我们曾经报告

脑内乙酰胆碱和5-羟色胺在针刺镇痛过程中都具有重要作用。应用密胆碱(HC-3)阻断脑内乙酰胆碱合成

可以明显降低针效;若在此基础上补充胆碱

又可使针效恢复。应用对氯苯丙氨酸(pCPA)阻断脑内5-羟色胺的合成

针效亦明显降低;相反

应用5-羟色氨The role played by central 5-hydroxytryptamine(5-HT)and acetylcholine(ACh)in Acupunc-ture Analgesia(AA)has been studied in rats.Blocking of synthesis of ACh by intraventricular injection of hemicholine(HC-3)reduced theeffect of AA following the lowering of the 5-HT and 5-HIAA content in the central nervoussystem.The blocking effect of HC-3 on AA could be reversed by choline

the precursor of ACh

followingthe increasing of central 5-HT and 5-HIAA.The blocking effect of HC-3 could also be reversed by the injection of 5-Hydroxytryptophan(5-HTP)to increase the content of central 5-HTFrom the results stated above

it follows that central ACh and 5-HT seem to be the importantfactors mediating the acupuncture analgesia

but the effect of ACh on AA might possibly bemediated through the effect of central 5-HT.