最新刊期
卷 51 ， 期 1 ， 2026
- “最新研究发现，电针治疗能显著促进大鼠多裂肌损伤修复，可能与穴区外泌体释放有关。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the expressions of paired box transcription factor 7 （Pax7）， myogenic differentiation antigen （MyoD）， myogenin （MyoG） and myosin heavy chain （MyHC） in the multifidus muscle， and CD63， programmed cell death protein 6 interacting protein （Alix） and tumor susceptibility gene 101 （TSG101） proteins in the serum exosomes in rats with lumbar multifidus muscle injury （MFMI）， so as to explore the effect of exosomes in acupoint areas on EA improvement of muscular regeneration and repair.MethodsForty male SD rats were randomly divided into normal control， model， EA and EA+exosome inhibitor （EA+inhibitor） groups， with 10 rats in each group. The MFMI model was established by injection of 0.5% bupivacaine （150 μL × 4） into the 4 points of the multifidus muscle along the bilateral lumbar （L）4—L5 spinous processes. EA （2 Hz/10 Hz， 1 mA） was applied to bilateral “Weizhong” （BL40） and “Shenshu” （BL23） for 20 min， once a day for 7 d. For rats of the EA+inhibitor group， exosome inhibitor GW4869 （3 mg/mL， 50 μL/acupoint） was injected into bilateral BL40 and BL23 1 h before each EA intervention. The morphological changes of the multifidus muscle were observed after H.E. staining and Masson staining. The immunoactivity of Pax7 and MyoD was observed by immunohistochemistry. The serum exosomes were extracted and identified by transmission electron microscope （TEM） and nanoparticle tracking analysis （NTA）. The expression levels of MyoG and MyHC in the multifidus muscle tissue and CD63， Alix and TSG101 proteins in the serum exosomes were detected by Western blot.ResultsMorphological results showed that in the model group， most of the muscle fibers were degenerated and necrotic， a large number of inflammatory cells infiltrated around the muscle fibers and more blue-stained collagen fibers were observed. In the EA group， the morphology of muscle fibers was relatively complete， with more new muscle fibers and reduced inflammatory cells in the injured area， and the collagen fibers were significantly reduced. In the EA+inhibitor group， there were still more muscle fiber destruction and inflammatory cell infiltration， new muscle fibers with uneven diameter and more collagen fibers. Compared with the normal control group， the immunoactivity of Pax7 in the multifidus muscle， the expression of Alix and CD63 proteins in the serum exosomes were significantly increased in the model group （P<0.01， P<0.05， P<0.001）. In comparison with the model group， the immunoactivity of Pax7 and MyoD， the expression levels of Alix and TSG101 in the serum exosomes and MyHC and MyoG proteins in the multifidus muscle were considerably up-regulated in the EA group （P<0.01， P<0.05）. After local injection of GW4869 at BL40 and BL23， the immunoactivity of Pax7 and MyoD， the protein expression levels of TSG101， CD63， MyHC and MyoG were significantly lower in the EA+inhibitor group than those of the EA group （P<0.01， P<0.05， P<0.001）. The results of TEM and NTA showed that the exosomes were successfully extracted. The morphology of the exosomes was typical saucer-like under electron microscope， and the particle size range was concentrated in 70—200 nm.ConclusionEA of BL40 and BL23 can significantly up-regulate the expressions of Pax7， MyoD， MyoG and MyHC in the injured multifidus muscle， and promote the regeneration and repair of lumbar multifidus muscle， which may be related to its functions in promoting the release of exosomes in the acupoint area.关键词：Electroacupuncture;Multifidus muscle injury;Acupoints;Serum exosomes;Muscle repair and regeneration68|45|0更新时间：2026-01-21
- “最新研究发现，电针治疗能改善大脑中动脉缺血再灌注引起的大鼠学习记忆障碍，通过调节线粒体分裂融合发挥神经保护作用。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on mitochondrial fission/fusion in rats with learning-memory impairment induced by cerebral ischemia-reperfusion （CI/R）， so as to explore its potential neuroprotective mechanisms against the ischemic reperfusion injury of hippocampal neurons.MethodsMale SD rats were randomly divided into normal， sham-operation， model， and EA groups， with 12 rats in each group. The CI/R model was established by middle cerebral artery occlusion and reperfusion by using the intraluminal suture method. After successful modeling， rats in the EA group received EA （2 Hz/10 Hz， 6 V） at “Shenting”（GV24） and “Baihui” （GV20） for 30 min， once daily for 14 consecutive days. The rats’ learning-memory ability was evaluated using the novel object recognition test and Morris water maze test. The cerebral infarct volume was assessed by 2，3，5-triphenyltetrazolium chloride （TTC） staining. Hippocampal neuronal morphology was examined by HE staining， and the mitochondrial morphology and structure of hippocampal neurons were observed using transmission electron microscopy. The adenosine triphosphate （ATP） content in the hippocampal tissue was measured using colorimetry， and mitochondrial DNA （mtDNA） copy number was detected by qPCR. The relative protein and mRNA expression levels of mitochondrial fusion proteins 1/2 （MFN1/2）， optic atrophy protein 1 （OPA1）， dynamin-related protein 1 （DRP1）， and fission protein 1 （FIS1） in the hippocampal tissue were detected by Western blot and qPCR respectively.ResultsNo significant differences were found between the normal and sham-operation groups in all the indices. Compared with the sham-operation group， the model group exhibited a significant increase in the escape latency of Morris water maze test from the 3rd to the 5th day， brain infarction volume， expression levels of DRP1 and FIS1 protein and mRNA （P<0.01， P<0.05）， and a significant decrease in the novel object recognition index， crossing number of the original target platform in Morris water maze test， copy number of mtDNA， ATP content， and the expression levels of MFN1， MFN2， and OPA1 protein and mRNA （P<0.01， P<0.05）. In comparison with the model group， both the increased levels of the escape latency， brain infarction volume， expressions of DRP1 and FIS1 protein and mRNA， and the decreased levels of the novel object recognition index， crossing number of the original target platform， copy number of mtDNA， ATP content， and the expressions of MFN1， MFN2， and OPA1 protein and mRNA were reversed in the EA group （P<0.01， P<0.05）. Histological examination showed that in the model group， the hippocampal neurons were loosely arranged， with extensive vacuolar degeneration and necrosis， swollen and ruptured mitochondria， irregular mitochondrial membrane， and disappearance of cristae， while in the EA group， hippocampal neurons were closely arranged and relative intact in the morphology， with relatively complete mitochondria， and clear cristae.ConclusionEA can improve the learning-memory ability in CI/R rats which may be related to its functions in inhibiting the mitochondrial fission and promoting mitochondrial fusion， thus maintaining mitochondrial function and structural stability.关键词：Electroacupuncture;Cerebral ischemia/reperfusion;Hippocampal neuron;Mitochondrial fission/fusion;Learning-memory ability43|54|0更新时间：2026-01-21
- “最新研究发现，电针“神庭”“百会”能改善脑缺血再灌注大鼠学习记忆功能，可能通过修复血脑屏障、降低促炎因子水平，抑制小胶质细胞活化及中枢神经炎性反应。”摘要：ObjectiveTo observe the effects of electroacupuncture （EA） on learning memory and central inflammatory response in rats with middle cerebral artery occlusion/reperfusion （MCAO/R）， and to explore its potential mechanisms.MethodsA total of 88 SD rats were randomly divided into a blank group （14 rats）， a sham operation group （14 rats）， and the remaining 60 rats were used to establish the MCAO/R model using the suture-occlusion method. The successfully modeled rats were randomly divided into a model group and an EA group， with 14 rats in each group. Rats of the EA group received EA at “Shenting” （GV24） and “Baihui” （GV20） for 30 min， once daily for 14 consecutive days. After intervention， neurological function and learning memory were evaluated using the Zea-Longa score and Morris water maze test. The brain infarction volume was assessed using TTC staining. Neuronal damage was observed using Nissl staining. Immunofluorescence was used to detect the positive expression of ionized calcium-binding adapter molecule 1 （Iba1） in the hippocampal CA1 region. ELISA was used to measure the levels of pro-inflammatory cytokines interleukin （IL）-6， IL-1β， tumor necrosis factor （TNF）-α， and the blood-brain barrier-related factor S100β in serum. Western blot was used to detect the relative expression levels of Iba1， inflammatory factors， and tight junction proteins zonula occludens-1 （ZO-1）， Occludin， and Claudin5 in the hippocampal tissue.ResultsCompared with the sham operation group， rats of the model group showed increased neurological function scores （P<0.01）， prolonged escape latency （P<0.01）， reduced times of crossings over the original platform （P<0.01）， increased brain infarction volume （P<0.001）， abnormal hippocampal neuronal morphology， increased number of Iba1-labeled microglia （P<0.01）， elevated relative expression level of Iba1 protein （P<0.01）， increased protein expression levels of TLR4， NF-κB， IL-6， IL-1β and TNF-α in the hippocampal tissue （P<0.01）， decreased protein expression levels of ZO-1， Occludin and Claudin5 （P<0.01）， and increased contents of IL-6， IL-1β， TNF-α and S100β in serum （P<0.01）. After EA intervention， compared with those of the model group， rats of the EA group showed decreased neurological function scores （P<0.01）， shortened escape latency （P<0.01）， increased times of crossings over the original platform （P<0.01）， reduced brain infarction volume （P<0.01）， more regular neuronal morphology， decreased number of Iba1-labeled microglia （P<0.01）， decreased protein expression levels of TLR4， NF-κB， Iba1， IL-6， IL-1β， and TNF-α in the hippocampal tissue （P<0.01， P<0.05）， increased protein expression levels of ZO-1， Occludin， and Claudin5 （P<0.01， P<0.05）， and decreased contents of IL-6， IL-1β， TNF-α and S100β in serum （P<0.01）.ConclusionEA at GV24 and GV20 can improve learning memory function in MCAO/R rats， and its mechanism may be related to the repair of the central blood-brain barrier， reduction of pro-inflammatory cytokine levels， and inhibition of microglial activation and central neuroinflammation.关键词：Electroacupuncture;Cerebral ischemia-reperfusion;Learning memory impairment;Microglia;Central inflammatory response12|6|0更新时间：2026-01-21
- “在缺血性中风治疗领域，研究者通过实验验证了朱琏抑制Ⅱ型针法对大鼠神经功能和血管生成的积极影响，为中风治疗提供新方案。”摘要：ObjectiveTo observe the effects of Zhu Lian’s type Ⅱ inhibition needling on neurological function and angiogenesis in rats with ischemic stroke （IS）， so as to explore its potential mechanisms.MethodsWistar rats were divided into sham operation group， model group， acupuncture group， and acupuncture+angiogenin （Ang）-2 group， with 12 rats in each group. A suture method was used to establish the IS model. The acupuncture group received Zhu Lian’s type Ⅱ inhibition needling at the “Guanyuan” （CV4）， “Guilai” （ST29）， “Zusanli” （ST36）， “Sanyinjiao” （SP6）， and “Fenglong” （ST40） acupoints for 30 min per session. Rats in the acupuncture+Ang-2 group were injected via the tail vein with Ang-2 recombinant protein （10 mg/kg） before Zhu Lian’s type Ⅱ inhibition needling intervention. All treatments were administered once daily for 7 consecutive days. Neurological deficit scores were assessed in rats of each group. TTC staining was employed to determine the percentage of cerebral infarction volume. HE staining was used to observe the pathological changes in the ischemic penumbra cortex. Immunohistochemistry was used to evaluate microvascular density in the ischemic penumbra cortex. Western blot was conducted to detect the expression levels of vascular endothelial growth factor （VEGF）， vascular endothelial growth factor receptor 2 （VEGFR-2）， Ang-1， Ang-2， tyrosine kinase 2 （Tie-2）， and phosphorylated Tie-2 （p-Tie-2） proteins in the ischemic penumbra cortex.ResultsCompared with the sham operation group， the model group showed elevated neurological deficit scores， cerebral infarction volume percentage， microvascular density in the ischemic penumbra cortex， and protein levels of VEGF， VEGFR-2， Ang-1， and p-Tie-2/Tie-2 ratio. Conversely， Ang-2 protein level was decreased （P<0.05）. Compared with the model group and the acupuncture+Ang-2 group， rats in the acupuncture group had lower neurological deficit scores， cerebral infarction volume percentage， and Ang-2 protein level. While microvascular density in the ischemic penumbra cortex and the protein levels of VEGF， VEGFR-2， Ang-1， and the p-Tie-2/Tie-2 ratio were further increased （P<0.05）. In the model group， the number of neurons in the ischemic penumbra cortex of rats was decreased， the nuclei were shrank or disappeared， the cell bodies were vacuolated and degenerated， accompanied by a large number of inflammatory cell infiltrations. Compared with the model group， the injury of cortical neurons in the acupuncture group was alleviated. Compared with the acupuncture group， the injury of cortical neurons in the acupuncture+Ang-2 group was aggravated.ConclusionZhu Lian’s type Ⅱ inhibition needling can improve neurological function in IS rats， possibly by activating the Ang-1/Tie-2 pathway to promote angiogenesis.关键词：Ischemic stroke;Zhu Lian’s type Ⅱ inhibition needling;Angiogenesis;Angiopoietin-1/tyrosine kinase 2 signaling pathway28|39|0更新时间：2026-01-21
- “最新研究发现，电针“内关”“神门”能改善慢性心力衰竭大鼠心功能，可能通过调控Notch信号通路实现。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on the activities of N-terminal B-type natriuretic peptide （NT-proBNP）/vascular endothelial growth factor （VEGF）/neurogenic site-gap homologous protein （Notch） signaling pathway and related factors in rats with chronic heart failure （CHF）， so as to explore its regulatory mechanism underlying improvement of CHF.MethodsMale SD rats were randomly divided into sham-operation， CHF model， EA and EA+inhibitor ［DAPT （GSI-IX）， a γ-secretase inhibitor］ groups （n=6 in each group）. The CHF model was established by ligating the anterior descending branch of the left coronary artery. EA （2 Hz， an electric current intensity to induce slight shaking of the inserted acupuncture needles） was applied to bilateral “Neiguan”（PC6） and “Shenmen”（HT7） for 30 min， once daily， for 7 d. Rats of the EA+inhibitor group received intraperitoneal injection of DAPT （3 mg/kg） 30 min before each EA intervention. A small animal color Doppler ultrasound machine was used to collect the left ventricular ejection fraction （LVEF）， left ventricular fractional shortening rate （LVFS）， left ventricular end-systolic diameter （LVIDs） for evaluating the left ventricle function. Histopathological changes of the cardiac tissue were observed by H.E. staining and Masson staining. The contents of serum NT-proBNP and VEGF were detected by using ELISA. The microvascular density （MVD） of the left ventricular myocardium was observed by CD34 immunohistochemistry. The expression levels of myocardial Notch signaling pathway-related proteins Notch1 and Delta-like 4 ligand （DLL4） were detected by using Western blot.ResultsCompared with the sham operation group， the model group had a significant increase in the LVIDs， serum NF-proBNP content， MVD and expression levels of Notch1 and DLL4 protein in the left ventricular myocardial tissue （P<0.000 1， P<0.001， P<0.01）， and a significant decrease in the LVEF， LVFS and serum VEGF content （P<0.000 1）. In comparison with the model group， the EA group had a significant decrease in the LVIDs and serum NF-proBNP content（P<0.001）， and a significant increase in the LVEF， LVFS and serum VEGF content （P<0.001， P<0.000 1）， while the MVD and expression levels of Notch1 and DLL4 protein in the left ventricular myocardial tissue were more increased （P<0.001， P<0.05， P<0.01）. In comparison with the EA group， both the striking increase and decrease of the indexes mentioned above were all reversed in the EA+inhibitor group （P<0.05， P<0.01， P<0.000 1， P<0.001） except LVIDs. Histopathological observation showed obvious myocardial fiber rupture， irregular arrangement， cell necrosis， unclear nucleoli， fibrous hyperplasia， deposition of collagenous fibers， and a large number of inflammatory infiltration in the myocardial tissue of the model group， which was relatively milder in the EA group but not in the EA+inhibitor group.ConclusionEA of PC6 and HT7 can improve the cardiac function of rats with CHF， which may be associated with its functions in promoting the neovascularization of ischemic myocardium， reducing ischemic injury， and protecting myocardial tissue structure via up-regulation of the serum VEGF content and the expressions of Notch1 and DLL4 protein.关键词：Electroacupuncture;Chronic heart failure;Cardiac function;Myocardial neovascularization;Neurogenic site-gap homologous protein （Notch） signaling pathway6|0|0更新时间：2026-01-21
- “最新研究发现，艾灸“肺俞”“心俞”能降低心肌NCOA4、DMT1表达，抑制NCOA4与LC3B结合，改善铁代谢紊乱，抑制铁死亡，减缓CHF心肌纤维化进程。”摘要：ObjectiveTo observe the effect of moxibustion at “Feishu” （BL13） and “Xinshu” （BL15） on cardiac nuclear receptor co activator 4 （NCOA4）， divalent metal transporter 1 （DMT1） and type Ⅲ collagen cardiac collagen fibers （Collagen Ⅲ） in rats with chronic heart failure （CHF）， so as to explore its underlying mechanisms in improvement of CHF.MethodsMale SD rats were randomly divided into the normal （n=10）， model （n=5）， moxibustion （n=7）， rapamycin （n=4）， and moxibustion + rapamycin （n=5） groups. The CHF model was established by permanent ligation of the anterior descending branch of the left coronary artery. In the moxibustion and moxibustion + rapamycin group， mild moxibustion was applied to bilateral BL13 and BL15 for 15 min once daily for 4 weeks. In the rapamycin and moxibustion + rapamycin group， rapamycin solution （1 mg/kg） was intraperitoneally injected once a day for 4 weeks. Behavioral observations were conducted on rats in each group. The ejection fraction （EF） and left ventricular fractional shortening rate （FS） were examined by echocardiography. Masson staining and transmission electron microscopy were used to observe the structural morphology of the rats’ heart. Immunofluorescence colocalization was used to observe the colocalization of NCOA4 and autophagy microtubule-associated protein light chain 3B （LC3B）. Real-time fluorescent quantitative PCR was used to detect the mRNA expressions of NCOA4， DMT1， and Collagen Ⅲ in the myocardial tissues of rats in each group， and Western blot was used to detect the protein levels of DMT1 and Collagen Ⅲ.Results（1） After modeling， the mitochondrial cristae of cardiomyocytes were disrupted or even disappeared， the EF and FS values were decreased （P<0.01）， while the blue-stained area of myocardial fibers， the colocalization of NCOA4 and LC3B， the mRNA expression level of NCOA4， the protein and mRNA expression levels of DMT1 and Collagen Ⅲ were all increased （P<0.01） in the model group compared with the normal group. （2） Compared with the model group， the morphological damage of mitochondria was improved， the EF and FS values were increased （P<0.05， P<0.01）， whereas the blue-stained area of myocardial fibers， the colocalization of NCOA4 and LC3B， the mRNA expression level of NCOA4， and the protein and mRNA expression levels of DMT1 and Collagen Ⅲ were decreased （P<0.01） in the moxibustion group. At the same time， the blue-stained area of myocardial fibers， the colocalization of NCOA4 and LC3B， the mRNA expression level of NCOA4， and the protein and mRNA expression levels of DMT1 and Collagen Ⅲ were all increased （P<0.01） in the rapamycin group. （3） Compared with the moxibustion group， all these indicators showed opposite trends （P<0.01， except EF and FS） in the moxibustion + rapamycin group.ConclusionMoxibustion can reduce the expression of NCOA4 and DMT1， inhibit the combination of NCOA4 and LC3B， thereby antagonizing ferritin autophagy， improving iron metabolism disorders， inhibiting ferroptosis and down-regulating Collagen Ⅲ expression， ultimately slowing the process of CHF myocardial fibrosis.关键词：Chronic heart failure;Moxibustion;Nuclear receptor coactivator 4;Divalent metal transporter protein 1;Myocardial fibrosis51|96|0更新时间：2026-01-21
- “最新研究发现，电针刺激“犊鼻”“足三里”穴位能抑制滑膜组织自噬，改善膝关节骨关节炎大鼠关节功能和滑膜组织结构，可能通过激活PI3K/Akt/mTOR信号通路发挥作用。”摘要：ObjectiveTo explore the effect of electroacupuncture （EA） via regulating the phosphatidylinositol 3-kinase （PI3K）/protein kinase B （Akt）/mammalian target of rapamycin （mTOR） signaling pathway on autophagy of synovial tissue in rats with knee osteoarthritis （KOA）.MethodsThirty-six male Sprague-Dawley （SD） rats were randomly divided into a normal group， a model group， and an EA group， with 12 rats in each group. The KOA model was replicated by injection of 50 μL sodium monoiodoacetate （MIA） into the right knee joint cavity. After successful establishment of the model， rats in the EA group received EA treatment at “Dubin” （ST35） and “Zusanli” （ST36） on the affected side， 20 min each time， once a day for 3 consecutive weeks. The Lequesne MG behavioral scale was used to evaluate the knee joint function of rats. The pathological morphology of the synovial tissue was observed after hematoxylin-eosin （HE） staining. The ultrastructure of autophagosomes and autolysosomes in the synovium was observed under transmission electron microscopy. The contents of autophagy-related protein 5 （Atg5） and autophagy-related protein 12 （Atg12） in the synovial tissue were detected by ELISA method. The expression levels of PI3K， Akt， and mTOR mRNA in the synovial tissue were detected by qPCR. The relative expression levels of PI3K， Akt， mTOR， UNC-51-like kinase 1 （ULK1）， Beclin-1， and microtubule-associated protein light chain 3 （LC3） protein in the synovial tissue were detected by Western blot.ResultsCompared with the normal group， the Lequesne MG score of the knee joint was increased （P<0.01） in the model group； the synovial collagen fiber proliferated， inflammatory cell infiltration occurred， the number of autophagosomes and autolysosomes increased； contents of Atg5， Atg12， and relative expressions of ULK1， Beclin-1 protein and LC3Ⅱ/LC3Ⅰ ratio in the synovial tissue were increased （P<0.01）， while the expressions of PI3K， Akt， and mTOR mRNA and protein were decreased （P<0.01）. Compared with the model group， the Lequesne MG score of the knee joint in the EA group was decreased （P<0.01）； the proliferation of synovial collagen fiber was alleviated and they were arranged neatly， the inflammatory cell infiltration was reduced， the number of autophagosomes and autolysosomes was decreased； contents of Atg5， Atg12， and the relative expressions of ULK1， Beclin-1 proteins and LC3Ⅱ/LC3Ⅰ ratio in the synovial tissue were decreased （P<0.01）， while the expressions of PI3K， Akt， and mTOR mRNA and protein were increased （P<0.05， P<0.01）.ConclusionEA at ST35 and ST36 can inhibit the autophagy of synovial tissue， improve the knee joint function and the morphological structure of synovial tissue in KOA rats， and its mechanism may be related to the activation of PI3K/Akt/mTOR signaling pathway.关键词：Knee osteoarthritis;Electroacupuncture;Autophagy;PI3K/Akt/mTOR signaling pathway;Synovium63|73|0更新时间：2026-01-21
- “最新研究发现，针刀疏筋解结术能有效减轻胶原诱导性关节炎大鼠的炎性反应和骨破坏，可能通过调控OPG/RANKL/NF-κB信号通路抑制破骨细胞分化。”摘要：ObjectiveTo explore the mechanism of acupotomy in inhibiting bone destruction in rats with collagen-induced arthritis（CIA）.MethodsTwenty-four SD female rats were randomly allocated to blank control， model and acupotomy groups， with 8 rats in each group. The CIA model was established by subcutaneous injection of bovine type II collagen + Freund’s incomplete adjuvant at the base of the tail （on day 0 and 7）. The rats of the acupotomy group received alternative relaxing treatment of Tongbi Qiji technique （sternum-xiphoid） and Tongyang Qishu technique （the region around Dazhui［GV14］） with needle knife， once every 3 d， for a total of 9 sessions. The rats of the blank control and model groups received grabbing and fixing as those of the acupotomy group. After the intervention， the arthritis index （AI） score （0—4 points for each limb， 16 points in total） was evaluated. Micro-CT was used to observe the bone destruction of the ankle joint and to analyze the related bone parameters including bone mineral density （BMD）， bone surface/bone volume （BS/BV）， bone volume/ total volume （BV/TV）， bone trabecular number （Tb.N）， trabecular thickness （Tb.Th） and trabecular separation/spacing （Tb.Sp）. The tartrate-resistant acid phosphatase （TRAP） staining was used to observe changes of the number of osteoclast （OC） in the ankle tissue， and the HE staining was used to observe histopathological changes of the ankle joint. The contents of serum tumor necrosis factor-α （TNF-α）， interleukin （IL） -6 and IL-1β were detected by ELISA. The mRNA and protein expression levels of osteoprotegin （OPG）， receptor activator of nuclear factor-κB ligand （RANKL）， nuclear factor-κB （NF-κB） p65， and nuclear factor-κB inhibitory protein kinase β （IKKβ） in the ankle tissue were detected by real-time fluorescent quantitative PCR and Western blot， respectively.ResultsCompared with the blank control group， the AI score， BS/BV， Tb.Sp， serum contents of TNF-α， IL-6 and IL-1β， and the expression levels of RANKL， NF-κB p65 and IKKβ mRNA and protein were significantly increased （P<0.01）， while BMD， BV/TV， Tb.Th and Tb.N， and the expression levels of OPG mRNA and protein were all significantly decreased in the model group （P<0.01）. After acupotomy intervention， both the increase of the AI score from day 14 to 28 after intervention， BS/BV， Tb.Sp， serum contents of TNF-α， IL-6 and IL-1β， and the expression levels of RANKL， NF-κB p65 and IKKβ mRNA and protein， and the decrease of BMD， BV/TV， Tb.Th and Tb.N， and the expression levels of OPG mRNA and protein were reversed in the acupotomy group （P<0.01， P<0.05）. Micro-CT showed rough surface of the ankle joint， enlarged gap， significantly reduced bone mass and serious bone erosion in the model group， and improved surface density of the ankle joint， increased bone mass and decreased bone erosion in the acupotomy group. HE staining displayed an obvious proliferation of synovial cells in the ankle joint tissue， and infiltration of a large number of inflammatory cells in the model group； but no apparent proliferation of the synovial cells in the ankle joint tissue， and only a small amount of inflammatory cells in the acupotomy group.ConclusionAcupotomy can reduce inflammation， inhibit bone destruction in RA rats， which may be related to its function in regulating the OPG/RANKL/NF-κB signaling pathway， further inhibiting OC differentiation.关键词：Acupotomy tendon-sparing and knot-dissolving technique;Rheumatoid arthritis;OPG/RANKL/NF-κB signaling pathway;Bone destruction33|44|0更新时间：2026-01-21
- “最新研究发现，针刺“关元”“三阴交”能改善卵巢储备功能减退大鼠的卵巢储备功能，可能通过抑制KEAP1/PGAM5/AIFM1通路，促进卵巢血管生成，改善卵巢血流灌注。”摘要：ObjectiveTo observe the effect of acupuncture of “Guanyuan” （CV4）-“Sanyinjiao” （SP6） on ovarian angiogenesis in rats with diminished ovarian reserve （DOR） by regulating the kelch-like ECH-associated protein 1 （KEAP1）/phosphoglycerate mutase 5 （PGAM5）/mitochondrial apoptosis-inducing factor 1 （AIFM1） pathway， so as to explore its mechanisms underlying improvement of DOR.MethodsEighteen female SD rats were randomly divided into blank， model， and acupuncture groups， with 6 rats in each group. The DOR model was established by a single intraperitoneal injection of 75 mg/kg cyclophosphamide. After modeling， the rats of the acupuncture group received manual acupuncture stimulation of CV4 and SP6 for 1 min， with the needle retained for 20 min， once every other day， for a total of 15 times. Vaginal exfoliated cytology was used to observe the rat’s estrous cycle. The levels of follicle-stimulating hormone （FSH）， luteinizing hormone （LH）， estradiol （E2）， and anti-Mullerian hormone （AMH） in the serum were detected by ELISA. The ovarian area and the peak systolic velocity （PSV）， end-diastolic velocity （EDV）， resistance index （RI）， and pulsatility index （PI） of the ovarian artery were estimated using a small animal ultrasonography， and the PSV/EDV （S/D） was calculated. Morphological changes of ovarian tissue and follicle growth were observed by H.E. staining， and the level of reactive oxygen species （ROS） in the ovarian tissue was detected by flow cytometry. The expression levels of vascular endothelial growth factor （VEGF）， platelet endothelial cell adhesion molecule 1 （CD31）， KEAP1， PGAM5 and AIFM1 in the ovarian tissue were detected by immunofluorescence staining.ResultsCompared with the blank group， the model group had a significant decrease in the ovarian area， serum E2 and AMH contents， numbers of the primordial follicles， secondary follicles and mature follicles， and the levels of VEGF and CD31（P<0.05）， and a striking increase in the PI， RI and S/D of the ovarian artery， serum FSH and LH contents， ovarian ROS， immunoactivity of ovarian KEAP1， PGAM5 and AIFM1（P<0.05）. In comparison with the model group， both the decrease and increase of the indexes mentioned above were significantly reversed in the acupuncture group （P<0.05）， except the number of mature follicles. H.E. staining showed structural disorder， reduction of the vascular distribution and the number of primordial follicles， secondary follicles and mature follicles in the ovarian tissues of the model group， which was relatively milder in the injury degree in the acupuncture group， including clearer ovarian tissue structure， and increase of the distribution of blood vessels， and the number of primary and secondary follicles.ConclusionAcupuncture of CV4 and SP6 can improve the ovarian reserve function of DOR rats， which may be related to its functions in inhibiting the KEAP1/PGAM5/AIFM1 pathway of ovarian tissue， promoting ovarian angiogenesis， and ultimately improving blood perfusion.关键词：Acupuncture;Diminished ovarian reserve;Angiogenesis;KEAP1/PGAM5/AIFM1 pathway39|24|0更新时间：2026-01-21
- “最新研究发现，艾灸能延缓小鼠卵巢功能减退，调节性激素水平，可能通过改善线粒体功能实现。”摘要：ObjectiveTo investigate the effect of moxibustion on delaying ovarian aging in naturally aging mice and to explore the underlying mechanisms.MethodsFifteen 2-month-old female SPF ICR mice with regular estrous cycles were included in a 2-month-old group. Thirty 10-month-old female SPF ICR mice were randomly divided into a 10-month-old group and a moxibustion group， with 15 mice in each group. The moxibustion group received alternating moxibustion on bilateral “Shenshu”（BL23） and “Zhongwan” （CV12）， “Guanyuan” （CV4） for 10 min every other day， for 21 consecutive days. The vaginal smears were used to observe the estrous cycle irregularities of mice. The serum contents of estradiol （E2）， follicle-stimulating hormone （FSH）， and anti-Müllerian hormone （AMH） were measured by ELISA. The ovarian morphology was observed by hematoxylin-eosin staining. The contents of adenosine triphosphate （ATP） in granulosa cells were measured by luminescence-based ATP assay. The levels of mitochondrial membrane potential （MMP） of granulosa cells were measured by JC-1 staining. The real-time fluorescence quantitative PCR， Western blot， and immunohistochemistry were used to assess the mRNA and protein expression levels of mitochondrial unfolded protein response （UPRmt）-related signaling pathway components， including activating transcription factor 5 （ATF5）， caseinolytic protease P （ClpP）， and Lon protease 1 （LONP1） in the ovarian tissues.ResultsCompared with the 2-month-old group， the 10-month-old group exhibited an increased rate of estrous cycle irregularity （P<0.01）， elevated serum FSH contents （P<0.01）， decreased E2 and AMH contents （P<0.01）， reduced number of follicles at all stages （P<0.01）， reduced granulosa cell ATP content and MMP level （P<0.01）， decreased positive expression levels of ATF5， ClpP， and LONP1 protein， and diminished expression levels of ATF5， ClpP， and LONP1 mRNA and protein in ovarian tissue （P<0.01）. Compared with the 10-month-old group， the moxibustion group showed a lower rate of estrous cycle irregularity （P<0.01）， decreased serum FSH content （P<0.01）， increased E2 and AMH contents （P<0.01）， increased the number of primordial， growing and antral follicles （P<0.05， P<0.01）， elevated granulosa cell ATP content and MMP level （P<0.01）， increased ATF5， ClpP， and LONP1 protein positive expression levels， and enhanced ATF5， ClpP， and LONP1 mRNA and protein expression levels in the ovarian tissue （P<0.01， P<0.05）.ConclusionMoxibustion can delay ovarian dysfunction related to natural aging in mice and modulate hormonal levels. Its mechanism may involve regulating the expressions of UPRmt-related signaling molecules such as ATF5， ClpP and LONP1， and enhancing mitochondrial function in granulosa cells.关键词：Ovarian aging;Mitochondrial function;Moxibustion;Mitochondrial unfolded protein response;Activating transcription factor 540|36|0更新时间：2026-01-21
- “最新研究发现，电针足少阳经穴通过激活PI3K/AKT信号通路，有效减轻绝经后骨质疏松大鼠的炎性反应和骨质流失。”摘要：ObjectiveTo investigate the effect of electroacupuncture （EA） at Foot Shaoyang meridian acupoints on the inflammatory response in postmenopausal osteoporosis rats by regulating the phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT） signaling pathway.MethodsForty Sprague-Dawley （SD） rats were randomly divided into a sham operation group （n=10）， and the remaining rats underwent bilateral ovariectomy to establish a postmenopausal osteoporosis model after 3 months. The model rats were further randomized into a model group， an EA group， and an EA + inhibitor group （n=10）. Rats of The EA group received EA at bilateral “Huantiao” （GB30）， “Yanglingquan” （GB34） （dense-sparse wave， 2 Hz/15 Hz， 0.2—0.6 mA）， and acupuncture at “Xuanzhong” （GB39）， 10 min each time， 5 times a week for 8 weeks， with alternate left-right acupoints. Rats of the EA + inhibitor group received intraperitoneal injection of the PI3K inhibitor LY294002 （10 mg/kg） twice a week for 8 weeks based on the EA treatment. Micro-CT was used to detect the microstructural and morphometric indices of the femoral bone tissue， including bone mineral density （BMD）， bone volume fraction （BV/TV）， trabecular number （Tb.N）， and trabecular separation （Tb.Sp）. HE staining was performed to observe the pathological morphology of the femoral tissue， and tartrate-resistant acid phosphatase （TRAP） staining was used to observe the number of osteoclasts. Immunohistochemical staining was employed to detect the expressions of osteogenic markers Runt-related transcription factor 2 （Runx2）， osteocalcin （OCN）， and type I collagen （Collagen I）. The contents of serum interleukin-1β （IL-1β）， interleukin-6 （IL-6）， and tumor necrosis factor-α （TNF-α）， as well as their mRNA expressions in bone tissue， were detected by ELISA and real-time fluorescent quantitative PCR， respectively. Western blot was used to detect the protein expressions of the PI3K/AKT pathway in femoral tissue.ResultsIn the model group， bone trabeculae were significantly thinned， sparsely and disorderly arranged， the bone marrow cavity was expanded， and the number of osteoclasts was significantly increased； the EA group showed improved bone trabecular structure and reduced osteoclast number. Compared with the sham operation group， the model group had significantly lower BMD， BV/TV， Tb.N， positive expressions of Runx2， OCN， and Collagen I in bone tissue， and ratios of p-PI3K/PI3K and p-AKT/AKT protein expression （P<0.01）， while Tb.Sp， serum IL-6， IL-1β， TNF-α contents and their mRNA expressions in bone tissue were significantly higher （P<0.01）. Compared with the model group， the EA group had significantly higher BMD， BV/TV， Tb.N， positive expressions of Runx2， OCN， and Collagen I in bone tissue， and ratios of p-PI3K/PI3K and p-AKT/AKT protein expression （P<0.01， P<0.05）， while Tb.Sp， serum IL-6， IL-1β， TNF-α contents and their mRNA expressions in bone tissue were significantly lower （P<0.01）. Compared with the EA group， all the above indices except Tb.Sp were reversed in the EA + inhibitor group （P<0.01， P<0.05）.ConclusionEA at Foot Shaoyang meridian acupoints can alleviate inflammatory responses and bone loss in postmenopausal osteoporosis rats， and its mechanism may be related to the activation of the PI3K/AKT pathway.关键词：Electroacupuncture;Foot Shaoyang meridian acupoints;Phosphatidylinositol 3-kinase /protein kinase B pathway;Osteoporosis;Inflammatory response23|11|0更新时间：2026-01-21
- “最新研究发现，电针干预能通过调节糖酵解/H4K12la/HIF-1α信号环路，改善海马区炎性反应，缓解创伤后应激障碍大鼠焦虑行为。”摘要：ObjectiveTo observe the effect of electroacupuncture （EA） on anxiety-like behavior and activity of glycolysis/histone H4 lysine 12 lactylation （H4K12la）/hypoxia-inducible factor 1α （HIF-1α） signaling in rats with post-traumatic stress disorder （PTSD）， so as to explore its underlying molecular mechanisms in the improvement of PTSD-induced anxiety-like behavior.MethodsMale SD rats were randomly divided into control， PTSD model， and EA groups， with 10 rats in each group. The PTSD model was established by using a modified single prolonged stress and shock （SPS&S） protocol. The rats in the EA group received EA stimulation （2 Hz/100 Hz， 1 mA） at bilateral “Ganshu”（BL18） and “Shenshu”（BL23）， and acupuncture at “Baihui”（GV20）， “Shenting” （GV24）. The anxiety-like behavior was assessed by using both open field （OF） test and elevated plus maze （EPM） test， separately. Morphological changes of hippocampal neurons were observed by HE staining. Glycolysis-related metabolites including lactate， glucose， ATP and pyruvate were measured using biochemical kits. The expressions of H4K12la， HIF-1α， and key glycolytic enzymes ［lactate dehydrogenase （LDHA）and pyruvate kinases type M2 （PKM2） ］ in the hippocampal tissue were evaluated by qPCR and Western blot， separately. The spatial distribution of H4K12la and HIF-1α in the hippocampus was further analyzed by immunohistochemistry （IHC）.ResultsThe anxiety-like behavior tests showed that the percentages of entries and time spent in the open arms of the EPM test， the total distance and the distance travelled in the center zone of OF test were apparently lower in the model group than in the control group （P<0.01）， and significantly higher in the EA group than in the model group （P<0.01）. In contrast to the control group， the model group displayed a decrease in the ATP content （P<0.01）， and an increase in the contents of glucose， lactic acid， and the expression levels of PKM2 and LDHA mRNAs and proteins， and H4K12la and HIF-1α proteins and immunoactivity in the hippocampal tissues （P<0.05， P<0.01）. After EA intervention， the decreased level of ATP and the increased levels of glucose， lactic acid， and expression levels of PKM2 and LDHA mRNAs and proteins， and H4K12la and HIF-1α proteins and immunoactivity were reversed （P<0.05， P<0.01）. No significant changes were found in the levels of pyruvate， and expression of H4K12la and HIF-1α mRNAs after modeling and after EA intervention. H.E. staining showed that many hippocampal pyramidal cells were shriveled， with irregular cell shapes， reduced volume and a small number of tiny vacuoles in the model group， while a large number of pyramidal cells were found to have regular shapes， neat and dense arrangement， and a few of shrunken pyramidal cells in the EA group.ConclusionEA intervention can improve the anxiety-like behavior in PTSD rats， which may be related to its functions in down-regulating the glycolysis/H4K12la/HIF-1α pathway and mitigating inflammation in the hippocampus.关键词：Electroacupuncture;Post-traumatic stress disorder;Anxiety-like behavior;Glycolysis;Histone lactylation53|48|0更新时间：2026-01-21
- “最新研究发现，基于“标本根结”理论的针刺疗法能有效改善外展神经麻痹患者的复视、斜视等症状，促进功能重建。”摘要：ObjectiveTo observe the clinical effect of acupuncture on abducens nerve palsy （ANP） guided by Biaoben Genjie theory （referring to the distribution， connection and gathering of meridian qi in the body）.MethodsA total of 60 patients with ANP were randomly assigned to an observation group （30 cases） and a control group （30 cases）. The conventional treatment with western medicine was used in the two groups. Additionally， in the observation group， based on Biaoben Genjie theory， acupuncture therapy was delivered at the sites of Biao， Ben， Gen and Jie of Shaoyang Meridians of hand and foot， meaning at Sizhukong （TE23）， Zhongzhu （TE3）， Zuqiaoyin （GB44） and Tongziliao （GB1）， combined with Fengchi （GB20）， Taiyang （EX-HN5）， Qiuhou （EX-HN7） and Guangming （GB37）. In the control group， the routine acupuncture therapy was operated. In either group， the intervention was given once daily， and 6 interventions a week as 1 course of treatment； 6 courses were required， with a 1- day interval after every 6 interventions. Before and after treatment， diplopia degree， strabismus degree， the score for eye position， and the score for main symptoms of traditional Chinese medicine （TCM） were recorded and the clinical effect was evaluated in the two groups.ResultsAfter treatment completion， diplopia degree， strabismus degree， the score for eye position， and the score for main symptoms of TCM were reduced in both of the two groups compared with those before treatment （P<0.05）， and the results in the observation group were better than those of the control group （P<0.05）. The effective rate was 96.67% （29/30） in the observation group， and it was 86.67% （26/30） in the control group. The effective rate in the observation group was superior to that in the control group （P<0.05）.ConclusionUnder the guidance of Biaoben Genjie theory， acupuncture therapy can effectively ameliorate diplopia degree， strabismus degree， eye position score and the main symptoms of TCM； and it can promote the functional reconstruction in ANP to a certain extent.关键词：Abducens nerve palsy;Biaoben Genjie theory;Acupuncture;Clinical observation61|131|0更新时间：2026-01-21
- “最新研究显示，热敏灸能有效降低恶性肿瘤化疗患者的不良反应，提高化疗效果。”摘要：ObjectiveTo observe the clinical efficacy of heat-sensitive moxibustion in reducing toxicity and enhancing efficacy in cancer patients undergoing chemotherapy， and to provide a scientific basis for its application and promotion in cancer rehabilitation.MethodsA total of 150 malignant tumor patients were selected and randomly divided into a control group （75 cases， with 25 dropouts） and an experimental group （75 cases， with 21 dropouts）. The control group received conventional chemotherapy and pharmacological treatment， while the experimental group received additional heat-sensitive moxibustion treatment， which administered once daily for 1 week before and after each chemotherapy session， for 4 consecutive chemotherapy cycles. Evaluation indicators included the incidence of adverse reactions， anorexia/cachexia scale （A/CS-12）， nausea and vomiting grading， white blood cell count， platelet count， hemoglobin level， bone marrow suppression grade， and response evaluation criteria in solid tumors （RECIST 1.1）. Data were collected before and after each chemotherapy session and statistically analyzed.ResultsCompared with pre-chemotherapy levels， as the number of chemotherapy sessions was increased， A/CS-12 scores were decreased in both groups （P<0.01）， while the incidence of nausea and vomiting increased （P<0.01）， the white blood cell count， platelet count， and hemoglobin levels were also decreased （P<0.01）. Intergroup comparisons showed that the experimental group had a lower incidence of adverse reactions after each chemotherapy session （P<0.05， P<0.01）， higher A/CS-12 scores （P<0.01）， and lower rates of nausea and vomiting and bone marrow suppression （P<0.05， P<0.01） than the control group. Hematological analysis indicated that the experimental group had higher white blood cell counts and hemoglobin levels before the 2nd to 4th chemotherapy sessions （P<0.05， P<0.01）， and higher platelet counts before the 3rd and 4th sessions （P<0.01）. RECIST evolution result showed that the experimental group had an efficacy rate of 50.00%（27/54）， which was higher （P<0.05） than that（28.00%［14/50］） of the control group.ConclusionHeat-sensitive moxibustion can reduce the incidence of chemotherapy-induced adverse reactions， alleviate gastrointestinal discomfort and bone marrow suppression， improve chemotherapy efficacy. It is worthy of clinical promotion and application.关键词：Heat-sensitive moxibustion;Malignant tumor;Chemotherapy;Toxicity reduction and efficacy enhancement61|36|0更新时间：2026-01-21
- “在针刺手法研究领域，专家应用光学定位系统建立了针刺手法波形采集与参数量化分析方法，为针刺手法客观量化研究提供解决方案。”摘要：ObjectiveTo establish a method for in vivo acquisition of acupuncture manipulation （AM） waveforms and objective quantitative analysis of acupuncture parameters based on the motion trajectory of filiform needle by applying an optical positioning system， so as to evaluate the accuracy of AM waveforms acquisition and feasibility of quantitative analysis performed by the method.MethodsFirstly， the Ruitong SE optical positioning system and a customized marking ball that can be connected to an acupuncture needle were employed to construct an in vivo AM acquisition method. The AM waveforms acquired by the optical positioning system and the AM parameter-measuring instrument were respectively compared and analyzed. Then， the waveforms of 6 types of AMs， namely， the lifting-thrusting uniform reinforcing-reducing method， lifting-thrusting reinforcing method， lifting-thrusting reducing method， twirling reinforcing-reducing method， twirling reinforcing method and twirling reducing method， were recorded in real time by stimulating Zusanli （ST36） in healthy volunteers. The AM parameters including the displacement and velocity of lifting and thrusting manipulations and the twisting angle and angular velocity of the twirling method were quantitatively analyzed.ResultsThe AM waveforms recorded by the optical positioning system were similar to the waveforms recorded by the AM parameter measuring instrument， which had a positive correlation （P<0.01）. There was no significant difference between the upward and downward displacements in the lifting-thrusting manipulations， and the clockwise and counterclockwise rotation angles in twirling manipulations. However， a significant difference was found in the movement velocity， that is， 1） the downward movement speed of the lifting-thrusting reinforcing method was greater than that of the lifting speed （P<0.001）， while the reduction method was opposite （P<0.001）； and 2） the clockwise twisting angle speed of the twirling reinforcing method was higher than that of the counterclockwise twisting angle speed （P<0.001）， and the reverse was true for the reduction method （P<0.001）.ConclusionThe AM waveforms recorded in vivo by using the optical positioning system are accurate， and can also be used for quantitative analysis of the AM parameters， which is applicable in the objective quantitative study of AMs.关键词：Acupuncture manipulation;Optical positioning system;Quantification analysis;Objective study80|85|0更新时间：2026-01-21
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