Baihui” （GV20） for 15 min. The mice of the model and normal groups were subjected to restriction with the same method as those of the EA group for 15 min. The treatment was conducted once every other day for 6 weeks. The spatial learning-memory ability （shown by escape latency of place navigation test and the time of crossing the target platform and total swimming distance in the target quadrant in 1 min of spatial probe test ） was detected by Morris water maze test. The immunoactivity of senile plaques （SP） in the hippocampus tissue was detected by immunohistochemistry. The ultrastructural characters of hippocampal neurons were observed by transmission electron microscope， and the expression levels of Ras-related protein 5 （Rab5）， Ras-related protein 7 （Rab7） and cathepsin D （CTSD） in the hippocampus were detected by Western blot， separately.ResultsCompared with the normal group， the escape latency， SP immunoactivity， and protein expression levels of Rab5， Rab7 and CTSD were significantly increased （P<0.05， P<0.01）， while the number of crossing the original platform and the total swimming distance in the platform quadrant were considerably reduced （P<0.05） in the model group. In contrast to the model group， the EA group had a marked decrease in the escape latency， SP immunoactivity， and protein expression levels of Rab5， Rab7 and CTSD （P<0.05， P<0.01）， and a striking increase in the number of crossing the original platform and the swimming distance in the platform quadrant （P<0.05）. Results of transmission electron microscope showed an accumulation of endosome， lysosome， and endolysosomes in the hippocampal neurons in the model group， which was evidently milder in the EA group.ConclusionEA of GV20 and KI1 can improve the learning-memory ability of AD mice， which may be related to its function in reducing hippocampal Aβ deposition and down-regulating endosomal-lysosomal system activity.关键词：Alzheimer’s disease;Electroacupuncture;Endosomal-lysosomal system;Learning-memory ability;Senile plaques;Hippocampus37|4|1更新时间：2023-11-03
- 摘要：ObjectiveTo investigate the effects of acupuncture on JNK pathway and autophagy level in rats with intracerebral hemorrhage （ICH） and explore the partial mechanism of acupuncture against ICH.MethodsSD rats were randomly divided into blank group， model group and acupuncture group. Each group was divided into Day 1， Day 3 and Day 7 subgroups respectively， with 5 rats in each group. The autologous blood injection was adopted to duplicate rat model of ICH. In the acupuncture group， the needle was inserted from “Baihui” （GV20） towards “Qubin” （GB7） on the affected side， stimulating for 30 min each time， once daily； the same acupuncture technique was opera-ted in each subgroup for 1， 3 and 7 days， separately. Using Bederson scale， the neurological deficit was evaluated in each group. Western blot was adopted to detect the protein expression levels of Beclin1， LC3Ⅰ/Ⅱ， phosphorylated c-Jun amino-terminal kinase （p-JNK） and the phosphorylated （p）-c-Jun around hematoma lesion of the brain tissue of rats in each group.ResultsAfter treatment， the neurological deficit score of rats in the model group was higher than that of the blank group at each time point （P<0.05）， and the score of the acupuncture group started declining since the 3rd day of treatment when compared with the model group （P<0.05）. At each time point， compared with the blank group， the protein expression levels of LC3Ⅰ/Ⅱ， Beclin1， p-c-Jun and p-JNK was increased （P<0.01）. Compared with the model group， the protein expression level of LC3Ⅰ/Ⅱ was reduced （P<0.05）； the protein expression levels of Beclin1，p-c-Jun and p-JNK was increased （P<0.05，P<0.01） on day 3 and 7 in the acupuncture group.ConclusionAcupuncture can activate the JNK pathway in the brain tissue of rats with ICH and increase the level of autophagy， thereby improving the neurological function of the rats with ICH.关键词：Intracerebral hemorrhage;Acupuncture;Autophagy;c-Jun amino-terminal kinase-pathway28|6|1更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effects of electroacupuncture （EA） on hormone secretion function of ovarian granulosa cells and theca cells， as well as the expression changes of kisspeptin and kiss1r in rats with polycystic ovarian syndrome （PCOS）， so as to explore the mechanism of EA for relieving ovarian dysfunction in PCOS rats.MethodsForty-eight SD female rats were randomly divided into control group， model group， EA group and flutamide group， with 12 rats in each group. PCOS rat model was replicated with the gavage of letrozole （0.1 mg/mL， 10 mL·kg-1·d-1）. In the EA group， EA （2 Hz， 2 mA） was used to stimulate “Guanyuan” （CV4） for 20 min each time. In the flutamide group， flutamide solution （50 mg·kg-1·d-1） was administrated by gavage. The treatments were given once daily for 14 days in each group. After the modeling and treatment， the body and ovarian weights of the rats were measured， and the ovarian index was calculated. Using HE staining， the morphological changes of ovary were observed. ELISA was adopted to detect the contents of testosterone （T）， luteinizing hormone （LH） and estradiol （E2） in serum， the contents of E2 and T in the culture medium of ovarian granulosa cells and theca cells， as well as the content of kisspeptin in the ovarian tissue. The positive expression of kisspeptin in ovary was observed by immunohistochemical method， and the protein expression of its receptor kiss1r was detected by Western blot.ResultsCompared with the control group， the body and ovarian weights， ovarian index， the contents of T and LH in serum and that of T in the culture medium of theca cells， as well as the content and positive expression of kisspeptin in ovary were all increased （P<0.01， P<0.05）； and the content of E2 in the culture medium of granulosa cells was decreased （P<0.01） in the model group. When compared with the model group， in the EA and flutamide groups， the body and ovarian weights， ovarian index， the contents of T and LH in serum and that of T in the culture medium of theca cells， as well as the content and expression of kisspeptin in ovary were all decreased （P<0.01， P<0.05）； and the content of E2 in the culture medium of granulosa cells was increased （P<0.05， P<0.01）.ConclusionEA regulates the serum sex hormone levels， the secretion function of the ovarian granulosa cells and theca cells， and the ovarian kisspeptin/kiss1r protein expression in PCOS rats， showing the similar effect as receptor blockade intervention. It is suggested that the improvement of EA in ovarian dysfunction of PCOS rats may be related to the kisspeptin/kiss1r system.关键词：Polycystic ovarian syndrome;Electroacupuncture;Sex hormones;Kisspeptin35|5|0更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effects of transcutaneous auricular vagus nerve stimulation （taVNS） on plasma melatonin （MLT） content and insulin receptor expression in the liver， the skeletal muscles， and the pancreas of Zucker diabetic fatty （ZDF） rats， so as to explore the hypoglycemic mechanism of taVNS.MethodsThirty male ZDF rats were randomly divided into model group， taVNS group and sham-taVNS group， with 10 rats in each group； besides， 10 male Zucker lean rats of the same strain were collected for the blank control group. ZDF rats were fed with high-fat diet to induce type 2 diabetes mellitus （T2DM） rat model. In the taVNS group， HANS-100A electroacupuncture instrument was used to stimulate the cavum conchae of both sides. The stimulation sites of rats in the sham-taVNS were the same as the taVNS group， but without electricity delivered. The above interventions were performed 30 min each time， once daily， lasting for 6 weeks. Fasting blood glucose （FBG） was measured weekly in each group， the plasma metatonin （MLT） content was detected by ELISA， and the insulin receptor expression level in the liver， the skeletal muscle and the pancreas was determined by Western blot.ResultsCompared with the blank control group， the level of FBG of rats were increased （P<0.01）， the plasma MLT content was decreased （P<0.01） and the insulin receptor expression level in the pancreatic tissue was decreased （P<0.01） in the model group. In the taVNS gruop， FBG was decreased （P<0.05， P<0.01）， the plasma MLT content was increased （P<0.01）， and the insulin receptor expression level in the liver， the skeletal muscle and the pancreas was increased （P<0.05， P<0.01， P<0.001） when compared with the model group. Compared with the taVNS group， FBG was increased （P<0.05， P<0.01）， the plasma MLT content was decreased （P<0.01）， and the expression level of insulin receptors in the skeletal muscle and the pancreas was decreased （P<0.01， P<0.001） in the sham-taVNS group.ConclusionThe taVNS can improve the insulin resistance and ultimately obtain the antihyperglycemic effect through regulating MLT concentration.关键词：Transcutaneous auricular vagus nerve stimulation;Type 2 diabetes mellitus;Melatonin;Vagus nerve43|7|0更新时间：2023-11-03
- 摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Zhongwan” （CV12）， “Tianshu” （ST25） and “Shangjuxu” （ST37） （an acupoint prescription “Changbingfang” for treatment of intestinal disorders） on autophagy and expression of AMPK/mTOR signaling pathway in rats with ulcerative colitis （UC）， so as to explore its mechanism underlying improvement of UC.MethodsThirty-two male SD rats were randomly divided into control， model， medication and EA groups， with 8 rats in each group. The UC model was established by free drinking of 5% dextran sulfate sodium salt solution for 7 days. EA stimulation （10 Hz/50 Hz） was delivered to CV12， ST25 and ST37 for 20 min， once a day for 3 consecutive days. Rats of the medication group received gavage of mesalazine suspension （200 mg/kg） once a day， 3 times in total. The rats’ general conditions were recorded for calculating the disease activity index （DAI） score （0—4 points）. Histomorphological changes of colon were observed via HE staining. The levels of serum interleukin 6 （IL-6）， tumor necrosis factor-α （TNF-α） and IL-10 were measured by ELISA. The mRNA expressions of LC3B and p62 were tested by fluorescence quantitative PCR. Western blot was used to detect the expression levels of LC3B， p62 and AMPK/mTOR pathway related proteins in colon tissues.ResultsCompared with the control group， the DAI score， contents of serum IL-6 and TNF-α， the expression levels of p62 protein and mRNA， ratio of p-mTOR/mTOR were significantly increased （P<0.01）； while the content of serum IL-10， the expression levels of LC3B mRNA， ratio of LC3BⅡ/LC3BⅠ and p-AMPK/AMPK were decreased （P<0.01， P<0.05） in the model group. Relevant to the model group， modeling-induced increases of DAI score， serum IL-6， TNF-α and IL-10 contents， expressions of p62 protein and mRNA， LC3B mRNA， ratio of p-mTOR/mTOR， LC3BⅡ/LC3BⅠ and p-AMPK/AMPK were reversed in both medication and EA groups （P<0.01， P<0.05）. The effect of EA was apparently superior to that of mesalazine in up-regulating ratio of LC3BⅡ/LC3BⅠ and p-AMPK/AMPK， p62 mRNA expression （P<0.01， P<0.05）， and in down-regulating ratio of p-mTOR/mTOR （P<0.05）. H.E. staining showed severe damage of the colonic mucosal barrier with infiltration of a large number of inflammatory cells in the model group， which was milder in medication and EA groups.ConclusionEA of acupoint recipe “Changbingfang” can improve the symptoms in UC rats， which may be related to its functions in promoting colonic autophagy， increasing AMPK phosphorylation level， and decreasing mTOR phosphorylation level.关键词：Ulcerative colitis;Autophagy;Electroacupunture;Acupoint prescription “Changbingfang”;Adenosin emonophosphate-activated protein kinase/mammalian target of rapamycin signaling pathway62|12|4更新时间：2023-11-03
- 摘要：Modern acupuncture anesthesia is the application of acupuncture-related therapies to optimize the perioperative management which is based on the combined acupuncture-medicine anesthesia technology， and building a perioperative acupuncture anesthesia accelerated rehabilitation system. Based on the thoracic surgery， this paper analyzes and summarizes the application effects of modern acupuncture anesthesia， focusing on preoperative anxiety relief and advanced analgesia； reduce the dosage of anesthetics， stable respiration and hemodynamics， anti-stress and organ protection during surgery； postoperative analgesia，prevention of nausea，vomiting and cognitive impairment，improvement of gastrointestinal function， prevention of cognitive impairment， and enhancement of immunity. It is anticipated that this review may provide a basis for the further promotion and application of modern acupuncture anesthesia in clinical practice.关键词：Modern acupuncture anesthesia;Thoracic surgery;Perioperative period;Clinical practice89|17|1更新时间：2023-11-03
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