最新刊期
卷 48 ， 期 9 ， 2023
- 摘要：ObjectiveTo investigate the relationship between the sensitization state of acupoints on the surface of the myocardial ischemia （MI） model mice and the changes in the electrophysiological properties of the dorsal root ganglion （DRG） neurons in the corresponding spinal cord segment， and its underlying mechanism.MethodsSixty-eight male C57BL/6J mice were randomly divided into control and model groups （34 mice in each group）. The model group received an intraperitoneal injection of 160 mg/kg isoproterenol （ISO） to establish the MI model， and the control group received an injection of the same dose of normal saline as the model group. After modeling for about 6 days， MI proportion was measured by HE staining to verify the pathological changes in the heart tissue. Evans blue （EB） dye was injected into the tail vein of mice to reflect the size， location， distribution， and number of exudates on the body surface. Then， whole-cell membrane currents， intrinsic excitability and membrane properties of different types of DRG neurons were evaluated by electrophysiological experiment in vitro.ResultsCompared with the control group， the heart size was larger， with pathological outcomes showing enlarged myocardial hypertrophy， destroyed structure of cardiomyocytes， with mononuclear cell infiltration among the cardiomyocytes in the model group. Compared with the control group， the number of EB exudation points was significantly increased （P<0.01）， which were mainly concentrated in the epidermis near the T1—T5 segment of the spinal cord， “Feishu” （BL13）， “Jueyinshu” （BL14） and “Xinshu” （BL15） in the model group. Compared with the control group， the rheobase and action potential amplitude （APA） of DRG medium-sized neurons were obviously decreased （P<0.01，P<0.05）， while the whole-cell membrane currents， the spike numbers， the average instantaneous frequency， and the average discharge frequency were markedly increased （P<0.01）. There were no significant alterations in the membrane properties and intrinsic excitability induced by depolarized currents of small-sized neurons between groups. Compared with the control group， the whole-cell membrane currents， spike numbers， and the average instantaneous frequency were significantly increased in the model group（P<0.05， P<0.01） while rheobase was significantly decreased （P<0.05） in DRG medium-sized neurons labeled with biotin and CGRP.ConclusionAfter the mice were modeled by ISO， the DRG medium-size neurons in the T1—T5 segment of the spinal cord may mediate the sensitization of acupoints on the body surface through their different neuronal membrane properties and intrinsic excitabilities.关键词：Myocardial ischemia;Acupoint sensitization;Dorsal root ganglia;Intrinsic excitability of neurons112|13|1更新时间：2023-09-18
- 摘要：ObjectiveTo observe the effect of electroacupuncture on brain-derived neurotrophin factor （BDNF） / tyrosine kinase receptor B （TRKB） / cyclic adenosine monophosphate response element binding protein （CREB） pathway， synaptic plasticity marker protein and synaptic ultrastructure in the hippocampus of rats with learning and memory impairment induced by cerebral ischemia reperfusion （IR）， so as to explore its mechanisms underlying improvement of cognitive impairment after stroke.MethodsSD rats were randomly divided into blank， sham operation， model， and EA groups， with 12 rats in each group. The model of IR was established by occlusion of the middle cerebral artery. EA （2 Hz/10 Hz， 1—3 mA） was applied to “Shenting” （GV24） and “Baihui” （GV20） for 30 min， once daily for 14 days. The neurological function was evaluated according to the Zea Longa’s score criteria. Morris water maze test was used to detect the learning and memory function of the rats. Nissl staining was used to observe the pathological morphology of the hippocampus. Transmission electron microscopy was used to observe the ultrastructure of the syna-pse in the hippocampus， the synaptic gap width and postsynaptic dense substance （PSD） thickness were measured. Immunofluorescence staining was used to observe the positive expression levels of BDNF， PSD-95 and synaptophysin （SYN） in hippocampal CA1 region. The protein expression levels of BDNF， TRKB， CREB， PSD-95， and SYN in hippocampal tissue were detected by Western blot.ResultsCompared with the sham operation group， the neurological function score and escape latency （EL） were significantly increased （P<0.01）， the times of crossing the original platform were decreased （P<0.01）， the number of neurons in the CA1 area of the hippocampus was reduced， with incomplete morphology， widened synaptic gaps and significantly decreased PSD thickness （P<0.01）， the positive expressions of BDNF， PSD-95， SYN and the protein expression levels of BDNF， TRKB， CREB， PSD-95， SYN were significantly decreased （P<0.01） in the model group. Compared with the model group， the neurological function scores and EL on the 12th and 13th day were decreased （P<0.01， P<0.05）， the times of crossing the original platform were increased （P<0.01）， the morphology of hippocampal CA1 neurons improved， the synaptic gaps was decreased （P<0.01）， the PSD thickness was significantly increased （P<0.01）， the positive expressions of BDNF， PSD-95， SYN， and the protein expression levels of BDNF， TRKB， CREB， PSD-95， SYN were increased （P<0.05， P<0.01） in the EA group.ConclusionEA can alleviate cognitive impairment in IR rats， which may be related to its function in up-regulating the proteins of BDNF/TRKB/CREB pathway， promoting the expressions of synaptic plasticity marker proteins PSD-95 and SYN， thus improving the synaptic plasticity.关键词：Cerebral ischemia-reperfusion injury;Electroacupuncture;Synaptic plasticity;BDNF/TRKB/CREB;Learning and memory impairment115|22|6更新时间：2023-09-18
- 摘要：ObjectiveTo explore the molecular mechanism of electrical stimulation with scalp acupuncture （ESA） in alleviating neuroinflammatory injury in ischemic stroke rats based on interferon γ （IFN-γ）-mediated Janus kinase/signal transduction and transcriptional activator 1 （JAK/STAT1） signaling pathway.MethodsFifty-six SD rats aged 7 weeks were randomly divided into normal， model， ESA and inhibitor groups， with 14 rats in each group. The middle cerebral artery embolization rat model was established by means of thread embolization. Rats in the inhibitor group were intraperitoneally injected with the inhibitor PJ34 （5 mg/mL， 25 mg/kg） once a day for 7 days. Rats in the ESA group were treated at bilateral anterior parietotemporal slash （MS6） with ESA （2 Hz/100 Hz， 1 mA）， 30 min a day for 7 days. Before and after interventions， the neurological deficit score and neurobehavioral score were evaluated. The percentage of cerebral infarction volume was detected by TTC staining. The positive expressions of interleukin （IL）-6 and IL-10 in cerebral cortex were detected by immunohistochemistry. The protein expression levels of IFN-γ， JAK1， JAK2 and phosphorylated （p）-STAT1 in rats cerebral cortex were detected by Western blot.ResultsCompared with the normal group， the neurological deficit score， neurobehavioral score， the percentage of cerebral infarction volume， the expression levels of IL-6， IFN-γ， JAK1， JAK2 and p-STAT1 in cerebral cortex were increased （P<0.01）， while the expression level of IL-10 was decreased （P<0.01） in the model group. Compared with the model group， the neurological deficit score and neurobehavioral score after treatment were significantly decreased （P<0.01）， the percentage of cerebral infarction volume was decreased （P<0.01）， the expression levels of IL-6， IFN-γ， JAK1， JAK2 and p-STAT1 in cerebral cortex were decreased （P<0.01）， while the expression level of IL-10 was increased （P<0.01） in the ESA and inhibitor groups. ESA was superior to inhibitors in improving neurological deficit score and down-regulating p-STAT1 expression （P<0.05， P<0.01）， and was inferior to inhibitor in reducing the percentage of cerebral infarction volume as well as down-regulating IFN-γ and JAK1 （P<0.01， P<0.05）.ConclusionDown-regulating the expression of IFN-γ and inhibiting the activity of JAK/STAT1 signaling pathway may be one of the mechanisms by which ESA alleviates neuroinflammatory injury in ischemic stroke rats.关键词：Ischemic stroke;Electrical stimulation with scalp acupuncture;Neuroinflammatory injury;Janus kinase/signal transduction and activator of transcription 1 signaling pathway50|4|1更新时间：2023-09-18
- 摘要：ObjectiveTo observe the effect of electroacupuncture （EA） stimulation of Governor Vessel on chloridion （Cl-） homeostasis and the expression of γ-aminobutyric acid （GABA） and Na+-K+-Cl- cotransporter 1 （NKCC1） in the cerebral cortex of cerebral ischemia-reperfusion injury （CIRI） model rats， so as to explore its mechanism underl-ying alleviating limb spasm after stroke.MethodsForty-five male SD rats were randomly divided into normal， sham-operation， model， EA and baclofen groups， with 9 rats in each group. The CIRI model was established by occlusion of the middle cerebral artery and reperfusion. EA（100 Hz） was applied to “Dazhui” （GV14）， “Jizhong”（GV6） and “Houhui” for 30 min. Rats of the baclofen group received gavage of baclofen solution （0.4 mg/kg， 1 mL/100 g）， once daily for 7 consecutive days. Neurological deficit score was assessed according to Zea Longa’s method. The muscular tone of quadriceps femoris of the limb was evaluated by modified Ashworth scale and electrophysiological recor-ding methods， separately. TTC staining was used to detect cerebral infarction volume， and the brain water content of rats in each group was determined by wet and dry weight method. The contents of Cl- and GABA in the cerebral cortex were detected by colorimetric method， and the expression levels of NKCC1 mRNA and protein in the cerebral cortex were detected by quantitative real-time PCR and Western blot， separately.ResultsNo significant differences were found between the normal and sham-operation groups in all the indexes. Compared with the normal and sham-operation groups， the neurological deficit score， modified Ashworth muscle tone score， brain water content， cerebral infarct volu-me percent， Cl- content and expression levels of NKCC1 mRNA and protein were all evidently increased （P<0.01）， and muscle tone of electrophyiological electromyogram （EMG） signal and GABA content were strikingly decreased （P<0.01） in the model group. Compared with the model group， both EA and baclofen groups had an obvious increase in EMG signal displayed muscle tone， and GABA content （P<0.05， P<0.01）， and a marked decrease in the neurological deficit score， modified Ashworth score， brain water content， cerebral infarct percent， Cl- content and expression levels of NKCC1 mRNA and protein （P<0.05， P<0.01）.ConclusionEA stimulation of acupoints of the Governor Vessel can improve the degree of limb spasm and reduce the degree of cerebral edema and infarction in rats with stroke， which may be related to its functions in protecting Cl- homeostasis， up-regulating GABA concentration， and down-regulating the expression of NKCC1 protein and mRNA in the cerebral cortex.关键词：Electroacupuncture;Stroke;Limb spasm;Cerebral chloridion homeostasis;γ-aminobutyric acid;Na+-K+-Cl- cotransporter 151|4|1更新时间：2023-09-18
- 摘要：ObjectiveIt is to explore， based on stromal cell derived factor 1 （SDF-1）/CXC chemokine receptor 4 （CXCR4） signal axis， whether the electroacupuncture （EA） combined with bone marrow mesenchymal stem cells （BMSCs） transplantation can promote thin endometrium regeneration and improve endometrial receptivity， so as to further study its mechanisms underlying improvement of promoting BMSCs homing to repair thin endometrium.MethodsThirty matured female SD rats were randomly divided into normal control ， model ， BMSCs transplantation （BMSCs）， BMSCs+AMD3100 （a specific antagonist of CXCR4， BMSCs+AMD3100）， BMSCs+EA， and BMSCs+EA+AMD3100 groups， with 5 rats in each group. The thin endometrial model was established by intrauterine injection of 95% ethanol during the period of estrus. Rats of the model group received intravenous injection of PBS solution （tail vein） on day 1， 3 and 7 of modeling and intraperitoneal injection of normal saline once daily for 3 estrous cycles. Rats of the BMSCs group received intravenous injection of BMSCs suspension on day 1，3 and 7 of modeling， and those of the BMSCs+EA group received BMSCs transplantation and EA stimulation. EA （2 Hz/15 Hz， 1 mA） was applied to “Guanyuan” （CV4） and bilateral “Sanyinjiao”（SP9）， “Zigong” （EX-CA1） for 15 min， once daily for 3 estrous cycles. Rats of the BMSCs+AMD3100 group received intravenous injection of BMSCs suspension （1×106/mL） and intraperitoneal injection of AMD3100 （5 mg/kg）， and those of the BMSCs+EA+AMD3100 group received administration of BMSCs， AMD3100 and EA， with both groups being once daily for 3 estrous cycles. H.E. staining was used to observe histopathological changes of endometrium tissues， and immunohistochemistry was used to detect the expressions of cytokeratin （CK19） and vimentin in endometrium （for evaluating the damage and repair of endometrium）. The expression levels of homeobox A10 （HOXA10）， leukemia inhibitory factor （LIF）， SDF-1 and CXCR4 proteins were detected by Western blot， and those of SDF-1 and CXCR4 mRNAs in the endometrium detected by real-time PCR.ResultsIn comparison with the normal control group， the number of endometrial glands， the immunoactivity of CK19 and vimentin， the expression leve-ls of HOXA10， LIF and CXCR4 proteins and CXCR4 mRNA were significantly down-regulated （P<0.01）， and the expression levels of SDF-1 protein and mRNA significantly up-regulated （P<0.05） in the model group. Compared with the model group， the number of endometrial glands， the immunoactivity of CK19 and vimentin， and the expression levels of HOXA10， LIF， CXCR4 proteins and CXCR4 mRNA in the BMSCs group， and the number of endometrial glands， the immunoactivity of CK19 and vimentin， the expression levels of HOXA10， LIF， CXCR4 proteins and CXCR4 mRNA， and SDF-1 protein and mRNA in the BMSCs+EA group were significantly up-regulated （P<0.05， P<0.01）. Compared to the BMSCs group， the number of endometrial glands， and the expression levels of LIF， CXCR4 proteins and CXCR4 mRNA in the BMSCs+EA group were up-regulated （P<0.01， P<0.05）； the number of endometrial glands， the immunoactivity of CK19 and vimentin， the expression levels of HOXA10， LIF， CXCR4 proteins and CXCR4 mRNA in the BMSCs+AMD3100 group were down-regulated （P<0.01）. Compared to the BMSCs+EA group， the number of endometrial glands， the immunoactivity of CK19 and vimentin， the expression levels of HOXA10， LIF， CXCR4 proteins and CXCR4 mRNA in the BMSCs+EA+AMD3100 group were down-regulated （P<0.01）. Results of H.E. staining showed thin endometrium with absence of epithelial cells， and sparse glands and blood vessels， with smaller glandular cavity in the model group， which was relative milder in BMSCs and BMSCs+EA groups.ConclusionEA can promote the transfer of transplanted BMSCs to the damaged site through SDF-1/CXCR4 signaling related stem cell homing， thereby promoting thin endometrial regeneration， repairing endometrial injury， and improving endometrial tolerance in rats with thin endometrium.关键词：Electroacupuncture;Thin endometrium;Bone marrow mesenchymal stem cells;Chemotaxis;Stromal cell derived factor 1;CXC chemokine receptor 448|7|1更新时间：2023-09-18
- 摘要：ObjectiveTo explore the mechanism of moxibustion in the treatment of asthmatic inflammation from the point of short-chain fatty acids （SCFAs） in rats with asthma.MethodsA total of 48 SD rats （half male and half female） were randomly divided into 4 groups： normal， model， lung treatment and joint-treatment of lung and intestine （joint-treatment）， with 12 rats in each group. The asthma model was made by subcutaneous （bilateral back and inguinal regions） and intraperitoneal injection of mixture solution of ovalbumin and aluminium hydroxide gel （on day 1 and 8） and followed by inhalation of atomized 1% ovalbumin （20 min from day 15， once daily for one week）. Moxibustion was applied to bilateral "Feishu" （BL13） for rats of the lung treatment group or bilateral "Feishu" （BL13） and "Tianshu" （ST25） for rats of the joint treatment group. One hour after the intervention， the rats in the later three groups were separately given atomized 1% ovalbumin solution inhalation for 20 min. The treatment was conducted for 30 min， once daily for 14 consecutive days. At the end of the intervention， the percentage of inflammatory cells in blood was detected by biochemical method and histopathological changes of the lung were observed after H.E. staining. The inflammatory cells in the bronchoalveolar lavage fluid （BALF） were counted after Wright-Giemsa staining. The mRNA expressions of interleukin （IL）-4， IL-5， IL-13， IL-17， IL-33， leukotriene （LT）， thymic stromal lymphopoietin （TSLP） and prostaglandin D2 （PGD2） were detected by real-time PCR， and the contents of SCFAs in rats’ feces were detected by gas chromatography-mass spectrometry.ResultsRelevant to the normal group， the model group had an obvious increase in the percentages of neutrophils， lymphocytes and eosinophils in the blood， the percentages of neutrophils and eosinophils in the BALF， and in the expression levels of PGD2， TSLP， LT， IL-4， IL-5， IL-13， IL-17 and IL-33 mRNAs in the lung tissues （P<0.01， P<0.05）， and a marked decrease in the contents of acetic acid， propionic acid， isobutyric acid， butyric acid and valeric acid in feces （P<0.05， P<0.01）. After the treatment， the percentages of neutrophils and lymphocytes in the peripheral blood， eosinophils in the BALF， and the expression levels of PGD2， TSLP， LT， IL-4， IL-17， IL-33 mRNAs in the lung tissues in both the lung treatment and joint treatment groups， as well as neutrophils of BALF， and expression of IL-5 and IL-13 mRNAs in the joint treatment group were significantly down-regulated （P<0.01， P<0.05）， while the contents of acetic acid， propionic acid and valerate in the lung treatment group， and acetic acid， propionic acid， isobutyric acid， butyric acid and valeric acid in the joint treatment group were all strikingly increased （P<0.05， P<0.01）. The effect of the joint treatment was superior to that of lung treatment in down-regulating the expressions of LT and IL-5 mRNAs （P<0.05， P<0.01） and up-requlating the content of propionic acid （P<0.05）. Results of H.E. staining showed thickened alveolar wall， infiltration of a large number of inflammatory cells and interstitial fibrous tissue hyperplasia around the bronchus and scattered arrangement of cells of the lung tissue in the model group， which was relatively milder in both lung treatment and joint treatment groups， particularly the later.ConclusionJoint treatment of asthma from the lung and intestine can better regulate the contents of intestinal SCFAs and alleviate the inflammatory response of asthmatic model rats， thus， intestinal SCFAs may be involved in the process of moxibustion in improving inflammatory response.关键词：Asthma;Moxibustion;Lung-intestine treatment;Inflammation;Intestinal short-chain fatty acids;Bromchoalveolar lavage fluid32|2|3更新时间：2023-09-18
- 摘要：ObjectiveTo investigate the effect of electroacupuncture（EA） stimulation on proliferation and diffe-rentiation of endogenous neural stem cells as well as Jagged1/Notch1 pathway in AD model mice， so as to explore its mechanism underlying amelioration of AD.MethodsA total of 40 6-week-old male APP/PS1 transgenic AD mice were randomly divided into EA group （n=20） and AD model group （ n=20），and other 20 normal C57BL/6J mice of the same age were used as the normal control group. The mice in the EA group received EA （10 Hz， 2 mA） at "Baihui"（GV20）， "Fengfu"（GV16） and bilateral "Shenshu" （BL23） for 20 min， once daily， 6 days a week for 16 weeks. The mice’s learning-memory ability was detected by Morris water maze tests. The Aβ senile plaques in the hippocampal CA1 region were detected by Congo red staining， the immunofluorescence double label of BrdU， neuronal nuclear antigen （NeuN） and astrocyte specific protein GFAP in dentate gyrus of hippocampus was performed for detecting the proliferation and differentiation of the endogenous neural stem cells. The expression levels of Nestin （neuron specific protein） and GFAP were detected by Western blot， and those of Jagged1 and Notch1 mRNAs and proteins in the hippocampus were detected by real-time fluorescence quantifative PCR and Western blot.ResultsCompared with the normal control group， the escape latencies at 2nd， 3rd and 4th day， and Aβ senile plaques were significantly increased （P<0.05， P<0.01）， whereas the platform crossing times and time spent in the target quadrant， the expression levels of Jagged1 mRNA and Nestin protein were remarkably down-regulated （P<0.05） in the model group. Following EA intervention， the escape latencies at the 3rd and 4th day， Aβ senile plaques， immunofluorescence density of BrdU/GFAP， and GFAP protein expression were pronouncedly decreased （P<0.05， P<0.01）， while the platform crossing times， platform quadrant residence time， immunofluorescence density of BrdU/NeuN， expression levels of Jagged1 and Notch1 mRNAs and proteins and Nestin protein evidently increased （P<0.05， P<0.01）， suggesting an enhancement of proliferation and diffe-rentiation of endogenous neural stem cells into neurons and a suppression of the proliferation and differentiation towards astrocytes in the hippocampus.ConclusionEA at GV20， GV16 and BL23 can improve the learning-memory ability， promote the proliferation and differentiation of endogenous neural stem cells towards neurons and inhibit the proliferation and differentiation of endogenous neural stem cells towards astrocytes in the hippocampus， which may be achieved by regulating Jagged1/Notch1 pathway.关键词：Alzheimer's disease;Electroacupuncture;Learning-memory ability;Neural stem cells;Notch pathway50|3|1更新时间：2023-09-18
- 摘要：ObjectiveTo observe the effect of acupotomy on mitophagy mediated by PINK1/Parkin pathway in cartilage of rabbits with knee osteoarthritis （KOA）， so as to explore its mechanism in inhibiting cartilage damage.MethodsTwenty-one New Zealand rabbits were randomly divided into normal， model， and acupotomy groups， with 7 rabbits in each group. The KOA rabbit model was established by using the Videman method. Rabbits in the acupotomy group received regular acupotomy treatment around the knee joint nodules or tendons once a week for 3 consecutive weeks. HE staining and transmission electron microscopy were used to observe the morphological and ultrastructural changes in knee joint cartilage of rabbits. Flow cytometry was used to measure the mitochondrial membrane potential （Δψm） and reactive oxygen species （ROS） average fluorescence intensity in chondrocytes. Immunofluorescence was performed to detect the fluorescence intensity of LC3B， PINK1 and Parkin in cartilage tissue. Western blot was conducted to measure the protein expression levels of p62， LC3Ⅱ/Ⅰ， PINK1， and Parkin in cartilage tissue.ResultsCompared to the normal group， the model group showed fissures and tissue fibrosis on the surface of rabbit knee joint cartilages， loose distribution of chondrocytes， decreased autophagosomes， and abnormal mitochondrial morphology. The fluorescence intensity of LC3B， PINK1 and Parkin， the expression levels of LC3Ⅱ/Ⅰ， PINK1 and Parkin proteins in cartilage tissue were significantly decreased （P<0.01）， while the percentage of chondrocytes with low Δψm， the average fluorescence intensity of ROS， and the expression of p62 protein in cartilage tissue were significantly increased （P<0.01）. Compared to the model group， the acupotomy group showed no obvious defects on the surface of rabbit knee joint cartilage， relatively dense distribution of chondrocytes， increased autophagosomes， and relatively normal mitochondrial morphology. The fluorescence intensity of LC3B， PINK1 and Parkin， the expression of LC3Ⅱ/Ⅰ， PINK1 and Parkin proteins in cartilage tissue were significantly increased （P<0.01， P<0.05）， while the percentage of chondrocytes with low Δψm， the average fluorescence intensity of ROS， and the expression of p62 protein in cartilage tissue were significantly decreased （P<0.01）.ConclusionAcupotomy may promote mitophagy by regulating the PINK1/Parkin pathway， thereby improving cartilage damage in rabbits with KOA.关键词：Acupotomy;Knee osteoarthritis;Cartilage;PINK1/Parkin pathway;Mitophagy37|3|1更新时间：2023-09-18
- 摘要：ObjectiveTo observe the effect of electroacupuncture（EA） on activities of A2 type astrocytes（A2s）and A1 type astrocytes （A1s） ， expressions of neurofilament protein 200 （NF-200， a marker of axon regeneration）， nexin 1（NL1， a marker of synaptic regeneration）， and regeneration of Nissl bodies in rats with spinal cord injury （SCI）， so as to explore its mechanisms underlying improvement of SCI.MethodsA total of 75 male SD rats were rando-mized into sham operation， model， antibody neutralizing （AN）， EA and EA+AN groups， with 15 rats in each group. The SCI model was established by using an infinite field impactor to deliver an about 200 k dyne weight onto the exposed spinal cord after making a dorsal laminectomy at vertebral level T10. EA （2 Hz， 1 mA） was applied to“Dazhui”（GV14） and “Mingmen”（GV4） for 20 min， once daily for 28 days. After modeling， intraspinal injection of neutralizing antibodies IL-1α， TNF-α and complement 1q （C1q，2 μL） to the injured spinal locus for inhibition of A1 type astrocytes （A1s） was conducted on the 1st， 7th ， 14th and 21st day for rats of AN and EA+AN groups. BBB rating scale was used to evaluate hindlimb locomotor function on day 1， 7， 14， 21 and 28 after modeling. The activation of A2s （its specific marker S100a10）， astrocyte （its specific marker glial fibrillary acidic protein， GFAP）， and A1s （its specific marker C3） in the spinal cord was detected by immunofluorescence， and the protein expressions of NF-200 and NL1 in the spinal cord detected by Western blot and immunohistochemistry， separately， and the neuronal regeneration was observed after Nissl staining.ResultsAfter SCI， the BBB scores at 1 ， 7， 14， 21 and 28 day， and the immunoactivity of NL1 and NF-200 were significantly decreased （P<0.01）， and the fluorescence intensity of double labelled S100a10 （A2s）/GFAP and C3， and the expression of NF-200 were considerably increased in the model group （P<0.05， P<0.01）. In contrast to the model group， the BBB scores at 7， 14， 21 and 28 day， and the immunoactivity of NL1 and NF-200， and the fluorescence intensity of A2s/GFAP in the AN， EA and AN+EA groups， and the expressions of NL1 in the EA and AN+EA groups， and expression of NF-200 protein in the AN+EA group were evidently increased （P<0.05， P<0.01）， and the fluorescence intensity of C3 was strikingly decreased in the EA group （P<0.01）. The effect of AN+EA was significantly superior to that of single AN and EA in increasing BBB scores at 14， 21 and 28 day， and in up-regulating the immunoactivity of NF-200（P<0.01， P<0.05）. Nissl staining showed damaged structure of the gray matter of the spinal cord， atrophy of the Nissl body， and pyknosis of neurons， which was milder in the AN and EA groups， particularly in the AN+EA group.ConclusionEA at GV14 and GV4 may promote activation of A2s and promote regeneration of axons and synapses in SCI model rats.关键词：Spinal cord injury;Electroacupuncture;A2 type astrocyte;Neurofilament protein 200;Nexin 1;Nerve regeneration58|10|2更新时间：2023-09-18
- 摘要：ObjectiveTo observe the anti-tumor effect of moxibustion with seed-sized moxa cones on Hepa1-6 liver cancer bearing （HLCB） mice and its regulatory mechanism on cell apoptosis.MethodsA total of 40 male C57BL/6 mice were randomly divided into control， moxibustion， cyclophosphamide （CTX） and moxibustion+CTX groups， with 10 mice in each group. The HLCB model was established by subcutaneous inoculation of Hepa1-6 cancer cells into the right armpit. Mice of the CTX and moxibustion+CTX groups were given intraperitoneal injection of CTX （30 mg/kg）， once daily for 3 days. Moxibustion with seed-sized moxa cones were applied to “Dazhui” （GV14）， bilateral“Zusanli” （ST36） and “Sanyinjiao” （SP6）， with 5 moxa cones for each acupoint， once daily for 10 consecutive days. The survival status scores and body weight of HLCB mice were observed， and the tumor weight and tumor inhibition rate were detected. HE staining was used to observe the morphological changes of tumor tissue. ELISA was used to detect the levels of serum interleukin （IL）-2， IL-4 and tumor necrosis factor-α （TNF-α）. Western blot and fluorescent quantitative real-time PCR were used to detect the protein and mRNA expressions of cysteine aspartate protease （Caspase） -3 and Caspase-9 in tumor tissues， separately.ResultsCompared with the control group， the survival status scores， body weight， serum IL-2 and TNF-α levels were significantly increased （P<0.05， P<0.01）， the tumor weight and serum IL-4 levels were significantly decreased （P<0.05） in the moxibustion group；while the survival status， body weight， tumor weight， serum IL-2 and IL-4 levels were significantly decreased （P<0.01， P<0.05）， the content of TNF-α was significantly increased （P<0.01） in the CTX group. The protein and mRNA expressions of Caspase-3 and Caspase-9 in the 3 trentment groups were significantly increased （P<0.05， P<0.01）. In comparison with the moxibustion group， the survival status scores， body weight and tumor weight， serum content of IL-2 were significantly decreased （P<0.01， P<0.05）. In contrast to the CTX group， the survival status scores， body weight， serum IL-2 and TNF-α content， and the expressions of Caspase-3 and Caspase-9 in tumor tissue were significantly increased （P<0.01， P<0.05）， and the tumor weight and serum IL-4 content were significantly decreased （P<0.05） in the moxibustion+CTX group. Results of HE staining showed that the tumor cells in the control group had clear nuclear membranes and nucleoli， with more dividing cells； while less nuclear division and an increase in tumor necrosis areas were found in the 3 treatment groups.ConclusionMoxibustion with seed-size moxa cone can enhance the anti-tumor effect of CTX and improve the quality of life of HLCB mice， which may be related with its effect in activating the expressions of Caspase-3 and Caspase-9 in tumor tissue.关键词：Moxibustion with seed-size moxa cone;Cyclophosphamide;Liver cancer bearing mice;Caspase-3;Caspase-935|5|1更新时间：2023-09-18
- 摘要：ObjectiveTo investigate the effects of mild moxibustion at 45°C on the chronic inflammatory response of the abdominal aorta in rats with hyperlipidemia and the effects of different moxibustion durations.MethodsThirty-six SD rats were randomly divided into the following groups： blank control group （2 weeks）， model group （2 weeks）， moxibustion group （2 weeks）， blank group （4 weeks）， model group （4 weeks）， and moxibustion group （4 weeks）. A model of hyperlipidemia with chronic inflammation was established through high-fat diet feeding for 8 weeks. Rats in the moxibustion groups received mild moxibustion treatment at bilateral “Zusanli”（ST36） at 45 °C， 10 min every time， once a day， for consecutive 2 or 4 weeks. The morphology of the abdominal aorta in each group was observed by using HE staining. Contents of serum total cholesterol （TC）， triglycerides （TG）， high-density lipoprotein （HDL）， low-density lipoprotein （LDL）， oxidized low-density lipoprotein （ox-LDL）， intercellular adhesion molecule-1 （ICAM-1）， vascular cell adhesion molecule-1 （VCAM-1）， interleukin 6 （IL-6）， tumor necrosis factor alpha （TNF-α）， endothelin-1 （ET-1） and the contents of nitric oxide （NO）， ox-LDL， and ET-1 in the abdominal aorta were measured by using ELISA. Protein and mRNA expressions of IL-6 and TNF-α in the abdominal aorta of rats in each group were detected by using Western blot and real-time fluorescence quantitative PCR respectively. The positive expression of IL-6 in the abdominal aorta of rats was detected by Immunofluorescence.ResultsCompared to the blank control group， rats in the model group had increased contents of LDL， TC， TG， ox-LDL， VCAM-1， ICAM-1， IL-6， TNF-α， and ET-1 in the serum， increased contents of ox-LDL and ET-1 in the abdominal aorta， increased protein and mRNA expressions of IL-6 and TNF-α in the abdominal aorta（P<0.01，P<0.05，P<0.001）， with decreased HDL content in the serum， decreased NO content in the abdominal aorta （P<0.01，P<0.05）， as well as dark pink abdominal aorta， rough textures in the adventitia， media， and intima， and rough endothelial layer. Compared to the model group（2 weeks）， LDL， ICAM-1， ET-1 contents in the serum， ox-LDL content in the abdominal aorta were decreased（P<0.05）， while serum IL-6 and TNF-α contents， and NO content in the abdominal aorta were significantly increased（P<0.01，P<0.05）， with smoother vascular walls， and relatively clear nucleus and surrounding tissue structures of abdominal aorta in the moxibustion group（2 weeks）. Compared to the model group（4 weeks）， contents of LDL， TC， TG， VCAM-1， ICAM-1， IL-6， TNF-α， ox-LDL， and ET-1 in the serum， ox-LDL and ET-1 contents in abdominal aorta， protein and mRNA expressions of IL-6 and TNF-α in the abdominal aorta were significantly decreased（P<0.05， P<0.01）， while HDL content in the serum and NO content in the abdominal aorta were significantly increased（P<0.05， P<0.01）， with smoother vascular walls， and relatively clear nucleus and surrounding tissue structures of abdominal aorta in the moxibustion group（4 weeks）. In addition， content of HDL in the serum were significantly increased（P<0.05）， while TNF-α content in the serum， protein expression of IL-6 in the abdominal aorta were significantly decreased （P<0.001， P<0.05）， with smoother vascular walls， and clearer nucleus and surrounding tissue structures of abdominal aorta in the moxibustion group（4 weeks）， in comparison with the moxibustion group（2 weeks）.ConclusionMild moxibustion of 45 °C at ST36 can improve vascular endothelial damage and inflammatory response induced by high-fat diet by regulating serum lipids， vascular tone， adhesion molecules， and inflammatory factors， of which the effect of moxibustion intervention for 4 weeks is more significant.关键词：Moxibustion;Hyperlipidemia;Chronic inflammatory response;Abdominal aorta;Moxibustion duration;Zusanli （ST36）30|3|2更新时间：2023-09-18
- 摘要：ObjectiveTo observe the effect of transcutaneous auricular vagus nerve stimulation （taVNS） on the improvement of depressive-like behavior and the splenic α7 nicotinic acetylcholine receptor （α7nAchR） / Janus kinase 2 （JAK2 / signal transducer and activator of transcription 3 （STAT3） signaling pathway in lipopolysaccharide （LPS）-induced depressive-like behavior rats， so as to investigate the antidepressant mechanism of taVNS.MethodsSD rats were randomly divided into SD control group， SD model group and SD taVNS group， and α7nAchR knockout rats were also randomly divided into α7 control group， α7 model group and α7 taVNS group， with 6 rats in each group. Rat model of depressive-like behavior was established by intraperitoneal injection of LPS （1 mg/kg）. Rats in both SD taVNS and α7 taVNS groups received taVNS intervention once a day （2 Hz/15 Hz， 2 mA， 30 min） from 7 days before LPS injection to 2 days after LPS injection， respectively. The mean speed， activity time and side immobility time in the open field test were recorded after taVNS. The contents of interleukin 10 （IL-10） and chemokine （C-X-C motif） ligand 1 （CXCL1） in serum were detected by electrochemiluminescence multifactorial method. The splenic phosphorylated （p）-JAK2 and p-STAT3 protein expressions were detected by Western blot.ResultsCompared with their respective control groups， the mean speed and active time were reduced （P<0.01， P<0.05，P<0.001） and the side immobility time was increased （P<0.001） in the open field test， serum IL-10 and CXCL1 levels were up-regulated （P<0.01， P<0.05，P<0.001）， and splenic p-JAK2 protein expressions were down-regulated （P<0.05，P<0.01） in SD and α7nAchR knockout rats，and splenic p-STAT3 protein expression were down-regulated （P<0.05） in SD rats after LPS injection. Following taVNS intervention and in comparison with the model group ， the mean speed and active time were increased （P<0.01） and the side immobility time was decreased （P<0.001） in the open field test， serum IL-10 and CXCL1 levels down-regulated （P<0.05）， while splenic p-JAK2 and p-STAT3 protein expressions were up-regulated （P<0.01， P<0.001） in the SD taVNS group rather than in the α7 taVNS group. Compared with SD taVNS group， the α7 taVNS group showed increased （P<0.001， P<0.05） side immobility time in the open field test and serum IL-10， decreased splenic p-JAK2 and p-STAT3 protein expressions （P<0.01， P<0.05）.ConclusiontaVNS may exert anti-inflammatory effects through modulating the splenic α7nAchR/JAK2/STAT3 signaling pathway， thereby ameliorating LPS-induced depressive-like behavior in rats.关键词：Transcutaneous auricular vagus nerve stimulation;Depression;Lipopolysaccharide;Inflammation68|8|1更新时间：2023-09-18
- 摘要：ObjectiveTo observe the effect of acupuncture combined with medication on the pregnancy outcome of kidney deficiency and blood stasis type recurrent implantation failure （RIF） in prethrombotic infertility patients.MethodsA total of 70 RIF patients of kidney deficiency and blood stasis type who were to undergo freeze-thaw embryo transplantation were randomly divided into control and treatment groups， with 35 cases in each group. Patients in the control group were given the basic treatment of artificial periodic freeze-thaw embryo transfer and oral aspirin enteric-coated tablet， 25 mg each time， twice a day， for 3 consecutive menstrual cycles. Patients in the treatment group were treated with acupuncture on the basis of the control group， 30 min each time， 3 times a week， for 3 consecutive menstrual cycles. The clinical pregnancy rate， embryo implantation rate and live birth rate of the two groups were compared. Before treatment and 1 day before transplantation， the scores of kidney deficiency and blood stasis symptom were compared. The blood flow pulse index （PI） and resistance index （RI） of the uterus spiral artery were detected by Doppler ultrasound before treatment and 1 day before transplantation. The endometrial thickness was detected 1 day before transplantation. The contents of plasma D-dimer， serum homocysteine （Hcy） and serum thromboxane B2 （TXB2） were detected.ResultsThe clinical pregnancy rate， embryo implantation rate and live birth rate in the treatment group were higher than those in the control group （P<0.05）. After treatment， the scores of kidney deficiency and blood stasis symptom， the levels of plasma D-dimer， serum Hcy and TXB2 ，the PI and RI value in both groups were decreased （P<0.05） compared with those before treatment， and the indexes in the treatment group were decreased （P<0.05） more than those in the control group. There was no significant difference in endometrial thickness between the two groups. In the course of treatments， 7 patients in the control group underwent gastric distension pain， poor appetite， constipation， nausea and other gastrointestinal reactions， while only 2 patients in the treatment group had the above discomfort reactions.ConclusionOn the basis of medication， acupuncture can reduce the serum TXB2 content in RIF patients in prethrombotic state， improve vascular endothelial function， enhance endometrial tolerance， alleviate the symptoms of kidney deficiency and blood stasis， reduce drug adverse reactions， and ultimately improve the pregnancy outcome and increase the rate of embryo implantation.关键词：Repeated planting failure;Prethrombotic state;Acupuncture;Pregnancy rate;Endometrial receptivity;Thromboxane B266|5|0更新时间：2023-09-18
- 摘要：ObjectiveTo compare the clinical effect of “initial scheme” and “improved scheme” of acupuncture-aided anesthesia for patients undergoing thoracoscopic lobectomy.MethodsA retrospective analysis about 2 groups （“initial scheme” and “improved scheme”） of patients （40 cases in each group） who underwent thoracoscopic lobectomy was conducted in the present paper. Patients of the “initial scheme” group received thoracoscopic operation with three incisions under acupuncture-aided anesthesia i.e.， electroacupuncture （EA） stimulation of bilateral Hegu （LI4）， Neiguan （PC6）， Houxi （SI3） and Zhigou （SJ6）， combined with Lidocaine and ropivacaine epidural anesthesia and propofol intravenous anesthesia from January of 2013 to December of 2017. Patients of the "improved scheme" group received thoracoscopic operation with single incision under acupuncture-aided anesthesia by EA， combined with ropivacaine paravertevinal block and lidocaine and remifentanil intravenous anesthesia from August 2018 to August 2021. The incidence of intraoperative deep breathing， resuscitation time， ambulatory rate on day after surgery and postoperative incision pain of the two schemes were compared.ResultsThe incidence of intraoperative deep breathing and the degree of postoperative incision pain were significantly lower （P<0.05）， the postoperative resuscitation time was obviously shorter （P<0.05）， and the ambulatory rate on day after surgery was higher （P<0.05） in the “improved scheme” group than in the “initial scheme” group.ConclusionThe "improved scheme" is better than the “initial scheme” in stabilizing the patient’s breathing during thoracoscopic lobectomy operation， shortening the resuscitation time， and ameliorating the postoperative recovery state and pain reaction， thus being a better technical solution in clinical practice.关键词：Acupuncture anesthesia;Thoracoscopic surgery;Enhanced recovery after surgery;Advanced acupuncture41|4|2更新时间：2023-09-18
- 摘要：Endocannabinoid system （ECS）， which composed of its ligands and receptors， widely distributes in peripheral tissues and nerve system. Through complex regulating mechanisms， ECS exerts a variety of biological functions including analgesia. Its utility in analgesic regulation has attracted extensive attention， and the related achievements have also been transformed into clinical practice. With the deepened understanding of the essence of pain， as well as the advances in research techniques， quantity of research evidences showed that ECS could be regulated by acupuncture treatment and exerted analgesic effect in inflammatory pain， neuropathic pain and other related diseases， and the mechanism had also been revealed gradually. By reviewing literatures related to acupuncture analgesia and ECS， we summarized the effect and mechanism of acupuncture analgesia involved with regulation of ECS， pointed out cannabinoid （CB） 1 receptor and CB2 receptor exerted analgesic effect in central and peripheral neural system through mechanisms of inhibiting neural activation and anti-inflammation， respectively. Therefore， we hope to provide reference and inspiration for relevant research and clinical practice in the future.关键词：Acupuncture;Analgesia;Pain;Endocannabinoid system48|10|0更新时间：2023-09-18
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