最新刊期
卷 50 ， 期 9 ， 2025
- “电针治疗哮喘小鼠，通过调控Keap1/Nrf2通路和铁死亡，减轻肺部炎症。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the activities of Kelch-like epichlorohydrin-associated protein 1 （Keap1）/nuclear factor erythroid 2-related factor 2 （Nrf2） pathway， pulmonary inflammation， and ferroptosis in house dust mite （HDM）-induced asthma model mice， so as to explore its underlying mechanisms in ameliorating asthma.MethodsFemale C57BL/6 mice were randomly allocated to 4 groups： control， model， EA， and dexamethasone （DEX） groups （n=6 per group）. The asthma mouse model was established by repeated intranasal instillation of HDM 50 μg （in PBS） on day 0， 7 and 14 （for immunization）， and 25 μg （in PBS） on day 21， 22 and 23 （for sensitization）. EA （1 mA， 2 Hz/15 Hz） was applied to “Dazhui” （GV14）， “Feishu” （BL13）， and “Zusanli” （ST36） for 30 min， once every other day for 5 times. Mice of the DEX group received intraperitoneal injection of dexamethasone （1 mg/kg） once every other day for 5 times after modeling. The mice’s general conditions were recorded and their asthma behavioral scores （0 to 9 points） were assessed according to the severity of symptoms of nasal grabbing， itch-scratching， and asthmatic attack. The mice’s pulmonary function including forced expiratory volume in 0.1 second （FEV0.1）， forced vital capacity （FVC）， the ratio of FEV0.1/FVC， and peak expiratory flow （PEF） were measured using a pulmonary function detector. Additionally， the histopathological changes， inflammation scores and airway wall thickness （total bronchial wall area ［Wat］ / bronchial basement membrane perimeter ［Pbm］） in the lung tissues were assessed using H.E. staining， periodic acid-Schiff （PAS） staining， and Masson staining， respectively. The contents of immunoglobulin E （IgE）， tumor necrosis factor-alpha （TNF-α） in serum， interleukin-1 beta （IL-1β）， and interleukin-6 （IL-6） in bronchoalveolar lavage fluid， and malondialdehyde （MDA）， glutathione （GSH）， and iron in the serum and lung homogenates were detected using ELISA and bicinchoninic acid （BCA）， separately. The protein and mRNA expression levels of Keap1， Nrf2， solute carrier family 7 member 11 （SLC7A11）， glutathione peroxidase 4 （GPX4）， acyl-CoA synthetase long-chain family member 4 （ACSL4）， and transferrin receptor 1 （TFR1） in the lung tissue were detected by Western blot and PCR， separately. The positive expressions of SLC7A11， GPX4， ACSL4， and TFR1 in the lung tissue were detected using immunohistochemical staining.ResultsCompared to the control group， the asthma behavioral score， inflammation score， Wat/Pbm， contents of TNF-α， IgE， MDA and iron in the serum， contents of IL-1β and IL-6 in the bronchoalveolar lavage fluid， and contents of MDA and iron in the lung tissue， expression levels of Keap1， ACSL4 and TFR1 proteins and mRNAs in the lung tissue， as well as the immunoactivity of pulmonary ACSL4 and TFR1 were significantly increased （P<0.01）， while the FEV0.1， ratio of FEV0.1/FVC， and the PEF， and serum and pulmonary GSH contents， expression levels of pulmonary Nrf2， SLC7A11 and GPX4 proteins and mRNAs， and immunoactivity of SLC7A11 and GPX4 significantly decreased in （P<0.01） the model group. In comparison with the model group， all the above indicators of mice in both EA and DEX groups were reversed （P<0.01， P<0.05）. The therapeutic effects of EA were strikingly superior to those of DEX in raising PEF， and serum and pulmonary GSH （P<0.05， P<0.01）， and in down-regulating serum IgE and iron， pulmonary iron， and expression levels of pulmonary ACSL4 mRNA （P<0.05， P<0.01）， but striking inferior to those of DEX in raising expression levels of pulmonary Nrf2， SLC7A11 and GPX4 proteins and mRNAs， and SLC7A11 and GPX4 immunoactivity （P<0.05， P<0.01）， and in lowering IL-6 of bronchoalveolar lavage fluid， serum and pulmonary MDA contents， and the expression of pulmonary Keap1 protein and mRNA （P<0.05， P<0.01）.ConclusionEA can alleviate HDM-induced pulmonary inflammation and decreased pulmonary function in mice with asthma， which may be related to its function in modulating ferroptosis mediated by the Keap1/Nrf2 pathway.关键词：Asthma;Electroacupuncture;Ferroptosis;Keap1/Nrf2 pathway203|60|0更新时间：2025-09-17
- “最新研究发现，“通督启神”针法通过调控NLRP3/Caspase-1/GSDMD通路，降低Aβ1-40含量，改善SAMP8小鼠认知功能。”摘要：ObjectiveTo explore the underlying mechanism of “Tongdu Qishen” acupuncture （dredging the Govern Vessel and normalizing mental activities） in treating SAMP8 mice from the perspective of pyroptosis.MethodsSix-month-old male SAMP8 mice were randomly divided into model and “Tongdu Qishen” acupuncture （EA） groups， with 12 mice in each group. SAMR1 mice of the same age （n=12） were used as the normal group. In the EA group， EA （2 Hz/50 Hz， 20 min） was applied to “Baihui” （GV20） and “Yintang” （GV24+）， and pricking bleeding was applied to “Shuigou” （GV26）. The treatment was performed once a day， for a total of 28 days. Morris water maze test was used to evaluate the learning and memory ability of mice， and HE staining was used to observe the morphology of cells in the hippocampus and cortex. Nissl staining was used to observe the number of neurons in the hippocampus and cortex. Immunohistochemistry was used to observe the expressions of Aβ1-40， interleukin （IL）-1β and IL-18 in the hippocampus and cortex. The co-staining of TUNEL and Caspase-1 was detected by immunofluorescence. Western blot and real-time quantitative PCR were used to observe the protein and mRNA expression levels of Nod-like receptor 3 （NLRP3）， Caspase-1 and Gasdermin D （GSDMD） in the hippocampus and cortex， respectively.ResultsBehavioral results showed that compared with the normal group， the escape latency was prolonged （P<0.001）， the dwell time in the original platform quadrant and the times of crossing the original platform were considerably decreased （P<0.001） in the model group. In comparison with the model group， the escape latency was shortened （P<0.05， P<0.001， P<0.01）， and the dwell time in the original platform quadrant was significantly increased （P<0.01） in the EA group. Following modeling， the number of neurons was decreased （P<0.001）， the positive expression of Aβ1-40， IL-1β and IL-18 in the hippocampus and cortex， the co-expression of TUNEL and Caspase-1， and the protein and mRNA expression levels of NLRP3， Caspase-1， GSDMD were all increased （P<0.001） in the model group relevant to the normal group. After EA intervention， modeling induced increase and decrease of indexes mentioned above were completely reversed （P<0.01，P<0.05，P<0.001）.Conclusion“Tongdu Qishen” acupuncture may improve the cognitive function of SAMP8 mice by regulating the NLRP3/Caspase-1/GSDMD pathway， relieving the release of inflammatory factors and reducing the content of Aβ1-40.关键词：Alzheimer’s disease;NLRP3/Caspase-1/GSDMD signaling pathway;Neuroinflammatory response;“Tongdu Qishen” acupuncture;Pyroptosis137|94|0更新时间：2025-09-17
- “最新研究发现，电针能改善胰岛素抵抗大鼠糖脂代谢紊乱，减轻骨骼肌脂质异位堆积，缓解炎性反应，可能与激活PI3K/Akt/GLUT4信号通路有关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on glycolipid metabolism disorders and chronic inflammatory responses in insulin-resistant （IR） rats， so as to explore the mechanism of EA in the intervention of IR.MethodsThe successfully modeled ZDF （Leprfa/fa） rats were randomly divided into the model， EA， and medication groups， with 8 rats in each group. Eight homologous control Zucker lean rats （Lepr+/fa） were taken as the control group. Rats in the EA group received EA treatment at bilateral “Sanyinjiao”（SP6） and “Zusanli”（ST36）， continuous wave applied with a frequency of 15 Hz and a current intensity of 1 mA， 20 minutes per session， once a day. Rats of the medication group were given pioglitazone solution by gavage at a dose of 10 mg/kg， while rats in the control and model groups were given the same dose of 0.9% sodium chloride solution by gavage. The above treatments were all carried out continuously for 5 days a week and rested for 2 days， for a total of 8 weeks. During the experiment， the fasting body weight （FBW） was measured weekly， and the fasting blood glucose （FBG） was detected 1 day before sample collection. After sample collection， the contents of fasting insulin （FINS）， free fatty acids （FFA）， low-density lipoprotein （LDL）， total cholesterol （TC）， triglyceride （TG）， tumor necrosis factor - α （TNF-α）， interleukin （IL）-6， IL-1β， and monocyte chemoattractant protein-1 （MCP-1） in the serum of rats were detected by ELISA， and the insulin resistance index （HOMA-IR） was calculated. The pathological changes of skeletal muscle were observed after oil red O staining. The ultrastructure of rat skeletal muscle was observed by transmission electron microscopy. The protein expression levels of phosphatidylinositol 3-kinase （PI3K）， glucose transporter 4 （GLUT4）， and the phosphorylation level of protein kinase B （Akt） in the skeletal muscle tissue of rats were detected by Western blot.ResultsCompared with the control group， the FBW， FBG， serum contents of FINS， FFA， LDL， TG， TC， IL-1β， IL-6， TNF-α， MCP-1， and HOMA-IR in the model group were increased （P<0.05，P<0.01）， the protein expressions of PI3K and GLUT4 in skeletal muscle and the phosphorylation level of Akt protein were decreased （P<0.01）. Oil red O staining showed severe lipid accumulation in skeletal muscle， and transmission electron microscopy showed a large amount of ectopic lipid accumulation in skeletal muscle tissue， swelling and deformation of mitochondria， and fragmentation of some muscle fibers. Compared with the model group， the FBW of the EA group decreased after 6 weeks of treatment， while the FBW of the medication group increased after 2 weeks of treatment （P<0.05，P<0.01）； the other indicators in the EA group and the medication group were all reversed （P<0.05，P<0.01）； oil red O staining showed that the lipid droplets in skeletal muscle cells were significantly reduced， and the degree of lipid accumulation was alleviated； transmission electron microscopy showed that the ectopic lipid accumulation in skeletal muscle tissue was reduced， and the muscle fiber fracture was alleviated.ConclusionEA can improve the glucose and lipid metabolism disorders in IR rats， reduce the ectopic lipid accumulation in skeletal muscle， and relieve the inflammatory response. This effect may be related to the activation of the PI3K/Akt/GLUT4 signaling pathway.关键词：Electroacupuncture;Insulin resistance;Diabetes;Obesity;Inflammatory response;Glucose and lipid metabolism135|65|0更新时间：2025-09-17
- “最新研究发现，电针能降低肥胖大鼠体重，提高胰岛素敏感性，可能通过激活SIRT1调节Th17/Treg平衡，抑制炎症反应。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on silent information regulator 1 （SIRT1） and the balance of Th17 and Treg in the adipose tissues of obese rats with insulin resistance， and to explore the mechanisms by which EA improves insulin resistance and obesity.MethodsMale Wistar rats were randomly divided into 5 groups： normal group， model group， EA group， sham EA group， and combination group， with 8 rats in each group. An insulin resistance obesity model was established using a high-fat diet. Rats in the EA group received EA at “Zhongwan” （CV12）， “Guanyuan” （CV4）， “Zusanli” （ST36）， and “Fenglong” （ST40）， with the needles retained for 10 min each time. The sham EA group underwent superficial needling 5 mm beside the acupoints of the EA group， with electrodes clamped but not powered， while the remaining procedures were identical to those in the EA group. The combination group was administered Sirtinol solution via tail vein injection， with the EA intervention identical to that in the EA group. All interventions were conducted 3 times a week （Monday， Wednesday， and Friday） for 8 weeks. Body mass， Lee’s index and glucose infusion rate （GIR） were measured before and after the intervention. Intraperitoneal glucose tolerance tests （IPGTT） were conducted in the 6th week of the intervention period to test the blood glucose level. After the intervention， the percentages of Th17 and Treg cells in the adipose tissue were detected by flow cytometry and the Th17/Treg ratio was calculated， while the relative expression levels of SIRT1， acetylated nuclear factor-κB （AC-NF-κB）， and interleukin-17 receptor A （IL-17A） proteins in the adipose tissue of rats in each group were analyzed by Western blot.ResultsCompared with the normal group， the model group showed increased body mass and Lee’s index （P<0.05）， elevated blood glucose level in IPGTT at all time pionts（P<0.05）， decreased GIR （P<0.05）， increased percentages of Th17 cells （P<0.05） and decreased percentages of Treg cells （P<0.05） in adipose tissue， and increased Th17/Treg ratio （P<0.05）， the expression of SIRT1 protein was decreased （P<0.05） while the expression of Ac-NF-κB and IL-17A proteins were increased （P<0.05） in adipose tissue. Compared with the model group， the EA group showed a significant decrease in body mass and Lee’s index （P<0.05）， significantly reduced blood glucose level in IPGTT at all time points （P<0.05）， increased GIR （P<0.05）， decreased percentages of Th17 cells （P<0.05） and increased percentages of Treg cells （P<0.05） in adipose tissue， and reduced Th17/Treg ratio （P<0.05）， the expression of SIRT1 protein was increased （P<0.05） while the protein expressions of Ac-NF-κB and IL-17A were decreased （P<0.05） in adipose tissue. Compared with the EA group， the combination group exhibited increases in body mass and Lee’s index （P<0.05）， elevated blood glucose level at 30， 60， and 120 min post-glucose injection in IPGTT （P<0.05）， and decreased GIR （P<0.05）， the percentage of Th17 cells was increased （P<0.05） and the Th17/Treg ratio was larger （P<0.05） in adipose tissue， and the expression of SIRT1 protein was reduced （P<0.05） while the protein expressions of Ac-NF-κB and IL-17A were increased （P<0.05） in adipose tissue.ConclusionEA can reduce body mass and improve insulin sensitivity in obese rats， and its mechanism may be related to the activation of SIRT1， which regulates the balance of Th17/Treg and inhibits inflammatory responses.关键词：Electroacupuncture;Obesity;Insulin resistance;T helper cell 17;Regulatory T cell;Balance of Th17 and Treg135|90|0更新时间：2025-09-17
- “最新研究发现，针刺“足三里”能改善佐剂性关节炎小鼠症状，可能通过促进MerTK介导的胞葬发挥抗炎作用。”摘要：ObjectiveTo observe the effect of acupuncture at “Zusanli” （ST36） on the expression of Mer receptor tyrosine kinase （MerTK）-related efferocytosis regulatory molecules in mice with adjuvant arthritis induced by complete Freund’s adjuvant （CFA）， so as to explore the potential mechanism of acupuncture on efferocytosis in adjuvant arthritis.MethodsC57BL/6J mice were randomly divided into a saline group， a model group， and an acupuncture group （10 mice/group）. The right hind footpad of mice in the saline group was injected with 0.9% sodium chloride solution， while those in the model group and the acupuncture group were injected with CFA （50 μL） to establish the model of adjuvant arthritis. Mice in the acupuncture group received acupuncture at bilateral ST36 once a day for a total of 7 times， and mice in the saline group and the model group were fixed in the same way. During the experiment， paw swelling degree and the paw withdrawal thermal latency （PWTL） of mice were measured before modeling， on the day of modeling （D0） and on the 1st， 3rd， 5th and 7th day of acupuncture （D1， D3， D5， D7） to evaluate paw swelling and pain conditions. The mRNA expressions of tumor necrosis factor （TNF）-α， interleukin （IL）-6， and IL-1β in spinal cord tissue were detected by real-time quantitative PCR. The protein expressions of MerTK， phosphorylated （p）-signal transducer and activator of transcription 3 （STAT3）， STAT3， and programmed death-ligand 1 （PD-L1） in footpad and spinal cord tissue were detected by Western blot.ResultsAfter modeling， compared with the saline group， the PWTL on D0—D7， the protein expression of PD-L1 in spinal cord and protein expressions of MerTK and PD-L1 in footpad， the phosphorylation level of STAT3 in spinal cord and footpad were decreased （P<0.01） in the model group， while the degree of paw swelling， mRNA expressions of TNF-α， IL-6， and IL-1β in spinal cord tissue were increased （P<0.01， P<0.05）. Compared with the model group， the PWTL on D1—D7， the protein expressions of MerTK， PD-L1 and phosphorylation level of STAT3 in spinal cord and footpad were increased （P<0.01） in the acupuncture group after acupuncture intervention， while the degree of paw swelling on D7， the mRNA expressions of TNF-α， IL-6， and IL-1β in spinal cord tissue were significantly decreased（P<0.01， P<0.05）.ConclusionAcupuncture can improve the symptoms of mice with adjuvant arthritis， which may be related to promoting MerTK-mediated efferocytosis and thus exerting an anti-inflammatory effect.关键词：Acupuncture;Adjuvant arthritis;Efferocytosis;Mer receptor tyrosine kinase98|76|0更新时间：2025-09-17
- 摘要：ObjectiveTo investigate the effect of moxibustion on the cyclic GMP-AMP synthase （cGAS）/stimulator of interferon genes （STING） signaling pathway in the synovial tissue of knee joints in rheumatoid arthritis （RA） model rats， and to explore the mechanism by which moxibustion alleviates synovial inflammatory injury.MethodsForty male SD rats were randomly divided into 4 groups （10 rats in each group）： blank， model， moxibustion， and triptolide groups. The RA model was induced by injecting complete Freund’s adjuvant into the right hind toe combined with exposure to wind， cold， and damp environments. The moxibustion group received daily 20 min treatment at “Zusanli” （ST36） or “Shenshu” （BL23） for 15 d； the triptolide group was administered triptolide suspension （8 mg/kg） via gavage for 15 d. The joint swelling degree of right hind toe was measured by water displacement； the synovial morphology was assessed via HE staining； the mitochondrial ultrastructure was observed by transmission electron microscopy； the synovial protein expression levels of cGAS， STING， TANK-binding kinase 1 （TBK1）， phosphorylated interferon regulatory factor 3 （p-IRF3） and phosphorylated nuclear factor-kappa B p65（p-NF-κB p65） were detected by Western blot； serum contents of interleukin-6 （IL-6）， intercellular adhesion molecule-1 （ICAM-1）， C-X-C motif chemokine ligand 10 （CXCL10）， and interferon-β （IFN-β） were measured by ELISA.ResultsCompared with the blank group， the model group showed increased joint swelling degree （P<0.01）， irregular synovial lining cell distribution， marked hyperplasia， and inflammatory cell infiltration； synovial cGAS， STING， TBK1， p-IRF3， and p-NF-κB p65 protein expression levels and serum IL-6， ICAM-1， CXCL10， and IFN-β contents were significantly elevated （P<0.01）. Compared with the model group， both moxibustion and triptolide interventions reduced joint swelling degree （P<0.01）， improved synovial histopathology， and down regulated the aforementioned proteins and cytokines （P<0.01）. No significant differences were observed between the moxibustion and triptolide groups.ConclusionMoxibustion attenuates joint swelling， inflammatory cytokine release， and synovial injury in RA model rats， potentially via suppression of the cGAS/STING pathway.关键词：Moxibustion;Rheumatoid arthritis;cGAS/STING signaling pathway;Inflammatory response133|103|0更新时间：2025-09-17
- “最新研究发现，“疏肝调神”针刺能改善缺血性脑卒中后抑郁大鼠症状，可能通过激活抗氧化系统、抑制脂质过氧化抑制铁死亡，发挥神经保护作用。”摘要：ObjectiveTo observe the effect of “Shugan Tiaoshen” needling （Smoothing the Liver and Regulating the Spirit） on antioxidant and lipid peroxidative stress in prefrontal cortex of rats with post-stroke depression （PSD）， so as to explore its mechanism in inhibiting ferroptosis of PSD neurons.MethodsSixty-four male SD rats were randomly divided into normal， sham operation， model， electroacupuncture （EA） and medication groups （12 rats in each group）. The PSD model was established by occlusion of the middle cerebral artery and reperfusion （MCAO/R）， combined with chronic unpredictable mild stimulation （CUMS）. EA was applied to “Baihui” （GV20）， “Yintang” （GV24+）， bilateral “Hegu” （LI4）， and “Taichong” （LR3） for 30 min， once daily for 14 d. Rats in the medication group were given paroxetine intragastatically （10 mg/kg） once a day for 14 d. Neurologic impairment was assessed by Zea-Longa score. Behavioral assessment of depression was conducted by sucrose preference test and open field test. The pathologic changes of prefrontal cortex were observed by HE staining. The ultrastructure and mitochondrial morphology of prefrontal neurons were observed by transmission electron microscopy （TEM）. Iron ion content in prefrontal cortex was detected by iron ion kit. The contents of glutathione （GSH） and malonicdialdehyde （MDA） in prefrontal cortex were determined by ELISA. Western blot and qPCR were used to detect the protein and mRNA expression levels of glutathione peroxidase 4 （GPX4）， solute carrier family 7 member 11 （SLC7A11）， lipoxygenase （LOX）， lysophosphatidylcholine acyltransferase 3 （LPCAT3）， and acyl-CoA synthetase long chain family member 4 （ACSL4） in prefrontal cortex.ResultsCompared with the sham operation group， the sucrose preference rate， the number of crossings in the central area， the total duration of staying in the central area， and the total distance of activity were all significantly decreased （P<0.05）， the iron ion content， MDA content， and the protein and mRNA expression levels of LOX， LPCAT3， and ACSL4 were increased （P<0.05）， while the GSH content， the protein and mRNA expression levels of GPX4 and SLC7A11 were decreased （P<0.05） in the model group. Following interventions， the above-mentioned indexes were all reversed in the EA and medication groups （P<0.05）. There were no significant differences in the above indexes between the normal group and the sham operation group， the EA group and the medication group. TEM showed unevenly distributed neurons， with an irregular triangular shape， condensed and deeply stained nuclei， and typical ferroptosis-changed mitochondria in the model group， which were relatively milder in the EA and medication groups.Conclusion“Shugan Tiaoshen” needling can improve the depressive symptoms of ischemic PSD rats， and its mechanism may be related to activating the prefrontal antioxidant system， inhibiting lipid peroxidative and iron death， thus playing a protective role in neurons.关键词：Post stroke depression;Acupuncture;Ferroptosis;Lipid peroxidative137|92|0更新时间：2025-09-17
- “最新研究发现，“化瘀通络”灸能改善血管性痴呆大鼠学习记忆能力，促进髓鞘再生，优化神经传导功能。”摘要：ObjectiveTo observe the effect of “Huayu Tongluo”（blood stasis-removing and meridian-collateral dredging） moxibustion on oligodendrocyte-related protein expression and myelin ultrastructure in the corpus callosum tissue of rats with vascular dementia （VD）， so as to explore its mechanism underlying improvement of VD.MethodsMale SD rats were randomly divided into model group， “Huayu Tongluo” moxibustion group and non-acupoint moxibustion group， with 42 rats in each group. According to the treatment time， the rats were further divided into 1 course （7 d） group （n=12）， 2 courses （14 d） group （n=12） and 4 courses （28 d） group （n=18），and the other 18 rats were used as the sham operation group. The VD model was established by using modified 2-vessel occlusion （VO） method. The rats in the “Huayu Tongluo” moxibustion group were treated with suspended moxibustion of “Baihui”（GV20）， “Dazhui”（GV14） and “Shenting”（GV24） for 20 min， once a day for 7 d， 14 d or 28 d， respectively. The rats of the non-acupoint moxibustion group were treated with suspended moxibustion at the three locations 2， 3 and 5 cm away from the tail， and the course of treatment was the same as that of “Huayu Tongluo” moxibustion group. Morris water maze test was used to assess the rats’ learning-memory ability. A transmission electron microscopy was used to observe the ultrastructure of myelin sheath and the number of myelinated nerve axons， and to calculate the g-ratio （axon diameter/（axon diameter + myelin sheath thickness）） of the myelin sheath thickness. Western blot and immunofluorescence staining were used to separately detect the expressions and fluorescence intensities of oligodendrocyte markers 2’，3’-cyclic nucleotide-3’-phosphodiesterase （CNPase）， oligodendrocyte transcription factor 2 （Olig2） and myelin oligodendrocyte glycoprotein （MOG） in the corpus callosum of each group.ResultsCompared with the sham operation group， the model group showed prolonged escape latency （P<0.01） and reduced number of platform quadrant crossings （P<0.01） in the 1， 2 and 4 courses of treatment， significantly decreased number of myelinated nerve axons in the corpus callosum （P<0.01）， increased g-ratio value of axonal myelin （P<0.01）， and decreased relative fluorescence intensities and expression levels of CNPase， Olig2， and MOG proteins in the 1， 2 and 4 courses of intervention （P<0.01）. Compared with the model group， the “Huayu Tongluo” moxibustion group showed shortened escape latency （P<0.01） and increased number of platform quadrant crossing after 2 and 4 courses of intervention （P<0.01）， significantly increased number of myelinated nerve axons （P<0.01）， decreased g-ratio value of axonal myelin， and significantly elevated relative fluorescence intensities and protein expression levels of CNPase， Olig2， and MOG in all the different courses of intervention （P<0.01，P<0.05）； and the non-acupoint moxibustion group had an increase in the immunofluorescence intensities of CNPase and MOG， and the expression of CNPase protein in the 4 courses of intervention （P<0.05）. Comparison between the two treatment groups showed that the effect of “Huayu Tongluo” moxibustion was significantly superior to that of non-acupoint moxibustion in lowering the g-ratio of axonal myelin （P<0.05）， and in up-regulating the number of myelinated nerve axons， and the immunofluorescence intensities and protein expressions of CNPase， Olig2 and MOG in the 3 different courses of intervention （P<0.01， P<0.05）.Conclusion“Huayu Tongluo” moxibustion can improve the learning-memory ability of VD rats， which may be related to its functions in promoting the proliferation and differentiation of oligodendrocyte precursor cells into mature oligodendrocytes， and up-regulating the protein expressions of CNPase， Olig2 and MOG in the corpus callosum. The therapeutic effect was the best in the 4 courses of intervention.关键词：Vascular dementia;“Huayu Tongluo”moxibustion;Oligodendrocytes;Myelin regeneration;Corpus callosum97|59|0更新时间：2025-09-17
- “最新研究发现，电针能改善慢性应激引起的小鼠焦虑行为，通过调节大脑前额叶皮层和海马区神经元兴奋抑制平衡发挥作用。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the excitatory/inhibitory （E/I） balance of prefrontal cortex and hippocampal pyramidal neurons in anxiety-like mice induced by chronic unpredictable mild stress （CUMS）.MethodsForty-eight male C57BL/6 mice were randomly divided into control， control+EA （CE）， model， and model+EA （ME） groups， with 12 mice in each group. The anxiety model was established by using CUMS （restraint， day-night reversal， forced swimming in warm water， food/water deprivation for 24 h， rearing in a humid environment， and shaking the cage） for 21 consecutive days. EA （2 Hz， 1 mA） was applied to “Baihui” （GV20） and “Yintang” （GV24+） for 30 min， once daily for 10 consecutive days. The anxiety-like behaviors of mice were evaluated by open field test and elevated plus maze （EPM） test. The E/I balance of the prefrontal cortex and hippocampal pyramidal neurons of the mice were observed by whole-cell recording.ResultsAfter modeling， the exploration time in the central area of open field test， the times of entries and residence time in the open arm of EPM test were significantly reduced （P<0.01） in the model group， while the spontaneous excitatory postsynaptic current （sEPSC） frequency， capacitance and E/I ratio of sEPSC in the dorsolateral prefrontal cortex （dlPFC）， medial prefrontal cortex （mPFC） and ventral hippocampal CA1 （vCA1） were increased significantly （P<0.01）. Compared with the model group， the exploration time in the central area of the open field， the times of entries and residence time in the open arm of EPM test were significantly increased （P<0.05， P<0.01）， while the frequency， capacitance and E/I ratio of sEPSC in the dlPFC， mPFC and vCA1 were significantly decreased （P<0.01， P<0.05） in the ME group. In comparison with the CE group， the exploration time in the central area of the open field， the times of entries and residence time in the open arm of EPM test were significantly reduced （P<0.01）， while the frequency， capacitance and E/I ratio of sEPSC in the dlPFC， mPFC and vCA1 were significantly increased （P<0.01， P<0.05） in the ME group.ConclusionChronic stress may induce anxiety in mice by increasing the E/I of pyramidal neurons in dlPFC， mPFC and vCA1. EA may improve anxiety-like behaviors in mice by regulating the E/I balance of the above brain regions.关键词：Chronic unpredictable mild stress;Anxiety disorder;Electroacupuncture;Excitatory/inhibitory balance85|52|0更新时间：2025-09-17
- “最新研究发现，电针特定穴位能缓解慢性偏头痛，降低痛觉敏化，为偏头痛治疗提供新途径。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） at specific points of the Shaoyang Meridian on mechanical pain threshold， and expressions of P2Y12 receptor （P2RY12）， ionic calcium binding molecule 1 （Iba-1）， calcitonin gene-related peptide （CGRP） and c-fos in rats with chronic migraine （CM） ， so as to explore its mechanisms underlying prevention and treatment of CM.MethodsForty-eight SD rats （half male and half female） were randomly divided into 6 groups （using a random number table method）： control， model， EA， antagonist， EA+agonist， and medication groups， with 8 rats in each group. The CM model was established by repeated intraperitoneal injection of nitroglycerin （10 mg/kg， once every other day， 5 times altogether）. EA （10 Hz/50 Hz， a strength being able to induce local slight regular muscular contraction of the fore-limb） was applied to bilateral “Fengchi”（GB20）， “Yanglingquan”（GB34）， and “Waiguan”（TE5） for 30 min， once a day before modeling and once every other day during modeling， for a total of 6 times. The rats of the antagonist group received intrathecal injection of P2RY12 antagonist MRS2395 （200 μg/rat） ， once every other day for a total of 5 times. Rats of the EA+agonist group received intrathecal injection of P2RY12 agonist ADP （4.272 μg/rat） once every other day for a total of 5 times， and those of the medication group received intraperitoneal injection of topiramate （30 mg/kg） once a day for 9 d. Rats of the other groups were bound for 30 min during EA. On the 1st， 3rd， 5th， 7th， and 9th day of modeling， 2 h before the intervention， the mechanical pain thresholds of the periorbital region of the eye and the plantar region of the hindlimb in each group were measured using a von Frey filament. The number of P2RY12 positive cells and the expression of P2RY12 mRNA in the caudal nucleus of the trigeminal spinal tract （TNC） were detected using immunofluorescence staining and real-time fluorescence quantitative PCR， separately， and the expression levels of P2RY12， Iba-1， CGRP， and c-fos proteins in the TNC tissue were detected using Western blot.ResultsIn comparison with the control group， the periorbital and plantar pain thresholds were significantly reduced （P<0.001）， while the expression levels of P2RY12， Iba-1， CGRP， and c-fos proteins in the TNC tissue， and the number of P2RY12 positive cells and P2RY12 mRNA expression were significantly increased in the model group （P<0.001）. Compared with the model group， the periorbital and plantar pain thresholds of the EA， antagonist and medication groups were significantly increased （P<0.001）， while the expression levels of P2RY12， Iba-1， CGRP， and c-fos proteins， number of P2RY12 positive neurons， and P2RY12 mRNA expression were apparently decreased in the EA， antagonist， medication and EA+agonist groups （P<0.001， P<0.05）. The effect of EA was strikingly superior to that of EA+agonist in increasing periorbital and plantar pain thresholds （P<0.001， P<0.05）， lowering the expressions of P2RY12， Iba-1， CGRP and c-fos， the number of P2RY12 positive cells， and P2RY12 mRNA expression （P<0.05， P<0.001）， and superior to that of medication in lowering the number of P2RY12 positive cells and P2RY12 mRNA expression （P<0.05， P<0.01）.ConclusionEA of the specific points of Shaoyang Meridian can prevent the progression of migraine， and reduce pain， which may be related to its function in down-regulating the expressions of P2RY12 protein and mRNA， Iba-1， and CGRP and c-fos proteins （central sensitization-related indexes） in TNC.关键词：Chronic migraine;Electroacupuncture;P2Y12 receptor;Specific acupoints of Shaoyang meridian77|52|0更新时间：2025-09-17
- “最新研究发现，刺络拔罐法能有效降低内毒素致热家兔体温，其机制可能涉及改善下丘脑线粒体功能和调节ATP合成相关蛋白。”摘要：ObjectiveTo observe the effect of blood-letting plus cupping on endotoxin-induced fever in rabbits and to explore its central antipyretic mechanism based on mitochondrial energy metabolism.MethodsSeventy-two male Japanese white rabbits were randomly divided into six groups： blank control， model， medication， blood-letting， cupping， and blood-letting cupping groups， with 12 rabbits in each group. The fever model was established by injection of endotoxin （200 ng/kg） into the ear vein. One hour after modeling， the rabbits in the medication group received gavage of indomethacin， while the other groups were given the same volume of physiological saline via gavage. The rabbits in the blood-letting group received blood-letting at the “Dazhui” （GV14） acupoint， those of the cupping group received cupping at the GV14 area with -0.02 MPa pressure for 15 min， and those of the blood-letting cupping group first underwent blood-letting at GV14 followed by cupping with the same parameters as those of the simple blood-letting and simple cupping groups. Temperature changes in each group were recorded at 1， 2， 3， 4， 5 and 6 h after modeling. A transmission electron microscopy was used to observe the ultrastructure of hypothalamic mitochondria， the ELISA was employed to measure the contents of serum IL-1β and IL-6. An Oxygraph-2k was used to assess the mitochondrial respiratory function， the ATP content was determined with an ATP assay kit， and the expression levels of uncoupling protein 2 （UCP2） and mitofusin 2 （Mfn2） in the hypothalamic tissue were measured using the WES （automatic Western blot quantitative analysis system）.ResultsCompared with the blank control group， the model group showed a significant increase in the body temperature from 1 h on after modeling （P<0.01）， contents of serum IL-1β and IL-6 （P<0.01）， and the expression of hypothalamic UCP2 protein （P<0.01）， and a significant decrease in the mitochondrial oxygen consumption （P<0.05） and ATP content （P<0.01）， and the expression of Mfn2 protein in the hypothalamic tissue （P<0.01）. Compared to the corresponding time points of the model group， the body temperature at 2， 3， 4， 5 and 6 h in both medication and blood-letting cupping groups， at 2， 3， 4 and 6 h in both cupping and blood-letting groups were significantly decreased （P<0.01， P<0.05）. No significant differences were found between the medication and blood-letting cupping groups， and between the cupping and blood-letting groups in lowering the body temperature at the five time points. The effects of simple cupping at 2， 3， 5 and 6 h and simple blood-letting at 2， 3， 4， 5 and 6 h were respectively obviously weaker than those of the medication in lowering body temperature at the corresponding time points （P<0.01， P<0.05）. The effects of simple cupping at 5 and 6 h and simple blood-letting at 2， 3， 5 and 6 h were obviously weaker in lowering body temperature than those of the blood-letting cupping at the corresponding time points （P<0.01， P<0.05）. Compared with the model group， the serum IL-1β and IL-6 contents and UCP2 expression level in hypothalamic tissue were strikingly down-regulated in the 4 intervention groups （P<0.05， P<0.01）， except IL-6 in the blood-letting group， while the mitochondrial oxygen consumption， serum ATP content and Mfn2 expression level in hypothalamic tissue were considerably up-regulated in the 4 intervention groups （P<0.05， P<0.01）， except ATP content in the blood-letting group. No significant differences were found among the 4 intervention groups in the levels of serum IL-1β and IL-6， UCP2 and Mfn2 expressions in hypothalamic tissue， oxygen consumption and ATP content. Results of the hypothalamic mitochondrial ultrastructure showed severe damage， including blurred and broken cristae or even disappearance of the cristae in the model group， which was milder in the injury severity in the 4 intervention groups， including incomplete structure of the bilayer membrane， but without obvious fracture.ConclusionBlood-letting cupping intervention has a significant antipyretic effect in rabbits with endotoxin-induced fever， which may be related to its functions in improving mitochondrial respiratory function and regulating the expression of key proteins （UCP2 and Mfn2） involving ATP synthesis in the hypothalamus.关键词：Fever;Blood-letting and cupping;Hypothalamus;Mitochondria;Energy metabolism85|56|0更新时间：2025-09-17
- “最新研究发现，耳穴电刺激能有效缓解小鼠瘙痒症状，作用机制可能与抑制H1R/TRPV1信号通路相关。”摘要：ObjectiveTo observe the effect of auricular electrostimulation on pruritus behavior， histomorphology and expressions of histamine 1 receptor （H1R） and transient receptor potential vanilloid subfamily 1 （TRPV1） of the scratching site， serum immunoglobulin E （IgE） and histamine （HIS） contents of pruritic mice， so as to explore its underlying mechanisms in improvement of pruritus.MethodsTwenty-four Kunming mice were randomly divided into control， model， medication and auricular electrostimulation groups， with 6 mice in each group. The mice in the control and model groups received gavage of 0.2 mL/20 g of 0.9% sodium chloride solution， once every day for 12 d. The mice in the medication group received gavage of loratadine saline solution （1.66 mg/kg）. The mice in the auricular electrostimulation group received auricular stimulation of bilateral “Xin”（Heart）-“Fei” （Lung） or margin of ear for 30 min， once daily for 12 d. In addition， all the mice in every group received grabbing-binding once every day for 12 d. One hour after the last intervention， the pruritus model was established by intravenous injection of 0.025% dextran （0.05 mL/10 g） via tail vein. The scratching response of mice was recorded， and the histopathological changes of the scratching site were observed after H.E. staining. The immunofluorescence intensities of H1R and TRPV1 were detected by immunofluorescence staining， and the contents of serum IgE and HIS were measured by using ELISA.ResultsCompared with the control group， the model group had a decrease in the scratching latency， and an increase in the number and duration of scratching， the immunofluorescence intensities of H1R and TRPV1， and the contents of serum IgE and HIS （P<0.05）. In contrast to the model group， both the medication group and auricular electrostimulation group had an increase in the scratching latency， and a decrease in the number and duration of scratching， the immunofluorescence intensities of H1R and TRPV1， and the contents of serum IgE and HIS （P<0.05）. There were no significant differences between the auricular electrostimulation group and medication group in all the indexes mentioned above. H.E. staining showed cell edema in the skin epidermal tissue， a large number of lymphocytes and a few eosinophils infiltration in the dermis and subcutaneous tissue， especially around the hair follicle in the model group， while the inflammatory cells in the skin tissue of the medication group and the auricular electrical stimulation group were reduced.ConclusionAuricular electrostimulation can effectively relieve pruritus induced by dextran in mice， which may be related to its function in down-regulating the H1R/TRPV1 signaling pathway in the pruritus site.关键词：Pruritus;Auricular electrostimulation;Histamine 1 receptor;Transient receptor potential vanilloid subfamily 172|73|0更新时间：2025-09-17
- “最新研究发现，调督理筋针法联合理筋整复手法治疗神经根型颈椎病效果显著，能减轻疼痛，降低炎性指标，改善椎-基底动脉血流与颈椎功能。”摘要：ObjectiveTo explore the therapeutic effect of the combined measures of Tiaodu Lijin acupuncture therapy （for regulating the governor vessel and tendons） and Lijin reduction tuina manipulation （for regulating tendons and reduction） in treatment of cervical spondylotic radiculopathy （CSR）.MethodsFrom August 2021 to December 2023， 160 CSR patients were selected and randomly divided into a control group （80 cases） and a trial group （80 cases） using a random number table method. In the control group， the conventional acupuncture and Lijin reduction tuina manipulation were operated， and in the trial group， Tiaodu Lijin acupuncture therapy and Lijin reduction tuina manipulation were combined. The treatment was delivered once daily in each group， 5 interventions a week and lasted for 4 weeks. Before and after treatment， the score of clinical assessment scale of cervical spondylosis （CASCS）， cervical dysfunction index （NDI） and the score of visual analog scale （VAS） were compared in the two groups. Using ELISA， the contents of interleukin （IL）-6， leukotriene D4 （LTD4）， tumor necrosis factor （TNF）-α and C-reactive protein （CRP） in the serum were detected. Transcranial color Doppler ultrasound was used to detect the mean peak velocity （VM）， resistance index （RI） and pulsation index （PI） of vertebral-basilar artery at systole. The effective rate was calculated in the two groups.ResultsEvery indicator after treatment was improved in comparison with that before treatment in each group （P<0.05）. After 4-week treatment， compared with the control group， NDI and VAS scores were lower （P<0.05）， and the serum contents of IL-6， LTD4， TNF-α and CRP were reduced （P<0.05）， RI and PI of vertebral-basilar artery were lower （P<0.05）， and CASCS score and VM were higher （P<0.05） in the trial group. The overall effective rate in the trial group （95.00%， 76/80） was higher （P<0.05） than that （81.25%， 65/80） in the control group.ConclusionThe combined measures of Tiaodu Lijin acupuncture therapy and Lijin reduction tuina manipulation obtain the satisfactory therapeutic effect on CSR， which alleviates pain， reduces inflammatory indicators and improves vertebral basilar arterial blood flow and cervical spine function.关键词：Cervical spondylotic radiculopathy;Tiaodu Lijin acupuncture therapy;Lijin reduction tuina manipulation152|85|0更新时间：2025-09-17
- “最新研究发现，针刺通过调节GABA能系统改善痛情绪，为临床治疗提供新思路。”摘要：Pain-related emotions refer to the negative emotions such as depression and anxiety triggered by pain， which seriously impact patients’ physical and mental health as well as the quality of life. The gamma-aminobutyric acid （GABA） ergic system is the primary inhibitory neurotransmitter system in the brain and plays a significant role in the onset and development of pain-related emotions. Acupuncture can effectively ameliorate the negative emotions such as depression and anxiety caused by pain. Its mechanism is closely related to the regulatory effect of acupuncture on GABAergic system. Acupuncture is active in modulating GABAergic neurons， up-regulating the expression of GABAergic neuron subtype parvalbumin neurons and down-regulating the expression of GABAergic neuron subtype neuropeptide Y； up-regulating the mRNA expression of GABAA receptor （GABAAR） and the protein expression of GABAB2 in the amygdala， and down-regulating the protein expression of GABAAR in the anteiror cingulate cortex. Besides， acupuncture can regulate the expressions of the key factors such as gutamate decarboxylase and gama-amino-butyrate transporter that affect the GABAergic system， thereby relieve pain-related emotions. These findings provide the references for the mechanism research and clinical application of acupuncture in treatment of pain-related emotions.关键词：Acupuncture;Pain-related emotions;GABAergic system;Review158|100|0更新时间：2025-09-17
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