最新刊期
卷 49 ， 期 10 ， 2024
- “最新研究发现，针刺能改善卵巢低反应小鼠卵巢功能，抑制细胞凋亡，可能通过调节Hippo通路关键分子发挥作用。”摘要：ObjectiveTo observe the protective effect of acupuncture on ovarian function and its impact on key molecules （Yes associated protein ［YAP］ and transcriptional coactivator with PDZ binding motif ［TAZ］） in the Hippo signaling pathway in poor ovarian response （POR） mice， in order to explore its mechanisms underlying improvement of POR by inhibiting ovarian cell apoptosis.MethodsThe mice with regular motility cycle after screening were randomly divided into normal control， model and acupuncture groups， with 12 mice in each group. The POR model was established by gavage of tripterygium wilfordii polyglycoside suspension （50 mg · kg-1·d-1） for 2 weeks， and the mice of the normal control group was received an equal volume of 0.9% sodium chloride solution by gavage. The mice in the acupuncture group received manual acupuncture stimulation of bilateral “Taichong” （LR3） and “Sanyinjiao” （SP6）， and “Zhongji” （CV3） and “Guanyuan” （CV4）， with the filiform needles retained for 20 min， once daily for successive 10 days. The estrous cycle was determined by using vaginal exfoliated cell smear， and the body mass was detected weekly. The levels of serum anti Mullerian hormone （AMH）， follicle stimulating hormone （FSH）， estradiol （E2） and luteinizing hormone （LH） were measured using enzyme-linked immunosorbent assay （ELISA）. Histopathological changes of the ovarian tissue were observed after H.E. staining， and the apoptosis of ovarian granulosa cells was measured using terminal deoxynucleotidyl transferase （TdT） dUTP Nick-End Labeling （TUNEL） assay. The expression levels of YAP， TAZ， B-cell lymphoma-2 （Bcl-2）， Bcl-2 associated X （Bax）， cysteine aspartic acid specific protease-3 （Caspase-3） mRNAs and proteins were detected using real-time PCR and Western blot， respectively.ResultsCompared with the normal control group， the model group had an increase in the rate of estrous cycle disorder， estrous cycle， serum FSH and LH content， and apoptosis of ovarian granulosa cells， and expression levels of Bax and Caspase-3 mRNAs and p-YAP， Bax and Caspase-3 proteins （P<0.01）， and a decrease in the body mass， number of retrieved oocytes， ovarian wet weight and ovarian index， serum AMH and E2 contents， and the expression levels of YAP， TAZ， Bcl-2 mRNAs and proteins （P<0.01）. After acupuncture intervention， modeling induced increase and decrease of indexes mentioned above were completely reversed （P<0.05， P<0.01）. H.E. staining showed deformed ovarian structure， reduced number of normal developing follicles and increased number of atretic follicles， disordered arrangement of the granulosa cells with fewer hierarchy in the model group， which was improved in the acupuncture group， such as increase in the number and improvement in the shape of normal ovarian follicles and reduction of the atretic follicles.ConclusionAcupuncture intervention can inhibit the apoptosis of ovarian granulosa cells and improve the ovarian function of POR mice， which may be related to its effects in up-regulating the expressions of YAP， TAZ （key molecules of Hippo signaling pathway）.关键词：Acupuncture;Poor ovarian response;Hippo signal pathway;Apoptosis;Sex hormone138|9|0更新时间：2024-10-17
- “最新研究发现，电针“四神聪”“风池”能改善血管性痴呆大鼠认知功能，可能通过调节海马乳酸含量和HIF-1α信号通路抑制炎症反应。”摘要：ObjectiveTo observe the effects of electroacupuncture （EA） stimulation of “Sishencong”（EX-HN1） and “Fengchi”（GB20） on lactate （Lac） content， expression of proline hydroxylase 2 （PHD2）， hypoxia-inducible factor-1α （HIF-1α）/nuclear transcription factor- κB （NF- κB）/NOD-like receptor thermoprotein structural domain-associated protein 3 （NLRP3） signaling pathway， and inflammatory factors in hippocampal tissue of vascular dementia （VD） rats， so as to explore its mechanisms underlying improvement of VD.MethodsMale SD rats screened by Morris water maze tests were randomly divided into blank control， sham-operation， VD model and EA groups （12 rats in each group）. The VD model was replicated using the 4-vessel occlusion （VO） method. EA （2 Hz，1 mA） was applied to EX-HN1 and bilateral GB20 for 30 min， once daily for consecutive 21 days. Morris water maze test was employed to test the rat’s memory learning ability before and after modeling and after the intervention. The hippocampal tissue was sampled for observing histopathologic changes with H.E. staining； and detecting Lac content with colorimetric method， and the contents of tumor necrosis factor-α （TNF-α）， interleukin （IL）-1β， and IL-18 （also in serum） by using ELISA， respectively. The immunoactivity levels of PHD2， HIF-1α， and p-NF-κB p65 in hippocampal tissue were detected by immunohistochemistry， and the expression levels of PHD2， HIF-1α， NF-κB p65， p-NF-κB p65 and NLRP3 proteins in hippocampal tissue were detected by Western blot.ResultsCompared with the blank control and sham-operation groups， the escaping latency， Lac content in hippocampus， the TNF-α， IL-1β， and IL-18 contents in both hippocampus and serum， the immunoactivity of HIF-1α and p-NF-κB p65 and expression levels of HIF-1α， NF-κB p65， p-NF-κB p65， and NLRP3 proteins were significantly increased （P<0.01）， while the number of original platform crossing， and PHD2 immunoactivity and protein expression level were significantly decreased （P<0.05， P<0.01） in the model group. Following EA intervention， modeling induced increase and decrease of the indexes mentioned above were all reversed in the EA group （P<0.05， P<0.01）. H.E. staining showed disordered arrangement of neurons， uneven cytoplasm stain， blurred nucleolus or disappearance of nucleolus， dilated capillaries， many apoptotic bodies and increased inflammatory cells in the hippocampus tissue of the model group， which was improved to a certain extent in the EA group， including relatively regular arrangement of neurons， reduced apoptotic bodies and inflammatory cells， etc. in the hippocampus.ConclusionEA stimulation of EX-HN1 and GB20 can improve the cognitive function in VD rats， which may be related to its functions in reducing Lac content， regulating the expression of HIF-1α pathway related proteins， and inhibiting inflammatory responses in the hippocampus tissue.关键词：Vascular dementia;Electroacupuncture;Lactate;Inflammatory response;Hypoxia-inducible factor-1α136|13|0更新时间：2024-10-17
- “最新研究发现，“醒脑开窍”针刺法可有效抑制脑缺血再灌注损伤大鼠神经元铁死亡，具有神经保护作用。”摘要：ObjectiveTo observe the effect of “Xingnao Kaiqiao” needling on the expression of ferroptosis-related proteins in neurons of rats with cerebral ischemia-reperfusion injury （CIRI）， so as to explore its mechanism underlying improvement of CIRI.MethodsMale Wistar rats were randomly divided into sham operation， model， acupuncture and deferoxamine （DFO） groups， with 18 rats in each group. The CIRI model was established by occlusion of the middle cerebral artery. In the acupuncture group， “Xingnao Kaiqiao” needling was applied to “Shuigou” （GV26）， “Neiguan” （PC6） and “Sanyinjiao”（SP6） for 20 min with electroacupuncture （2 Hz/15 Hz， 1 mA） at PC6 and SP6， twice daily for continuous 3 days. Rats of the DFO group received intraperitoneal injection of iron chelator DFO （0.1 g/kg， once daily）. The severity of neurological impairment （neurological deficit score， 0—5 points， the lower the score， the severer is the neurological impairment） was evaluated by using Zausinger 6-poins scaling method. The cerebral infarct volume was measured after 2，3，5-triphenyltetrazolium chloride （TTC） staining， and the histopathological changes of the ischemic brain tissue were observed after H.E. staining. The mitochondrial structure of the hippocampal neurons on the ischemic side of the brain was observed by using transmission electron microscope. The levels of iron deposition rate （%） in the ischemic penumbra of the brain tissue and hippocampus were observed after Prussian blue staining， and the reactive oxygen species （ROS） content of the cerebral ischemic penumbra was assayed using flow cytometry， and the content of glutathione （GSH） content in the ischemic penumbra was detected by using microplate method. The real-time quantitative PCR was used to detect the expression of glutathione peroxidase 4 （GPX4）， divalent metal transporter 1 （DMT1）， transferrin （TF）， transferrin receptor 1 （TFR1）， and ferroportin 1 （FPN1） mRNAs in the ischemic penumbra， and the Western blot was used to detect the expression of GPX4， DMT1， TF， TFR1， FPN1， and ferritin （FER） proteins in the ischemic penumbra.ResultsCompared with the sham operation group， the neurological deficit score， GSH content， expression of GPX4 and FPN1 mRNAs and proteins were significantly decreased （P<0.01）， while the percentage of cerebral infarct volume， iron deposit rates of the cerebral ischemic penumbra and hippocampus， ROS content， and the expression levels of DMT1， TF， and TFR1 mRNAs and proteins and FER protein were considerably increased （P<0.01） in the model group. In comparison with the model group， the decrease of neurological deficit score， GSH content， expression of GPX4 and FPN1 mRNAs and proteins， and the increase of the percentage of cerebral infarct volume， iron deposit rates of the cerebral ischemic penumbra and hippocampus， ROS content， and the expression levels of DMT1， TF， and TFR1 mRNAs and proteins and FER protein were all reversed in both DFO and acupuncture groups （P<0.01， P<0.05）. The effects of acupuncture were significantly superior to those of DFO in lowering the levels of cerebral cortical and hippocampal iron deposit rates， ROS content and in elevating the expression of GPX4 mRNA and protein （P<0.01， P<0.05）. H.E. staining showed large necrotic cells， disordered arrangement of cells in the cerebral cortex and hippocampus， with hyperchromic nuclei， vacuole-like changes， widening of cellular space， and cell swelling in the model group， which was relatively milder in the cell damage in both acupuncture and DFO groups. In addition， the ultrastructure of cells in the hippocampus showed irregular cellular nuclear morphology， atrophy of some mitochondria in the cytoplasm， partial mitochondrial membrane rupture and edema， and loosening of the ridge structure in the model group， which was milder in the mitochondrial impairment （including reduced number of mitochondria， broken mitochondrial membrane and reduced ridge structure in fewer cells） in the acupuncture group.ConclusionThe “Xingnao Kaiqiao” needling intervention has a neuroprotective effect in CIRI rats， which may be related to its functions in regulating ferroptosis-related targets and iron metabolism in cerebral ischemic penumbra， reducing oxidative stress injury， and suppressing neuronal ferroptosis.关键词：“Xingnao Kaiqiao” needling;Cerebral ischemia reperfusion injury;Ferroptosis;Oxidative stress118|10|0更新时间：2024-10-17
- “最新研究发现，电针“百会”“足三里”可促进脑缺血大鼠血管新生，减轻脑损伤，作用机制与提高HIF/VEGF/Notch信号通路相关因子表达有关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on hypoxia-inducible factor （HIF）/vascular endothelial growth factor （VEGF）/Notch signaling pathway and related factors in rats with cerebral ischemia （CI）， so as to explore its regulatory mechanism underlying improvement of CI by promoting cerebral microangiogenesis.MethodsFifteen male SD rats were randomly selected as the sham surgery （sham） group， while other 85 rats were used to prepare CI model according to the modified Zea-Longa method. The successful CI model rats （n=60） were randomly allocated to model， EA， EA+inhibitor， and inhibitor groups， with 15 rats in each group. EA stimulation （1 Hz/20 Hz， 1—2 mA） was applied to “Baihui”（GV20） and right “Zusanli”（ST36） for 30 min， once a day for 7 days. Rats of the 2 inhibitor groups received intraperitoneal injection of YC-1 （HIF-1α inhibitor， 2.5 mg/kg）， once a day for 7 days. The modified neurological severity scale （mNSS， including the motor ［muscular state， abnormal movement］， sensory ［visual， tactile and proprioceptive］， balance and reflex functions， 0—18 points） was used to assess the rats’ neurological deficit state. The percentage of cerebral infarct volume was measured after 2，3，5-triphenyl tetrazolium chloride （TTC） staining， and pathological changes of the ischemic penumbra cortex were observed after H.E. staining. The immunoactivity of CD34 was determined using immunohistochemistry， followed by calculating the microvascular density （MVD） in the ischemic penumbra cortex， and the expression levels of HIF-1α， VEGF， vascular endothelial growth factor receptor 2 （VEGFR2）， Notch1， and Delta like 4 ligand （DLL4） mRNAs and proteins in the ischemic penumbra of brain tissue were detected using real-time PCR and Western blot， respectively.ResultsIn contrast to the sham group， the mNSS score， percentage of cerebral infarct volume， MVD， and expression levels of HIF-1α， VEGF， VEGFR2， Notch1 and DLL4 proteins and mRNAs were all significantly increased in the model group （P<0.01）. In comparison with the model group， the mNSS score and percentage of cerebral infarct volume were significantly decreased in the EA group （P<0.01）， and increased in the inhibitor group （P<0.01）， and the MVD， and expression levels of HIF-1α， VEGF， VEGFR2， Notch1 and DLL4 proteins and mRNAs were further strikingly increased in the EA group （P<0.01）， but obviously decreased in the inhibitor group （P<0.05， P<0.01）. Comparison between EA and EA+inhibitor groups showed that the effects of EA were basically eliminated in lowering mNSS score and percentage of cerebral infarct volume （P<0.01）， and in up-regulating MVD， and expression levels of HIF-1α， VEGF， VEGFR2， Notch1 and DLL4 mRNAs and proteins （P<0.01）. The levels of mNSS score and percentage of cerebral infarct volume were markedly higher （P<0.01） in the inhibitor group than those in the EA+inhibitor group， while the MVD， expression levels of HIF-1α， VEGF， VEGFR2， Notch1 and DLL4 mRNAs and proteins were significantly lower in the inhibitor group than in the EA+inhibitor group （P<0.01）， suggesting an elimination of EA after administration of HIF-1α inhibitor. H.E. staining showed loosened structure and disordered arrangement of neurons， swollen and vacuolized cells with ruptured nuclei， swollen and deformed microvessels in the ischemic penumbra of the brain tissue in the model group， which was improved in the EA group， including reduction of cellular swelling degree and vacuol-like changes， relatively intact of nuclei， and increase of new capillaries.ConclusionEA of GV20 and ST36 can improve neurological deficit and reduce cerebral infarct volume in CI rats， which may be associated with its functions in promoting angiogenesis in ischemic penumbra area and in up-regulating the activities of HIF/VEGF/Notch signaling pathway and related factors.关键词：Ischemic stroke;Electroacupuncture;HIF/VEGF/Notch signaling pathway;Angiogenesis127|15|0更新时间：2024-10-17
- “最新研究发现，针刺特定穴位能显著降低高血压大鼠血压，可能通过提高血清胃泌素含量和CCKBR表达，抑制钠泵重吸收，促进尿钠排泄。”摘要：ObjectiveTo observe the effect of acupuncture on serum gastrin content and urinary sodium excretion in spontaneously hypertensive rat （SHR）， so as to explore its potential mechanism in the treatment of hypertension.MethodsThirty-two male SHRs were randomly divided into model， hydrochlorothiazide， acupuncture and sham acupuncture groups， with 8 rats in each group， and 8 male Wistar-kyoto rats were taken as the control group. Rats in the hydrochlorothiazide group received gavage of hydrochlorothiazide solution （10 mg·kg-1·d-1）， once daily for 4 weeks. Acupuncture was applied to bilateral “Renying” （ST9） and “Zusanli” （ST36） or non-acupoint on both sides for rats in the acupuncture and sham acupuncture groups， with manual stimulation every 10 minutes for a total of 20 minutes， once a day for a total of 4 weeks. The systolic blood pressure of the tail-artery was measured before and 1， 2， 3， and 4 weeks after the intervention. HE staining was used to observe the pathological changes in renal tissue. Serum gastrin contents were detected using enzyme-linked immunosorbent assay （ELISA）. Urinary sodium content was measured by atomic absorption spectrometry. The expression levels of cholecystokinin B receptor （CCKBR） and Na+/K+-ATPase proteins in renal tissue were detected by Western blot， and the mRNA expression levels of CCKBR and the α1 subunit of Na+/K+- ATPase （ATP1A1） were detected by fluorescence quantitative PCR.ResultsCompared with the control group， the systolic blood pressure of the tail artery in the model group were increased significantly before intervention and at the 1st， 2nd， 3rd and 4th weeks of intervention （P<0.05）. Before intervention， the 24 h urine volume of the model， hydrochlorothiazide， acupuncture and sham acupuncture groups were decreased significantly （P<0.05）. After intervention， the 24 h urine volume and urinary sodium excretion in the model group were significantly decreased （P<0.05）； the expression levels of CCKBR protein and mRNA in renal tissue were significantly decreased （P<0.05）； the expression levels of Na+/K+-ATPase protein and ATP1A1 mRNA were significantly increased （P<0.05）； the glomerulus was mildly congested with a small amount of lymphocyte infiltration. Compared with the model group， the systolic blood pressure of the tail artery in the hydrochlorothiazide and the acupuncture groups were decreased significantly at the 2nd， 3rd and 4th weeks of intervention （P<0.05）； the 24 h urine volume and urinary sodium excretion in the hydrochlorothiazide and the acupuncture groups were significantly increased （P<0.05）； the serum gastrin content and the expression levels of CCKBR protein and mRNA in renal tissue were significantly increased （P<0.05）； the expression levels of Na+/K+-ATPase protein and ATP1A1 mRNA were significantly decreased （P<0.05）； there were no obvious pathological changes in renal tissue. A small number of lymphocyte focal infiltration around blood vessels was observed in the kidney tissue of the sham acupuncture group.ConclusionAcupuncture can significantly reduce the tail artery systolic blood pressure of SHR， which may be related to its effect in increasing serum gastrin content and CCKBR expression， inhibiting sodium pump reabsorption， thus promoting urinary sodium excretion.关键词：Acupuncture;Hypertension;Urinary sodium excretion;Gastrin76|3|0更新时间：2024-10-17
- “最新研究发现，预电针刺激“曲池”“血海”穴位可有效防治荨麻疹，其机制与调节过敏反应和细胞焦亡相关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） preconditioning at “Quchi” （LI11） and “Xuehai” （SP10） in prevention of urticaria.MethodsTwenty-four male SD rats were randomly divided into control， model and preconditioning of EA （Pre-EA） groups （8 rats/group）. The urticaria model was established by intradermal injection of dilute allogeneic antioalbumin serum at the spots of the bilateral symmetry of the spine on the back， and followed by tail venous injection of mixture solution of egg albumin diluent， plus 0.5% Evans blue and normal saline. Ten days before the end of modeling， rats of the pre-EA group received EA stimulation of LI11 and SP10 for 20 min， once a day for 10 consecutive days. The times of rat’s scratching the sensitized skin were recorded. HE staining method was used to observe the pathological changes of skin tissue， and toluidine blue staining method was used to observe the morphology of mast cells （MCs） in the skin， blood， mesentery， and peritoneal fluid， and calculate the degranulation rate. Immunohistochemical stainning was used to detect immunoglobulin E （IgE）， histamine （HIS）， and 5-hydroxytryptamine （5-HT） expressions in subcutaneous tissue. NOD like receptor thermal domain associated protein 3 （NLRP3） inflammasome， apoptosis related granule protein （ASC）， and cysteine aspartate aminotransferase 1 （Caspase-1） protein expression levels in skin tissue were detected by Western blot. The contents of serum interleukin（IL）-1β and IL-18 were detected using ELISA method.ResultsCompared with the control group， the scratching times， amount of Evans blue exudation of the sensitized blue spots， degranulation rate of MCs in skin， blood， mesentery and peritoneal fluid， the expression levels of IgE， HIS， 5-HT in subcutaneous tissue， protein expression levels of NLRP3， ASC， Caspase-1 in skin tissue， and the contents of serum IL-1β and IL-18 were significantly increased （P<0.01， P<0.05） in the model group. In comparison with the model group， the scratching times， amount of Evans blue exudation of the sensitized blue spots， degranulation rate of MCs， the expression levels of IgE， HIS， 5-HT in subcutaneous tissue， protein expression levels of NLRP3， ASC， Caspase-1 in skin tissue， and the contents of serum IL-1β and IL-18 in EA group were significantly decreased （P<0.01， P<0.05）.ConclusionEA preconditioning at LI11 and SP10 can prevent and treat UR by inhibiting inflammatory response， which is related to the regulation of pyroptosis.关键词：Electroacupuncture preconditioning;Urticaria;Inflammatory response;Allergic reaction;Cell pyroptosis;Mast cells55|7|0更新时间：2024-10-17
- “最新研究发现，艾灸能显著改善类风湿关节炎大鼠的滑膜炎性反应，其机制可能与调控GPR43蛋白，恢复Th17/Treg平衡有关。”摘要：ObjectiveTo observe the effects of moxibustion on G protein-coupled receptor 43 （GPR43） and helper T cell 17 （Th17）/regulatory T cell （Treg） balance in rats with adjuvant arthritis （AA）， so as to investigate the partial mechanism of moxibustion therapy on rheumatoid arthritis （RA）.MethodsA total of 24 Wistar rats were randomly divided into a normal group， a model group and a moxibustion group， with 8 rats in each group. The AA rat model was replicated using wind， cold and moisture environmental factors + Freund’s complete adjuvant （CFA） injection method. In the moxibustion group， moxibustion was performed on bilateral “Zusanli” （ST36） and “Shenshu” （BL23） for 20 min/time， once daily， for 21 d. The changes of joint swelling degree （JSD） and arthritis index （AI） were observed in each group of rats. Transmission electron microscopy and HE staining were used to examine changes in the cellular structure of the ankle joint synovial tissue in each group. Western blot analysis was employed to detect the expression levels of GPR43 in the synovial tissue of the ankle joints. Flow cytometry was used to measure the percentages of Treg and Th17 in peripheral blood. ELISA was used to determine the contents of interleukin-10 （IL-10） and transforming growth factor-β 1 （TGF-β1） in serum.ResultsCompared with the normal group， the JSD and AI in the model group increased before and after treatment （P<0.01）， with significant synovial pathological and ultrastruct ural injury observed. Additionally， compared with the normal group， the expression of GPR43 in the synovial tissue decreased （P<0.01）， the Treg percentage in peripheral blood decreased （P<0.01） and the Th17 percentage increased （P<0.01）， serum IL-10 and TGF-β1 contents decreased in the model group （P<0.01）. Compared with the model group， the moxibustion group exhibited a significant reduction in JSD and AI after treatment （P<0.01）， the degree of pathological and ultrastruct ural damage in the synovium decreased， the expression of GPR43 in the synovial tissue increased （P<0.05）， the Treg percentage in peripheral blood increased （P<0.01） and the Th17 percentage decreased （P<0.01）， serum IL-10 and TGF-β1 contents increased （P<0.01）. The relative expression of GPR43 in synovial tissue was positively correlated with the percentage of Treg in peripheral blood （r=0.967， P<0.01）.ConclusionMoxibustion can significantly improve the inflammatory response in the synovial membrane of AA rats. The mechanism may be related to moxibustion restoring the Th17/Treg balance through regulating GPR43.关键词：Moxibustion;G protein-coupled receptor 43;Helper T cell 17/ regulatory T cell balance;Adjuvant arthritis76|3|1更新时间：2024-10-17
- “在抑郁症治疗领域，研究者发现针刺能激活海马AMPK/ULK1通路，促进自噬，改善慢性应激大鼠抑郁样行为。”摘要：ObjectiveTo observe the effect of manual acupuncture stimulation on changes of behavior and hippocampal signaling pathways of AMP-activated kinase （AMPK）/UNC-51-like kinase 1 （ULK1） in rats with chronic unpredictable mild stress （CUMS）-induced depression， so as to explore its molecular mechanism underlying improvement of depression.MethodsThirty-two male SD rats were randomly divided into normal control， model， acupuncture and medication （fluoxetine） groups， with 8 rats in each group. The depression model was established by using CUMS （fasting， water depredation， restraint， swimming in cold water， crowding， stroboscope stimulation， and tail clipping， each of which was used once a week） for 6 weeks. Manual acupuncture stimulation was applied to “Baihui”（GV20） and “Yintang”（EX-HN3） for 10 min， once daily， 6 times a week for 6 weeks before every-day modeling. Rats of the medication group received gavage of fluoxetine （10 mg/kg） once a day for 6 weeks before every-day modeling. The percentage of sucrose preference， and the percentages of open arm times and open arm time in the elevated plus maze experiments were detected. The expression levels of AMPK， phosphorylated （p）-AMPK， ULK1， p-ULK1， mammalian target of rapamycin （mTOR） and p-mTOR proteins in the hippocampus tissue were detected by Western blot， and the expression of autophagy effecting protein （Beclin-1） and the selective autophagy receptors （p62） mRNA in the hippocampal tissue was detected using real-time fluorescence quantitative PCR， respectively.ResultsCompared with the normal group， the percentage of sucrose preference， percentage of times of entering the open arm and percentage of open arm time， the expression levels of p-AMPK /AMPK ratio， p-ULK1 /ULK1 ratio of proteins， and Beclin-1 mRNA were significantly decreased （P<0.01， P<0.05）， and the expression levels of p-mTOR /mTOR ratio and p62 mRNA were significantly increased （P<0.01， P<0.05） in the model group. In comparison with the model group， the percentage of sucrose preference， percentage of open arm times and open arm time， the expression levels of p-AMPK /AMPK ratio， p-ULK1/ULK1 ratio of proteins， and Beclin-1 mRNA were significantly increased in both acupuncture and medication groups （P<0.01， P<0.05）， while the expression levels of p-mTOR /mTOR ratio of proteins， and p62 mRNA were markedly decreased only in the medication group （P<0.05）.ConclusionAcupuncture treatment can alleviate depression-like behavior in CUMS rats， which may be related to its functions in up-regulating AMPK/ULK1 signaling pathway to induce cellular autophagy in the hippocampus.关键词：Acupuncture;Depression;Chronic stress;AMPK/ULK1 signaling pathway;Autophagy88|7|0更新时间：2024-10-17
- “在慢性腹壁疼痛治疗领域，TrP电针疗法和TAP阻滞均能显著缓解疼痛，改善焦虑抑郁情绪并提高生活质量。TrP电针疗法在治疗后3个月临床疗效优于TAP阻滞治疗。”摘要：ObjectiveTo compare the clinical efficacy between trigger-point （TrP） electroacupuncture and transversus abdominis plane （TAP） block in treatment of chronic abdominal wall pain （CAWP）.MethodsA total of 62 CAWP patients were randomly divided into a TrP electroacupuncture group （31 cases， 1 case dropped off） and a TAP block group （31 cases， 1 case dropped off）. Electroacupuncture at trigger points was delivered in the TrP electroacupuncture group， and TAP block was administered under ultrasonic guidance in the TAP block group. Separately， the score of the numerical pain rating scale （NRS） was observed before treatment and in 1 week， 1 month and 3 months after treatment； the scores of the self-rating anxiety scale （SAS） and the self-rating depressive scale （SDS） observed before treatment and in 1 week and 3 months after treatment； and the score of the short form 36 questionnaire （SF-36） was observed before treatment and in 3 months after treatment. The utilization rate of remedial drugs was recorded during follow-up visit. The clinical efficacy was compared.ResultsAt each time point after treatment， NRS score decreased in comparison with that before treatment （P<0.05）， the scores of SAS and SDS 1 week and 3 months after treatment were reduced （P<0.05） and the each item score of SF-36 increased （P<0.05） 3 months after treatment of each group. Compared with the outcomes in the TAP block group， NRS scores were reduced 1 month and 3 months after treatment respectively （P<0.05）， the scores of SAS and SDS decreased （P<0.05） and SF-36 score was elevated （P<0.05） 3 months after treatment in the TrP electroacupuncture group. There was no significant difference in the utilization rate of remedial drugs between the two groups. The clinical efficacy of the TrP electroacupuncture group （96.7%） was superior to that of the TAP block group （83.3%， P<0.05）.ConclusionBoth TrP electroacupuncture and TAP block can markedly relieve pain， attenuate the emotional symptoms of anxiety and depression and improve the quality of life in the patients with chronic abdominal wall pain. The clinical efficacy of TrP electroacupuncture is better than that of TAP block 3 months after treatment.关键词：Chronic abdominal wall pain;Trigger point;Electroacupuncture;Transversus abdominis plane block76|2|0更新时间：2024-10-17
- “最新研究显示，头穴丛刺留针结合上肢智能康复机器人训练，可显著提升脑卒中恢复期患者上肢运动功能和肩关节活动度。”摘要：ObjectiveTo explore the effects of cluster needling at scalp points with needle retaining combined with the training of the upper-limb intelligent rehabilitation robot on shoulder function in stroke patients during convalescence.MethodsNinety stroke patients during convalescence were collected and randomized into scalp point group， robot training group and combined intervention group， with 30 cases each. In the scalp point group， the cluster needling was delivered at scalp points with the needles retained. In the robot training group， the patients were trained with the upper-limb intelligent rehabilitation robot. In the combined intervention group， the patients received both the cluster needling and the training of the upper-limb intelligent rehabilitation robot. In each group， the treatment was given once daily， 5 treatments a week with 2 days rest， for consecutive 4 weeks. Separately， before and after treatment， the score of Fugl-Meyer assessment upper extremity scale （FMA-UE） and the score of activity of daily living （ADL） were evaluated. Using the in-built assessment system of the upper-limb intelligent rehabilitation robot， the range of motion （ROM） of forward flexion， horizontal abduction and horizontal adduction of the affected shoulder joint were evaluated. With surface electromyogram （sEMG）， the sEMG value of the deltoid muscle and the pectoralis major on the affected side were detected.ResultsCompared with the values before treatment， ADL score and FMA-UA score increased in patients of the three groups （P<0.05）， and ROM of forward flexion， horizontal abduction and horizontal adduction of the affected shoulder joint was larger （P<0.05）， the sEMG value of the fasciculi pectoralis major anterior of patients in the combined intervention group was reduced （P<0.05）， and the sEMG of anterior deltoid tract was elevated in the three groups （P<0.05）. When compared with the scalp point group and the robot group， in the combined intervention group， after treatment， ADL score and FMA-UA score were higher （P<0.05）， ROM of forward flexion， horizontal abduction and horizontal adduction of the affected shoulder joint elevated （P<0.05） and sEMG value of the fasciculi pectoralis major anterior reduced （P<0.05）， while which of the anterior deltoid tract was elevated （P<0.05）.ConclusionsThe cluster needling at scalp points with needle retaining， combined with the training of the upper-limb intelligent rehabilitation robot， improves the upper limb motor function and the range of motion of the shoulder joint in stroke patients during convalescence.关键词：Cluster needling at scalp points with needle retaining;Upper-limb intelligent rehabilitation robot;Stroke;Convalescence;Shoulder function95|10|0更新时间：2024-10-17
- “最新研究显示，“升清降浊”法电针可有效改善糖尿病患者胃肠功能障碍，缓解消化道症状，并调节肠道菌群分布。”摘要：ObjectiveTo explore the efficacy of electroacupuncture （EA） based on the “ascending lucidity and descending turbidity” theory in patients with diabetic gastrointestinal dysfunction and its regulatory effect on gut microbiota.MethodsA total of 48 patients with diabetic gastrointestinal dysfunction were randomly divided into the EA group （24 cases， 3 cases dropped-off） and the control group （24 cases， no dropped off）. Both groups received blood glucose control and basic symptomatic treatment. EA （15 Hz/100 Hz） was applied to Zhongwan （CV12）， Tianshu （ST25）， Zhongfu （LU1）， bilateral Zhangmen （LR13）， bilateral Taibai （SP3）， bilateral Taiyuan （LU9）， bilateral Zusanli （ST36）， and bilateral Shangjuxu （ST37） in the EA group. Sham EA was applied to corresponding non-acupoints without electric stimulation in the control group. Both groups received treatment for 30 min per time， once daily， for a total of 5 treatments followed by a 2-day break， over a consecutive period of 2 weeks. Symptoms were evaluated by gastrointestinal symptom rating scale （GSRS） and gastroparesis cardinal symptom index （GCSI） before and after treatment， and 4 weeks after treatment. During the treatment period， fasting blood glucose levels were monitored daily. Stool samples were collected before and after treatment for 16S rDNA sequencing analysis to examine changes in gut microbiota.ResultsCompared with the control group， GSRS and GCSI scores in the EA group were significantly decreased （P<0.001） after treatment and at follow-up at the same time point， and compared with those before treatment， GSRS and GCSI scores in the EA group were significantly decreased after treatment and at follow-up （P<0.001）. After treatment， the 16S rDNA sequencing showed that at the phylum level， the ratio of Proteobacteria in the EA group was higher than that in the control group （FDR<0.05）. At the genus level， a total of 18 different gut microbiota were identified between the two groups. Compared to the control group， patients in the EA group showed a significant decrease in the abundance of Pseudomonas and Alkalibacter （FDR<0.05）， while the abundance of the remaining 16 gut microbiota was significantly increased （FDR<0.05）. Compared to the baseline， the EA group showed a significant up-regulation of Olsenella （FDR<0.05） and a significant down-regulation of Pseudomonas （FDR<0.05） after treatment.ConclusionEA based on the “ascending lucidity and descending turbidity” theory can effectively improve the symptoms of patients with diabetic gastrointestinal dysfunction. EA can also affect the distribution of gut microbiota by up-regulating the levels of certain beneficial bacteria and suppressing pathogenic bacteria .关键词：Diabetic gastrointestinal dysfunction;Electroacupuncture;Gut microbiota;Clinical efficacy98|7|0更新时间：2024-10-17
- “最新研究发现，针刺通过调节大脑前扣带皮层功能，有效改善痛厌恶情绪，减轻疼痛相关负性情绪。”摘要：Pain emotion is a negative emotion caused by pain， which seriously affects the patients’ physical and psychological health. The anterior cingulate cortex （ACC） is a key brain region in the occurrence and regulation of pain and painful emotion. In this paper， we summed up the relevant progress of researches on acupuncture improvement of pain emotion from neurotransmitters and receptors， cellular signaling pathways and neural circuits of ACC， in order to provide a basis for further research. 1） Acupuncture can regulate the long-term potentiation of ACC synaptic transmission by down-regulating the expression of N-methyl-D-aspartic acid receptor （NMDAR）； and up-regulating the expression of μ-， delta- and κ-opioid receptors， neuropeptide S （NPS）/ NPS receptor， and the number of parvalbumin-expressing interneurons to reduce neuronal excitability， relieving pain emotion； 2） Acupuncture can regulate synaptic transmission of ACC neurons by inhibiting protein kinase Mζ （PKMζ）-GluR1 and cAMP/PKA-ERK-CREB signaling pathways， and modulate synaptic plasticity to ameliorate pain emotion； 3） Acupuncture intervention induced inhibition of rostral ACCGlu-thalamic circuit may result in relief of pain emotion but not pain sensation， whereas inhibition of the rostral ACCGlu-ventrolateral periaqueductal gray matter（vlPAG） circuit may relieve pain sensation but not pain emotion. In addition， our team found that in the treatment of painful diseases by acupuncture， the addition of “spirit-regulating” acupoints can not only improve patients’ negative emotion， but also significantly relive patients’ pain sensation.关键词：Anterior cingulate cortex;Acupuncture;Pain emotion;Research progress83|8|0更新时间：2024-10-17
- “最新研究揭示针刺通过调控多个信号通路发挥抗抑郁作用，为针刺治疗抑郁症提供新思路。”摘要：Depression is high in prevalence rate， recurrence rate and disability rate. Acupuncture is effective on depression. The paper reviewed the regulatory effect of acupuncture on five prominent signaling pathways， i.e. mitogen activated protein kinase （MAPK）， Wnt/β-catenin， Notch， adenyl ate cyclase/cyclic adenosine phosphate/protein kinase （AC/cAMP/PKA） and NO/cyclic guanosine monophosphate （cGMP）； and elaborated the structure of each signaling pathway， the association with depression onset， and the relevant research progress of acupuncture in treatment of depression. In view of the regulation of signal pathways， acupuncture can up-regulate AC/cAMP/PKA and extracellular signal-regulated kinase （ERK）， promote the expression of neurotrophic factors or Bcl-2， prevent from and repair neuronal damage， advance neurogenesis and improve synaptic plasticity； it can modulate the Wnt/β-catenin and Notch signal pathways to protect neurons and increase cell proliferation； and down-regulate JNK and up-regulate NO/cGMP signal pathways to inhibit stress that leads to abnormal apoptosis of neurosis and attenuate neurotoxicity and neuronal damage so that anti-depression of acupuncture is obtained. This review provides a new approach to the mechanism research of acupuncture in treatment of depression through regulating the relevant single pathways.关键词：Acupuncture;Depression;Signaling pathway;Molecular mechanism111|16|0更新时间：2024-10-17
- “最新研究揭示针灸促进肠癌术后胃肠功能恢复的选穴规律，为临床治疗提供科学依据。”摘要：ObjectiveTo investigate the selection rules of acupoints for promoting gastrointestinal function recovery after colorectal cancer surgery using complex network technology.MethodsRelevant literatures on acupuncture and moxibustion for promoting gastrointestinal function recovery after colorectal cancer surgery were searched from databases including PubMed， Embase， Cochrane Library， China National Knowledge Infrastructure （CNKI）， Wanfang Data Knowledge Service Platform， VIP Chinese Journal Service Platform， and SinoMed from retrieved to May 9， 2023. Literatures were selected based on inclusion and exclusion criteria， and a database of acupuncture and moxibustion for promoting gastrointestinal function recovery after intestinal cancer surgery was established. Association rule analysis was performed using IBM SPSS Modeler 18.0. Clustering analysis was performed using IBM SPSS Statistics 26.0. Complex network analysis was conducted using Gephi0.9.2.ResultsA total of 255 articles were included， covering 113 acupoints， with a total usage frequency of 1 080 times. The top 5 frequently used acupoints were Zusanli （ST36）， Shangjuxu （ST37）， Tianshu （ST25）， Zhongwan （CV12）， and Neiguan （PC6）. The meridians predominantly used were the Stomach Meridian， Conception Vessel， and Spleen Meridian. Commonly used specific acupoints included lower he-sea points， five shu acupoints， crossing points， and mu-front acupoints. Association rule analysis showed that the acupoint combination with the highest support degree was ST36-ST37 （33.82%）， followed by ST36-ST25 （23.53%）， ST36-CV12 （23.53%）， and ST36-PC6 （22.43%）. Clustering analysis identified 3 effective clusters. Complex network analysis revealed two core groups of acupoints： the back-lower limb prone position group and the abdomen-limb supine position group. Intervention measures primarily involved filiform needle acupuncture and electroacupuncture. Uniform reinforcing-reducing method was mostly used techniques， with strongly associated acupoints being ST37 and ST36； lifting-thrusting and twisting reducing method came secondly， with strongly associated acupoints being ST25 and CV12. Overall， reducing technique was used more frequently than reinforcing technique.ConclusionAcupuncture and moxibustion for promoting gastrointestinal function recovery after intestinal cancer surgery focuses on strengthening and harmonizing the spleen and stomach， cultivating the body’s foundation， and regulating qi flow. The specific acupoints on the Stomach Meridian， Conception Vessel， and Spleen Meridian were mainly selected， and filiform needle acupuncture and uniform reinforcing-reducing technique were mainly used to realize the bidirectional regulation characteristics and advantages of acupuncture.关键词：Postoperation of intestinal cancer;Gastrointestinal function;Acupuncture and moxibustion;Acupoints selection rules;Complex network64|10|0更新时间：2024-10-17
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