最新刊期
卷 50 ， 期 10 ， 2025
- “最新研究发现，艾灸“关元”和“中极”可改善脊髓损伤尿潴留大鼠膀胱纤维化，抑制氧化应激和铁死亡，为治疗提供新方案。”摘要：ObjectiveTo observe the effect of moxibustion on levels of oxidative stress- and ferroptosis-related proteins in the bladder tissues， and bladder fibrosis in rats with spinal cord injury （SCI）-induced urinary retention， so as to explore its mechanisms underlying improvement of bladder function.MethodsFemale Wistar rats were randomly divided into normal control， sham operation， SCI model and moxibustion groups， with 12 rats in each group. The modified Allen’s vertical percussion method was used to establish the model of SCI-induced urinary retention. The rats of the moxibustion group received moxibustion stimulation at “Guanyuan” （CV4） and “Zhongji”（CV3） for 20 min， once a day for 14 days. The bladder function was detected by using a urodynamic analyzer， and the motor function was detected by Basso-Beattie-Bresnahan （BBB） rating scale. After 14 days of intervention， the bladder tissue was collected for observing morphological changes by using HE staining， the interfascicular collagen deposition by using Masson staining， the mitochondrial structure by using electron microscopy， and for detecting the expression levels of nuclear factor E2-related factor 2 （Nrf2）， heme oxygenase-1 （HO-1）， glutathione peroxidase 4 （GPX4）， collagen Ⅰ and collagen Ⅲ by Western blot. The contents of interleukin-1β （IL-1β） and reactive oxygen species （ROS） in serum were detected by ELISA. The contents of ferrous ion （Fe2+） in serum and glutathione （GSH） and malondialdehyde （MDA） in the bladder tissue were detected by colorimetric method.ResultsNo significant differences were found between the normal control and sham operation groups in all the indexes. Compared with the sham operation group， the BBB score， GSH content and the expression levels of Nrf2， HO-1 and GPX4 proteins in the bladder tissue were significantly decreased （P<0.01）， while the bladder capacity， urinary leakage point pressure and bladder compliance， extracellular matrix （ECM） deposition and expression of collagen Ⅰ and Ⅲ in the bladder tissue， and the contents of Fe2+， ROS， IL-1β in serum and MDA in the bladder tissue were significantly increased （P<0.01） in the model group. In comparison with the model group， both the increase of the bladder capacity， urinary leakage point pressure and bladder compliance， ECM deposition and expression of collagen Ⅰ and Ⅲ， and the contents of Fe2+， ROS and IL-1β， and the decrease of the BBB score， GSH content and the expression levels of Nrf2， HO-1 and GPX4 proteins after modeling were reversed in the moxibustion group （P<0.01）. Morphological observations （HE and Masson staining and electron microscope） showed disordered arrangement of muscle fibers and muscle bundles， enlargement of muscle fascicle space， atrophy， severe deposition of ECM， increase of collagen fibers， ferroptosis mitochondrial atrophy， reduction or even disappearance of mitochondrial crests， and increase of membrane density in the bladder tissue of the model group， which was relatively lighter in the injury severity in the moxibustion group.ConclusionMoxibustion stimulation of CV3 and CV4 can improve functional activities of the bladder in SCI-induced urinary retention rats， which may be related to its functions in inhibiting the occurrence of oxidative stress and ferroptosis， and delaying the progression of bladder fibrosis.关键词：Spinal cord injury;Urinary retention;Oxidative stress;Ferroptosis;Bladder fibrosis117|55|0更新时间：2025-11-04
- “最新研究发现，电针能显著改善膝关节骨关节炎症状，可能通过调节肠道菌群和抑制炎症信号通路发挥作用。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on knee function and symptoms， intestinal flora and TLR4/MyD88/NF-κB signaling pathway of articular cartilage in rabbits with knee osteoarthritis （KOA）， so as to explore its potential mechanism underlying improvement of knee function by regulating the gut-joint axis response of KOA.MethodsThirty-two Japanese white rabbits were randomly divided into blank， model， glucosamine and EA groups， with 8 rabbits in each group. The KOA model was reproduced by using modified Hulth method. The rabbits in the EA group were given EA （2 Hz/100 Hz， 0.5—1.5 mA） at bilateral “Xuehai”（SP10）， “Yinlingquan”（SP9） and “Dubi”（ST35） for 15 min， once daily， 5 times a week for 4 weeks. The rabbits of the glucosamine group received glucosamine solution once daily， 5 times a week for 4 weeks. The knee function was evaluated by Lequesne MG index of severity for osteoarthritis （total scores of pain， swelling， range of motion， and gait） after modeling and treatment. H.E. staining was used to observe the histopathological changes of the knee cartilage tissue. Immunofluorescence histochemical method was used to detect the expressions of MyD88 and NF-κB p65， and Wes automated protein blot quantitative analysis technique was employed to detect the protein expressions of TLR4， MyD88 and NF-κB p65 in the knee cartilage tissue. Analysis of the species structure changes of intestinal flora was detected by 16S rDNA high-throughput sequencing.ResultsAfter modeling， compared with the blank group， the Lequesne MG score， immunofluorescence intensity of MyD88 and NF-κB p65， and the protein expression levels of TLR4， MyD88 and NF-κB p65 were significantly increased in the model group （P<0.05）. H.E. staining showed thin and rough surface of the knee cartilage， clusters and disordered arrangement of chondrocytes， a few cellular nuclei being dark blue-purple in staining， with some nuclei being shrank， ruptured， and disappeared in the model group. In comparison with the model group， the Lequesne MG score， fluorescence intensity of MyD88 and NF-κB p65， and the protein expression levels of TLR4， MyD88 and NF-κB p65 were significantly decreased in both EA and glucosamine groups （P<0.05）. The effects of EA were significantly superior to glucosamine in down-regulating the protein expression levels of TLR4， MyD88 and NF-κB （P<0.05）. H.E. staining showed relatively complete surface of the knee cartilage， slightly hypertrophic chondrocytes with relatively clear nucleus and relatively complete tide line in both EA and glucosamine groups. Results of 16S rDNA sequencing showed that compared with the blank group， the Shannon index was obviously increased （P<0.05）， the relative abundance of Proteobacteria， Actinobacteria， Escherichia coli-Shigella and Clostridium UCG-014 was elevated， and the relative abundance of Firmicutes， Verrucomicrobia， Comamonas and Acmea bacteria was decreased in the model group. In comparison with the model group， the Chao1 index and Shannon index were increased （P<0.05）， the relative abundance of Proteobacteria， Actinobacteria and Escherichia coli-Shigella was decreased， while the relative abundance of Firmicutes， Comamonas and Nobilus was significantly increased in both EA and glucosamine groups.ConclusionEA can significantly improve the symptoms and function of knee joint in KOA rabbits， which may be associated with its functions in inhibiting cartilage injury and inflammation reaction by regulating TLR4/MyD88/NF-κB signaling pathway and intestinal flora diversity through the “gut-joint” axis.关键词：Knee osteoarthritis;Electroacupuncture;TLR4/MyD88/NF- κB signaling pathway;Inflammation;Intestinal flora86|25|0更新时间：2025-11-04
- “最新研究发现，热补针法能有效改善寒湿证类风湿关节炎家兔症状，提高滑膜细胞线粒体质量，可能通过抑制Drp1/Mff通路活性发挥作用。”摘要：ObjectiveTo observe the efficacy of heat-reinforcing acupuncture at “Zusanli” （ST36） in rabbits with cold-dampness rheumatoid arthritis （RA） and its impact on mitochondrial quality and the dynamin-related protein 1 （Drp1）/mitochondrial fission factor （Mff） pathway in knee synovial tissue.MethodsTwenty-four New Zealand white rabbits were randomly divided into a normal group， a model group， a heat-reinforcing acupuncture group， and an inhibitor group， with 6 rabbits in each group. A cold-dampness RA rabbit model was established using complete Freund’s adjuvant-ovalbumin mixture combined with an artificial climate chamber. Rabbits of the heat-reinforcing acupuncture group received heat-reinforcing acupuncture at ST36 once daily for 14 d. Rabbits of the inhibitor group was intraperitoneally injected with the mitochondrial division inhibitor Mdivi-1 （2.5 mg/kg） once every 2 d for a total of 7 times. Rabbits of the normal and model groups were handled and restrained in the same manner but received no other interventions. Knee arthritis scores， mechanical pain thresholds， and joint circumference were assessed after modeling and intervention. Hematoxylin-eosin （HE） staining was used to observe pathological changes in knee synovial tissue. Transmission electron microscopy was used to observe the ultrastructure of mitochondria in synovial cells. Luciferase method was used to measure ATP content in knee synovial tissue. Immunofluorescence staining was used to detect mitochondrial reactive oxygen species （mtROS） expression and Drp1/Mff co-localization in synovial tissue， and Western blot was used to measure the relative expression levels of phosphorylated（p）-Drp1 s616/Drp1 and Mff protein.ResultsCompared with the normal group， the model group showed increased arthritis scores， joint circumference， mtROS expression， p-Drp1 s616/Drp1 and Mff protein expression， and Drp1/Mff co-localization in knee synovial tissue（P<0.01）， while mechanical pain thresholds and ATP content in synovial tissue were significantly decreased （P<0.01）. Following acupuncture intervention， compared with the model group， the heat-reinforcing acupuncture group and the inhibitor group showed significantly reduced arthritis scores， joint circumference， mtROS expression ， p-Drp1 s616/Drp1 and Mff protein expression， and Drp1/Mff co-localization in synovial tissue（P<0.01）， while mechanical pain thresholds and ATP content in synovial tissue were significantly increased （P<0.01）. In the model group， synovial tissue showed significant synovial cell proliferation， with visible inflammatory cell infiltration and synovial matrix proliferation in the synovium； synovial cells had indistinct mitochondrial cristae， mitochondrial swelling/rupture， and mitochondrial fragmentation into small bodies. These changes in synovial tissue were alleviated in both the heat-reinforcing acupuncture group and the inhibitor group.ConclusionHeat-reinforcing acupuncture can significantly improve symptoms in RA rabbits with cold-dampness and enhance mitochondrial quality in synovial cells， which may be related to the inhibition of Drp1/Mff pathway activity.关键词：Rheumatoid arthritis;Heat-reinforcing acupuncture;Mitochondrial quality;Dynamin-related protein 1/mitochondrial fission factor pathway62|30|0更新时间：2025-11-04
- “电针激活AMPK改善糖尿病胃轻瘫线粒体功能，抑制NLRP3炎性反应。”摘要：ObjectiveTo observe whether the effect of electroacupuncture（EA） on the improvement of NOD-like receptor protein 3 （NLRP3）-mediated inflammation and gastric motility in diabetic gastroparesis （DGP） rats is exerted through the activation of AMP-activated protein kinase（AMPK） and the protection of mitochondrial homeostasis， so as to explore the mechanism of EA in the prevention and treatment of DGP.MethodsForty SD rats were randomly divided into the control group and modeling group， and the ones in modeling group were injected with 2% streptozotocin and fed with high-glucose and high-fat diet irregularly. The 20 successfully modeled rats were randomized into the model group and EA group with 10 rats each. Rats in the EA group received EA at “Zusanli” （ST36）， “Liangmen” （ST21） and “Sanyinjiao” （SP6） with sparse and dense waves of 20 Hz/100 Hz for 15 min， while rats in the control and model group received bundled controls. Blood glucose， general symptoms， gastric emptying rate and small intestinal propulsion rate were observed in each group. Hematoxylin-eosin（HE） staining was used to observe the pathological changes of gastric antrum tissues. Western blot was used to detect the protein expression of AMPK， phosphorylated AMPK （p-AMPK）， and NLRP3 in gastric antrum tissue， and transmission electron microscopy was used to observe the mitochondrial ultrastructure in gastric antrum cells. Dihydroethidium （DHE） fluorescent probe was used to detect the level of reactive oxygen species （ROS）， the JC-1 method was used to detect changes in mitochondrial membrane potential， while a biochemical assay kit was employed to measure adenosine triphosphate （ATP） levels in gastric antrum tissue.ResultsAfter modeling， compared with the control group， the blood glucose levels and general symptom scores of rats in the modeling group were significantly increased （P<0.01） while gastric emptying rate and intestinal propulsion rate were significantly decreased（P<0.01）. After intervention， compared with the control group， blood glucose and symptom scores remained elevated， gastric emptying rate and intestinal propulsion rate were significantly decreased （P<0.01） in the model group， while gastric antrum tissues showed obvious inflammatory damage. Meanwhile， the model group exhibited decreased AMPK and p-AMPK protein expression levels and increased NLRP3 protein expression level（P<0.01）， mitochondrial structure was severely damaged， with an increase in the intracellular ROS content （P<0.01）， and a decrease in the mitochondrial membrane potential level and ATP content （P<0.01）. Compared with the model group， rats in the EA group had lower blood glucose levels and general symptom scores （P<0.05） and higher gastrointestinal propulsion （P<0.01）， and EA had improved gastric histopathology. The expression of AMPK and p-AMPK proteins were increased while NLRP3 protein was decreased in gastric antrum tissue （P<0.01）. The ultrastructure of mitochondria was improved， and ROS content was decreased （P<0.01）， the mitochondrial membrane potential level and ATP content were elevated （P<0.01）.ConclusionEA may improve DGP by activating AMPK to improve damaged mitochondrial structure and function and promote the restoration of mitochondrial homeostasis， thereby inhibiting NLRP3 inflammasome and its mediated inflammation in gastric antrum tissues.关键词：Electroacupuncture;Diabetic gastroparesis;Mitochondria;AMP-activated protein kinase;Nod-like receptor protein 392|54|0更新时间：2025-11-04
- “最新研究发现，艾灸通过调节肥胖小鼠脂肪组织UCP1、Prdm16、PGC-1α表达及下丘脑cAMP/PKA/CREB信号通路，改善肥胖和脂质代谢。”摘要：ObjectiveTo explore the mechanisms of moxibustion in treating obese mice through its regulatory effects on mitochondrial brown adipose tissue uncoupling protein-1 （UCP1）， PR domain-containing protein 16 （Prdm16）， and peroxisome proliferator-activated receptor gamma coactivator 1-alpha （PGC-1α） in abdominal adipose tissue， and the cyclic adenosine monophosphate （cAMP）/protein kinase A （PKA）/cAMP response element-binding protein （CREB） signaling pathway in the hypothalamus of obese mice.MethodsC57BL/6J mice were randomly divided into the control group， model group， moxibustion group， and sham control group， with 10 mice in each group. An obese mouse model was established by feeding a high-fat diet. Moxibustion was applied mildly at a distance of 3—5 cm from the “Zhongwan” （CV12） with a temperature maintained at （46±1）℃ in the moxibustion group. In the sham control group， moxibustion was applied at a distance of 8—10 cm from CV12 with a temperature maintained at （38±1）℃. Changes in body weight and food intake were observed before and after treatment. Enzyme-linked immunosorbent assay （ELISA） was used to measure the levels of serum triglycerides （TG）， total cholesterol （TC）， and hypothalamic cAMP. The mass of abdominal white adipose tissue （WAT） was assessed. Histopathological changes in adipose tissue were observed using hematoxylin-eosin （HE） staining. Real-time fluorescence quantitative PCR and Western blot were employed to detect the mRNA and protein expression of UCP1， Prdm16， and PGC-1α in abdominal adipose tissue， as well as the expression of PKA and CREB in the hypothalamus.ResultsCompared with the control group， the model group showed increased body weight， food intake， serum TC and TG levels （P<0.01）， as well as increased abdominal WAT mass （P<0.01）， and significantly enlarged adipocyte volume in abdominal adipose tissue. Mice in the model group showed decreased mRNA and protein expression of UCP1， Prdm16， and PGC-1α in abdominal adipose tissue， as well as decreased mRNA and protein expression of PKA and CREB in the hypothalamus （P<0.01）， along with decreased levels of hypothalamic cAMP （P<0.01）. Compared with the model group and sham control group， the moxibustion group showed decreased body weight and food intake （P<0.01， P<0.05）， decreased serum TC and TG levels， and abdominal WAT mass （P<0.01）， along with reduced adipocyte diameter in abdominal adipose tissue. Moreover， compared with the model group and sham control group， the moxibustion group showed increased mRNA and protein expression of UCP1， Prdm16， and PGC-1α in abdominal adipose tissue， and the mRNA and protein expression of PKA and CREB in the hypothalamus （P<0.01）， as well as increased levels of hypothalamic cAMP （P<0.01）.ConclusionMoxibustion can enhance the mRNA and protein expression of UCP1， Prdm16， and PGC-1α in WAT of obese mice， improving obesity and lipid metabolism. Its mechanism may involve the regulation of the hypothalamic cAMP/PKA/CREB signaling pathway.关键词：Obesity;Moxibustion;Hypothalamus;Adipose tissue70|29|0更新时间：2025-11-04
- “最新研究发现，电头针能减轻缺血性脑卒中大鼠的炎性损伤，改善神经功能缺损，缩小脑梗死体积，可能通过调控PPARγ/NF-κB信号通路发挥作用。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） of scalp acupoints on peroxisome proliferator-activated receptor γ （PPARγ）， phosphorylated nuclear factor kappa-B p65 （p-NF-κB p65）， interleukin （IL）-1β and IL-4 as well as CD16 and CD206 in the ischemic cortical tissues of rats with ischemic stroke （IS），so as to explore its mechanisms underlying alleviation of inflammatory damage in ischemic stroke.MethodsMale SD rats were randomly assigned to sham surgery， model， EA， and EA+ inhibitor groups， with 12 rats in each group. The IS model was established by occlusion of the middle cerebral artery with suture-embolus in reference with the modified Zea-Longa method. EA （2 Hz/ 100 Hz， 1 mA） was applied to bilateral “Dingnie Qianxiexian” （MS6） for 30 min， once a day for 7 consecutive days. Rats of the EA+inhibitor group received intraperitoneal injection of GW9662 solution （10 mg/mL， an inhibitor of PPARγ）. The neurological deficit severity （0-3 points） was assessed using Zea-Longa score， modified neurological severity score （mNSS） and neurobehavioral score before and after the intervention. The cerebral infarction volume was determined using 2， 3， 5-triphenyltetrazolium chloride （TTC） staining， and the expression of IL-1β， IL-6， tumor necrosis factor-α （TNF-α）， IL-4， IL-10， and transforming growth factor-β （TGF-β） in the ischemic cortex was detected using immunohistochemistry. The immunofluorescence double labeling of microglial markers CD16 and CD206 in the ischemic cortex was observed. Western blot was used to detect the expression of PPARγ， p-NF-κB p65， inducible nitric oxide synthase （iNOS）， and arginase1 （ARG1） proteins in the ischemic cortex.ResultsCompared with the sham surgery group， the Zea-Longa score， mNSS score and neurobehavioral score， cerebral infarction volume， and the expression levels of p-NF-κB p65， iNOS， the number of CD16/IBA1 double-labelled cells， and the immunoactivity of IL-1β， IL-6 and TNF-α were significantly increased （P<0.01）， while the expression of PPARγ and ARG1 proteins， number of CD206/IBA1 double-labelled cells， and the immunoactivity of IL-4， IL-10 and TGF-β were significantly decreased （P<0.01） in the model group. After the intervention， in comparison with the model group， the Zea-Longa score， mNSS score and neurobehavioral score， the cerebral infarction volume， number of CD16/IBA1 double-labelled cells， and the immunoactivity of IL-1β， IL-6 and TNF-α， and the expression levels of p-NF-κB p65， iNOS， number of CD16/IBA1 double-labelled cells， and the immunoactivity of IL-1β， IL-6 and TNF-α were considerably decreased （P<0.01）， whereas the expression levels of PPARγ and ARG1 proteins， the number of CD206/IBA1 double-labelled cells， and the immunoactivity of IL-4， IL-10 and TGF-β were strikingly increased （P<0.01） in the EA group. After administration of GW9662， the effects of EA were eliminated in reducing the Zea-Longa score， mNSS score and neurobehavioral score， in downregulating the expression of iNOS， p-NF-κB p65， IL-1β and TNF-α （P<0.01）， and in upregulating the expression of ARG-1， PPARy， and IL-4， and the number of CD206/IBA1 double-labelled cells （P<0.01） in the EA+inhibitor group.ConclusionEA of MS6 can alleviate the degree of neurological deficit and reduce the volume of cerebral infarction in IS rats， which may be related to its functions in regulating the PPARγ/NF-κB signaling pathway and in promoting the transformation of microglia towards M2 phenotype.关键词：Acute ischemic stroke;Electroacupuncture;Scalp acupoint;Neurological deficit;PPARγ/NF-κB signaling67|21|0更新时间：2025-11-04
- “最新研究发现，艾灸通过上调CaMKⅡ表达，激活RyR3通道，改善阿尔茨海默病小鼠学习记忆能力。”摘要：ObjectiveTo explore the mechanism by which moxibustion promotes synaptic plasticity of hippocampal neurons and improves learning and memory in mice with Alzheimer’s disease （AD） based on the calmodulin-dependent protein kinase Ⅱ （CaMKⅡ）/ryanodine receptor （RyR）3 pathway.MethodsForty APP/PS1 mice were randomly divided into the model （n=15）， moxibustion （n=25） groups， and 15 C57BL/6J mice served as the blank control group. The mice in the moxibustion group were given moxibustion at the “Baihui”（GV20） and “Yongquan”（KI1） acupoints， 30 minutes each time， once a day. A course of treatment lasted for 5 days， and there were a total of 4 courses of treatment. Ten mice from the moxibustion group were randomly selected as the moxibustion+inhibitor group， and were intraperitoneally injected with the CaMKⅡ protein inhibitor KN-93 （2 mg/100 g） one day before sample collection on the basis of moxibustion. After the treatment， the shuttle box test was used to evaluate the learning and memory ability of the mice. Transmission electron microscopy was used to detect the changes in the ultrastructure of synapses in the CA1 region of the hippocampus. Western blot was used to detect the expression levels of β-amyloid protein （Aβ） and CaMKⅡ protein in the hippocampus of the mice. Immunofluorescence staining was used to detect the positive expression of CaMKⅡ in the hippocampus and the co-expression of RyR3/PSD95.ResultsCompared with the blank control group， the number of active avoidance responses， the protein expression and positive expression of CaMKⅡ in the hippocampus， and the percentage of the co-expression area of RyR3/PSD95 of the mice in the model group were decreased （P<0.01， P<0.05）， and the number of synaptic vesicles in the CA1 region of the hippocampus was decreased； while the expression of Aβ protein in the hippocampus was increased （P<0.01）. Compared with the model group， the number of active avoidance responses， the protein expression and positive expression of CaMKⅡ in the hippocampus， and the co-expression area percentage of RyR3/PSD95 of the mice in the moxibustion group were increased significantly （P<0.01）， and the number of synaptic vesicles in the CA1 region of the hippocampus was increased； while the expression of Aβ protein was decreased （P<0.05）. Compared with the moxibustion group， the number of active avoidance， the positive expression of CaMKⅡ and the co-expression area percentage of RyR3/PSD95 in the moxibustion+inhibitor group were decreased （P<0.01）， and the number of synaptic vesicles in the CA1 region of the hippocampus was decreased.ConclusionMoxibustion can improve the learning and memory ability of AD model mice， and its mechanism may be related to up-regulating the expression of CaMKⅡ， activating the endoplasmic reticulum RyR3 channel， inducing the release of Ca2+， and then promoting neuronal synaptic plasticity.关键词：Moxibustion;Alzheimer’s disease;Synaptic plasticity;Calcium/calmodulin-dependent protein kinase Ⅱ83|16|0更新时间：2025-11-04
- “最新研究显示，超声精准定位引导次髎/中髎电针治疗脊髓损伤后尿潴留，定位更准确，疗效更确切。”摘要：ObjectiveTo observe the correlation between the localization accuracy and therapeutic effect of electroacupuncture （EA） at Ciliao （BL32） and Zhongliao （BL33） guided by ultrasonic precise positioning in treatment of urinary retention after spinal cord injury.MethodsFifty-two patients with urinary retention after spinal cord injury were randomly divided into a control group and an observation group， with 26 cases in each group. In the control group， the bladder function training method and EA at BL32 and BL33 were adopted for 4 weeks； and in the observation group， besides the bladder function training method， EA was delivered at BL32 and BL33 under the ultrasonic precise positioning for 4 weeks. The sonographic appearance at BL32 and BL33 was observed. The score of international lower urinary tract syndrome symptom scale （LUTS） was recorded， and voiding diary， residual urine volume， and urinary tract infection were compared before and after treatment between the two groups. By comparing the clinical evaluation of the positioning accuracy of BL32 and BL33 between the two groups， and analyzing the correlation between the indicators of positioning accuracy of BL32 and BL33 and LUTS score using Pearson correlation test， the accuracy of EA at BL32 and BL33 guided by ultrasonic precise positioning was evaluated， as well as its correlation with the therapeutic effect on urinary retention after spinal cord injury.ResultsThrough intention analysis， the LUTS score after treatment was reduced significantly compared with that before treatment in either group （P<0.05， P<0.01）， and the score in the observation group was remarkably lower than that of the control group （P<0.01）. Both groups showed the improvement in urination frequency， maximum urine volume， average urine volume and residual urine volume after treatment in comparison with those before treatment （P<0.05， P<0.01）； the improvement in these indicators in the observation group was significantly better than that of the control group （P<0.05）. The positive rate of urinary tract infection （7/26， 26.9%） after treatment was remarkably reduced in comparison with that （16/26， 61.5%） before treatment in the observation group （P<0.01）， and was better than that （12/26， 46.2%） of the control group （P<0.01）. Regarding the clinical evaluation indicators related to the accuracy of positioning BL32 and BL33， the results in the observation group were superior to the control group （P<0.01）； and these indicators were positively correlated with LUTS score after treatment in the two groups （r=0.625， P<0.01）.ConclusionUltrasound guidance is conductive to the accurate localization of BL32 and BL33. EA at these two acupoints under the ultrasound obtains the more accuracy on the acupoint location and higher therapeutic effect on urinary retention after spinal cord injury.关键词：Spinal cord injury;Urinary retention;Ultrasound;Ciliao;Zhongliao;Electroacupuncture;Accuracy55|37|0更新时间：2025-11-04
- “在恢复期特发性面神经麻痹治疗领域，研究显示，结合经颅重复针刺百会及健侧运动区下1/5的电针治疗，能显著提高疗效，改善患者面部肌肉瘫痪等症状。”摘要：ObjectiveTo observe the clinical efficacy of transcranial repeated acupuncture at Baihui （GV20） and the lower 1/5 of the healthy-side motor area in treating idiopathic facial paralysis （IFP） during the recovery phase.MethodsA total of 72 IFP patients in the recovery phase were randomly divided into control group （36 cases， 3 dropped off） and treatment group （36 cases， 2 dropped off）. Both groups received basic western medical treatment， including neurotrophic drugs， until the end of the treatment course. The control group underwent conventional electroacupuncture （EA） for 30 min， followed by an additional 10 min of needle retention， totaling 40 min per session. The treatment group received the same 30 min EA as the control group， with the addition of transcranial repeated acupuncture involving manual twisting of needles at GV20 and the lower 1/5 of the healthy-side motor area for 5 min each， also totaling 40 min per session. Both groups were treated once daily， with 6 sessions constituting 1 treatment course. After each course， a 1-day break was taken， and treatment continued for 4 consecutive courses. Before and after treatment， efficacy was evaluated using the House-Brackmann （H-B） facial nerve grading scale， facial disability index （FDI） scale （including physical function ［FDIP］ and social function ［FDIS］ subscales）， and a traditional Chinese medicine （TCM） symptom efficacy scoring scale. Clinical outcomes were compared between the 2 groups.ResultsCompared with pre-treatment levels， both groups showed improvement in H-B grading （P<0.05，P<0.01）， increased FDIP scores （P<0.05）， and decreased FDIS and TCM symptom scores （P<0.05） after treatment. Post-treatment， the treatment group exhibited significantly greater improvement in H-B grading （P<0.05）， higher FDIP scores （P<0.05）， and lower FDIS and TCM symptom scores （P<0.05） compared to the control group. The total effective rate was 94.12% （32/34） in the treatment group and 87.88% （29/33） in the control group， with the treatment group demonstrating statistically superior efficacy （P<0.05）.ConclusionCombining transcranial repeated acupuncture at GV20 and the lower 1/5 of the healthy-side motor area with conventional EA enhances clinical efficacy， significantly improves facial muscle paralysis and related symptoms such as facial deviation， food retention， and taste dysfunction in IFP patients during the recovery phase.关键词：Idiopathic facial paralysis;Transcranial repeated acupuncture;Electroacupuncture;Baihui （GV20）;Lower 1/5 of the healthy-side motor area44|30|0更新时间：2025-11-04
- “在真实医疗环境中，研究显示“通督调神”针法能有效治疗失眠症，缓解疲劳和焦虑情绪，且治疗次数对疗效有显著影响。”摘要：ObjectiveTo investigate the efficacy of “Tongdu Tiaoshen” needling （draging the Governor Vessel and normalizing mental activities） in the treatment of insomnia in the clinical real world and to explore the influencing factors of clinical efficacy.MethodsA total of 227 patients with insomnia were given “Tongdu Tiaoshen” （freeing the Governor Vessel and regulating the spirit） needling， with electroacupuncture （EA） applied at Baihui （GV20）， Yintang （GV24+）， bilateral Shenmen （HT7）， and bilateral Sanyinjiao （SP6） for 30 min each time. Based on the number of treatments， the patients were divided into 2 cohorts： the < 6 times group （227 cases） and the≥6 times group （74 cases）. Then， propensity score matching was used to balance the confounding factors between the cohorts. Patients with < 6 treatments within 4 weeks were included in the low-frequency group （37 cases）， and patients with≥6 treatments were included in the high-frequency group （37 cases）. Propensity score matching was used to balance the confounding factors between the cohorts. Before and after treatment， the Pittsburgh sleep quality index （PSQI）， fatigue severity scale （FSS）， self-rating depression scale （SDS）， and self-rating anxiety scale （SAS） scores of the patients were evaluated. The therapeutic effect was assessed.ResultsFollowing the treatment， the PSQI and FSS scores of the high-frequency treatment group and the low-frequency treatment group were all decreased （P<0.05）， while the FSS score of the high-frequency treatment group was lower than that of the low-frequency treatment group （P<0.05）. There were no significant differences in SDS scores between the two groups. The high-frequency treatment group showed a significant decrease （P<0.05） in SAS scores after treatment than that before treatment， whereas no significant difference was observed in the low-frequency group， additionally， the high-frequency treatment group was lower than the low-frequency treatment group （P<0.05）.Conclusion“Tongdu Tiaoshen” needling can effectively treat insomnia， relieve fatigue and anxiety in patients with insomnia. The number of treatment significantly affects the clinical efficacy in treating insomnia.关键词：“Tongdu Tiaoshen” needling;Insomnia;Real-world study;Efficacy evaluation67|44|0更新时间：2025-11-04
- “针灸临床试验样本量估计常见问题探讨，为研究者提供参考。”摘要：Sample size estimation is a crucial step in clinical trial design， directly impacting the reliability and reproducibility of research findings. Researchers currently face several methodological challenges when estimating sample sizes for acupuncture clinical trials， such as： whether sample size estimation is necessary for pilot trial； what parameters are required for sample size estimation； whether sample size estimation methods differ among various types of hypothesis tests； how to perform sample size estimation with multiple primary outcomes or multiple groups； and how to standardize the reporting of sample size estimation. Based on these， this article addressed common problems of sample size estimation in acupuncture pilot trial and confirmatory trial， put forward solving strategies accordingly， in order to provide reference for clinical researchers in acupuncture clinical trial sample size estimation.关键词：Acupuncture;Randomized controlled trial;Sample size estimation;Pilot trial;Confirmatory trial74|26|0更新时间：2025-11-04
- “最新研究发现，针灸治疗颈源性头痛核心穴位组合为风池、百会、天柱、颈夹脊、阿是穴，为临床治疗提供新思路。”摘要：ObjectiveTo investigate the clinical application characteristics and rules of acupuncture-moxibustion therapy in treatment of cervicogenic headache （CEH） based on complex network analysis techniques.MethodsA comprehensive and systematic literature search was conducted across the China National Knowledge Infrastructure （CNKI）， Wanfang Data Knowledge Service Platform （Wanfang）， VIP Chinese Science and Technology Periodical Database （VIP）， SinoMed Chinese Biomedical Literature Database， PubMed， EMbase， and Cochrane Library， from their inception to May 31， 2024， to retrieve relevant articles on acupuncture-moxibustion treatments for CEH. A database was constructed using Microsoft Excel 2019 to statistically analyze and create comprehensive pivot tables for the frequency of acupoint utilization， meridian attribution， syndrome differentiation， and therapeutic protocols. Furthermore， the Apriori algorithm within SPSS Modeler 18.0 was employed to perform an association rule analysis on the primary acupoint prescriptions. Cytoscape 3.7.2 software was utilized to visualize the complex network co-occurrence patterns.ResultsA total of 311 eligible articles were included， yielding 452 acupuncture prescriptions that encompassed 146 acupoints with a cumulative utilization frequency of 2 349. The top five most frequently employed acupoints were Fengchi （GB20）， Tianzhu （BL10）， Jingjiaji （EX-B2）， Ashi points， and Baihui （GV20）. The predominant meridians targeted were the gallbladder meridian， bladder meridian， governor vessel， triple energizer meridian， and stomach meridian. Specific points， such as confluent points and five-shu points， were frequently utilized. Acupoints selected are mainly distributed on the head， neck， upper extremities， and lower extremities. The even veinforcing-reducing needling technique was dominant in acupuncture manipulation. The most commonly adopted single technique is filiform needling or electroacupuncture. Regarding the comprehensive therapy， filiform needling and tuina was combined. The highest co-occurrence frequency was observed between BL10 and GB20， and the close interconnection was presented among GB20， GV20， BL10， EX-B2 and Ashi （Extra）； and these points demonstrated the strongest connectivity to other acupoints. Five syndromes/patterns of traditional Chinese medicine were involved. Shaoyang syndrome was treated with Shuaigu （GB8）， Waiguan （TE5）， and Taiyang （EX-HN5）， liver-yang hyperactivity syndrome was with Taichong （LR3）， Xingjian （LR2）， and Taixi （KI3）； wind-cold syndrome was with Dazhui （GV14）， Fengmen （BL12）， and Hegu （LI4）； Taiyang syndrome was with BL10， Fengfu （GV16）， and Houding （EX-HN9）； and Jueyin syndrome was with GV20， LR3， and Sishencong （EX-HN1）.ConclusionThe core acupoint combination of acupuncture and moxibustion for CEH comprises GB20， GV20， BL10， EX-B2 and Ashi points. In clinical practice， the nearby points are principal， combined with the distal points， as well as those selected by the upper-lower combination； and the meridian differentiation is emphasized. On the basis of core acupoints， the supplementary acupoitns are selected accordingly to optimize therapeutic outcomes.关键词：Cervicogenic headache;Acupuncture and moxibustion;Complex network;Data mining;Clinical application75|418|0更新时间：2025-11-04
- “正电子发射型计算机断层显像技术（PET-CT）融合功能代谢显像和解剖结构显像，广泛应用于针刺效应机制研究，证实针灸作用效应的脑功能机制，为针刺研究提供直观可视化检测手段和精准数据依据。”摘要：Positron emission computed tomography （PET-CT）， which combines functional metabolism imaging and anatomical structure imaging， represents the highest level of nuclear medicine imaging technology， and is increasingly used in the study of acupuncture effect mechanism. In the present paper， we review the recent progress of application of PET-CT technology in acupuncture clinic and experimental study， and conclude that the advantages of PET-CT technology are that it can confirm the acupuncture effect from the level of brain functional activities and structure， including the brain functional mechanism of acupoint effect specificity， acupoint compatibility effect， acupuncture Deqi effect， and bidirectional regulation effect of acupuncture， etc. At present， PET-CT technology is mainly used to explore the central mechanism of acupuncture in improving brain diseases， and is also commonly used in gastrointestinal diseases， cardiovascular diseases and gynecological diseases. PET-CT technology provides intuitive and visual detection means and a more direct and accurate data basis for acupuncture research， which can reflect the overall regulation characteristics and advantages of acupuncture with multi-targets， multi-pathways and multi-levels， and provides effective means and technical support for the in-depth study on the underlying mechanisms of acupuncture effects.关键词：PET-CT;Acupuncture;Mechanism;Review55|30|0更新时间：2025-11-04
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Research progress on the mechanism of acupuncture in antidepressant effects by regulating astrocytes AI导读
“据最新研究报道，针刺治疗抑郁症通过改善星形胶质细胞形态、调节细胞间相互作用等机制发挥作用，为抑郁症治疗提供新思路。”摘要：Astrocytes， as one of the major glial cells in the central nervous system， perform a wide range of homeostatic functions in the brain. A reduction in the number or dysfunction of astrocytes impairs neural homeostatic functions， thereby contributing to the occurrence and development of depression. Acupuncture has a proven efficacy in treating depression. Based on a large number of recent studies on the regulation of astrocytes by acupuncture for antidepressant effects， this paper reviews the mechanisms of acupuncture in treating depression from three aspects： improving the morphology and quantity of astrocytes， regulating astrocyte-neuron interactions， and modulating astrocyte-microglia crosstalk， and proposes directions for future research. It is expected to provide new insights for the targeted regulation of astrocytes in the treatment of depression.关键词：Depression;Acupuncture;Astrocytes;Mechanism63|41|0更新时间：2025-11-04
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