最新刊期
卷 49 ， 期 12 ， 2024
- “最新研究发现，电针“足三里”能显著改善慢性心力衰竭小鼠心功能，提高运动耐量，可能通过激活迷走神经调节自主神经平衡。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Zusanli” （ST36） on improving cardiac function in mice with chronic heart failure （CHF）， so as to explore the mechanism of its regulation on the autonomic nervous system.MethodsEighteen C57BL/6J mice were randomly divided into sham-surgery， model， and EA groups， with 6 mice in each group. The model of myocardial ischemia followed by CHF was induced by ligation of the anterior descending branch of the left coronary artery. Two weeks after modeling surgery， EA intervention （0.5 mA， 2 Hz/15 Hz） was performed bilaterally at ST36 in the EA group， 20 min each time， once every other day for 4 weeks. The left ventricular ejection fraction （LVEF）， left ventricular fractional shortening （LVFS）， left ventricular end-systolic diameter （LVESD）， left ventricular end-systolic volume （LVESV）， left ventricular end-diastolic diameter （LVEDD）， and left ventricular end-diastolic volume （LVEDV） were evaluated using echocardiographic imaging. The exercise tolerance was observed in an exhaustive test. Electrocardiograms were recorded using an electrophysiological recording system to analyze heart rate （HR） changes before and after EA， and frequency domain analysis was used to analyze the heart rate variability （HRV）， including low-frequency （LF） component， high-frequency （HF） component， and their ratio （LF/HF）. Immunofluorescence was used to observe the number of choline acetyltransferase （ChAT）+/c-fos+ co-labeled neurons in the dorsal motor nucleus of the vagus （DMV）.ResultsIn terms of cardiac function， compared with the sham-surgery group， the LVEF and LVFS were decreased （P<0.001） and the LVESD， LVEDD， LVESV， and LVEDV were increased （P<0.001， P<0.01） two weeks after modeling of mice in the model group. After 2 weeks of EA intervention， the LVESD and LVESV in the EA group were significantly smaller than those in the model group （P<0.05）. After 4 weeks of intervention， the LVEF and LVFS in the EA group were significantly higher than those in the model group （P<0.05）， and the LVESD and LVESV were significantly reduced （P<0.05， P<0.01）. Compared with the model group， EA significantly improved the exercise tolerance of CHF mice （P<0.05）. Compared with the sham-surgery group， the LF/HF in the model group and EA group were significantly increased （P<0.05） 2 weeks after modeling， indicating autonomic imbalance. After 4 weeks of intervention， the LF/HF in the EA group showed lower trend than that in the model group but the difference was not statistically significant. Additionally， after immediate EA， HR decreased （P<0.05， P<0.01）， HF increased （P<0.01）， and both LF and LF/HF decreased significantly （P<0.05） of mice in the EA group， indicating that EA corrected the autonomic imbalance in CHF mice. The number of ChAT+/c-fos+ co-labeled neurons in the DMV of the EA group was significantly higher （P<0.001） than that in the model group.ConclusionEA at ST36 significantly improved cardiac function and exercise tolerance in CHF mice. The underlying mechanism may be related to the activation of vagus efferent nerve and the regulation of autonomic nerve balance.关键词：Electroacupuncture;“Zusanli” （ST36）;Vagus nerve;Chronic heart failure119|9|0更新时间：2025-01-09
- “最新研究发现，眼针疗法通过调节抗氧化功能轴改善急性脑缺血再灌注损伤大鼠神经元铁死亡。”摘要：ObjectiveTo observe the effect of eye-acupuncture on the antioxidant function axis： System xc（-）-glutathione-glutathione peroxidase 4 （System xc［-］-GSH-GPX4） in the cortical tissue of ischemic penumbra of acute cerebral ischemia-reperfusion injury （CIRI） rats， so as to explore its underlying mechanism in improvement of CIRI by ameliorating the ferroptosis of neurons via antioxidant function axis.MethodsMale SD rats were randomly divided into sham operation， model， eye-acupuncture and GPX4-inhibitor groups， with 15 rats in each group. The CIRI model was replicated by occlusion of the middle cerebral artery and reperfusion for 24 h. For rats of the eye acupuncture group and inhibitor group， manual acupuncture stimulation was applied to bilateral eye-acupuncture-points “Gan”（Liver）， “Shangjiao”（Upper-energizer）， “Shen”（Kidney） and “Xiajiao”（Lower-energizer） of both eyes， 30 min， 12 h and 24 h after modeling， 3 times altogether. For rats of the inhibitor group， intraperitoneal injection of GPX4 inhibitor RSL3 （an activator of ferroptosis） 10 mg/kg （dissolved in 5% DMSO） was conducted 30 min before every acupuncture stimulation. The neurological function was assessed by using Garcia JH scoring method. The brain infarct size was detected after triphenyl tetrazolium chloride （TTC） staining. The ischemic penumbral cortex tissue of the brain was taken for observing morphological changes after H.E. staining， and for observing ultrastructural changes of the mitochondria by using transmission electron microscope. The contents of malondialdehyde （MDA） and GSH were detected by using photocolorimetric method， the content of ferrous ions （Fe2+） detected using spectrophotometry， and the activity of reactive oxygen species （ROS） assayed by ELISA. The expression levels of solute carrier family 7 member 11 （SLC7A11）， solute carrier family 3 member 2 （SLC3A2） and GPX4 proteins of the ischemic penumbral cortex were detected by Western blot， and those of SLC7A11 and GPX4 mRNAs were detected using real-time quantitative PCR.ResultsCompared with the sham operation group， the Garcia JH neurological deficit score， GSH content， and expression levels of SLC7A11， SLC3A2 and GPX4 proteins， and GPX4 and SLC7A11 mRNAs were significantly decreased （P<0.01）， and the contents of Fe2+ and MDA， and ROS activity considerably increased （P<0.01） in the model group. In contrast to the model group， the decreased and increased levels of the above mentioned indexes were reversed in the eye-acupuncture group （P<0.01，P<0.05） but not in the inhibitor group. The therapeutic effects of eye-acupuncture were reduced by GPX4 inhibitor in increasing the levels of Garcia JH neurological deficit score， GSH content， and expression levels of SLC7A11， SLC3A2， and GPX4 proteins， and GPX4 and SLC7A11 mRNAs， as well as in lowering the contents of Fe2+ and MDA and ROS activity（P<0.05， P<0.01）. Results of TTC staining displayed that the brain tissue on the right side showed obvious gray infarct loci in the model group， which was evidently smaller in the eye-acupuncture group， but not in the inhibitor group. H.E. staining displayed disordered arrangement of cells， with shriveled or broken nucleus， and interstitial edema and vacuolation， and a number of large area typical cerebral infarction， and net-like necrotic loci with blurred necrotic lesion boundaries in the model group， which was apparently milder in the eye-acupuncture group. In the inhibitor group， an increased number of cerebral infarction foci， and disordered arrangement and severe injury of cells were found.ConclusionEye-acupuncture can ameliorate ferroptosis in neurons of CIRI rats by modulating System xc（-）-GSH-GPX4 antioxidant function axis.关键词：Eye-acupuncture;Cerebral ischemia-reperfusion injury （CIRI）;Ferroptosis;System xc（-）-GSH-GPX4;Antioxidant function axis;Ischemic penumbra95|10|0更新时间：2025-01-09
- “最新研究发现，电针通过调控miR-19b-3p表达，改善慢性阻塞性肺疾病小鼠肺部炎性反应，为COPD治疗提供新思路。”摘要：ObjectiveTo explore whether electroacupuncture（EA） can increase the expression of suppressor of cytokine signaling （SOCS）3 by affecting the expression of miR-19b-3p， inhibiting the continuous activation of janus kinase （JAK）1 /signal transducer and activator of transcription （STAT）3 signaling pathway， and improve pulmonary inflammation in chronic obstructive pulmonary disease （COPD） mice.MethodsForty mice were randomly divided into normal， COPD model， COPD+EA， and COPD+miR-19b-3p agomir （agomir）+EA groups. The COPD model was simulated by cigarette smoke exposure for 1 h， twice a day for 3 months. After modeling， mice in the COPD+EA group and the COPD+agomir+EA group received EA stimulation of “Feishu” （BL13） and “Zusanli”（ST36） for 30 min， once every other day， for 14 days. The mice of the COPD+agomir+EA group received intranasal drops of miR-19b-3p agomir solution （50 μL） 24 h before every EA intervention. The pulmonary ventilation functions including forced vital capacity （FVC）， forced expiratory volume （FEV） at the 0.05 s and 0.1 s （FEV0.05 and FEV0.1）， FEV0.05/FVC and FEV0.1/FVC were detected using a pulmonary function analysis system. The pathological morphology of lung tissue was observed after H.E. staining. The contents of interleukin（IL）-6， tumor necrosis factor（TNF）-α and IL-1β in the bronchoalveolar lavage fluid were assayed using ELISA. And the expressions of SOCS3， JAK1， STAT3， phosphorylated（p）-JAK1 and p-STAT3 proteins in the lung tissue were detected using Western blot. The expressions of miR-19b-3p， JAK1， STAT3 and SOCS3 mRNA were detected using real-time fluorescence quantitative PCR.ResultsCompared with the normal group， the COPD model group had a significant decrease in the levels of FVC， FEV0.05， FEV0.1， FEV0.05/FVC and FEV0.1/FVC， and the expression levels of SOCS3 mRNA and protein （P<0.001） and a significant increase in the contents of IL-6， TNF-α and IL-1β， and the expression levels of miR-19b-3p， JAK1 and STAT1 mRNAs， and p-JAK/JAK1 and p-STAT3/STAT3 protein expression ratio （P<0.001）. After EA intervention， the decreased and increased levels of all the indexes mentioned above were reversed in the COPD+EA group （P<0.001， P<0.01， P<0.05）. The effect of the EA+agomir were significantly less than EA in up-regulating the levels of FVC， FEV0.05， FEV0.1， FEV0.05/FVC and FEV0.1/FVC， and the expression levels of SOCS3 protein and in down-regulating the contents of IL-6， IL-1β， TNF-α， and expressions of JAK1 and STAT3 mRNA， and p-STAT3/STAT3 （P<0.001， P<0.01， P<0.05）， suggesting that the effects of EA were weakened after intranasal drops of miR-19b-3p agomir. H.E. staining showed thickened alveolar wall， obvious inflammatory cell infiltration， with some ruptured alveoli fused into large vesicles in the model group， slightly dilated alveoli and small amount of inflammatory cell infiltration in the COPD+EA group， and slight alveolar fusion， slight thickening of alveolar wall， and light inflammatory cell infiltration in the COPD+agomir+EA group.ConclusionEA can inhibit the expression of miR-19b-3p， thereby up-regulating SOCS3 expression and inhibiting the overactivation of JAK1/STAT3 signaling， thus reducing lung inflammatory reaction to improve pulmonary function in mice with COPD.关键词：Electroacupuncture;Chronic obstructive pulmonary disease;miR-19b-3p;SOCS3;JAK1/STAT3 signaling pathway85|5|0更新时间：2025-01-09
- “最新研究发现，电针预处理能减轻2型糖尿病大鼠肾脏损伤，可能通过调节PTEN/PI3K信号通路实现。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） preconditioning on podocytes and phosphatase and tension homologous protein （PTEN）/phosphoinositide-3-kinase （PI3K） signaling pathway in rats with type 2 diabetic kidney injury， so as to explore its potential mechanisms.MethodsFifty male Wistar rats were randomly divided into control， model， EA， inhibitor， and sham EA groups， with 10 rats in each group. Diabetes model was established by high-fat and high-sugar diet and intraperitoneal injection of streptozotocin （40 mg/kg）. For rats in the EA group， EA （2 Hz， 1 mA） preconditioning was applied to “Guanyuan” （CV4）， “Zhongwan” （CV12）， and bilateral “Zusanli” （ST36）， “Fenglong” （ST40） for 15 min， once every other day for 8 weeks. And rats in the sham EA group were given acupuncture at the subcutaneous areas of the same acupoints without EA stimulation. Rats of the inhibitor group were given intraperitoneal injection of BPV （HOpic， 0.6 mg/kg， 0.5 mg/mL） combined with the same EA stimulation as EA group once a week for 8 weeks. The blood glucose level of rats was recorded. Urinary albumin （ALB） content in rats was detected by ELISA. The contents of serum urea （Urea）， creatinine （Crea）， total cholesterol （TC） and triglyceride （TG） were detected by automatic biochemical analyzer. HE staining， PAS staining and Masson staining were used to observe the pathological changes of renal tissues. The ultrastructural changes of podocytes were observed by transmission electron microscopy. The relative expression levels of Synaptopodin， microtubule-associated protein light chain 3 Ⅱ（LC3-Ⅱ）， PTEN and PI3K proteins in kidney were detected by Western blot.ResultsCompared with the control group， the blood glucose， content of serum ALB， Urea， Crea， TC， TG and renal PI3K protein expression in the model group were significantly increased （P<0.01）， while the expression levels of renal Synaptopodin， LC3-Ⅱ and PTEN proteins were decreased （P<0.01）. Compared with the model group， the blood glucose， content of serum ALB， Urea， Crea， TC， TG and renal PI3K protein expression in the EA group decreased （P<0.01， P<0.05）， while the expression levels of renal Synaptopodin， LC3-Ⅱ and PTEN proteins increased （P<0.01）. In comparison with the EA group， the blood glucose， ALB， Urea， Crea， TC， TG content and PI3K protein expression level were increased （P<0.05， P<0.01）， while the expression levels of Synaptopodin， LC3-Ⅱ and PTEN proteins were decreased （P<0.01） in the inhibitor group； whereas， the blood glucose， ALB， Urea， TC， TG content and PI3K protein expression level were increased （P<0.05， P<0.01）， while the expression levels of Synaptopodin， LC3-Ⅱ and PTEN proteins were decreased （P<0.01） in the sham EA group. HE staining and PAS staining showed glomerular hypertrophy and glomerular glycogen deposition； and Masson staining displayed an enhancement of glomerular fibrosis； and electron microscope revealed foot process fusion， basement membrane thickening and autophagosomes reduction in the rat’ s kidney of the model， inhibitor and sham EA groups， which were relatively milder in the EA group.ConclusionEA promotes the expression of PTEN gene-encoded protein in the kidneys of type 2 diabetic rats， thereby inhibiting the activation of PI3K， increasing the autophagy level and protecting the podocytes.关键词：Type 2 diabetes;Kidney injury;Electroacupuncture;Podocyte;Autophagy;Phosphatase and tension homologous protein /phosphoinositide-3-kinase pathway72|3|0更新时间：2025-01-09
- “最新研究发现，“疏肝调神”针刺能改善缺血性脑卒中后抑郁大鼠症状，激活AMPK依赖性自噬可能是其机制。”摘要：ObjectiveTo observe the effect of “Shugan Tiaoshen”（soothing the liver and regulating the mental activities） needling on the expressions of AMP-activated protein kinase （AMPK） and autophagy-related proteins in the prefrontal cortex of rats with depression after ischemic stroke （post-stroke depression）， in order to explore its mechanisms underlying improvement of post-stroke depression behaviors.MethodsA total of 60 male SD rats were randomly divided into sham operation （control）， stroke， post-stroke depression， inhibitor， acupuncture， and acupuncture + inhibitor groups. The post-stroke depression model was established by occlusion of the middle cerebral artery and reperfusion （MCAO/R）， combined with chronic unpredictable mild stimulation （CUMS）. Rats in the inhibitor group received bilateral lateral ventricular injection of AMPK inhibitor （0.1 μmol， 10 μL）. Manual acupuncture stimulation was applied to “Baihui” （GV20）， “Yintang” （GV24+）， “Hegu” （LI4）， and “Taichong” （LR3） for the acupuncture group， once daily for 14 d. Rats in the acupuncture + inhibitor group received acupuncture treatment after 3 d of AMPK inhibitor injection. Neurologic impairment was assessed by Longa score. Behavioral assessment of depression was conducted by sucrose preference test and open field test. H.E. staining was used to observe the morphological changes of prefrontal cortex. Western blot was used to detect the protein expression levels of AMPK， p-AMPK， Beclin1， P62 and LC3 in the prefrontal cortex tissue. Transmission electron microscopy （TEM） was used to observe the ultrastructural changes of prefrontal neurons.ResultsCompared with the control group， only the total locomotor distance， the percentage of central area dwell time within 5 min and the p-AMPK/AMPK were significantly decreased （P<0.01） in the stroke group while Longa score was increased （P<0.01）. Compared with the control group， the sucrose preference， total locomotor distance and the percentage of central area dwell time within 5 min， levels of p-AMPK/AMPK， Beclin1 and LC3Ⅱ/Ⅰ were significantly decreased （P<0.01，P<0.05）， while Longa score and P62 expression was significantly up-regulated （P<0.01） in the post-stroke depression group. Compared with the post-stroke depression group， the sucrose preference， total locomotor distance and the percentage of central area dwell time within 5 min， and the levels of p-AMPK/AMPK， Beclin1， and LC3Ⅱ/Ⅰ were considerably increased （P<0.01）， while P62 expression was notably decreased （P<0.05） in the acupuncture group. Comparison between the inhibitor group and the acupuncture+inhibitor group showed that the sucrose preference， total locomotor distance and the percentage of central area dwell time within 5 min， and the expression level of p-AMPK/AMPK were even lower in the former group than those in the latter group （P<0.05， P<0.01）， and the expression level of P62 was higher in the former group than in the latter group （P<0.01）. H.E. staining showed loose arrangement of prefrontal cortex neurons， reduced number， pyknotic and hyperchromatic nuclei， even with degeneration and necrosis of some neurons in the stroke， post-stroke depression and the inhibitor groups， while increase in neuronal number， with relatively complete structure and uniform stain observed in the acupuncture group. Results of TEM showed disrupted neuronal nuclear membrane， reduction in the number of mitochondria with moderate to severe swelling， and decrease or disappearance of the cristae， and transparent matrix with vacuolation in the post-stroke depression group， which was relatively milder （mild swelling， nearly normal structure and more autophagosome forming） in the acupuncture group and acupuncture+inhibitor group.Conclusion“Shugan Tiaoshen” needling can improve the depressive symptoms in post-stroke depression rats， which may be related to its function in activating AMPK-dependent autophagy process.关键词：Post-stroke depression;Acupuncture;Autophagy;AMP-activated protein kinase79|5|1更新时间：2025-01-09
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Effect of electroacupuncture on blood-brain barrier and central inflammatory response in obese rats AI导读
“最新研究发现，电针治疗能改善肥胖引起的中枢炎性反应，修复血脑屏障，为肥胖治疗提供新途径。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on central nervous inflammatory response in obese rats， so as to explore its mechanisms underlying improvement of obesity.MethodsMale SD rats were randomly allocated to normal control， model， EA and sham-EA groups （n=15/group）. Rats of the normal group were fed with normal diet， and the obesity model was established by feeding the rats with high-fat diet for 8 weeks. EA （2 Hz， 1 mA） was applied to “Zusanli”（ST36）， “Zhongwan”（CV12）， “Guanyuan”（CV4） and “Fenglong”（ST40）， and non-acupuncture points （about 5 mm left to CV4 and CV12 and about 5 mm lateral to ST36 and ST40） for 15 min， once every other day， 3 times a week for 8 weeks （no electrical current was delivered to the non-acupoints）. The body weight and anal-nose length in each group were recorded before and after the intervention and Lee’s index was calculated. The adipocyte morphology of epididymis was detected after H.E. staining， and the contents of serum tumor necrosis factor α （TNF-α）， interleukin （IL）-6， IL-10 and free fatty acid （NEFA） were detected by using enzyme-linked immunosorbent assay （ELISA）. The expression levels of TNF-α， IL-6， IL-10 and inducible nitric oxide synthase （iNOS） proteins in the hypothalamus tissue were detected by Western blot， and the immunoactivity of ionized calcium-binding adapter molecule 1 （Iba-1） in hypothalamic microglia was detected by immunohistochemistry. The content of Evans blue in the brain tissue samples was detected by Evans blue assay to evaluate the integrality of blood-brain barrier.ResultsCompared with the normal group， the body mass （BM）， Lee’s index， contents of serum TNF-α， IL-6 and NEFA， expression levels of hypothalamic TNF-α， IL-6 and iNOS proteins， the immunoactivity level of Iba-1 and the content of Evans blue were significantly increased （P<0.01，P<0.05）， while the content of serum IL-10 and the expression of hypothalamic IL-10 protein considerably decreased （P<0.01） in the model group. After the intervention， modeling induced increase of BM， Lee’s index， contents of serum TNF-α， IL-6 and NEFA， expression levels of hypothalamic TNF-α， IL-6 and iNOS proteins， the immunoactivity level of Iba-1 and the content of Evans blue， and decrease of serum content and expression level of IL-10 in the hypothalamus were reversed by EA stimulation （P<0.01， P<0.05） but not by non-acupoint stimulation. H.E. staining showed that the adipocytes of epididymis became larger in size and decreased in the number in the model group relevant to the normal group， and became smaller after EA （not non-acupoint） relevant to the model group.ConclusionEA can treat obesity， improve body inflammation caused by obesity， and relieve central inflammation by repairing the blood-brain barrier.关键词：Electroacupuncture;Obesity;Blood-brain barrier;Central inflammation72|4|0更新时间：2025-01-09 - “最新研究发现，电针能改善抑郁大鼠行为，促进海马神经元再生，可能通过同步调控Wnt/β-catenin与Notch信号通路实现。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the proliferation of hippocampal neurons and Wnt/β-catenin and Notch signaling pathways in depression like behavior rats， so as to explore its mechanism underlying improvement of depression.MethodsSD rats were randomly divided into blank， model， medication and EA groups， with 12 rats in each group. The depression model was established by chronic unpredictable mild stimulation combined with solitary raising for 35 consecutive days. After 14 days modeling， EA was applied to “Baihui”（GV20） and “Taichong”（LR3） for 15 min once daily for 3 weeks. For rats of the medication group， clomipramine （2.5 mg/kg） was given （i.p.） once a day for 3 weeks. The depression-like behavior was evaluated by open field test and forced swimming test. The ultrastructure of hippocampal neurons was observed by transmission electron microscope （TEM）. The positive expression of 5-bromo-2’-deoxyuridine/neuron-specific nucleoprotein （BrdU/NeuN） in hippocampal dentate gyrus of rats was detected by immunofluorescence double labeling method. Western blot was used to detect the relative expression of Wnt/β-catenin and Notch pathway key proteins β-catenin， G1/S-specific cyclin-D1 （Cyclin D1）， NICD and Hes1 in hippocampus of rats.ResultsCompared with the blank group， the total distance and average speed of open field movement were decreased （P<0.01）， and the immobility time of forced swimming was increased （P<0.01）. The positive expression of BrdU/NeuN in hippocampal dentate gyrus was decreased （P<0.01）. The relative expression of β-catenin and Cyclin D1 protein in hippocampus were decreased （P<0.01）， while the relative expression of NICD and Hes1 protein increased （P<0.01） in the model group. Following the treatment and compared with the model group， the total distance and average speed of movement in the open field were increased （P<0.05）， and the immobility time of forced swimming was decreased （P<0.05） in both medication and EA groups. The positive expression of BrdU/NeuN in hippocampal dentate gyrus was increased （P<0.01）. The relative expression of β-catenin and Cyclin D1 protein in hippocampus were increased （P<0.01）， while the relative expression of NICD and Hes1 protein were decreased （P<0.01， P<0.05） in both medication and EA groups. TEM microscope observation showed irregularity of hippocampal neurons， rupture of nuclear membranes， disruption of mitochondrial structure， and dilation of endoplasmic reticulum in the model group， which was relatively milder in both medication and EA groups.ConclusionEA can improve the depressive state of depression model rats， which may be associated with its functions in regulating Wnt and Notch signaling pathways， thereby promoting the regeneration of hippocampal neurons.关键词：Depression;Electroacupuncture;Neuronal regeneration;Wnt;Notch80|6|0更新时间：2025-01-09
- “电针通过下调HK2介导的Warburg效应，抑制大鼠急性肺损伤炎性反应及肺水肿。”摘要：ObjectiveTo observe whether electroacupuncture （EA） can inhibit the inflammatory response via down-regulating hexokinase 2 （HK2） mediated Warburg effect in rats with acute lung injury （ALI）.MethodsMale Sprague-Dawley rats were randomly divided into control， model and EA groups， with 12 rats in each group. The ALI model was established by injection of lipopolysaccharides （LPS， 5 mg/mL， 1 mL/kg） into the tail vein. The rats in the control group received intravenous injection of the same volume of 0.9% NaCl solution. Immediately after the modeling was completed， EA （3 Hz/15 Hz， 1 mA） was applied to bilateral “Zusanli” （ST36） and “Chize” （LU5） for 30 min， once a day for 5 consecutive days. The contents of serum interleukin-1β （IL-1β） and transforming growth factor-β （TGF-β） were measured by using the enzyme-linked immunosorbent assay （ELISA）， and the wet/dry weight ratio （W/D） of the lung tissue was calculated to assess the severity of pulmonary edema. Histopathological changes of the lung tissue （inflammatory damage， damage score） were observed and calculated after hematoxylin and eosin （H.E.） staining. The qRT-PCR and Western blot were used to detect the expression of HK2 mRNA and protein， respectively. The lactate kit was used to detect the lactate content of the lung tissue.ResultsCompared with the control group， the serum IL-1β content， lung damage score， ratio of W/D of the lung tissue， expression levels of HK2 mRNA and protein and the lactate content were significantly increased （P<0.01）， while the serum TGF-β content was significantly decreased （P<0.01） in the model group. In comparison with the model group， the serum IL-1β content， pathological score， HK2 mRNA expression level， and lactic acid content were significantly decreased （P<0.01）， and the TGF-β content was significantly increased （P<0.01） in the EA group. H.E. stain showed obvious congestion and hemorrhage of the lung tissue， disappearance of the alveolar structure with thickened alveolar wall， and a large number of inflammatory cells infiltration in the model group， which was relatively milder in the EA group， including milder pulmonary tissue congestion， partial alveolar fusion， and fewer inflammatory cells gathered in the alveolar septum， etc.ConclusionEA can reduce the inflammatory response and pulmonary edema of ALI in rats， which may be related to its functions in down-regulating the content of serum IL-1β， and lung HK2 mRNA expression and lactic acid content and in up-regulating serum TGF-β （anti-inflammatory factor） level and HK2-mediated Warburg effect.关键词：Electroacupuncture;Acute lung injury;Hexokinase 2 （HK2）;Warburg effect60|1|0更新时间：2025-01-09
- “最新研究发现，艾灸“肾俞”“足三里”能减轻类风湿性关节炎大鼠滑膜炎性损伤，作用机制可能与降低脂质过氧化及活性氧水平，抑制铁死亡的发生有关。”摘要：ObjectiveTo explore the mechanism of moxibustion in improving synovitis inflammatory injury in rheumatoid arthritis （RA） model rats based on the ferroptosis-lipid metabolism pathway.MethodsForty-eight SD rats were randomly divided into normal， model， moxibustion and moxibustion + long-chain acyl-CoA synthetase 4 （ACSL4） inhibitor groups， with 12 rats in each group. The RA model was replicated using environmental factors of wind， cold， and dampness combined with Freund’s complete adjuvant injection. The moxibustion group received moxibustion at “Shenshu” （BL23） and “Zusanli” （ST36） for 20 minutes per acupoint each time， once daily at a single acupoint （both sides） with alternating acupoints over 15 consecutive days. The moxibustion + ACSL4 inhibitor group received intraperitoneal injection of the ACSL4 inhibitor rosiglitazone （0.4 mg/kg） after moxibustion， once daily for 15 consecutive days. Histopathological changes in synovial tissue were observed using HE staining； serum contents of glutathione （GSH） and malondialdehyde （MDA） were detected using biochemical methods； reactive oxygen species （ROS） levels in synovial tissues were detected using flow cytometry； the expressions of glutathione peroxidase 4 （GPX4）， ACSL4， and lysophosphatidylcholine acyltransferase 3 （LPCAT3） proteins in synovial tissues were detected using Western blot； and serum contents of interleukin （IL）-6 and tumor necrosis factor-alpha （TNF-α） were detected using ELISA.ResultsCompared with the normal group， the serum contents of GSH decreased （P<0.01） while MDA， IL-6， and TNF-α contents increased （P<0.01）； the ROS levels， ACSL4 and LPCAT3 protein expressions increased （P<0.01） while GPX4 protein expression decreased （P<0.01） in synovial tissue in the model group. Compared with the model group， the serum contents of GSH increased （P<0.01） while MDA， IL-6， and TNF-α contents decreased （P<0.01）； the ROS levels， ACSL4 and LPCAT3 protein expressions decreased （P<0.01） while GPX4 protein expression increased （P<0.01） in synovial tissue in the moxibustion group and moxibustion + ACSL4 inhibitor group. Compared with the moxibustion group， the serum contents of GSH increased （P<0.01） while MDA， IL-6， and TNF-α contents decreased （P<0.01）； the ROS levels， ACSL4 and LPCAT3 protein expressions decreased （P<0.01， P<0.05） while GPX4 protein expression increased （P<0.05） in synovial tissue in the moxibustion + ACSL4 inhibitor group. HE staining showed that the model group had significantly increased synovial hyperplasia and inflammatory cell infiltration； the moxibustion group and moxibustion + ACSL4 inhibitor group showed varying degrees of alleviation in inflammatory cell infiltration and hyperplasia in synovial tissue； compared with the moxibustion group， the moxibustion + ACSL4 inhibitor group showed more significant improvements in inflammatory infiltration and hyperplasia of synovial cells， reduced layers of synovium.ConclusionMoxibustion at BL23 and ST36 can alleviate synovial inflammatory injury， and its mechanism may be related to reducing lipid peroxidation and ROS levels， and inhibiting the occurrence of ferroptosis.关键词：Moxibustion;Rheumatoid arthritis;ACSL4;Ferroptosis;Lipid metabolism86|4|0更新时间：2025-01-09
- “最新研究显示，贺氏火针联合核桃壳灸治疗围绝经期干眼效果显著，优于传统滴眼液，有效缓解眼部症状，增加泪液分泌，延长泪膜破裂时间，并改善全身症状。”摘要：ObjectiveTo observe the clinical effect on perimenopausal dry eye disease treated with He’s fire needle therapy combined with walnut-shell moxibustion.MethodsA total of 60 patients with perimenopausal dry eye disease were randomized into an observation group （30 cases） and a control group （30 cases）. In the observation group， the pricking technique with fire needle was operated at bilateral Naokong （GB19）， Feishu （BL13）， Xinshu （BL15）， Ganshu （BL18）， Pishu （BL20）， Shenshu （BL23）， 3 times a week， for 4 weeks， and the walnut-shell moxibustion was combined， 30 minutes each time， 3 times a week， for 4 weeks. In the control group， hyaluronic acid sodium eye drops were used， one drop each time， 3 times a day， for 4 weeks. Before and after treatment， the scores of the questionnaire for dry eye disease in China， the result of lacrimal secretion test （Schimer Ⅰ test， SⅠT） and tear film break-up time（BUT）， as well as the modified Kupperman score were evaluated in the two groups. The clinical effect was compared between the two groups.ResultsAfter 4 weeks of treatment， the results of SⅠT and BUT increased （P<0.001）， while the scores of the questionnaire for dry eye disease in China， the modified Kupperman score decreased （P<0.001） in the two groups when compared with the baseline. In the observation group， the result of SⅠT and BUT were higher than those of the control group （P<0.05）， while the scores of the questionnaire for dry eye disease in China， and the modified Kupperman score was lower （P<0.05）. The marked effective rate （30/60， 50.0%） in the observation group was better than that （10/60， 16.7%） of the control group （P<0.05）.ConclusionIn treatment of perimenopausal dry eye disease， the combined therapy of fire needling and walnut-shell moxibustion obtains the superior effect to hyaluronic acid sodium eye drops. This combined therapy can effectively relieve the eye symptoms， increase the secretion of tears， prolong BUT， and ameliorate systemic symptoms.关键词：Dry eye disease;Perimenopause;Walnut-shell moxibustion;Fire needle therapy;Naokong （GB19）;Back-shu points of five zang organs88|10|0更新时间：2025-01-09
- “在医疗领域，现代针刺麻醉技术展现了其独特价值，为围术期管理提供了中国特色的解决方案。”摘要：Acupuncture anesthesia is an innovative anesthesia technique which combines acupuncture therapy of traditional Chinese medicine with western anesthesia and surgery. Modern acupuncture anesthesia technology involves comprehensive preoperative evaluation and application， intraoperative assistance in anesthesia， and enhanced recovery after surgery， forming a set of perioperative management models with Chinese characteristics. In the present paper， we comprehensively summarized the application scenarios （including craniocerebral operations， neck surgery， cardiac and thoracic surgery， abdominal operation， obstetrical and gynecological operations， anorectal operation， and orthopedic operation） and values （such as analgesia， reduction in nausea and vomiting， accelerating recovery of gastrointestinal function， regulating immune function， etc.） of modern acupuncture anesthesia technology in the perioperative period， and also analyzed its advantages ［（1） fewer physiological interference to the patient， reducing operation-induced organ damage， and accelerating postoperative recovery； （2） no obvious adverse reactions and high safety； （3） significant social and economic benefits］， current challenges and opportunities of modern acupuncture anesthesia techniques. We think that it is urgent to formulate a set of consensus guidelines and reference for modern acupuncture anesthesia for each type of relevant operations based on evidence-based medicine， actively draw on， absorb and introduce the latest evidence-based concepts and research methodologies and technologies， strive to create new theories， new methods and new technologies， carry out standardized sustainable research， enrich and improve its own system and to expand its application scope through exchanges and mutual learning with cutting-edge technologies of modern medicine.关键词：Modern acupuncture anesthesia;Clinical practice;Multidisciplinary intersection;Enhanced recovery after surgery;Translational medicine149|14|0更新时间：2025-01-09
- “最新研究揭示小胶质细胞在缺血性脑卒中治疗中的重要作用，针刺干预有望成为新疗法。”摘要：Microglia， the main phagocytic cells in the brain， play a key role in inflammatory response and neuroprotection in ischemic stroke， and are potential targets for the treatment of ischemic stroke. Multiple studies have confirmed that the effect of acupuncture on ischemic stroke is closely related to its effect in regulating the activities of microglia. In the present paper， we reviewed the role of microglia in ischemic stroke and the current research status of acupuncture intervention， and prospects the future research direction. Past researches showed that microglia have a bidirectional role in the pathological process of cerebral ischemia， that is， M1 microglia can secrete pro-inflammatory cytokines， participate in the damage of the blood-brain barrier， and aggravate the damage of neurotoxicity， and M2 microglia can secrete anti-inflammatory cytokines， nerve growth factors， neurotrophic factors， improve neuroplasticity and promote vascular remodeling. Acupuncture can exert brain protective effects by regulating the activation and polarization direction of microglia， inhibiting inflammatory reactions， maintaining the integrity of the blood-brain barrier， promoting vascular remodeling， and promoting nerve regeneration. However， whether acupuncture intervention in different periods can affect the response of microglia and how it affects， and whether different acupuncture parameters have different effects on the regulation of microglia， it needs further research. In addition， more in-depth and multi-angle systematic studies on polarization of microglia， secretion factors， cell relationships， upstream and downstream regulatory factors and other aspects with microglia are definitely needed.关键词：Acupuncture;Ischemic stroke;Microglia;Review95|7|0更新时间：2025-01-09
- “最新研究发现，针刺通过调节细胞程序性死亡通路，有望成为治疗脊髓损伤的新疗法。”摘要：Spinal cord injury （SCI） is a highly disabling disease of the central nervous system， and seriously affects the patients’ quality of life. A large number of studies have shown that programmed cell death （PCD） is an important pathological mechanism of secondary injury in SCI. As a non-drug therapy， acupuncture therapy has the characteristics of bidirectional， holistic and multi-level actions， which can target PCD pathways to play a neuroprotective effect， and has great potential in regulating PCD in the treatment of SCI. In the present paper， we systematically elaborated the PCD pathway related to SCI and summed up the possible mechanism of acupuncture underlying improvement of SCI from 1） regulating apoptosis， 2） modulating autophagy， 3） regulating pyroptosis， 4） inhibiting ferroptosis， and 5） suppressing necroptosis. These may provide a theoretical basis for acupuncture treatment of SCI and for further research in the coming years.关键词：Spinal cord injury;Acupuncture;Programmed cell death;Review59|2|0更新时间：2025-01-09
- “最新研究发现，针灸治疗帕金森病重用合穴及原穴，以脏腑经络辨证为基础，为临床治疗提供新思路。”摘要：ObjectiveTo analyze the regularity of acupoint selection and compatibility of acupuncture and moxibustion for Parkinson’s disease （PD） using complex network analysis technology， so as to provide the basis and treatment strategies for clinical acupuncture and moxibustion treatment of PD.MethodsRelevant literature published in databases of China National Knowledge Infrastructure （CNKI）， Wanfang， VIP， PubMed， and Web of Science from the inception to December of 2023 was retrieved. The literature was screened based on inclusion and exclusion criteria to establish a prescription database for acupuncture treatment of PD. Excel was used to analyze the frequency of use of acupoints， meridian normalization and specific acupoints. The IBM SPSS Modeler18.0 was used for modeling and association rule analysis， while the Gephi0.10.1 was utilized for complex network analysis.ResultsA total of 326 valid articles were included， from which 496 valid acupuncture prescriptions were extracted， involving 251 acupoints with a total usage frequency of 3，968 times. The top 5 most frequently used acupoints were Baihui （GV20， 225 times）， Taichong （LR3， 215 times）， Fengchi （GB20， 202 times）， Hegu （LI4， 192 times）， and Sanyinjiao （SP6， 134 times）. The second-order and third-order association rules between the acupoints indicated that the combinations of LI4-LR3 and GB20-LR3-LI4 had the highest support. After analyzing the complex network topology， 27 core acupoints for the treatment of PD were identified. Commonly used meridians are Governer Vessel， Gallbladder Meridian of Foot Shaoyang ， Stomach Meridian of Foot Yangming. He-sea acupoints and yuan-source acupoints are the majority in the specific acupoints. The community analysis of the acupoints revealed 3 groups： the head acupoints group of the Governor Vessel， the acupoints group for nourishing the liver and kidney， and the acupoints group for expelling pathogens and purging excessiveness.ConclusionAcupuncture and moxibustion treatment for PD often emphasizes the use of he-sea acupoints and yuan-source acupoints， and focus on combination of upper and lower acupoints， as well as the selection of acupoints within the same meridian. The treatment is based on zang-fu and meridian differentiation， with selection of the Governor Vessel to calm the mind and compatibility with acupoints to regulate the liver and tonify the kidney for the root causes， while utilizing limb acupoints to dispel wind， resolve phlegm， eliminate dampness， and purge heat for the symptoms.关键词：Acupuncture and moxibustion therapy;Parkinson’s disease;Acupoint compatibility;Complex network analysis;Literature study75|14|0更新时间：2025-01-09
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