血循环中去甲肾上腺素(NA)的水平可用以反映外周交感神经的活动情况

不同脑区组织内去甲肾上腺素含量可以反映中枢神经系统去甲肾上腺素能神经的机能状态

但都需要一种微量的分析方法

为此

我们改建了去甲肾上腺素的放射酶测定法。在去甲肾上腺素的测定中

经典的生物测定法虽然敏感

但去甲肾上腺素和肾上腺A simple

sensitive and specific radioenzymetic assay for the measurement of noradrenaline has been improved.This assay is based on the noradrenaline transforming into

3

H-adrenaline(3H-adrenaline(

3

H-AD)in the presence of phenylethanolamine-N-methyl transferase(PNMT)and 3H-AD)in the presence of phenylethanolamine-N-methyl transferase(PNMT)and

3

H-sadenosyl-methionine(3H-sadenosyl-methionine(

3

H-SAME).PNMT was extracted from fresh bovine adrenals.3H-AD was counted by a liquid scin-tillation counter.The sensitivity of this method is about 50pg.It is applicable in the measurementof NA kor many body tissues and fluids

including brain and plasma.