最新刊期
卷 50 ， 期 2 ， 2025
- “最新研究发现，“秩边”透“水道”针刺能改善卵巢低反应小鼠卵巢功能，可能通过调节凋亡相关因子表达发挥作用。”摘要：ObjectiveTo observe the effect of penetrative needling of “Zhibian” （BL54） through “Shuidao” （ST28） on the expressions of internal and external apoptosis-related factors in mice with poor ovarian response （POR）， so as to explore its mechanisms underlying improvement of POR.MethodsSixty-nine female C57BL/6N mice were randomly divided into blank， model and acupuncture groups （n=23 in each group）. The POR model was established by gavage of tripterygium wilfordii polyglucoside suspension （50 mg·kg-1）. After successful modeling， the rats in the acupuncture group received penetrative needling of BL54 through ST28， with the needle retained for 20 min， once a day for 14 days. Thirty healthy male mice of the same strain aged 7—8 weeks were co-housed with female mice in a 1∶1 ratio. Vaginal exfoliative cell smears were used to observe the changes in the estrous cycle of female mice. The number of follicles were observed under light microscope， wet weight of ovaries was recorded and ovarian index was calculated. The pregnancy rate and number of embryos were observed. ELISA method was used to detect the low levels of anti Mullerian hormone （AMH）， follicle stimulating hormone （FSH）， luteinizing hormone （LH）， and estradiol （E2） contents in the serum of female mice. HE staining was used to observe the morphology of ovarian tissue. TUNEL staining was used to observe the apoptosis rate of ovarian cells. The mRNA and protein expression levels of cysteine-aspartic acid protease （Caspase）-3， Bcl-2-associated X protein （Bax）， B-cell lymphoma-2 （Bcl-2）， fas cell surface death receptor （Fas）， and fas-associated death domain （FADD） in ovarian tissue were detected by real-time quantitive PCR and Western blot， respectively.ResultsCompared with the blank group， the rate of estrous cycle disorder， the contents of FSH and LH in serum， the rate of ovarian apoptosis， the mRNA and protein expression levels of Caspase-3， Bax， Fas and FADD in ovarian tissues were significantly increased （P<0.01）； whereas the pregnancy rate， the number of embryos， the number of eggs obtained， ovarian wet weight， ovarian index， the contents of AMH and E2， the mRNA and protein expression levels of Bcl-2 were significantly decreased （P<0.01） in the model group. Following the intervention， the above-mentioned indexes were all reversed in the acupuncture group （P<0.01， P<0.05）.ConclusionPenetrative needling of BL54 through ST28 can increase the ovarian response in POR mice， which may be associated with its function in regulating the expression of internal and external apoptosis-related factors in ovarian tissue.关键词：Poor ovarian response;“Zhibian” （BL54） through “Shuidao” （ST28）;Acupuncture;Apoptosis80|19|0更新时间：2025-06-21
- “最新研究发现，针刀疏筋解结术能减轻类风湿关节炎大鼠炎性反应，缓解关节损伤，抑制骨破坏，可能通过抑制PI3K/AKT/NFATc1通路实现。”摘要：ObjectiveTo observe the effect of acupotomy tendon-sparing and knot-dissolving technique on bone destruction and phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT）/nuclear factor of activated T cells c1 （NFATc1） pathway in rats with rheumatoid arthritis （RA）， so as to investigate the underlying mechanism.MethodsForty SD rats were randomly divided into normal， model， medication （tripterygium wilfordii）， and acupotomy groups， with 10 rats in each group. Except for the normal group， the rest of the rats were injected with bovine type II collagen emulsion at the base of tails to establish a collagen-induced RA model. The acupotomy group was treated with acupotomy tendon-sparing and knot-dissolving technique， once every 3 days， with a continuous intervention for 9 times. The medication group was given tripterygium wilfordii polyglycoside suspension （8 mg·kg-1） by gavage， once a day for 28 days continuously. The swelling degree of the ankle joint and the arthritis index score of the rats were observed. Micro-CT scanning was used to observe the degree of bone destruction in the left ankle joint. HE staining and ferruben-solid green staining were used to observe the pathological morphological changes of synovial and cartilage tissue respectively. ELISA was used to detect the contents of tumor necrosis factor （TNF）-α， interleukin （IL）-1β， IL-6 and IL-17 in synovial tissue. Tartrate-resistant acid phosphatase （TRAP） staining was used to observe the number of osteoclasts in the left ankle joint. Western blot was used to detect the expression levels of NFATc1， p-PI3K and p-AKT proteins in synovial tissue. Immunohistochemistry was used to detect the positive expressions of matrix metalloproteinase-9 （MMP9）， cathepsin K （CTSK） and TNF receptor-associated factor 6 （TRAF6） in the synovial tissue of the ankle joint.ResultsIn comparison with the normal group， the bones of the ankle joint and toes of rats were severely eroded， with an uneven surface in the model group； there was a large number of inflammatory cell infiltrations in the synovial tissue， obvious damage to the articular cartilage， and disordered arrangement of synovial cells； the cartilage matrix was damaged， the cartilage layer was rough， and the subchondral bone structure was disordered. In comparison with the model group， the above histopathological changes in the medication group and the acupotomy group were alleviated. Compared with the normal group， the joint swelling degree， arthritis index， the ratio of bone surface area to bone volume （BS/BV）， trabecular number （Tb.N）， trabecular separation （Tb.Sp）， the contents of TNF-α， IL-1β， IL-6 and IL-17 in synovial tissue， the number of osteoclasts in the joint， the expressions or ratios of NFATc1， p-PI3K/PI3K， p-AKT/AKT proteins in synovial tissue， and the positive expressions of TRAF6， CTSK and MMP9 proteins in synovial tissue in the model group were significantly increased （P<0.01）， while the bone mineral density （BMD）， bone volume fraction （BV/TV） and trabecular thickness （Tb.Th） were significantly decreased （P<0.01）. Compared with the model group， the joint swelling degree， arthritis index， BS/BV， Tb.Sp， the contents of TNF-α， IL-1β， IL-6 and IL-17 in synovial tissue， the number of osteoclasts， the expressions or ratios of NFATc1， p-PI3K/PI3K， p-AKT/AKT proteins in synovial tissue， and the positive expressions of TRAF6， CTSK and MMP9 proteins in synovial tissue in the medication group and the acupotomy group were significantly decreased （P<0.01， P<0.05）， while BMD， BV/TV and Tb.Th were significantly increased （P<0.01， P<0.05）， and Tb.N in the acupotomy group was significantly decreased （P<0.01）.ConclusionAcupotomy tendon-sparing and knot-dissolving technique can effectively reduce the inflammatory response， relieve the pathological damage of joint tissues and inhibit bone destruction in RA rats， and its mechanism may be related to inhibiting the activation of the PI3K/AKT/NFATc1 pathway.关键词：Acupotomy tendon-sparing and knot-dissolving technique;Rheumatoid arthritis;Bone destruction;Phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt）/nuclear factor of activated T cells c1 （NFATc1） pathway;Osteoclast65|9|0更新时间：2025-06-21
- “最新研究发现，电针“夹脊”能减轻脊髓损伤小鼠神经炎性反应及胶质瘢痕形成，改善神经功能恢复，可能与下调中性粒细胞胞外陷阱表达水平有关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on neuroinflammatory response and glial scar formation Via regulating expression of neutrophil extracellular traps （NETs） in mice with spinal cord injury （SCI）.MethodsSixty female C57BL/6 mice were randomized into sham operation， model， EA， DNase1 and EA+DNase1 groups， with 12 mice in each group. The SCI model was established by clamping the spinal cord with a serrefine after laminectomy at the 11th thoracic vertebra （T11）. EA （1.5 Hz/7.5 Hz， 1 mA） was applied to bilateral “Jiaji” （EX-B2， T10， T12） for 10 min， once a day for 14 consecutive days， for mice of the EA and EA+DNasel groups. Mice of the DNase1 and EA+DNase1 groups were intraperitoneally injected with NETs inhibitor （DNase1， 50 μg/mice）， twice daily for 14 days. The motor function was evaluated by BBB score. Histopathological changes of the injured area of the spinal cord were determined by H.E. staining. The positive expressions of citrullinated histone H3 （citH3） and glial fibrillary acidic protein （GFAP） in the spinal cord of mice were observed by immunofluorescence. Western blot was used to detect the expressions of citH3， elastase （NE）， myeloperoxidase （Mpo）， cyclic guanosine monophosphate-adenylate synthase （cGAS）， interferon gene stimulator （STING）， GFAP and Neurocan in spinal cord tissue. The contents of inflammatory factors interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） in spinal cord and serum were detected by ELISA .ResultsAfter SCI， the BBB scores were obviously decreased （P<0.05）， while the protein relative expressions of citH3， NE， Mpo， cGAS， STING， GFAP， and Neurocan in spinal cord tissue， and the fluorescence intensities of citH3 and GFAP， as well as the contents of IL-1β and TNF-α in serum and spinal cord tissue were all increased in the model group relevant to the sham operation group （P<0.05， P<0.01）. In the EA， DNase1 and EA+DNase1 groups， SCI-induced reduction of BBB scores， and increases of the protein relative expressions of citH3， NE， cGAS， STING， GFAP， and Neurocan， and the fluorescence intensities of citH3 and GFAP， as well as the contents of IL-1β and TNF-α were reversed relevant to the model group （P<0.05， P<0.01）. The expression level of Mpo protein decreased （P<0.05， P<0.01） in spinal cord of rats in the EA and EA+DNasel groups relevant to the model group. The therapeutic effects of EA+DNase1 were better than EA or DNase1 （P<0.05， P<0.01）. H.E. staining showed neuronal degeneration， nuclear pyknosis and inflammatory cell infiltration in the injured spinal cord tissue in the model group， which was relatively milder in the three intervention groups.ConclusionEA of EX-B2 can alleviate neuroinflammatory reactions and glial scar formation in SCI mice， and improve the recovery of neurological function after SCI， which maybe related to its function in down-regulating NETs expression.关键词：Spinal cord injury;Electroacupuncture;Neutrophil extracellular traps;Neuroinflammatory response;Glial scar97|13|0更新时间：2025-06-21
- “最新研究发现，电针通过激活SIRT1/FOXO1信号通路，促进胆固醇流出，减轻非酒精性脂肪肝大鼠的肝脏损伤。”摘要：ObjectiveTo investigate the effects of electroacupuncture （EA） on liver function， lipid metabolism， hepatic histopathology， and the expression of molecules in the SIRT1/FOXO1 signaling pathway in rats with non-alcoholic fatty liver disease （NAFLD）， as well as to explore its potential underlying mechanisms.MethodsA total of 13 SD rats were assigned to the blank group and fed a standard diet. An NAFLD model was established in 43 SD rats through a 12-week high-fat diet. Three rats from each group were randomly selected to confirm successful model establishment. After confirmation， rats in the modeling group were randomly divided into four groups： model group， EA group， EA+inhibitor group， and agonist group， with 10 rats in each group. The blank and model groups underwent immobilization three times per week for four weeks. The agonist group received intraperitoneal injections of the SIRT1 agonist resveratrol （200 mg/kg） three times per week for four weeks. The EA group received EA at “Fenglong” （ST40） and “Zusanli” （ST36） acupoints for 30 minutes， three times per week for four weeks. The EA+inhibitor group was administered the SIRT1 inhibitor EX527 （5 mg/kg） intraperitoneally， with the remaining treatment identical to that of the EA group. After the interventions， contents of serum high-density lipoprotein cholesterol （HDL-C）， low-density lipoprotein cholesterol （LDL-C）， total cholesterol （TC）， triglycerides （TG）， as well as activities of alanine aminotransferase （ALT） and aspartate aminotransferase （AST） were measured by using colorimetric method. Liver histopathology was assessed using hematoxylin-eosin （HE） and Oil Red O staining. The protein and mRNA expressions of SIRT1， FOXO1， and ABCA1 in liver tissue， as well as the protein expression of acetylated FOXO1 （AC-FOXO1）， were detected using Western blot and PCR.ResultsCompared with the blank group， the model group exhibited significantly decreased serum HDL-C contents （P<0.05）， along with increased contents of LDL-C， TC， TG， and activities of ALT and AST （P<0.01， P<0.05）； histological analysis revealed disorganization of hepatocytes and pronounced fat vacuolization， additionally， the expressions of hepatic SIRT1， FOXO1， and ABCA1 proteins and mRNA were reduced （P<0.05， P<0.01）， whereas AC-FOXO1 protein expression was elevated （P<0.05）. Compared with the model group， the EA and agonist groups demonstrated increased serum HDL-C contents （P<0.05）， along with decreased contents of LDL-C， TC， TG， and ALT and AST activities （P<0.01， P<0.05）； histological results showed improved hepatocyte morphology and reduced steatosis， along with elevated expression of SIRT1， FOXO1， and ABCA1 proteins and mRNA （P<0.05， P<0.01） and decreased AC-FOXO1 protein expression （P<0.05）. Compared with the EA group， the EA+inhibitor group had significantly lower serum HDL-C contents （P<0.05）， and higher contents of LDL-C， TC， TG， and activities of ALT and AST （P<0.01， P<0.05）； histological analysis revealed more fat vacuoles and pronounced lipid droplet deposition， alongside decreased hepatic SIRT1， FOXO1， and ABCA1 protein and mRNA expressions （P<0.05， P<0.01）， and elevated AC-FOXO1 protein expression （P<0.05）.ConclusionEA may alleviate liver injury in NAFLD rats by activating the SIRT1/FOXO1 signaling pathway to promote cholesterol efflux.关键词：Non-alcoholic fatty liver disease;Electroacupuncture;Silent information regulator 1;Forkhead box protein O169|15|0更新时间：2025-06-21
- “最新研究发现，针刺能通过抑制cGAS-STING信号通路，减轻帕金森病大鼠海马神经元损伤，改善认知障碍。”摘要：ObjectiveTo observe the effect of acupuncture on neuro-inflammation in rats with cognitive impairment induced by Parkinson’s disease （PD） based on the cyclic GMP-AMP synthase （cGAS）-stimulator of interferon genes （STING） signaling pathway.MethodsSD rats were randomly grouped into sham operation， model， acupuncture， acupuncture+empty and acupuncture+cGAS over-expression groups， with 12 rats in each group. The PD model was established by injecting 6-hydroxydopamine （6-OHDA， 8 μg/rat） into the substantia nigra pars compacta of the left midbrain （AP：-5.2 mm， ML：2.1 mm， DV：-7.8 mm）. Acupuncture was conducted for rats in the acupuncture， acupuncture+empty and acupuncture+cGAS over-expression groups， for 20 min， once daily； empty plasmid or cGAS over-expression plasmid was injected into the substantia nigra pars compacta of the left midbrain for rats of the acupuncture+empty or acupuncture+cGAS over-expression groups respectively， twice a week. The same volume of 0.9% sodium chloride solution was injected for rats in the sham operation， model and acupuncture groups， twice a week. All above interventions were performed for 2 weeks. The rotation behavior of rats was detected by apomorphine-induced rotation experiment. The cognitive function was detected by Morris water maze test and dark avoidance test. Nissl staining was used to detect the pathological morphology and the number of hippocampal neurons. TUNEL staining was used to detect the apoptosis rate of hippocampal neurons. The contents of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in serum and midbrain tissue were detected by ELISA. Western blot was used to detect the expression levels of cGAS and STING proteins in midbrain tissue.ResultsCompared with the sham operation group， the rotation speed， the escape latency in the Morris water maze test and the number of errors in the dark avoidance test， the apoptosis rate of hippocampal neurons， the contents of TNF-α and IL-1β in serum and midbrain tissue， and the expression levels of cGAS and STING protein in midbrain tissue were increased （P<0.05）， while the times of crossing the original platform in the Morris water maze test， the latency in the dark avoidance test and the number of hippocampal neurons were decreased （P<0.05） in the model group， the hippocampal neurons were disordered. All the indexes mentioned above were reserved in the acupuncture and acupuncture+empty groups （P<0.05）， and there was no significant difference of all indexes between these two groups. After injection of cGAS over-expression plasmid， compared with acupuncture group， the rotation speed， the escape latency in the Morris water maze test and the number of errors in the dark avoidance test， the apoptosis rate of hippocampal neurons， the contents of TNF-α and IL-1β in serum and midbrain tissue， and the expression levels of cGAS and STING protein in midbrain tissue were increased （P<0.05）， while the times of crossing the original platform in the Morris water maze test， the latency in the dark avoidance test and the number of hippocampal neurons were decreased （P<0.05）， the hippocampal neurons were disordered.ConclusionAcupuncture can improve the cognitive impairment of PD rats， which may be related to its functions in blocking the cGAS-STING signal transduction， inhibiting the neuroinflammatory response， and reducing the damage and apoptosis of hippocampal neurons.关键词：cGAS-STING signaling passway;Acupuncture;Parkinson’s disease;Cognitive impairment;Neuro-inflammation77|16|0更新时间：2025-06-21
- “最新研究发现，电针特定穴位能改善帕金森病小鼠运动障碍，减轻细胞凋亡，可能通过调节BFGF/GSK-3β通路发挥作用。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on basic fibroblast growth factor （BFGF）/glycogen synthase kinase-3β （GSK-3β） pathway in Parkinson’s disease （PD） mice， so as to explore the potential mechanisms of EA in treating PD.MethodsFifty C57/BL6 male mice were randomly divided into the control， model， EA， inhibitor and EA+inhibitor （combination） groups， with 10 mice in each group. The PD mouse model was established by intraperitoneal injection of 1-methyl-4-phenyl-1，2，3，6-tetrahydropyridine. Mice in the EA group received EA stimulation at “Fengfu” （GV16）， “Taichong”（LR3） and “Zusanli”（ST36） for 30 min， once daily for 12 days. The inhibitor group received gavage of fibroblast growth factor receptor （FGFR） inhibitor AZD4547 （12.5 mg·kg-1·d-1） for 12 consecutive days. The open box experiment was used to observe the autonomous movement ability of mice. The expression level of tyrosine hydroxylase （TH） in substantia nigra was detected by immunohistochemistry. The apoptosis rate of substantia nigra cells was measured by TUNEL method. The relative protein expression levels of BFGF， phosphorylated phosphatidylinositol 3-kinase （p-PI3K）， phosphorylated protein kinase B （p-AKT）， p-GSK-3β， B-lymphomatoma-2 gene （Bcl-2）， and Bcl associated X protein （Bax） in substantia nigra were detected by Western blot.ResultsAfter modeling， compared with the control group， the total movement distance was shorten and the duration of rest was prolonged in the open box experiment （P<0.01）， the average absorbance of TH in the substantia nigra of the midbrain was decreased （P<0.01）， and the apoptosis rate of substantia nigra cells in the midbrain was increased （P<0.01）， the protein expression levels of BFGF， p-PI3K， p-AKT， p-GSK-3β and Bcl-2 were decreased （P<0.01）， while the expression level of Bax protein was increased （P<0.01） in the model group. Compared with the model and the combination groups， the total movement distance was prolonged and the duration of rest was shorten （P<0.01）， the average absorbance of TH was increased （P<0.05）， and the apoptosis rate was decreased （P<0.01，P<0.05）， the expression levels of BFGF， p-PI3K， p-AKT， p-GSK-3β and Bcl-2 were increased （P<0.01，P<0.05）， while the expression level of Bax was decreased （P<0.05） in the EA group.ConclusionEA at GV16， LR3 and ST36 can alleviate motor disorders， reduce cell apoptosis， protect dopaminergic neurons in mice with PD， which may be related to its effect in regulating the BFGF/GSK-3β pathway.关键词：Parkinson’s disease;Electroacupuncture;Basic fibroblast growth factor;Glycogen synthase kinase-3β58|14|0更新时间：2025-06-21
- “最新研究发现，针刺“大椎-身柱”穴位能改善过敏性哮喘大鼠气道重塑，可能通过下调DEK表达，抑制PI3K/AKT激活，延缓上皮间质转化进程。”摘要：ObjectiveTo observe the effect of acupuncture at “Dazhui” （GV14） and “Shenzhu” （GV12） on DEK/phosphorylated phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） pathway and epithelial-mesenchymal transition related factors in rats with allergic asthma （AA）， so as to explore its potential mechanism in the treatment of AA.MethodsSixty SD rats were randomly divided into normal， model， acupuncture， dexamethasone （DEX） and sham-acupuncture groups， with 12 rats in each group. The AA model was made by intraperitoneal injection of mixture solution of ovalbumin and aluminium hydroxide gel （on day 1 and 7） and followed by inhalation of atomized 1% ovalbumin （30 min， from day 15， once daily for 14 days）. Rats in the acupuncture group received acupuncture at GV14 and GV12， while rats in the sham-acupuncture group received acupuncture at the non-meridian and non-acupoint point， both for 20 min， once daily for 14 days. Rats of the DEX group received intraperitoneal injection of DEX （0.5 mg/kg）， once daily for 14 days. H.E. staining was used to observe the changes of pathological morphology in lung tissue. ELISA was used to detect the levels of interleukin （IL）-4 and IL-5 in broncho alveolar lavage fluid （BALF）， and serum immunoglobulin IgE and IgM. Western blot was used to detect the expressions of DEK， PI3K， AKT， phosphorylated （p）- PI3K， p-AKT， and E-cadherin in lung tissue. Immunofluorescence staining was used to detect the positive expressions of DEK， p-PI3K， p-AKT， and E-cadherin in lung tissue.ResultsCompared with the normal group， the levels of IL-4， IL-5 in BALF and serum IgE and IgM， the protein expression levels of DEK， p-PI3K and p-AKT in lung tissue， the positive expression levels of DEK， p-PI3K and p-AKT were increased in the model group （P<0.01）， while the protein expression and positive expression levels of E-cadherin were decreased （P<0.01）. In comparison with the model group， the contents of IL-4， IL-5 in BALF and serum IgE and IgM， the protein expressions of DEK， p-PI3K and p-AKT， the positive expression levels of DEK and p-PI3K in the acupuncture and DEX groups were decreased （P<0.01， P<0.05）， while the positive expression of E-cadherin was increased （P<0.05）. The positive expression level of p-AKT in the acupuncture group was decreased （P<0.05）. The level of IL-5 in BALF was decreased （P<0.01） in the sham-acupuncture grourp relative to the model group. H.E. staining showed that the rats in the model and sham-acupuncture groups exhibited thickened bronchial walls and increased infiltration of inflammatory cells， while these situations were obviously milder in both acupuncture and DEX groups.ConclusionAcupuncture can improve the airway remodeling of AA rats induced by ovalbumin， which may be associated with its functions in down-regulating the expression of DEK， inhibiting the activation of PI3K/AKT， and delaying the process of epithelial-mesenchymal transition.关键词：Acupuncture;Allergic asthma;Epithelial-mesenchymal transition;DEK;Phosphorylated phosphatidylinositol 3-kinase （p-PI3K）;Protein kinase B （AKT）70|18|0更新时间：2025-06-21
- “最新研究发现，补肾通督外治法能有效改善肾阳亏虚型强直性脊柱炎患者症状，降低疾病活动度，减轻炎性反应，疗效显著且安全。”摘要：ObjectiveTo observe and evaluate the effectiveness and safety of Bushen Tongdu （reinforcing the kidney-yang and regulating the Governor Vessel） external treatment for patients with kidney-yang deficiency type ankylosing spondylitis （AS）.MethodsPatients with Kidney-yang deficiency type AS were randomly divided into an observation group（36 cases，4 cases dropped out） and a control group（36 cases，3 cases dropped out）. The observation group were received the Bushen Tongdu external treatment which was a combination of Ren Du Zhou-Tian moxibustion and acupoint embedding therapy， i.e. performing ginger-juice soaked nonwoven separated moxibustion first at the back covering the region from Dazhui （GV14） to Yaoshu （GV2） for 1 h， and then at the thoracoabdominal region covering the part from Tiantu （CV22） to Zhongji （CV3） for 1 h， followed by performing acupoint catgut embedding at one or two pairs of GV14， Shenshu （BL23）， Dachangshu （BL25）， Xuehai （SP10）， and Zusanli （ST36）， once a week， for 8 weeks. The control group received oral administration of salazosulfapyridine capsules （0.2 g/time）， once a day for 8 weeks. Changes of the patients’ conditions were evaluated by using traditional Chinese medicine （TCM） syndrome score （0 to 3 points for cold and painful lower back， morning stiffness， limited lumbar movement， soreness and weakness of waist and knees， etc.） and the total score （0 to 18 points）， the Bath ankylosing spondylitis disease activity index （BASDAI） score （0 to 10 points for fatigue， physical discomfort caused by pain， swelling， or tenderness in the spine and peripheral joints， duration and severity of morning stiffness， etc）. The therapeutic effect was assessed . The safety observation included fainting during acupuncture or moxibustion treatment， burn， etc. The contents of serum interleukin-6 （IL-6）， tumor necrosis factor-alpha （TNF-α）， and matrix metalloproteinase-3 （MMP-3） were detected before and after the treatment.ResultsAfter 8 weeks of treatment， the TCM syndrome score， total score， BASDA score， contents of serum IL-6， TNF-α and MMP-3 were strikingly decreased in both groups compared with those of their own pre-treatment （P<0.05）， and the therapeutic effect of the observation group was significantly superior to that of the control group in lowering the scores of cold and painful lower back， weakness in the lower back and knees， preference for warmth and aversion to cold， pain worsened by cold， and total score， BASDA score， and contents of serum IL-6， TNF-α and MMP-3 （P<0.05）. The total effective rate of observation group was 90.63% （29/32）， which was higher （P<0.05） than that of control group （75.76%， 25/33）.ConclusionThe Bushen Tongdu external treatment method significantly improves AS patients’ TCM clinical symptoms， alleviates inflammatory reactions， and demonstrates a significant efficacy with good safety.关键词：Bushen Tongdu external treatment method;Ankylosing spondylitis;Ren Du Zhou-Tian moxibustion;Acupoint embedding therapy;Kidney-yang deficiency108|21|0更新时间：2025-06-21
- “最新研究发现，经皮耳迷走神经刺激联合自控静脉镇痛可有效减轻全膝关节置换术后疼痛，降低恶心发生率和阿片类药物用量。”摘要：ObjectiveTo explore the effect of transcutaneous auricular vagus nerve stimulation （taVNS） combined with patient-controlled intravenous analgesia （PCIA） on postoperative pain in patients undergoing total knee arthroplasty （TKA）.MethodsA total of 140 patients with TKA were randomly divided into the taVNS group （n=70， with 5 dropped-out） and the sham stimulation group （n=70， with 6 dropped-out）. The taVNS group received taVNS at the left ear conchae combined with PCIA treatment， while the sham stimulation group received sham taVNS at the left ear conchae combined with PCIA treatment. The time-weighted average （TWA） pain score， visual analogue scale （VAS） score of 1—7 days after surgery， the occurrence of adverse reactions such as nausea， vomiting， skin pruritus， drowsiness， respiratory depression， and the number of PCIA pump compressions within 48 hours after surgery were observed and compared between the 2 groups. The Beck Anxiety Inventory （BAI） scores， Bake Depression Inventory （BDI） scores， and Pittsburgh Sleep Quality Index （PSQI） scores were obtained on the day before surgery and 7 days after surgery.ResultsCompared with the sham stimulation group， the TWA pain score of the taVNS group was significantly decreased at the 7th day postoperatively （P<0.01）， and the VAS scores of the taVNS group were significantly lower than those of the sham stimulation group on postoperative days 3rd， 4th， and 5th （P<0.001，P<0.01，P<0.05）， with no statistical significance in VAS scores on postoperative days 1st， 2nd， 6th， and 7th. Compared with the sham stimulation group， the incidence of nausea and the number of PCIA pump compressions within 48 hours after surgery in the taVNS group was significantly decreased （P<0.05）. There was no statistical significance in the differences between the BDI， BAI， and PSQI scores before and after surgery in both groups. PSQI scores in the sham stimulation group and taVNS group were significantly lower than those in the same group 1 day before surgery （P<0.01， P<0.001）.ConclusionThe taVNS combined with PCIA can reduce postoperative pain， the incidence of nausea， and the opioid dosage in TKA patients.关键词：Transcutuneous auricular vagus nerve stimulation;Postoperative analgesia;Patient-controlled intravenous analgesia;Total knee arthroplasty161|30|0更新时间：2025-06-21
- “最新研究发现，揿针疗法结合推拿手法及主动功能锻炼能有效改善颈型颈椎病患者的颈部功能障碍和疼痛症状，提高临床疗效，且安全性良好。”摘要：ObjectiveTo explore the clinical efficacy of acupressure therapy combined with Tuina and active functional exercise in the treatment of neck-type cervical spondylosis.MethodsSixty patients with cervical spondylosis were randomly divided into a control group and an experimental group， with 30 cases in each group. The control group was treated with Tuina and active functional exercise. Tuina therapy lasted 10–15 min per session， twice a week for 4 weeks. Functional exercises were performed once in the morning and once in the evening， each session lasting about 10 min， for a total of 4 weeks. The experimental group received the same treatment as the control group combined with ringheaded thumb-tack needle therapy which was applied to 5 Ashi points， with needle retention for 3 days followed by 1 day of rest， for a total of 4 weeks. The neck disability index （NDI） and the short-form of McGill pain questionnaire （SF-MPQ） including visual analog scale （VAS）， present pain intensity （PPI） and pain rating index （PRI） were used before treatment， after 2 weeks and after 4 weeks of treatment. The modified Macnab efficacy evaluation criteria were used to assess treatment outcomes， and safety was also evaluated.ResultsCompared with baseline， both groups showed significant reductions in NDI， VAS， PPI， and PRI scores after 2 and 4 weeks of treatment （P<0.01）. The improvement in the experimental group was significantly greater than that in the control group （P<0.05）. The clinical efficacy rate （excellent and good） was 60.0% （18/30） in the experimental group， compared to 30.0% （9/30） in the control group， with the experimental group showing better clinical outcomes （P< 0.05）. No adverse reactions were reported during the study.ConclusionAcupressure therapy combined with Tuina and active functional exercise can effectively improve symptoms such as neck dysfunction and pain in patients with cervical spondylosis with neck syndrome. The addition of acupressure therapy significantly enhances the clinical efficacy of Tuina and functional exercise， with confirmed therapeutic benefits and good safety.关键词：Ringheaded thumb-tack needle;Neck-type cervical spondylosis;Tuina;Active Functional Exercise87|22|0更新时间：2025-06-21
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Research progress on the mechanisms of acupuncture in improving ischemic stroke through autophagy AI导读
“针刺治疗缺血性脑卒中与调节自噬密切相关，为临床治疗提供新思路和方案。”摘要：Stroke is a major disease with high incidence， disability rate， and mortality worldwide. In recent years， a large number of studies have comfirmed that acupuncture treatment for ischemic stroke is closely related to the regulation of autophagy mechanisms. This article summarizes the literature in recent years， discusses the effects of acupuncture on the degradation pathways mediated by autophagosomes， the expression of autophagy-related proteins， autophagy-related pathways， and the impact of mitophagy， and further explores the mechanisms of acupuncture in regulating autophagy to improve ischemic stroke， providing new ideas and schemes for clinical treatment.关键词：Ischemic stroke;Acupuncture;Autophagy;Mechanism;Review71|24|0更新时间：2025-06-21 -
Cerebral protective effect of acupuncture based on its regulative effect on programmed cell death AI导读
“据最新研究报道，针刺对大脑程序性细胞死亡影响显著，为脑病治疗提供新思路。”摘要：Programmed cell death （PCD） in the brain occurs throughout the whole process of life， and abnormal PCD may be an important factor leading to encephalopathy. It has been demonstrated that acupuncture has a good cerebral protective effect in the treatment of epilepsy， Alzheimer’s disease， Parkinson’s disease， depression， anxiety， insomnia， migraine and other dozens of brain diseases， and possesses characteristics of multi-point， networking， two-way and holistic regulation. In the present paper， we sum up the protective effect of acupuncture therapy from 1） cell apoptosis （for example， improving post-surgery cognition impairment， traumatic brain injury and ischemic stroke by relieving mitochondrial damage， activating mitochondrial autophagy， increasing blood-brain barrier permeability， and thus reducing cell apoptosis ）， 2） cell autophagy （for example， improving post-surgery cognition impairment， traumatic brain injury and ischemic stroke by relieving mitochondrial damage， activating mitochondrial autophagy， and increasing blood-brain barrier permeability， and thus reducing cell apoptosis）， 3） pyroptosis （for example， inhibiting abnormally-activated inflammasomes， and caspase and gasdermin D activity to remit pyroptosis in the treatment of stroke， depression， Parkinson’s disease， and Alzheimer’s disease animal models）， 4） ferroptosis （for instance， regulating iron ion metabolism， relieving lipid peroxidation damage， and thus playing a brain protection role in the treatment of ischemic and hemorrhagic stroke animal models）， 5） necroptosis （inhibiting necrotic apoptosis mediated by receptor-interacting protein kinase 1 in intracerebral hemorrhage rats）， and so on. We believe that along with the continuous development of brain science， artificial intelligence， big data and other new concepts and new technologies， it is expected to expand and improve the research level and quality of acupuncture in the prevention and treatment of encephalopathy through faster， more accurate and more comprehensive methods， which will help promote the modern interpretation of the brain protective effect of traditional acupuncture from the perspective of acupuncture regulation of PCD.关键词：Acupuncture;Encephalopathy;Programmed cell death;Cerebral protective effects108|22|0更新时间：2025-06-21 - “最新研究综述显示，针刺治疗哮喘可能通过调控免疫细胞因子等机制，抑制气道炎性反应，为哮喘治疗提供新思路。”摘要：Asthma is a chronic lung disease that occurs repeatedly and is hardly cured. Clinical studies have shown that acupuncture can effectively relieve asthma-related symptoms and is an ideal measure to treat asthma. The paper reviews the clinical and mechanism researches of acupuncture for asthma reported in China and other countries in recent 5 years， so as to provide the relevant evidences for the further study on asthma. The results showed that the mechanism of acupuncture in treatment of asthma may be related to modulating immune cytokines， ameliorating iron metabolism disorder， regulating the expression of neurotransmitter and receptor， promoting eosinophile apoptosis， inhibiting the expression of matrix metalloproteinase-9 and matrix metalloproteinase inhibitor-1， suppressing the expression of the related proteins and genes in type 2 innate lymphoid cells， inhibiting the signal pathways such as transforming growth factor-β1/Smad， mitogen-activated protein kinase， phosphatidylinositol 3-kinase/protein kinase B， signal transducer and activator of transcription， and nuclear factor-κB. Accordingly， the airway inflammatory response can be suppressed， the airway hyperreactivity be reduced， and the airway remodeling be relieved.关键词：Asthma;Acupuncture;Clinical trial;Mechanism research80|22|0更新时间：2025-06-21
- “针灸施术部位确定新原则“以知为数，以痛为输”，强调医生专业化认知和患者知痛部位判断，为经筋病治疗提供新方向。”摘要：The correct selection of the stimulated site is the key to the effectiveness of acupuncture and moxibustion. In the present paper， we propose that in the treatment of Jingjin （muscle， tendon， fascia， ligament， etc.） disease， the stimulated position of acupuncture or moxibustion should be determined by careful examination， rather than only selection of the fixed acupoints. The “Yi Zhi Wei Shu” （以知为数“Zhi”i.e. ”knowledge”）， means： the limit of the number of acupuncture or moxibustion treatment sessions is according to the therapeutic effect （improvement or cured） of a disease achieved， while the “Yi Tong Wei Shu” （“以痛为输”） refers to needling the site where the pain is， i.e. Ashi point， recorded in book Lingshu. Jingjin （Miraculous Pivot： Aponeurotic Structure along Meridians）， are the basic principles and methods for the treatment of Jingjin disease. The ancient physicians and modern doctors have different understandings on the selection of number of treatment sessions， and the stimulation of Ashi-points. Combining the meaning of “Zhi” of “Yi Zhi Wei Du” （“以知为度”meaning that the herb-medicines can be taken until the clinical condition has been improved to a certain extent， and it is not necessary to stop the medicines until it is completely cured） in Shang Han Za Bing Lun （Treatise on Febrile and Miscellaneous Diseases） for the first time， we hold that the “Zhi” of “Yi Zhi Wei Shu” reflects the doctors’ ability to grasp the rules of diagnosis and treatment of diseases， that is， doctors’ professional “cognition”. We also propose that the saying “Yi Zhi Wei Shu， Yi Tong Wei Shu” refers to guiding how to determine the stimulated spots of acupuncture or moxibustion for Jingjin disease. It is by no means to choose the pain site complained by the patient， but the doctor’s judgement is according to the patient’s perception of tenderness proposed during physical examination such as touching and pressing， which is the patient’s “Zhi”. The saying “Yi Zhi Wei Shu， Yi Tong Wei Shu” could be understood that the determination of the stimulated spots of acupuncture and moxibustion for Jingjin disease should be based on the doctor’s professional “cognition”. During the physical examination， the doctor makes a judgment on the patient’s “knowledge of pain site”， so as to determine the treatment points， that is， the “Zhi” of both doctors and patients should be achieved. Furthermore， combined with the theory of acupuncture and moxibustion in the treatment of Jingjin disease， tension-type headache， postherpetic neuralgia， primary trigeminal neuralgia and other diseases and heat-sensitive moxibustion theory， the connotation and clinical application value of “Yi Zhi Wei Shu， Yi Tong Wei Shu” are explained， in order to provide enlightenment for the determination of stimulated points of acupuncture and moxibustion.关键词：Acupuncture;Moxibustion;“Yi Zhi Wei Shu”;“Yi Tong Wei Shu”;Jingjin disease48|19|0更新时间：2025-06-21
- “在慢性阻塞性肺疾病治疗领域，研究者通过频次统计和复杂网络分析，探究了针药结合治疗的临床优势及选穴规律，为针药结合标准化治疗提供参考。”摘要：ObjectiveTo explore the clinical advantages and the rules of acupoint selection in treatment of chronic obstructive pulmonary disease （COPD） with the integration of acupuncture and medication by means of frequency statistics and complex network analysis， so as to provide references for the standardized treatment with the integration of acupuncture and medication.MethodSearched from PubMed， Web of Science， Embase， Ovid， CNKI， Wanfang， VIP， and SinoMed， the articles of clinical trials for COPD treated with the integration of acupuncture and medication were collected， dated from January 1， 2003 to September 1， 2023. Literature was screened according to the inclusion and exclusion criteria. Using frequency statistics， the clinical advantages of the integration of acupuncture and medication were explored. The database of acupoint prescriptions was established using Microsoft Excel 2019， the complex network model was constructed using SPSS18.0 and Gephi0.10， and the core acupoints and acupoint combinations were screened.ResultsIn the analysis of the clinical advantages of the combination of acupuncture and herbal medicine for the treatment of COPD， acupuncture alone contributed to the improvement of respiratory system indicators a total of 263 times， accounting for 51.77% of the total number of improved indicators， with the majority being improvements in resting lung function （184 times， 36.22%）. In addition， acupuncture alone also showed certain improvement effects on clinical symptoms， cardiac function， and biochemical blood indicators in COPD patients. The combination of acupuncture and traditional Chinese medicine （TCM） contributed to the improvement of respiratory system indicators 90 times， accounting for 42.45% of the total improvement frequency， with the primary focus being on improvements in resting lung function （66 times， 31.13%）， followed by improvements in clinical symptoms and biochemical blood indicators. The combination therapy for COPD mainly selected acupoints from the Bladder Meridian of Foot-Taiyang， with the combination of “Shenshu （BL23） + Feishu （BL13）” being the most frequently used acupoint pair. By performing “k-core” analysis and community network partitioning on the complex network diagram， three core acupoint clusters were identified.ConclusionThis article categorizes the clinical advantages of acupuncture and herbal medicine combination therapy for COPD into two types： the advantages of acupuncture alone and the advantages of acupuncture combined with TCM. Both can improve the function of target organs and enhance patients’ lung function. Moreover， for other non-target organ indicators， both also have an improvement effect， demonstrating the comprehensive regulatory capacity of the combination of acupuncture and herbal medicine. In the clinical acupoint selection for the treatment of COPD with the combination of acupuncture and herbal medicine， emphasis was placed on the application of specific acupoints， especially the use of confluent points. The selection is closely related to the pathogenesis of COPD， which is characterized by “fundamental deficiency and marked excess”. By combining “predominantly draining” acupoints with “predominantly tonifying” acupoints， the therapy aims to expel pathogenic factors while supporting the body’s healthy functions.关键词：Chronic obstructive pulmonary disease （COPD）;Integration of acupuncture and medication;Data mining;Rules of acupoint selection;Clinical advantages87|16|0更新时间：2025-06-21
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