最新刊期
卷 49 ， 期 9 ， 2024
- “在心肌缺血动物模型中，体表穴位敏化与垂体腺苷酸环化酶激活多肽表达关系的研究取得进展，为穴位敏化机制研究提供新思路。”摘要：ObjectiveTo explore the relationship between sensitization points of the body surface and the expression of pituitary adenylate cyclase activating polypeptides （PACAP） in myocardial ischemia （MI） mice， so as to reveal the underlying mechanisms of acupoint sensitization from the perspective of molecular biology.MethodsMale C57BL/6J mice were randomly divided into control and model groups （28 mice/group）. The MI-induced visceral pain model was established by intraperitoneal injection of isoprenaline （ISO， 160 mg/kg）. The mice of the control group received intraperitoneal injection of the same dose of normal saline. Six days after modeling， the Evans blue （EB） dye was injected into the tail vein of mice to observe the distribution and quantity of the plasma extravasated EB points at the body surface. Meanwhile， the mechanical pain threshold （MPT） was measured to evaluate the level of pain sensitivity in the activated area on their body surface and left forelimb and hindlimb， respectively. Hematoxylin-eosin （H.E.） staining was used to evaluate the morphologic and pathological changes of the heart tissue in the two groups. Then， the expressions of PACAP in the thoracic （T）1—T5 dorsal root ganglia （DRGs）， spinal cord and skin in the dominant area of body surface were detected by Western blot and immunofluorescence staining， respectively.ResultsCompared with the control group， the heart tissue of the model group was hypertrophic and the myocardial tissue showed obvious inflammatory cell infiltration and fibrosis. In addition to these pathologic changes， the number of EB exudation points on the body surface was significantly increased （P<0.01）， and was mainly distributed in the innervated region of T1—T5 segments of the spinal cord， and the MPT of these EB exudation points was lower than that of non-exudation points （P<0.01）. At the same time， the MPTs of left forelimb and hindlimb were significantly decreased in the model group （P<0.001）. More importantly， the level of protein and positive expression of PACAP were significantly higher in the model group than those in the control group， which was observed in the innervated body surface， spinal cord and its DRG neurons of T1—T5 segments （P<0.01， P<0.001， P<0.05）.ConclusionISO injection resulted in histological lesions and cardiogenic referred pain on the body surface after the formation of MI in mice. The expression of PACAP in the body surface of the sensitization points， the corresponding T1—T5 segments of spinal cord and DRG neurons were significantly increased， which may partly explain the reason for acupoint sensitization in the animal model of visceral pain.关键词：Myocardial ischemia;Acupoint sensitization;Pituitary adenylate cyclase activating polypeptide106|6|0更新时间：2024-12-05
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Electroacupuncture promotes angiogenesis in MIRI rats by modulating the AMPK/KLF2 signaling pathway AI导读
“最新研究发现，电针通过调节AMPK/KLF2信号通路，促进血管新生，减轻心肌缺血再灌注损伤，具有心肌保护效应。”摘要：ObjectiveTo observe the effect of electroacupuncture（EA） on adenosine 5’-monophosphate-activated protein kinase （AMPK）/Kruppel-like factor 2 （KLF2） signaling pathway in ischemic myocardial tissues of rats， so as to explore the underlying mechanism of EA in attenuating myocardial ischemia-reperfusion injury （MIRI） through mediating angiogenesis.MethodsMale SD rats were randomly divided into sham operation group， model group and EA group， with 10 rats in each group. The MIRI model was established by ligation of the anterior descending branch of the left coronary artery. Twenty-four hours after modeling， the rats in the EA group were given EA （2 Hz/100 Hz， 2 mA） at “Neiguan” （PC6） for 20 min each time， once a day for 5 consecutive days. Echocardiography was used to detect cardiac ejection fraction （EF） to evaluate cardiac function. HE staining was used to observe the morphological changes in rat myocardial tissue. Immunohistochemistry was used to detect the density of neovascularization in rat ischemic myocardium. Western blot and ELISA were used to detect the phosphorylated（p）-AMPK， AMPK， KLF2， vascular endothelial growth factor （VEGF） protein expression levels， and VEGF receptor 2 （VEGFR2） content in rat ischemic myocardial tissue， respectively.ResultsAfter modeling， compared with the sham operation group， rats in the model group had decreased EF（P0.01）， significant myocardial fiber damage with inflammatory cell infiltration， increased neovascular density （P0.05）， increased p-AMPK， AMPK， VEGF protein expression levels and VEGFR2 content in myocardial ischemic tissues（P0.05，P0.01）， and decreased protein expression level of KLF2 （P0.05）. After EA intervention， compared with the model group， rats in the EA group had elevated EF（P0.01）， significantly reduced myocardial fiber damage， reduced inflammatory cell infiltration， increased neovascular density（P0.01）， and elevated p-AMPK， AMPK， KLF2， and VEGF protein expression levels and VEGFR2 content in the myocardial ischemic tissue （P0.01）.ConclusionEA may promote angiogenesis， attenuate myocardial injury， and achieve cardioprotective effects in MIRI rats by regulating the expression of AMPK/KLF2 signaling pathway in myocardial tissues.关键词：Electroacupuncture;Myocardial ischemia-reperfusion injury;Angiogenesis;AMPK/KLF2 signaling pathway;Vascular endothelial growth factor77|5|1更新时间：2024-12-05 - “最新研究发现，电针对神经根型颈椎病大鼠具有镇痛效果，可能通过抑制星形胶质细胞活化及HMGB1/TLR4/MyD88信号通路，减少相关细胞因子释放，缓解脊髓节段的中枢敏化。”摘要：ObjectiveTo observe effects of electroacupuncture （EA） on the activation of astrocytes and high mobility group protein B1（HMGB1）/Toll-like receptor 4 （TLR4）/myeloid differentiation factor 88 （MyD88） signaling pathway， as well as related cytokines in rats with cervical spondylosis radiculopathy（CSR）， so as to explore the analgesic mechanism of EA in treating CSR.MethodsTwenty-four male SD rats were randomly divided into blank， sham surgery， model， and EA groups， with 6 rats in each group. CSR rat model was established by using cervical spinal cord canal puncture method. On the 7th day after successful modeling， EA was applied to rats in the EA group at bilateral “Hegu”（LI4） and “Taichong”（LR3） for 20 minutes（1.5 Hz， 1 mA）， once daily for 7 consecutive days. Before and after intervention， gait impairment scores and mechanical pain thresholds were assessed. HE staining was used to observe pathological changes in spinal cord tissue. Western blot was used to detect the expression of HMGB1， TLR4， MyD88， and glial fibrillary acidic protein （GFAP） in the spinal cord. ELISA was used to measure the contents of CXC chemokine ligand 1 （CXCL1）， chemokine ligand 2 （CCL2）， tumor necrosis factor （TNF）-α， and interleukin （IL）-1β in spinal cord. Immunofluorescence staining was used to observe GFAP protein positive expression in spinal cord tissue.ResultsThere was no significant difference of all indexes between the blank group and the sham surgery group. Compared with the sham surgery group， mechanical pain threshold of rats in the model group was decreased（P0.01）， while gait impairment score， the contents of CXCL1， CCL2， TNF-α， IL-1β， protein expressions of HMGB1， TLR4， MyD88 and GFAP， and positive expression of GFAP in spinal cord tissue were increased （P0.01）； HE staining indicated severe overall morphological damage in the spinal cord of rats in the model group， with significant shrinkage of gray matter neurons， reduced number of Nissl bodies， and increased inflammatory cell infiltration. Compared with the model group， mechanical pain threshold in the EA group was increased （P0.01）， while gait impairment score， the contents of CXCL1， CCL2， TNF-α， IL-1β， protein expressions of HMGB1， TLR4， MyD88 and GFAP， and positive expression of GFAP in spinal cord were reduced （P0.01）； HE staining showed more intact neuronal cell bodies， increased number of Nissl bodies， and reduced shrinkage of gray matter neurons， inflammatory cell infiltration， and microvascular dilation in the spinal cord of rats in the EA group.ConclusionEA can effectively alleviate pain in CSR rats， which is possibly by inhibiting astrocyte activation， HMGB1/TLR4/MyD88 signaling pathway， and reducing the release of related inflammatory cytokines， thus alleviating central sensitization in spinal segments.关键词：Cervical spondylosis radiculopathy;Electroacupuncture;Astrocyte;Chemokine;Inflammatory cytokine;Central sensitization;High mobility group protein B1/Toll-like receptor 4/myeloid differentiation factor 88 signaling pathway82|17|0更新时间：2024-12-05
- “最新研究发现，模拟经颅重复针刺（rTAS）手法能显著改善血管性痴呆大鼠的学习记忆能力，增加大脑血流灌注，可能通过促进血管新生、改善内皮功能、抗氧化应激发挥作用。”摘要：ObjectiveTo observe the effect of simulated repeated transcranial acupuncture （rTAS） on learning and memory abilities and cerebral microvascular flow in vascular dementia （VD） model rats， so as to explore the potential mechanism of rTAS in treating VD.MethodsThirty-two Wistar rats were randomly divided into normal， model， acupuncture and rTAS groups （n=8 rats in each group）. The VD model was established by permanent ligation of bilateral common carotid arteries. For rats of the acupuncture group， “Baihui” （GV20） and “Shenting” （GV24） were needled， and for rats of the rTAS group， GV20 and GV24 were stimulated by simulated repeated transcranial manipulation （200 r/min， for 5 min）. The treatment was conducted once daily for 14 days. After the intervention， learning and memory abilities were evaluated using the Morris water maze test. Laser speckle technology was used to measure the average cerebral microvascular flow. ELISA was performed to measure the contents of vascular endothelial growth factor （VEGF）， endothelin-1 （ET-1）， nitric oxide （NO）， and inducible nitric oxide synthase （iNOS） in the hippocampal tissues.ResultsIn comparison with the normal group， the escape latency of rats in the model group was prolonged （P0.01）， and the times of crossing the platform were decreased （P0.01）. The average cerebral microvascular flow and the VEGF content in the hippocampus were significantly decreased， while the contents of NO， iNOS， and ET-1 were significantly increased （P0.01）. In comparison with the model group， the escape latency was significantly shortened （P0.01）， the average cerebral microvascular flow and VEGF content in the hippocampus were significantly increased （P0.05，P0.01）， while contents of iNOS were significantly decreased （P0.05， P0.01） in both acupuncture and rTAS groups； and the times of crossing the platform were increased （P0.01）， the contents of NO and ET-1 in hippocampus were significantly decreased （P0.01） in the rTAS group. The effects of rTAS were significantly superior to those of acupuncture in up-regulating the average cerebral microvascular flow （P0.05） and VEGF content （P0.01）， and down-regulating the NO， iNOS and ET-1 contents （P0.01， P0.05）.ConclusionrTAS can increase cerebral microvascular flow， improve spatial cognition and enhance learning and memory abilities of VD rats. The underlying mechanism may be involved in promoting angiogenesis， improving endothelial function and mitigating oxidative stress.关键词：Repeated transcranial acupuncture manipulation;Vascular dementia;Microvascular flow;Laser speckle;Learning and memory ability58|2|0更新时间：2024-12-05
- “最新研究发现，温和灸能降低肠易激综合征模型大鼠内脏高敏感性，改善腹痛腹泻症状，可能通过下调SCF/c-kit信号通路表达实现。”摘要：ObjectiveTo observe the effect of mild moxibustion on stem cell factor （SCF）/tyrosine kinase receptor （c-kit） signaling pathway and visceral hypersensitivity of diarrhea predominant irritable bowel syndrome （IBS-D） model rats with liver-qi stagnation and spleen deficiency syndrome， so as to explore its mechanisms underlying improvement of IBS-D.MethodsA total of 24 male Wistar rats were randomly divided into normal， IBS-D model， medication and mild moxibustion groups， with 6 rats in each group. The IBS-D model was established by glacial acetic acid （4%） enema plus restraint stress stimulation once daily for 14 days. Rats of the medication group were treated by gavage of pivamium bromide （15 mg/kg） once a day for 14 days. Mild moxibustion was applied to bilateral “Tianshu”（ST25）， “Shangjuxu”（ST37） and “Taichong”（LR3） for 20 min， once daily for 14 consecutive days. After the intervention， the rats’ general state of each group were observed. The rate of loose stools （LSR）， and the minimum volume threshold for abdominal withdrawal reflex（AWR） were observed， and the open field test was used to assess the state of rats’ motor activities （including rearing times， grooming times and total number of square-crossings in 5 min）. Morphological changes of the colon tissue were observed by hematoxylin-eosin （H.E.） staining， The count of mast cells （MC） in the colon tissues was determined by toluidine blue staining. Contents of serum 5-hydroxytryptamine （5-HT） and substance P （SP） were determined by enzyme-linked immunosorbent assay （ELISA）. The relative expression levels of SCF and c-kit mRNAs and proteins in the colon tissues were detected by real-time quantitative PCR and Western blot， respectively.ResultsCompared with the normal group， the body weight， minimum volume threshold of AWR， total numbers of square-crossing， rearing times and grooming times were significantly decreased （P0.05）， and the LSR， number of MC， contents of 5-HT and SP， and the expression levels of SCF and c-kit mRNAs and proteins were considerably increased in the model group （P0.01）. In comparison with the model group after interventions， the body weight， minimum volume threshold of AWR， total numbers of square-crossing， rearing times and grooming times were apparently increased in both medication and moxibustion groups （P0.05， P0.01）， and the LSR， number of MC， 5-HT and SP contents in both medication and moxibustion groups， and the expression levels of SCF and c-kit mRNA and protein in the moxibustion group （not in the medication group） were obviously decreased （P0.05， P0.01）. H.E. staining showed that in the model group， a small amount of inflammatory cells in the mucosal layer of colon tissue could be seen. in the medication group， a small number of lymphocytes in colon tissue were observed， while in the mild moxibustion group， a small amount of neutrophils in colon tissue were observed.ConclusionMild moxibustion can reduce visceral hypersensitivity and improve abdominal pain， diarrhea and locomotion state in IBS-D rats with liver-qi stagnation and spleen deficiency syndrome， which may be associated with its functions in reducing the number of MC and the levels of 5-HT and SP and down-regulating the activities of SCF/c-kit signaling pathway.关键词：Mild moxibustion;Diarrhea predominant irritable bowel syndrome （IBS-D）;Liver-qi stagnation and spleen deficiency syndrome;Stem cell factor/tyrosine kinase receptor （SCF/c-kit） signaling;Visceral hypersensitivity129|9|0更新时间：2024-12-05
- “最新研究发现，“秩边透水道”针法能改善早发性卵巢功能不全大鼠的卵巢功能，其机制可能与激活SIRT1/PGC-1α/Nrf2信号通路有关。”摘要：ObjectiveTo observe the effect of penetration needling from “Zhibian” （BL54） to “Shuidao”（ST28） on silencing information regulator 1 （SIRT1） /peroxisome proliferator-activated receptor-γ co-activator 1α （PGC-1α） /nuclear factor E2 related factor 2 （Nrf2） signaling in rats with premature ovarian insufficiency （POI）， so as to explore its mechanisms underlying improvement of POI.MethodsA total of 48 female SD rats were equally and randomly allocated to blank control， POI model， shallow needling and penetration needling （from “Zhibian” ［BL54］ to “Shuidao” ［ST28］） groups. The POI model was established by intraperitoneal injection of cyclophosphamide （50 mg·kg-1·d-1 on the 1st day and 8 mg·kg-1·d-1 from the 2nd to 15th day， for a total of 15 days）. After successful modeling， for rats of the shallow needling group， a filiform needle was inserted into BL54 to a depth about 5—8 mm， and then retained for 30 min. And for rats of the penetration needling group， a filiform needle was inserted into BL54 area and advanced to the unilateral ST28 to a depth about 12—15 mm， and then retained for 30 min （bilateral acupoints were used at the same time）. The treatments were conducted once daily， 6 times a week for 4 weeks. After the interventions， the contents of serum follicle stimulating hormone （FSH）， luteinizing hormone （LH）， estradiol （E2） and anti-Müllerian hormone （AMH） were detected using ELISA， and the activity of superoxide dismutase （SOD）， catalase （CAT） and content of malondialdehyde （MDA） in the ovarian tissue were detected using colorimetry. Histopathological changes of the ovarian tissue were observed after H.E. staining. The immunoactivities and expression levels of SIRT1， PGC-1α， and Nrf2 mRNA and protein in the ovarian tissues were detected using immunohistochemistry， quantitative real-time PCR and Western blot， respectively.ResultsAfter modeling， the rats’ estrus cycles were disordered， contents of serum FSH and LH levels significantly increased， and the E2 level markedly decreased compared with those of the blank control group （P0.01）， indicating that the POI model was successfully established. Relevant to the blank control group， the model group had an increase in serum FSH and LH， ovarian MDA contents， and the number of atretic oocytes （P0.01）， and a decrease in serum E2 and AMH contents， ovarian SOD and CAT activities， number of growing oocytes， immunoactivities and expressions of ovarian SIRT1， PGC-1α and Nrf2 protein and mRNA （P0.01， P0.05）. Following interventions， both the increased levels of serum FSH and LH and ovarian MDA contents， and the number of atretic oocytes， and the decreased levels of E2 and AMH contents， ovarian SOD and CAT activities， number of growing oocytes， immunoactivities and expressions of ovarian SIRT1， PGC-1α and Nrf2 protein and mRNA were reversed by penetration needling of BL54—ST28 （P0.01， P0.05）， but not by shallow needling， except serum FSH， LH， E2 and AMH contents. The effects of penetration needling were obviously superior to those of shallow needling in up-regulating the levels of serum AMH， ovarian SOD and CAT， number of growing oocytes， and the expressions of ovarian SIRT1， PGC-1α and Nrf2 protein and mRNA （P0.05， P0.01）， and in down-regulating the level of MDA and the number of atretic oocytes （P0.05）.ConclusionPenetration needling stimulation of BL54 to ST28 can increase the number of ovarian growing oocytes and reduce the number of atretic oocytes， regulate the serum hormone levels and relieve the ovarian oxidative stress level in POI rats，which may be associated with its functions in activating ovarian SIRT1/PGC-1α/Nrf2 signaling pathway.关键词：Premature ovarian insufficiency;Penetration needling from “Zhibian” （BL54） to “Shuidao” （ST28）;Oxidative stress;SIRT1/PGC-1α/Nrf2 signaling pathway69|8|0更新时间：2024-12-05
- “最新研究发现，针刺能有效缓解类风湿关节炎小鼠的关节炎性反应，可能通过调节肠道菌群多样性和结构组成发挥作用。”摘要：ObjectiveTo observe the effect of acupuncture on gut microbiota in mice with rheumatoid arthritis （RA）， so as to explore its mechanisms underlying improvement of RA.MethodsEighteen male C57BL/6J mice were randomly divided into saline， model and acupuncture groups， with 6 mice in each group. The RA model was established by injection of complete Freund's adjuvant （CFA，50 μL） into the right hind paw. Acupuncture group received manual acupuncture stimulation of the bilateral “Zusanli” （ST36） for 30 min， once daily for 7 days. The paw withdrawal thermal latency （PWTL） was detected by using a thermal pain detector. After the last intervention， the mice’s feces were collected for extracting the feces DNA， and 16S RNA sequencing was used to detect and analyze the diversity and structural composition of intestinal flora.ResultsCompared with the saline group， the PWTL from day 0 to 7 after administration of CFA was significantly decreased （P0.01）， and the paw swelling volume from day 0 to 7 significantly increased in the model group （P0.01）. In comparison with the model group， the PWTL from day 1 to 7 was significantly increased （P0.01），and the paw swelling volume on day 7 was strikingly decreased （P0.01） in the acupuncture group. The Alpha diversity analysis of intestinal flora showed that the Chao1 index was markedly decreased in the model group compared with the saline group （P0.05）， and markedly increased in the acupuncture group compared with the model group （P0.05）. Compared with the saline group， the relative abundance of Bacteroidota was decreased （P0.05）， and that of Desulfobacterota and Escherichia-Shigella significantly increased （P0.05） in the model group. In comparison with the model group， the relative abundance of Actinobacteria and Lactobacillus was considerably increased （P0.05， P0.01）， while that of Escherichia-Shigella and Faecalibacterium strikingly decreased （P0.05） in the acupuncture group.ConclusionAcupuncture treatment can significantly alleviate joint inflammation in RA mice， which may be related to its function in regulating the diversity and composition of gut microbiota.关键词：Acupuncture;Rheumatoid arthritis;Gut microbiota63|9|0更新时间：2024-12-05
- “最新研究发现，电针疗法能减轻肥胖大鼠体重，改善肠道菌群结构，降低炎症反应。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the expressions of high mobility group protein 1（HMGB1） and myeloid differentiation factor 88 （MyD88） in the small intestine and intestinal flora of obese rats， so as to explore the potential mechanism of EA to improve obesity in rats.MethodsAfter 1 week of acclimatization， 10 rats were randomly selected from 50 Wistar male rats as the normal group， and the rest rats were fed with high-fat diet for 8 weeks to establish the obese model. The successfully modeling rats were randomly divided into model group， EA group and sham EA group， with 10 rats in each group. Rats in the EA group were given EA （2 Hz， 1 mA） at “Zhongwan”（CV12）， “Guanyuan”（CV4）， “Zusanli” （ST36）and “Fenglong”（ST40）. Rats in the sham EA group were given shallow stabs at acupoints of the EA group about 5 mm outwardly and the electrodes were clamped without being energized. Both groups were intervened for 10 min each time， 3 times （Monday， Wednesday and Friday） a week for 8 weeks. The body weights of the rats were measured before and after 8 weeks of intervention， respectively. The contents of serum lipopolysaccharide （LPS） and tumor necrosis factor （TNF）-α were measured by ELISA， and the protein and mRNA expressions of HMGB1 and MyD88 in the small intestine were detected by Western blot and real-time quantitative PCR， respectively. 16S rRNA sequencing was performed to determine the relative abundance and diversity of the bacterial flora in the fresh feces of rats.ResultsCompared with the normal group， the body weight， serum LPS and TNF-α contents， small intestinal HMGB1 and MyD88 protein and mRNA expression levels of rats in the model group were significantly increased （P0.01）， while the relative abundance of Lactobacillus， Muri and Bifidobacterium was decreased （p0.01）， Collinsella， Prevotella and Ruminococcus was increased （P0.01）. Compared with model group， the body weight， serum LPS and TNF-α contents， protein and mRNA expression levels of HMGB1 and MyD88 in both EA and sham EA groups were decreased （P0.01，P0.05）， while the relative abundance of Lactobacillus， Muri and Bifidobacterium was increased （P0.01） and Collinsella， Prevotella and Ruminococcus decreased （P0.01）. Comparison between EA group and sham EA group showed that， the contents of LPS and TNF-α in serum of rats in sham EA group were increased （P0.01，P0.05）， the relative abundance of Lactobacillus， Muri and Bifidobacterium was lower （P0.05， P0.01）， and Collinsella， Prevotella and Ruminococcus was higher （P0.01）.ConclusionEA can reduce the body weight of obese rats， which may be related to the regulation of the structure of intestinal flora and the reduction of inflammatory reactions in the small intestine.关键词：Obesity;Electroacupuncture;Intestinal flora;Inflammatory reaction68|7|0更新时间：2024-12-05
- “在梨状肌综合征治疗领域，一项新研究比较了激痛点针刺与糖皮质激素注射的疗效。结果显示，激痛点针刺在缓解疼痛、改善功能和提高生活质量方面，较糖皮质激素注射更具优势。”摘要：ObjectiveTo compare the difference in the clinical efficacy on piriformis syndrome between trigger-point （TrP） acupuncture and glucocorticoid injection.MethodsSixty patients with piriformis syndrome were randomly allocated to an acupuncture group （30 cases， treated with TrP acupuncture） and a control group （30 cases， treated with glucocorticoid injection）. In the two groups， the intervention was delivered once weekly and 2 treatments were required. Before treatment and 1 week， 1 month， 2 months and 3 months after treatment， the scores of the numerical rating scale （NRS） and Oswestry dysfunction index questionnaire （ODI）， and the passive hip range of motion （PROM） were collected separately； the score of the 36-item short form of health survey （SF-36） was observed 3 months after treatment； and the administration of analgesic medication and the occurrence of adverse effects were recorded in the patients of 2 groups.ResultsThe scores of NRS and ODI were decreased， and PROM was increased at each time point compared with the baseline （before treatment） in both groups （P0.05）. In comparison with the control group， the scores of NRS and ODI were decreased （P0.01， P0.05） and the range of hip internal rotation （HIR） was increased in the acupuncture group 2 and 3 months after treatment （P0.01）. Three months after treatment， the scores for physiological function， body pain， and vitality of SF-36 in the acupuncture group were higher than those of the control group （P0.05）. The number of patients with analgesic drugs was less （P0.05） in the acupuncture group than that in the control group in 2 and 3 months after treatment. During treatment and in follow-up stage， no serious adverse reactions occurred in the patients of 2 groups.ConclusionThe clinical effect of TrP acupuncture is similar to that of glucocorticoid injection on piriformis syndrome in 1 month after treatment. In 2 months after treatment， TrP acupuncture is markedly effective for attenuating pain and the functional impairment of the lower limbs， improving the quality of life and reducing the use of analgesic drugs in comparison with glucocorticoid injection.关键词：Piriformis Syndrome;Trigger-point;Acupuncture;Glucocorticoid167|15|0更新时间：2024-12-05
- “最新研究显示，鼻内针刺联合调神针治疗中重度持续性变应性鼻炎效果显著，可改善鼻部症状，降低嗜酸性粒细胞、免疫球蛋白E水平，抑制超敏反应，同时调节神经肽分泌，缓解焦虑抑郁情绪。”摘要：ObjectiveTo observe the therapeutic effect of intranasal acupuncture combined with Tiaoshen （spirit-regulation） acupuncture for patients with moderate-to-severe persistent allergic rhinitis （AR）， and to explore its mechanism of anti-inflammation.Methods135 patients with persistent AR were randomly divided into western medicine group， intranasal acupuncture group， and combination group， with 45 cases in each group. The western medicine group was treated with budesonide nasal spray， 1 press （32 μg/press） in each nostril， once a day. Patients in the intranasal acupuncture group were treated with intranasal acupuncture at the Neiyingxiang （EX-HN9） and Biqiu （nasal hillock） for 20 min. Patients in the combination group were treated with intranasal acupuncture combined with spirit-regulation acupuncture at Baihui （GV20）， Sishencong （EX-HN1）， Daling （PC7）， Shenmen （HT7）， Yintang （GV24+）， Shenting （GV24）， Anmian， and Yingxiang （LI20） for 20 min. Each group was treated once daily for 2 weeks. Total nasal symptom score （TNSS）， total non-nasal symptom score （TNNSS）， rhinoconjunctivitis quality of life questionnaire （RQLQ）， self-assessment scale of anxiety （SAS）， and self-assessment scale of depression （SDS） were observed before and after treatment respectively. Serum total immunoglobulin E （IgE）， substance P （SP）， neuropeptide Y （NPY）， and vasoactive intestinal peptide （VIP） levels were detected before and after treatment using ELISA. The number of eosinophil （EOS） in peripheral venous blood was detected using a blood analyzer. The clincial efficacy of the 3 groups was evaluated.ResultsCompared with those before treatment， TNSS， TNNSS， RQLQ， SAS， SDS scores， EOS number and serum IgE， SP and VIP contents were decreased （P0.05）， and serum NPY content was increased （P0.05） after treatment in the 3 groups. After treatment， the observation indexes in the intranasal acupuncture group were significantly improved （P0.05） than those in the western medication group. The observation indexes of the combination group were better （P0.05） than those of the other 2 groups. The total effective rate of the combination group （40/45， 88.89%） was higher （P0.05） than that of the intranasal acupuncture group （35/45， 77.78%） and higher （P0.05） than that of the western medication group （33/45， 73.33%）.ConclusionIntranasal acupuncture combined with spirit-regulation acupuncture can improve the nasal clinical symptoms and accompanying symptoms of AR patients， reduce EOS and IgE， as well as regulate the secretion of neuropeptide and relieve the negative emotions of anxiety and depression.关键词：Intranasal acupuncture;Tiaoshen （spirit-regulation） acupuncture;Allergic rhinitis;Clinical efficacy100|13|0更新时间：2024-12-05
- “针刺腧穴抗炎效应研究取得新进展，揭示了腧穴调控炎性反应的作用机制和途径，为明确针刺腧穴调节炎性反应的作用规律提供新思路。”摘要：A large number of studies have confirmed the anti-inflammatory effects of acupuncture， and some of the mechanisms and pathways regulating inflammatory response have been revealed. However， most of these researches focused on the effect of acupuncture on systemic anti-inflammation， and there is no consensus about the effect characteristics of different acupoints on regulating inflammatory response. It is noteworthy that increasing attention and exploration have been paid to the neuro-immune interactions and regulation of immune-inflammatory homeostasis. Importantly， the understanding of local neuroimmune regulation of non-immune organs has been deepening， which was known as the regional immunity. This new concept lays a scientific foundation for elucidating the characteristics of acupoints on the inflammation， especially the modulation of target visceral organs by the relevant acupoint stimulation. In this paper， the local effects （e.g. activating regional nerve components to induce local neuroimmuno-inflammatory regulation， etc）， target visceral organ effects （e.g. regulating activities of visceral resident immune cells to initiate regional immunity regulation mediated by locally resident lymphocytes to promote inflammatory response degradation and to restore the homeostasis of regional immunity in the internal organs， via somato-visceral neuro-segmental connection， etc.） and systemic anti-inflammatory effects （e.g. regulating cholinergic anti-inflammatory pathway， including activating the vagus nerve to exert systemic anti-inflammatory effects through neuroimmune regulatory network， etc.） of acupoint stimulation were analyzed from different levels of neuroimmunological regulation， so as to provide new insights for clarifying the role of acupoints in improving inflammatory diseases.关键词：Acupoint effects;Neuro-immune interaction;Local effects;Specific target organ effects;Systemic effects99|18|1更新时间：2024-12-05
- “电头针治疗缺血性脑卒中参数研究进展，为优化治疗方案提供参考。”摘要：Scalp-electroacupuncture （scalp-EA） is an effective traditional Chinese medicine characteristic therapy for ischemic stroke and frequently employed in clinical practice， but there is still lack of normative standard of stimulation parameters. After searching the relevant articles， we summed up the stimulation parameters of scalp-EA for ischemic stroke from 5 aspects： 1） stimulating frequency （Low frequency is better when continuous waveform is used， and high frequency is better when dense-sparce waveform or discontinuous waveform employed）， 2） waveform （Dense-sparce or discontinuous waves are better than continuous waves）， 3） intensity （A tolerable stimulation strength may result in better outcomes）， 4） needle retention time （In general， the needle retention time is 30 min， but still needing being determined）， treatment course （Some studies showed that once a day EA for 3 continuous days is effective， 7 days， significantly effective， 14 days， further enhanced in the therapeutic effect）， and 5） intervention time window （Generally， EA intervention within 6 h was mostly effective， shown by animal study）. However， these parameters do not exist independently， and they can influence and interact with each other. It is difficult to observe the interaction among parameters only with single-dimension analysis. Hence， more and larger sample size studies， with strict inclusion and exclusion criteria， more reasonable research design （such as multiple factorial design， orthogonal experimental design， etc.） and multiple levels effect analysis are warranted.关键词：Scalp-electroacupuncture;Ischemic stroke;Stimulation parameters;Review59|14|0更新时间：2024-12-05
- “最新研究揭示，针刺通过改善氧化应激治疗帕金森病，为该病治疗提供新方案。”摘要：Parkinson’s disease （PD） is a neurodegenerative disease commonly seen in middle-aged and elderly people. The incidence of PD is increasing year by year， which seriously affects the patients’ quality of life. Oxidative stress （OS） is one of the pathogeneses of PD. In recent years， with the deepening of research， it has been found that OS is closely related to other PD-related pathogenesis， such as the susceptibility of substantia nigra， mitochondrial dysfunction， abnormal folding of α -synuclein （α -Syn）， nitric oxide （NO） increasing， endoplasmic reticulum stress （ERS）， decreased antioxidant capacity， etc.， which is considered to be the central link of the complex convergence of various pathogenesis. A large number of studies also have shown that acupuncture has great potential in regulating OS and treatment of PD. In the present article， we reviewed the role of OS in PD pathology and the mechanism of acupuncture in the treatment of PD by improving OS in recent years： 1） acupuncture acts on Parkinson’s disease by increasing antioxidant enzyme levels， 2） acupuncture works in Parkinson’s disease by improving pathways related to OS （Nrf2/ARE， PI3K/Akt），3） acupuncture acts on Parkinson’s disease by improving OS-related neuroinflammation，and 4） acupuncture acts on Parkinson’s disease by improving the cerebral-intestinal axis associated oxidative stress.关键词：Parkinson’s disease;Acupuncture;Oxidative stress;Research progress81|10|0更新时间：2024-12-05
- “针灸治疗银屑病研究取得新进展，分析了近20年临床文献，发现针刺联合艾灸、火针联合西药为主要干预方法，疗效显著且安全。”摘要：ObjectiveTo sum up clinical researches and the rule of acupoint selection on the treatment of psoriasis by acupuncture and moxibustion in the past 20 years， so as to provide new ideas and reference for improving the clinical effect of acupuncture for psoriasis.MethodsResearch papers， published from January 1， 2002 to December 31， 2022 in both Chinese and English， were searched from databases of China National Knowledge Infrastructure （CNKI）， Wanfang Data Knowledge Service Platform （WanFang）， and Chinese Science and Technology Journal （CSTJ）， PubMed， Embase and Web of Science by using key words of psoriasis， psoriases， psoriasis vulgaris， acupuncture， needle， acupoint and moxibustion. Then， Microsoft Excel 2021 was used for data entry， extraction and sorting of the included literature， data extraction mainly includes diagnostic criteria， treatment methods， treatment frequency， total course of treatment， selection of acupoints， efficacy evaluation and outcomes. SPSS Modeler 18.1 software was used to make analysis on the association rules of acupoints.ResultsA total of 26 papers were included， and the diagnostic criteria of clinical studies on acupuncture treatment of psoriasis are constantly updated. Of the 26 clinical studies， 23 had both treatment group and control group， 21 were randomized controlled trials. The main methods for treatment of psoriasis included fire needle， acupuncture， electroacupuncture， acupuncture combined with moxibustion or fire needle， fire needle plus western medicines， etc. with the acupuncture combined with moxibustion， and fire needle combined with western medicines being most frequently used. The observation index and clinical efficacy evaluation were effective rate， psoriasis area and severity index， dermatology life quality index， Traditional Chinese Medicine syndrome score， etc.， with the effective rate being about 40% to 100%. Comparison of clinical efficacy of acupuncture in treating psoriasis showed that the simple fire needle， simple acupuncture， acupuncture combined with moxibustion， fire needle or acupuncture combined with western medicines were superior to simple western medicines， etc. In regard to acupoint selection， a total of 35 acupoints were found in the 26 studies included， among which the top 5 frequently used were Ashi points （skin lesion region）， Geshu （BL17）， Feishu （BL13）， Ganshu （BL18） and Quchi （LI11）， and there were strong association rules among BL17， BL13， BL18 and Shenshu （BL23）.ConclusionAcupuncture is safe and effective in the treatment of psoriasis， and acupuncture combined with moxibustion and fire acupuncture needle combined with western medicines are recommended as the main intervention method， with Ashi-point， BL13， BL17， BL18 and BL23 as the main acupoints.关键词：Psoriasis vulgaris;Acupuncture;Clinical research;Clinical efficacy;Acupoint selection rules100|7|0更新时间：2024-12-05
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