最新刊期
卷 50 ， 期 3 ， 2025
- “最新研究发现，高强度电针能有效缓解大鼠内脏痛，抑制伤害性电活动，效果随强度增加而增强。”摘要：ObjectiveTo observe the analgesic effect of electroacupuncture （EA） at different intensities on visceral pain in rats and explore the supraspinal central mechanism and dose-response relationship.MethodsMale SD rats were randomly divided into a normal group， a model group and the groups of EA at different intensities （0.5， 1， 2， 3 and 6 mA， 6 rats/group）. The visceral pain rat model was established using mustard oil enema. Abdominal rectus electromyography （EMG） was used to observe visceral motor response in the rats. A colorectal distension （CRD） of 60 mm Hg was adopted to induce abdominal rectus muscle discharge for 20 s in each group. Afterwards， EA （0.5， 1， 2， 3， and 6 mA of intensity， 0.5 ms of pulse width， 10 Hz of frequency） was employed at “Zusanli” （ST36） for 1 min. After the end of EA， CRD was performed again for 20 s. Before and after EA， the areas under the curve （AUC） of CRD-induced abdominal rectus EMG were compared. Another set of rats were randomly divided into a model group and the groups of EA at different intensities （0.5， 1， 2， 3 and 6 mA， 8 rats/group）. Multi-channel in vivo electrophysiological technique was used to record the changes in electric activity of the subnucleus reticularis dorsalis （SRD） neurons in the medulla oblongate of rats， and SRD neural nociceptive electrical activity was observed 1 h after rectal mustard oil infusion. EA at different intensities was administered for 1 min at the 10-minute mark after rectal mustard oil infusion， and the changes in discharge frequency of nociceptive electrical activity of SRD neurons were compared before and after EA at different intensities. The proportion of SRD neurons whose discharge frequency decreased by more than 20% after EA was calculated.ResultsCompared to the normal group， the AUC of abdominal rectus EMG induced by 60 mm Hg CRD was increased in the model group and EA of different intensity groups before intervention （P<0.001）. After intervention， compared with the model group， AUC was decreased in the high-intensity （2， 3 and 6 mA） EA groups （P<0.05， P<0.001）. When compared with the low-intensity （0.5 and 1 mA） EA groups， AUC was reduced in the high-intensity EA groups （P<0.01， P<0.001， P<0.05）. In comparison with the indicators before modeling， the electrical activity of SRD neurons after rectal mustard oil infusion increased and remained excited and relatively stable in the duration from the 5-minute mark to the 30-minute mark in the rats （P<0.05）； and after high-intensity EA at 11-minute mark， the discharge frequency of SRD neurons was reduced （P<0.01， P<0.001）. Compared with the low-intensity EA， after the high-intensity EA， the reduced difference of discharge frequency increased （P<0.05，P<0.01， P<0.001）. The frequency difference， and the proportion of discharge frequency increasing over 20% after EA were elevated with the increase of EA intensity.ConclusionHigh-intensity EA can alleviate visceral pain and inhibit nociceptive electrical activity of SRD neurons in rats and the inhibitory effects are strengthened with the increase of EA intensity within a certain range.关键词：Electroacupuncture;Visceral pain;Stimulation intensity;Subnucleus reticularis dorsalis68|11|0更新时间：2025-06-21
- “最新研究发现，低强度电针“足三里”能改善小鼠神经炎性反应和认知功能，为治疗系统性炎性认知障碍提供新思路。”摘要：ObjectiveTo observe the effect of low-intensity electroacupuncture（EA） at “Zusanli”（ST36） on the hippocampal cholinergic anti-inflammatory pathway and α7 nicotinic acetylcholine receptor （α7nAChR） of hippocampal microglia in mice with systemic inflammatory cognitive impairment， so as to investigate the central mechanism of EA in improving neuroinflammatory response and cognitive function.MethodsC57BL/6 mice were randomly divided into blank， model and EA groups （6 mice/group）； another set of C57BL/6 mice were randomly divided into blank， model， EA and EA+antagonist groups（6 mice/group）； Cx3Cr1-Cre/ERT2 mice and α7nAChRfl/fl mice were mated to construct transgenic mice with microglial α7nAChR knockout （EA+ knockout group， 6 mice）， with the same litter negative mice used as non-knockout mice and randomly divided into blank， model+non-knockout and EA+non-knockout groups（6 mice/group）. The systemic inflammatory cognitive impairment model was established by intraperitoneal injection of lipopolysaccharide （LPS， 1 mg/kg）. Mice in the EA group received EA at bilateral ST36 （dense-sparse wave， 1 Hz/20 Hz， 0.5 mA）， for 30 minutes each time； mice in the EA+antagonist group received injection of α7nAChR antagonist methyllycaconitine citrate （MLA） into hippocampus. The episodic memory behavioral tests were used to observe the abilities of mice in new object exploration， object location change recognition and object temporal order change memory. Nuclear magnetic resonance spectroscopy was used to observe the choline level in the hippocampal tissue. Western blot was used to detect the protein expression of α7nAChR in the hippocampus. Immunofluorescence staining was used to detect the expression of α7nAChR in hippocampal microglia and the activation of microglia. Real-time quantitative PCR was used to detect the mRNA expression of tumor necrosis factor-α （TNF-α） in hippocampus， and ELISA was used to detect the contents of α7nAChR， inflammatory factors and microglial phenotypic markers in hippocampal tissue.ResultsCompared with the blank group， the abilities of new object exploration， location change recognition and object temporal order change memory， the choline level in the hippocampus， the protein expression level and content of α7nAChR and its co-expression with microglial markers， and the contents of anti-inflammatory factor interleukin （IL）-10 and M2 macrophage marker arginase 1 （Arg1） of mice in the model group were decreased （P<0.001， P<0.01）； while the expression of hippocampal ionized calcium-binding adapter molecule 1 （Iba-1）， the contents of M1 macrophage marker CD206， inducible nitric oxide synthase （iNOS）， the content of pro-inflammatory factor IL-1β and the mRNA expression of TNF-α were increased （P<0.001， P<0.01）. Compared with the model group， the above indexes in the EA group were reversed except for the ability of location change recognition （P<0.05， P<0.01， P<0.001）. Compared with the EA group， the abilities of new object exploration and object temporal order change memory in the EA+antagonist group were decreased （P<0.05）， and the mRNA expression of TNF-α was increased （P<0.01）； the abilities of new object exploration and object temporal order change memory in the EA+knockout group were decreased （P<0.05）， and the mRNA expression of TNF-α was increased （P<0.01）.ConclusionEA at ST36 with low-intensity may improve neuroinflammatory response and systemic inflammatory cognitive dysfunction by increasing the choline level in the hippocampus and reducing the release of inflammatory factors through up-regulating the expression of α7nAChR in hippocampal microglia.关键词：Electroacupuncture;Systemic inflammation;Cognitive impairment;Microglia;α7nAChR87|9|0更新时间：2025-06-21
- “最新研究发现，艾灸能显著提升血管性痴呆大鼠认知功能，可能通过调节海马区线粒体动力学相关蛋白发挥作用。”摘要：ObjectiveTo investigate the effect of moxibustion on hippocampal mitochondrial dynamics-related proteins in rats with vascular dementia （VD）， so as to reveal the underlying mechanisms of moxibustion in treating VD.MethodsSD rats were randomly divided into sham operation， model， moxibustion and medication groups， with 12 rats in each group. The VD model was prepared using an improved bilateral common carotid artery ligation method. Mild moxibstion was applied to “Shenting” （GV24）， “Baihui” （GV20） and “Dazhui” （GV14） for 20 min， once daily for 6 days per week. Rats of the medication group were treated with oral administration of nimodipine （12 mg/kg） once daily. All above interventions were performed for 4 weeks. Learning and memory abilities were assessed using Morris water maze test. Histopathological changes of hippocampus was observed with HE staining. Mitochondrial membrane potential （Δψm） and reactive oxygen species （ROS） levels in hippocampal tissue were measured by flow cytometry. The mRNA and protein expression levels of optic atrophy protein 1 （Opa1）， mitochondrial fusion protein 1 （Mfn1）， mitochondrial fusion protein 2 （Mfn2）， dynamics-related protein 1 （Drp1）， fission protein 1 （Fis1）， mitochondrial fission factor （Mff） in hippocampus was detected by PCR or Western blot， respectively.ResultsCompared with the sham operation group， the escape latency was prolonged （P<0.01）， the times of crossing the original platform were reduced （P<0.01）， the membrane potential of hippocampus， the mRNA and protein expression levels of Opa1， Mfn1 and Mfn2 in hippocampus were decreased （P<0.01， P<0.001） in the model group， while the average fluorescence intensity of ROS was increased， the mRNA and protein expression levels of Drp1， Fis1 and Mff were increased （P<0.001）. In comparison with the model group， the escape latency was significantly shortened （P<0.05）， the times of crossing the original platform were increased （P<0.05）， the membrane potential of the hippocampus， the mRNA and protein expression levels of Opa1， Mfn1 and Mfn2 were increased （P<0.05， P<0.001） in the moxibustion and medication groups， while the average fluorescence intensity of ROS in the hippocampus， the mRNA and protein expression levels of Drp1， Fis1 and Mff were decreased （P<0.001）. The average fluorescence intensity of ROS in the moxibustion group was significantly lower than that in the medication group （P<0.001）. HE staining showed loose arrangement of neurons， disappearance of partial nucleolus， and necrocytosis after modeling， which were relatively milder in both moxibustion and medication groups.ConclusionMoxibustion can effectively improve the cognitive function of VD rats， and its mechanism may be related to regulating hippocampal mitochondrial dynamics-related proteins， promoting mitochondrial fusion， inhibiting excessive mitochondrial division， thereby improving the imbalance of mitochondrial dynamics and alleviating mitochondrial dysfunction-related conditions.关键词：Vascular dementia;Moxibustion;Mitochondria;Mitochondrial dynamics;Fusion and fission78|7|0更新时间：2025-06-21
- “最新研究发现，针刺“解郁方”能有效改善抑郁大鼠行为，抑制炎症和氧化应激，可能与激活Nrf2有关。”摘要：ObjectiveTo observe the effect of acupuncture at “Baihui” （GV20）， “Shenmen” （HT7） and “Taichong” （LR3） （an acupoint prescription “Jieyufang” for treatment of depression） on inflammatory response and oxidative stress in depression rats induced by chronic unpredictable mild stress （CUMS）.MethodsForty male SD rats were randomly divided into control， model， medication and acupuncture groups， with 10 rats in each group. The depression model was established by CUMS stress stimulation for 28 days. Acupuncture was delivered at “Baihui” （GV20）， “Shenmen”（HT7） and “Taichong”（LR3） for 20 min， once a day for 28 days. Rats of the medication group received gavage of paroxetine hydrochloride （3.3 mg/kg） once a day for 28 days. Depressive behavior was evaluated by body weight， sucrose preference and tail suspension test. The contents of interleukin （IL）-6， IL-1β， IL-10 in serum， and the activities of superoxide dismutase （SOD）， catalase （CAT）， glutathione peroxidase （GSH-Px） and content of malondialdehyde （MDA） in hippocampus were detected by ELISA. H.E. staining was used to observe the morphological changes of hippocampus. The expression levels of nuclear factor-erythroid 2 related factor 2 （Nrf2） and Kelch-like epichlorohydrin-related protein factor 1 （keap1） in hippocampus were detected by Western blot.ResultsCompared with the control group， the body weight， sucrose preference index， contents of serum IL-10 and SOD， CAT and GSH-Px activities in the hippocampus， the expression level of Nrf2 protein were significantly decreased （P<0.05，P<0.01）； while the tail-suspension immobility time， contents of serum IL-6， IL-1β and MDA in hippocampus， the expression level of keap1 were increased （P<0.01） in the model group. Relevant to the model group， all the indexes mentioned above were reversed in both medication and acupuncture groups （P<0.05， P<0.01）. H.E. staining showed severe damage of hippocampal neurons， which was milder in medication and acupuncture groups.ConclusionEA of acupoint recipe “Jieyufang” can improve the depression-like behavior of CUMS rats， inhibit inflammatory response and oxidative stress， which may be related to its functions in activating Nrf2.关键词：Acupoint prescription “Jieyufang”;Depression;Inflammation;Oxidative stress80|8|0更新时间：2025-06-21
- “电针“天枢”“足三里”可改善溃疡性结肠炎小鼠症状，恢复结肠黏膜通透性，机制涉及调控NLRP3/Caspase-1/GSDMD轴抑制细胞焦亡，改善肠黏膜炎性损伤。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at “Tianshu” （ST25） and “Zusanli” （ST36） on NOD-like receptor protein 3（NLRP3）/cysteine aspartate specific protease 1 （Caspase-1）/gasdermin D （GSDMD） pyroptosis signaling pathway in ulcerative colitis （UC） mice， so as to explore its mechanism in improving UC by protecting intestinal barrier.MethodsFifty male C57BL/6 mice were randomly divided into normal， model， EA， sham acupuncture and medication groups， with 10 mice in each group. The UC mouse model was established by 3% DSS solution free drinking for 7 consecutive days. The mice in the EA group received EA （2 Hz/10 Hz， 0.2 mA） at bilateral ST25 and ST36 for 20 min， while the mice in the sham acupuncture group received only sham acupuncture （light and shallow acupunture at ST25 and ST36）. Mice in the medication group were orally administered mesalazine （33.4 g/kg） solution. All the interventions were performed once daily for a total of 7 days. The changes of body weight， stool shape and hematochezia of mice， the disease activity index （DAI） score， and the length of colon were recorded. The intestinal mucosal permeability was observed by in vivo small animal imaging system. HE staining was used to observe the pathological changes of colon tissue. TUNEL staining was used to observe the apoptosis of colon cells. The contents of interleukin （IL）-18 and IL-1β in serum were detected by ELISA. The average fluorescence intensity of tumor necrosis factor-α （TNF-α） and IL-6 in colon tissue was detected by immunofluorescence. The positive expression of IL-18 and IL-1β in colon tissue was detected by immunohistochemistry. The relative expression levels of zonula occludens-1 （ZO-1）， Occludin， NLRP3， Caspase-1 and GSDMD in colon tissue were detected by Western blot. The mRNA expressions of NLRP3， Caspase-1 and GSDMD in colon tissue were detected by qPCR.ResultsAfter modeling， the DAI， inflammatory infiltration， number of apoptotic cells， fluorescence intensity of TNF-α， IL-6 and FITC， positive expression rate of IL-18 and IL-1β， mRNA and protein expression levels of NLRP3， Caspase-1 and GSDMD， serum IL-18 and IL-1β contents were significantly increased （P<0.01） in the model group relevant to the normal group. At the same time， the colon length， ZO-1 and Occludin protein expression were significantly reduced （P<0.01）. The increased DAI， inflammatory infiltration， number of apoptotic cells， fluorescence intensity of TNF-α， IL-6 and FITC， positive rate of IL-18 and IL-1β， mRNA and protein expression levels of NLRP3， Caspase-1 and GSDMD， serum IL-18 and IL-1β contents， and the decreased colon length， ZO-1 and Occludin protein expression were all reversed after the EA and medication interventions compared with the model group and in the EA group than in the sham acupuncture group （P<0.01，P<0.05）.ConclusionEA can protect the intestinal mucosal permeability in UC mice， which may be related to its functions in regulating the NLRP3/Caspase-1/GSDMD axis and inhibiting pyroptosis， thereby alleviating the inflammatory injury of intestinal mucosa.关键词：Electroacupuncture;Ulcerative colitis;Pyroptosis;Intestinal barrier101|8|0更新时间：2025-06-21
- “最新研究发现，发酵白芥子贴能通过调节免疫平衡，降低炎症因子，有效治疗支气管哮喘。”摘要：ObjectiveTo observe the effects of fermented white mustard seed plaster on the balance of T helper cell （Th） 1/Th2， Th17/T regulatory cell （Treg） immune balance in bronchial asthma （BA） rats， so as to explore the possible mechanism of fermented white mustard seed plaster in the treatment of BA.MethodsHealthy SD rats were randomly divided into blank， model， medication and acupoint application groups， with 10 rats in each group. BA model was prepared using ovalbumin sensitization and atomization. Rats in the acupoint application group were treated with fermented white mustard seed plaster at “Dazhui” （GV14）， bilateral “Feishu” （BL13） and “Fengmen” （BL12） for 4 h； rats in the medication group were given intraperitoneal injection of 1 mg/kg dexamethasone sodium phosphate； both groups were treated once daily for 14 consecutive days. Behavioral observations and scoring were conducted on rats in each group before and after intervention. HE staining was performed to observe the pathological morphological changes of lung tissue. ELISA was used to detect the contents of interferon γ （IFN-γ）， interleukin （IL）-4， IL-17， IL-10， tumor necrosis factor α （TNF-α）， and immunoglobulin E （IgE） in serum. real-time quantitive PCR was used to detect the mRNA levels of T-box transcription factor （T-bet）， GATA binding protein 3 （GATA-3）， retinoic acid-related orphan receptor γt （RORγt）， and forkhead box protein 3 （Foxp3） in lung tissue. Western blot was used to detect the protein expression levels of T-bet， GATA-3， RORγt， and Foxp3 in lung tissue.ResultsAfter treatment， compared with the blank group， the behavioral score was significantly increased （P<0.01）； the thickness of bronchial wall muscle layer increased with a large amount of inflammatory cell infiltration， thickening of alveolar wall， abnormal lung tissue structure， and severe lung consolidation； the contents of serum IL-4， IL-17， TNF-α， and IgE were significantly increased （P<0.01）， while the contents of serum IFN-γ and IL-10 were significantly decreased （P<0.01）； the mRNA and protein expression levels of GATA-3 and RORγt in lung tissue were increased （P<0.01）， while the mRNA and protein expression levels of T-bet and Foxp3 in lung tissue were decreased （P<0.01） of rats in the model group. Compared with the model group， the behavioral score was significantly decreased （P<0.01）； the pathological morphological damage of lung tissue was significantly improved； the contents of serum IL-4， IL-17， TNF-α， and IgE were significantly decreased （P<0.01， P<0.05）， while the contents of serum IFN-γ and IL-10 were significantly increased （P<0.01， P<0.05）； the mRNA and protein expression levels of T-bet and Foxp3 in lung tissue were increased （P<0.01）， while the mRNA and protein expression levels of GATA-3 and RORγt in lung tissue were decreased （P<0.01） of rats in the medication and acupoint application groups. Compared with the medication group， the contents of serum IL-4 were significantly increased （P<0.05）； the mRNA and protein expression levels of GATA-3 and RORγt in lung tissue were significantly increased （P<0.01， P<0.05）， while the mRNA and protein expression levels of T-bet and Foxp3 were significantly decreased （P<0.01， P<0.05） of rats in the acupoint application group.ConclusionFermented white mustard seed plaster can alleviate airway inflammation in BA， and its mechanism may be achieved by up-regulating the expression of T-bet and Foxp3， down-regulating the expression of GATA-3 and RORγt， promoting Th1/Th2， Th17/Treg immune balance， and reducing the secretion of related pro-inflammatory factors to exert a therapeutic effect on asthma.关键词：Bronchial asthma;Fermented white mustard seed plaster;Airway inflammation;Th1/Th2， Th17/Treg100|13|0更新时间：2025-06-21
- “最新研究发现，线香灸疗法通过调节IL-6/STAT3通路，可减轻急性湿疹大鼠的炎症反应，促进Th17/Treg平衡恢复，使FLG和Caspase-14水平趋于正常。”摘要：ObjectiveTo investigate the effect of joss-stick moxibustion on the balance of helper T cell 17 （Th17）/regulatory T cell （Treg） through interleukin-6 （IL-6）/signal transduction and transcriptional activator 3 （STAT3） pathway in acute eczema （AE） rats.MethodsA total of 24 male SD rats were randomly divided into blank control， AE model， boric acid solution （BAS） washing， and moxibustion groups， with 6 rats in each group. The AE model was established by external application of 7% dinitrochlorobenzene （DNCB， 100 μL） to the abdominal skin once for the first day， 3 days later， the 5% DNCB to the right back sin， every three days for 3 times. Rats of the blank control group received external application of distilled water at the skin and those of the BAS washing group received external application of 3% BAS solution wet packing. Rats of the moxibustion group received local heat stimulation of joss-stick moxibustion at the injured skin locus for 30 min， once a day， 5 days a week， for 2 weeks. At the end of the experiments， the injured skin （on the right side of the back） and the healthy skin on the left side of the back were sampled using a round puncher （6 mm in diameter） for calculating the skin swelling degree. The inhibition rate equals to the skin swelling degree /weight of the healthy skin×100%. The content of serum IL-6 was detected using ELISA ， the expression levels of STAT3 and ROR-γt mRNAs in the injured skin tissue were detected using real-time fluorescence quantitative PCR， and the expression levels of p-STAT3， ROR-γt， and Foxp3 protein were detected using Western blot. The immunoactivities of filaggrin （FLG） and Caspase-14 in the injured skin tissue were detected by immunohistochemistry.ResultsCompared with the blank control group， the topical skin swelling degree， inhibition rate， serum IL-6 content， expression levels of STAT3 and ROR-γt mRNAs， and p-STAT3， ROR-γt protein expression levels were significantly increased （P<0.01， P<0.05）， while the expression level of Foxp3 protein and the immunoactivities of FLG and Caspase-14 were markedly down-regulated （P<0.01） in the model group. In comparison with the model group， both the increase and decrease of the aforementioned 10 indexes after the modeling were reversed in the BAS washing and moxibustion groups （P<0.01， P<0.05）. No significant differences were found between the BAS washing and moxibustion groups in the abovementioned 10 indexes.ConclusionJoss-stick moxibustion can reduce the inflammatory response in AE rats， which may be related to its functions in suppressing the overexpression of IL-6/STAT3 signaling pathway to promote the restoration of Th17/Treg balance， thus normalizing the levels of FLG and Caspase-14.关键词：Acute eczema;Joss-stick moxibustion;IL-6/STAT3;Th17/Treg63|8|0更新时间：2025-06-21
- “最新研究发现，电针“夹脊”能通过调节BDNF/NRF2信号通路，抑制氧化应激，保护背根神经节细胞，减轻神经疼痛，延缓腰椎间盘退变进展。”摘要：ObjectiveTo observe the protective function and mechanism of electroacupuncture （EA） “Jiaji” （EX-B2） on dorsal root ganglion （DRG） in rabbits with pressure-induced lumbar intervertebral disc degeneration （IVDD）.MethodsTwenty-five male New Zealand rabbits with mature bones were divided into control， sham operation， model， EA， and acupuncture groups， with 5 rabbits in each group. The IVDD model was established by inserting kirschner wires to the vertebral bone surface between the lumbar （L） 4 and L5 vertebrae， followed by applying continuous axial pressure for 28 days. EA （2 Hz/15 Hz， 1 mA） or acupuncture （only insertion of acupuncture needles into bilateral EX-B2， but without electrical stimulation） was applied to bilateral EX-B2 for 20 min， once daily for 28 days. The general conditions and weight changes of rabbits in each group were observed， and the free walking pain score was calculated after interventions. The morphology and structure of DRG cells were observed by HE staining. The activities of superoxide dismutase （SOD）， glycine peroxidase （GSH-Px） catalase （CAT） and content of malondialdehyde （MDA） in DRG were detected by ELISA. The concentration of calcium ions in DRG was measured by flow cytometry. The protein expression levels of brain-derived neurotrophic factor （BDNF）， nuclear factor E2 related factor 2 （NRF2）， heme oxygenase-1 （HO-1） and NAD （P） quinone oxidoreductase 1 （NQO1） in DRG were detected by Western blot.ResultsCompared with the sham operation group， the free walking pain score of rabbifs in the model group was significantly increased （P<0.01）， and the concentration of calcium ion and content of MDA in DRG were significantly increased （P<0.01）， the contents of SOD， GSH-PX and CAT and the protein expressions of BDNF， NRF2， NQO1 and HO-1 in DRG were significantly decreased （P<0.01）. Compared with the model group， the free walking pain score of rabbits in the EA group was significantly decreased （P<0.01）， the concentration of calcium ions and the content of MDA in DRG were decreased （P<0.01）， the contents of SOD， GSH-PX and CAT and the protein expressions of BDNF， NRF2， NQO1 and HO-1 were significantly increased （P<0.01）. The effect of EA was better than acupuncture （P<0.01， P<0.05）. HE staining showed atrophied cell bodies， disordered cellular arrangement， dissolved nuclei， and indistinct borders of DRG cells in the model group， which was milder in both acupuncture and EA groups.ConclusionEA at EX-B2 may inhibit oxidative stress in DRG cells by regulating BDNF/NRF2 signaling pathway， protect DRG cell damage， reduce nerve pain， thus delay the progression of IVDD.关键词：Electroacupuncture;Intervertebral disc degeneration;Dorsal root ganglion;Low back pain71|5|0更新时间：2025-06-21
- “最新研究发现，电针治疗能显著改善膝关节骨关节炎大鼠的疼痛症状，促进运动功能恢复，改善软骨组织病理形态，可能通过调节miR-335-5p表达、调控Wnt/β-catenin通路和抑制软骨基质相关降解酶释放发挥作用。”摘要：ObjectiveTo observe the protective effect of electroacupuncture （EA） on the knee joint cartilage tissue of rats with knee osteoarthritis （KOA）， so as to explore the potential mechanism of miR-335-5p involved in the treatment of EA for KOA.MethodsA total of 42 adult male Sprague-Dawley （SD） rats were randomly divided into blank， model and EA groups， with 14 rats in each group. The model was established by injecting a sodium glutamate iodoacetate solution （80 μg/μL） into the right joint cavity. On the 15th day after the model establishment， rats in the EA group received EA intervention at “Yanglingquan” （GB34） and “Xuehai” （SP10） on the right side， and the needles were retained for 15 minutes. The intervention was carried out every other day for a total of two weeks. After the two-week intervention， the paw withdrawal mechanical threshold （PWMT） and paw withdrawal thermal latency （PWTL） of the rats were detected； a gait analyzer was used to detect the gait adaptability changes induced by pain； the pathological changes of the knee joint cartilage tissue were observed after hematoxylin-eosin （HE） staining； qPCR and Western blot were used to detect the expression levels of miR-335-5p， Dickkopf related protein 1 （DKK-1）， β-catenin， and matrix metalloproteinase 13 （MMP-13） mRNAs and proteins in the cartilage tissue respectively； immunofluorescence staining was used to observe the positive fluorescence expression of β-catenin in the right knee joint cartilage tissue.ResultsIn the model group， HE staining showed a large area of chondrocyte necrosis， with the dissolution and disappearance of cell nuclei， accompanied by a macrophages-predominant inflammatory cell infiltration； cartilage surface defects in local areas， necrosis of trabecular bone and proliferation of fibrous tissue in local areas， and new capillary formation in the proliferative areas were also observed. In the EA group， pathological manifestations such as inflammatory cell infiltration and fibrous tissue proliferation were significantly alleviated. Compared with the blank group， the PWMT， PWTL， the support duration， footprint area， and average ground pressure of the right hind paw， and the mRNA and protein expressions of DKK-1 in the right knee joint cartilage tissue were decreased （P<0.001） in the model group， while the expressions of miR-335-5p， mRNA and protein expressions of β-catenin and MMP-13， and the fluorescence intensity of β-catenin in the right knee joint cartilage tissue were increased （P<0.001， P<0.01）. All the above indicators in the EA group were all reversed （P<0.001， P<0.05， P<0.01） in comparison with the model group.ConclusionEA can significantly improve the pain symptoms of KOA rats， promote the recovery of motor function， and improve the pathological morphology of the knee joint cartilage tissue. This may be related to regulating the expression of miR-355-5p and Wnt/β-catenin pathway， as well as inhibiting the release of cartilage extracellular matrix-related degrading enzymes.关键词：Electroacupuncture;Knee osteoarthritis;miR-335-5p;β-Catenin66|7|0更新时间：2025-06-21
- “最新研究发现，艾灸联合化疗药能显著抑制乳腺癌小鼠肿瘤生长，降低肿瘤组织中PD-1、TIM-3、CTLA-4的表达，为乳腺癌治疗提供新思路。”摘要：ObjectiveTo observe the effects of moxibustion combined with chemotherapy on immune checkpoints including programmed cell death protein 1 （PD-1）， T cell immunoglobulin domain and mucin domain （TIM-3） and cytotoxic T-lymphocyte-associated protein 4 （CTLA-4） in the tumor tissue of breast cancer-bearing mice， so as to explore the effect and mechanisms of moxibustion combined with chemotherapy on breast cancer.MethodsForty BALB/c female mice were randomly divided into model， chemotherapy， moxibustion and combination groups， with 10 mice in each group. 4T1 tumor cells were inoculated into the fat pad under the left fourth nipple of the mice to establish the breast cancer-bearing mice model. The chemotherapy group was injected intraperitoneally with doxorubicin （DOX） solution at 2.5 mg/kg once every 3 days； the moxibustion group received bilateral “Zusanli” （ST36） moxibustion with 2 moxa cones per point， treated every 2 days； the combination group received both DOX injection and moxibustion intervention. The above interventions lasted for 21 days. The body weight and tumor volume of the mice were recorded daily. HE staining was used to observe tumor tissue pathological morphology. Immunohistochemistry and Western blot were used to detect the positive expression and protein expression levels of PD-1， TIM-3， and CTLA-4 in the tumor tissue.ResultsCompared with the model group after intervention， the body weight was increased （P<0.01， P<0.05）， the tumor volume was decreased （P<0.01）， the pathological morphology of tumor tissue showed varying degrees of tumor cells degeneration， and the positive expression and protein expression levels of PD-1， TIM-3， and CTLA-4 in tumor tissue were decreased （P<0.01， P<0.05） of mice in the chemotherapy， moxibustion and combination groups. Compared with the chemotherapy group， the body weight of mice in the moxibustion group was higher （P<0.01）； the tumor volume of mice in the combination group was smaller （P<0.01）； the positive expression and protein expression levels of PD-1， TIM-3， and CTLA-4 were further reduced （P<0.01， P<0.05） of mice in the moxibustion and combination groups. Compared with the moxibustion group， the body weight was decreased （P<0.01）， the tumor volume was smaller （P<0.01）， the degree of tumor cell degeneration was higher， and the positive expression and protein expression levels of PD-1， TIM-3， and CTLA-4 were decreased （P<0.01） of mice in the combination group.ConclusionMoxibustion combined with chemotherapy shows more significant effects in inhibiting tumor growth in breast cancer-bearing mice compared to moxibustion or chemotherapy alone. It reduces the expression of PD-1， TIM-3， and CTLA-4 in tumor tissues， suggesting that lowering immune checkpoint expression levels may be one of the mechanisms by which moxibustion combined with chemotherapy drugs treats tumors.关键词：Moxibustion;Breast cancer;Immune Checkpoints;Programmed cell death protein 1 （PD-1）;T cell immunoglobulin domain and mucin domain （TIM-3）;Cytotoxic T-lymphocyte-associated protein 4 （CTLA-4）53|5|0更新时间：2025-06-21
- “最新研究显示，针刺治疗能显著提升贝尔氏面瘫患者面部肌力，缩短痊愈时间，为面瘫治疗提供新方案。”摘要：ObjectiveTo investigate the effects of acupuncture on the muscle strength and fatigue levels of facial muscles of the patients with Bell’s facial paralysis in the acute stage， using surface electromyography.MethodsSixty patients diagnosed with acute Bell’s facial paralysis were randomly assigned to an observation group （30 cases） and a control group （30 cases）. In the observation group， the routine acupuncture was applied to Quanliao （SI18）， Yangbai （GB14）， Yifeng （TE17）， Jiache （ST6） and Dicang （ST4） on the affected side and Hegu （LI4） on the healthy side. The needles were retained for 30 min， and acupuncture was delivered once every two days. In the control group， prednisone acetate combined with methylcobalamin tablets were administered orally. The interventions lasted till to the 28th day of illness in the two groups. At the baseline and after treatment， the root mean square amplitude （RMS） ratio and the median frequency （MF） of the frontal muscle， zygomatic muscle and orbicularis oculi muscle and the changes in the scores of H-B scale and Sunnybrook facial nerve function were observed in the two groups. The correlation was analyzed between the indicators detected by surface electromyography and the scale score， the clinical effect was compared and recovery time survival analysis was performed.ResultsThe RMS ratio and MF of the facial muscles （frontal， zygomatic， and orbicularis oculi muscles） were increased in both groups compared with pretreatment （P<0.01，P<0.05）， and RMS ratio in the observation group was higher than that of the control group after treatment （P<0.05）. After intervention， the H-B scale score and Sunnybrook score were improved in both groups compared with those pretreatment （P<0.01）， and the scores in the observation groups were superior to the control group （P<0.01）. Survival analysis of curative time showed that the curative time in the observation group was shorter than that of the control group （P<0.05）. After interventions， the cured and markedly effective rate of the observation group was 93.33% （28/30）， which was higher than that （76.67%， 23/30） of the control group （P<0.05）. Correlation analysis revealed that the H-B and Sunnybrook scores were negatively correlated with the RMS ratios of the frontal， zygomatic and orbicularis oculi muscles at the baseline and after treatment （P<0.01）； and the correlation was not presented with MF.ConclusionThe intervention with acupuncture in the acute stage of Bell’s palsy markedly increases the muscle strength of facial muscles， relieves muscular fatigue and shortens the curative time. The correlation analysis between the scale score and RMS ratios indicates that RMS ratios can be taken as one of the objective indicators in clinical assessment of the severity of facial paralysis.关键词：Bell’s palsy;Acute phase;Acupuncture therapy;Surface electromyography116|17|0更新时间：2025-06-21
- “在神经退行性疾病领域，针灸能有效减轻帕金森病症状，改善患者生活质量，为治疗帕金森病提供新方案。”摘要：Parkinson’s disease （PD） is a neurodegenerative disease commonly seen in middle-aged and elderly people. Acupuncture can effectively reduce the symptoms of PD and improve the life quality of patients. In this paper， we reviewed the relevant literature on acupuncture modulation of PD inflammation in recent years from the perspective of neuroinflammation. The results showed that the mechanism of acupuncture for inflammatory responses in treatment of PD is closely related to inhibiting the glial cell activation， regulating the peripheral immune cells， suppressing the release of inflammatory factors， inducing anti-inflammatory factor expression， modulating the expression of inflammatory signaling pathways， and attenuating the intestinal inflammation. It may be effective for alleviating neuroinflammation， reducing neuronal damage， and delaying the progression of PD. This paper provides the evidences and ideas for the deepening research of the mechanism of PD treated with acupuncture and moxibustion in the future.关键词：Acupuncture;Parkinson’s disease;Inflammation;Mechanism;Research progress77|7|0更新时间：2025-06-21
- “神经源性膀胱研究取得新进展，针灸干预机制被揭示，为治疗提供新思路。”摘要：The neurogenic bladder is a common manifestation of spinal cord injury. The occurrence and development of neurogenic bladder involves a variety of complex pathological mechanisms， including destruction of the structure and function of the detrusor muscles in the bladder tissue， abnormal excitatory conduction of bladder interstitial cells of Cajal， overactivation of the afferent C-fibers of the bladder， and loss of spinal cord neurons. It has been found that acupuncture is effective in the treatment of neurogenic bladder， and its underlying mechanisms are related to its functions in activating the bladder autophagy signaling pathway， modulating the expression of bladder fibroblasts molecules， regulating expression of proteins related to the contractile function of the detrusor muscles， stimulating excitatory conduction channel proteins on the Cajal interstitial cells of bladder， activating afferent C fibers within the bladder regulating spinal neuron regeneration， promoting the production of apoptotic factors in the spinal cord and bladder. In the present article， we summarized the research progress of the mechanism of neurogenic bladder after spinal cord injury and the mechanism of acupuncture and moxibustion interventions， so as to provide reference for elucidating the pathogenesis of neurogenic bladder and the role of acupuncture treatment.关键词：Acupuncture and moxibustion;Spinal cord injury;Neurogenic bladder;Mechanism;Review77|5|0更新时间：2025-06-21
- “针灸延缓衰老机制研究取得新进展，为应对人口老龄化问题提供新思路。”摘要：The aging of the population is one of the most concerns in the medical circle. Exploring the safe and effective anti-aging protocol is important in the study. The paper summarized the relevant articles for the anti-aging effect mechanism of acupuncture and moxibustion in recent 10 years from 9 aspects such as resisting oxidation and free radical damage， and regulating body immunity， neuroendocrine function， cell autophagy， aging-related gene expression， the deacetylase （Sirtuins） family， telomere with telomerase， epigenetic and iron metabolism. It is pointed out that acupuncture-moxibustion refers to the holistic therapy for anti-aging， characterized as multiple targets， multiple channels and full dimensions. The multidisciplinary cross-over study should be conduced on the basis of the dual regulatory effect of acupuncture and moxibustion， combined with modern life science and artificial intelligence technology， which is significant to reveal the scientific mechanism of acupuncture and moxibustion for anti-aging.关键词：Mechanism of aging;Anti-aging;Acupuncture and moxibustion101|17|0更新时间：2025-06-21
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