最新刊期
卷 51 ， 期 3 ， 2026
- “研究发现电针“内关”和“足三里”可调控心肌缺血大鼠心功能，其适宜刺激强度分别为1.5 mA和3 mA，且需自主神经系统参与。”摘要：ObjectiveTo screen the optimal stimulation intensity of electroacupuncture （EA） at “Neiguan” （PC6） and “Zusanli” （ST36） for regulating autonomic nerve activity and cardiac function in rats with myocardial ischemia （MI）， so as to explore the potential underlying mechanism.MethodsForty male Sprague-Dawley （SD） rats were randomly divided into a control group and a model group， with 20 rats in each group. The MI model was established by ligating the left anterior descending coronary artery （LAD）. Rats in the control group underwent thoracotomy without LAD ligation. Lead Ⅱ electrocardiogram （ECG） was used to monitor ST segment elevation； Masson staining was performed to measure the cardiac collagen volume fraction （CVF）； Doppler echocardiography was applied to assess ejection fraction （EF） and fractional shortening （FS）， respectively for model evaluation. At the 7th day after MI induction， EA with different intensities （0.5， 1.5， 3 mA） was administered at left PC6 and ST36 for 1 min in both the control and model groups. In vivo neuroelectrophysiological recording was used to detect the discharge frequency of the left cervical sympathetic nerve （SN） and cervical vagus nerve （VN）. ECG was employed to record heart rate （HR） and heart rate variability （HRV）， and the Millar pressure-volume conductance catheter system was used to measure stroke volume （SV）， stroke work （SW）， and cardiac output （CO）， so as to screen the optimal stimulation intensity for each acupoint. Autonomic denervation was performed in the model group （n=10）. Before and after denervation， EA with the optimal intensity was delivered at left PC6 and ST36， and the above indicators were measured repeatedly to verify whether the autonomic nervous system is involved in the regulatory effect of EA on cardiac function in MI rats.ResultsAfter LAD ligation， ECG showed a significant elevation of the ST segment （P<0.000 1）， accompanied by marked decreases in EF and FS （P<0.000 1） and an increase in CVF （P<0.01）， indicating successful establishment of the MI model. The basal discharge frequency of SN in the model group was significantly higher than that in the control group （P<0.05）. In the control group， 1.5 mA EA at PC6 resulted in an increased HR （P<0.01）. In the model group， 1.5 mA EA at PC6 led to increases in the discharge frequency of SN and VN， HRV， as well as SV， SW and CO （P<0.01， P<0.05， P<0.001）. 1.5 mA EA at ST36 increased the discharge frequency of VN in the model group （P<0.05）. In the control group，3 mA EA at ST36 induced an elevation of HR （P<0.05）； in the model group， the same intervention increased the discharge frequency of SN， along with SV， SW and CO （P<0.05，P<0.01）. After autonomic denervation， the regulatory effects of EA at the above acupoints with optimal intensities on cardiac function were abolished.ConclusionThe optimal intensity of EA at PC6 for improving cardiac function is 1.5 mA， while that at ST36 is 3 mA. The EA-induced regulatory effects depend on the integrity of the autonomic nervous system.关键词：Electroacupuncture;Myocardial ischemia;Autonomic nerve;Cardiac function;Neiguan （PC6）;Zusanli （ST36）48|89|0更新时间：2026-03-16
- “专家研究麦粒灸对围绝经期失眠大鼠的影响，发现其可改善睡眠，机制与减轻SIRT1/Nrf2通路介导的下丘脑氧化应激和细胞焦亡有关。”摘要：ObjectiveTo observe the effect of wheat-grain moxibustion（WGM） at “Guanyuan”（CV4）， “Mingmen”（GV4）， and bilateral “Xuanzhong”（GB39） on the oxidative stress and pyroptosis， and the activity of the Sirtuin 1（SIRT1）/nuclear factor erythroid-2-related factor 2 （Nrf2） signaling pathway in the hypothalamus of perimenopausal insomnia （PI） rats， so as to explore its underlying mechanism in improving PI.MethodsThirty-two female SD rats were randomly divided into four groups： sham， model， WGM， and WGM+EX527 （n=8 per group）. The PI model was established by bilateral ovariectomy combined with an intraperitoneal injection of 4-Chloro-DL-phenylalanine suspension （350 mg/kg） for 2 consecutive days. Rats in the WGM and WGM+EX527 groups received WGM at CV4， GV4 and GB39， with 6 cones for each acupoint. The rats in the WGM+EX527 group received additional intraperitoneal injection of the SIRT1 inhibitor EX527 （10 mg/kg） 30 min before each moxibustion session. The interventions were conducted once daily for 14 consecutive days. The sleep latency and sleep duration were assessed using the pentobarbital sodium-induced sleep test （righting reflex）， and the spontaneous activities （total distance moved， center zone dwelling time， and rearing times in 5 min） were measured using the open field test （OFT）. The hematoxylin-eosin （H.E.） staining was used to observe morphological changes in the hypothalamic tissue. Western blot was used to detect protein expression levels of SIRT1， Nrf2， Kelch-like ECH-associated protein1 （Keap1）， heme oxygenase-1 （HO-1）， superoxide dismutase （SOD）， catalase （CAT）， NOD-like receptor thermal protein domain associated protein 3 （NLRP3）， cysteingl aspartate specific protease 1 （Caspase-1）， apoptosis-associated speck-like protein containing a CARD （ASC）， and gasdermin-D （GSDMD） in the hypothalamus. The qPCR was used to measure mRNA expression levels of SIRT1， Nrf2， Keap1， and HO-1 in the hypothalamus. The reactive oxygen species （ROS） assay kit was used to detect ROS level of hypothalamic tissue. The ELISA was used to measure the content of interleukin （IL）-1β and IL-18 in the hypothalamus.ResultsCompared to the sham group， the model group showed a striking decrease in the total distance traveled， central zone dwelling time and rearing times in the OFT （P<0.01）， and significantly shortened sleep duration and prolonged sleep latency （P<0.01）. In comparison with the model group and the WGM+EX527 group， the WGM group showed an obvious increase in the total distance traveled， central zone dwelling time and rearing times （P<0.01）， and shortened sleep latency， prolonged sleep duration （P<0.01）. Additionally， in comparison with the sham group， the model group had a significant down-regulation in the expression levels of hypothalamic SIRT1， Nrf2， Keap1， and HO-1 proteins and mRNAs， and SOD and CAT proteins （P<0.01）， and an obvious up-regulation in the expression levels of NLRP3， Caspase-1， ASC， GSDMD proteins， ROS level， and contents of IL-1β and IL-18 （P<0.01） in the hypothalamus. In comparison with the model group and the WGM+EX527 group， both the down-regulation and up-regulation of expression levels of the proteins and mRNAs mentioned above， and ROS level and the contents of IL-1β and IL-18 were reversed in the WGM group（P<0.01，P<0.05）. H.E. staining showed that in comparison with the sham group， more hypothalamic neurons were wrinkled with unclear cell structures， varying sizes and uneven distribution in the model and WGM+EX527 groups， which was alleviated in the degree of cell injury in the WGM group， for instance clearer cell structure and uniform distribution.ConclusionWGM can improve the sleep and spontaneous activity of PI rats， which may be related to its functions in alleviation of hypothalamic oxidative stress and pyroptosis by regulating the SIRT1/Nrf2 signaling pathway.关键词：Perimenopause;Insomnia;Wheat-grain moxibustion;SIRT1;Nrf2;Hypothalamus17|69|0更新时间：2026-03-16
- ““电针可改善PD小鼠运动功能障碍，减少中脑黑质多巴胺能神经元的丢失，提高TH表达水平，其作用机制可能与调控SIRT3/NF-κB/MAPK信号通路抑制神经炎性反应有关。””摘要：ObjectiveTo investigate the effects of electroacupuncture （EA） at “Fengfu” （GV16） and “Taichong” （LR3） on tyrosine hydroxylase （TH）， microglia （MG）， and the silent information regulator 3（SIRT3）/nuclear factor-kappa B （NF-κB）/mitogen-activated protein kinase （MAPK） signaling pathway-related proteins in the substantia nigra of Parkinson’s disease （PD） mice， and to explore the potential mechanisms underlying the therapeutic effects of EA on PD.MethodsC57BL/6J mice were randomly divided into 4 groups： control group， model group， non-acupoint group， and EA group， with 12 mice in each group. A PD model was established by intraperitoneal injections of MPTP for 7 consecutive days. The EA group received EA at GV16 and LR3. The non-acupoint group underwent needle insertion 3 mm lateral to GV16 and LR3 without electrical stimulation. Treatments were administered for 15 min per session， once daily for 14 days. Motor function was assessed using the pole test and suspension test. Immunohistochemistry was used to detect TH-positive expression in the substantia nigra. Immunofluorescence was employed to quantify activated MG. Western blot was used to measure the protein expression levels of SIRT3， NF-κB p65， and p38 MAPK. ELISA was used to determine the serum levels of tumor necrosis factor-α （TNF-α） and interleukin-1β （IL-1β）.ResultsCompared with the control group， the model group exhibited a longer total time in the pole test （P<0.01）， a lower score in the suspension test （P<0.01）， a reduce in mean TH optical density in the substantia nigra （P<0.01）， an increase in the number of MG-positive cells （P<0.01）， a decrease in SIRT3 protein expression （P<0.01）. The protein expression levels of NF-κB p65 and p38 MAPK proteins in the substantia nigra， and the serum levels of TNF-α， and IL-1β were all elevated （P<0.01）. Compared with model group and the non-acupoint group， the EA group showed a shortened total time in the pole test （P<0.01）， a higher score in the suspension test （P<0.01）， an increase in mean TH optical density in the substantia nigra （P<0.01）， a decrease in the number of MG-positive cells （P<0.01）， an increase in SIRT3 protein expression （P<0.05）. The expression levels of NF-κB p65 and p38 MAPK proteins in the substantia nigra， and the serum contents of TNF-α and IL-1β were all decreased （P<0.01）.ConclusionEA can improve motor dysfunction in PD mice， reduce the loss of dopaminergic neurons， and increase TH expression in the substantia nigra. The underlying mechanism may involve the modulation of the SIRT3/NF-κB/MAPK signaling pathway to suppress neuroinflammation.关键词：Parkinson’s disease;Electroacupuncture;Microglia;Silent information regulator 3;Nuclear factor-kappa B;Mitogen-activated protein kinase34|57|0更新时间：2026-03-16
- “研究发现益元灸通过NRF2/GPX4通路减轻脊髓神经元铁死亡，改善脊髓损伤大鼠尿潴留，为相关治疗提供新思路。”摘要：ObjectiveTo investigate the mechanism of Yiyuan moxibustion in improving lipid peroxidation and urine retention in spinal cord injury （SCI） rats by regulating ferroptosis via the nuclear factor erythroid 2-related factor 2 （NRF2）/glutathione peroxidase 4 （GPX4） pathway.MethodsForty-eight female Wistar rats were randomly divided into sham operation， model， moxibustion and moxibustion + inhibitor （combination） groups， with 12 rats in each group. SCI induced urinary retention model was established by using Allen’s method. Moxibustion was applied to “Shenque” （CV8）， “Guanyuan”（CV4） and “Zhongji” （CV3） for 15 min， once daily for 14 d. Rats in the combination group received moxibustion combined with intraperitoneal injection of the NRF2 inhibitor ML385 （30 mg/kg） once daily for 14 consecutive days. Urodynamic analysis was used to record bladder leak point pressure， maximum bladder volume， and bladder compliance to evaluate bladder function. H.E. staining was used to observe morphological changes in spinal cord tissue. Prussian blue staining was used to observe iron deposition in spinal cord tissue. Transmission electron microscopy （TEM） was used to observe mitochondrial morphological changes in spinal cord tissue. Colorimetric assays were used to measure serum Fe2+， malondialdehyde （MDA）， and reduced glutathione （GSH） levels. Western blot was used to detect the protein expressions of NRF2， GPX4， and solute carrier family 7 member 11 （SLC7A11） in spinal cord tissue.ResultsRats in the model group had disrupted spinal architecture， vacuolated neurons， swollen mitochondria with lost cristae， and brownish iron deposits， which were relatively milder in the moxibustion group. Compared with the sham operation group， the bladder leak point pressure， serum GSH content， and expression levels of NRF2， SLC7A11， and GPX4 proteins in spinal cord tissue were all significantly reduced （P<0.01）， while the maximum bladder volume and bladder compliance， as well as the serum contents of Fe2+ and MDA， were significantly increased （P<0.01） in the model group. When compared to the model group and the combination group， the moxibustion group exhibited a decrease in maximum bladder volume， bladder compliance， serum Fe2+ and MDA content （P<0.01）， but an increase in bladder leak point pressure， GSH content， and the expression of NRF2， SLC7A11， and GPX4 proteins （P<0.01，P<0.05）.ConclusionYiyuan moxibustion may alleviate lipid peroxidation caused by ferroptosis in spinal neurons by up-regulating the NRF2/GPX4 pathway， thereby promoting the recovery of neural regulation of bladder function and improving urine retention in SCI rats.关键词：Spinal cord injury;Yiyuan Moxibustion;Ferroptosis;Lipid peroxidation15|5|0更新时间：2026-03-16
- “研究发现针刺可改善脑缺血再灌注损伤大鼠脑损伤，减少铁死亡和铁自噬，其机制可能与KAT3B/ACSL4途径失活有关。”摘要：ObjectiveTo observe the effects of acupuncture on the KAT3B/ACSL4 pathway in the cerebral cortex of cerebral ischemia-reperfusion injury （CIRI） rats， and to explore the mechanisms by which acupuncture mitigates ferroptosis and ferritinophagy in CIRI.MethodsForty male SD rats were randomly divided into sham operation， model， acupuncture， and western medicine groups， with 10 rats in each group. The CIRI rat model was established by middle cerebral artery occlusion. The rats in the acupuncture group received acupuncture at “Baihui” （GV20）， “Sishencong” （EX-HN1）， and “Shuigou” （GV26） acupoints， with the needles retained for 30 min once daily for 7 consecutive days. The rats in the western medicine group received intraperitoneal injections of 0.29 mL·100 g-1·d-1 edaravone dexborneol for 7 consecutive days. Neurological deficit was assessed using the neurological deficit score. Infarct volume was measured by 2，3，5-triphenyltetrazolium chloride （TTC） staining. Blood-brain barrier permeability was assessed by Evans blue assay. Hematoxylin-eosin （HE） staining and Nissl staining were applied to assess histopathological alterations in the ischemic cerebral cortex. TUNEL staining was used for apoptosis detection in the ischemic cerebral cortex. Immunofluorescence staining was performed to detect the expression levels of neuronal nuclei （NeuN）， nuclear receptor coactivator 4 （NCOA4）， and autophagy-related protein light chain 3B （LC3B） in the ischemic cerebral cortex. Dihydroethidium （DHE） fluorescence probe was used to measure reactive oxygen species （ROS） levels in the ischemic cerebral tissue. Colorimetric assay was used to quantify the contents of ferrous iron （Fe2+）， lipid peroxidation （LPO）， malondialdehyde （MDA）， and glutathione （GSH）， along with superoxide dismutase （SOD） activity in the ischemic cerebral tissue. Transmission electron microscopy was used to assess mitochondrial damage in neurons of the ischemic cerebral cortex. Western blot was performed to evaluate the protein expression levels of KAT3B， ACSL4， glutathione peroxidase 4 （GPX4）， solute carrier family 7 member 11 （SLC7A11）， NCOA4， transferrin receptor 1 （TfR1）， ferritin heavy chain 1 （FTH1）， and ferroportin-1 （FPN-1） in the ischemic cerebral tissue.ResultsCompared with the sham operation group， the model group showed significant increases in neurological deficit score， cerebral infarction volume， and Evans blue permeability （P<0.01）； neuronal structure damage， with cell necrosis， nuclear shrinkage， loss of cytoplasm， and a decrease in Nissl bodies （P<0.01）. TUNEL-positive cells were increased （P<0.01）. Mitochondrial integrity was lost， mitochondria enlarged with cristae rupture and vacuolization. Expression of NeuN was decreased， while NCOA4 and LC3B expressions were increased （P<0.01）. In the ischemic cerebral tissue， contents of ROS， Fe2+， LPO， and MDA were increased， as well as the protein expression levels of KAT3B， ACSL4， NCOA4， and TfR1 were elevated （P<0.01）. By contrast， GSH content， SOD activity， and the expressions of GPX4， SLC7A11， FTH1， and FPN-1 were reduced （P<0.01）. Compared with the model group， the acupuncture and western medicine groups demonstrated reversal of these indicators （P<0.05）. There was no statistically significant difference between the acupuncture and western medicine groups.ConclusionAcupuncture can improve cerebral injury in CIRI rats and reduce ferroptosis and ferritinophagy， potentially through inactivation of the KAT3B/ACSL4 pathway.关键词：Acupuncture;Cerebral ischemia-reperfusion;Ferroptosis;Ferritinophagy;KAT3B/ACSL4 pathway56|65|0更新时间：2026-03-16
- “研究发现，不同频率电针“足三里”可改善IBS小鼠状态，降低结肠CRFR1、CRFR2与肥大细胞阳性共表达及下丘脑中CRF、CRFR1蛋白表达，增加下丘脑中CRFR2蛋白表达，变频电针效果更佳。”摘要：ObjectiveTo observe the effect of different stimulation frequencies of electroacupuncture （EA） of “Zusanli”（ST36） on the general state， visceral sensitivity， anxiety and depression， adrenocorticotropin releasing factor （CRF）， adrenocorticotropin releasing factor receptor 1 （CRFR1）， CRFR2 and mast cells in mice with irritable bowel syndrome （IBS）， so as to explore its mechanism underlying the improvement of IBS.MethodsMale C57BL/6 mice were randomly divided into blank， model， 2 Hz-EA， 15 Hz-EA and 2 Hz/15 Hz-EA groups， with 9 mice in each group. The IBS model was established by chronic unpredictable stress. EA （1 mA） was applied to bilateral ST36 for 15 min， once a day for 7 consecutive days. The general state， abdominal withdrawal reflex （AWR） scores for injection of air （0.25， 0.35 and 0.50 mL） into the colorectum-inserted air-balloon （for evaluating the visceral sensitivity）， open field test （OFT， for assessing the state of anxiety and depression） were observed. Morphological structure of mouse colon tissue was observed by HE staining， and the number of mast cells in the colon was quantified by toluidine blue staining. The number of CRFR1/tryptase- and CRFR2/tryptase-positive mast cells in the colon tissue was observed by immunofluorescence double labeling. The protein expression levels of CRF， CRFR1 and CRFR2 in the colon and hypothalamus tissues were measured by Western blot.ResultsMild erosion and edema in the colonic submucosa， and obvious inflammatory cell infiltration were observed in the model group， which was alleviated in each EA treatment group. Compared with the blank group， the mice in the model group showed listlessness， easy irritation， loose stool attached around the anus， and an increase in the AWR scores at 0.25， 0.35 and 0.5 mL， number of colonic mast cells， number of colonic CRFR1- and CRFR2-positive mast cells， expression of hypothalamic CRF， CRFR1， colonic CRF， CRFR1 and CRFR2 proteins （P<0.05，P<0.01）， and a significant decrease in the dwelling time in the center area of OFT， and the expression of hypothalamic CRFR2 （P<0.01）. In contrast to the model group， the AWR scores of 2 Hz-EA group at 0.25 and 0.35 mL and 2 Hz/15 Hz-EA group at 0.25， 0.35 and 0.50 mL were significantly decreased （P<0.05， P<0.01）， and the dwelling time of the three EA groups was obviously increased （P<0.01）. In addition， the increased levels of the number of colonic mast cells， CRFR1- and CRFR2-positive mast cells， and the expression of hypothalamic CRF and CRFR1， colonic CRF， CRFR1 and CRFR2 proteins and the decreased level of hypothalamic CRFR2 protein expression were reversed by 2 Hz-EA， 15 Hz-EA and 2 Hz/15 Hz-EA （P<0.01， P<0.05）. The therapeutic effects of 2 Hz/15 Hz-EA were apparently superior to those of 2 Hz-EA and 15 Hz-EA in reducing the AWR scores， number of colonic mast cells and CRFR1- and CRFR2-positive mast cells， down-regulating the expression levels of CRF， CRFR1 and CRFR2 proteins in colon tissue and CRFR1 proteins in the hypothalamus tissue and in increasing the dwelling time in the center area and the expression of hypothalamic CRFR2 protein （P<0.05）.ConclusionEA of ST36 can improve the general state， visceral sensitivity， anxiety and depression in mice with IBS， which may be related to its functions in lowering the number of CRFR1-and CRFR2-positive mast cells in the colon， down-regulating the expressions of CRF and CRFR1 and up-regulating the expression of CRFR2 protein in the hypothalamus. The effect of 2 Hz/15 Hz-EA is better than that of low frequency-EA and medium frequency-EA.关键词：Electroacupuncture;Irritable bowel syndrome;Visceral sensitivity;Corticotropin releasing factor;Mast cells;Hypothalamus104|239|0更新时间：2026-03-16
- “研究发现电针可改善功能性便秘小鼠的肠道动力及敏感性障碍，其机制可能是通过上调肠嗜铬细胞Piezo通道表达，促进Ca2+内流及5 - 羟色胺分泌。”摘要：ObjectiveTo investigate the effect of electroacupuncture （EA） on intestinal motility and visceral sensitivity in functional constipation （FC） mice and its regulatory mechanism on Piezo1/2 channels in enterochromaffin （EC） cell.MethodsTwenty-four male C57BL/6J mice were randomly divided into normal control， model， and EA groups （n=8 in each group）. The FC model was established by gavage of loperamide hydrochloride. EA （1 mA， 3 Hz/15 Hz） was applied to unilateral “Tianshu” （ST25） and “Shangjuxu” （ST37） for 20 min per session， once daily for 5 consecutive days per week， two weeks altogether. Fecal parameters （number of stool particles in 6 h， characters， and fecal water content）， intestinal motility （the time of the first blue stool excretion after gavage of Evans blue solution and small intestinal transit rate）， and visceral sensitivity （assessed by abdominal withdrawal reflex ［AWR］ in responding to colorectal dilation） were observed. A transmission electron microscopy （TEM） was used to observe EC cells’ ultrastructure. Triple immunofluorescence， Western blot and real-time qPCR were used to detect the expressions of Piezo1/2 in the EC cells and colon， and ELISA was employed to detect the contents of serum and colonic 5-HT and colonic Ca2+.ResultsCompared with the normal group， the model group showed a significant reduction in the number of stool particles， fecal character score， and fecal water content， small intestinal transit rate， AWR scores at 0.25 mL， 0.35 mL and 0.65 mL， contents of serum and colonic 5-HT and colonic Ca2+， expression levels of colonic Piezol1 and Piezol 2 proteins and mRNAs， and the positive areas of Piezol1 and Piezol2， EC， Piezol1/EC and Piezol2/EC （P<0.01， P<0.001，P<0.05）， and an evident increase in the time of the first blue stool particle excretion （decrease of intestinal propulsion） and AWR 3-points threshold （P<0.01）. Following EA treatment， both the decrease and increase of the indexes mentioned above were reversed completely （P<0.05， P<0.001， P<0.01）. TEM showed mitochondrial swelling and a reduction in the secretory granules of EC cells in the model group， which was partially restored following EA treatment.ConclusionEA of ST25 and ST37 can alleviate FC-related intestinal dysmotility and visceral hypersensitivity in FC mice， which may be associated with its functions in up-regulating Piezo1/2 channels of EC cell， promoting Ca2+ influx and 5-HT secretion.关键词：Functional Constipation;Electroacupuncture;Enterochromaffin cell;Piezo;Intestinal motility100|70|0更新时间：2026-03-16
- “专家研究温针灸对膝骨关节炎软骨保护机制，发现其通过调控Hippo-YAP信号通路，促进软骨细胞外基质合成与分解代谢平衡，为治疗膝骨关节炎提供新思路。”摘要：ObjectiveTo observe the effect of warm-needle moxibustion on the synthesis and catabolism of extracellular matrix （ECM） in chondrocytes by regulating the Hippo-YAP signaling pathway in rabbits with knee osteoarthritis （KOA）， so as to explore its mechanisms underlying protection of knee cartilage.MethodsMale New Zealand rabbits were randomly divided into blank control， KOA model， warm-needle moxibustion （moxibustion）， and medication groups， with 7 rabbits in each group. The KOA model was established by using the right hind limb knee joint extension position plaster fixation method. For rabbits of the moxibustion group， warm-needle moxibustion was applied to “Heding”（EX-LE2）， “Neixiyan” （EX-LE4 ） and “Waixiyan” （ST35） for 15 min， once a day， for 14 d. The rabbits of the medication group received gavage of celecoxib （20 mg/kg） once a day for a total of 14 d. The degree of pain， swelling and limitation of movement function of the knee joint were assessed by using Lequesne MG score. The HE staining was used to observe the histopathological changes of knee cartilage tissue. The immunoactivity of Type Ⅱ collagen （COLⅡ） and Aggrecan in the knee cartilage was detected using immunohistochemistry. The expression levels of Yes-associated protein （YAP）， box transcription factor 9 （SOX9）， transforming growth factor-β1（TGF-β1）， matrix metalloproteinase 3 （MMP-3）， and adamalysin-like metalloproteinase with thrombospondin motifs 5 （ADAMTS5） mRNAs and proteins in the knee cartilage tissue were detected by using qPCR and Western blot， separately.ResultsIn contrast to the blank control group， the model group showed a significant increase in the Lequesne MG score， and the mRNA and protein expressions of MMP-3 and ADAMTS5 （P<0.01， P<0.05）， and a considerable decrease in the immunoactivity of COLⅡand Aggrecan， and the mRNA and protein expression levels of YAP， SOX9， TGF-β1 （P<0.01， P<0.05）. After intervention with warm needle-moxibustion and medication， the modeling induced increase and decrease of the levels of the indexes mentioned above were reversed （P<0.01， P<0.05）. No significant differences were found between the moxibustion and medication groups in all the increased and decreased levels of the aforementioned indexes. Observation on changes of the gross morphological structure of the knee joint displayed that the surface of the knee joint in the model group is rough and dark， with obvious congestion， and HE staining showed apparent damage of the ECM， severe disruption of the tidal line and invasion of capillaries into the tidal line in the model group. These injury situations of the knee joint were relatively milder in both the moxibustion and medication groups， including bright joint surface， no obvious congestion in gross morphology， and relatively intact cartilage， and relatively uniform of the ECM， etc.， in HE staining.ConclusionWarm needle-moxibustion can improve the pain reaction， swelling and movement function and protect the cartilage of knee joint in KOA rabbits， which may be related to its functions in regulating Hippo-YAP signaling， promoting the synthesis of cartilage ECM and inhibiting its degradation.关键词：Knee osteoarthritis;Warm needle-moxibustion;Extracellular matrix metabolism;Hippo-YAP signaling pathway;Cartilage protection31|128|0更新时间：2026-03-16
- “专家研究“迎香”穴位注射对AR大鼠的影响，发现其可改善鼻部过敏症状，调节免疫平衡，抑制炎性反应，为AR治疗提供新思路。”摘要：ObjectiveTo observe the effect of “Yingxiang” （LI2） acupoint injection on T-helper 17 （Th17） cell， regulatory T （Treg） cell， and related cytokine expressions in allergic rhinitis （AR） rats， so as to explore its possible mechanism in improving AR.MethodsTwenty-four SD rats were randomly divided into normal， model， and acupoint injection groups （n=8）. The AR model was established using ovalbumin sensitization. The rats of acupoint injection group received injection of a mixture solution of dexamethasone and lidocaine （0.05 mL/acupoint） at bilateral LI2， once every 3 d for a total of 4 times. Nasal allergy symptom scores were recorded； histopathological changes in the nasal mucosa were observed via HE staining； flow cytometry was used to detect the percentage of Th17 and Treg in peripheral blood； the levels of serum allergen-specific immunoglobulin E （sIgE）， interleukin （IL） -10 and IL-17A were detected by ELISA； the mRNA and protein expression levels of forkhead transcription factor p3 （Foxp3） and retinoic acid orphan receptor γt （RORγt） in nasal mucosa were detected by real-time fluorescence quantitative PCR and Western blot， respectively.ResultsFollowing modeling， the symptom scores， Th17/CD4+ T cell ratio in peripheral blood， serum contents of sIgE and IL-17A， and RORγt mRNA and protein expression levels in nasal mucosa were significantly increased （P<0.01）， while the Treg/CD4+ T cell ratio， serum IL-10 content， and Foxp3 mRNA and protein expression levels in nasal mucosa were significantly decreased （P<0.01）. After acupoint injection intervention， the modeling induced increase and decrease of indexes mentioned above were completely reversed （P<0.01）. HE staining revealed thickened nasal mucosa， disordered epithelial arrangement， partial cilia loss， and massive inflammatory cell infiltration in the model group， which were markedly improved in the acupoint injection group.Conclusion“Yingxiang” acupoint injection can effectively alleviate nasal allergy symptoms in AR rats， which may be related to its function in regulating Treg/Th17 immune balance and suppressing inflammatory responses.关键词：Allergic rhinitis;Acupoint injection;Treg/Th17 immune balance;Cytokine;Yingxiang （LI2）35|91|0更新时间：2026-03-16
- “研究显示，针刺治疗能显著改善创伤性脑损伤后吞咽障碍患者的吞咽功能，尤其在咽部运动和感觉功能方面效果显著，还能减少咽喉部分泌物聚集及食物渗漏、误吸和残留。”摘要：ObjectiveTo investigate the clinical therapeutic effect on dysphagia following traumatic brain injury （TBI） treated with acupuncture using fiberoptic endoscopic evaluation of swallowing （FEES）.MethodsA total of 68 patients with dysphagia following TBI were enrolled and randomly divided into a control group and an acupuncture group， with 34 patients in each group. In the control group， the routine medical treatment and swallowing rehabilitation training were given. On the basis of the treatment as the control group， the patients in the acupuncture group received acupuncture at Lianquan （CV23）， Jia-lianquan （Extra）， bilateral Renying （ST23） and Fengchi （GB20）， and Fengfu （GV16）， with needles retained for 30 min in each treatment. The treatment was delivered once daily， with 6 treatments a week， and for a total of 3 weeks in the two groups. The scores of the standard swallowing assessment （SSA） and Kubota’s water swallow test were compared between the two groups before and after treatment. FEES was used to observe the pharyngeal sensory function， pharyngeal motor function， accumulation of secretions， leakage-aspiration and food residue of the two groups.ResultsThe scores of SSA and Kubota’s water swallow test in both groups were reduced in comparison with those before treatment， and the decrease range in the acupuncture group was greater than that in control group （P<0.05）. Pharyngeal sensory function， pharyngeal motor function， the score for accumulation of secretion， leakage-aspiration score and food residue degree were all improved in both groups compared with those before treatment， and the improvements in the acupuncture group were superior to the control group （P<0.05）. The total effective rate in the acupuncture group was 94.12% （32/34）， which was better than that of the control group （79.41%， 27/34， P<0.05）.ConclusionOn the basis of routine medical treatment of internal medicine and rehabilitation training， acupuncture therapy significantly improves swallowing function in patients with dysphagia following TBI， particularly in enhancing pharyngeal motor function and sensory function. Besides， acupuncture effectively reduces the accumulation of pharyngeal secretions， and the severity of food leakage， aspiration， and residue during swallowing.关键词：Dysphagia following traumatic brain injury;Acupuncture therapy;Fiberoptic endoscopic evaluation of swallowing （FEES）;Swallowing function59|80|0更新时间：2026-03-16
- “专家研究针刺中极不同深度治疗女性压力性尿失禁，发现针刺至膀胱前间隙效果最佳，且安全可行。”摘要：ObjectiveTo evaluate the efficacy and safety of acupuncture at Zhongji （CV3） acupoint with different depths for the treatment of female patients with stress urinary incontinence （SUI）.MethodsA total of 90 female patients meeting the inclusion criteria for SUI were randomly divided into treatment group 1， treatment group 2， and control group， with 30 cases in each group. Patients in treatment group 1 received acupuncture at CV3 to the anterior bladder space under CT guidance. Patients in treatment group 2 received acupuncture at CV3 to 10 mm below the abdominal wall. Patients in the control group received acupuncture at CV3 to the abdominal fat layer. All participants had needles retained for 25 min each time， with twice treatments per week for a total of 4 weeks. The patient’s 1-hour urine leakage volume was recorded pre-treatment and after 4 weeks of treatment. The international consultation on incontinence questionnaire-short form （ICI-Q-SF） score was administered at pre-treatment， after 4 weeks of treatment， and follow-up period （8 weeks after treatment completion）. In the end， the efficacy was evaluated based on the 1-hour urine leakage volume. At the same time， the safety and feasibility of clinical treatment were assessed using the acupuncture discomfort visual analog scale（VAS） score， acupuncture tolerance score， and acupuncture safety evaluation.ResultsAfter 4 weeks of treatment， the 1-hour urine leakage volume and the ICI-Q-SF scores were decreased in all 3 groups （P<0.05）. The reductions were greater in treatment group 1 than that in treatment group 2 and the control group （P<0.05）， and greater in treatment group 2 than that in control group （P<0.05）. During the 8-week follow-up period， treatment group 1 and treatment group 2 both showed long-term efficacy （P<0.05）， while the control group did not. The long-term efficacy of treatment group 1 was better than that of treatment group 2 （P<0.05）. The total effective rates were 90.0% （27/30） for treatment group 1 and 86.7% （26/30） for treatment group 2， both higher than control group （16/30， 53.3%，P<0.05）. Meanwhile， both treatment group 1 and treatment group 2 caused no obvious discomfort during treatment and were easy to accept. No adverse events occurred in all patients during the treatment period.ConclusionAll 3 treatment groups can reduce the 1-hour urine leakage volume and the ICIQ-SF scores in female SUI patients to a certain extent， and improve their urinary incontinence symptoms. Among them， acupuncture from the CV3 to the anterior bladder space yielded greater improvement than the other 2 groups and demonstrated better long-term efficacy. Acupuncture from the CV3 to the anterior bladder space and acupuncture from the CV3 to 10 mm below the abdominal wall caused no significant discomfort and were well tolerated， indicating that the regimens are safe and feasible.关键词：Female stress urinary incontinence;Acupuncture;Zhongji （CV3）;Randomized controlled trial26|101|0更新时间：2026-03-16
- “专家研究发现，经皮穴位电刺激（TEAS）辅助麻醉可有效降低腹腔镜胆囊切除术（LC）患者二氧化碳气腹期间心律失常风险，缩短术后苏醒时间，降低苏醒早期疼痛，具有临床应用价值。”摘要：ObjectiveTo investigate the effects of transcutaneous electrical acupoint stimulation （TEAS） as an auxiliary approach to anesthesia on perioperative surface electrocardiogram repolarization indices and emergence quality in patients undergoing laparoscopic cholecystectomy （LC）.MethodsSixty patients undergoing elective LC were randomly assigned to either a TEAS group （30 cases， 2 cases excluded） or a control group （30 cases， 2 cases excluded）. In the TEAS group， the stimulation at bilateral Neiguan （PC6） and Waiguan （TE5） acupoints was initiated before anesthesia， with disperse-dense wave， at the frequency of 2 Hz/10 Hz and the intensity of 4 mA to 10 mA， maintained throughout the surgical procedure. Separately， at the preoperative baseline （t0）， 1 min after anesthesia induction （t1）， 1 min after CO2 pneumoperitoneum establishment （t2）， 30 min after CO2 pneumoperitoneum （t3）， and 10 min after operation （t4）， the electrocardiographic workstation was used to measure the patients’ QT interval dispersion （QTD）， corrected QT interval dispersion （QTcD）， QT interval （QT）， corrected QT interval （QTc）， low-frequency normalized value ［LF（norm）］， high-frequency normalized value ［HF（norm）］， and low-frequency/high-frequency ratio （LF/HF）， as well as the mean arterial pressure （MAP） and heart rate （HR）. Emergence time and visual analog scale （VAS） scores in 1， 6， and 24 h after emergence were recorded， respectively.ResultsCompared with the indicators at t0， QTD was increased in the control group （t2， t3， t4） and the TEAS group （t2） （P<0.001， P<0.01， P<0.05）； and QTcD was increased in the control group （t2， t3， t4） （P<0.001， P<0.01， P<0.05）. In comparison with the control group， QTD and QTcD decreased in the TEAS group （t2， t3， t4） （P<0.001， P<0.05， P<0.01）， HR was reduced in the TEAS group （t2， t3， P<0.05）， and the emergence time was shorter in the TEAS group （P<0.01）， and VAS score was lower in 1 h after emergence （P<0.05）.ConclusionTEAS-assisted anesthesia effectively reduces the risk of arrhythmia during CO2 pneumoperitoneum in patients undergoing LC. Besides， it significantly shortens postoperative emergence time and alleviates pain at early emergence stage， demonstrating a potential clinical value.关键词：Transcutaneous electrical acupoint stimulation;Laparoscopic cholecystectomy;CO2 pneumoperitoneum;QT interval dispersion;Heart rate variability25|57|0更新时间：2026-03-16
- “中国中医科学院广安门医院专家研究发现，术前经皮耳穴电刺激（PAES）可显著提升乳腺癌患者围手术期恢复质量，缓解焦虑，改善睡眠，减轻术后疼痛并减少止痛药使用，且安全性良好。”摘要：ObjectiveTo observe the improvement effect of preoperative percutaneous auricular point electrical stimulation （PAES） on preoperative anxiety state and postoperative quality of recovery in breast cancer patients.MethodsA total of 60 patients who underwent breast-conserving surgery or radical mastectomy for breast cancer in Guang’anmen hospital from August 2023 to August 2024 were enrolled. The patients were randomly divided into a treatment group and a control group. The treatment group received 30 minutes of PAES daily for 3 days before surgery until the day of operation； the control group had electrical stimulation electrodes clamped at the same auricular points without any stimulation. The 15-item Quality of Recovery Score （QoR-15） was used to evaluate patients’ postoperative recovery； the Amsterdam Preoperative Anxiety and Information Scale （APAIS） was applied to assess their preoperative anxiety state； the Pittsburgh Sleep Quality Index （PSQI） was used to evaluate their preoperative sleep quality； the number of non-steroidal anti-inflammatory drug （NSAID） administrations within 48 hours after surgery was statistically analyzed to assess postoperative pain.ResultsThe QoR-15 scores of both groups at 48 hours after surgery were significantly lower than those at admission （P<0.01）， and the QoR-15 score of the treatment group was higher than that of the control group （P<0.01）. Compared with admission， the APAIS anxiety scores of both groups increased before treatment and before surgery （P<0.05）， while the information demand scores before surgery decreased （P<0.05）. The anxiety scores and information demand scores of the treatment group were significantly lower than those of the control group before surgery （P<0.05）. The PSQI scores of both groups before surgery were lower than those before treatment （P<0.05）， and the PSQI score of the treatment group was lower than that of the control group （P<0.05）. After surgery， the number of oral NSAID administrations in the treatment group within 48 hours postoperatively was less than that in the control group （P<0.05）. No PAES （or sham stimulation） related adverse reactions were reported in both groups.ConclusionPreoperative PAES can significantly improve the perioperative quality of recovery in breast cancer patients， alleviate anxiety caused by the disease and surgery， improve sleep quality， relieve postoperative pain and reduce the use of analgesics， with good safety.关键词：Breast cancer;Perioperative period;Anxiety;Percutaneous auricular point electrical stimulation （PAES）;Postoperative quality of recovery20|59|0更新时间：2026-03-16
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Research progress on the mechanism of acupuncture on depression-related brain functional networks AI导读
“专家回顾了针刺调控脑网络治疗抑郁症的研究，从多维度综述其脑功能机制，为该领域研究提供参考。”摘要：Depression is an emotional mental disorder caused by various reasons， characterized by significant and persistent depression. Acupuncture is effective in treating depression， and the relevant mechanism research is also continuing to deepen. Research confirms that abnormal changes in the brain network play a key role in the occurrence and development of depression. This article reviews and sorts out the research on acupuncture regulation of brain network for treatment of depression in recent years. From multiple dimensions such as sensory-related network， cognitive-related network， emotional-related network and brain network interaction， this paper summarizes the brain function mechanism of acupuncture treatment of depression， aiming to provide a useful reference for further research and clinical practice in this field.关键词：Acupuncture;Depression;Brain functional networks71|40|0更新时间：2026-03-16 - “运用数据挖掘技术分析针刺防治围手术期神经认知障碍的临床应用规律，为针刺治疗PND提供参考。”摘要：ObjectiveTo analyze the rules of clinical application of acupuncture in the prevention and treatment of perioperative neurocognitive disorder （PND） by using data mining method.MethodsRandomized controlled trials on acupuncture for preventing and treating PND from PubMed， Embase， Cochrane Library， Web of Science， China National Knowledge Infrastructure （CNKI）， Chinese Biomedical Literature Database （CBM）， VIP Database， and Wanfang Database were retrieved， with the retrieval time ranging from the establishment of each database to July 16， 2024. A relevant database was established to statistically analyze the application of acupuncture methods， selected acupoints， timing of acupuncture intervention， acupuncture stimulation parameters， and stimulation intensity. Additionally， association rules and cluster analysis of acupoints were carried out.ResultsA total of 69 articles were included， with 69 acupuncture prescriptions extracted， involving 40 acupoints. The top 5 acupoints in terms of usage frequency were Neiguan （PC6）， Baihui （GV20）， Zusanli （ST36）， Hegu （LI4）， and Sanyinjiao （SP6）. The top 3 meridians by usage frequency were the Pericardium Meridian of Hand-Jueyin （49 times， 21.68%）， Governor Vessel （44 times， 19.47%）， and Stomach Meridian of Foot-Yangming （30 times， 13.27%）. The top 3 specific acupoints in terms of usage frequency were the crossing acupoints （66 times， 29.20%）， confluence points of the eight vessels （50 times， 22.12%）， and luo-connecting acupoints （49 times， 21.68%）. Regarding acupoints compatibility， the top 3 high-frequency acupoint pairs were PC6-ST36， PC6-GV20， and PC6-LI4. The high-frequency acupoints could be classified into 4 categories： Category 1 includes PC6， ST36， GV20， SP6， and LI4； Category 2 includes Shenmen （HT7） and Yintang （GV24+）； Category 3 includes Benshen （GB13）， Taichong （LR3）， Sishencong （EX-HN1）， and Shenting （GV24）； Category 4 is Fengchi （GB20）. The top 2 acupuncture treatment methods by usage frequency and times were transcutaneous electrical acupoint stimulation （TEAS） and electroacupuncture （EA）. The intervention timing was mostly selected during and before surgery. For EA stimulation， sparse-dense waves were commonly used， with a frequency of 2 Hz/100 Hz， adjusted to the patient’s tolerance.ConclusionPC6， GV20， ST36， LI4 and SP6 are the core acupoints for the prevention and treatment of PND， and TEAS or EA is usually performed during or before the operation.关键词：Acupuncture;Perioperative neurocognitive disorder;Data mining;Rules of acupoint selection;Timing of intervention15|4|0更新时间：2026-03-16
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