最新刊期
卷 50 ， 期 7 ， 2025
- “最新研究发现，腕踝针刺激能通过调节α7nAChR/JAK2/STAT3信号通路，改善化疗引起的神经痛。”摘要：ObjectiveTo investigate the effect of wrist-ankle acupuncture on the α7 nicotinic acetylcholine receptor （α7nAChR）/Janus kinase 2 （JAK2）/signal transducer and activator of transcription 3 （STAT3） signaling pathway in the spinal dorsal horn of mice with chemotherapy-induced neuropathic pain （CINP）， and to explore the mechanisms by which low-intensity wrist-ankle electroacupuncture alleviates pain hypersensitivity in CINP mice.MethodsC57BL/6 mice were randomly divided into a blank group， a model group， a wrist-ankle acupuncture group， and a wrist-ankle acupuncture + antagonist group， with 8 mice in each group. A CINP model was established by intraperitoneal injection of paclitaxel （2 mg/kg）. The wrist-ankle acupuncture group received electroacupuncture at the “Lower 4” and “Lower 5” points in the wrist-ankle region for 30 min per session， once every other day， for a total of 7 sessions. The wrist-ankle acupuncture + antagonist group received intrathecal injections of the α7nAChR antagonist α-BGT （0.5 μg/kg） on day 1， 7， and 13， in addition to acupuncture. The mechanical and thermal pain thresholds were assessed using Von Frey filaments and hot plate tests. The expression level of α7nAChR protein in the spinal dorsal horn （L4-L6） was determined by Western blot. Immunofluorescence staining was used to detect the expression of ionized calcium-binding adapter molecule 1 （Iba-1） in the spinal dorsal horn. ELISA was used to measure the relative levels of phosphorylated （p）-JAK2 and p-STAT3 in the spinal dorsal horn. Quantitative PCR was used to detect the relative mRNA expression levels of tumor necrosis factor-α （TNF-α）， interleukin-1β （IL-1β）， and interleukin-6 （IL-6）.ResultsCompared with the blank group， the model group exhibited decreased mechanical and thermal pain thresholds （P<0.001）， reduced α7nAChR protein expression （P<0.05）， increased Iba-1 expression （P<0.05）， elevated relative contents of p-JAK2 and p-STAT3 （P<0.05）， and increased mRNA expression levels of TNF-α， IL-1β， and IL-6 （P<0.05）. Compared with the model group， the wrist-ankle acupuncture group showed increased mechanical and thermal pain thresholds （P<0.05）， elevated α7nAChR protein expression （P<0.05）， decreased Iba-1 expression （P<0.05）， reduced relative contents of p-JAK2 and p-STAT3 （P<0.05）， and decreased mRNA expression levels of TNF-α， IL-1β， and IL-6 （P<0.05）. Compared with the wrist-ankle acupuncture group， the wrist-ankle acupuncture + antagonist group showed decreased mechanical and thermal pain thresholds （P<0.05）， lower α7nAChR protein expression （P<0.05）， increased Iba-1 expression （P<0.05）， higher relative contents of p-JAK2 and p-STAT3 （P<0.05）， and elevated mRNA expression levels of TNF-α， IL-1β， and IL-6 （P<0.05）.ConclusionWrist-ankle acupuncture may alleviate pain hypersensitivity in CINP mice by regulating the α7nAChR/JAK2/STAT3 signaling pathway in the spinal dorsal horn， inhibiting microglial activation， and reducing neuroinflammation.关键词：Wrist-ankle acupuncture;Electroacupuncture;Chemotherapy-induced neuropathic pain;α7 nicotinic acetylcholine receptor / Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway97|21|0更新时间：2025-07-16
- “最新研究发现，心肌缺血小鼠体表穴位敏化导致背根节神经元电信号改变，可能通过改变钠通道动力学特性介导体表穴位敏化的发生。”摘要：ObjectiveTo explore the relation between acupoint sensitization and alterations of electrical signals of dorsal root ganglion （DRG） neurons from the perspective of ion channel dynamics in myocardial ischemia （MI） model mice.MethodsSixty-four male C57BL/6J mice were randomly assigned to control and model groups （n=32/group）. The MI model was established by ligation of the left anterior descending （LAD） of the coronary artery. The MI was monitored by observing changes of ST segment of the standard limb-lead II of electrocardiogram （ECG-STII）. 2， 3， 5-triphenyltetrazolium chloride （TTC） and H.E. staining were used to evaluate the infarct area and histopathological changes in the myocardial tissue. Evans blue （EB） staining was administered via tail vein injection to observe the location， distribution， and quantity of exudate points on the body surface in both groups. Nociceptive changes were evaluated by measuring mechanical pain and thermal pain thresholds. Subsequently， electrophysiological assessments were conducted in vitro to evaluate whole-cell membrane currents， intrinsic excitability， and Na+ channel current variations in different types of DRG neurons.ResultsCompared with the control group， the ECG-STII segment in the model group was significantly elevated （P<0.000 1）， suggesting an induction of MI. TTC and H.E. staining showed a significant increase in the MI area （P<0.001）， accompanied by evident histopathological features such as myocardial fiber damage and inflammatory cell infiltration. The number of EB exudate points was significantly increased （P<0.01）， mainly distributing in the skin innervated by the T1—T5 spinal cord segments， as well as in the acupoints of “Feishu”（BL13）， “Jueyinshu”（BL14） and“Xinshu”（BL15）. Mechanical retraction reflex thresholds of the left upper and lower limbs of the model group significantly reduced （P<0.000 1）， and the thermal retraction latency of the left upper and lower limbs were notably shortened in the model group （P<0.01， P<0.000 1）， and the mechanical pressure pain threshold on the left dorsal skin significantly reduced （P<0.001）. The DRG medium-sized neurons displayed an obvious decrease in rheobase （P<0.05）， along with a notable increase in whole-cell membrane current， spike number， and average instantaneous frequency （P<0.05）， reflecting enhanced intrinsic excitability. The activation curves of TTX-R/TTX-S sodium channels shifted towards hyperpolarization （P<0.001）， while the inactivation curves moved towards depolarization （P<0.01）.ConclusionDRG medium-sized neurons in the T1—T5 spinal cord segments may influence the acupoint sensitization of the body surface by modifying their Na+ channel kinetic properties.关键词：Myocardial ischemia;Acupoint sensitization;Dorsal root ganglia;Na+ channel62|12|0更新时间：2025-07-16
- “最新研究发现，针刺可延长脑梗死大鼠溶栓时间窗，降低神经元铜死亡，为脑梗死治疗提供新思路。”摘要：ObjectiveTo observe the effect of acupuncture on neuronal cuproptosis after thrombolytic treatment in rats with cerebral infarction （CI）， so as to explore its underlying mechanisms in prolonging the time window of thrombolysis therapy for CI.MethodsIn the first part of the present study， 40 male SD rats were randomly divided into 5 groups： sham operation， model， 4.5 h thrombolysis， 6 h thrombolysis and acupuncture+6 h thrombolysis groups， with 8 rats in each group. The CI model was prepared by modified autologous thromboembolism method. Rats of the 4.5 h thrombolysis，6 h thrombolysis and acupuncture+6 h thrombolysis groups received thrombolysis with intravenous injection of recombinant human tissue plasminogen activator （rt-PA，10 mg/kg） through tail vein at 4.5 h or 6 h after modeling. Manual acupuncture stimulation was applied to bilateral “Neiguan”（PC6） and “Shuigou”（CV26） at the same time with thrombolysis administration for rats of the acupuncture+6 h thrombolysis group， with the needles retained for 30 min. The cerebral blood flow was monitored using laser diffusion blood flow imaging， and neurological deficit score was evaluated 2 h and 24 h after successful modeling using Bederson’s method. The CI volume was observed after triphenyltetrazole chloride staining. The immunoactivity of neuronal nuclei（NeuN） in the ischemic penumbra （IP） region of the cerebral cortex was detected by immunofluorescence staining. In the second part of the study， 24 male SD rats were randomly divided into sham operation group， model group， 6 h thrombolysis group and acupuncture+6 h thrombolysis group， with 6 rats in each group. The protein and mRNA relative expression levels of Ferredoxin-1 （FDX1） and dihydrolipoamide acetyltransferase （DLAT） in the IP region of the right cerebral cortex were detected by Western blot and real-time PCR， separately. The copper ion， pyruvate and adenosine triphosphate （ATP） contents in the IP area of the cerebral cortex tissue were detected using colorimetry.ResultsThe neurological deficit scores at both 2 h and 24 h after modeling， percentage of CI volume， the contents of copper ion and pyruvate， and the protein and mRNA expression levels of FDX1 and DLAT were significantly higher in the model group than those in the sham operation group （P<0.01）， while the NeuN positive expression and ATP content were evidently lower in the model group than those in the sham operation group （P<0.01）. In comparison with the model group， the neurological deficit score and percentage of CI volume in the 4.5 h thrombolysis and acupuncture+6 h thrombolysis groups， the contents of copper ion and pyruvate， and the protein and mRNA expression levels of FDX1 and DLAT in the acupuncture+6 h thrombolysis group， and the NeuN immunoactivity and ATP content in the 6 h thrombolysis group were significantly decreased （P<0.05， P<0.01）， while the immunoactivity of NeuN in both 4.5 h thrombolysis and acupuncture+6 h thrombolysis groups， ATP content in the acupuncture+6 h thrombolysis group， the percentage of CI volume， contents of copper ion and pyruvate， and the expression levels of FDX1 and DLAT proteins and mRNAs in the 6 h thrombolysis group were significantly increased （P<0.05， P<0.01）. Comparison among the 3 intervention groups showed that the neurological deficit score and percentage of CI volume were considerably lower in both of the 4.5 h thrombolysis and acupuncture+6 h thrombolysis groups than those in the 6 h thrombolysis group （P<0.01）， while the NeuN immunoactivity was considerably higher in both of the 4.5 h thrombolysis and acupuncture+6 h thrombolysis groups than in the 6 h thrombolysis group （P<0.01）. The contents of copper ion and pyruvate， and the protein and mRNA expression levels of FDX1 and DLAT were considerably lower in the acupuncture+6 h thrombolysis group than in the 6 h thrombolysis group （P<0.01）， while the ATP content was considerably higher in the acupuncture+6 h thrombolysis group than in the 6 h thrombolysis group （P<0.01）.ConclusionAcupuncture can prolong the time window of thrombolysis therapy for CI rats， which may be related to its function in down-regulating the protein and mRNA expressions of FDX1 and DLAT in ischemic cerebral cortex.关键词：Cerebral infarction;Acupuncture;Thrombolysis time window;Cuproptosis49|20|0更新时间：2025-07-16
- “最新研究发现，针刺能改善缺氧缺血性脑损伤新生大鼠运动控制能力，可能通过上调脑源性神经营养因子及其受体，激活相关信号通路，促进突触再生与重塑。”摘要：ObjectiveTo observe the effect of acupuncture on the expression of brain-derived neurotrophic factor （BDNF）， tyrosine kinase receptor B （TrkB）， extracellular signal-regulated kinase （ERK）， cyclic adenosine monophosphate （cAMP）-response element binding protein （CREB）， as well as synapsin1 （Syn1） and synaptophysin （Syp） in the striatum of neonatal rats with hypoxic-ischemic brain damage （ HIBD）， so as to explore its mechanisms underlying improvement of motor control.MethodsSeven-day-old neonatal SD rats were randomly and equally assigned to 4 groups： blank control， sham-operation， model， and acupuncture groups （n=12 per group） according to the randomized numerical table method. The HIBD model was established by using modified Rice’s method. For rats in the acupuncture group， manual acupuncture stimulation of “Dazhui”（GV14）， “Baihui”（GV20）， “Shuigou”（GV26） and “Mingmen”（GV4） was given 24 h after modeling. In the sham operation group， only the left common carotid artery was isolated without clamping， and no intervention was given. The neonatal rats in each group were subjected to the balance beam test 28 d after modeling. After the acupuncture intervention， the striatum tissue of neonatal rats in each group was taken for observing histopathological and morphological changes after H.E. staining. The ultrastructural changes of the neurons of striatum were observed by transmission electron microscopy， and the immunoactivity of Syp was detected by immunohistochemistry， and the expression levels of BDNF， TrkB， p-ERK， p-CREB， Syn1， and Syp proteins were detected by using Western blot.ResultsThere were no significant differences between the blank control and sham operation groups in the Longa score， balance beam test score， Syp immunoactivity， and expression levels of BDNF， TrkB， p-ERK， p-CREB， Syn1 and Syp proteins. Compared with the sham operation group， the balance beam test score at 28th day after modeling was significantly increased （P<0.01）， and the Syp immunoactivity and the expression levels of BDNF， TrkB， p-ERK， p-CREB， Syn1 and Syp proteins were considerably down-regulated （P<0.01， P<0.05， P<0.001） in the model group. In contrast to the model group， the balance beam test score at 28 d after modeling was obviously decreased （P<0.05）， the immunoactivity of Syp， and the expression levels of BDNF， TrkB， p-ERK， p-CREB， Syn1 and Syp proteins were strikingly up-regulated （P<0.05， P<0.01） in the acupuncture group. H.E. staining showed disordered arrangement of the striatal neurons， unclear layers， reduced cell density， with vacuolization and nuclear pyknosis in some cells in the model group， which was milder in the injury severity of the striatal neurons in the acupuncture group. Ultrastructure results displayed that in the model group， the distribution density of striatum synaptosome was lower， the thickness of postsynaptic dense area and the length of active area were reduced， and the boundary became blurred. Whereas in the acupuncture group， the synaptic structure of the striatum was more regular， the distribution density of synaptosomes， the thickness of the postsynaptic dense area and the length of the active area were increased.ConclusionManual acupuncture stimulation can improve the motor control ability in neonatal rats with HIBD， which may be achieved by up-regulating the endogenous BDNF and its receptor TrkB， activating the MAPK/ERK signaling pathway， promoting the phosphorylation of CREB， and up-regulating the expression of Syn1 and Syp proteins， thereby promoting synaptic regeneration and remodeling.关键词：Acupuncture;Hypoxic-ischemic brain injury;Striatum;Brain-derived neurotrophic factor;Tyrosine kinase receptor B;Synaptophysin67|18|0更新时间：2025-07-16
- “电针改善脑缺血再灌注损伤，抑制大脑皮层cGAS/STING/NLRP3通路表达，减轻炎性反应。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on cyclic GMP-AMP synthase （cGAS）/stimulator of interferon genes （STING）/NOD-like receptor family pyrin domain containing 3 （NLRP3） pathway of the cerebral cortex in rats with cerebral ischemia reperfusion injury and investigate the underlying mechanisms of EA in improving cerebral ischemia reperfusion injury.MethodsA total of 45 SD rats were randomly assigned into sham-operation group， model group and EA group， with 15 rats in each group. The modified thread embolism method was used to establish the model of cerebral ischemia reperfusion injury. Rats in the EA group received EA at “Baihui” （GV20）， “Fengfu” （GV16）， and “Dazhui” （GV14） for 20 min， once daily for 7 consecutive days. The modified neurological severity score （mNSS） was used to assess the neurological deficit condition. TTC staining was used to detect the percentage of cerebral infarction. HE staining was used to detect the pathological morphology in the cerebral cortex of infarcted side of rats. TUNEL staining was used to detect the apoptosis rate of cerebral cortical nerve cells. ELISA was used to detect the contents of interleukin （IL）-1β and IL-18 in the cerebral cortex of infarcted side. Western blot and qPCR were respectively used to detect the protein and mRNA expressions of cGAS， STING， TANK binding kinase-1（TBK1）， Interferon regulatory factor 3（IRF3） and NLRP3 in the cerebral cortex of infarcted side.ResultsIn comparison with the sham-operation group， the mNSS and the percentage of cerebral infarction were increased （P<0.01）； there were structural disorders and neuronal damage in the cerebral cortex of infarcted side； the apoptosis rate of nerve cells was increased （P<0.01）， the contents of IL-1β and IL-18 were increased （P<0.01）， and the protein and mRNA expressions of cGAS， STING， TBK1， IRF3 and NLRP3 were elevated （P<0.01） in the model group. In comparison with the model group， the mNSS and the percentage of cerebral infarction were decreased （P<0.01）； the pathological damage was alleviated； the apoptosis rate of nerve cells was decreased （P<0.01）， the contents of IL-1β and IL-18 were decreased （P<0.01）， and the protein and mRNA expressions of cGAS， STING， TBK1， IRF3 and NLRP3 were reduced （P<0.01） in the EA group.ConclusionEA can improve cerebral ischemia reperfusion injury， which is related to the inhibition of the cGAS/STING/NLRP3 pathway and inflammatory response in the cerebral cortex.关键词：Cerebral ischemia reperfusion injury;Electroacupuncture;cGAS/STING/NLRP3 pathway;Inflammatory response78|22|0更新时间：2025-07-16
- “最新研究发现，醒脑开窍针刺法通过调节AMPK/mTOR/ULK1信号通路，促进细胞自噬，改善缺血性脑卒中神经功能损伤，修复脑缺血再灌注损伤。”摘要：ObjectiveTo observe the effect of “Xingnao Kaiqiao” （regaining consciousness and opening orifice） acupuncture on cerebral ischemia/reperfusion injury（CI/RI）， autophagy and adenosine 5’-monophosphate-activated protein kinase （AMPK）/mammalian target of rapamycin （mTOR）/UNC-51-like autophagy-activated kinase 1 （ULK1） signaling pathway， so as to explore its underlying mechanisms in improving CI/RI.MethodsMale Sprague Dowley （SD） rats were randomly divided into sham-operation group， CI/RI model group， and acupuncture group， with 10 rats in each group. The CI/RI model was replicated by occlusion of the middle cerebral artery with intraluminal filament embolization. Two hours after successful modeling， the rats in the acupuncture group received manual acupuncture stimulation of “Shuigou” （GV26）， “Neiguan”（PC6）， and “Sanyinjiao” （SP6） with “Xingnao Kaiqiao” needling method， and electroacupuncture （EA） stimulation （2 Hz/15 Hz，1 mA， 20 min） of PC6 and SP6 on the affected side during needle retention. The intervention was conducted twice a day （10：00 and 16：00） for 7 times altogether. The severity of neurological deficits was assessed using Zausinger’s six-point method. TTC staining was used to detect the volume of cerebral infarction. H.E. staining was used to observe the histopathological and morphological changes of the infarct brain tissues. A transmission electron microscopy （TEM） was used to observe the neuronal ultrastructure and autophagosome formation in the hippocampal tissue. Western blot was used to detect the protein expression levels of microtubule-associated protein light chain 3-II （LC3-II）， AMPK， p-AMPK， mTOR， p-mTOR， ULK1 and p-ULK1 in the hippocampal tissues on the ischemic side.ResultsCompared with the sham-operation group， the model group had a significantly reduced neurological deficit score （P<0.001）， significantly decreased expression levels of LC3-II， ratios of p-AMPK/AMPK and p-ULK1/ULK1 （P<0.001）， and significantly increased percentage of cerebral infarct volume and p-mTOR/mTOR ratio （P<0.001）. In comparison with the model group， the acupuncture group had a significant increase in the neurological deficit score and expression of LC3-II， ratios of p-AMPK/AMPK and p-ULK1/ULK1 （P<0.001， P<0.05）， and a significant decrease in the percentage of cerebral infarct volume and p-mTOR/mTOR ratio （P<0.001）. Results of H.E. staining showed loose arrangement and reduction in the number of neurons， with pyknotic nucleus， obvious cavities， and appearance of degenerated and necrotic neurons in the ischemic brain tissue， which was relatively milder in the severity of CI/RI of the acupuncture group. Results of TEM showed injured and fractured membrane of neurons， moderate cell edema， mitochondrial swelling and reduction in number， dilation of the endoplasmic reticulum， and appearance of autophagosomes in the hippocampus of the model group， while in the acupuncture group， the nuclear membrane of nerve cells was relatively intact， the numbers of normal mitochondria and endoplasmic reticulum were increased， with appearance of typical autophagosomes.Conclusion“Xingnao Kaiqiao” acupuncture can improve neurological behavior and reduce infarction volume in rats with CI/RI， which may be related to its functions in regulating AMPK/mTOR/ULK1 signaling， and promoting cellular autophagy.关键词：Cerebral ischemia-reperfusion injury;“Xingnao Kaiqiao” acupuncture;Autophagy;AMPK/mTOR/ULK1 signaling pathway71|14|0更新时间：2025-07-16
- “最新研究发现，麦粒灸联合化疗能有效抑制乳腺癌小鼠肿瘤生长，可能通过抑制PI3K/AKT/mTOR信号通路，上调凋亡蛋白Caspase-3、Caspase-9表达。”摘要：ObjectiveTo observe the effect of wheat-grain moxibustion plus chemotherapy on the activities of phosphatidylinositol-3-kinase （PI3K）/protein kinase B （AKT）/mammalian target of rapamycin （mTOR） signaling and expressions of Caspase-3 and Caspase-9 in mice with breast cancer， so as to explore its mechanisms underlying suppression of tumor growth.MethodsForty female BALB/c mice were randomly divided into blank control， model， chemotherapy， wheat-grain moxibustion （moxibustion） and moxibustion+chemotherapy groups， with 8 mice in each group. The breast cancer model was established by injection of 4T1 cell suspension （0.1 mL） into the subcutaneous fat pad of the mouse’s right fourth nipple. After successful modeling， the mice of the chemotherapy group and moxibustion+chemotherapy group received injection of Adriamycin （2.5 mg/kg） into the mouse’ leg or abdomen in the morning of Monday and Friday every week， 3 weeks altogether. For mice of the moxibustion and moxibustion+chemotherapy groups， wheat-grain moxibustion was applied to “Shenque” （CV8） and bilateral “Zusanli” （ST36） for 2 cones every time， once every other day for 21 days. The body weight， tumor weight and tumor volume were measured. The histopathological changes of the tumor were observed after H.E. staining. The terminal-deoxynucleoitidyl transferase mediated nick end labeling （TUNEL） was used to detect the apoptosis of tumor tissues in each group. The expression levels of PI3K， AKT， mTOR， phosphorylated （p）-PI3K， p-AKT， p-mTOR， Caspase-3， Cleaved Caspase-3， Caspase-9， and Cleaved Caspase-9 proteins in the tumor tissues were determined by Western blot.ResultsCompared with the model group， the body weight in the chemotherapy group， the tumor weight， tumor volume， and the expression ratios of p-PI3K/PI3K， p-AKT/AKT and p-mTOR/mTOR in the chemotherapy， moxibustion and moxibustion+chemotherapy groups were significantly decreased （P<0.05）， while the apoptosis rate of tumor tissue， the expression levels of Caspase-3， cleaved Caspase-3， Caspase-9 and cleaved Caspase-9 proteins in the chemotherapy， moxibustion and moxibustion+chemotherapy groups were considerably increased （P<0.05）. The therapeutic effect of moxibustion + chemotherapy was significantly superior to that of simple chemotherapy and simple moxibustion in reducing the tumor volume and tumor mass， and in down-regulating the expression ratio of p-AKT/AKT （P<0.05）， and in up-regulating the apoptosis rate and the expression levels of Caspase-3 and cleaved Caspase-3 proteins （P<0.05）， and superior to that of simple moxibustion （not the simple chemotherapy） in up-regulating the expression levels of Caspase-9 and cleaved Caspase 9 protein （P<0.05）. The therapeutic effect of simple moxibustion was evidently inferior to that of simple chemotherapy in reducing tumor volume and tumor mass， in down-regulating the expression ratios of p-PI3K/PI3K and p-mTOR/mTOR （P<0.05）， and in increasing the apoptosis rate， and expression of Caspase-3， Caspase-9 and cleaved Caspase-9 proteins （P<0.05）. H.E. staining showed that the tumor cells of tumor tissue grew diffusely and arranged densely in the model group， while in the chemotherapy， moxibustion and moxibustion+chemotherapy groups， the tumor tissue showed light staining of cytoplasm and nucleus， reduced nuclear division， nuclear debris， smaller density， and different degrees of tumor cell necrosis， and the degree of necrosis was increased in the moxibustion group， chemotherapy group and moxibustion+ chemotherapy group.ConclusionMoxibustion plus chemotherapy and simple moxibustion can inhibit tumor growth in breast cancer mice， which may be related to their functions in inhibiting the expression and phosphorylation of PI3K/AKT/mTOR signaling pathway related proteins in the tumor tissue， and then upregulating the expression of apoptosis related proteins Caspase-3 and Caspase-9.关键词：Breast cancer;Wheat-grain moxibustion;Chemotherapy;PI3K/Akt/mTOR signaling pathway;Caspase-3;Caspase-955|13|0更新时间：2025-07-16
- “最新研究发现，经筋刺法能通过激活Wnt/β-catenin信号通路，促进膝关节骨关节炎大鼠骨骼肌再生修复。”摘要：ObjectiveTo observe the effects of the Jingjin（meridian-muscle region） needling on muscle growth markers， inflammatory factors， and proteins of Wnt/β-catenin signaling pathway in skeletal muscle of rats with knee osteoarthritis （KOA）， so as to explore its possible mechanisms in treating KOA.MethodsMale SD rats were randomly divided into three groups： blank group， model group， and Jingjin needling group， with 10 rats in each group. The KOA model was established using a knee joint immobilization method. In the Jingjin needling group， acupuncture was applied to the tendon insertion points of the rectus femoris muscle at the anterior thigh， the tendon insertion point of the rectus femoris muscle in the groin area， and spastic nodule of the soft tissue between the semitendinosus and biceps femoris long head at the ischial tuberosity on the right side， for 20 minutes per session， once a day， for a total of 14 days. Gait analysis technology was used to observe the effects of the acupuncture method on the behavior of rats. HE staining was used to observe the morphological structure of skeletal muscle. ELISA was used to detect the contents of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β） in skeletal muscle. Real time quantitative fluorescent PCR was utilized to measure mRNA expression levels of paired box protein 7 （Pax7）， myogenic differentiation antigen （MyoD）， myogenin （MyoG）， and myosin heavy chain-Ⅰ （MyHC1） in skeletal muscle. Western blot was used to assess the protein expression levels of β-catenin， glycogen synthase kinase 3β （GSK3β）， phosphorylated β-catenin （p-β-catenin）， and G1/S-specific cyclin D1 （CyclinD1） in skeletal muscle.ResultsThe model group exhibited damaged muscle fibers with noticeable disarray and infiltration of inflammatory cells； in contrast， the Jingjin needling group showed relatively intact muscle fiber structures and more orderly fiber arrangements， along with newly regenerated fibers. After acupuncture intervention， compared with the blank group， the model group showed decreased paw print area， maximum pressure， muscle wet weight， wet weight ratio， and hind limb circumference （P<0.01， P<0.05）； and increased contents of IL-1β and TNF-α in skeletal muscle （P<0.01）； and elevated mRNA expression of Pax7， MyoD， and MyoG （P<0.01）， while MyHC1 mRNA expression decreased （P<0.01）. The protein expressions of β-catenin and CyclinD1 in skeletal muscle were increased （P<0.05， P<0.01）， while those of GSK3β and p-β-catenin were decreased （P<0.05， P<0.01）. Compared to the model group， the Jingjin needling group rats demonstrated increased paw print area， maximum pressure， muscle wet weight， wet weight ratio， and hind limb circumference （P<0.01）； reduced contents of IL-1β and TNF-α in skeletal muscle （P<0.01， P<0.05）； and elevated mRNA expressions of Pax7， MyoD， MyoG， and MyHC1 （P<0.01）. Moreover， the protein expressions of β-catenin and CyclinD1 in skeletal muscle were increased （P<0.01）， while those of GSK3β and p-β-catenin were decreased （P<0.01）.ConclusionJingjin needling can promote the proliferation and differentiation of skeletal muscle satellite cells in KOA rats and exert regenerative and reparative effects by up-regulating the Wnt/β-catenin signaling pathway.关键词：Knee osteoarthritis;Jingjin;Acupuncture;Satellite cells;Muscle growth markers;Inflammatory factors;β-Catenin62|13|0更新时间：2025-07-16
- “在类风湿性关节炎治疗领域，研究者通过热补针法针刺“足三里”穴位，发现能有效调控Sirt1/FoxO1信号通路，减轻氧化应激损伤，为RA寒证治疗提供新方案。”摘要：ObjectiveTo observe the effects of heat-reinforcing needling on inflammation and oxidative stress in rheumatoid arthritis （RA） model rats with cold syndrome， so as to analyze the mechanism of heat-reinforcing needling in improving RA.MethodsMale SD rats were randomly divided into blank group， model group， heat-reinforcing needling group and resveratrol group according to random number table method， with 12 rats in each group. The rat model of RA with cold syndrome was established by subcutaneous injection of bovine type II collagen and complete Freund adjuvant combined with the stimulation of cold and wet environment. After successful modeling， rats of the resveratrol group was intraperitoneally injected with resveratrol （the agonist of Sirt1， 10 mg/kg）， and rats of the heat-reinforcing needling group received heat-reinforcing needling at “Zusanli”（ST36）for 30 min every day. After 14 d of continuous intervention， the synovial morphology of knee joint was observed by HE staining. The activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）， contents of reactive oxygen species （ROS） and malondialdehyde （MDA） in serum were detected by WST-1 method， colorimetric method probe method， and TBA method， respectively. The mRNA and protein expression levels of silencing information regulatory factor 1 （Sirt1） and （FoxO1） in the synovial tissue of knee joint were detected by Western blot and qPCR， respectively.ResultsCompared with blank group， the fibroplasia， inflammatory cell infiltration， the relative expressions of Sirt1 mRNA and protein in synovial of knee joint， serum SOD and GSH-Px activity in model group were significantly decreased（P<0.01）， while the relative expressions of FoxO1 mRNA and protein， the contents of ROS and MDA were significantly increased（P<0.01）. After interventions， the decreased Sirt1 mRNA and protein， decreased SOD and GSH-Px activities， and the increased FoxO1 mRNA and protein， increased contents of ROS and MDA were reversed （P<0.01，P<0.05）in both of the heat-reinforcing needling and resveratrol groups. At the same time， the fibrous tissue proliferation and inflammatory cell infiltration around the synovial tissue of knee joint induced by RA were significantly reduced in both of the intervention groups.ConclusionHeat-reinforcing needling can inhibit oxidative stress injury and inflammation response in RA rats with cold syndrome， which may be related to the regulation of Sirt1/FoxO1 pathway.关键词：Rheumatoid arthritis;heat-reinforcing needling;Oxidative stress;Sirt1/FoxO1 pathway52|14|0更新时间：2025-07-16
- “在老年衰弱患者腹腔镜结直肠癌手术中，经皮穴位电刺激内关、间使可改善术后恢复质量，降低不良反应发生率。”摘要：ObjectiveTo explore the effects of transcutaneous electrical acupoint stimulation （TEAS） at the bilateral acupoints Neiguan （PC6） and Jianshi （PC5） on heart rate variability （HRV） and inflammatory factors in frail elderly patients undergoing laparoscopic colorectal cancer surgery under general anesthesia.MethodsA total of 78 frail elderly patients undergoing elective laparoscopic colorectal cancer surgery were randomized into the TEAS group （39 patients， with 2 patients dropping out） and the control group （39 patients， with 1 patient dropping out）. In the TEAS group， TEAS was applied to the bilateral PC6 and PC5 from 30 min before anesthesia induction until the end of the surgery. The control group was connected to an electronic acupuncture instrument at the same acupoints but did not receive electrical stimulation. A short-term Holter electrocardiogram was used to collect HRV parameters in the frequency domain， including low-frequency power （LF）， high-frequency power （HF）， and the LF/HF ratio. Serum concentrations of C-reactive protein （CRP） and interleukin-6 （IL-6） were measured using ELISA. The Quality of Recovery-15 （Qor-15） score was recorded， along with adverse reactions such as postoperative nausea and vomiting （PONV）， palpitations， chest tightness， and chest pain.ResultsCompared with 1 d before surgery， LF and HF were significantly decreased （P<0.05）， and the LF/HF ratio significantly increased （P<0.05） at 1， 2， and 3 d after surgery in the control group. In the TEAS group， both LF and the LF/HF ratio were significantly decreased （P<0.05） at 1， 2， and 3 d after surgery. Compared with the control group， LF and HF levels were significantly increased （P<0.05）， and the LF/HF ratio was significantly decreased （P<0.05） in the TEAS group at 1， 2， and 3 d after surgery. Both groups exhibited increased levels of CRP and IL-6 at 1， 3， and 5 d after surgery compared to 1 d before surgery （P<0.05）， with the TEAS group showing lower CRP and IL-6 levels than the control group （P<0.05）. Compared to baseline before surgery， the Qor-15 scores of both groups were decreased at 1， 2， and 3 d after surgery （P<0.05）， with the TEAS group showing significantly higher Qor-15 scores than those in the control group （P<0.05）. The incidence of PONV， palpitations， chest tightness， and chest pain in the TEAS group was lower than that in the control group （P<0.05）.ConclusionTEAS at the PC6 and PC5 can regulate autonomic nervous function， reduce the early postoperative sympathetic nerve excitation， maintain parasympathetic nerve tension， reduce inflammatory responses， improve the quality of postoperative recovery， and decrease the incidence of postoperative adverse reactions in frail elderly patients after laparoscopic colorectal cancer surgery.关键词：Transcutaneous electrical acupoint stimulation;Elderly;Frailty;Autonomic nervous system;Heart rate variability68|65|0更新时间：2025-07-16
- “在骨关节炎治疗领域，专家通过针刀松解激痛点联合经筋推拿，显著改善患者症状，提高生活质量，可能与减轻炎性反应有关。”摘要：ObjectiveTo observe the clinical efficacy of acupotomy at trigger points combined with meridian sinew manipulation in treatment of early and mid-term knee osteoarthritis （KOA）， and investigate whether its therapeutic mechanism is related to the regulation of inflammatory response.MethodsSeventy-four patients with early and mid-term KOA were randomly divided into the control group （37 cases， with 2 cases dropouts） and the observation group （37 cases， with 1 case dropout）. The control group was treated with meridian sinew manipulation for 30 min each time， 5 times a week， for 4 consecutive weeks. The observation group was given acupotomy at trigger points on the basis of the treatment of control group， once a week， for 4 consecutive weeks. Western Ontario and McMaster University osteoarthritis index （WOMAC） scores， short-form McGill pain questionnaire （SF-MPQ） scores， the medical outcome 36-item short form health survey （SF-36） scores， serum inflammatory factors content before and after treatment were compared between the two groups. The clinical efficacy were assessed after the treatment.ResultsCompared with those before treatment， the total scores and the scores of pain， stiffness and activity impairment of WOMAC， the pain rating index （PRI）， visual analog scale （VAS） and present pain intensity （PPI） scores of SF-MPQ， and the contents of serum tumor necrosis factor （TNF）-α， interleukin （IL）-6 and IL-10 in both groups were significantly decreased after treatment （P<0.05）， while the scores of the physical component summary （PCS） and the mental component summary （MCS） of SF-36 were significantly increased （P<0.05）. Compared with the control group， the total scores， scores of pain， stiffness and activity impairment of WOMAC， the PRI， VAS and PPI scores of SF-MPQ， and the contents of serum TNF-α， IL-6 and IL-10 in the observation group were significantly decreased after treatment （P<0.05）， while the scores of PCS and MCS of SF-36 were significantly increased （P<0.05）. The total effective rate of the observation group was 94.4% （34/36） after treatment， which was significantly higher than that of the control group （74.3%， 26/35， P<0.05）.ConclusionAcupotomy at trigger points combined with meridian sinew manipulation can significantly improve the clinical symptoms such as joint pain， stiffness and limited activity in patients with KOA， improve the quality of life， and its mechanism may be related to reducing the inflammatory response.关键词：Knee osteoarthritis;Acupotomy;Trigger point;Randomized controlled trial;Clinical efficacy;Inflammatory response66|10|0更新时间：2025-07-16
- “针灸混合式系统评价（MMSR-A）综合分析针灸临床疗效及社会人文效应，为多学科跨文化针灸研究提供新方向。”摘要：The meta-analysis is generally employed to synthesize data in the conventional systematic review of acupuncture and moxibustion， in which， the quantitative analysis is predominant. Mixed methods systematic reviews of acupuncture （MMSR-A） adopts PICOS and SPIDER to formulate the questions； the research objects are expanded to qualitative and mixed studies. This mixed methods use the mixed methods appraisal tool （MMAT） for the methodology quality evaluation or the quality assessment tool of the Joanna Briggs Institue （JBI）， and a variety of mixed analytic methods. MMSR-A is applicable for the comprehensive evaluation of clinical efficacy and social/humanistic effects of acupuncture and moxibustion， for the organic synthesis of evidences from quantitative and qualitative studies of acupuncture and moxibustion， and for the systematic analysis in the multidisciplinary and cross-cultural field of acupuncture and moxibustion.关键词：Mixed methods systematic reviews （MMSR）;Acupuncture and moxibustion;Evidence-based medicine;Application54|10|0更新时间：2025-07-16
- “在膝关节骨关节炎治疗领域，艾灸显示出显著疗效，专家归纳了其抑制炎性反应、促进关节组织修复、调节免疫和肠道菌群的机制，为未来研究提供参考。”摘要：Knee osteoarthritis （KOA） is a common， highly disabling degenerative osteoarthropathy among middle-aged and elderly people. Moxibustion has been widely used in the treatment of KOA and exerts effects through multiple pathways with remarkable curative effects. This paper retrieved the relevant literature on moxibustion in the treatment of KOA at home and abroad in the past 10 years. After sorting and summarizing， it elaborated on the effective mechanisms of moxibustion from aspects such as inhibiting inflammatory responses， promoting the repair of joint tissues， regulating immunity and gut microbiota. Based on the current research， it also carried out thinking and prospects， aiming to provide references for relevant mechanism research in the future.关键词：Moxibustion;Knee osteoarthritis;Mechanism;Review68|13|0更新时间：2025-07-16
- “最新研究发现，铁死亡与中枢神经系统疾病密切相关，针刺干预机制丰富多样，为治疗提供新思路。”摘要：Central nervous system （CNS） diseases are complicated in the pathogenesis， unclear in mechanism， poor in prognosis and high in mortality. Ferroptosis is a new type of programmed cell death characterized by iron mediated lipid peroxidation. The studies in recent years have found that the pathological mechanism of the occurrence and development of various CNS diseases is closely related to ferroptosis of nerve cells. Acupuncture can intervene in CNS diseases by regulating ferroptosis of nerve cells. This paper introduces the exploration on the coordination of ferroptosis with the occurrence and development of CNS diseases and the effect mechanism of acupuncture. It is pointed out that the regulation of ferroptosis is an important mechanism of acupuncture on CNS diseases in view of multidimensional aspects， which is specially related to reducing brain iron load， alleviating oxidative stress， promoting the repair of mitochondrial function， and protecting neurons.关键词：Central nervous system disease;Acupuncture;Electroacupuncture;Ferroptosis;Mechanism70|23|0更新时间：2025-07-16
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