前の章|次の章

Application of virus vector tools for acupuncture analgesia related brain circuits research

  • role： First author第一作者
  • Affiliation：

    College of Acupuncture-Moxibustion and Massage， Gansu University of Chinese Medicine， Lanzhou 730101， China

MA Cui,  
  • Affiliation：

    College of Acupuncture-Moxibustion and Massage， Gansu University of Chinese Medicine， Lanzhou 730101， China

YE Yu-juan,  
  • role： Corresponding author通信作者
  • Affiliation：

    College of Acupuncture-Moxibustion and Massage， Gansu University of Chinese Medicine， Lanzhou 730101， China

  • Email：yanxingke@126.com
  • Introduction：严兴科，E-mail： yanxingke@126.com
YAN Xing-ke*

サマリー

The clinical efficacy of acupuncture analgesia is definite， but its mechanisms of the central nervous system have not yet been fully elucidated. The virus vector technique provides an effective means for the study of the central nervous system mechanism of acupuncture analgesia. We， in this article， briefly introduced the classification and characteristics of virus vectors， reviewed and analyzed their application and their functional components carried by and optical/chemical genetics technologies they carry in pain and acupuncture analgesia research in recent years. At present， in the research on the central mechanism of acupuncture analgesia， the viral vectors combined with chemical genetics technology is mainly used to explore the neural circuit mechanisms such as rostral anterior cingulate cortex （rACC）→ventrolateral periaqueductal gray （rACC→vlPAG）， rACC→thalamus （rACC→Th） and ventrolateral periaqueductal gray→hippocampus （vlPAG→HPC）. The viral vectors combined with optogenetics technology is mainly used to explore the neural circuits from the prefrontal infralimbic cortex to the nucleus accumbens （IL→NAc）. The viral vectors and optical/chemical genetics technology can not only target and accurately mark the specific neurons or neural circuits， but also dynamically and real-time monitor the function of neural networks， bringing new experimental means and breakthroughs for the research of acupuncture analgesia mechanism of the brain circuits.

キーワード

Virus vector tool;Acupuncture analgesia;Optogenetics;Chemical genetics;Brain circuits

References

  1. 1.
    XU Y. Comparative study on the efficiency of adenovirus vector and lentivirus vector in gene editing chickens （in Chinese）［D］. Nanning： Guangxi University， 2020.
  2. 2.
    JIN S. Establishment of tool virus system and its application in neural loop analysis （in Chinese）［D］. Beijing： University of Chinese Academy of Sciences， 2016.
  3. 3.
    HAN Z P， SHI X W， YING M， et al. Tools for neural circuit tracing based on neurotropic viruses （in Chinese）［J］. Chinese Journal of Analytical Chemistry， 2019， 47（10）： 1639-1650.
  4. 4.
    CHEN Y Z. Optogenetics （in Chinese）［J］. Science， 2014， 66（4）： 21-26.
  5. 5.
    LI X Y， ZHU Y C， SUN H J， et al. Electroacupuncture inhibits pain memory and related anxiety-like behaviors by blockading the GABAB receptor function in the midcingulate cortex［J］. Mol Neurobiol， 2023， 60（11）： 6613-6626.
  6. 6.
    SU H， CHEN H Y， ZHANG X， et al. Electroacupuncture ameliorates pain in cervical spondylotic radiculopathy rat by inhibiting the CaMKII/CREB/BDNF signaling pathway and regulating spinal synaptic plasticity［J］. Brain Behav， 2023， 13（10）： e3177.
  7. 7.
    LI B， ZHU L， ZHOU Y C， et al. Studies on neuronal tracing with pseudorabies virus （in Chinese）［J］. Chinese Journal of Virology， 2014， 30（3）： 333-337.
  8. 8.
    ZHANG G R， ZHAO H， ABDUL-MUNEER P M， et al. Neurons can be labeled with unique hues by helper virus-free HSV-1 vectors expressing Brainbow［J］. J Neurosci Methods， 2015， 240： 77-88.
  9. 9.
    ZHANG Z J， JIN S， ZHU X T， et al. Advancement in neurotropic virus-mediated trans-synaptic neural circuit tracing （in Chinese）［J］. Chinese Bulletin of Life Sciences， 2014， 26（6）： 634-644.
  10. 10.
    WICKERSHAM I R， SULLIVAN H A， SEUNG H S. Axonal and subcellular labelling using modified rabies viral vectors［J］. Nat Commun， 2013， 4： 2332.
  11. 11.
    HAVRANEK K E， REYES BALLISTA J M， HINES K M， et al. Untargeted lipidomics of vesicular stomatitis virus-infected cells and viral particles［J］. Viruses， 2021， 14（1）： 3.
  12. 12.
    OSAKADA F， MORI T， CETIN A H， et al. New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits［J］. Neuron， 2011， 71（4）： 617-631.
  13. 13.
    BLESSING D， DÉGLON N. Adeno-associated virus and lentivirus vectors： a refined toolkit for the central nervous system［J］. Curr Opin Virol， 2016， 21： 61-66.
  14. 14.
    ZHENG C X， WANG S M， BAI Y H， et al. Lentiviral vectors and adeno-associated virus vectors： useful tools for gene transfer in pain research［J］. Anat Rec， 2018， 301（5）： 825-836.
  15. 15.
    LIU Y H， CUI J J， LI Y Q， et al. Application prospect of conditional gene editing animals and viral vectors in experimental research of acupuncturology （in Chinese）［J］. Acupuncture Research， 2023， 48（3）： 299-304.
  16. 16.
    BERNDT A， LEE S Y， WIETEK J， et al. Structural foundations of optogenetics： Determinants of channelrhodopsin ion selectivity［J］. Proc Natl Acad Sci U S A， 2016， 113（4）： 822-829.
  17. 17.
    BOYDEN E S. Optogenetics and the future of neuroscience［J］. Nat Neurosci， 2015， 18（9）： 1200-1201.
  18. 18.
    MAZZITELLI M， MARSHALL K， PHAM A， et al. Optogenetic manipulations of amygdala neurons modulate spinal nociceptive processing and behavior under normal conditions and in an arthritis pain model［J］. Front Pharmacol， 2021， 12： 668337.
  19. 19.
    SMITH M L， ASADA N， MALENKA R C. Anterior cingulate inputs to nucleus accumbens control the social transfer of pain and analgesia［J］. Science， 2021， 371（6525）： 153-159.
  20. 20.
    GAN Z， GANGADHARAN V， LIU S， et al. Layer-specific pain relief pathways originating from primary motor cortex［J］. Science， 2022， 378（6626）： 1336-1343.
  21. 21.
    MENG Q. Study on the regulation of pain and anxiety-like behavior by neural circuits in somatosensory cortex （in Chinese）［D］. Hefei： University of Science and Technology of China， 2019.
  22. 22.
    SINGH A， PATEL D， LI A N， et al. Mapping cortical integration of sensory and affective pain pathways［J］. Curr Biol， 2020， 30（9）： 1703-1715.
  23. 23.
    MEDA K S， PATEL T， BRAZ J M， et al. Microcircuit mechanisms through which mediodorsal thalamic input to anterior cingulate cortex exacerbates pain-related aversion［J］. Neuron， 2019， 102（5）： 944-959.
  24. 24.
    LEE M， MANDERS T R， EBERLE S E， et al. Activation of corticostriatal circuitry relieves chronic neuropathic pain［J］. J Neurosci， 2015， 35（13）： 5247-5259.
  25. 25.
    ZHANG H X， QIAN Y L， LI C， et al. Brain-derived neurotrophic factor in the mesolimbic reward circuitry mediates nociception in chronic neuropathic pain［J］. Biol Psychiatry， 2017， 82（8）： 608-618.
  26. 26.
    CAI Y Q， WANG W， PAULUCCI-HOLTHAUZEN A， et al. Brain circuits mediating opposing effects on emotion and pain［J］. J Neurosci， 2018， 38（28）： 6340-6349.
  27. 27.
    JOHNSON A C， LATORRE R， LIGON C O， et al. Visceral hypersensitivity induced by optogenetic activation of the amygdala in conscious rats［J］. Am J Physiol Gastrointest Liver Physiol， 2018， 314（3）： G448-G457.
  28. 28.
    SONG J W， PATEL R V， SHARIF M， et al. Chemogenetics as a neuromodulatory approach to treating neuropsychiatric diseases and disorders［J］. Mol Ther， 2022， 30（3）： 990-1005.
  29. 29.
    BENBADIS S R， GELLER E， RYVLIN P， et al. Putting it all together： Options for intractable epilepsy： an updated algorithm on the use of epilepsy surgery and neurostimulation［J］. Epilepsy Behav， 2018， 88S： 33-38.
  30. 30.
    YAN X， YANG X F. Exploration and prospects of chemogenetics in treatment of epilepsy （in Chinese）［J］. Journal of Clinical Neurosurgery， 2021， 18（2）： 229-232.
  31. 31.
    CHEN D D， ZHOU Y， ZHAI X J， et al. Recent advances of brain circuit dissection in animal models of pain based on optogenetics and chemogenetics （in Chinese）［J］. Chinese Journal of Pain Medicine， 2022， 28（1）： 7-20.
  32. 32.
    YIN J B， LIANG S H， LI F， et al. dmPFC-vlPAG projection neurons contribute to pain threshold maintenance and antianxiety behaviors［J］. J Clin Invest， 2020， 130（12）： 6555-6570.
  33. 33.
    LIANG H Y， CHEN Z J， XIAO H， et al. nNOS-expressing neurons in the vmPFC transform pPVT-derived chronic pain signals into anxiety behaviors［J］. Nat Commun， 2020， 11（1）： 2501.
  34. 34.
    ALHADEFF A L， SU Z W， HERNANDEZ E， et al. A neural circuit for the suppression of pain by a competing need state［J］. Cell， 2018， 173（1）： 140-152.
  35. 35.
    JIANG Y Y， SHAO S， ZHANG Y， et al. Neural pathways in medial septal cholinergic modulation of chronic pain： distinct contribution of the anterior cingulate cortex and ventral hippocampus［J］. Pain， 2018， 159（8）： 1550-1561.
  36. 36.
    XIA S H， HU S W， GE D G， et al. Chronic pain impairs memory formation via disruption of neurogenesis mediated by mesohippocampal brain-derived neurotrophic factor signaling［J］. Biol Psychiatry， 2020， 88（8）： 597-610.
  37. 37.
    HIRSCHBERG S， LI Y， RANDALL A， et al. Functional dichotomy in spinal- vs prefrontal-projecting locus coeruleus modules splits descending noradrenergic analgesia from ascending aversion and anxiety in rats［J］. eLife， 2017， 6： e29808.
  38. 38.
    GAO S H， SHEN L L， WEN H Z， et al. The projections from the anterior cingulate cortex to the nucleus accumbens and ventral tegmental area contribute to neuropathic pain-evoked aversion in rats［J］. Neurobiol Dis， 2020， 140： 104862.
  39. 39.
    ZHU X X， XU Y L， SHEN Z， et al. Rostral anterior cingulate cortex-ventrolateral periaqueductal gray circuit underlies electroacupuncture to alleviate hyperalgesia but not anxiety-like behaviors in mice with spared nerve injury［J］. Front Neurosci， 2021， 15： 757628.
  40. 40.
    SHEN Z， ZHANG H Y， WU Z M， et al. Electroacupuncture alleviates chronic pain-induced anxiety disorders by regulating the rACC-thalamus circuitry［J］. Front Neurosci， 2020， 14： 615395.
  41. 41.
    ZHU H， XIANG H C， LI H P， et al. Inhibition of GABAergic neurons and excitation of glutamatergic neurons in the ventrolateral periaqueductal gray participate in electroacupuncture analgesia mediated by cannabinoid receptor［J］. Front Neurosci， 2019， 13： 484.
  42. 42.
    LIU H. The role of prefrontal cortical-nucleus accumbens neural circuit in relieving the reward effect of acupuncture on chronic pain （in Chinese）［D］. Shanghai： Shanghai University of Traditional Chinese Medicine， 2020.
  43. 43.
    WANG J， CUI J J， HA L J， et al. Review on application of neural tracing technique to experimental research of acupuncture （in Chinese）［J］. Acupuncture Research， 2019， 44（12）： 926-932.
  44. 44.
    LIU R. Study on the loop mechanism of lateral parabrachial nucleus in neuropathic pain and anxiety （in Chinese）［D］. Hangzhou： Zhejiang University， 2021.
  45. 45.
    TAO S J. Analysis of hippocampal neural structure circuits based on tool virus system optimization （in Chinese）［D］. Wuhan： Huazhong University of Science and Technology， 2022.
  46. 46.
    LI B， YANG X Y， QIAN F P， et al. A novel analgesic approach to optogenetically and specifically inhibit pain transmission using TRPV1 promoter［J］. Brain Res， 2015， 1609： 12-20.
  47. 47.
    ZHANG Y， WANG J Y， LI S Y， et al. Application prospect of common viral tracers in study on brain effect of acupuncture （in Chinese）［J］. Chinese Acupuncture & Moxibustion， 2021， 41（2）： 205-209.
  48. 48.
    KIM S， KIM N， LEE J S， et al. Dynamic Fas signaling network regulates neural stem cell proliferation and memory enhancement［J］. Sci Adv， 2020， 6（17）： eaaz9691.
  49. 49.
    HILGER D， MASUREEL M， KOBILKA B K. Structure and dynamics of GPCR signaling complexes［J］. Nat Struct Mol Biol， 2018， 25（1）： 4-12.
  50. 50.
    MIYASHITA T， SHAO Y R， CHUNG J， et al. Long-term channelrhodopsin-2 （ChR2） expression can induce abnormal axonal morphology and targeting in cerebral cortex［J］. Front Neural Circuits， 2013， 7： 8.
  51. 51.
    JIANG J W， CUI H X， RAHMOUNI K. Optogenetics and pharmacogenetics： principles and applications［J］. Am J Physiol Regul Integr Comp Physiol， 2017， 313（6）： R633-R645.
  52. 52.
    MANVICH D F， WEBSTER K A， FOSTER S L， et al. The DREADD agonist clozapine N-oxide （CNO） is reverse-metabolized to clozapine and produces clozapine-like interoceptive stimulus effects in rats and mice［J］. Sci Rep， 2018， 8（1）： 3840.
  53. 53.
    JENDRYKA M， PALCHAUDHURI M， URSU D， et al. Pharmacokinetic and pharmacodynamic actions of clozapine-N-oxide， clozapine， and compound 21 in DREADD-based chemogenetics in mice［J］. Sci Rep， 2019， 9（1）： 4522.
  54. 54.
    HE Q Y， SHEN Z， SHE L J， et al. Feasibility of joint application of techniques of optogenetics and neuroelectrophysiology to research of acupuncture analgesia （in Chinese）［J］. Acupuncture Research， 2018， 43（8）： 476-479， 491.

続きを読む

The above content is generated by Large Model Translation. The translated content is for reference only. We do not assume any commercial or legal responsibilty for any consequences arising from the use of our website