最新刊期
卷 50 ， 期 11 ， 2025
- “最新研究发现，电针心经通过影响杏仁核改善急性心肌缺血大鼠心脏功能和线粒体损伤，为治疗心肌缺血提供新思路。”摘要：ObjectiveTo investigate the effect of electroacupuncture （EA） of the Heart Meridian on ischemic myocardial injury， left cardiac function， myocardial reactive oxygen species （ROS）， and expression levels of mitochondrial injury related proteins and genes as mitofusin 2 （Mfn2）， calcium/calmodulin-dependent protein kinase II （CaMKⅡ）， and dynamin-related protein 1 （Drp1） in rats with acute myocardial ischemia （AMI）， and lesions of the anterior/posterior basolateral amygdala （a/pBLA）， so as to assess its mechanisms underlying improvement of AMI and involvement of BLA in EA-induced alleviation of myocardial mitochondrial injury.MethodsMale SD rats were randomly divided into sham operation， AMI model， EA， EA + aBLA lesion， and EA + pBLA lesion groups （n=12 in each group）. The AMI model was established by occlusion of the left anterior descending （LAD） branch of the coronary artery. In the sham group， only a thoracotomy was performed without ligation of LAD. For rats of the amygdaloid lesion groups， microinjection of kainic acid （50 nL） into the pBLA and aBLA was conducted 3 d before the surgery to induce nucleus lesions. EA （2 Hz， 1—2 mA） was applied to“Shenmen”（HT7） to“Tongli”（HT5） segment of the Heart Meridian for 30 min， once daily， for a total of 3 d. Electrocardiography was used to record ST segment displacement of the standard limb lead II （ECG-STII） and low frequency/high frequency （LF/HF） ratio of the heart rate variability changes. Echocardiography was used to evaluate various indexes of cardiac function， including the left ventricular ejection fraction （LVEF）， left ventricular fractional shortening （LVFS）， left ventricular internal diameter in systole （LVIDs）， and left ventricular internal diameter in diastole （LVIDd）. The myocardial infarction area was measured after TTC staining. H.E. & Masson staining were used to examine histopathological changes of the myocardial tissue. Transmission electron microscopy was utilized to observe the ultrastructure of cardiomyocytes and mitochondria. Flow cytometry was used to detect ROS in cardiomyocytes. Real-time quantitative PCR was employed to measure the expression of Mfn2， CaMKⅡ， and Drp1 mRNA expressions in the myocardial tissue. Western blot was used to detect the expression of Mfn2， Drp1， phosphorylated（p）-Drp1， CaMKⅡ， and p-CaMKⅡ proteins in myocardial tissue.ResultsCompared to the sham group， the model group had a significant increase in the levels of ECG-STII， LF/HF ratio， LVIDs， LVIDd， proportion of myocardial infarction area， ROS fluorescence intensity， mRNA expression levels of Drp1 and CamkⅡ， and protein expression levels of p-Drp1 and p-CaMKⅡ （P<0.01）， and a decrease in the levels of LVEF， LVFS and expression of Mfn2 protein and mRNA （P<0.01）. In contrast to the model group， both the increase and decrease of the indexes mentioned above were reversed by both EA and EA+aＢLA lesion （P<0.05， P<0.01）， but not by EA+pBLA lesion. No significant differences were found between the EA and EA+aBLA lesion groups in the increased and decreased levels of the indexes mentioned above. The levels of ECG-STII， LF/HF ratio， LVIDs， LVIDd， proportion of myocardial infarction area， ROS fluorescence intensity， expression of Drp1 and CaMKⅡ mRNAs， and p-Drp1 and p-CaMKⅡ proteins were significantly lower in the EA group than in the EA+pBLA lesion group （P<0.01， P<0.05）， and the levels of LVEF， LVFS， expression of Mfn2 mRNA and protein were obviously higher in the EA group than in the EA+pBLA lesion group （P<0.01， P<0.05）. H.E. & Masson staining showed swelling， rupture， necrosis， nuclear abnormal changes， inflammatory infiltration， increased collagen deposition and obvious fibrosis of the myocardial cells in the model group. Electron-microscopic observation showed sarcomere disorder， swollen mitochondria， disrupted cristae， with appearance of autophagosomes in cardiomyocytes in the model group. The damage severity of cardiomyocytes was relatively milder in both EA and EA+aBLA groups （including well demarcated mitochondria， reduction in the number of autophagosomes， etc.）， but not in the EA+pBLA group.ConclusionEA can improve ischemic myocardial injury in rats with AMI， which may be related to its function in reducing endoplasmic reticulum stress level to improve mitochondrial dysfunction， and the BLA particularly the pBLA participates in EA-induced improvement of AMI.关键词：Acute myocardial ischemia;Electroacupuncture;Basolateral amygdala;Mitochondria;Oxidative stress74|34|0更新时间：2025-11-17
- “最新研究发现，电针通过抑制cGAS-STING信号通路介导的铁自噬，改善大脑中动脉闭塞再灌注大鼠学习记忆能力。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on learning and memory in rats with middle cerebral artery occlusion/reperfusion （MCAO/R） by regulating ferritinophagy through the cyclic GMP-adenosine synthase （cGAS） - stimulator of interferon genes （STING） signaling pathway.MethodsSD rats were randomly divided into a normal group， a model group， and an EA group. Except for the normal group， all rats underwent MCAO/R by suture occlusion. The EA group received treatment at “Shenting” （GV24） and “Baihui” （GV20） for 30 min once daily for 14 d. The Zea-Longa score was used to assess neurological deficits in rats. The Morris water maze test and the new object recognition test were used to evaluate the learning and memory abilities of rats. The elevated plus maze test was used to evaluate the anxiety state of rats. The TTC staining was used to measure the infarct volume of rats. The HE staining method was used to observe pathological changes in rat’s brain tissue. Iron deposition in the hippocampus was assessed with Prussian blue staining. Mitochondrial morphology in the hippocampus of rats was observed using transmission electron microscopy. Western blot was used to detect the protein expression levels of cGAS， STING， nuclear receptor coactivator 4 （NCOA4）， ferritin heavy chain 1 （FTH1）， P62， Beclin1， and the ratio of micro tubule-associated protein 1 light chain 3 （LC3） Ⅱ/ LC3Ⅰ in the hippocampus of rats. Real-time fluorescence quantitative PCR was used to detect the mRNA expression levels of interferon regulatory factor 3 （IRF3）， TANK-binding kinase 1 （TBK1）， Interleukin-10 （IL-10）， Interleukin-1β （IL-1β）， and tumor necrosis factor-α （TNF-α） in the hippocampus of rats.ResultsCompared with the normal group， the neurological deficit scores， infarct volume， escape latency， time to explore old objects， and the expression levels of cGAS， STING， NCOA4， Beclin1， and the ratio of LC3Ⅱ/ LC3Ⅰ proteins， as well as IRF3， TBK1， IL-1β， and TNF-α mRNAs were increased （P<0.01） in the model group； the number of times crossing the original platform， the time spent exploring new objects， cognitive index， the percentage of time spent in the open arms， the percentage of times spent in the open arms， and the expression levels of FTH1， P62 proteins， and IL-10 mRNA were decreased （P<0.01）. The model group displayed sparse hippocampal CA1 neurons， fewer cells， disorganized structure， and evident brownish iron deposition. Mitochondria appeared swollen with disrupted cristae. Compared with the model group， the EA group showed reduced neurological deficit scores， infarct volume， escape latency， time to explore old objects， and the expression levels of cGAS， STING， NCOA4， Beclin1， and the ratio of LC3Ⅱ/ LC3Ⅰ， IRF3， TBK1， IL-1β， and TNF-α mRNAs （P<0.05， P<0.01）. Meanwhile， the number of times crossing the original platform， the time spent exploring new objects， cognitive index， the percentage of time spent in the open arms， the percentage of times spent in the open arms， and the expression levels of FTH1， P62 proteins， and IL-10 mRNA increased in the EA group （P<0.01， P<0.05）. The EA group showed improved neuronal arrangement in the hippocampal CA1， with uniform cell spacing and more regular cell morphology. Iron deposition was reduced. Mitochondria demonstrated improved morphology， with less cristae dissolution but no obvious rupture， appearing more normal and structurally intact.ConclusionEA can improve the learning and memory abilities of MCAO/R rats， and its mechanism may be related to the inhibition of ferritinophagy mediated by the cGAS-STING signaling pathway.关键词：Ischemic stroke;Electroacupuncture;cGAS-STING signaling pathway;Ferritinophagy61|409|0更新时间：2025-11-17
- “最新研究发现，电针“关元”“三阴交”能减轻原发性痛经大鼠痛觉敏化，可能通过激活CB2R、抑制TRPV1表达，促进小胶质细胞极化实现。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the expression of cannabinoid receptor type 2 （CB2R）， atypical cannabinoid receptor transient receptor potential vanilloid 1 （TRPV1）， and spinal microglial polarization in rats with primary dysmenorrhea （PDM）， so as to explore the underlying mechanism of EA alleviating PDM pain hyperalgesia.MethodsThirty female SD rats were randomly divided into control， model， and EA groups （n=10 rats per group）. The PDM model was established by subcutaneous injection of estradiol diphenhydrate combined with intraperitoneal injection of oxytocin. Rats in the EA group were treated with EA （50 Hz） at “Guanyuan” （CV4） and bilateral “Sanyinjiao” （SP6） for 20 min， once a day for 10 consecutive days. The number of writhing， writhing score， and writhing latency were observed. The uterine histopathological changes were observed by H.E. staining. The contents of prostaglandin F2α （PGF2α）， prostaglandin E2 （PGE2） in serum and interleukin-6 （IL-6）， interleukin-1β （IL-1β）， tumor necrosis factor-α （TNF-α） and interleukin-10 （IL-10） in spinal cord were detected by ELISA. The protein expressions of CB2R and TRPV1 in spinal cord and uterus， and inducible nitric oxide synthase （iNOS） and arginase-1 （Arg1） in spinal cord were detected by Western blot.ResultsCompared with the control group， the number and score of writhing， pathological injury score， contents of PGF2α and ratio of PGF2α/PGE2 in serum and IL-1β， TNF-α and IL-6 contents in spinal cord， the protein expressions of TRPV1 in both uterine and spinal cord， and iNOS in spinal cord were significantly increased in the model group （P<0.001）， while contents of PGE2 in serum and IL-10 in spinal cord， the expression levels of CB2R in both uterine and spinal cord， and Arg1 in spinal cord were significantly decreased （P<0.001）. In comparison with the model group， the number of writhing， pathological injury score， contents of PGF2α and ratio of PGF2α/PGE2 in serum and IL-1β， TNF-α and IL-6 contents in spinal cord， the protein expressions of TRPV1 in both uterine and spinal cord and iNOS in spinal cord were obviously decreased （P<0.001， P<0.05， P<0.01）， whereas the writhing latency was considerably prolonged （P<0.05）， with elevated contents of PGE2 in serum and IL-10 in spinal cord， and elevated protein expression levels of CB2R in both uterine and spinal cord， and Arg1 in spinal cord （P<0.01，P<0.001） in the EA group. H.E. staining showed degeneration， swelling， and necrosis of endometrial epithelial cells， dilation of glands， stromal hyperemia， and granulocyte infiltration in the model group， which were relatively milder in the EA group.ConclusionEA can alleviate uterine inflammatory response， pathological damage and pain sensitization in PDM rats， which may be related to activating peripheral and central CB2R expression， inhibiting TRPV1 expression， promoting spinal cord microglia polarization from M1 to M2， thus reducing neuroinflammation.关键词：Primary dysmenorrhea;Electroacupuncture;Pain hyperalgesia;Cannabinoid receptor type 2;Transient receptor potential vanilloid 1;Microglia;Neuroinflammation46|46|0更新时间：2025-11-17
- “最新研究发现，隔药饼灸能减轻子宫内膜异位症大鼠痛经症状，抑制异位内膜组织生长，可能通过下调TGF-β/Smad信号通路相关因子表达发挥作用。”摘要：ObjectiveTo investigate the mechanism of medicinal cake-separated moxibustion in inhibiting the adhesion， invasion and angiogenesis of ectopic endometriotic tissues in endometriosis （EMs） rats through transforming growth factor-β1 （TGF-β）/Sma and Mad related proteins （Smad） signaling pathway.MethodsForty-eight female SD rats were randomly divided into blank control， model， medication （gestrinone）， and medicinal cake-separated moxibustion（moxibustion） groups （n=12 in each group）. The model of EMs was established by autologous transplantation method. The rats in the blank control and model groups were treated with normal saline via gavage， and those of the medication group were treated by intragastric gavage of gestrinone （0.25 mg/kg）， once daily for 2 weeks. For rats of the moxibustion group， medicinal cake-separated moxibustion was applied to “Guanyuan” （CV4）， “Qihai” （CV6） and “Zigong”（EX-CA1） for 30 min， once a day for 14 consecutive days. At the end of the treatment， the rats in the model and two treatment groups received intraperitoneal injection of oxytocin for inducing an experimental dysmenorrhea， and the number of writhing in 20 min due to dysmenorrhea was recorded， once daily for 7 consecutive days， and the volume of ectopic endometrium tissue was measured by using a vernier caliper. H.E. staining was used to observe histopathological changes of the ectopic endometrium tissue. The expression levels of TGF-β1， Smad2， Smad3， urokinase-type plasminogen activator （uPA）， vascular endothelial growth factor A （VEGFA）， matrix metalloproteinase （MMP） 9， and MMP2 were detected by immunohistochemistry and Western blot， separately.ResultsCompared with the blank control group， the writhing score and incidence， EMs volume， average optical density values of TGF-β1， Smad2， Smad3， uPA， VEGFA， and MMP9， and the protein expression levels of MMP9， MMP2， TGF-β1， Smad2， Smad3， uPA and VEGFA were significantly up-regulated （P<0.01， P<0.05） in the model group. Compared with the model group， the writhing score and incidence， EMs volume， immunoactivity of TGF-β1， Smad2， Smad3， uPA， VEGFA and MMP9， and the protein expression levels of MMP9， MMP2， TGF-β1， Smad2， uPA and VEGFA were significantly down-regulated （P<0.05， P<0.01） in both medication and moxibustion groups. The effect of moxibustion was apparently superior to that of medication in down-regulating the immunoactivity of Smad2 （P<0.05）， and inferior to that of medication in down-regulating the immunoactivity of MMP9 and VEGFA， and protein expression levels of Smad3， MMP9， MMP2 and uPA （P<0.05）. H.E. staining showed discontinuous epithelial tissue structure accompanied by obvious angiogenesis in the ectopic endometrium of the model group， and relatively loose arrangement of the epithelial nuclei of the lesions with vacuolar degeneration， and increased number of glands in both medication and moxibustion groups.ConclusionMedicinal cake-separated moxibustion can relieve dysmenorrhea and inhibit the development of ectopic endometrium in EMs rats， which may be related to its functions in down-regulating the expressions of TGF-β/Smad signaling related factors， and in inhibiting the adhesion， invasion and angiogenesis of ectopic endometrium tissue.关键词：Medicinal cake-separated moxibustion;Endometriosis;TGF-β/Smad signaling pathway;Tissue adhesion and invasion;Angiogenesis61|59|0更新时间：2025-11-17
- “最新研究发现，“秩边透水道”针法通过抑制JAK2/STAT3信号通路改善卵巢低反应小鼠卵巢功能。”摘要：ObjectiveTo explore the effect of penetrative needling of “Zhibian” （BL54） to “Shuidao” （ST28） on ovarian function in mice with poor ovarian response （POR） by regulating the Janus kinase 2（JAK2）/signal transducers and activators of transcription（STAT）3 signaling pathway.Methods48 female C57BL/6 mice were randomly divided into control， model， acupuncture and inhibitor groups， with 12 mice in each group. The POR model was established by gavage of tripterygium wilfordii polyglucoside suspension （50 mg/kg）. After successful modeling， the mice in the acupuncture group received penetrative needling of BL54 through ST28， with the needle retained for 20 min， once a day for 2 weeks. Mice in the inhibitor group received intraperitoneal injections of AG490 inhibitor solution （1 mg/kg） once daily for 2 weeks. On the final day of intervention， all mice underwent superovulation via intraperitoneal injection of pregnant mare serum gonadotropin （PMSG）， followed by human chorionic gonadotropin （HCG） 48 h later. General conditions and estrous cycles were observed daily. Oocyte yield， ovarian wet weight， and ovarian index were measured. Serum levels of anti-Müllerian hormone （AMH）， follicle-stimulating hormone （FSH）， estradiol （E2）， and luteinizing hormone （LH） were assessed by ELISA. Ovarian morphology was examined via H&E staining， and ultrastructure via transmission electron microscopy. Apoptosis levels in ovarian tissue was detected by TUNEL staining. The expression levels of JAK2， STAT3， and suppressor of cytokine signaling 3 （SOCS3） mRNA were detected by PCR. Protein expression levels of JAK2， phosphorylated （p）-JAK2， STAT3， p-STAT3， SOCS3， B-cell lymphoma-2 （Bcl-2）， and Bcl-2-associated X protein （Bax） were detected by Western blot. Immunohistochemistry was used to detect positive expression of JAK2， p-JAK2， STAT3， and p-STAT3 in ovarian tissue.ResultsCompared with the control group， the rate of estrous cycle disorder， the contents of FSH and LH in serum， the rate of ovarian apoptosis， the expression of JAK2 and STAT3 mRNA， the expression of p-JAK2， p-STAT3 and Bax protein， and the positive expression of p-JAK2 and p-STAT3 in ovarian tissue were significantly increased （P<0.01）； whereas the number of eggs obtained， ovarian wet weight， ovarian index， the contents of AMH and E2， the expression of SOCS3 mRNA， as well as SOCS3 and Bcl-2 protein were significantly decreased （P<0.01） in the model group. Following the intervention， the above-mentioned indexes were all reversed in the acupuncture and inhibitor groups （P<0.01， P<0.05）. The ultrastructure of ovarian was damaged in the model group， which were relatively milder in the acupuncture and inhibitor groups.ConclusionPenetrative needling of BL54 through ST28 can improve the ovarian function in POR mice， which may be associated with its function in inhibiting the phosphorylation of the JAK2/STAT3 signaling pathway.关键词：Zhibian （BL54） through Shuidao （ST28）;Poor ovarian response;JAK2/STAT3 signaling pathway46|21|0更新时间：2025-11-17
- “经皮穴位电刺激（TEAS）通过调节TLR4信号通路，改善睡眠剥夺大鼠免疫功能，增加体重。”摘要：ObjectiveTo observe the effect of transcutaneous electrical acupoint stimulation （TEAS） on the innate immune function of sleep-deprived rats， so as to explore its possible molecular mechanisms underlying improvement of sleep deprivation.MethodsTwenty-four SD rats were randomly divided into control， sleep deprivation model， TEAS and sham TEAS groups， with 6 rats in each group. The sleep deprivation model was established by using the modified multi-platform aqueous environment sleep restriction method. TEAS （2 Hz/100 Hz， 2 mA） was applied to “Beixin Tiaoshen Wuxue” （the back-heart Shen-regulating region）， bilateral “Sanyinjiao”（SP6） and bilateral “Zusanli”（ST36） for 25 min， once daily for 30 successive days. For rats of the sham TEAS group， the stimulating electrodes were fixed as those in the TEAS group but without electric current supplied. After the intervention， the rats’ sleep latency and sleep duration were measured by pentobarbital sodium righting reflex tests. The spleen and thymus indexes were calculated by organ mass coefficient analysis. Hematoxylin-eosin （H.E.） staining was used to observe the histopathological changes of the spleen and thymus tissues. The contents of interleukin （IL）-1β， IL-6， tumor necrosis factor-alpha（TNF-α） and IL-10 in the peripheral blood serum were detected by ELISA. The expression levels of TLR4， MyD88 and NF-κB p65 mRNAs and proteins in the splenic tissues were detected by qPCR and Western blot， separately.ResultsCompared with the rats of the control group， those of the model group showed poorer mental state， fur color and other conditions， with a significant decrease in the body mass from day 7 to 28 after modeling， duration of sleep， index of spleen and thymus， and serum IL-10 content （P<0.05， P<0.01， P<0.001）， and a considerable increase in the sleep latency， contents of serum TNF-α， IL-1β and IL-6， and the expression levels of TLR4， MyD88 NF-κB p65 mRNAs and proteins （P<0.01）. In comparison with the model group and the sham TEAS group， rats in the TEAS group showed better mental state and fur color， with a significant increase in body weight （P<0.01）. Other indicators were also improved to varying degrees （P<0.01， P<0.05）. H.E. staining showed that both the spleen and thymus tissues in the model group had a typical organ damage including disappearance of the boundary between the white pulp and red pulp， decreased area of the white pulp， decreased density of the lymph follicles， irregular shape of the residual follicles， and interruption of the continuity of lymphatic sheath around the arteries which were apparently milder in the TEAS group rather than in the sham TEAS group.ConclusionTEAS can improve sleep and increase body mass in sleep deprivation rats， which may be related to its functions in increasing the index of the spleen and thymus， regulating the balance of pro- and anti-inflammatory cytokine levels and suppressing the activities of TLR4/MyD88/NF-κB signaling in the spleen tissue.关键词：Sleep deprivation;Transcutaneous electrical acupoint stimulation （TEAS）;Innate immunity;Spleen;Thymus;Inflammatory cytokines;TLR4/MyD88/NF-κB signaling49|50|0更新时间：2025-11-17
- “最新研究发现，温和灸通过调节miR-34a/sirt1/p53信号通路，对抗老年大鼠血管和肾脏衰老。”摘要：ObjectiveTo observe the effect of mild moxibustion on microRNA-34a （miR-34a）/silent information regulator 1 （sirt1）/tumor protein p53 （p53） signaling pathway in the thoracic aorta and kidney of senescent rats， so as to investigate the mechanism of mild moxibustion in attenuating vascular and renal aging.MethodsFifty 8-month-old male SD rats were given intraperitoneal injection of D-galactose （300 mg·kg-1·d-1） for 4 weeks to establish aging model. Twenty-four successfully modeled rats were randomly divided into aged control， medication and mild moxibustion groups （with 8 rats in each group）， with 8 additional 3-month-old male SD rats as the young control group. Rats of the mild moxibustion group received mild moxibustion at “Guanyuan” （CV4） and bilateral “Shenshu” （BL23） once daily for 5 d/week over 8 weeks. Rats of the medication group were intraperitoneally injected with testosterone propionate （7 mg/kg） every 3 d for 8 weeks. The aged and young control groups received equivalent volumes of 0.9% normal saline. Exhaustive swimming time was measured pre- and post-treatment. Histomorphology and collagen content of the thoracic aorta and kidney were assessed using HE and Masson staining. Serum total testosterone （TT） and free testosterone （FT） contents were detected by ELISA. Klotho protein expression in aortic and renal tissue was detected via immunohistochemistry. The relative protein expressions of sirt1， p53， and transforming growth factor-β1 （TGF-β1） were detected using Western blot， while real-time PCR was used to detect miR-34a， sirt1， and p53 mRNA expressions.ResultsCompared with the young control group， the exhaustive swimming time of rats in the aged control group shortened （P<0.01）， collagen deposition in aortic and renal tissues increased （P<0.01）， serum TT/FT content reduced （P<0.01）， the positive expression of Klotho， the protein and mRNA expression levels of sirt1 in aortic and renal tissue decreased （P<0.01）， and the expression levels of p53 protein and mRNA， TGF-β1 protein， and miR-34a mRNA increased （P<0.01）. Mild moxibustion and medication interventions reversed these trends： both groups showed prolonged swimming time （P<0.01）， reduced collagen （P<0.01）， elevated serum TT/FT content （P<0.01）， and down-regulated p53 protein， miR-34a mRNA in aortic and renal tissue （P<0.01）， TGF-β1 protein and p53 mRNA in aortic tissue （P<0.05，P<0.01）， and mRNA expression level of sirt1 in renal tissue （P<0.01）. Compared with the medication group， the collagen deposition of aortic tissue in the mild moxibustion group decreased （P<0.05）， positive expression of Klotho in renal tissue， and protein expression level of sirt1 in aortic tissue increased （P<0.05）， the protein expression level of p53 in renal tissue decreased （P<0.05）， while the mRNA expression levels of p53 in aortic tissue and miR-34a in renal tissue decreased （P<0.05）. HE staining showed thickened thoracic aorta and reduced glomeruli number in the aged control group， which was improved in the medication and mild moxibustion groups， including thinner thoracic aortic intima， more complete glomerular structure， and more regular renal icles and glomerular lumen， and the improvement in the mild moxibustion group was better than that in the medication group.ConclusionMild moxibustion exerts anti-aging effects on the thoracic aorta and kidney of senescent rats， which may be related with its effect on modulating the miR-34a/sirt1/p53 signaling pathway.关键词：Mild moxibustion;Aging;miR-34a/SIRT1/p53 signaling pathway30|9|0更新时间：2025-11-17
- “最新研究发现，电针能显著改善糖尿病大鼠坐骨神经功能，激活PPAR-γ信号通路，重塑糖脂代谢稳态，延缓糖尿病周围神经病变。”摘要：ObjectiveTo observe the protective effect of electroacupuncture（EA） on the structure and function of sciatic nerve in diabetic rats， so as to explore its mechanism in delaying diabetic peripheral neuropathy （DPN）.MethodsThirty SPF-grade male Sprague-Dawley （SD） rats were randomly divided into normal group， model group， and EA group （n=10）. Rats in the model group and EA group were intraperitoneally injected with streptozotocin （STZ， 60 mg/kg） to induce diabetes mellitus （DM） models. On the day after DM modeling， rats in the EA group received pre-intervention with EA （2 Hz， 10 min per session） at bilateral “Zusanli” （ST36）， “Sanyinjiao” （SP6）， “Pishu” （BL20）， and “Shenshu” （BL23） acupoints. 3 times a week for 8 weeks. Rats in the normal group and model group only received fixation treatment. Before intervention， and at the 4 and 8 week after intervention， body weight， random blood glucose， thermal withdrawal latency （TWL）， and mechanical withdrawal threshold （MWT） were measured. Following 8 weeks of intervention， the contents of total cholesterol （TC）， triglycerides （TG）， and non-esterified fatty acids （NEFA） in serum were determined using biochemical kits； the sensory nerve conduction velocity （SCV） and motor nerve conduction velocity （MCV） of the sciatic nerve were detected； the ultrastructural changes of the sciatic nerve were observed by transmission electron microscopy； transcriptome sequencing was used to screen differentially expressed genes （DEGs） for bioinformatics analysis； and qPCR was used to detect the mRNA expression levels of DEGs including adiponectin （Adipoq）， aquaporin 7 （Aqp7）， stearoyl-CoA desaturase 1 （Scd）， and perilipin 4 （Plin4）.ResultsCompared with the normal group， the model group showed significantly decreased body weight， TWL， MWT， SCV， and MCV （P<0.01）， significantly increased random blood glucose， serum TC， TG， and NEFA contents （P<0.01）， and accompanied by sciatic nerve ultrastructural damage （axon dispersion， myelin sheath separation）. After EA intervention， the above indicators and ultrastructural damage were significantly reversed （P<0.01）. A total of 186 DEGs were screened. Gene Ontology （GO） analysis showed that these DEGs were mainly involved in biological processes such as glucocorticoid response and fatty acid beta-oxidation. Kyoto Encyclopedia of Genes and Genomes （KEGG） analysis and gene set enrichment analysis （GSEA） indicated that the DEGs were significantly enriched in the peroxisome proliferator-activated receptor （PPAR） signaling pathway， which changed from an inhibited state to an activated state after EA intervention. Through the identification of core pathway genes， the PPAR-γ pathway and its downstream target genes （Adipoq， Aqp7， Scd， Plin4） were finally identified as key regulatory targets. qPCR verification showed that compared with the normal group， the mRNA expressions of Adipoq， Aqp7， Scd， Plin4 in the model group were significantly down-regulated （P<0.01）， while their expressions were significantly reversed after EA intervention （P<0.01）， and this change trend was consistent with the results of transcriptome sequencing.ConclusionEA may activate the PPAR-γ signaling pathway， synergistically up-regulate the mRNA expressions of Adipoq， Aqp7， Scd， and Plin4， thereby remodeling the homeostasis of glucose and lipid metabolism， significantly improving the structure and function of the sciatic nerve， and delaying the progression of DM to DPN.关键词：Electroacupuncture;Diabetic peripheral neuropathy;Transcriptomics;Peroxisome proliferator-activated receptor γ signaling pathway24|19|0更新时间：2025-11-17
- “在卒中后吞咽障碍治疗领域，研究显示头针结合颈前五穴治疗能显著改善患者吞咽功能和生活质量，具有良好安全性。”摘要：ObjectiveTo observe the effects on swallowing reflex latency （SRL）， the distances of hyoid bone forward and upward movements in the patients with post-stroke dysphagia （PSD） based on videofluoroscopic swallow study （VFSS） after treated with scalp acupuncture combined with five points in the anterior cervical region.MethodsSixty-six patients with PSD were randomly divided into a trial group （33 cases， 1 case dropped out） and a control group （33 cases， 1 case dropped out）. The basic treatment with neurological medicine and with acupuncture for stroke （Neiguan ［PC6］， Sanyinjiao ［SP6］， Chize ［LU5］ and Weizhong ［BL40］ on the affected side） were provided in the two groups. Besides， in the trial group， scalp acupuncture was delivered and combined with five points in the anterior cervical region； and in the control group， acupuncture was applied to Shuigou （GV26）， Lianquan （CV23）， Tongli （HT5） and Zhaohai （KI6）. The treatment was administered once daily， for 30 min each time； and 2 weeks of treatment were required， with the interval of 1 d after 6 interventions. Using VFSS， SRL and the distances of hyoid bone forward and upward movements were assessed before and after treatment in the 2 groups； and the changes in the scores of standardized swallowing assessment （SSA）， water swallowing test （WST）， and swallowing quality of life questionnaire （SWAL-QOL） were observed. The correlation was analyzed between VFSS indicators and scale assessment results.ResultsCompared with those before treatment， SRL was shortened （P<0.01）， the distances of hyoid bone forward and upward movements increased （P<0.01） in both groups. When compared with the control group after treatment， SRL was shorter （P<0.01）， the distances of hyoid bone forward and upward movements were longer （P<0.01） in the trial group. SSA score was reduced in comparison with that before treatment in either group （P<0.01）， and the score in the trial group was lower than that of the control group （P<0.01）. The WST grade was better after treatment in both groups （P<0.01）， and the grade in the trial group was superior to that in the control group （P<0.05）. SWAL-QOL score was increased after treatment in both groups （P<0.01）， and the score in the trial group was higher than that of the control group （P<0.01）. The clinical effective rate was 93.75% （30/32） in the trial group， which was higher than that （81.25%， 26/32） in the control group （P<0.05）. The correlation analysis showed that the SSA score and WST grade were positively correlated with SRL before and after treatment （P<0.01）， and were negatively correlated with the distances of hyoid bone forward and upward movements （P<0.01）. During trial， no obvious adverse reactions occurred.ConclusionAcupuncture at head points， combined with the five points in the anterior cervical region significantly shortens SRL， increases the distances of hyoid bone forward and upward movements and effectively improves swallowing function and quality of life in the patients. SRL and the distances of hyoid bone forward and upward movements can be taken as the objective indicators to assess dysphagia in clinic. Scalp acupuncture combined with the five points in the anterior cervical region is effective and safe on PSD.关键词：Post-stroke dysphagia;Scalp acupuncture;Five points in the anterior cervical region;Videofluoroscopic swallow study36|55|0更新时间：2025-11-17
- “在脾虚湿阻型腹泻型肠易激综合征治疗领域，专家验证了穴位贴敷神阙的疗效，为提高患者生活质量提供新方案。”摘要：ObjectiveTo observe the clinical efficacy of acupoint application in the treatment of diarrhea type irritable bowel syndrome （IBS-D） with spleen deficiency and dampness retention.Methods88 patients with spleen deficiency and dampness retention type IBS-D were randomly divided into a control group （44 cases， 1 case dropped out， 1 exclusion） and an experimental group （44 cases， 2 cases dropped out）. The control group was given Bifidobacterium triple active bacteria capsules （Bifico） orally， 0.48 g each time， twice a day. Patients in the experimental group applied joint acupoint plaster to Shenque （CV8） in addition to the control group for 12 h each time， once a day. Both groups underwent treatment for 7 d per course， totaling 4 courses. The traditional Chinese medicine syndrome score， IBS severity scale （IBS-SSS） score， and IBS stool characteristics questionnaire （IBS-DSQ） score of the 2 groups were recorded before and after 4 weeks of treatment， and the clinical efficacy of the 2 groups were observed.ResultsAfter treatment， the TCM syndrome score， IBS-SSS score， and IBS-DSQ score of both groups of patients was decreased compared to those before treatment （P<0.001）， and the experimental group was significantly better than the control group （P<0.01， P<0.001， P<0.05）； the symptom scores of diarrhea， abdominal pain， and abdominal fullness in the experimental group were significantly lower than those in the control group （P<0.01， P<0.05）， while there were no statistically significant difference in the scores of fatigue and drowsiness， and anorexia between the two groups. The total effective rate of the experimental group was 88.1% （37/42）， which was higher than the control group’s 73.8% （31/42）， and the difference was statistically significant （P<0.05）.ConclusionAcupoint application at CV8 can effectively improve diarrhea， abdominal pain， abdominal distension， and stool characteristics in patients with spleen deficiency and dampness retention type IBS-D， and improve their quality of life. The efficacy is definite， safe， and reliable， and it is worthy of further clinical promotion and application.关键词：Acupoint application;Diarrhea-predominant irritable bowel syndrome;Randomized controlled trial47|64|0更新时间：2025-11-17
- “最新研究显示，隔药饼灸联合阿托伐他汀钙治疗痰瘀互结型颈动脉粥样硬化患者，疗效显著且安全。”摘要：ObjectiveTo observe the clinical effect and safety of herbal cake-insulated moxibustion in the auxiliary treatment of carotid atherosclerosis （CAS） differentiated as interaction of phlegm and blood stasis.MethodsSixty-three patients with CAS differentiated as interaction of phlegm and blood stasis were randomly divided into an experimental group （33 cases， 4 cases dropped out） and a control group （30 cases， 3 cases dropped out）. In the experimental group， herbal cake-insulated moxibustion was delivered in combination with oral administration with atorvastatin. In the control group， atorvastatin was administered orally. The duration of treatment in either group was composed of 12 weeks. Common carotid intima-media thickness （cIMT）， the total area of carotid plaque， common carotid blood flow parameters， blood lipids contents （4 items） and traditional Chinese medicine （TCM） syndrome score were compared between the two groups before and after treatment. Blood routine and liver and kidney function were detected before and after treatment， and adverse events were recorded to evaluate safety.ResultsCompared with those before treatment， the bilateral cIMT and the contents of total cholesterol （TC）， triglyceride （TG） and low-density lipoprotein cholesterol （LDL-C） in serum were reduced after treatment （P<0.05， P<0.001）， the scores of the primary symptoms and secondary symptoms of TCM， as well as the total score were lower （P<0.001） in the two groups. The left cIMT and the contents of the serum TC， TG and LDL-C in the experimental group were lower than those of the control group （P<0.05， P<0.01）， and the scores of the primary symptoms and secondary symptoms of TCM， as well as the total score were lower than those of the control group （P<0.001， P<0.01）. After treatment， the bilateral peak systolic velocity （PSV） and end diastolic velocity （EDV） were increased in the experimental group （P<0.001）， the right EDV was increased in the control group （P<0.001）， and the bilateral PSV and EDV in the experimental group were higher than those of the control group （P<0.05， P<0.01， P<0.001）. After treatment， there was no significant difference in the total area of carotid plaque between the two groups. After treatment， there were no significant abnormalities in blood routine and liver and kidney functions in both groups， and the safety was good.ConclusionOn the basis of conventional medication， the herbal cake-insulated moxibustion as an auxiliary treatment， presents its synergistic effect on CAS of interaction of phlegm and blood stasis syndrome in the patients， and this therapy is safe in clinical application.关键词：Herbal cake-insulated moxibustion;Atherosclerosis;Carotid artery ultrasound;Interaction of phlegm and blood stasis;Randomized controlled trial40|28|0更新时间：2025-11-17
- “针灸在急性肺损伤治疗中通过调控促炎信号通路抑制过度炎性反应，为抗炎机制研究及推广应用提供科学依据。”摘要：Acute lung injury （ALI） is a severe and critical condition， characterized by high incidence and mortality rates， posing significant challenges to current treatment strategies. In recent years， the application of acupuncture in treating ALI has attracted widespread attention. This paper focuses on the role of acupuncture in treating ALI and systematically summarizes the mechanism by which acupuncture inhibits excessive inflammatory responses and maintains immune homeostasis through the regulation of pro-inflammatory signaling pathways such as mitogen-activated protein kinase （MAPK）， nuclear factor kappa B （NF-κB）， phosphatidylinositol 3-kinase / protein kinase B （PI3K/Akt）， janus kinase / signal transducer and activator of transcription （JAK/STAT）. Furthermore， it explores the specific role of acupuncture in reducing lung inflammation and tissue damage， providing a scientific basis and reference for further study of its anti-inflammatory mechanism and application in ALI.关键词：Acupuncture;Acute lung injury;Inflammatory response;Signaling pathway;Review64|35|0更新时间：2025-11-17
- “最新研究扩充大鼠腧穴定位数据库，绘制实体及三维腧穴图谱，推进实验针灸学研究标准化进程。”摘要：ObjectiveTo expand the existing database of point location in experimental rats based on literature research， and draw the body and three-dimensional atlas of points in rats， so as to provide a visual reference of the points and meridians for experimental acupuncture study in rats.MethodsThe research was composed of three parts： literature investigation， drawing rat point atlas on the physical body， and constructing rat three-dimensional point atlas. The methods of locating points in rats were screened and sorted out by searching relevant literature in China national knowledge infrastructure database， Wanfang database and PubMed. Combined with the anatomical characteristics of rats， the body proportion measurement and body surface anatomy were used to mark the points on rat body， and draw the rat point atlas on the physical body. Using Vet-anatomy， Adobe Illustrator， Goodnotes and other softwares for image processing， the three-dimensional point atlas and a part of the meridian course map of rats were developed.ResultsA total of 148 rat points were identified based on literature research， of which， 124 points were located according to body surface anatomy， 15 points based on body proportional measurement and 9 points in terms of the above two methods combined. The points were mostly identified from the Bladder Meridian， followed by the Governor Vessel， the Conception Vessel and the Stomach Meridian. The point atlas on the real rat body was developed， the three-dimensional atlas of rat points， and the running courses of a part of meridians were constructed. The locating methods and descriptions of all 148 points were described and summarized in a table.ConclusionThis study provides the map of 148 acupoints in rats and the running courses of some meridians， which is conducive to promoting the study on the standardization of rat points and meridians.关键词：Rat;Acupoint location;Meridian courses;Atlas;Anatomy34|12|0更新时间：2025-11-17
- 摘要：ObjectiveTo assess the efficacy and safety of scalp acupuncture in the treatment of autism spectrum disorder （ASD）.MethodsA comprehensive search was conducted using PubMed， EMbase， The Cochrane Library， Web of Science， SinoMed， China National Knowledge Internet， China Science and Technology Journal Database， and Wanfang Data databases. The Cochrane Handbook of Systematic Reviews 5.1.0 was used to evaluate the risk of bias in the included randomized control trials （RCTs）. A Meta-analysis was performed using RevMan 5.4.1 statistical software.ResultsA total of 33 RCTs were included， including 2 701 patients with ASD. Meta-analysis results showed that compared with the rehabilitation training group， the scalp acupuncture combined with rehabilitation training could significantly reduce the autism behavior rating scale score ［MD= -5.70， 95% CI （-6.51， -4.89）， P<0.01］， and childhood autism rating scale score ［MD= -4.02， 95% CI （-4.38， -3.66）， P<0.01］， and improve the Gesell developmental diagnostic scale-social adaptative developmental quotient ［MD=5.90， 95% CI （4.29， 7.51）， P<0.01］， Gesell-language development quotient ［MD=4.39， 95% CI （3.03， 5.75）， P<0.01］， Gesell-personal social competence ［MD=4.32， 95% CI （2.48， 6.15）， P<0.01］， psychoeducational assessment for children 3rd edition （PEP-3）-communication skills score ［MD=3.76， 95% CI （3.25， 4.27）， P<0.01］， PEP-3-physical agility score ［MD=1.99， 95% CI （1.40， 2.59）， P<0.01］， and PEP-3-behavior score ［MD=2.18， 95% CI （1.77， 2.59）， P<0.01］.ConclusionScalp acupuncture is effective in improving the language problems， behavior problems， social adaptation and other symptoms of autism children， and has high safety. However， due to the insufficient quality of the research methods included in the literature， the integration of subjects with different severity and age， and the wide variation in the duration of the intervention， the conclusion of this study still needs to be validated by more rigorous and high-quality randomized controlled trials， with long-term follow up.关键词：Scalp acupuncture;Autism spectrum disorder;Meta-analysis;Systematic review;Randomized controlled trial55|42|0更新时间：2025-11-17
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