最新刊期
卷 50 ， 期 8 ， 2025
- “最新研究发现，电针刺激能通过激活中缝背核5-羟色胺能神经元-基底外侧杏仁核环路，有效缓解小鼠神经病理性疼痛和焦虑样行为。”摘要：ObjectiveThis study aims to investigate the intervention effects of electroacupuncture on neuropathic pain and anxiety-like behavior， and to explore the underlying mechanisms of the DRN5-HT-BLA （dorsal raphe nucleus serotoninergic - basolateral amygdala） neural circuit.MethodsMale C57BL/6J mice were selected， and a neuropathic pain model was established through spared nerve injury （SNI） surgery. Electroacupuncture （100 Hz， 0.3 mA， 30 min） was applied to bilateral “Zusanli” （ST36） and “Sanyinjiao”（SP6）. Anxiety-like behavior was assessed using the elevated plus maze （EPM） and open field （OF） tests， while mechanical allodynia was evaluated using the Von-Frey test. Viral tracing was used to confirm the anatomical connection between DRN5-HT and BLA. Immunofluorescence was used to assess the activity of 5-HT neurons in the DRN. Chemogenetic and calcium imaging techniques were employed to observe changes in the activity of 5-HT neurons in the DRN of DRN5-HT-BLA. Chemogenetics were used to observe the effect of inhibiting DRN5-HT-BLA on electroacupuncture.ResultsSNI model mice showed a significant decrease in mechanical pain threshold （P<0.000 1） and exhibited marked anxiety-like behavior in both the EPM and OF tests （P<0.01， P<0.05）. Compared to the model group， electroacupuncture significantly increased the mechanical pain threshold in the electroacupuncture group （P<0.000 1）. The EPM and OF results indicated that electroacupuncture significantly reduced anxiety-like behavior （P<0.05， P<0.001） without affecting spontaneous activity. Virus tracing results confirmed the anatomical connection between 5-HT neurons in the DRN and the BLA. Immunofluorescence results showed that the activity of 5-HT neurons in the DRN was significantly reduced （P<0.000 1） in SNI model mice， which was significantly reversed by electroacupuncture （P<0.01）. Calcium imaging revealed that in SNI model mice， the excitability of 5-HT neurons in the DRN5-HT-BLA circuit was decreased， and electroacupuncture treatment restored their excitability. After chemogenetic inhibition of the DRN5-HT-BLA circuit， electroacupuncture failed to improve mechanical allodynia and anxiety-like behavior in the SNI model mice （P<0.05，P<0.001，P<0.01）.Conclusion5-HT neurons in the DRN5-HT-BLA circuit play a crucial role in the mechanical allodynia and associated anxiety-like behavior in neuropathic pain. Electroacupuncture activates this circuit， enhancing the activity of 5-HT neurons in the DRN， thereby alleviating mechanical allodynia and anxiety-like behavior in SNI model mice.关键词：Neuropathic pain;Anxiety;Electroacupuncture;Dorsal raphe nucleus;basolateral amygdala;serotoninergic （5-HT） neurons34|13|0更新时间：2025-08-19
- “最新研究发现，舌下神经核神经元可直接投射至“廉泉”附近组织，参与电针“廉泉”改善中风后吞咽障碍的调控机制。”摘要：ObjectiveTo investigate the mechanisms by which the hypoglossal nucleus （12N） modulates swallowing-related activities and mediates the therapeutic effects of electroacupuncture （EA） at “Lianquan” （CV23） in alleviating post-stroke dysphagia （PSD）.MethodsTracer viruses were used to investigate the brain regions that directly project to CV23. C57BL/6J mice were divided into 7 groups： control， model， model+EA， normal+12N inhibition control， normal+12N inhibition， model+EA+12N inhibition control， and model+EA+12N inhibition groups （7 mice/group）. Three additional C57BL/6J mice were used for the virus tracing experiment. A photochemical thrombosis model was established in C57BL/6J mice to simulate PSD， by intraperitoneally injecting 0.2 mL of 1.5% rose bengal solution combined with laser irradiation to induce ischemic pathological changes in the primary motor cortex； EA at CV23 （2 Hz， 1 mA， for 15 minutes， once in total） to study the effect of electroacupuncture on swallowing function in PSD mice. Chemogenetic viral inhibition was applied to suppress neuronal activity in the 12N， and changes in vocal fold movements and swallowing electromyography were observed under physiological conditions， by laryngoscopy and electrophysiological recorder respectively. After PSD model establishment， EA combined with chemogenetic inhibition of 12N was followed to observe the effect of chemogenetic inhibition of 12N on EA at CV23 in improving PSD. Tracer viruses were used to trace the upper brain region of the 12N.ResultsThe 12N directly projected to the surrounding tissues of CV23. Compared with the control group， in the model group， food residues were observed in the pharyngeal cavity and piriform recesses， along with prolonged vocal cord movement cycle time， a reduced number of vocal cord movement cycles within 10 seconds （P<0.01）， and decreased electromyographic area under the curve as well as reduced number of swallows （P<0.001， P<0.01）. Compared with the model group， the model+EA group showed shortened vocal cord movement cycle time， an increased number of vocal cord movement cycles within 10 seconds （P<0.01）， and an enlarged electromyographic area under the curve （P<0.001）. Physiologically， compared with the normal+12N inhibition control group， the normal+12N inhibition group showed a reduction in the area under the swallowing electromyography curve and a decrease in the number of swallows （P<0.05）. Pathologically， laryngoscopy showed that compared with the model+EA+12N inhibition control group， the vocal fold movement cycle time of the model+EA+12N inhibition group was prolonged， and the number of movement cycles within 10 seconds was reduced （P<0.01）， the area under the swallowing electromyography curve was decreased （P<0.05）. The upstream brain regions of the 12N projecting to the surrounding tissues of CV23 were mainly identified in the nucleus tractus solitarii （NTS）， intermediate reticular nucleus （IRt）， spinal trigeminal nucleus caudalis （SP5C） and lateral paragigantocellular nucleus （LPGi）， etc.ConclusionThe 12N directly projects to the surrounding tissues of CV23 and contributes to EA-mediated improvement in PSD.关键词：Post-stroke dysphagia;Hypoglossal nuclei;“Lianquan”（CV23）;Virus tracing27|26|0更新时间：2025-08-19
- “最新研究发现，电针双侧“天枢”能改善溃疡性结肠炎小鼠的炎性反应，可能与激活骶副交感节后神经元相关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） of bilateral “Tianshu” （ST25） on ulcerative colitis （UC） in mice， and further investigate its related neural circuits including colonic-associated sympathetic and sacral parasympathetic postganglionic components.MethodsThirty C57BL/6J male mice were randomly divided into normal control， model and EA groups， with 10 mice in each group. The UC model was established by free access to 3% dextran sulfate sodium （DSS） solution for 7 consecutive days. Under isoflurane inhalation anesthesia on day 1， 3， 5， and 7 of modeling， mice of the EA group received EA stimulation of bilateral ST25 （2 mA， 10 Hz） for 30 min each time. The colonic histopathological changes were assessed by HE staining， and the body weight of mice was recorded. The relative abundance values of acetylcholine （ACh） and norepinephrine （NE） in the distal colon tissues were detected by using high performance liquid chromatography-mass spectrometry. In addition， six male C57BL/6J mice were randomly and equally divided into model and EA groups. On the fifth day of modeling， mice in the EA group received once EA stimulation with the same operations above， while mice in the model group were only anesthestized without EA. Immunofluorescence staining was used to examine the expression of c-Fos protein of the tyrosine hydroxylase-positive （TH+） neurons in the superior mesenteric ganglion （SMG）， inferior mesenteric ganglion （IMG）， and vesicular acetylcholine transporter-positive （VAChT+） neurons in the pelvic ganglion （PG）.ResultsFrom day 7 to 9 of DSS modeling， the model group showed significant decrease of body weight compared to the normal control group （P<0.001）， and the EA group showed a significantly lower reduction in body weight in contrast to the model group （P<0.01， P<0.05）. The histopathological score （thickening of the colon wall， injury of the lamina propria structure， submucosal edema， and inflammatory cell infiltration） was significantly increased after modeling （P<0.000 1） and obviously improved after EA （P<0.001）. Compared with the normal group， the relative abundance of ACh in the distal colon of the model group decreased significantly （P<0.05），which increased significantly in the EA group （P<0.05） when compared with the model group. No significant differences were found among the 3 groups in the relative abundance value of NE in the distal colonic tissues. Results of immunofluorescence staining showed that， compared with the model group， there were no statistically significant differences in the expression of c-Fos in TH+ neurons of SMG and IMG （sympathetic postganglionic component innervating the colon tissue） in the EA group. However， compared with the model group， the number of VAChT+ neurons that express c-Fos in PG of the EA group increased significantly （P<0.05）.ConclusionEA of bilateral ST25 can ameliorate the inflammatory response and histopathological injury in UC mice， which may be achieved by activating the sacral parasympathetic postganglionic neurons in the PG instead of sympathetic postganglionic components.关键词：Electroacupuncture;“Tianshu” （ST25）;Ulcerative colitis;Neuroimmune regulation;Pelvic ganglion32|9|0更新时间：2025-08-19
- “最新研究发现，不同强度电针对肌肉炎性痛状态下小鼠背根神经节神经元钙活动具有差异性镇痛效应，高强度电针效果更显著。”摘要：ObjectiveTo observe the differential analgesic effects of electroacupuncture （EA） at different intensities on calcium activity in dorsal root ganglia （DRG） neurons induced by mechanical， thermal， and cold stimuli in a state of muscular inflammatory pain， so as to preliminarily reveal the peripheral integration characteristics of acupuncture analgesia mechanisms.MethodsThe inflammatory muscle pain model was established by injection of complete Freund’s adjuvant （CFA） into the right tibialis anterior muscle. EA （0.5 mA or 1 mA， 2 Hz） was applied to “Zusanli” （ST36） on the right side for 10 min. Before and after the EA， mice were given body surface stimulation of brush， clamp and different temperature， while simultaneously recording DRG neuron calcium activity during stimulation. The effects of different stimulation methods before and after EA on the change in fluorescence intensity （ΔF/F0） and the percentage of activated DRG neurons were compared.ResultsIn the clamp hind paw stimulation， both 0.5 mA and 1 mA EA could reduce the fluorescence intensity of neurons， and the effect of 1 mA was better than that of 0.5 mA （P<0.001）. Specifically， 0.5 mA EA decreased the percentage of medium and large neurons （P<0.05）， while 1 mA EA decreased the percentage of small， medium， and large neurons （P<0.05， P<0.01）， and the effect of 1 mA was better than that of 0.5 mA in reducing the percentage of small and medium neurons （P<0.01）. In the stimulation of clamping tibialis anterior muscle， both EA intensities could reduce the fluorescence intensity of neurons （P<0.05， P<0.000 1）， and the effect of 1 mA was better than that of 0.5 mA （P<0.000 1）， and 1 mA EA could reduce the percentage of small and medium neurons （P<0.05）. In the brush stimulation， both EA intensities could reduce the fluorescence intensity of neurons （P<0.000 1）， and the effect of 1 mA was better than that of 0.5 mA （P<0.000 1）， and 0.5 mA EA decreased the percentage of medium and large neurons （P<0.05）， while 1 mA EA decreased the percentage of small， medium and large neurons （P<0.05， P<0.01）. However， in the temperature stimulation （43， 51， 4 ℃）， there were no obvious changes in the fluorescence intensity and the percentage of activated neurons in different intensities of EA.ConclusionBoth 0.5 mA and 1 mA EA intervention at ST36 can inhibit the reactivity of DRG neurons to mechanical stimulation， with higher intensity EA showing more significant effects.关键词：Electroacupuncture;dorsal root ganglia neurons;Analgesic circuit;Calcium imaging;Inflammatory muscle pain41|37|0更新时间：2025-08-19
- “最新研究揭示针刺通过激活脑区、功能连接和突触传递三个维度调控神经环路，为针灸治疗疼痛、情绪障碍等提供新思路。”摘要：An increasing number of studies indicate that the modulation effect of acupuncture on intracerebral neural circuits is a crucial pathway through which acupuncture exerts its therapeutic effects. Among these， brain region activation， functional connectivity， and synaptic transmission collectively form the three dimensions of acupuncture modulation of neural circuits. Brain region activation reflects the activity state of key nodes in neural circuits， functional connectivity focuses on the intensity and patterns of information exchange within neural circuits， and snaptic transmission examines molecular-level changes during signal projection in neural circuits. To systematically elucidate the mechanisms of acupuncture， this paper first analyzed the impact of acupuncture on these three dimensions using commonly used points such as Quchi （LI11） as examples. Then， it explored the multi-dimensional regulatory effects of acupuncture on neural circuits related to clinically advantageous conditions like pain， mood disorders， and gastrointestinal dysfunction. Finally， it proposed issues and potential solutions for the multi-dimensional analysis of acupuncture-related neural circuits， aiming to provide insights for advancing research on acupuncture mechanisms and guiding further precision treatment.关键词：Acupuncture;Neural circuits;Brain region activation;Functional connectivity;Synaptic transmission42|36|0更新时间：2025-08-19
- “岛叶皮层研究揭示腧穴-脏腑相关性，为穴位调节提供神经学依据，开辟新研究方向。”摘要：The insular cortex contains corresponding somatosensory region and visceral motor region， which respectively process somatic and visceral information. Through short-range fiber projections， it can integrate somatosensory information and modulate visceral functions. The insular cortex has a close connection with the autonomic nervous system and is a key hub for regulating the autonomic nervous system， making it a potential target for revealing the correlation between acupoints and internal organs. The unique “visceral localization” characteristics and the spatial distribution of neuronal functions in the insular cortex provide a neurological basis for elucidating the “multi-effects of one acupoint” or “bidirectional regulation” of the acupoints. Future researches should focus on the targeted regulation of different acupoints through different regions of the insular cortex， and further analyze the regulatory effect between acupoints and internal organs through the insular-autonomic nerve-target organ reflex pathway.关键词：Insular cortex;Specific effects of acupoints;Multi-effects of one acupoint;Autonomic nervous system;Bidirectional regulation27|9|0更新时间：2025-08-19
- “针刺通过重塑神经环路改善卒中后神经功能损伤，为卒中康复提供新思路。”摘要：Stroke is a loss of brain functions due to disturbance of blood supply， leading to a series of diseases and sequelae. Nerve circuit damage is an important cause of functional impairment after stroke. The neural circuit in the brain is a system of interconnected neurons， and is the only way for information transmission in the human body. Neural plasticity and circuit reconstruction are thought to contribute to post-stroke recovery. Acupuncture can effectively improve neural function damage after stroke by remodeling the neural circuit. In the present paper， we summarized the mechanisms of acupuncture in promoting the restoration of motor， sensory， cognitive and other functions after stroke from： 1） increasing the excitability of motor cortex， enhancing the corresponding functional connectivity between the premotor cortex/adjacent supplementary motor area and the supramarginal gyrus （intercortical circuit）； 2） enhancing the effective connection between the cerebellum and sensorimotor cortex （cortico-cerebellum circuit）； 3） regulating the enhanced functional connectivity between the cortex and the ipsilateral basal ganglia and the weakened functional connectivity between the cortex and the contralateral basal ganglia （cortico-basal ganglia circuit）； and 4） promoting the functional connectivity between the cortex and hippocampus， strengthening hippocampal nerve regeneration and regulating synaptic plasticity （cortico-hippocampal circuits）. In summary， current studies have focused on the regulation of acupuncture on the neural circuit between the cerebral cortex and other brain regions and on in vivo imaging mechanisms. However， the specific regulation mechanism of acupuncture on single neural circuit and whether there is a correlation between each circuit need to be further studied.关键词：Acupuncture;Stroke;Cerebral cortex;Neural circuit;Functional imaging studies;Review22|10|0更新时间：2025-08-19
- “最新研究发现，电针“肝俞”能改善小鼠生物钟紊乱和血糖异常，可能通过调节IGF1介导的PI3K/Akt通路发挥作用。”摘要：ObjectiveTo investigate the mechanism of electroacupuncture （EA） underlying improvement of abnormal blood glucose in mice with disturbance of peripheral biological clock.MethodsC57BL/6J mice （half male and half female） were randomly divided into normal control （n=26）， model （n=26）， EA （n=26） and EA+ inhibitor （n=8） groups. The circadian rhythm disturbance model was established by subjecting the mice to constant light （12-h light-light ［LL］ cycle） for 4 weeks. EA （4 Hz/20 Hz， 0.2 mA） was applied to bilateral “Ganshu” （BL18） for 15 min， once daily for 8 weeks. The mice of the EA+inhibitor group were given LY294002 （40 μmol/L， 10 μL） by intraperitoneal injection for blocking activities of phosphatidylinositol-3-kinase （PI3K） signaling pathway， once every other day for 8 weeks. After the intervention， the fasting plasma glucose （FPG） was measured， and fasting serum insulin （FINS） and the circadian rhythm of liver insulin-like growth factor 1 （IGF1） were observed by measuring their contents with ELISA. The insulin resistance was evaluated by homeostasis model assessment of insulin resistance （HOMA-IR） and quantitative insulin sensitivity check index （QUICKI）. The histopathological changes of the liver tissue were observed by H.E. staining. The changes of liver glycogen granule deposition were observed by PAS staining. The immunoactivity of forkhead box protein O1 （FoxO1） and glycogen synthase kinase 3β（GSK3β） in the liver tissue was detected by immunofluorescence technique. The expression levels of IGF1， IGF1R， FoxO1， GSK3β， phosphoenolpyruvate carboxykinase （PEPCK） and glucose 6 phosphatase（G6Pase） mRNA and protein kinase B （Akt） and phosphorylated （p）-Akt proteins in the liver tissue were detected by qPCR and Western blot， respectively.ResultsIn comparison with the normal control group， daily water intake， the contents of FPG， FINS and HOMA-IR， the immunoactivity of GSK3β and FoxO1 in liver tissue， and the expression levels of IGF1R， GSK3β， FoxO1， PEPCK and G6Pase mRNA in liver tissue were significantly increased （P<0.01， P<0.05）， while the serum QUICKI， ratio of p-PI3K/PI3K and p-Akt/Akt， and expression of IGF1 mRNA in liver tissue obviously decreased （P<0.01） of mice in the model group. Compared with the model group， all the above indicators were significantly reversed in the EA group （P<0.05， P<0.01）. After administration of the inhibitor LY294002 of PI3K signaling， the effects of EA in up-regulating the ratio of p-PI3K/PI3K and p-Akt/Akt， and in down-regulating the immunoactivity of GSK3β and FoxO1， and the expression levels of GSK3β， FoxO1 and G6Pase mRNA in liver tissue were eliminated （P<0.05）. H.E. staining showed irregular arrangement of the hepatocytes with diffuse swelling， loose connections between hepatocytes， fat vacuoles of different sizes in the cytoplasm， and diffuse steatosis in some mice of the model group. PAS staining showed disordered arrangement of hepatocytes， with a large number of fat vacuoles， and relatively thin and uneven staining between and within cells in the model group. These situations were evidently improved in the EA group and EA+ inhibitor group， including reduction in the arrangement of liver cells and the vacuoles of fat in cytoplasm.ConclusionAcupuncture of BL18 can not only improve the disordered circadian rhythm， but also lower the abnormally elevated blood glucose in mice with disturbance of circadian rhythm， which may be related to its functions in activating IGF1-mediated PI3K/Akt signaling， thereby inhibiting the mRNA and protein expression of GSK3β， FoxO1， PEPCK and G6Pase， and improving liver glucose metabolism.关键词：Electroacupuncture;Ganshu （BL18）;Circadian rhythm;Blood glucose;Insulin-like growth factor 1 （IGF1）23|5|0更新时间：2025-08-19
- “最新研究发现，电针治疗能减轻纤维肌痛综合征大鼠的疼痛，降低脊髓背角P物质表达，抑制星形胶质细胞活化，促进5-HT表达，减轻中枢及外周炎性反应。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on mast cell （MC） activity， substance P （SP） and glial fibrillary acidic protein （GFAP） expression in the lumbar spinal dorsal horns （SDHs） and related inflammatory factors in the anterior tibial muscle， lumbar spinal cord and serum of fibromyalgia syndrome （FMS） model rats， so as to explore its mechanisms underlying improvement of FMS.MethodsThirty-six male SD rats were randomly divided into blank control， model and EA groups， with 12 rats in each group. The FMS model was established by injecting 2-morphine phenol ethanol sulfate into the left anterior tibial muscle. After successful modeling， EA was applied to “Zusanli”（ST36） and “Yanglingquan”（GB34） on the left hind limb for 20 min， once every other day for a total of 7 times. von Frey test was used to detect the mechanical pain threshold （MPT） of both hind limbs. H.E. staining was used to observe morphological changes of the anterior tibial muscle. Immunofluorescence staining was used to determine the expressions of SP， MC， GFAP and 5-hydroxtryptamine （5-HT） in the lumbar SDHs. Suspension chip method was used to detect the contents of inflammatory factors in the lumbar spinal cord， left anterior tibial muscle and serum.ResultsCompared with the blank control group， the difference value of MPT between the bilateral hind-paws， immunofluorescence-positive number of SP and GFAP in the SDHs， contents of TNF-α， macrophage inflammatory protein-1α （MIP-1α）， monocyte chemoattractant protein-1 （MCP-1） in the lumbar spinal cord， and the contents of MIP-α， MCP-1， interleukin （IL）-1α， granulocyte-macrophage colony-stimulating factor （GM-CSF） and granulocyte-colony stimulating factor （G-CSF） in the left anterior tibial muscle， as well as TNF-α， IL-1β， GM-CSF and 5-HT contents in the serum were significantly increased （P<0.05）， while the immunofluorescence-positive number of 5-HT and contents of 5-HT and IL-10 in the lumbar spinal cord， and IL-10 content in the left anterior tibial muscle and serum were strikingly decreased （P<0.05） in the model group. In comparison with the model group， the increase of the difference value of MPT between the bilateral hind-paws， immunofluorescence-positive number of SP and GFAP， contents of TNF-α， MIP-α and MCP-1 in the lumbar spinal cord， and the contents of MIP-α， MCP-1， IL-1α， GM-CSF and G-CSF in the left anterior tibial muscle， as well as the contents of TNF-α， IL-1β， GM-CSF and 5-HT in the serum， and the decrease of the immunofluorescence-positive number of 5-HT and contents of 5-HT and IL-10 in the lumbar spinal cord， and IL-10 content in the left anterior tibial muscle and serum were reversed in the EA group （P<0.05）. Immunofluorescence staining displayed that no apparent changes in the number of MC in the lumbar SDHs after modeling and EA， and MCs presented degranulation in both the model group and EA group. H.E. staining showed disordered arrangement of muscle fibers， enlargement of the gaps between the muscle fibers， and obvious inflammatory infiltration of the anterior tibial muscle in the model group. Compared with the model group， the morphology of muscle fibers in the EA group was relatively complete， with orderly arrangement and reduction of the inflammatory infiltration.ConclusionEA of ST36 and GB34 can relieve pain and reduce the central and peripheral inflammatory response in FMS rats， which may be related to its functions in suppressing the expressions of SP and GFAP， and astrocytes activation in the lumbar SDHs and up-regulating the expression of 5-HT in the lumbar spinal cord.关键词：Electroacupuncture;Fibromyalgia syndrome;Dorsal horn of spinal cord;Inflammatory response26|62|0更新时间：2025-08-19
- “最新研究发现，艾灸能减轻动脉粥样硬化小鼠血管内皮损伤，改善内皮功能，促进内皮修复，其作用机制可能与调控SIRT1/eNOS信号通路有关。”摘要：ObjectiveTo observe the effect of moxibustion on pathological structure and ultrastructural changes of thoracic aorta， the expression of silent information regulator 1 （SIRT1） and endothelial nitric oxide synthase （eNOS） in ApoE-/- atherosclerosis （AS） mice， so as to explore its possible mechanism in preventing and treating AS.MethodsTen C57BL/6J mice were fed a normal diet as the control group， and 30 ApoE-/- mice were fed a high-fat diet to establish the AS model， which were randomly divided into the model group， moxibustion group， and moxibustion+EX527 group， with 10 mice in each group. Mice in the moxibustion group received mild moxibustion treatment at “Danzhong”（CV17）， “Shenque”（CV8）， “Neiguan”（PC6， bilateral）， and “Xuehai” （SP10， bilateral） for 30 min， the mice in the moxibustion+EX527 group were given intraperitoneal injection of EX527 （10 mg/kg） 30 min before moxibustion， with both treatments given once daily， 5 times a week， with a total intervention period of 12 weeks. After the intervention， the contents of nitric oxide （NO） and endothelin-1 （ET-1） in serum were detected by ELISA. HE staining and transmission electron microscopy （TEM） were used to observe the pathological structure and ultrastructural changes of thoracic aorta. The expression of endomucin of thoracic aorta was observed by immunofluorescence staining. The protein and mRNA expression levels of SIRT1 and eNOS in thoracic aorta were detected by Western blot and real-time fluorescence quantitative PCR， respectively.ResultsCompared with the control group， the content of serum NO was decreased （P<0.05）， while the content of serum ET-1 was increased （P<0.05）； the fluorescence intensity of endomucin， the protein and mRNA expression of SIRT1 and eNOS in thoracic aorta were significantly decreased （P<0.05） in the model group. Compared with the model and moxibustion+EX527 groups， the moxibustion group showed a significantly increase in the serum NO contents （P<0.05）， and decrease in serum ET-1 contents （P<0.05）， and the endomucin fluorescence intensity， SIRT1 and eNOS protein and mRNA were significantly increased （P<0.05）. Morphological observation revealed that in the model group， light microscope showed incomplete thoracic aorta structure， uneven inner wall， endothelial cell degeneration and swelling， and TEM showed a few swollen mitochondria and autophagy， and a large number of dilated rough endoplasmic reticulum， and these situations were obviously milder in the moxibustion group.ConclusionMoxibustion can reduce vascular endothelial injury， improve endothelial function and promote endothelial repair in AS mice， which may be related to its function in regulating the SIRT1/eNOS signaling pathway.关键词：Atherosclerosis;Moxibustion;SIRT1;eNOS;Endothelial function18|4|0更新时间：2025-08-19
- “最新研究发现，艾灸通过激活PI3K/AKT/mTOR信号通路，降低肠易激综合征大鼠肠上皮细胞自噬水平，保护肠黏膜屏障。”摘要：ObjectiveTo observe the effect of moxibustion on visceral hypersensitivity and the phosphatidylinositol-3 kinase （PI3K） /protein kinase B （AKT） /mammalian target of rapamycin （mTOR） signaling pathway in rats with diarrhea-predominant irritable bowel syndrome （IBS-D）， so as to explore its mechanisms underlying amelioration of pain reaction of IBS-D by regulating the autophagy level of intestinal epithelial cells and protecting the intestinal mucosal barrier.MethodsForty male SD rats were randomly divided into control， model， moxibustion and moxibustion+LY294002 groups， with 10 rats in each group. The IBS-D model was established by acetic acid enema + chronic binding method. In the moxibustion group， the rats were given suspended moxibustion on bilateral “Tianshu” （ST25） and “Shangjuxu” （ST37） for 20 minutes. Rats of the moxibustion+LY294002 group received intraperitoneal injection of PI3K inhibitor LY294002 （0.75 mg/kg） 30 min before each moxibustion intervention. Both groups were treated once a day for 7 days. The minimum colon-rectal water injection volume （minimum water-injection volume） for achieving a score of 3 points of the abdominal withdrawal reflex （AWR） was measured to assess the rats’ visceral pain reaction before and after modeling， and after the intervention. H.E. staining was used to observe inflammatory damage of the colonic mucosa. A transmission electron microscopy was employed to examine the mitochondrial structure and the number of autophagosomes in the colon mucosa. The expressions of Occludin， ZO-1， Claudin-2， p62 proteins， and the ratios of p-PI3K/PI3K， p-AKT/AKT， p-mTOR/mTOR and LC3B-Ⅱ/Ⅰ were detected by Western blot. The mRNA expression levels of PI3K， AKT， mTOR， Occludin， ZO-1 and Claudin-2 in the colon tissues were detected by qPCR.ResultsCompared with the control group， the minimum water injection volume， and the expression levels of p-PI3K/PI3K， p-AKT/AKT， p-mTOR/mTOR ratios， Occludin， ZO-1 and p62 proteins and the mRNA expression of PI3K， AKT， mTOR， Occludin and ZO-1 were significantly decreased （P<0.01）， while the protein and mRNA expression level of Claudin-2 and the ratio of LC3B-Ⅱ/Ⅰ were strikingly increased in the model group （P<0.01）. H.E. staining showed disordered arrangement of the intestinal epithelial cells， and infiltration of local inflammatory cells， and electron microscopy observation showed multiple autophagosomes in the model group. In comparison with the model group， the minimum water injection volume，the p-PI3K/PI3K， p-AKT/AKT， p-mTOR/mTOR ratios， the protein expression levels of Occludin， ZO-1 and p62 and the mRNA expressions of PI3K， AKT， mTOR， Occludin and ZO-1 in the colon tissue were considerably increased （P<0.05，P<0.01）， while the protein and mRNA expression of Claudin-2 and the ratio of LC3B-Ⅱ/Ⅰ were apparently decreased in the moxibustion group （P<0.01）. After administration of PI3K inhibitor， the above effects of moxibustion were eliminated （P<0.01，P<0.05）. In the moxibustion group， the intestinal mucosal structure was relatively intact， inflammatory cell infiltration was decreased， and the quantity of autophagosomes under the electron microscope was reduced， while in the moxibustion+LY294002 group， the intestinal mucosal structure was relatively disordered， the inflammatory cell infiltration was increased， and the quantity of autophagosomes was increased.ConclusionMoxibustion can relieve visceral pain of IBS-D rats， which may be related to its functions in alleviating intestinal epithelial damage and protecting the intestinal mucosal barrier by activating the PI3K/AKT/mTOR signaling pathway and reducing the level of autophagy of the intestinal epithelial cells.关键词：Diarrhea-predominant irritable bowel syndrome;Moxibustion;Visceral pain;PI3K/AKT/mTOR signaling pathway;Intestinal mucosal barrier;Autophagy26|14|0更新时间：2025-08-19
- “最新研究发现，电针治疗能显著改善变应性鼻炎引起的嗅觉障碍，可能通过抑制炎症因子损伤嗅觉上皮和神经元发挥作用。”摘要：ObjectiveTo observe the effects of electroacupuncture （EA） on olfactory function， olfactory epithelial cell apoptosis， olfactory marker protein （OMP）， cysteinyl aspartate-specific protease-3 （Caspase-3）， and the expression of inflammatory factors in rats with allergic rhinitis （AR）-induced olfactory dysfunction， so as to explore the underlying mechanism by which EA improves olfactory function in AR-induced olfactory dysfunction.MethodsSD rats were randomly divided into control， model and EA groups， with 8 rats in each group. AR-induced olfactory dysfunction rats model was established using ovalbumin sensitization. Bilateral "Yingxiang" （LI20） acupoints were stimulated with EA （2 Hz/15 Hz， 1 mA） for 10 min， once daily for 14 days. After the intervention， AR symptom scores of each group of rats were evaluated. Olfactory function was assessed using the buried food pellet test. HE staining was performed to observe the morphological changes in olfactory mucosa tissue. ELISA was used to detect plasma IgE， interleukin-1β （IL-1β） and tumor necrosis factor-α （TNF-α） contents in plasma and nasal lavage fluid. Immunofluorescence staining was used to detect OMP and Caspase-3 positive expressions in olfactory mucosa. TUNEL staining was performed to detect olfactory epithelial cell apoptosis condition.ResultsCompared with the control group， rats in the model group showed significantly thinner olfactory mucosal epithelium， reduced number and disorganized arrangement of olfactory receptor neurons （ORNs）， increased inflammatory cell infiltration， significantly decreased olfactory function， significantly decreased OMP expression in olfactory mucosa （P<0.01）， significantly increased nasal symptom scores， plasma IgE contents， IL-1β and TNF-α contents in plasma and nasal lavage fluid， significantly increased Caspase-3 expression in olfactory mucosa （P<0.01）， and significantly increased occurrence of olfactory epithelial cell apoptosis （P<0.01）. Compared with the model group， rats in the EA group showed significantly thicker olfactory mucosal epithelium， increased number and more orderly arrangement of ORNs， reduced inflammatory cell infiltration， significantly improved olfactory function， significantly increased OMP expression in olfactory mucosa （P<0.01，P<0.05）， significantly decreased nasal symptom scores， plasma IgE contents， IL-1β and TNF-α contents in plasma and nasal lavage fluid （P<0.05，P<0.01）， significantly decreased Caspase-3 expression in olfactory mucosa （P<0.01）， and significantly decreased occurrences of olfactory epithelial cell apoptosis （P<0.01）.ConclusionEA therapy can improve olfactory function of olfactory-malfunctioned AR rat. The mechanism could be inhibition of the AR-induced damage of inflammatory cytokines to olfactory epithelium and neurons.关键词：Allergic rhinitis;Olfactory dysfunction;Electroacupuncture;Olfactory marker protein;Cysteinyl aspartate-specific protease-321|8|0更新时间：2025-08-19
- “在失眠症治疗领域，专家建立了基于XGBoost的疗效预测模型，为针刺治疗提供辅助决策工具。”摘要：ObjectiveTo establish a predictive model of acupuncture treatment of insomnia and to create a profile of suitable populations for acupuncture schemes， so as to help improve clinical efficacy.MethodsThe data was sourced from a prospective clinical study on acupuncture treatment of insomnia by “Tongdu Tiaowei” acupoint prescription （Baihui ［GV20］， Yintang ［EX-HN3］， bilateral Shenmai ［BL62］ and bilateral Zhaohai ［KI6］）. Data from 113 insomnia patients were included in the analysis of the present study， with the reduction rate of the Pittsburgh Sleep Quality Index （PSQI） served as the overall clinical efficacy evaluation. First， the feature selection was performed using univariate logistic regression and Boruta algorithm， and the prediction accuracy of the three boosting algorithms — adaptive boosting， gradient boosting， and extreme gradient boosting （XGBoost） — was compared for selecting the best algorithm. The grid search and ten-fold cross-validation were used to optimize the hyperparameters of the best algorithm. The optimal dataset partitioning method was selected using stratified random partitioning， and the best cut-off value was determined based on the Youden index. The predictive model for the therapeutic efficacy was constructed and its performance was evaluated. Finally， SHAP （shapley additive explanation） analysis was used to visually interpret the model.ResultsThe features included in the model were the proportion of stage N1 to total sleep duration， the proportion of stage N2 to total sleep duration， R latency from lights out， stage N2 latency from lights out， the awake time after sleep onset， PSQI sleep efficiency score， and the presence of an old tongue （a tongue picture of a dry， rough texture and an old body）. XGBoost was identified as the best algorithm， with the optimal probability threshold of 0.76， a corresponding precision of 0.91， a recall of 0.91， a F1 score of 0.91， an accuracy of 0.91， and an area under curve （AUC） of 0.82. Patients who meet the following conditions are more likely to respond to “Tongdu Tiaowei” acuoint stimulation： the proportion of N1 phase was about 6%—70% of the total sleep duration， N2 phase latency was less than about 40 min from the time when the lights were off， the wakefulness time was less than about 75 min or 100—300 min after falling asleep， the R phase latency was more than about 75 min from the time when the lights were off. The N2 phase were about 20%—50% of the total sleep duration， PSQI sleep efficiency score was 2 or 3， and there was no appearance of “old tongue”.ConclusionThe predictive model of the efficacy of acupuncture treatment for insomnia established using XGBoost， along with the preliminary profile of the suitable population constructed using SHAP， provides a reliable auxiliary decision-making tool for acupuncture treatment of insomnia.关键词：Insomnia;Acupuncture;Machine learning;Predictive Model;Auxiliary Decision-Making26|25|0更新时间：2025-08-19
- “最新研究发现，针刺通过调节PI3K/AKT信号通路改善阿尔茨海默病。”摘要：Acupuncture has a definite effect on the treatment of Alzheimer’s disease （AD）， and its molecular mechanism has been studied more and more deeply. The phosphatidylinositol 3-kinase/protein kinase B （PI3K/AKT） signaling pathway is mainly involved in the regulation of cell proliferation， apoptosis， autophagy and synaptic plasticity， and plays an important role in the occurrence and development of AD. In this paper， we reviewed the role of PI3K/AKT pathway in the pathogenesis of AD and the mechanism of acupuncture intervention， and concluded that the abnormal expression of PI3K/AKT signaling pathway can lead to imbalance of cellular oxidative defense system， impairment of mitochondrial structure and function synaptic destruction， abnormal autophagy and energy metabolism disorders， and acupuncture can improve oxidative stress damage， enhance synaptic plasticity， regulate autophagic activity and regulate energy metabolism by regulating PI3K/AKT signaling pathway to cure AD.关键词：Acupuncture;Alzheimer’s disease;Phosphatidylinositol 3-kinase/protein kinase B signaling pathway;Molecular mechanisms23|13|0更新时间：2025-08-19
- “针灸治疗急慢性荨麻疹研究取得新进展，专家分析针灸处方特点，为临床治疗提供依据，弥补单一治疗不足，发挥针灸中药协同增效。”摘要：ObjectiveTo analyze the characteristics of point prescriptions for acute and chronic urticaria treated with acupuncture using complex network and data mining， so as to provide the basis for acupoint selection and the approaches to clinical treatment of acute and chronic urticaria.MethodsThe clinical trials of acupuncture in treatment of urticaria were systematically searched from China National Knowledge Infrastructure （CNKI）， VIP database （VIP）， Wanfang database （WanFang）， SinoMed and PubMed. According to the screening criteria， the “database of acupuncture for acute and chronic urticaria” was established. IBM SPSS Modeler18.0， Cytoscape3.9.0， Origin2021， Gephi0.9.2 and other softwares were used for frequency analysis， association rule analysis and cluster analysis.ResultsA total of 352 articles were included， and 158 acupuncture prescriptions were extracted， involving 76 points. The analysis focused on main acupoints， and the laws of acupoint selection and medications based on syndrome differentiation. The results showed that the meridian with the highest use frequency of acupuncture was the bladder meridian of foot-taiyang. The top 6 acupoints with the highest use frequency included Quchi （LI11）， Xuehai （SP10）， Zusanli （ST36）， Sanyinjiao （SP6）， Geshu （BL17） and Feishu （BL13）. Acupuncture was delivered for urticaria in terms of “syndrome differentiation and treatment” to determine the principle， and acupuncture was combined with medication as the main regimen. Among reinforcing and reducing techniques， the neutral supplementation and drainage method is the most frequently used. In the acupoint and herbal prescriptions for combined acupuncture-medication therapy of chronic urticaria： the 3 strongest acupoint pairings were Quchi （LI11） and Xuehai （SP10）， Quchi （LI11） and Zusanli （ST36）， and Xuehai （SP10） and Sanyinjiao （SP6）. The 3 strongest herb pairs were Jingjie （Schizonepeta） and Fangfeng （Saposhnikovia）， Gancao （Licorice） and Fangfeng （Saposhnikovia）， and Danggui （Angelica sinensis） and Fangfeng （Saposhnikovia）. The three strongest herb-acupoint associations were Xuehai （SP10） and Fangfeng （Saposhnikovia）， Quchi （LI11） and Fangfeng （Saposhnikovia）， and Zusanli （ST36） and Fangfeng （Saposhnikovia）.ConclusionAcupuncture is commonly combined with medication in treatment of acute and chronic urticaria. The combined therapy can solve the insufficiency presented in treatment with either simple delivery of acupuncture or Chinese herbal medicine， give a full play to the advantages of acupuncture and Chinese medicine， and advance the therapeutic effect.关键词：Acupuncture combined with medicines;Urticaria;Data mining31|11|0更新时间：2025-08-19
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