最新刊期
卷 50 ， 期 12 ， 2025
- “最新研究发现，1 mA、2 mA强度电针及相同频率手针对急性炎性内脏痛有类似镇痛效果，且优于高强度电针；其作用机制可能与α7nAChR/PI3K/AKT信号通路密切相关。”摘要：ObjectiveTo compare the effects of electroacupuncture （EA） and manual acupuncture （MA） at different stimulation intensities on acute inflammatory visceral pain， and to analyze their mechanisms underlying relief of inflammatory visceral pain mediated by α7 nicotinic acetylcholine receptor （α7nAChR）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） signaling pathway.MethodsMale SD rats were randomly divided into normal control， visceral pain model， EA 1 mA， EA 2 mA， EA 3 mA， EA 4 mA， EA 6 mA， and MA groups （with the same frequency as EA， 2 times/s， for 2 min）， with 12 rats in each group. The acute inflammatory visceral pain model was established by intracolonic instillation of 2% acetic acid. EA （2 Hz， 1， 2， 3， 4， 6 mA） or MA （120 twirlings/min， with the needle rotated at a magnitude of about 90°—180°） was applied to the right “Shangjuxu” （ST37） for 2 min. The abdominal withdrawal reflex （AWR） score and external oblique muscle electromyogram amplitude were used to assess the visceral pain reaction. Histopathological changes of the colonic mucosal tissue were detected by H.E. staining. For observing the involvement of colonic α7nAChR in visceral pain relief of both EA and MA， intraperitoneal injection of methylaconitine （MLA， α7nAChR blocker， 4 mg/kg） was given to rats of MA+MLA and EA 2 mA+MLA groups （with 6 rats in each group） before each intervention. The contents of serum tumor necrosis factor-α（TNF-ɑ） and interleukin-1β（IL-1β） were detected by enzyme-linked immunosorbent assay （ELISA）. The expression levels of α7nAChR， PI3K and AKT in the colon tissue were detected by Western blot.ResultsIn comparison with the normal control group， both the AWR scores and the amplitude of EMG at colorectal dilation （CRD） pressure of 20， 40， 60 and 80 mmHg were significantly increased in the model group （P<0.05）. Compared with the model group， both the AWR scores and the amplitude of EMG at CRD pressure of 20， 40， 60 and 80 mmHg were significantly decreased in the EA 1 mA， EA 2 mA and MA groups （P<0.05）， rather than in the EA 3 mA， EA 4 mA and EA 6 mA groups， suggesting that the higher stimulation intensity of EA had no analgesic effect. H.E. staining showed slightly hyperemic and edematous injury of the colonic mucosa tissue， with obvious inflammatory infiltration and abnormal morphological changes in the model group， which was obviously milder in the injury in the MA and EA 1 mA， EA 2 mA groups， rather than in the EA 3 mA， 4 mA and 6 mA groups. In comparison with the normal control group， the contents of serum TNF-ɑ and IL-1β， and expression levels of PI3K and AKT proteins in colon were significantly increased （P<0.05）， and the expression of α7nAChR protein was remarkably down-regulated （P<0.05） in the model group. Compared with the model group， the contents of serum TNF-ɑ and IL-1β， and the expression levels of PI3K and AKT proteins were strikingly down-regulated （P<0.05）， and the expression of α7nAChR was considerably up-regulated （P<0.05） in both EA 2 mA and MA groups. Moreover， the effects of reducing the corresponding AWR scores at various CRD pressure levels， serum TNF-α and IL-1β contents， and the protein expressions of PI3K and AKT in colon， as well as increasing the protein expression of α7nAChR in colon in rats of MA and EA 2 mA groups can be inhibited by MLA （P<0.05）， a blocker of α7nAChR.ConclusionBoth MA and 2 mA EA （not 3， 4， 6 mA EA） stimulation of ST37 have a similar analgesic effect in acute inflammatory visceral pain rats， which may be related to their function in suppressing the activities of α7nAChR/PI3K/AKT signaling pathway.关键词：Manual acupuncture;Electroacupuncture;Acute inflammatory visceral pain;α7nAChR/PI3K/AKT signaling pathway;Shangjuxu（ST37）38|20|0更新时间：2025-12-22
- “最新研究发现，“疏肝调神”针刺可改善慢性炎性痛与抑郁共病大鼠海马神经元损伤，促进神经修复与重塑。”摘要：ObjectiveTo observe the effect of “Shugan Tiaoshen” （regulating mental activities by relieving depressed liver） acupuncture on hippocampal iron metabolism， antioxidant system and lipid peroxidation related factors in chronic inflammatory pain and depression comorbidity （CIPDC） rats and its protective effect on hippocampal neurons， so as to explore its mechanisms underlying improvement of CIPDC.MethodsA total of 48 male SD rats were randomly divided into control， model， electroacupuncture （EA） and medication （paroxetine） groups， with 12 rats in each group. The CIPDC model was established by injection of complete Freund’s adjuvant into the toe surface of the left hind paw. From 15 to 28 days after modeling， EA was applied to “Yintang” （GV24+）， “Baihui” （GV20）， “Hegu” （LI4） and “Taichong” （LR3） for 30 min， once a day for 14 days. Rats in the medication group were treated with intragastric administration of paroxetine （10 mg/kg）， once a day for 14 days. Paw withdrawal mechanical threshold （PWMT） and paw thermal withdrawal latency （PTWL） were detected for evaluating the rats’ pain response behavior in each group at 0， 7， 14， 21 and 28 days after modeling. The sucrose preference test， open field test （OFT） and forced swimming test （FST） were conducted to calculate the sucrose preference value， and to record the total distance traveled in 30 min and the time spent in the central zone of the open field during the first 5 min， and the immobility （floating） time during the last 4 min for evaluating the rats’ depression-like behavior at 0， 14 and 28 days after modeling. H.E. staining was used to observe the histopathological changes of hippocampal neurons. Prussian blue staining was used to observe the iron deposition in the hippocampus tissue. A transmission electron microscopy was used to observe the ultrastructure of mitochondria of hippocampal neurons. The content of Fe2+ in the hippocampus tissue was detected by colorimetry method. ELISA method was used to measure the contents of glutathione （GSH） and malondialdehyde （MDA） in the hippocampus tissue. The expression levels of ferritin heavy chain 1 （FTH1）， ferritin light chain （FTL）， solute carrier family 7 member 11 （SLC7A11）， glutathione peroxidase 4 （GPX4）， acyl-coa synthetase long chain family member 4 （ACSL4）， and lipoxygenase （LOX） proteins and mRNAs of hippocampus were detected by Western blot and real-time fluorescence quantitative PCR， separately.ResultsCompared with the control group， the PWMT and PTWL at 7， 14， 21 and 28 days after modeling， sucrose preference value （SPV）， total motion distance of OFT， time spent in the central zone， GSH content， and expression levels of FTH1， FTL， SLC7A11 and GPX4 proteins and mRNAs were significantly decreased （P<0.01）， while immobility time of FST， the contents of Fe2+ and MDA， and the expression levels of ACSL4 and LOX protein and mRNAs were considerably increased （P<0.01） in the model group. In contrast to the model group， both EA and medication group had an obvious increase in the PWMT and PTWL at 21 and 28 days after modeling， SPV， total motion distance， time spent in the central zone， GSH content， and the expression levels of FTH1， FTL， SLC7A11 and GPX4 proteins and mRNAs （P<0.01， P<0.05）， and a striking decrease in the immobility time， contents of Fe2+ and MDA， and the expression levels of ACSL4 and LOX protein and mRNAs （P<0.01， P<0.05）. No significant differences were found between the EA and medication groups in all the indexes mentioned above. Morphological observation showed a large number of yellow-brown iron deposition in the hippocampus， neuronal degeneration， disordered arrangement and necrosis of neurons， shrinkage and deformation of mitochondria， increased membrane density， blurred boundary， and decrease and disappearance of mitochondrial cribriae in the model group， which was obviously milder in the injury state in both EA and medication groups， including reduction of yellow-brown iron deposition， regular arrangement of neurons， obvious nucleolus and complete structure of mitochondria， etc.Conclusion“Shugan Tiaoshen” acupuncture can improve the inflammatory pain and depression in rats with CIPDC ， which may be related to its functions in activating antioxidant activity and promoting iron metabolism balance， reducing lipid peroxidation， and inhibiting ferroptosis， thereby promoting the repair and remodeling of hippocampal neurons and ameliorating the disease process of CIPDC.关键词：Chronic inflammatory pain;Depression;Comorbidity;“Shugan Tiaoshen” acupuncture;Ferroptosis;Hippocampal neurons37|204|0更新时间：2025-12-22
- “最新研究发现，通督醒神针刺能通过调节HIF-1α/PDK1信号通路，改善脑缺血再灌注大鼠学习记忆障碍。”摘要：ObjectiveTo observe the effect of Tongdu Xingshen （unblocking the Governor Vessel and refreshing the spirit） needling on hypoxia-induced factor-1α/pyruvate dehydrogenase kinase 1 （HIF-1α/PDK1） pathway and hippocampal glycolysis level in rats with cerebral ischemia-reperfusion injury （CIRI）， so as to explore its mechanisms underlying improvement of learning and memory ability.MethodsSD rats were randomly divided into sham operation， model， acupuncture and acupuncture + inhibitor groups （n=12 in each group）. The CIRI model was established by middle cerebral artery occlusion/reperfusion （MCAO/R）. EA （1 Hz/20 Hz） was applied to “Shenting” （GV24） and “Baihui” （GV20） for 30 min， once daily for 14 consecutive days. In the acupuncture + inhibitor group， 2ME2 （5 mg/kg） was injected intraperitoneally 30 min before modeling. Learning and memory ability were evaluated by using open field test and new object recognition experiment. TTC staining was used to detect cerebral infarction volume. The pathological changes of hippocampus were observed by HE staining. The contents of lactic acid and ATP in hippocampal tissue were detected by colorimetric assay kit. The protein and mRNA expression levels of HIF-1α， PDK1， hexokinase 2 （HK2）， phosphofructokinase 1 （PFK1） and pyruvate kinase M2 （PKM2） in hippocampal tissues on the ischemic side were detected by Western blot and qPCR， respectively.ResultsCompared with the sham operation group， the rats in the model group had a significant decrease in the proportion of central distance， the number of crossing platform and the new object preference index （P<0.01）， and a significant increase in percentage of cerebral infarction volume （P<0.01）. The content of lactic acid in hippocampus， the protein and mRNA expression of HIF-1α， PDK1， HK2， PFK1 and PKM2 were increased significantly （P<0.01）， while the content of ATP was decreased （P<0.01） in the model group compared with those in the sham operation group. Following the interventions， modeling induced increase and decrease of the indexes mentioned above were all reversed in the acupuncture group （P<0.01）， and the therapeutic effects of acupuncture + inhibitor were better than those of acupuncture alone （P<0.01， P<0.05）. H.E. staining showed disordered cell arrangement， abnormal morphology， and evident nuclear rupture and nuclear pyknosis in the hippocampus tissue in the model group， which was relatively milder in the two intervention groups.ConclusionTongdu Xingshen needling can improve the learning and memory function of CIRI rats， which may be associated with its function in down-regulating the expression of HIF-1α/PDK1 signaling pathway， inhibiting anaerobic glycolysis in the hippocampus， reducing lactic acid accumulation and increasing ATP production.关键词：Post-stroke cognitive impairment;Electroacupuncture;Glycolysis;HIF-1α/PDK1 signaling pathway55|234|0更新时间：2025-12-22
- “最新研究发现，针刺能减少脑缺血再灌注损伤大鼠脑梗死体积，改善神经功能缺损症状，可能通过抗铜死亡发挥作用。”摘要：ObjectiveTo investigate the effect of acupuncture on the expressions of cuproptosis marker proteins-ferredoxin 1 （FDX1） and lipoic acid synthase （LIAS） in the ischemic penumbra （IP） of rats with cerebral ischemia-reperfusion injury （CIRI）.MethodsSD rats were randomly divided into sham operation， model and acupuncture groups （n=15）. The CIRI model was established by occlusion of the middle cerebral artery and reperfusion （MCAO/R）. In the acupuncture group， “Dazhui” （GV14）， “Baihui” （GV20） and “Shuigou” （GV26） were punctured with filiform needles for 30 min， once every 12 h for 7 times. Neurological function was assessed using modified neurological severity scores （mNSS）. Cerebral infarction volume was measured by TTC staining. Copper ion content in IP tissue was determined by colorimetric method. Mitochondrial respiratory chain complexes （MRCC）Ⅰ and Ⅱ contents were detected by ELISA. Protein expressions of FDX1， LIAS， and heat shock protein 70 （HSP70） were analyzed by Western blot. Mitochondrial morphology and structure in the IP region were observed via transmission electron microscopy （TEM）.ResultsCompared with the sham operation group， the mNSS score （P<0.01）， the volume percentage of cerebral infarction （P<0.000 1）， copper ion in IP （P<0.01）， MRCCⅠ and Ⅱ contents （P<0.000 1） and HSP70 protein expression （P<0.001） were significantly increased， while the expression of FDX1 （P<0.01） and LIAS （P<0.001） were decreased in the model group. Compared with the model group， the mNSS score （P<0.05）， the volume percentage of cerebral infarction （P<0.001）， the contents of IP copper ion （P<0.001）， MRCCⅠ and Ⅱ contents （P<0.01，P<0.000 1） and HSP70 protein expression decreased （P<0.01）， whereas the protein expressions of FDX1 and LIAS were increased （P<0.05） in the acupuncture group. TEM results showed that the outer membrane of mitochondria swelled and ruptured， the inner cristae ruptured and disappeared， and the density of the mitochondrial matrix decreased in the model group， which were relatively milder in the acupuncture group.ConclusionAcupuncture intervention can reduce the volume of cerebral infarction and improve the symptoms of neurological deficit in CIRI rats， and its mechanism may be related to reducing the loss of FDX1 and LIAS proteins and inhibiting the surge of HSP70 protein， thus preventing cuproptosis in brain cells.关键词：Acupuncture;Cerebral ischemia-reperfusion injury;Cuproptosis;FDX1;LIAS33|75|0更新时间：2025-12-22
- “最新研究发现，电针特定穴位能改善帕金森病模型小鼠运动功能，提高中脑黑质酪氨酸羟化酶表达，可能通过调控IGF-1R/IRS-1/PI3K/AKT信号通路抑制神经炎性反应。”摘要：ObjectiveTo observe the effect of electroacupuncture（EA） at “Fengfu” （GV16）， “Taichong” （LR3） and “Zusanli” （ST36） on the behavior and insulin receptor substrate 1 （IRS-1）/phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） pathway mediated by the insulin-like growth factor 1 receptor （IGF-1R） in the substantia nigra of the midbrain in mice model with Parkinson’s disease （PD）， so as to explore the mechanism of EA in the prevention and treatment of PD.MethodsC57BL/6 mice were randomly divided into a control group， a model group， and an EA group， with 10 mice in each group. A PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine （MPTP， 30 mg·kg-1·d-1） for 7 consecutive days. The mice in the EA group were given EA at GV16， LR3， and ST36 for 30 min each time， once a day for 12 consecutive days. The open field test was used to observe the behavioral changes of the mice in each group before and after treatment. Immunohistochemistry was used to detect the expression level of tyrosine hydroxylase （TH） in the substantia nigra region of the midbrain. Real-time fluorescence quantitative PCR and Western blot were used to detect the mRNA expression levels of IGF-1R， IRS-1， PI3K， and AKT， and protein expressions of IGF-1R， IRS-1， p-PI3K， and p-AKT in the substantia nigra of the mice， respectively. ELISA was used to detect the contents of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in the substantia nigra of the midbrain.ResultsCompared with the control group， mice in the model group showed obvious motor dysfunction. The total distance of spontaneous movement， the average speed， and the duration of movement， the positive expression level of TH in the substantia nigra of the midbrain， and the mRNA expression levels of IGF-1R， PI3K， and AKT， and protein expressions of IGF-1R， p-PI3K/PI3K and p-AKT/AKT ratio in the substantia nigra decreased （P<0.01， P<0.05）， while the mRNA and protein expression levels of IRS-1， the contents of the inflammatory factors TNF-α and IL-1β increased （P<0.05， P<0.01）. Compared with the model group， all the above indexes of the PD mice after EA treatment were all reversed （P<0.01， P<0.05）.ConclusionEA at GV16， LR3， and ST36 can effectively improve the bradykinesia of PD model mice induced by MPTP， increase the expression level of TH in the substantia nigra， and improve the insulin signaling dysfunction. Its mechanism may be related to the regulation of the IGF-1R/IRS-1/PI3K/AKT signaling pathway and the subsequent inhibition of the neuroinflammatory response.关键词：Parkinson’s disease;Electroacupuncture;Neuroinflammatory response;Insulin-like growth factor 1 receptor;Phosphatidylinositol 3-kinase/protein kinase B36|238|0更新时间：2025-12-22
- “最新研究发现，电针治疗能改善2型糖尿病大鼠胰岛素抵抗，减轻骨骼肌脂质堆积，降低炎症反应，作用机制与调节PPAR-γ/NF-κB信号通路有关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Sanyinjiao” （SP6） and “Zusanli” （ST36） on the expressions of peroxisome proliferator-activated γ receptor （PPAR-γ）， nuclear factor-κB （NF-κB） and glucose transporter 4 （GLUT4） in skeletal muscle of Zucker Diabetic Fatty （ZDF） rats with type 2 diabetes mellitus （T2DM）， so as to explore its possible mechanism in improving insulin resistance （IR）.MethodsEighteen male 2-month-old ZDF （Leprfa/fa） rats were fed with a high-fat diet for 4 weeks to establish a diabetic model. After the model was confirmed， the rats were randomly divided into model， EA and medication groups， with 6 rats in each group. Additionally， 6 Zucker lean rats （Lepr+/fa） of the same age were used as the control group. EA （15 Hz， 1 mA） was applied to bilateral SP6 and ST36 for 20 min； the medication group was administered pioglitazone solution （10 mg/kg） via gastric gavage； both groups were treated once daily， 5 d a week for 4 weeks. Fasting body weight （FBW） was measured weekly， fasting blood glucose （FBG） was measured the day before sampling. The levels of serum fasting insulin （FINS）， free fatty acid （FFA）， low density lipoprotein （LDL）， total cholesterol （TC）， triglyceride （TG）， tumor necrosis factor-α （TNF-α）， C peptide and C reactive protein （CRP） were detected by ELISA， and the homeostasis model assessment of insulin resistance （HOMA-IR） index was calculated. HE staining and oil red O staining were used to observe the pathological changes and lipid droplet accumulation of skeletal muscle in rats respectively. The protein expression levels of PPAR-γ， NF-κB and phosphorylated （p） -NF-κB in skeletal muscle tissue of rats were detected by Western blot. The positive expressions of TNF-α and GLUT4 in skeletal muscle of rats were detected by immunofluorescence.ResultsCompared with the control group， the FBW， FBG， HOMA-IR index， the serum levels of FINS， FFA， LDL， TG， TC， TNF-α， C-peptide and CRP， NF-κB phosphorylation level， as well as the positive expression of TNF-α and p-NF-κB/NF-κB in skeletal muscle were increased （P<0.01）， while the PPAR-γ expression and the positive expression of GLUT4 in skeletal muscle were decreased （P<0.01） in the model group. Oil red O staining showed a large number of bright red lipid droplets and serious lipid accumulation in skeletal muscle cells， HE staining showed that the skeletal muscle fibers were broken in the model group. Compared with the model group， the FBW of the medication group， the expression levels of PPAR-γ and GLUT4 of both EA and medication groups were increased （P<0.01）. Conversely， the levels of FBG， HOMA-IR index， FINS， FFA， LDL， TG， TC， TNF-α， C-peptide and CRP， the phosphorylation level of NF-κB protein and TNF-α expression were decreased （P<0.05， P<0.01） in the EA and medication groups. The oil red O staining showed that the lipid droplets in the skeletal muscle cells were significantly reduced， and the degree of lipid accumulation was alleviated， whereas HE staining showed reduced skeletal-muscle fiber disruption in the EA and medication groups compared to those in the model group.ConclusionEA can ameliorate energy metabolism disorders in T2DM rats， reduce ectopic lipid accumulation in skeletal muscle， and alleviate inflammatory responses， which may be related to the regulation of the PPAR-γ/NF-κB signaling pathway.关键词：Type 2 diabetes mellitus;Insulin resistance;Electroacupuncture;Inflammation;PPAR-γ;NF-κB30|31|0更新时间：2025-12-22
- “最新研究发现，针刺能通过抑制TGF-β/Smad3信号通路，改善肥胖大鼠代谢状态，减小脂肪细胞体积，实现减重效应。”摘要：ObjectiveTo observe the effect of acupuncture on the expressions of transforming growth factor β（TGF-β）/Smad homolog 3（Smad 3）signaling pathway and autophagy-related factors in obese rats induced by a high-fat diet，so as to explore its possible mechanisms in improving obesity.MethodsMale SD rats were randomly divided into normal，model，medication and acupuncture groups，with 10 rats in each group.The obesity model was established by feeding the rats with a high-fat diet.Rats of the acupuncture group received acupuncture at “Guanyuan”（CV4） “Zhongwan”（CV12） and bilateral “Tianshu”（ST25） etc.， for 30 min， once daily for 4 weeks. Rats in the medication group received gavage of orlistat solution （32.4 mg·kg-1·d-1）， once daily for 4 weeks.The rat’s body weight was measured for calculating the Lee’s index.The weights of epididymal fat and perirenal fat were measured.The fasting blood glucose （FBG） and insulin levels were measured， and the homeostasis model assessment of insulin resistance （HOMA-IR） index was calculated. Additionally， intraperitoneal glucose tolerance and insulin tolerance tests were performed.The serum levels of triglyceride （TG）， total cholesterol （TC）， low-density lipoprotein （LDL） and high-density lipoprotein （HDL） were measured. HE staining was used to observe the morphology and structure of liver and epididymal adipose tissue， and oil red O staining was used to observe the lipid droplets in liver tissue.The mRNA and protein expression levels of TGF-β， TGF-β receptor 1 （TGF-βR1）， Smad3， autophagy related protein Beclin-1， ubiquitin binding protein p62， and microtubule-associated protein 1 light chain 3（LC3） in epididymal adipose tissue were detected by real-time fluorescence quantitative PCR and Western blot， respectively.ResultsCompared with the normal group， the body weight， Lee’s index， perirenal and epididymal fat mass，serum TC， TG and LDL content， fasting insulin level， and HOMA-IR of rats in the model group were increased （P<0.01）， the blood glucose levels were also increased at 30， 60， and 120 min after glucose and insulin injection （P<0.01，P<0.05），while the HDL content was decreased （P<0.01）. Compared with the model group， the body weight， Lee’s index， perirenal and epididymal fat mass，serum TC， TG and LDL content， and HOMA-IR of the medication and acupuncture groups were decreased （P<0.01，P<0.05）， and the serum HDL content of the acupuncture group was increased （P<0.01）. Compared with the normal group， the mRNA expression levels of TGF-β， TGF-βR1， Smad3， LC3， and Beclin-1， the protein expression levels of TGF-β， TGF-βR1， p-Smad3/Smad3， LC3-Ⅱ/LC3- Ⅰ， and Beclin-1 in the epididymal adipose tissue of rats in the model group were significantly increased （P<0.01，P<0.05），while the mRNA and protein expression levels of p62 were decreased （P<0.01）. Compared with the model group， the mRNA expression levels of TGF-β， LC3， and Beclin-1， the protein expression levels of TGF-β， p-Smad3/Smad3， and Beclin-1 in the medication and acupuncture groups were decreased （P<0.01），while the mRNA and protein expression levels of p62 were increased （P<0.01）. Additionally， the mRNA expression of TGF-βR1， the protein expression of TGF-βR1 and LC3-Ⅱ/LC3- Ⅰ in the acupuncture group was decreased （P<0.05，P<0.01）. Compared with the medication group， the serum HDL content and the expression of p62 protein in adipose tissue were significantly increased in the acupuncture group （P<0.01）， while the protein expression level of TGF-βR1， the ratio of p-Smad3/Smad3， and the expression of Beclin-1 protein in adipose tissue were significantly decreased （P<0.01）. Compared with the normal group， the adipocytes in the model group exhibited significantly enlarged lipid droplets with disordered arrangement， and hepatic lipid deposition was markedly increased； whereas compared with the model group， both the acupuncture and medication groups showed reduced lipid droplet size.ConclusionAcupuncture may induce weight loss by inhibiting the TGF-β/Smad3 signaling pathway and excessive autophagy of adipose tissue， thereby improving the metabolic state of obese rats and reducing the volume of adipocytes.关键词：Acupuncture;Obesity;TGF-β/Smad3 signaling pathway;High-fat diet;Autophagy26|53|0更新时间：2025-12-22
- “最新研究发现，艾灸“天枢”能减轻克罗恩病小鼠肠道炎症，可能通过调节p53/SLC7A11/GSH/GPX4抗氧化轴，减少铁死亡，维持肠道铁稳态。”摘要：ObjectiveTo observe the effects of moxibustion at the acupoint “Tianshu” （ST25） on intestinal inflammatory injury in Crohn’s disease （CD） mice， and to investigate the mechanism by which the tumor protein p53 modulates the antioxidant axis of the solute carrier family 7 member 11 （SLC7A11）/glutathione （GSH）/glutathione peroxidase 4 （GPX4） and ferroptosis in CD mice.MethodsSPF-grade C57BL/6 wild-type mice were randomly divided into normal， model， and moxibustion groups， with 10 mice in each group. In the model and moxibustion groups， the 2% dextran sulfate sodium solution was used to induce CD mice model. The moxibustion group received indirect moxibustion with fine moxa sticks at bilateral ST25 for 30 min per session， once daily， for 14 consecutive days. The body mass， fecal properties， and fecal occult blood were recorded and the disease activity index （DAI） score was determined before and after treatment. At the end of the moxibustion intervention， colonic pathological damage was observed using HE staining. The serum contents of tumor necrosis factor-α （TNF-α）， interleukin （IL）-1β， and IL-17， and the contents of 4-hydroxynonenal （4-HNE）， malondialdehyde （MDA）， GSH， ferrous iron （Fe2+）， and the activity of superoxide dismutase （SOD） of colon tissue were measured by ELISA. The reactive oxygen species （ROS） level in colon tissue was detected by immunofluorescence. The protein expression levels of p53， SLC7A11， and GPX4 in colon tissue were determined by Western blot.ResultsIn the model group， the colon tissue exhibited extensive breaks and defects in the intestinal epithelium， disorganized glandular arrangement， and a large number of inflammatory cell infiltration. After the intervention in the moxibustion group， the intestinal epithelial continuity was restored， the intestinal mucosa was mildly swollen， the glands were more neatly arranged， and a small number of inflammatory cells were occasionally scattered of colon tissue. Compared with the normal group， mice in the model group had obvious disease symptoms， with elevated DAI score （P<0.01）， increased serum contents of TNF-α， IL-1β， and IL-17 （P<0.01）， higher contents of 4-HNE， MDA， and Fe2+ （P<0.01） and reduced GSH contents and SOD activity （P<0.01） in colon tissue， increased expression level of ROS （P<0.01）， and the protein expression of p53 was up-regulated （P<0.01）， the protein expressions of SLC7A11 and GPX4 were down-regulated （P<0.01）. Compared with the model group， mice in the moxibustion group showed alleviated disease symptoms with lower DAI score （P<0.01）， decreased serum contents of TNF-α， IL-1β， and IL-17 （P<0.01）， reduced contents of 4-HNE， MDA， and Fe2+ （P<0.01， P<0.05） and increased GSH contents and SOD activity （P<0.01） in colon tissue， decreased expression level of ROS （P<0.01）， and the protein expression of p53 was down-regulated （P<0.01）， the protein expressions of SLC7A11 and GPX4 were up-regulated （P<0.05， P<0.01）.ConclusionMoxibustion at ST25 reduced intestinal inflammation in CD mice， probably through down-regulating p53 protein expression， enhancing the SLC7A11/GSH/GPX4 antioxidant axis activity， and reducing lipid peroxidation and ferroptosis in colonic tissues， thus maintaining intestinal iron homeostasis.关键词：Crohn’s disease;Moxibustion;Ferroptosis;Tumor protein p53;Tianshu （ST25）;Solute carrier family 7 member 1131|18|0更新时间：2025-12-22
- “最新研究发现，电针“公孙”穴位能调节早发性卵巢功能不全大鼠的性激素水平，减缓卵巢颗粒细胞衰老，提高生育力，可能与调控P53/P21通路相关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Gongsun” （SP4） on tumor suppressor protein 53 （P53）/cyclin-dependent inhibitor 21 （P21） pathway in rats with premature ovarian insufficiency （POI）， so as to investigate its underlying mechanism in alleviating ovarian granulosa cell senescence.MethodsForty female SD rats were randomly divided into control， model， EA and medication groups， with 10 rats in each group. The POI model was established by intraperitoneal injection of cyclophosphamide （50 mg·kg-1·d-1 on the 1st day and 8 mg·kg-1·d-1 from the 2nd to 15th day， for a total of 15 days）. After successful modeling， EA （0.1—1 mA， 2 Hz） was applied at bilateral SP4 for 20 min， once a day for 14 d. Rats of the medication group were treated by gavage of solution of estradiol valerate tablets （0.01 mg/mL， 1 mL/0.1 kg） once a day for 14 d. The changes in the estrous cycle， pregnancy rate and embryo number were observed. The senescence status of ovarian granulosa cells was observed by β-galactosidase staining. The contents of follicle-stimulating hormone （FSH）， estradiol （E2）， luteinizing hormone （LH）， anti-Müllerian hormone （AMH）， transforming growth factor β1 （TGF-β1）， interleukin （IL）-6， and IL-1β in serum were detected by ELISA. The protein and mRNA expression levels of P53， P21， cyclin-dependent kinase inhibitor 2A （P16）， and phosphorylated H2AX （γH2AX） in ovarian tissues were detected by Western blot and qPCR， respectively.ResultsAfter modeling， the rats’ estrus cycles were disordered， mostly stagnated in proestrus and diestrus. Compared with the control group，the pregnancy rate， the number of embryos， and the contents of E2 and AMH were decreased （P<0.01） in the model group， while the contents of serum FSH， LH， IL-6， IL-1β and TGF-β1， the expression of P16， P21， P53 and γH2AX protein and mRNA in ovarian tissue were increased （P<0.01）， with wild SAβ-gal blue staining in ovary. Compared with the model group， the pregnancy rate， the number of embryos， the contents of E2 and AMH were increased （P<0.01） in both EA and medication groups， while the contents of FSH， LH， IL-6， IL-1β and TGF-β1， the protein and mRNA expressions of P53， P21， P16 and γH2AX were significantly decreased （P<0.01， P<0.05）， with reduced SAβ-gal blue staining in ovary.ConclusionsEA of SP4 can regulate the level of serum sex hormones， slow down the aging process of ovarian granulosa cells， improve the ovarian reserve function and fertility of POI rats， which may be related to its function in regulating P53/P21 signaling pathway.关键词：Premature ovarian insufficiency;Electroacupuncture;Cellular Senescence;Gongsun point （SP4）;Ovarian granulosa cells27|22|0更新时间：2025-12-22
- “报道：研究发现不同强度电针治疗周围性面瘫疗效显著，运动阈值电针对脑干神经反射通路有调节作用。”摘要：ObjectiveTo observe the clinical effect of electroacupuncture （EA） at different intensities on peripheral facial paralysis （PFP） and its influence on brainstem excitability.MethodsSixty-six patients with PFP were randomly assigned to a motion-threshold EA group （22 cases， 2 dropped out）， a sensory-threshold EA group （22 cases， 1 dropped out） and a sham-EA group （22 cases， 2 dropped out）. Sibai（ST2）， Dicang（ST4）， Hegu（LI4） and Yuji（LU10） on the affected side were selected in 3 groups and stimulated with EA. In the motion-threshold EA group， the stimulation intensity was determined by the visible muscle jumping in the local area. In the sensory-threshold EA group， the minimal sensory stimulation was adjusted. In the sham-EA group， the skin was not invaded and no electric stimulation was delivered. In each group， the intervention was given once every 3 days， 30 min each time and the duration of treatment was composed of 20 interventions. At the baseline （T0）， after the 10th intervention （T1）， after the 20th intervention （T2） and in 1 month followup after treatment completion （T3）， the House-Brackmann scale for grading facial nerve function （H-B score） and the facial disability index （FDI， comprising physical function ［FDIP］ and social function ［FDIS］） were assessed separately. Besides， the changes of facial nerve injury rate and blink reflex （BR） were observed before and after treatment.ResultsIn the self-group comparison， H-B score at T2 and T3 was improved significantly compared with that at T0 （P<0.001， P<0.05） in the motion-threshold EA and sensory-threshold EA groups separately； FDIP score was increased significantly at T2 and T3 in the motion-threshold EA group （P<0.001）， and FDIP score was increased significantly at T2 in the sensory-threshold EA group （P<0.01）； the FDIS score was reduced significantly from T1 to T3 in the motion-threshold EA group （P<0.05， P<0.001）， and it was reduced significantly at T2 and T3 in the sensory-threshold EA group （P<0.001， P<0.05）. In comparison with the sham-EA group at the same time point， H-B score and FDIS score at T2 and T3 were decreased in the motion-threshold EA group and the sensory-threshold EA group （P<0.01， P<0.05， P<0.001）. After treatment completion， in the motion-threshold EA group and the sensory-threshold EA group， the injury rate of the temporal and buccal branches of the facial nerve was declined （P<0.01， P<0.001） compared with baseline， and it was lower than that of the sham-EA group （P<0.001，P<0.01）. Besides， the R2 latency was shorter in comparison with the baseline in the motion-threshold EA group and the sensory-threshold EA group （P<0.01）， and the latency in the motion-threshold EA group was superior to the sensory-threshold EA group （P<0.05）.ConclusionEA at both motion threshold and sensory threshold obtains a similar therapeutic effect on PFP， and it effectively relieves symptoms of PFP and presents a certain long-term effect. Motor-threshold EA demonstrates modulatory effect on the brainstem’s neural reflex pathway.关键词：Peripheral facial paralysis;Sequelae;Electroacupuncture;Stimulation intensity;Randomized controlled trial;Brainstem excitability27|13|0更新时间：2025-12-22
- “最新研究发现，针刺配合外侧入路坐骨神经阻滞用于全膝置换术后，能降低镇痛药使用，缓解疼痛，改善睡眠，提高恢复质量，安全性良好。”摘要：ObjectiveTo explore the effect of acupuncture intervention combined with lateral approach sciatic nerve block （LSNB） on postoperative analgesia in patients after total knee arthroplasty （TKA）.MethodsA total of 90 patients scheduled for TKA between May 2022 and May 2024 were selected as the study subjects. They were randomly divided into a control group and an observation group using the sealed envelope method， with 45 patients（one case dropped out in each group）in each group. The control group received LSNB for postoperative analgesia， while the observation group received acupuncture intervention （manual acupuncture stimulation of Taichong ［LR3］， Kunlun ［BL60］， Taixi ［KI3］， Shenmai ［BL62］ and Sanyinjiao ［SP6］ on the affected side， and Houxi ［SI3］， Chize ［LU5］， Quchi ［LI11］ and Shousanli ［LI10］ on the healthy side， 30 min per session， once daily for 7 days） in addition to LSNB. Postoperative rescue analgesic usage and sleep monitoring results were recorded. Resting and passive functional exercise-induced limb pain intensity was assessed using the visual analog scale （VAS） at 2 hours （T0）， 6 hours （T1）， 24 hours （T2）， 48 hours （T3）， 72 hours （T4）， and 7 days （T5） postoperatively. Recovery quality was evaluated using the 15-item quality of recovery scale （QoR-15） at T0， T5， 1 month （T6）， and 3 months （T7） postoperatively. The safety of the analgesic regimens was also assessed.ResultsThe frequency of rescue analgesic use and the total dosage consumed were significantly lower in the observation group than in the control group （P<0.05）. There were no statistically significant differences between two groups in the sleep latency and N1 sleep stage proportion within 0—12 hours after operation. However， within 12—72 hours postoperatively， the observation group had fewer awakenings， a higher proportion of N3 deep sleep， and higher sleep efficiency than the control group （P<0.05）. At T5， VAS scores at rest and during passive functional exercise were significantly lower in the observation group than in the control group （P<0.05）. The incidence of adverse reactions was below 10% in both groups， with no statistically significant difference between the two groups . QoR-15 scores were significantly higher in the observation group than in the control group at T5 and T6 （P<0.05）.ConclusionCompared with LSNB alone， acupuncture intervention combined with LSNB after TKA can reduce the need for rescue analgesia， relieve early postoperative pain， and improve the quality of sleep and postoperative recovery， and is safe.关键词：Total knee arthroplasty;Postoperative analgesia;Acupuncture intervention;Lateral approach sciatic nerve block;Sleep quality26|19|0更新时间：2025-12-22
- “在针刺临床试验领域，专家建立了过程评价框架，破解复杂系统特性难题，为循证医学提供方法学支持，增强针灸国际化趋势。”摘要：The root cause of the lagging evidence level in clinical trials of acupuncture lies in the complex system characteristics of acupuncture intervention. Process evaluation can solve the above difficulties through systematically monitoring the implemention of fidelity， verifying causal mechanism， analyzing situational factors and other approaches. In view of the complexity of operation， this study proposes a framework for process evaluation， i.e.， constructing “fidelity index” can enable quantitative measurement of differences in technical schools， and can develop “total stimulus dose” to standardize intervention dosage； using “capability matrix” can model the dynamic interaction between clinicians and patients， so as to cope with the uncertainty， and applying “therapeutic alliance scale” to capture the variation of human factors； for background effect visualization， a “resource heatmap” can be created to integrate macro-variables such as medical insurance policy coverage and cultural acceptance； and using the “latent category growth model” can identify heterogeneous trajectories and establish a closed loop of monitoring， optimization and feedback in terms of dynamic tracking of efficacy. By the fidelity index， capability matrix， resource heatmap， and dynamic trajectory model， this framework can transform the abstract complexity into the intervention target， which can significantly improve the validity and extensibility of the experiment. Process evaluation can drive the leap from empirical inheritance to evidence-based medicine in acupuncture research， which will not only provide methodological support for the modernization of traditional medicine， but also can enhance the internationalization trend of acupuncture by optimizing localization adaptation strategies and policy evidence transformation paths.关键词：Acupuncture;Process evaluation;Complex interventions;Clinical trials;Fidelity34|25|0更新时间：2025-12-22
- “最新研究总结了针灸辅助体外受精的临床证据，并对比了医生和患者的关注点差异，为未来研究指明方向。”摘要：ObjectiveTo summarize and evaluate the existing evidence on acupuncture-assisted in vitro fertilization-embryo transfer （IVF-ET）， to survey clinicians and patients regarding gaps between the evidence and clinical concerns， and to identify directions for future clinical research.MethodsRandomized controlled trials （RCTs） of acupuncture-assisted IVF-ET were retrieved from CNKI， SinoMed， VIP， WanFang， PubMed， Embase， and Cochrane Library from inception through December 31， 2024. Two researchers independently screened the literature and extracted data. The methodological quality of the included studies was assessed using the Risk of Bias （ROB） 1.0 tool. Questionnaires， developed based on prior research and expert discussions， were distributed to clinicians and patients. Discrepancies between the evidence and clinical priorities were analyzed across the population，intervention，comparison and outcome dimensions.ResultsA total of 143 RCTs with potential risks of bias were included. Questionnaires were completed by 103 clinicians and 108 patients. Comparisons reveal that elderly patients （>35 years old）， patients with long-standing infertility （>5 years）， and patients undergoing frozen embryo transfer are more focused on by clinicians， but the evidence supporting these concerns is limited. In acupuncture modalities， filiform needles have strong evidence and high recognition； moxibustion and ear acupuncture attract patients’ attention but have limited evidence， while transcutaneous electrical acupoint stimulation shows the opposite pattern. Regarding acupuncture timing and frequency， doctors focus on the pre-oocyte retrieval， the retrieval-to-transfer， and post-embryo transfer periods， with low evidence in some periods. High-evidence scenarios （≥3 pre-oocyte retrieval， 1 post-embryo transfer） receive moderate-to-low doctors’ attention. For control measures， comparisons with Western and Chinese medicine attract doctors’ attention but with limited evidence； blank controls show the opposite trend. In terms of outcomes， live birth rate attracts attention but has limited evidence. Mental health outcomes have little evidence and show substantial disparities between doctor and patient priorities. Complications have weak evidence yet high doctors’ focus. Clinical pregnancy rate aligns more closely with doctor-patient attention.ConclusionFuture studies should enhance the quality of RCTs， broaden the range of included samples， and perform stratified analyses. High-quality trials are needed to evaluate the efficacy and safety of different interventions， determine the optimal acupuncture timing and frequency， and design clinically relevant control groups. Outcome measures should be expanded， and long-term follow-up should be emphasized to advance acupuncture’s value in assisted reproduction.关键词：Acupuncture;In vitro fertilization-embryo transfer;Evidence discrepancy;Clinical decision-making25|17|0更新时间：2025-12-22
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Clinical efficacy of intranasal acupuncture in the treatment of allergic rhinitis： a Meta-analysis AI导读
“最新研究显示，鼻内针治疗变应性鼻炎效果优于西药和鼻外针刺，且安全性良好。”摘要：ObjectiveTo evaluate the efficacy and safety of intranasal acupuncture for allergic rhinitis （AR）.MethodsRandomized controlled trials on intranasal acupuncture for AR were searched in China National Knowledge Infrastructure， Wanfang Data Knowledge Service platform， VIP Database， PubMed， Web of Science， Embase， and The Cochrane Library from their inception to October 2024. The basic information， evaluation of the therapeutic efficacy， related scales， and laboratory indexes were screened and extracted by 2 independent reviewers. The scales included the Total Nasal Symptom Score （TNSS）， the Total Nasal Symptom and Sneezing Score （TNNSS）， Rhinoconjunctivitis Quality of Life Questionnaire （RQLQ）. Laboratory indicators focused on serum total IgE and serum interleukin-4 （IL-4） contents. Meta-analysis was conducted using RevMan software and Stata software.ResultsA total of 19 randomized controlled trials involving 1 482 participants were included. The Meta-analysis indicated that， compared with conventional western medicines and nasal-external acupuncture， the intranasal acupuncture group achieved greater reductions in TNSS ［MD=-1.67， 95% CI （-2.30， -1.04）， P<0.000 01］， RQLQ score ［MD=-3.14， 95% CI（-4.16， -2.12）， P<0.000 01］， and TNNSS ［MD=-0.52， 95% CI （-0.67，-0.36）， P<0.000 01］ from baseline to post-treatment， and greater decreases in serum IgE ［SMD=-1.17， 95% CI （-1.46， -0.87）， P=0.000 01］ and IL-4 ［SMD=-1.14， 95% CI （-1.80， -0.49）， P=0.000 6］ levels. Adverse events were mainly mild epistaxis and localized pain.ConclusionIntranasal acupuncture appears superior to nasal-external acupuncture and western medicines in reducing nasal symptom severity and improving life quality in AR， with no obvious adverse events reported. However， because the existing studies generally have small sample sizes， limited methodological rigor in randomized designs， and substantial heterogeneity across studies， the reliability of the findings is limited. Therefore， large-sample， multicenter， randomized controlled clinical trials are needed to improve the quality level of the evidence.关键词：Allergic rhinitis;Intranasal acupuncture;Randomized controlled trial;Clinical efficacy;Meta-analysis38|29|0更新时间：2025-12-22
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