最新刊期
卷 49 ， 期 2 ， 2024
- 摘要：ObjectiveTo observe the analgesic effects of different levels and intensities of electrical stimulation on the local acupoints in the pain source area and their impact on wide dynamic range （WDR） neurons in the spinal dorsal horn， in order to provide a basis for selecting appropriate parameters for electroacupuncture （EA） stimulation.MethodsWistar rats were used in 3 parts of the experiment. Complete Freund’s adjuvant was used to establish a model of inflammation-induced pain in the gastrocnemius muscle. After modeling， 6 rats were randomly selected for multi-channel extracellular electrophysiological recording of the electrical activity of WDR neurons， to determine the threshold for activating the A-component （Ta） and the C-component （Tc）， which were used as the intervention intensities for skin transcutaneous electrical acupoint stimulation （TEAS） or EA. Thirty-six rats were randomly divided into normal ， model ， TEAS-Ta ， TEAS-Tc， EA-Ta ， and EA-Tc groups， with 6 rats in each group. In the pain source area ，Ta or Tc intensity of TEAS or EA intervention at“Chengshan”（BL57） was performed for 30 min each time， once a day， for 3 consecutive days. A small animal pressure pain measurement instrument was used to measure the mechanical pressure pain threshold of the gastrocnemius muscle in rats， and the Von Frey filament was used to measure the mechanical pain threshold of the footpad. Thirteen rats were randomly selected to observe the immediate responsiveness of WDR neurons to Ta/Tc intensity of EA or TEAS in BL57.ResultsThe thresholds of TEAS to activate WDR neuron A-component or C-component were （2.43±0.57） mA and （7.00±1.34） mA， respectively， while the thresholds for EA to activate muscle WDR neuron A-component or C-component were （0.72±0.34） mA and （1.58±0.35） mA， respectively. After injection of CFA into the gastrocnemius muscle， compared with the normal group both the mechanical pressure pain threshold of the gastrocnemius muscle and the mechanical pain threshold of the footpad of rats in the model group were significantly decreased （P<0.001）. After TEAS-Ta， TEAS-Tc or EA-Ta intervention in the BL57， both the mechanical pressure pain threshold of the gastrocnemius muscle and the mechanical pain threshold of the footpad were significantly higher than those in the model group （P<0.05， P<0.001）. Compared with the normal group， the electrical threshold for evoking WDR neuron C-component discharge was significantly decreased （P<0.001） in the model group，while increased after TEAS-Ta， TEAS-Tc， or EA-Ta intervention （P<0.01） compared with the model group. The evoked discharge frequency of muscle WDR neurons decreased significantly after immediate intervention with TEAS-Ta， TEAS-Tc， or EA-Ta （P<0.01，P<0.05）. EA-Tc had no significant improvement on the evoked electrical activity of WDR neurons or pain behavior.ConclusionTEAS-Ta， TEAS-Tc， or EA-Ta can all alleviate the local and footpad mechanical pain in rats with muscle inflammation and inhibit the responsiveness of WDR neurons， indicating that different intensities are required for analgesic effects at different levels of acupoints in the pain source area.关键词：Electroacupuncture;Stimulation intensity;Stimulation layer;Transcutaneous electrical acupoint stimulation;Wide dynamic range neurons;Inflammatory muscle pain97|10|0更新时间：2024-02-21
- 摘要：ObjectiveTo observe the effect of electroacupuncture （EA） stimulation of “Jiaji”（EX-B2） on motor function， histomorphology， and expression of NOD-like receptor protein 3 （NLRP3） and N-terminal domain of gasdermin D （GSDMD-N） in the spinal cord tissue of rats with spinal cord injury （SCI）， so as to explore its mechanism underlying improvement of SCI.MethodsForty eight female SD rats were randomly divided into sham surgery （sham）， SCI model （model）， EA， and NLRP3 agonist （monosodium urate， MSU） combined with Jiaji EA （MSU+EA） groups， with 12 rats in each group which were further divided into 3 d and 7 d subgroups， with 6 rats at each time point. Two EA groups received EA stimulation of EX-B2 with a frequency of 100 Hz， electrical current of 1—2 mA for 30 min， once a day for 3 or 7 days. After 5 min， 6 h， and 24 h of modeling， rats of the MSU+EA group received intraperitoneal injection of MSU （200 μg/kg， 200 μg/mL） . The motor function was evaluated using Basso-Beattie-Bresnahan （BBB） scale， the morphological structure of rat spinal cord tissue was observed by H.E. staining. The expression of pyroptosis related factors NLRP3， cleaved Caspase-1 and GSDMD-N of the spinal cord was observed by using immunohistochemistry and Western blot separately， the expression and localization of Iba-1 and GSDMD-N in the spinal cord tissue were observed using immunofluorescence double staining method.ResultsCompared with the sham group， the BBB scores after modeling and on day 3 and 7 were decreased （P<0.05）， while the average OD values （immunoactivity） and expression levels of NLRP3， cleaved Caspase-1 and GSDMD-N proteins， and the immunofluorescence intensity of Iba-1/GSDMD-N （co-expression） of the spinal cord tissues on day 3 and 7 were significantly increased in the model group （P<0.05）. In comparison with the model group， the BBB scores on day 3 and 7 were obviously increased （P<0.05）， while the immunoactivity and expression levels of NLRP3， cleaved Caspase-1 and GSDMD proteins， and the immunofluorescence intensity of Iba-1/GSDMD-N on day 3 and 7 significantly down-regulated in the EA group （P<0.05） but not in the MSU+EA group （P>0.05）， suggesting an elimination of the effects of EA after administration of NLRP3 agonist （MSU）. H.E. staining showed obvious bleeding area in the spinal cord tissue， loose tissue and inflammatory cell infiltration on day 3 after modeling， and basic absorption of the bleeding， loose tissue， obvious vacuolar changes of the white matter area， loss and contraction of neurons with infiltration of a large number of inflammatory cells， which was milder in the EA group but not in the MSU+EA group.ConclusionEA of EX-B2 can improve the motor function of SCI rats， which may be related to its functions in inhibiting pyroptosis of microglia mediated by NLRP3/Caspase-1 signaling pathway.关键词：Electroacupuncture;Jiaji （EX-B2）;Spinal cord injury;NLRP3;Pyroptosis;Microglia72|8|3更新时间：2024-02-21
- 摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Dazhui” （GV14） and “Jizhong”（GV6） of the Governor Vessel （GV） on mitochondrial fusion and neural stem cell （NSC） proliferation and differentiation in the spinal cord of rats with spinal cord injury （SCI）， so as to investigate its mechanisms underlying improvement of SCI.MethodsSD rats were randomly divided into sham operation， model and EA groups， with 15 rats in each group. The SCI model was established by using a precision impactor. EA （20 Hz/100 Hz， 1—2 mA） was applied to GV14 and GV6 for 30 min， once daily for 14 days. The rats’ hindlimb locomotor function in each group was assessed using the Basso-Beattie-Bresnahan （BBB） locomotor scale. Histopathological changes of the injured spinal cord tissue and the number of neurons were evaluated after H.E. staining and Nissl staining. The expressions of Nestin， mitochondrial fusion-related protein optic atrophy-1 （OPA1） and NSC markers sex-determining region Y-box 2 （SOX2） in the injured spinal cord tissue were detected by immunofluorescence staining. The protein and mRNA expression levels of Nestin in the spinal cord tissue were detected by quantitative real-time PCR and Western blot， separately.ResultsCompared with the sham operation group， the BBB scores after modeling， and the number of neurons were significantly decreased （P<0.001）， while the mean fluorescence intensity values of Nestin， SOX2 and OPA1， and the expressions of Nestin mRNA and protein considerably increased （P<0.001， P<0.01， P<0.05） in the model group. After EA intervention and in comparison with the model group， the BBB scores at the 7th and 14th day， the number of neurons， the mean fluorescence intensity values of Nestin， SOX2 and OPA1， and the expressions of Nestin mRNA and protein were strikingly increased （P<0.05， P<0.01， P<0.001） in the EA group. H.E. staining showed swollen， ruptured and necrotic neurons of the spinal cord， with a large number of vacuoles and severe inflammatory cell infiltration after modeling， which was relatively milder in the EA group.ConclusionEA stimulation of GV14 and GV6 can promote the recovery of motor function in rats with SCI， which may be related to its effects in promoting mitochondrial fusion and enhancing the proliferation and differentiation of NSCs.关键词：Electroacupuncture;Governor Vessel;Spinal cord injury;Mitochondrial fusion;Neural stem cells;Proliferation and differentiation69|4|0更新时间：2024-02-21
- 摘要：ObjectiveTo investigate the neuroprotective effect of electroacupuncture （EA） at “Quchi”（LI11） and “Zusanli”（ST36） in the rats with cerebral ischemia reperfusion injury and its influence on programmed necrosis of cerebral cortical neurons.MethodSixty male SD rats were randomly divided into sham-operation group， model group， EA group and inhibitor group， with 15 rats in each group. Left middle cerebral artery occlusion model was established using the modified thread embolism method. In the sham-operation group， the carotid artery was exposed and dissociated in each rat. EA was applied to “Quchi”（LI11） and “Zusanli”（ST36） on the right side for 30 min each time， once daily for 7 days in the rats of the EA group. The rats in the inhibitor group were intraperitoneally injected with norstatin-1 （0.6 mg/kg） for consecutive 7 days. The neurological deficit score of rats in each group was observed. HE staining was adopted to detect the degree of pathological damage of the cerebral cortex in the infarction area. Using TUNEL staining， the apoptosis of cortical neurons in the infarction area was determined； the contents of tumor necrosis factor α （TNF-α）， interleukin （IL）-1β and IL-6 were detected by ELISA； the mRNA and protein expression of the receptor interacting protein-1 （RIP1）， the receptor interacting protein-3 （RIP3） and the substrate mixed lineage kinase like protein （MLKL） were detected by fluorescence quantitative PCR and Western blot， respectively.ResultsIn comparison with the sham-operation group， the neurological deficit score in the model group was higher（P<0.01）； HE staining showed that there was the pathological damage in the infarction area； the neuron apoptosis rate， the contents of TNF-α， IL-1β and IL-6， and the mRNA and protein expressions of RIP1， RIP3 and MLKL increased（P<0.01） in the model group. In the EA group， the neurological deficit score was reduced（P<0.01）； HE staining showed that the pathological damage was ameliorated in the infarction area； the neuron apoptosis rate， the contents of TNF-α， IL-1β and IL-6， and the mRNA and protein expressions of RIP1， RIP3， MLKL decreased（P<0.05，P<0.01） when compared with those in the model group.ConclusionEA can attenuate cerebral ischemia reperfusion injury and display its neuroprotective effect probably through inhibiting programmed necrosis of cerebral cortical neurons in the rats.关键词：Cerebral ischemia reperfusion injury;Receptor interacting protein-1/receptor interacting protein-3/substrate mixed lineage kinase like protein pathway;Programmed necrosis;Inflammatory reaction64|6|0更新时间：2024-02-21
- 摘要：ObjectiveTo observe the effect of electroacupuncture （EA） of scalp acupoint （Dingnieqian-xiexian， MS6） on expression of retinoid-related orphan receptor γT （ROR γ t）， interleukin （IL）-17A， IL-10， transfor-ming growth factor-β1 （TGF-β1）， IL-6， IL-21， and IL-17A+ T helper cells（Th） 17 and forkhead transcription factor P3 （FOXP3）+ regulatory T cells （Treg） differentiation of ischemic cortex in ischemic stroke rats， so as to explore its molecular mechanisms underlying relief of inflammatory injury of ischemic stroke.MethodsA total of 120 male SD rats were randomly assigned to sham operation， model， EA， inhibitor， agonist and EA+agonist groups， with 15 rats in each group. The ischemic stroke model was established by occlusion of the left middle cerebral artery according to Longa’s methods. For rats of the EA group and EA+agonist group， EA （2 Hz/100 Hz， 1 mA） was applied to bilateral MS6 for 30 min， once daily for 7 days. Rats of the inhibitor group received intraperitoneal injection of solution of SR1001 （RORγt inhibitor） （2.5 mg/mL， 10 mg/kg）， once daily for 7 days. Rats of the agonist and EA+agonist groups received intraperitoneal injection of solution of SR1078 （RORγt agonist） （5 mg/mL， 5 mg/kg） before EA， once daily for 7 days. Rats of the sham operation and model groups were grabbed and fixed in the same way with the other groups. The Zea-longa’s score， modified neurological severity score （mNSS） and the neurobehavioral score were assessed before and after the intervention. At the end of experiments， the ischemic cortex tissue was collected. The 2，3，5-Triphenyltetrazolium chloride （TTC） staining was used to detect the volume of cerebral infarction. The expression of RORγt mRNA was detected by real-time quantitative PCR； the protein expression levels of RORγt， IL-17A， IL-10 and TGF-β1 were detected by Western blot； the immunoactivity of IL-6 and IL-21 were detected by immunohistochemistry； the fluorescence areas of IL-17A+Th17 and FOXP3+Treg cells were measured by immunofluorescence and their ratio was calculated in the tissue of ischemic cortex.ResultsRelevant to the sham operation group， the model group had a significant increase in the Zea-Longa’s score， mNSS score， neurobehavioral score， cerebral infarct volume， expression levels of RORγt mRNA and protein， IL-17A protein， IL-6 and IL-21 immunoactivity， IL-17A+Th17 immunofluorescence intensity， and the ratio of IL-17A+Th17/FOXP3+Treg （P<0.01）， and an obvious decrease in the expression levels of TGF-β1 and IL-10 proteins and FOXP3+Treg immunofluorescence intensity （P<0.01）. In contrast to the model group， both EA and inhibitor groups had a significant decrease in the Zea-Longa’s score， mNSS score， neurobehavioral score， cerebral infarct volume， expression levels of RORγt mRNA and protein， IL-17A protein， IL-6 and IL-21 immunoactivity， IL-17A+Th17 immunofluorescence intensity， and the ratio of IL-17A+Th17/FOXP3+Treg （P<0.01， P<0.05）， and a marked increase in the expression levels of TGF-β1 and IL-10 proteins and FOXP3+Treg immunofluorescence intensity （P<0.05， P<0.01）， while the above indicators of the agonist group were all reversed （P<0.01， P<0.05）. Comparison between the agonist and EA+agonist groups showed that the Zea-Longa’s score， mNSS score， neurobehavioral score， cerebral infarct volume， expression levels of RORγt mRNA and protein， IL-17A protein， IL-6 and IL-21 immunoactivity， IL-17A+Th17 immunofluorescence intensity， and the ratio of IL-17A+Th17/FOXP3+Treg were significantly lower （P<0.01， P<0.05）， and the expression of TGF-β1 and IL-10 proteins and FOXP3+Treg immunofluorescence intensity were obviously higher （P<0.01， P<0.05） in the EA+agonist group than in the agonist group， suggesting that EA intervention can effectively weaken the effects of RORγt agonist.ConclusionEA of scalp acupoint MS6 can effectively improve the neurological function， behavior reaction and reduce cerebral infarct volume in ischemic stroke rats， which may be associated with its functions in down-regulating the expression of RORγt and promoting the balance of IL-17A+Th17/FOXP3+Treg to alleviate inflammatory injury after ischemic stroke.关键词：Acute ischemic stroke;Electroacupuncture of scalp acupoint;Retinoid-related orphan receptor γt;T helper cells;Regulatory T cells141|16|0更新时间：2024-02-21
- 摘要：ObjectiveTo observe the effects of moxibustion at “Zusanli” （ST36） on the expression levels of tumor necrosis factor （TNF）-α， TNF receptor 1 （TNF-R1）， p38 mitogen-activated protein kinase （P38 MAPK）， and transient receptor potential vanilloid 1 （TRPV1） in the colon tissue of mice with chronic ulcerative colitis （UC）， so as to explore the underlying mechanisms of moxibustion in improving visceral hypersensitivity in chronic UC.MethodsMale C57BL/6J mice were randomly divided into normal group， normal with moxibustion （NM） group， model group， and model with moxibustion （MM） group， with 10 mice in each group. The chronic UC model was established by drinking 2.5% dextran sodium sulfate for 3 cycles. Mice in the NM and MM groups received moxibustion at ST36 for 20 min， 5 days per week with a 2-day break， for a total of 4 weeks. The disease activity index （DAI） score of each group was evaluated before and after treatment. The minimum volume threshold of abdominal wall retraction reflex （AWR） was measured to observe the intestinal sensitivity of mice. The colon length was measured. The pathological changes of colon tissue were observed by HE staining. The expression of mucin in colon goblet cells was detected by periodate Scheff staining. The intestinal fibrosis was observed by Masson staining. The number of trypsin-positive cells （i.e.， mast cell） and the expression level of TNF-α in colon tissue were detected by immunofluorescence staining. The expression levels of TNF-R1， P38 MAPK and TRPV1 in colon tissue were detected by immunohistochemistry.ResultsCompared with the normal group after treatment， the model group showed increased DAI score （P<0.001）， decreased AWR minimum volume threshold （P<0.01）， shortened colon length （P<0.001）， significant inflammatory infiltration in the colon tissue， reduced mucin secretion （P<0.01）， increased collagen fiber deposition （P<0.001）， and elevated expression levels of TNF-α， TNF-R1， P38 MAPK， and TRPV1 （P<0.001， P<0.01， P<0.05）. Compared with the model group， the MM group showed decreased DAI score （P<0.01）， increased AWR minimum volume threshold （P<0.001）， elongated colon length （P<0.001）， reduced inflammatory cell infiltration， improved integrity of mucosal glandular structure， enhanced mucin secretion （P<0.01）， decreased collagen fiber deposition （P<0.001）， decreased number of mast cells in the colon tissue （P<0.001）， and decreased expression levels of TNF-α， TNF-R1， P38 MAPK， and TRPV1 （P<0.001， P<0.01， P<0.05）. There were no significant differences in the above index between the NM group and the normal group.ConclusionMoxibustion can reduce visceral hypersensitivity， alleviate inflammatory infiltration and fibrotic damage in the colon tissue of mice with chronic UC. These effects may be associated with the down-regulation of TNF-α， TNF-R1， P38 MAPK， and TRPV1 expression in colon.关键词：Moxibustion;Zusanli （ST36）;Chronic ulcerative colitis;Visceral hypersensitivity;TNF-α/TNF-R1/P38 MAPK/TRPV1 pathway64|4|1更新时间：2024-02-21
- 摘要：ObjectiveTo investigate the mechanism of electroacupuncture （EA） at “Neiguan” （PC6） in impro-ving myocardial electrical remodeling in rats with acute myocardial infarction （AMI） by enhancing transient outward potassium current.MethodsA total of 30 male SD rats were randomly divided into control， model and EA groups， with 10 rats in each group. The AMI model was established by subcutaneous injection with isoprenaline （ISO， 85 mg/kg）. EA was applied to left PC6 for 20 min， once daily for 5 days. Electrocardiogram （ECG） was recorded after treatment. TTC staining was used to observe myocardial necrosis. HE staining was used to observe the pathological morphology of myocardial tissue and measure the cross-sectional area of myocardium. Potassium ion-related genes in myocardial tissue were detected by RNA sequencing. The mRNA and protein expressions of Kchip2 and Kv4.2 in myocardial tissue were detected by real-time fluorescence quantitative PCR and Western blot， respectively.ResultsCompared with the control group， cardiomyocyte cross-sectional area in the model group was significantly increased （P<0.01）， the ST segment was significantly elevated （P<0.01）， and QT， QTc， QTd and QTcd were all significantly increased （P<0.05， P<0.01）. After EA treatment， cardiomyocyte cross-sectional area was significantly decreased （P<0.01）， the ST segment was significantly reduced （P<0.01）， and the QT， QTc， QTcd and QTd were significantly decreased （P<0.01， P<0.05）. RNA sequencing results showed that a total of 20 potassium ion-related genes co-expressed by the 3 groups were identified. Among them， Kchip2 expression was up-regulated most notablely in the EA group. Compared with the control group， the mRNA and protein expressions of Kchip2 and Kv4.2 in the myocardial tissue of the model group were significantly decreased （P<0.01， P<0.05）， while those were increased in the EA group （P<0.01， P<0.05）.ConclusionEA may improve myocardial electrical remodeling in rats with myocardial infarction， which may be related to its functions in up-regulating the expressions of Kchip2 and Kv4.2.关键词：Electroacupuncture;Myocardial infarction;Myocardial electrical remodeling;Transient outward potassium current54|4|0更新时间：2024-02-21
- 摘要：ObjectiveTo observe the effects of the local stimulation with 3 acupuncture techniques， i.e. Canggui Tanxue （needle insertion method like dark tortoise detecting point） technique， electroacupuncture （EA） and warm needling （WN） with filiform needles on shoulder pain， shoulder joint function， quality of life， inflammatory indicators and recurrence rate in the patients with chronic scapulohumeral periarthritis （CSP）， so as to explore the optimal needling method of acupuncture for the predominant symptoms of CSP during the attack stage in the patients.MethodsA total of 108 patients with CSP were randomly divided into a manual acupuncture （MA） group （36 cases， one case dropped off）， a WN group （36 cases， 3 cases dropped off） and an EA group （36 cases， 1 case dropped off）. In the three groups， Jianqian （EX-UE12）， Jianyu （LI15）， Jianzhen （SI9）， Ashi （Extra） and Yanglingquan （GB34） on the affected side were selected. Canggui Tanxue needling technique， WN technique and EA were delivered in the MA group， the WN group and the EA group， respectively， 30 min each time， 3 times weekly for 4 weeks. The Neer test scores were compared； the visual analogue scale （VAS） was used to assess the degree of shoulder joint pain； the daily life activity abilities was evaluated using the activities of daily living （ADL） scale； the serum prostaglandin E2 （PGE2） content was measured using ELISA before and after treatment. The effectiveness rate and recurrence rate were calculated， and the occurrences of adverse reactions were recorded.ResultsCompared with the scores before treatment， the scores of pain， joint function， and range of motion as well as the total score of Neer test were all increased after treatment in the three groups （P<0.05）； the VAS score， ADL score and the content of serum PGE2 were decreased （P<0.05）. After treatment， the pain score of Neer test in the EA group and the WN group were higher than those of the MA group （P<0.05）， the joint function score of Neer test in the MA group and the WN group were higher than that of the EA group （P<0.05）， and the range of motion score of Neer test in the MA group was higher when compared with the EA and WN groups （P<0.05）. There was no statistical difference in the total score of Neer score among the three groups. VAS score in the EA group was lower than that of either the WN group or the MA group （P<0.05）. ADL score in the MA group was lower compared with that of the WN group （P<0.05）. PGE2 levels in both the WN group and the MA group were lower than that of the EA group （P<0.05）. The total effective rate was 85.71% （30/35） in the MA group， 91.43% （32/35） in the EA group and 90.91% （30/33） in the WN group， there was no statistical differences among the three groups. At the end of the 6-month follow-up visit after treatment， there was no significant difference in the recurrence rate among three groups. No serious adverse reaction was found.ConclusionIn the treatment of CSP， the short-term effect is equivalent among EA， WN and MA. But， the analgesic effect is the best in the EA group， the treatment for anti-inflammation is the most effective in the MA and WN groups， and the needling technique of Canggui Tanxue in the MA group obtains the most favorable effect of releasing adhesion and recovering the range of motion in the shoulder joint.关键词：Chronic scapulohumeral periarthritis;Manual acupuncture;Warm needling;Electroacupuncture;Comparison of different techniques of acupuncture240|22|0更新时间：2024-02-21
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Effect of moxibustion for “nourishing the kidney and benefiting the marrow” on knee osteoarthritis AI导读
摘要：ObjectiveTo compare the clinical effect between two acupoint regimens of moxibustion on knee osteoarthritis （KOA）， and observe the influences on the serum content of interleukin 1α （IL-1α）， interleukin-17A （IL-17A）， tumor necrosis factor α （TNF-α）， bone gla protein （BGP） and osteoprotegerin （OPG）.MethodsKOA patients were randomly divided into an observation group （40 cases， 2 cases dropped off） and a control group （40 cases， 3 cases dropped off）. In the observation group， moxibustion was applied to Xiyan （EX-LE5）， Dubi （ST35）， Zusanli （ST36）， Dazhu （BL11）， Xuanzhong （GB39） and Yongquan （KI1） on the affected side. In the control group， EX-LE5， ST35 and ST36 were selected on the affected side. One session of treatment took 30 min in each group， delivered 3 times a week and the duration of treatment was 4 weeks. The scores of Western Ontario and McMaster University （WOMAC） and visual analogue scale （VAS） were observed and the serum content of IL-1α， IL-17A， TNF-α， BGP and OPG of the two groups were measured before and after treatment.ResultsCompared with those before treatment， the WOMAC score， VAS score and the serum content of IL-1α， IL-17A and TNF-α were decreased （P<0.05）， and the content of BGP and OPG were increased （P<0.05） after treatment. Compared with the control group， the WOMAC score， VAS score and the serum content of IL-1α and TNF-α in the observation group were lower （P<0.05）， and the content of BGP and OPG were higher （P<0.05）. The total effective rate of the observation group was 89.5% （34/38）， and that of the control group was 83.8% （31/37）， with no statistically significant difference.ConclusionMoxibustion therapy of “nourishing the kidney and benefiting the marrow” can relieve joint pain， improve joint function， reduce the level of inflammatory factors and ameliorate bone metabolic indicators. The effect of the acupoint regimen in this moxibustion therapy is better than that of the local acupoint selection.关键词：Moxibustion;Knee osteoarthritis;Bone metabolism;Inflammatory factors89|4|0更新时间：2024-02-21 - 摘要：ObjectiveTo observe the curative efficacy of auricular comprehensive therapy on menstrual migraine（MM） and its effect on serum prostaglandin F2α（PGF2α）， prostaglandin E2（PGE2） contents and ratio， so as to explore its possible mechanism.MethodsA total of 66 patients with MM of liver-fire syndrome were randomly divided into observation group （33 cases， 2 cases dropped off） and control group （33 cases， 2 cases dropped off）， and 20 healthy women were included in the normal group. Patients in the control group were given flunarizine hydrochloride capsules orally， twice a day， for 3 consecutive weeks. Patients in the observation group were treated with auricular comprehensive therapy， starting 2—5 days before menstrual cramps， once a week， for a total of 3 weeks. The visual analogue scale （VAS） and migraine score were evaluated before and after treatment， and follow-up for 1 and 2 menstrual cycles. Serum PGF2α and PGE2 contents were measured before and after treatment， and the PGF2α/PGE2 ratio was calculated. The clinical effective rates in the two groups were calculated.ResultsAfter treatment and follow-up for 1 and 2 menstrual cycles， the VAS scores， headache degree， the frequency and duration of headache attacks， as well as accompanying symptoms of the observation and control groups were lower than those before treatment（P<0.05）， and those of the observation group was lower than those of the control group（P<0.05）. Before treatment， the PGF2α contents in the observation and control group were significantly higher（P<0.05）， while the PGE2 contents lower（P<0.05） and PGF2α/PGE2 ratio higher（P<0.05） than those in the normal group. After treatment， the serum PGF2α contents in the observation and control group were significantly reduced compared with which before treatment（P<0.05）， and were lower in the observation group than that in the control group （P<0.05）. The serum PGE2 contents in the observation and control groups were significantly increased after treatment compared with which before treatment（P<0.05）， with the contents in the observation group higher than that in the control group（P<0.05）. The serum PGF2α/PGE2 ratio in the observation and control group was significantly reduced after treatment compared with which before treatment（P<0.05）， with the control group higher than the normal group（P<0.05）， and the observation group lower than the control group（P<0.05）. The clinical effective rate of the observation group was 93.5% （29/31）， and that of the control group was 77.4% （24/31）. The effective rate of the observation group was significantly higher than that of the control group（P<0.05）.ConclusionThe curative efficacy of auricular comprehensive therapy on MM with liver-fire syndrome is significantly better than that of oral flunarizine hydrochloride capsules， especially in relieving hea-daches， reducing the frequency and duration of headache attacks， as well as accompanying symptoms. Its mechanism may be related to regulating the abnormal PGF2α and PGE2 contents of patients and reducing the ratio of PGF2α/PGE2.关键词：Auricular point comprehensive therapy;Menstrual migraine;Prostaglandin F2α;Prostaglandin E296|11|0更新时间：2024-02-21
- 摘要：The article summarizes the relevant factors to the therapeutic effect of moxibustion on knee osteoarthritis， including the origin and storage time of moxa leaves， the time of moxibustion， the numbers of moxa cone， and the temperature when moxibustion is operated. Artemisia mugwort in Qichun county stored for over 3 years is the best regarding its property； and it is recommended for about 40 min in suspended moxibustion； and the heat-sensitive moxibustion is determined when the sensation of moxibustion disappears； and in terms of moxibustion techniques and the numbers of moxa cone， two moxa cones are optimal in warm needling， but the highly applicable duration of moxibustion needs to be confirmed through more high-quality studies. There are few studies on the other influencing factors， such as the specific operation of suspended moxibustion， the angle of knee flexion， treatment sequence， light and smoking factors， moxibustion method and disease staging and type； and the studies are limited in the comparison in terms of the middle-term and long-term efficacy， the comparison of the efficacy among different syndromes of traditional Chinese medicine in patients and the comparison among various frequencies and sessions of treatment. In future， more high-quality clinical trials should be designed to complete the evidence-based regimens and optimize clinical operations.关键词：Moxibustion;Knee osteoarthritis;Influencing factors74|18|1更新时间：2024-02-21
- 摘要：Neuroimaging technology provides objective and visualized research tool to study the mechanisms of acupuncture effects. Building on a systematic review of previous clinical studies on acupuncture treatment for functional dyspepsia using neuroimaging technology， this paper summarizes and synthesizes past researches from 4 aspects： acupoint-specific effects， factors influencing the effects， different physiological responses， and predictive factors for acupuncture efficacy. It suggests that acupuncture treatment for FD involves central integration with disease-targeted （acupuncture treatment can target and regulate abnormal brain functional activity patterns in patients with FD）， meridian-specific （stimulation of specific acupuncture points along the stomach meridian can significantly regulate abnormal brain functional activity patterns in FD patients）， and dynamic conditional features（the effects of acupuncture treatment for FD are influenced by multiple factors）. Lastly， considering the current research status， this paper outlines prospects in terms of research subjects， influencing factors， and result validation， aiming to provide references for future in-depth research.关键词：Functional dyspepsia;Acupuncture;Neuroimaging;Functional magnetic resonance imaging85|18|0更新时间：2024-02-21
- 摘要：ObjectiveTo explore the rules of acupoint selection in the treatment of neurogenic bladder （NB） with acupuncture and moxibustion by using data mining.MethodsThe clinical research literatures on acupuncture treatment of NB were collected from PubMed， Embase， Cochrane Library， CNKI， Wanfang Database， VIP Database and China Biology Medicine from retrieved to January 1， 2023. The acupoint prescription database was established using Excel 2019. SPSS Modeler 18.0 and SPSS Statistics 26.0 softwares were used to conduct the frequency， meri-dians， locations， specific acupoints analysis and association rules analysis， factor analysis， cluster analysis， etc.， to explore the characteristics and rules of acupoint selection in acupuncture and moxibustion treatment of NB.ResultsTotally 313 papers were included， including 110 acupoints with a total frequency of 1 995. The high-frequency acupoints are Zhongji （CV3）， Guanyuan （CV4）， Sanyinjiao （SP6）， etc. The commonly used meridians are the Bladder Meridian of Foot Taiyang and Conception Vessel. The involved acupoints are mostly located in the lumbosacral region and abdomen， and intersection acupoints， mu-front acupoints and back-shu acupoints are the majority in the specific acupoints. The core acupoints group was analyzed， and 17 groups of association rules， 7 factors and 6 effective cluster groups were obtained.ConclusionAcupuncture and moxibustion treatment of NB follows the therapeutic principles of toni-fying the kidney， invigorating the spleen， and soothing the liver. The core acupoints group is CV3-CV4-SP6.关键词：Acupuncture and moxibustion;Neurogenic bladder;Acupoint selection rules;Data mining75|7|0更新时间：2024-02-21
- 摘要：ObjectiveTo evaluate the efficacy of acupuncture in the treatment of postoperative gastrointestinal dysfunction（POGD） of colorectal cancer.MethodsRandomized controlled trials of acupuncture in the treatment of POGD were retrieved from 7 databases including PubMed， Embase， Cochrane Library， China National Knowledge Infrastructure， VIP Chinese Journal Service Platform， WanFang Data Knowledge Service Platform， and China Biology Medicine disc. The search period ranged from the inception of the databases to November 10th， 2022. The quality of the included literature was assessed using the Cochrane bias risk assessment tool and the modified Jadad scale. Meta analysis was conducted using RevMan 5.4. Regression analysis and bias risk analysis were performed using Stata 16.0. Trial sequential analysis was conducted using TSA 0.9 software.ResultsA total of 27 randomized controlled trials involving 2 629 patients were included. Intervention measures included manual acupuncture， electroacupuncture， transcutaneous acupoint electrical stimulation， warm acupuncture， and thumb-tack needle. The results showed that acupuncture treatment significantly reduced time to tolerance of liquid diet after surgery （MD=-13.70，95% CI=［-17.94，-9.46］，P<0.000 01）， time to first defecation （MD=-18.20， 95% CI=［-22.62，-13.78］， P<0.000 01）， time to first flatus （MD=-16.31，95% CI=［-20.32， -12.31］，P<0.000 01）， time to bowel sounds recovery （MD=-11.91，95% CI=［-14.01，-9.81］，P<0.000 01）， and length of hospital stay （MD=-1.49，95% CI=［-2.27，-0.70］，P=0.000 2）. Regression analysis indicated that cancer type， study quality and number of acupuncture were the main sources of heterogeneity. Bias analysis suggested potential publication bias risks. Trial sequential analysis indicated that the required number of cases had been met and the conclusion was reliable.ConclusionAcupuncture is an effective intervention for promoting gastrointestinal recovery in patients undergoing colorectal cancer surgery. Further large-sample and well-designed clinical trials are still needed to compare different acupuncture techniques.关键词：Acupuncture;Colorectal cancer;Postoperative gastrointestinal dysfunction;Meta analysis206|14|0更新时间：2024-02-21
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