最新刊期
卷 50 ， 期 1 ， 2025
- “针灸科学研究联盟成立，将推动针灸科研、临床转化和装备研发协同创新，为针灸现代化发展提供新动力。”摘要：The establishment of the “Acupuncture Scientific Research Alliance” aims to realize the collaborative innovation of scientific research， clinical transformation and equipment development through multidisciplinary cooperation. In the future， the research will focus on the modern interpretation of acupuncture and moxibustion theory， the systematic analysis of acupoint association atlas and functions， the exploration of systematic biological laws of acupuncture-moxibustion regulation， the quantitative study of key variables of acupuncture-moxibustion effects， the mutual transformation of achievements of clinical research and experimental research， the transformation of basic science research and diagnosis-treatment equipment of acupuncture-moxibustion related external treatment methods， etc.， so as to form a linkage mechanism of talents， platforms and technologies.关键词：Acupuncture and moxibustion;Acupuncture and moxibustion scientific research;Interdisciplinarity cooperation;Linkage mechanism80|10|0更新时间：2025-06-20
- “最新研究发现，电针特定穴位能通过激活大脑特定区域神经元，有效缓解神经病理性疼痛引发的焦虑和抑郁行为。”摘要：ObjectiveTo investigate whether electroacupuncture （EA） at “Yanglingquan” （GV34） and “Xuanzhong” （GB34） can alleviate anxio-depression-like behaviors induced by neuropathic pain and whether the effect of EA is in connection with the activity of glutamatergic neurons in the ventrolateral orbital cortex （VLO）.MethodsThe neuropathic pain model was established by common peroneal nerve ligation （CPNL）. GV34 and GB34 were selected in EA treatment （2 Hz/15 Hz， 1 mA）， 15 min each time， once a day for 7 d. Chemogenetic mixture viruses rAAV-VGLUT1-CRE-WPREs and rAAV-Ef1a-DIO-hM3Dq-mCherry-WPREs or rAAV-Ef1a-DIO-hM4Di-mCherry-WPREs were injected into the VLO， respectively. Clozapine-N-oxide （CNO） was injected intraperitoneally to regulate the activity of glutamatergic neurons in the VLO precisely. Mechanical withdrawal threshold （MWT） and paw withdrawal latency （PWL） were measured by dynamic plantar tactile instruments and plantar thermal nociceptor instruments. Anxio-depression-like behaviors were measured by behavioral tests， including open field test， elevated plus maze test， forced swimming test， and tail suspension test. The activation and inhibition levels of neurons were observed by immunofluorescence staining.ResultsCompared with the sham group， the MWT and PWL of CPNL mice were significantly decreased （P<0.001） on the 3rd day after surgery and lasted until the 28th day. After CPNL， the activity time spent in the central area of open field test reduced （P<0.001）， the percentage of entries and activity time in the open arm of elevated plus maze test were significantly reduced （P<0.001， P<0.05）； the immobile duration of forced swimming test and tail suspension test were remarkably prolonged （P<0.01， P<0.05） of mice in the CPNL group. However， after EA treatment， compared with the CPNL group， the anxio-depression-like behaviors of the mice were alleviated （P<0.05， P<0.01， P<0.001）. Activating glutamatergic neurons of VLO produced an anti-anxio-depression effect （P<0.05， P<0.01） in neuropathic pain mice. Compared with the inhibited+EA+saline group， inhibiting glutamatergic neurons of VLO reversed （P<0.001， P<0.01， P<0.05） the effect of EA.ConclusionEA at GV34 and GB34 can alleviate anxio-depression-like behaviors induced by neuropathic pain through the modulation of glutamatergic neuron activity in the VLO.关键词：Ventrolateral orbital cortex;Neuropathic pain;Glutamatergic neurons;Electroacupuncture;Anxio-depression-like behavior89|12|0更新时间：2025-06-20
- “最新研究发现，电针能有效缓解面神经损伤小鼠焦虑行为，可能通过提高前扣带回皮层谷氨酸能神经元兴奋性实现。”摘要：ObjectiveTo investigate the effect and mechanism of electroacupuncture （EA） at “Siguan” acupoints （bilateral Hegu ［LI4］ and bilateral Taichong ［LR3］） in ameliorating facial nerve injury-induced anxiety-like behavior in mice.MethodsThirty male C57BL/6J mice were randomly divided into control， model （unilateral facial nerve injury）， and EA groups， with 10 mice in each group. The facial nerve injury model was established by lightly clamping the isolated facial nerve for about 30 s， twice altogether after operation. The mice of the EA group were subjected to EA stimulation （2 Hz， 0.5 mA） of bilateral LI4 and bilateral LR3 for 30 min， once per day for consecutive 7 d. The anxiety-like behavior of mice was evaluated by light-dark chamber （LDC） and elevated plus maze （EPM） tests after EA intervention. The calcium activity of glutamatergic neurons in the anterior cingulate cortex （ACC） was analyzed by fiber photometry. The intrinsic excitability level of ACC glutamatergic neurons was estimated by using whole-cell patch clamp recording.ResultsCompared with the control group， the dwell time in the light chamber and the number of entries into the light chamber of LDC tests， and the dwell time in the open arms and the number of entries into the open arms of the EPM tests were significantly decreased in the model group （P<0.01）. In comparison with the model group， the dwell time in the light chamber and the number of entries into the light chamber， and the dwell time in the open arms and the number of entries into the open arms were significantly increased in the EA group （P<0.01，P<0.05）. Results of fiber photometry showed a decreased activity of ACC glutamatergic neurons when the mice moved from the closed arm to the open arm in an EPM， a decrease of input resistance and the number of discharge spikes induced by 300 pA current injection in the model group （P<0.05， P<0.01）. Compared with the model group， the excitability of the glutamatergic neurons， input resistance and the number of discharge spikes （induced by 300 pA current injection） were considerably increased in the EA group （P<0.05）.ConclusionEA of “Siguan” acupoints alleviates the facial nerve injury-induced anxiety-like behavior， probably by decreasing the excitability of glutamatergic neurons in the ACC.关键词：Electroacupuncture;“Siguan” acupoints;Anxiety-like behavior;Facial nerve injury;Anterior cingulate cortex;Glutamatergic neurons92|9|0更新时间：2025-06-20
- “最新研究发现，电针通过激活AMPK信号通路，改善血管性痴呆大鼠认知功能。”摘要：ObjectiveTo observe the effect of electroacupuncture（EA） on adenosine monophosphate-activated protein kinase （AMPK） signaling pathway and mitochondrial energy metabolism in vascular dementia （VD） rats， so as to investigate its potential mechanisms underlying prevention and treatment of VD.MethodsMale SD rats were randomly divided into sham surgery group， model group， EA group， non-acupoint group， and EA+inhibitor group， with 8 rats in each group. Except for the sham surgery group， VD model was established in other 4 groups by permanent bilateral common carotid artery ligation. Rats in the EA group received EA at “Baihui”（GV20） and “Shenting”（GV24）， while rats in the non-acupoint group received EA at the non-acupoint 10 mm above the iliac crest bilaterally. The EA frequency was 2 Hz/15 Hz， duration was 30 min per session， once a day， for 7 d continuously. Rats in the EA+inhibitor group received intraperitoneal injection of AMPK inhibitor Compound C 2 h before EA intervention. After intervention， Morris water maze test was used to evaluate the learning and memory ability of rats. ELISA was used to detect the contents of mitochondrial complexes Ⅰ—Ⅳ in the hippocampus. Colorimetric method was used to detect the content of adenosine triphosphate （ATP） in the hippocampus. Transmission electron microscopy was used to observe the ultrastructure of hippocampal mitochondria. Western blot was used to detect the protein expressions of hippocampal AMPKα1， fatty acid translocase （FAT）/cluster of differentiation 36 （CD36）， and acetyl-CoA carboxylase 2 （ACC2）.ResultsCompared with the sham surgery group， the escape latency and total distance traveled of rats in the model group increased （P<0.01）， while the time spent in the target quadrant and the number of original platform crossings decreased （P<0.01）. The contents of mitochondrial complexes Ⅰ—Ⅳ and ATP in the hippocampus decreased （P<0.01）， mitochondrial structure was severely damaged， and the expressions of hippocampal AMPKα1， FAT/CD36 proteins decreased （P<0.01）， while ACC2 expression increased （P<0.01）. Compared with the model group， the escape latency and total distance traveled of rats in the EA group decreased （P<0.01）， while the time spent in the target quadrant and the number of original platform crossings increased （P<0.01）. The contents of mitochondrial complexes Ⅰ—Ⅳ and ATP in the hippocampus increased （P<0.01）， and the mitochondrial structure showed mild abnormalities. The expressions of hippocampal AMPKα1 and FAT/CD36 proteins increased （P<0.01）， while ACC2 expression decreased （P<0.01）， and the improvement in the EA group was better than that in the non-acupoint group and EA+inhibitor group （P<0.01）. Correlation analysis showed that mitochondrial complex I content was negatively correlated with the escape latency and total distance traveled （P<0.000 1）， and positively correlated with the time spent in the target quadrant and the number of original platform crossings （P<0.05， P<0.000 1）.ConclusionEA can improve cognitive impairment in VD rats by activating AMPK， up-regulating FAT/CD36 expression， and down-regulating ACC2 expression， thereby promoting mitochondrial fatty acid metabolism and improving mitochondrial morphology and function.关键词：Vascular dementia;Electroacupuncture;Mitochondria;Energy metabolism;Adenosine monophosphate-activated protein kinase82|10|0更新时间：2025-06-20
- “最新研究发现，毫针、电针和艾灸能有效改善膝关节骨关节炎症状，调节肌肉萎缩相关蛋白，三种方法疗效相当。”摘要：ObjectiveTo compare the effects of filiform acupuncture， electroacupuncture （EA） and moxibustion on gastrocnemius inflammation and muscle atrophy related proteins in rats with knee osteoarthritis （KOA）.MethodsA total of 40 Wistar rats were randomly divided into blank control， model， filiform acupuncture， EA and moxibustion groups， with 8 rats in each group. The KOA model was established by intra-articular injection of sodium iodate into the right knee joint in the model group and treatment groups （filiform acupuncture， EA and moxibustion groups）. Treatment groups received filiform acupuncture， EA and moxibustion on the right “Futu” （ST32） and “Housanli”（ST36） for 15 min each time， once every other day， for 4 weeks. The diameter of the right knee joint of rats in each group were measured and the structure of knee joint was observed by X-ray before modeling， after modeling， and after treatment. After treatment， the Pinnate angle， muscle thickness and cross-sectional area of right rectus femoris were measured by ultrasound， the ultrastructure of right gastrocnemius muscle was observed by transmission electron microscope， the contents of tumor necrosis factor-α （TNF-α）， interleukin （IL）-6 and IL-10 in right gastrocnemius muscle were detected by enzyme-linked immunosorbent assay （ELISA）， and the expression levels of sirtuin1（SIRT1），muscle-specific ringfinger protein 1（MuRF1），nuclear factor erythroid 2-related factor 2（NRF2） and recombinant transcription factor A（TFAM） in right gastrocnemius muscle were detected by Western blot in each group.ResultsCompared with the blank control group， the diameter of the right knee joint increased （P<0.01）， the knee joint space of the model group was obviously narrowed， accompanied by osteophyte formation at the joint edge and subchondral bone reactive hyperplasia， the Pinnate angle， muscle thickness and cross-sectional area of rectus femoris decreased （P<0.01）， the mitochondria of gastrocnemius muscle cells swelled， the muscle fibers arranged disorderly， the sarcomere structures were destroyed， the Z-line fractures were discontinuous， the contents of TNF-α and IL-6 in gastrocnemius muscle were increased （P<0.01）， and IL-10 was decreased （P<0.01）， the protein expression of MuRF1 was increased （P<0.01）， whereas the expressions of SIRT1， NRF2， and TFAM proteins decreased （P<0.01） in the model group. Compared with the model group， all these indicators were reversed in the treatment groups （P<0.01， P<0.05）. In the moxibustion group， the muscle thickness of rectus femoris was higher （P<0.01） than those in the filiform acupuncture and EA groups， the Pinnate angle was higher （P<0.01） than that in the EA group， and the cross-sectional area was lower （P<0.05） than that in the EA group. The content of IL-10 in gastrocnemius muscle in the EA group was higher （P<0.01） than those in the moxibustion and filiform acupuncture groups. The expressions of MuRF1 protein in the gastrocnemius muscle were lower （P<0.01） in the EA and moxibustion groups compared to the filiform acupuncture group.ConclusionFiliform acupuncture， EA and moxibustion can improve knee joint swelling， ameliorate muscle ultrastructure and muscle fiber， regulate inflammatory cytokine level， improve KOA by modulating muscle atrophy related proteins SIRT1/MuRF1 and NRF2/TFAM. There was no significant difference in the efficacy among the three methods of acupuncture and moxibustion.关键词：Knee osteoarthritis;Filiform acupuncture;Electroacupuncture;Moxibustion;Muscle inflammation;Muscle atrophy59|3|0更新时间：2025-06-20
- “最新研究发现，电针“公孙”能恢复POI大鼠体重，调节性激素，提高抗氧化能力，减轻卵巢损伤，促进卵泡发育，可能通过调节Keap1/Nrf2/HO-1通路发挥作用。”摘要：ObjectiveBased on the theory of “Gongsun （SP4） connects the Thoroughfare Vessel” of the “eight confluence acupoints”， the effect of electroacupuncture （EA） at SP4 on Kelch-like epichlorohydrin associated protein factor 1 （Keap1）/ nuclear factor-erythroid 2 related factor 2 （Nrf2）/ heme oxygenase-1 （HO-1） signaling pathway was observed in rats with premature ovarian insufficiency （POI） for exploring its underlying mechanisms in treating POI.MethodsThirty-two female SD rats were randomly divided into 4 groups： blank， model， EA and medication， with 8 rats in each group. The POI model was established by intraperitoneal injection of cyclophosphamide. EA （2 Hz， a strength inducing slight limb tremor） was applied to bilateral SP4 for 20 min， once a day for a total of 14 d. Rats of the medication group were treated by gavage of solution of estradiol valerate tablets （0.09 mg/kg） once a day for 14 d. During the experiment， the changes of body mass in each group were recorded and the general conditions were observed. After the interventions， the contents of follicle-stimulating hormone （FSH）， estradiol （E2）， anti-mullerian hormone （AMH）， malondialdehyde （MDA） and the activity of superoxide dismutase （SOD）， glutathione peroxidase （GSH-Px） were detected by ELISA. The morphological changes of ovarian tissue were observed under light microscope after H.E. staining. The protein and mRNA expression levels of Keap1， Nrf2 and HO-1 in the ovarian were detected by immunohistochemistry， Western blot， and PCR， respectively.ResultsAfter modeling， the model rats had poor mental state， reduced activity， irritability， emaciated body， dry and dull hair， decreased food intake， and occasionally yellow soft stools， black stools， and bloody secretions around the eyes and the tip of the nose. After the interventions， the mental state of rats in the EA and medication groups was recovered， and had brown oval feces， with no bloody secretion. Compared with the blank group， the body mass， levels of serum E2， AMH， SOD， GSH-Px， immunoactivity of Nrf2 and HO-1， and expression levels of Nrf2 and HO-1 proteins and mRNAs in the ovarian tissue were significantly decreased （P<0.01）， and the contents of serum FSH and MDA， immunoactivities and expressions of Keap1 protein and mRNA were obviously increased （P<0.01） in the model group. Following the interventions， both the decrease in the levels of E2， AMH， SOD， GSH-Px， and the protein and mRNA expression levels of Nrf2 and HO-1 and the increase of contents of serum FSH and MDA， immunoactivities and expressions of Keap1 protein and mRNA were reversed in both EA and medication groups （P<0.01， P<0.05）， without significant differences between the two groups in all the indexes mentioned above. H.E. staining showed a decrease in the primordial and growing follicles in the ovarian cortex and an increase in the atretic follicles and disordered and loose arrangement of the granular layer in the model group， and an increase in the primordial and growing follicles in the ovarian cortex， reduction in the atretics follicles， thickened granular layer and closer arrangement in the EA and medication groups.ConclusionEA of SP4 can promote the restoration of the body mass， regulate the level of serum sex hormones， improve the body’s antioxidant capacity， reduce the damage of oxidative stress of ovaries and promote the normal development of follicles in POI rats， which may be related to its function in regulating Keap1/Nrf2/HO-1 signaling pathway.关键词：Premature ovarian insufficiency;Electroacupuncture;Gongsun （SP4）;Oxidative stress;Keap1/Nrf2/HO-1 signaling pathway67|9|0更新时间：2025-06-20
- “最新研究发现，针刺能改善卵巢储备功能减退大鼠的卵巢功能，可能通过抑制氧死亡信号通路发挥作用。”摘要：ObjectiveTo observe the effect of acupuncture on the oxeiptosis Kelch-like ECH-associated protein 1 （KEAP1）/phosphoglycerate mutase 5 （PGAM5）/mitochondrial apoptosis-inducing factor 1 （AIFM1） signaling pathway in rats with diminished ovarian reserve （DOR）， so as to explore the possible mechanisms by which acupuncture improves ovarian reserve function in DOR rats.MethodsFemale SD rats were randomly divided into the control group， model group， and acupuncture group， with 6 rats in each group. The DOR model was established by one-time intraperitoneal injection of 75 mg/kg cyclophosphamide. After modeling， the acupuncture group received acupuncture at “Guanyuan” （CV4）， “Zhongji” （CV3）， “Qihai” （CV6）， and bilateral “Sanyinjiao” （SP6）， “Zigong” （EX-CA1） acupoints for 20 min each time， every other day， for a total of 15 sessions. During the experiment， the estrous cycles of the rats were observed and recorded daily. After the intervention， the body mass and ovarian mass were measured， and the ovarian index was calculated. Serum contents of estradiol （E2）， follicle-stimulating hormone （FSH）， luteinizing hormone （LH）， and anti-Müllerian hormone （AMH） were detected by ELISA. HE staining was used to observe ovarian tissue morphology， follicle development， and ovulation. The contents of local reactive oxygen species （ROS） in ovarian tissue were measured by flow cytometry， and the protein expression levels of KEAP1， PGAM5， and AIFM1 were detected by Western blot.ResultsCompared with the control group， the model group exhibited irregular estrous cycles， increased contents of FSH and LH （P<0.05）， and decreased contents of E2 and AMH （P<0.05）. There was a reduction in the number of follicles at all stages and an increase in atretic follicles， with significantly elevated protein expression levels of PGAM5 and AIFM1 in ovarian tissue （P<0.05）. After acupuncture intervention， compared to the model group， the acupuncture group showed slight improvement in the disruption of the estrous cycle， decreased contents of FSH and LH （P<0.05）， increased contents of E2 and AMH （P<0.05）， an increase in the number of follicles at all stages， and a decrease in the expression level of PGAM5 in ovarian tissue （P<0.05）. There was no significant difference in ovanian index and ROS content in ovarian tissue among the 3 groups.ConclusionAcupuncture can improve ovarian reserve function， and its mechanism may be related to the inhibition of the oxeiptosis pathway.关键词：Diminished ovarian reserve;Acupuncture;Reactive oxygen species;oxidative cell death pathway63|5|0更新时间：2025-06-20
- “最新研究发现，电针能改善SAMP8小鼠焦虑行为和记忆能力，可能通过上调海马髓鞘蛋白表达促进髓鞘再生。”摘要：ObjectiveTo observe the effects of electroacupuncture （EA） on anxiety-like behavior， learning and memory ability and expressions of myelin-related proteins in the hippocampus of senescence accelerated mice prone 8 （SAMP8）， so as to explore the mechanism of EA in improving Alzheimer’s disease （AD） from the perspective of myelin sheath injury and regeneration.MethodsTwenty-four male SAMP8 mice were randomly and equally divided into model group and EA group. Twelve male SAMR1 mice of the same age as SAMP8 mice were used as the control group. The rats in the EA group received acupuncture at “Baihui” （GV20）， “Dazhui” （GV14） and “Shenshu” （BL23）， among which GV14 and BL23 received EA intervention （1.5—2 mA， 2 Hz）， the intervention lasted for 20 min once daily for a total of 3 courses， with every 8 d as a course and an interval of 2 d between each 2 courses. The anxiety-like behavior of mice was observed by open field test. The learning and memory ability of mice was observed by Morris water maze test. The neuronal demyelination in the hippocampus of mice was observed after luxol fast blue（LFB） staining. The positive expressions of myelin basic protein （MBP） and myelin protein lipid protein （PLP） in hippocampal CA1 region were detected by immunofluorescence. The mRNA expression levels of MBP， chondroitin sulfate proteoglycan （NG2） and PLP， and the protein expression levels of myelin-associated proteins MBP， NG2， PLP， myelin-associated glycoprotein （MAG） and myelin oligodendrocyte glycoprotein （MOG） in the hippocampus were detected by qPCR and Western blot， respevtively.ResultsCompared with the control group， the activity distance and activity time in the central region in open field test of the model group were significantly shortened （P<0.01）， the escape latency of the Morris water maze test was prolonged （P<0.01）， the number of crossing the original platform and the residence time in the original platform quadrant were decreased （P<0.01）. The LFB staining in the hippocampus was shallow， the myelin fibers were sparse， the positive expressions of MBP and PLP in the hippocampal CA1 region， and the mRNA expression levels of MBP， NG2 and PLP， as well as the protein expression levels of MBP， NG2， PLP， MAG and MOG in the hippocampus were significantly decreased （P<0.01） in the model group than those of the control group. After the acupuncture or EA interventions， the activity distance and activity time in the central region in open field test of the mice in the EA group were significantly prolonged （P<0.01） ， the escape latency of the Morris water maze test was shortened （P<0.01） ， the number of crossing the original platform and the residence time in the original platform quadrant were increased （P<0.01） ， the LFB staining in the hippocampus was deepened， the myelin fibers were arranged more densely， the positive expression levels of MBP and PLP in the hippocampal CA1 region， and the mRNA expression levels of MBP， NG2 and PLP in the hippocampus， and the protein expression levels of MBP， NG2， PLP， MAG and MOG in the hippocampus were all increased （P<0.01，P<0.05） in the EA group relative to the model group.ConclusionEA can improve the anxiety-like behavior and learning and memory ability of SAMP8 mice， which may be related to its effects in improving myelin regeneration via up-regulating myelin-related proteins expressions in the hippocampus.关键词：Alzheimer’s disease;Electroacupuncture;Myelin sheath;Learning and memory ability;SAMP8 mice82|7|1更新时间：2025-06-20
- “最新研究发现，热补针法能调节类风湿性关节炎滑膜组织中长链非编码RNA表达，抑制滑膜炎性反应，改善症状。”摘要：ObjectiveTo observe the effect of heat-reinforcing needling on the expression profile of long non-coding RNA （LncRNA） in the synovial tissue of rabbits with cold-syndrome type rheumatoid arthritis （RA）， so as to explore its mechanisms underlying improvement of RA.MethodsA total of 18 New Zealand rabbits were randomly divided into normal， model and heat-reinforcing needling （acupuncture） groups， with 6 rabbits in each group. The RA model was established by injection of mixed solution （emulsifier） of ovalbumin powder（80 mg） + NaCl （20 mL） +Freund’s complete adjuvant （20 mL）， combined with cold compress （ice cube +crystal calcium chloride） of the knee-joint. In addition， rabbits of the normal and model groups received regular grasping and fixation in the same way as those of the acupuncture group. For rabbits of the acupuncture group， the heat-reinforcing needling stimulation was applied to bilateral “Zusanli” （ST36） for about 1 min， followed by retaining the needle for 30 min， once a day， for a total of 7 d. The rabbits’ general condition was observed， and the knee joint circumference and pain threshold after intervention were measured. The knee-joint cavity effusion， synovial thickness， and internal blood flow signals were observed using a color Doppler ultrasound apparatus. Histopathological changes of synovium were observed using H.E. staining. The contents of serum tumor necrosis factor α （TNF-α）， interleukin （IL）-1β and IL-6 were detected using enzyme linked immunosorbent assay （ELISA）， and the differential LncRNAs and biological functions in the synovial tissues were detected and analyzed using high-throughput sequencing technology. The expression levels of 5 significantly differentially expressed LncRNAs were verified using qPCR.ResultsCompared with the normal group， the model group of rabbits showed a significant increase in the knee joint circumference （P<0.05） and the content of serum TNF-α， IL-1β， IL-6 （P<0.05）， and a significant decrease in the pain threshold （P<0.05）， and diffuse synovial hyperplasia of the synovial membrane， joint effusion and abnormal blood flow signals， proliferated and thickened synovial lining， with visible capillary formation beneath the lining and a large number of inflammatory cell clustering. In comparison with the model group， the knee joint circumference， and the serum contents of TNF-α， IL-1β and IL-6 were obviously decreased （P<0.05）， while the pain threshold was notably increased （P<0.05）， and H.E. staining showed a reduction in the hyperplasia of synovial lining， with a milder inflammatory cell infiltration. High-throughput sequencing and bioinformatics analysis displayed that a total of 142 LncRNAs were found to have opposite expression trends between the acupuncture group and the model group， of which 83 LncRNAs were up-regulated in the model group vs the normal group and down-regulated in the acupuncture group vs the model group， mainly involving the core functional clusters of cell cycle， mitogen-activated protein kinase （MAPK） signaling and cell senescence， and 59 LncRNAs were down-regulated in the model group vs the normal group， and up-regulated in the acupuncture group vs the model group， mainly involving the metabolic pathway， peroxisome proliferator-activated receptor （PPAR） signaling and AMP-dependent protein kinase （AMPK） signaling. The results of qPCR showed that compared with the normal group， the expression levels of MSTRG.15448.1 and MSTRG.17571.1 in the synovial tissue of knee joint were significantly decreased （P<0.05）， and those of MSTRG.5134.1， MSTRG.13294.1 and MSTRG.15972.1 were significantly increased （P<0.05） in the model group. Compared with the model group， the expression levels of MSTRG.15448.1 and MSTRG.17571.1 were significantly increased （P<0.05）， and those of MSTRG.5134.1， MSTRG.13294.1 and MSTRG.15972.1 were significantly decreased （P<0.05） in the acupuncture group.ConclusionThe heat-reinforcing needling can regulate the expression profile of LncRNAs in the RA synovial tissue， which may be one of the mechanisms underlying inhibition of synovial inflammatory response and improvement of symptoms.关键词：Rheumatoid arthritis;heat-reinforcing needling;Long noncoding RNA58|4|0更新时间：2025-06-20
- “最新研究发现，针刺能改善功能性消化不良伴抑郁样行为大鼠的胃肠动力障碍和突触损伤，可能通过调节脑肠肽水平和5-HT系统发挥作用。”摘要：ObjectiveTo observe the effect of acupuncture on gastrointestinal motility， synaptic structure， brain-gut peptide and 5-hydroxytryptamine （5-HT） system in rats with functional dyspepsia （FD） and depression， so as to explore its mechanism underlying improvement of FD and depression-like behavior.MethodsForty male SD rats were randomly divided into control， model， acupuncture and medication （fluoxetine） groups with 10 rats in each group. The model of FD with depression comorbidity was established by multi-factor stress conditioning （chronic unpredictability stimulation + modified tail-clamp irritation stimulation+intermittent fasting） for 28 d. Acupuncture was applied to “Baihui” （GV20）， bilateral “Zusanli”（ST36） and “Zhongwan” （CV12） for 20 min， once daily for 7 d. The rats in the medication group received intraperitoneal injection of fluoxetine （20 mg/kg） once daily for 7 d. The suppression-like behavior was assessed by using tail suspension immobility time in 4 min， forced swimming immobility time in 4 min and sucrose preference rate. The gastrointestinal motility was assessed by using gastric emptying rate and small intestinal propulsion rate. The ultrastructural changes of myelin and synapse in the hippocampus were observed by transmission electron microscope. ELISA was used to detect the contents of serum somatostatin， motilin and 5-HT， and Western blot was used to detect the protein expression levels of tryptophan hydroxylase 1 （TPH1） and 5-hydroxytryptamine receptor 3 （5-HT3R） in hippocampus.ResultsCompared with the control group， the tail suspension immobility time and forced swimming immobility time were significantly increased （P<0.05）， the sucrose preference rate， gastric emptying rate and small intestinal propulsion rate， contents of serum somatostatin， motilin and 5-HT， and the protein expression levels of TPH1 and 5-HT3R in the hippocampus were significantly decreased （P<0.05） in the model group. In comparison with the model group， the tail suspension immobility time and forced swimming immobility time were significantly decreased （P<0.05）， whereas the sucrose preference rate， gastric emptying rate and small intestinal propulsion rate， contents of serum somatostatin， motilin and 5-HT， and the protein expression levels of TPH1 and 5-HT3R in the hippocampus were significantly increased （P<0.05） in the acupuncture group. Compared with the model group， the changes in behavioral indexes， number of synapses， serum 5-HT content， hippocampal TPH1 and 5-HT3R protein expression levels in the medication group were same as those in the acupuncture group （P<0.05）. Outcomes of transmission electron microscopy displayed that in the model group， the hippocampus had obvious myelin degeneration and lysis， fewer microfilaments and microtubules， blurred mitochondria with loss of a large number of organelles， which was relatively milder in both acupuncture and medication groups. Both acupuncture and medication interventions evidently reversed modeling-induced reduction in the number of hippocampal synapses （P<0.05）.ConclusionAcupuncture can improve the gastrointestinal motility and hippocampal synaptic damage in rats with FD and depression， which may be related to its functions in up-regulating the levels of brain-gut peptides and 5-HT system.关键词：Functional dyspepsia;Depression-like behavior;Acupuncture;5-HT system;Comorbidity;Synapse86|8|0更新时间：2025-06-20
- “电针疗法可改善小鼠肾脏纤维化，延缓肾脏老化，抑制PI3K/Akt炎性通路可能是其机制之一。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Baihui”（GV20） and“Shenshu”（BL23） on the morphological changes of aging kidney and phosphatidylinositol 3-kinase /protein kinases B （PI3K/Akt） signaling in SAMP8 mice， so as to explore its mechanisms of delaying renal aging.MethodsA total of 20 seven-month-old male SAMP8 mice were randomly divided into model group and EA group， with 10 mice in each group. The normal group consisted of 10 male SAMR1 mice with the same age. In the EA group， acupuncture was performed at GV20 and bilateral BL23 respectively. EA （2 Hz， 1 mA） was applied to bilateral BL23 for 15 min every time， once a day， 10 days as a course for a total of 4 courses， the interval between courses was one day. The general condition of the mice in each group was observed during the intervention period. After the intervention， H.E. staining was used to observe the morphological changes of aging kidney. Masson staining was used to evaluate the degree of renal fibrosis. The relative protein expression levels of alpha smooth musle action （α-SMA）， interleukin-1β （IL-1β）， interleukin-4 （IL-4）， PI3K and Akt in renal tissue were detected by Western blot.ResultsCompared with the normal group， mice in the model group showed dull hair， reduced daily activity and food and water intake， and poor response to external stimulation， and the relative protein expression levels of α-SMA， IL-1β， PI3K and Akt in renal tissue were significantly increased （P<0.001， P<0.05， P<0.01）， while the protein expression level of IL-4 was significantly decreased （P<0.01）. After EA intervention， compared with the model group， the general condition of rats in the EA group was significantly improved， and the relative protein expression levels of α-SMA， IL-1β， PI3K and Akt were obviously decreased （P<0.05）， while the level of IL-4 was significantly increased （P<0.05）. H.E. staining and Masson staining showed that the glomeruli of the mice in the model group were atrophied and sclerotic， the renal tubules were hypertrophied and disorganized， the inflammatory cell infiltration was obvious， and the collagen fibers of the renal interstitium were obviously increased， which was relatively milder in the EA group.Conclusion“Kidney-reinforcing and Govenor Vessel-regulating” EA can inhibit the over-expressions of IL-1β and α-SMA and increase the expression of IL-4 in aging renal tissue， which may contribute to its effects in delaying renal aging. Inhibiting the PI3K/Akt signaling may be one of the possible mechanisms of EA anti-renal fibrosis.关键词：Electroacupuncture;Aging;Phosphatidylinositide 3-kinase/protein kinase B signaling;Renal fibrosis51|4|0更新时间：2025-06-20
- “在动眼神经麻痹治疗领域，专家验证了标本根结理论指导下的针刺治疗，有效改善患者症状，疗效优于常规针刺。”摘要：ObjectiveTo observe the clinical effect of acupuncture in treatment of oculomotor nerve palsy guided by Biaoben Genjie theory （focusing on the distribution， connection and gathering of meridian qi in the body）.MethodsA total of 62 patients with oculomotor nerve palsy were selected according to inclusion criteria of the trial. They were randomly divided into a control group and an observation group， with 31 cases in each group. The conventional treatment with Western medicine was used in the two groups. Besides， in the control group， the regular acupuncture was provided， and in the observation group， acupuncture was delivered under the guidance of Biaoben Genjie theory. In the 2 groups， the treatment was given once daily， for 30 min each time and one course of treatment was composed of 6 d. Six courses were required with the interval of 1 d between two courses. The degree of diplopia， the degree of strabismus and the score of traditional Chinese medicine （TCM） main symptoms were recorded before and after the treatment of the 2 groups separately， and the clinical efficacy was evaluated in each group.ResultsThe degrees of diplopia and strabismus decreased significantly （P<0.05） compared with those before treatment in the 2 groups， and the degrees in the observation group were lower （P<0.05） than those of the control group. The TCM main symptoms score of both groups were reduced significantly （P<0.05） compared with those before treatment， and the score in the observation group was lower （P<0.05） than that of the control group. The effective rate was 93.55% （29/31） in the observation group， better （P<0.05） than that （83.87%， 26/31） of the control group.ConclusionAcupuncture based on Biaoben Genjie theory can effectively relieve the ocular symptoms of the affected eyes in the patients with oculomotor nerve palsy， and the effect is better when compared with the regular acupuncture.关键词：Oculomotor nerve palsy;Biaoben Genjie theory;Acupuncture;Clinical observation44|6|0更新时间：2025-06-20
- “针灸治疗脊髓损伤新机制：调节生长因子、抑制瘢痕形成、激发轴突生长能力。”摘要：Based on the growth factor theory， external inhibition theory， and neuronal intrinsic theory， we， in this paper， summed up the potential mechanisms of acupuncture in the treatment of spinal cord injury （SCI） by promoting the axonal regeneration in recent years. Acupuncture can promote the repairment of SCI by （1） regulating the expressions of spinal cord growth factors （such as nerve growth factor， brain derived neurotrophic factor， neurotrophin-3， glial cell line-derived neurotrophic factor， ciliary neurotrophic factor， insulin-like growth factor， etc.） to support neuronal survival and axon growth； （2） inhibiting the expression of spinal cord myelin growth inhibitor Nogo protein and oligodendrocyte myelin glycoprotein （OMgp）， requlating axon guide molecules Sema3A and Wnt family， etc.， and inhibiting the formation of astryocyte scar to create a favorable local microenvironment for axon regeneration； and （3） regulating spinal phosphatase and tensin homolog deleted on chromosome ten （PTEN）/mammalian target of rapamycin （mTOR） signaling， down-regulating Ras homolog gene family member A （RhoA） / Rho-associated protein kinase Ⅱ （ROCKⅡ） signaling， up-regulating mitogen-activated protein kinase kinase （MAPK）/ extracellular signal-regulated kinase （ERK） signaling， cAMP/ protein kinase A （PKA） signaling， etc.. In addition， we also found that by modulating axon regeneration， acupuncture therapy can not only effectively restore motor function in SCI patients， but also improve neurogenic bladder symptoms.关键词：Spinal cord injury;Acupuncture;Axon regeneration;Mechanism;Review95|5|0更新时间：2025-06-20
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Research progress on the rules of meridian phenomenon and the mechanism in gastrointestinal diseases AI导读
“最新研究发现，胃肠道疾病可引发体表特异性表征现象，揭示经络与脏腑疾病联系的生物学机制。”摘要：The meridian theory refers to the understanding of the physiological functions of the human body and the pathological changes of zangfu organs， as well as the different specific represenatation phenomena under the pathological conditions and their interrelationship with the body surface representation and the meridian points. It is of great scientific significance to explore the development of acupuncture based on the meridian theory. This article summarizes the different specific representation phenomena of gastrointestinal diseases on the body surface and the rules. It proposes that the inflammatory responses in zangfu organs may trigger the sensory regeneration and remodeling. These signals travel from the dorsal root ganglia to the brain and spinal cord， and then affect the local immunity， blood vessel function and other aspects at acupuncture locations through sympathetic outflow. It is anticipated that the above summary may provide the further insights for the interconnections between the meridian system and zangfu diseases， the regulatory pathways， and the biological mechanisms .关键词：Gastrointestinal disorders;Meridian phenomena;Acupoint sensitization;Sensory afferents;Nerve conduction47|5|0更新时间：2025-06-20 - “最新研究发现，针刺能有效调节帕金森病患者肠道菌群，延缓病情发展。”摘要：Parkinson’s disease （PD） is a neuro-degenerative disease that is common in middle-aged and elderly people， and its incidence is increasing year by year. Studies have shown that pathological products produced by a series of pathological cascade reactions in the enteric nervous system （ENS） of PD patients travel to the brain through the vagus nerve pathway in the microbial-gut-brain axis， and ultimately lead to the occurrence and development of PD. Acupuncture plays an important role in interfering with pathological products of ENS， restoring ecological balance of intestine and delaying PD process. In this paper， the effects of acupuncture on intestinal flora， production of related microbial products， α-synuclein， neuroinflammatory response and oxidative stress in patients or animal model with PD were reviewed.关键词：Acupuncture;Parkinson’s disease;Enteric nervous system;Microbial-gut-brain axis;Review92|7|0更新时间：2025-06-20
- “针刺镇痛中枢机制研究取得新进展，工具病毒及光/化学遗传学技术为揭示针刺镇痛脑环路机制提供了新的实验手段和突破口。”摘要：The clinical efficacy of acupuncture analgesia is definite， but its mechanisms of the central nervous system have not yet been fully elucidated. The virus vector technique provides an effective means for the study of the central nervous system mechanism of acupuncture analgesia. We， in this article， briefly introduced the classification and characteristics of virus vectors， reviewed and analyzed their application and their functional components carried by and optical/chemical genetics technologies they carry in pain and acupuncture analgesia research in recent years. At present， in the research on the central mechanism of acupuncture analgesia， the viral vectors combined with chemical genetics technology is mainly used to explore the neural circuit mechanisms such as rostral anterior cingulate cortex （rACC）→ventrolateral periaqueductal gray （rACC→vlPAG）， rACC→thalamus （rACC→Th） and ventrolateral periaqueductal gray→hippocampus （vlPAG→HPC）. The viral vectors combined with optogenetics technology is mainly used to explore the neural circuits from the prefrontal infralimbic cortex to the nucleus accumbens （IL→NAc）. The viral vectors and optical/chemical genetics technology can not only target and accurately mark the specific neurons or neural circuits， but also dynamically and real-time monitor the function of neural networks， bringing new experimental means and breakthroughs for the research of acupuncture analgesia mechanism of the brain circuits.关键词：Virus vector tool;Acupuncture analgesia;Optogenetics;Chemical genetics;Brain circuits72|9|0更新时间：2025-06-20
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