最新刊期
卷 49 ， 期 3 ， 2024
- “电针治疗帕金森病（PD）的机制研究取得了新进展。专家通过对比电针组和假电针组小鼠的运动能力、中脑黑质区相关蛋白表达水平等指标，发现电针“风府”“太冲”“足三里”能显著改善PD小鼠的运动能力，其机制可能是通过激活SIRT3/PINK1/Parkin通路，增强线粒体自噬，促进中脑黑质酪氨酸羟化酶（TH）表达，减少α-突触核蛋白（α-syn）聚集，从而发挥神经保护作用。这一发现为PD治疗提供了新的思路和方法。”摘要：ObjectiveTo observe the effects of electroacupuncture （EA） at “Fengfu”（GV16）， “Taichong”（LR3）， and “Zusanli”（ST36） on mitophagy mediated by silencing regulatory protein 3 （SIRT3）/ PTEN induced putative kinase 1 （PINK1）/PARK2 gene coding protein （Parkin） in the midbrain substantia nigra of Parkinson’s disease （PD） mice， and to explore the potential mechanisms of EA in treating PD.MethodsC57BL/6 mice were randomly divided into the control， model， EA， and sham EA groups， with 12 mice in each group. The PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine （MPTP）. The EA group received EA stimulation at GV16， LR3 and ST36， while the sham EA group received shallow needling 1 mm away from the above acupoints without electrical stimulation. The motor ability of mice in each group was evaluated using an open field experiment. Immunohistochemistry was used to detect the expression of tyrosine hydroxylase （TH） and α-synuclein （α-syn） in the substantia nigra of mice. The ultrastructure of neurons in substantia nigra was observed by transmission electron microscope （TEM）. Immunofluorescence was used to detect the expression of the autophagy marker autophagy-associated protein light chain 3 （LC3）. The expression levels of TH， α-syn， SIRT3， PINK1， Parkin， P62， Beclin-1， LC3Ⅱ mRNA and protein were detected by PCR and Western blot.ResultsCompared with the control group， mice in the model group showed a decrease in the total exercise distance， time， movement speed and times of crossing central region （P<0.01）； the positive expressions of TH and LC3 were decreased （P<0.01）， while the positive expression of α-syn increased （P<0.01）， accompanied by mitochondrial swelling， mitochondrial cristae fragmentation and decrease， and decreased lysosome count； the expression levels of TH， SIRT3， PINK1， Parkin， Beclin-1， and LC3Ⅱ mRNA and protein in the midbrain substantia nigra were decreased （P<0.01）， while the expression levels of α-syn and P62 mRNA and protein were increased （P<0.01， P<0.05）. Compared with the model group， the mice in EA group showed a significant increase in the total exercise distance， time， movement speed and times of crossing central region （P<0.01， P<0.05）； the positive expressions of TH and LC3 were increased （P<0.01， P<0.05）， while the positive expression of α-syn was decreased （P<0.01）， accompanied by an increase in mitochondrial count， appearance of autophagic va-cuoles， and a decrease in swelling， the expression levels of TH， SIRT3， PINK1， Parkin， Beclin-1 and LC3Ⅱ mRNA and protein in the midbrain substantia nigra were increased （P<0.01， P<0.05）， while the mRNA and protein expression levels of α-syn and P62 were decreased （P<0.01）； the sham EA group showed an increase in the total exercise distance and time（P<0.05）， with an increase in the positive expression of TH （P<0.05） and a decrease in the positive expression of α-syn （P<0.05）； some mitochondria exhibited swelling， and no autophagic vacuoles were observed； the protein expression levels of TH， SIRT3， Parkin and LC3Ⅱ were increased （P<0.01， P<0.05）， and the expression levels of P62 mRNA， α-syn mRNA and protein were decreased （P<0.01， P<0.05）， and LC3Ⅱ mRNA expression was increased （P<0.05）. In comparison to the sham EA group， the EA group showed an extension in the total exercise time （P<0.01）， the positive expression and mRNA expression levels of α-syn were decreased （P<0.01， P<0.05）， while the expression levels of TH， SIRT3， PINK1， Parkin mRNA and SIRT3 protein were increased （P<0.05）.ConclusionEA at GV16， LR3， and ST36 can exert neuroprotective function and improve the motor ability of PD mice by activating the SIRT3/PINK1/Parkin pathway to enhance the expression of TH and reduce α-syn aggregation in the substantia nigra of PD mice.关键词：Parkinson’s disease;Electroacupuncture;silencing regulatory protein 3;PTEN induced putative kinase 1;PARK2 gene coding protein;Mitophagy196|15|0更新时间：2024-05-09
- “一项关于艾灸联合贝那普利治疗慢性心力衰竭（CHF）的研究取得了重要进展。该研究通过复制CHF大鼠模型，观察了艾灸“心俞”“肺俞”联合贝那普利对心功能及心肌组织中关键蛋白的影响。结果显示，艾灸联合药物治疗能有效改善大鼠的心功能，降低血清中的B型脑钠肽（BNP）和血管紧张素Ⅱ（AngⅡ）含量，同时减少心肌组织中的磷酸化PERK和eIF2α蛋白表达。这一发现为艾灸联合药物治疗CHF提供了科学依据，为未来的临床研究和应用提供了新的思路。”摘要：ObjectiveTo observe the effect of moxibustion at “Xinshu”（BL15） and “Feishu”（BL13） combined with intraperitoneal injection of benazepril on cardiac function and phosphorylation of protein kinase R-like endoplasmic reticulum kinase （PERK） and eukaryotic initiation factor 2α （elF2α） proteins in myocardium of rats with chronic heart failure （CHF）， so as to explore its potential mechanism underlying improvement of CHF.MethodsA total of 42 male SD rats were randomly assigned to blank control （n=10）， CHF model （n=7）， medication （benazepril， n=8）， moxibustion （n=8） and moxibustion+benazepril （n=9） groups， after cardiac ultrasound model identification and elimination of the dead. The CHF model was established by intraperitoneal injection of doxorubicin hydrochloride （DOX）， once every week for 6 weeks. Mild moxibustion was applied to bilateral BL15 and BL13 regions for 20 min， once daily for 3 weeks. The rats of the medication group and moxibustion+benazepril group （benazepril was given first， followed by moxibustion） received intraperitoneal injection of benazepril （0.86 mg/kg） solution once daily for 3 weeks . The cardiac ejection fraction （EF） and left ventricular fractional shortening （FS） were measured using echocardiography. Histopathological changes of the cardiac muscle tissue were observed under light microscope after hematoxylin-eosin （H.E.） staining. Serum contents of B-type brain natriuretic peptide （BNP） and angiotensin Ⅱ （AngⅡ） were measured by enzyme-linked immunosorbent assay （ELISA）. The expressions of phospho-PERK （p-PERK） and phospho-elF2α （p-elF2α） in the myocardium were detected by Western blot.ResultsCompared with the blank control group， the EF and FS of the left cardiac ventricle were significantly decreased （P<0.01）， while the contents of serum BNP and AngⅡ， and expression levels of p-PERK and p-eIF2α significantly increased in the model group （P<0.01）. In comparison with the model group， both the decreased EF and FS and the increased BNP and AngⅡ contents as well as p-PERK and p-elF2α expression levels were reversed by moxibustion， medication and moxibustion+benazepril （P<0.01）. The effects of moxibustion+benazepril were markedly superior to those of simple moxibustion and simple medication in raising the levels of EF and FS rate and in down-regulating the contents of BNP， Ang Ⅱ， levels of p-PERK and p-elF2α （P<0.01， P<0.05）. Outcomes of H.E. staining showed irregular arrangement of cardiomyocytes， cell swelling， vacuole and inflammatory infiltration in the model group， which was relatively milder in the 3 treatment groups. The effects of moxibustion+benazepril were superior to those of moxibustion or benazepril.ConclusionMoxibustion combined with Benazepril can improve the cardiac function in CHF rats， which may be related to its functions in down-regulating the expression levels of myocardial p-PERK and p-elF2α to inhibit endoplasmic reticulum stress response.关键词：Moxibustion;Chronic heart failure;Cardiac function;Endoplasmic reticulum stress79|3|0更新时间：2024-05-09
- “有关艾灸预处理对脑缺血再灌注损伤大鼠炎性因子影响的研究取得新进展。实验显示，通过激活PI3K/AKT/mTOR信号通路，艾灸预处理能显著改善CIRI大鼠的神经功能，抑制血清炎性因子IL-1β、TNF-α，并抑制脑组织损伤标志物S-100β蛋白和NSE。其中，预处理7天的效果最为显著，优于5天和3天。这一发现不仅为脑缺血再灌注损伤的治疗提供了新的思路，也为艾灸在神经保护领域的应用提供了实验支持。”摘要：ObjectiveTo observe the effect of moxibustion preconditioning on inflammatory response in rats with cerebral ischemia reperfusion injury （CIRI）， so as to explore its mechanisms underlying improving CIRI.MethodsSeventy-five male SD rats were randomly divided into sham operation， model， moxibustion preconditioning 3 days （Moxi 1）， moxibustion preconditioning 5 days （Moxi 2） and moxibustion preconditioning 7 days （Moxi 3） groups， with 15 rats in each group. Moxibustion was applied at “Baihui”（GV20），“Dazhui”（GV14） and “Zusanli”（ST36） for 20 min once a day， totally for 3， 5 or 7 days. Thirty minutes after the last moxibustion treatment， the CIRI model was established by occlusion of the middle cerebral artery. The neurological deficit score was assessed by using Longa’s method. The infarct size of the brain assessed after staining with 2% triphenyltetrazolium chloride （TTC）. The morphological changes of cortical neurons were observed by HE staining. The contents of inflammatory factors interleukin-1β （IL-1β）， tumor necrosis factor-α （TNF-α）， S-100β protein （S-100β） and neuron-specific enolase （NSE） were detected by ELISA. The expression of phosphatidylinositol-3-kinase （PI3K）， p-PI3K， protein kinase B （AKT） and mammalian target of rapamycin （mTOR） proteins in the ischemic cortex tissues were detected by immunohistochemistry and Western blot.ResultsCompared with the sham operation group， the neurological function score and the percentage of cerebral ischemic volume were increased （P<0.01）. The contents of serum IL-1β， TNF-α， S-100β and NSE were significantly increased （P<0.01）， while the protein expressions of PI3K， p-PI3K， AKT and mTOR in the cerebral cortex were significantly decreased （P<0.01） in the model group. Compared with the model group， the neurological function score and the percentage of cerebral ischemic volume were significantly decreased （P<0.01）. The contents of serum IL-1β， TNF-α， S-100β and NSE were significantly decreased （P<0.01）， and the expressions of PI3K， p-PI3K， AKT and mTOR proteins in the cerebral cortex were significantly increased （P<0.01） in three moxibustion groups. Compared with the Moxi 1 and Moxi 2 groups， the above indicators were significantly improved in rats of the Moxi 3 group （P<0.01， P<0.05）.ConclusionMoxibustion preconditioning can significantly improve the neurological function of rats after ischemia-reperfusion， inhibit serum inflammatory factors IL-1 β and TNF-α， inhibit brain tissue injury markers S-100β and NSE， which may be related to the activation of PI3K/AKT/mTOR signaling pathway. The protective effect of moxibustion preconditioning for 7 days on CIRI was better than that of 5 days and 3 days.关键词：Moxibustion preconditioning;Cerebral ischemia reperfusion injury;PI3K/AKT/mTOR signaling pathway73|4|0更新时间：2024-05-09
- “刮痧对膝关节骨关节炎大鼠的关节软骨损伤有显著改善作用。研究发现，刮痧能抑制炎性因子的过量释放，减少软骨细胞凋亡率。这归功于刮痧上调了Beclin-1和LC3的表达，同时降低了P62和Caspase-3的表达，促进了软骨细胞的自噬过程。这项研究为刮痧治疗膝关节骨关节炎提供了新的科学依据，为关节炎治疗领域带来了新的启示。”摘要：ObjectiveTo observe the effect of Guasha on inflammation factors， apoptosis and autophagy in the cartilage tissue of knee joint in rats with knee osteoarthritis （KOA）， so as to explore its mechanisms underlying improvement of KOA.MethodsA total of 51 male SD rats were randomized into three groups： blank control， KOA model and Guasha （n= 17 in each group） . The rats in the blank control group received intra-articular injection of 0.9% NaCl solution in the right knee joint. The KOA model was established by intraarticular injection of glutamate sodium iodoacetic acid in the right knee joint. For rats of the Guasha group， Guasha （at a frequency of 1 time/s， and an applied pressure of 0.3—0.5 kgf） was applied to “Yanglingquan” （GB34） and “Xuehai”（SP10） areas of the right leg， once every other day， for 7 consecutive sessions. The circumference of the right knee was measured， The histopathological changes of right knee cartilage were observed after H.E. staining. The contents of inflammatory factors interleukin （IL）-1β and tumor necrosis factor （TNF）-α in the right knee articular cartilage tissue were assayed using ELISA. The expression levels of autophagy-related key molecule Beclin-1 （homologous series of yeast Atg6）， light chain protease complication 3 type II/I （LC3II/LC3 I）， ubiquitin binding factor 62 （P62） and cysteine aspartate protease-3 （Caspase-3） mRNAs and proteins of the right knee articular cartilage tissue were measured using real-time fluorescent quantitative PCR and Western blot， separately. The apoptosis of chondrocytes was assayed using TUNEL staining， and the immunoactivity of LC3 determined using immunofluorescence staining.ResultsAfter modeling， the right knee circumfe-rence of the model and Guasha groups was significantly increased compared with the blank control group （P<0.01）， and after the intervention， the knee circumference of the Guasha group was markedly decreased in comparison with that of the model group （P<0.05）. Results of H.E. staining showed obvious degeneration and defects in the cartilage tissue， necrosis of a large number of chondrocytes， fibrous hyperplasia， accompanied by inflammatory cell infiltration， osteoclast increase， fibroplasia and bone trabecular destruction in the model group， which was relatively milder in the Guasha group. Compared with the blank control group， the expression of Beclin-1 and LC3 mRNAs and proteins， and LC immunofluorescence intensity in the right knee articular cartilage tissue were significantly down-regulated （P<0.01，P<0.001）， whereas the expression of P62 and Caspase-3 mRNAs and proteins， the apoptosis rate， contents of IL-1β and TNF-α in the right knee articular cartilage tissue considerably increased （P<0.01，P<0.001） in the model group. In contrast to the model group， the Guasha group had an apparent increase in the expression levels of Beclin-1 and LC3 mRNAs and proteins and LC immunofluorescence intensity in the right knee articular cartilage tissue （P<0.05）， and a pronounced decrease in the expression of P62 and Caspase-3 mRNAs and proteins， the apoptosis rate， and contents of IL-1β and TNF-α in the right knee articular cartilage tissue （P<0.05，P<0.01）.ConclusionGuasha stimulation of GB34 and SP10 can improve joint cartilage damage in KOA rats， which may be associated with its functions in inhibiting the excessive release of inflammatory factors and apoptosis， possibly by down-regulating the expression of P62 and Caspase-3 mRNAs and proteins and up-regulating the expression of Beclin-1 and LC3 mRNAs and proteins， and by promoting autophagy of chondrocytes.关键词：Guasha;Knee osteoarthritis;Autophagy;Apoptosis121|9|0更新时间：2024-05-09
- “有研究者深入探讨了电针治疗帕金森病的作用机制，为神经退行性疾病的治疗提供了新视角。该研究通过对比帕金森病模型小鼠与电针干预后的小鼠，发现电针可显著改善模型小鼠的运动障碍、步态紊乱及肠道运动功能，并保护多巴胺能神经元免受氧化应激损伤。此研究不仅证实了电针在神经退行性疾病中的潜在应用价值，也为后续研究提供了新的方向。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on behavior， oxidative stress factors in colon and substantia nigra of Parkinson’s disease （PD） mice， so as to explore the mechanism of EA in treating PD.MethodsC57BL/6 mice were randomly divided into blank， model and EA groups， with 12 mice in each group. The PD mouse model was established by continuous gavage of rotenone for 4 weeks. Mice in the EA group received EA （2 Hz/15 Hz） at “Baihui” （GV20）， “Quchi” （LI11） and “Zusanli” （ST36） for 20 min， 5 days a week for 2 weeks. After intervention， gait analysis was used to evaluate the motor ability and motor coordination. Ink propulsion rate was used to evaluate the intestinal transport function. The level of reactive oxygen species （ROS） in the colon was detected by flow cytometry. The contents of total protein （TP）， malondialdehyde （MDA） and activities of glutathione peroxidase （GSH-Px）， superoxide dismutase （SOD） in colon and substantia nigra were detected by ELISA. The expression of nuclear factor E2-related factor 2 （Nrf2） in substantia nigra was detected by immunofluorescence staining.ResultsCompared with the blank group， the average speed， step rate， normal step ratio， distance between the front and hind feet， stride length， swing speed and maximum intensity of the maximum contact area of mice in the model group were decreased （P<0.000 1， P<0.01， P<0.001）， the maximum change rate of gait was increased （P<0.001） in the model group. The intestinal propulsion rate， the activities of GSH-Px and SOD in the colon and substantia nigra， and the positive expression of Nrf2 in substantia nigra were decreased （P<0.000 1， P<0.01， P<0.05）， while the fluorescence intensity of ROS in the colon， the contents of MDA in colon and substantia nigra were increased （P<0.01）. Compared with the model group， the average speed， step rate， normal step ratio， distance between the front and hind feet， stride length， swing speed， and maximum intensity of the maximum contact area of the mice in the EA group were increased （P<0.01， P<0.05， P<0.001， P<0.000 1）， the maximum change rate of gait was decreased （P<0.01）. The intestinal propulsion rate， the activities of GSH-Px and SOD in the colon and substantia nigra， the positive expression of Nrf2 in substantia nigra were increased （P<0.001， P<0.05， P<0.000 1）， while the ROS fluorescence intensity in the colon， the MDA contents in the colon and substantia nigra were decreased （P<0.01）.ConclusionEA can improve the movement disorder， gait disorder and intestinal motor function of PD mice， and protect dopaminergic neurons from damage， which may be related to its effect in antagonistic brain-gut oxidative stress.关键词：Parkinson’s disease;Electroacupuncture;Oxidative stress;Brain-gut axis;Dopaminergic neurons73|5|0更新时间：2024-05-09
- “本研究观察了电针对糖尿病认知功能障碍（DCI）大鼠海马组织内信号通路及相关蛋白的影响，探讨了电针治疗DCI的机制。实验结果显示，电针能改善DCI大鼠的学习记忆能力，这可能与调控PI3K/Akt/CREB信号通路相关蛋白表达、减轻海马神经元凋亡有关。这项研究为电针治疗DCI提供了理论基础，为糖尿病神经并发症的治疗开辟了新方向。”摘要：ObjectiveTo observe the effects of electroacupuncture （EA） on the phosphatidylinositol-3-kinase （PI3K）/protein kinase B （Akt）/cAMP response element binding protein （CREB） signaling pathway-related proteins and hippocampal neuron apoptosis in diabetic cognitive impairment （DCI） rats， and to explore the mechanisms of EA in treating DCI.MethodsAdult male SD rats were randomly divided into normal， model， and EA groups， with 12 rats in each group. The animal model of DCI was replicated using a high-fat， high-sugar diet combined with low-dose streptozotocin. The EA group received EA stimulation at “Yishu” （EX-B6）， “Zusanli” （ST36）， “Baihui” （GV20）， and “Dazhui” （GV14）. Blood glucose contents of the rats in each group were measured. The Morris water maze test was used to assess the learning and memory abilities of rats. Transmission electron microscopy was used to observe the ultrastructure of hippocampal CA1 neurons. Nissl staining was used to observe the pathological changes in hippocampal CA1 neurons. TUNEL staining was used to detect the apoptosis in hippocampal CA1 neurons. Western blot was used to detect the protein expression levels of p-PI3K/PI3K and p-Akt/Akt， as well as CREB， p-CREB， cysteine aspartate pro-tease （Caspase）-3， B-cell lymphoma-2 （Bcl-2）， and Bcl-2 related X protein （Bax） in the hippocampal tissue of rats.ResultsCompared with the normal group， the rats’ random blood glucose contents were significantly increased （P<0.01）， the escape latency prolonged （P<0.01）， and the original platform crossing counts reduced （P<0.01） in the model group. Significant damage to hippocampal CA1 neurons， a significantly increased neuronal apoptosis index （P<0.01）， decreased ratio of p-PI3K/PI3K and p-Akt/Akt and expression of CREB， p-CREB and Bcl-2 proteins， increased expression of Caspase-3 and Bax proteins （P<0.01） were observed in the hippocampal tissue of rats in the model group. Compared with the model group， the rats in the EA group showed decreased random blood glucose content （P<0.01）， shortened escape latency （P<0.01）， increased original platform crossing counts （P<0.01）， improved quantity and pathological morphology and ultrastructure of hippocampal CA1 neurons， reduced neuronal apoptosis index （P<0.01）， increased ratio of p-PI3K/PI3K and p-Akt/Akt， and expression of CREB， p-CREB and Bcl-2 proteins （P<0.05， P<0.01） in the hippocampal tissue， and decreased expression of Caspase-3 and Bax proteins （P<0.01）.ConclusionEA can improve the learning and memory abilities of rats with DCI， and the mechanism may be related to the regulation of the expression of PI3K/Akt/CREB signaling pathway-related proteins， which attenuates the neuronal apoptosis in the hippocampus of rats， and improves the neural function.关键词：Diabetic cognitive impairment;Electroacupuncture;Apoptosis;PI3K/Akt/CREB signaling pathway76|7|0更新时间：2024-05-09
- “电针治疗对慢性疲劳综合征大鼠的行为学和海马炎性因子有显著影响。研究通过改良慢性多因素复合应激刺激法建立CFS模型，并用电针干预。结果显示，电针能降低大鼠疲劳程度，提高学习记忆能力，同时改善海马神经元形态结构，调节炎性因子水平。这为电针治疗CFS提供了实验依据，也为CFS的机制研究提供了新的视角。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the changes of behavior and hippocampal inflammatory factors in rats with chronic fatigue syndrome （CFS）， so as to explore its possible mechanisms in the treatment of CFS.MethodsTwenty-seven SD rats were randomly divided into control， model and electroacupuncture （EA） groups （n=9 rats in each group）. The CFS model was established by multi-factor compound stress stimulation method. Rats of the EA group received EA （10 Hz） at “Shenting” （GV24） penetrating “Baihui” （GV20）， “Dazhui” （GV14） for 15 min， twice a day for 14 days. The general conditions， Morris water maze test， open field test， the exhausted running platform were conducted for determining the rats’ locomotor and learning-memory activities. H.E. staining was used to observe the morphological structure of neurons in hippocampal CA1 region. The contents of interleukin （IL）-10， IL-17 and transforming growth factor （TGF） β1 in hippocampus and serum of rats were detected by ELISA， and the positive expressions of IL-10， IL-17 and TGF-β1 in hippocampal CA1 region were detected by immunofluorescence staining.ResultsCompared with the control group， the score of general condition was increased （P<0.05）， the escape latency was prolonged （P<0.05）， the number of crossing the original platform was decreased （P<0.05）， the numbers of crossing the grid and entering the central area were increased （P<0.05）， and the exhaustive treadmill time was shortened （P<0.05） in the model group. The contents of IL-10 in the hippocampus and serum were decreased （P<0.05）， while IL-17 and TGF-β1 contents were increased （P<0.05）. The immunofluorescence intensity of IL-10 in the hippocampus was decreased （P<0.05）， while the intensity of IL-17 and TGF-β1 were increased （P<0.05）. After treatment， compared with the model group， the score of general condition was decreased （P<0.05）， the escape latency was shortened （P<0.05）， the number of crossing the original platform was increased （P<0.05）， the numbers of crossing the grid and entering the central area were decreased （P<0.05）， and the exhaustive treadmill time was prolonged （P<0.05） in the EA group. The contents of IL-10 in the hippocampus and serum were increased （P<0.05）， while IL-17 and TGF-β1 levels were decreased （P<0.05）. The immunofluorescence intensity of IL-10 in the hippocampus was increased （P<0.05）， while the intensity of IL-17 and TGF-β1 were decreased （P<0.05）. H.E. staining showed that in the model group， the number of neurons in the hippocampus decreased， with disordered arrangement and loose structure， and a small numbers of neuronal nuclei were missing. The degree of tissue damage of the EA group was milder than that of the model group.ConclusionEA can alleviate fatigue and spatial learning and memory impairment in CFS rats， which may be related to the regulation of peripheral and central inflammation.关键词：Chronic fatigue syndrome;Electroacupuncture;Inflammatory factor66|3|2更新时间：2024-05-09
- “科技新闻播报：最新研究显示，基于“气机升降”理论的经皮穴位电刺激（TEAS）治疗，能有效缓解全麻腹腔镜胆囊切除术后患者的胃肠功能紊乱。研究团队通过对患者实施不同组方选穴原则的TEAS，发现该治疗方法能降低术后早期交感神经兴奋，维持副交感神经紧张，进而促进胃肠功能恢复。这一发现为改善全麻手术后的胃肠功能问题提供了新的治疗思路。”摘要：ObjectiveTo observe the therapeutic effect of transcutaneous electrical acupoint stimulation （TEAS） based on the theory of "qi ascending and descending movement" in patients after general anesthesia laparoscopic cholecystectomy， so as to explore the impact of TEAS on the autonomic nervous system and gastrointestinal function of patients.MethodsA total of 204 patients scheduled to undergo general anesthesia laparoscopic cholecystectomy were selected and randomly divided into control， double acupoints and multiple acupoints groups， with 68 cases in each group. For patients in the multiple acupoints group， TEAS was applied at Zusanli （ST36）， Tiantu （CV22）， Danzhong （CV17）， Zhongwan （CV12）， Taichong （LR3）， and Neiguan （PC6） 30 min before anesthesia induction until the end of the surgery. In the double acupoints group， TEAS was applied only at ST36 and PC6. No electrical stimulation was applied in the control group. The postoperative bloating， bowel sound recovery time， first farting time， first defecation time， length of hospital stay， nausea and vomiting were compared among the three groups. Heart rate variability was monitored by twelve-lead electrocardiogram to evaluate the autonomic nervous function of the patients， including the low frequency power/high frequency power ratio （LF/HF）， the standard deviation of all sinus RR intervals （SDNN）， and the root mean square of difference between successive normal RR intervals （RMSSD）.ResultsAt 6 h and 24 h after surgery， the symptoms of bloating， nausea and vomiting in the multiple acupoints group and double acupoints group were significantly improved compared to the control group （P<0.05）， and the multiple acupoints group was superior to the double acupoints group （P<0.05）. Compared with the control group， the bowel sound recovery time， first farting time， first defecation time， and length of hospital stay were significantly shorter （P<0.05） in the multiple acupoints group and double acupoints group， and the multiple acupoints group was superior to the double acupoints group （P<0.05）. At 1 d and 2 d after surgery， compared with the control group， LF/HF was decreased （P<0.05） while SDNN and RMSSD were increased （P<0.05） in the multiple acupoints group and double acupoints group， and there was a significant difference between the two groups （P<0.05）.ConclusionTEAS treatment based on the theory of “qi ascending and descending movement” can relieve gastrointestinal dysfunction， reduce early postoperative sympathetic nerve excitement and maintain parasympathetic nerve tension in patients after general anesthesia laparoscopic cholecystectomy， thereby promoting gastrointestinal function recovery.关键词：Transcutaneous electrical acupoint stimulation;Laparoscopic cholecystectomy;Qi ascending and descending movement;Gastrointestinal function;Autonomic nervous system89|6|1更新时间：2024-05-09
- “近期研究观察了芒针透刺联合体针治疗中风后偏瘫伴中枢性疼痛的效果。结果显示，这种治疗方法能有效减轻疼痛，改善患者的平衡功能和肌力，促进神经功能恢复。其机制可能与抗氧化损伤有关。这一发现为中风后偏瘫伴中枢性疼痛的治疗提供了新的解决方案，有望为患者带来更好的康复效果。”摘要：ObjectiveTo investigate the effects of the elongated needling at the points of hand and foot yang meridians and the Governor Vessel combined with the routine acupuncture therapy on pain， balance function and muscle strength of the patients with post-stroke hemiplegia and central post-stroke pain （CPSP）， and to investigate whether its therapeutic mechanism is related to antioxidant damage.MethodsNinety-four patients with post-stroke hemiplegia and CPSP admitted from March 2020 to September 2021 were divided into a trial group （47 cases， 1 cases dropped out） and a control group （47 cases 3 cases dropped out）. In the control group， the rehabilitation exercise combined with routine acupuncture therapy was used， and in the trial group， on the base of the treatment as the control group， the elongated needling at the points of hand and foot yang meridians and the Governor Vessel was supplemented. In the two groups， the treatment was given once daily， and 1 course of treatment was composed of 14 days， a total of 6 courses were required in the trial. Separately， before treatment， and 1， 2 and 3 months after treatment， between two groups， the score of visual analogue scale （VAS） and that of Berg balance scale （BBS）， as well as muscle strength were compared； the neural function was evaluated using the national institutes of health stroke scale （NIHSS） and the serum contents of nitricoxide synthase （NOS）， superoxide dismutase （SOD） and malondialdehyde （MDA） were detected by ELISA in the patients.ResultsCompared with those before treatment， VAS score and NIHSS score were all decreased （P<0.05） in the trial and the control group after 1 month， 2 months and 3 months of treatment， and BBS score was increased （P<0.05）； and the case proportion of muscle strength grade 4 and 5 was higher （P<0.05） in the trial group. In the control group， the proportion of grade 4 increased after treatment for 2 months （P<0.05）， and that of grade 4 and 5 increased after treatment for 3 months （P<0.05）. The serum contents of NOS and SOD were increased （P<0.05）， and MDA was decreased （P<0.05） after 3 months of treatment in the two groups. In comparison with the control group at the same time point， VAS score and NIHSS score were lower （P<0.05）， BBS score higher （P<0.05） and the muscle strength grade was improved （P<0.05，P<0.01） after 1， 2 and 3 months of treatment， respectively； and the serum contents of NOS and SOD increased （P<0.05）， and MDA decreased （P<0.05） after 3 months of treatment in the trial group.ConclusionThe elongated needling at the points of hand and foot yang meridians and the Governor Vessel， combined with the routine acupuncture therapy alleviates CPSP， improves balance and muscle strength and promotes the recovery of neural function in the patients with post-stroke hemiplegia， the mechanism may be related to antioxidant damage.关键词：Stroke;Hemiplegia;Elongated needling;Routine acupuncture therapy;Balance function;Muscle strength;Central post-stroke pain141|26|0更新时间：2024-05-09
- “最新研究表明，芒针治疗湿热瘀阻证ⅢB型前列腺炎取得了显著疗效。试验组在接受芒针治疗后，中医证候评分、慢性前列腺炎症状指数（NIH-CPSI）评分以及疼痛症状和生活质量评分均显著降低，有效率高达88.37%，显著高于对照组的63.64%。这一发现为前列腺炎的治疗提供了新的思路和方法，展现了芒针在中医临床应用的潜力。”摘要：ObjectiveTo observe the clinical efficacy in patients with ⅢB prostatitis of stagnant dampness-heat syndrome treated with elongated needle therapy.MethodsA total of 90 patients with ⅢB prostatitis of stagnant dampness-heat syndrome were selected and randomly divided into a treatment group（45 cases， 2 dropped out） and a control group（45 cases， 1 dropped out） using a random number table method. The control group was treated with oral administration of Qianlie Shutong Capsule， 3 capsules per dose， 3 times a day for a total of 4 weeks. The treatment group received elongated needle therapy at Qihai（CV6）， Zhongji（CV3）， bilateral Zhibian（BL54） and Shuidao（ST28）， with one treatment per day for 4 weeks. Before and after treatment， the traditional Chinese medicine syndrome score， National Institutes of Health Chronic Prostatitis Symptom Index（NIH-CPSI） score were compared between the two groups， and the clinical efficacy was compared after treatment.ResultsCompared with that before treatment， the control group showed significant decrease in traditional Chinese medicine syndrome score， testicular pain， urinary frequency， unending remnants of urine， scrotal wetness， yellowish urine， urinary symptoms， and quality of life scores after treatment（P<0.05）； the treatment group showed significant decrease in traditional Chinese medicine syndrome score， and perineal pain， groin pain， pelvic pain， testicular pain， scrotal wetness， yellowish urine scores， NIH-CPSI total score， pain symptoms， urinary symptoms， and quality of life scores after treatment（P<0.05）. After treatment， the treatment group showed significantly lower traditional Chinese medicine syndrome score， and perineal pain， groin pain， pelvic pain， testicular pain scores， NIH-CPSI total score， pain symptoms， and quality of life scores than those of the control group（P<0.05）. The effective rate in the treatment group （63.64%， 28/43） was significantly higher than that in the control group （88.37%， 38/44， P<0.05）.ConclusionElongated needle therapy can significantly improve the traditional Chinese medicine syndrome score， NIH-CPSI total score， and pain symptom scores in patients with ⅢB prostatitis of stagnant dampness-heat syndrome. It can significantly improve the cure rate in these patients and is particularly effective in relieving pain.关键词：Elongated needle;Chronic prostatitis;Stagnant dampness-heat syndrome;Qianlie Shutong Capsule37|2|0更新时间：2024-05-09
- “近15年文献回顾显示，针刺对调控脑缺血再灌注损伤后的钙超载机制具有重要作用。通过抑制连接蛋白Cx43半通道开放、调控细胞内游离钙浓度、抑制钙调蛋白表达及阻断L-型电压门控钙通道功能，针刺能够拮抗神经细胞损伤，保护脑缺血再灌注后的神经功能。这一发现为缺血性中风的治疗提供了新的思路和方法。”摘要：Ischemic stroke is currently the most common type of stroke， and the key pathological link is cerebral ischemia-reperfusion injury （CIRI）， while the key factor leading to apoptosis and necrosis of ischemic nerve cells is calcium overload. Current studies have confirmed that acupuncture therapy has a good modulating effect on calcium homeostasis and can reduce cerebral ischemia-reperfusion induced damage of neuronal cells by inhibiting calcium overload. After reviewing the relevant literature published in the past 15 years， we find that acupuncture plays a role in regulating the pathological mechanism of calcium overload after CIRI by inhibiting the opening of connexin 43 hemichannels， regulating the intracellular free calcium ion concentration， suppressing the expression of calmodulin， and blocking the function of L-type voltage-gated calcium channels， thereby inhibiting calcium overload， regulating calcium homeostasis and antagonizing neuronal damage resulted from cerebral ischemia-reperfusion， which may provide ideas for future research.关键词：Acupuncture;Cerebral ischemia-reperfusion injury;Calcium overload;Nerve cell87|8|0更新时间：2024-05-09
- “针灸在减轻氧化应激反应中的作用备受关注。本文综述了近五年来针灸改善氧化应激相关机制的文献，从调节活性氧产生、影响抗氧化酶途径、修复受损分子及抑制细胞凋亡等方面，深入探讨了针灸与氧化还原生物学过程的联系。这些研究为针灸改善氧化应激及相关疾病的机制提供了新思路和依据。”摘要：Oxidative stress is associated with a variety of disease pathologies， it may lead to mitochondrial dysfunction， damage of impaired DNA repairment， cell damage or apoptosis. Acupuncture and moxibustion therapy has been proved to have a role in reducing oxidative stress in organisms under pathological conditions. In the present paper， we collected literatures in both English and Chinese from domestic and foreign databases on the mechanisms of acupuncture and moxibustion underlying amelioration of oxidative stress over the past 5 years， and analyzed the link between acupuncture-moxibustion intervention and redox biology from four aspects： 1） regulation of the production of reactive oxygen species （ROS）， 2） affecting antioxidant enzyme-related pathways to reduce ROS， 3） repairing proteins， lipids and DNAs attacked by ROS， and 4） inhibiting downstream apoptosis or autophagy of ROS pathway. By summarizing and prospecting such antioxidant mechanisms， this paper may provide some basis and ideas for in-depth mechanism research on acupuncture and moxibustion to improve oxidative stress and related diseases.关键词：Acupuncture-moxibustion;Oxidative stress;Reactive oxygen species;Antioxidant enzyme;Apoptosis64|6|0更新时间：2024-05-09
- “针灸治疗炎性肠病（IBD）的选穴和腧穴配伍规律得到了深入研究。研究团队通过检索相关文献，建立了针灸治疗IBD的数据库，并运用复杂网络技术进行分析。研究发现，针灸治疗IBD的选穴原则以胸腹部局部选穴为主，同时注重远近配穴，特定穴中的募穴应用广泛。此研究为针灸治疗IBD的选穴提供了重要参考。”摘要：ObjectiveTo analyze the rules of acupoint selection and compatibility of acupuncture and moxibustion in treatment of inflammatory bowel disease （IBD） based on complex network technology and provide the reliable evidences for acupoint selection in treatment of this disease with acupuncture and moxibustion.MethodsThe clinical studies on acupuncture-moxibustion treatment of IBD were searched from the databases including CNKI， Wanfang， VIP， PubMed and Embase. The studies were screened and the acupoint prescriptions were extracted to set up the database of acupuncture-moxibustion treatment for IBD. Using Microsoft Excel 2021 software， the use times of acupoint， the use frequency （%） of acupoint， meridian tropism and the use of special point were imported. With SPSS Modeler 18.0 software adopted， the association rules were analyzed on the acupoint prescriptions. The acupoint co-occurrence network diagram， k-core network diagram， and community analysis diagram were drawn by Gephi 0.9.5 software.ResultsA total of 156 studies were included， composed of 175 acupoint prescriptions， 75 acupoints， with 1 378 use times in total and around 8 acupoints in one prescription. Regarding the top use frequency， Tianshu （ST25）， Zusanli （ST36）， Guanyuan （CV4）， Zhongwan （CV12） and Pishu （BL20） were listed. The top meridians involved were the foot-yangming stomach meridian， the foot-taiyang bladder meridian and the Conception Vessel. The front-mu point had been used with the highest frequency among the special points. ST36 and ST25 were a pair of points with the highest frequency in treatment. The k-core hierarchical analysis was adopted to optimize acupoint prescriptions， and 22 core acupoints were obtained， i.e. ST25， ST36， CV4， CV12， BL20， Dachangshu （BL25）， Shangjuxu （ST37）， Shenshu （BL23）， Qihai （CV6）， Sanyinjiao （SP6）， Mingmen （GV4）， Xingjian （LR2）， Yinlingquan （SP9）， Neiting （ST44）， Taichong （LR3）， Xiajuxu （ST39）， Shuifen （CV9）， Shenque （CV8）， Ganshu （BL18）， Weishu （BL21）， Hegu （LI4） and Quchi（LI11）， which were classified into three core acupoint groups by community analysis.ConclusionThrough complex network analysis， it is found that the local acupoints on the chest and abdomen are generally selected in treatment with acupuncture-moxibustion for IBD， the combination of the nearby and distal points is considered simulta-neously， and the acupoint prescription is modified according to syndrome/pattern differentiation； and among special points， the front-mu point is widely used in treatment. All of these rules provide the ideas for the acupoint selection of acupuncture-moxibustion in treatment of IBD.关键词：Inflammatory bowel disease;Acupuncture and moxibustion;Complex networks;Community analysis73|7|0更新时间：2024-05-09
- “《Acupuncture Anesthesia in the People’s Republic of China》是中国针刺麻醉史上的首份域外系统考察报告，尽管当时未引起国内广泛关注，但其在针刺麻醉发展史上具有重要意义。报告涉及了针刺麻醉的临床观察、疗效因素分析和中外研究差异，为现代针刺麻醉的发展和完善提供了宝贵借鉴。通过回顾这份报告，我们能够更好地理解针刺麻醉的历史演变和未来发展潜力，为针灸医学和生命科学的研究提供新的启示。”摘要：“Acupuncture Anesthesia in the People's Republic of China： A Trip Report of the American Acupuncture Anesthesia Study Group” （Report for short） is the first extraterritorial systematic investigation and evaluation report in the history of acupuncture anesthesia in China. After Report was released to the public， it has not drawn much attention in China. At the moment when acupuncture anesthesia is extended to perioperative treatment， by reviewing the Report， the differences in understanding acupuncture anesthesia were analyzed from a foreign perspective. Report involves the clinical observation of surgery under acupuncture anesthesia， the analysis on the factors of acupuncture anesthesia in therapeutic effects and the relevant mechanisms， and the differences in the research methods and foci on the recognition of acupuncture anesthesia between China and foreign countries. In association with the development of acupuncture anesthesia during the past 65 years and the conclusion in Report， the paper briefly describes the development and perfection of acupuncture anesthesia， the historical verification to the conclusions in Report and the limitations in modern acupuncture anesthesia. It provides some inspiration for the development of acupuncture anesthesia， acupuncture-moxibustion medicine and life science in the future.关键词：Acupuncture anesthesia;A foreign perspective;“Acupuncture Anesthesia in the People's Republic of China”;Book review43|7|0更新时间：2024-05-09
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