在我们以往的工作中

已经观察到;对黄牛进行电针镇痛时

脑脊液内乙酰胆碱含量增加

停针后可持续30分钟以上。为了在临床上进一步证明针麻对外科手术病人脑脊液中乙酰胆碱含量的影响

探讨乙酰胆碱在针刺镇痛中的作用。我们选择神经外科采用针麻手术的病人

观察针麻手术中的不同阶段脑脊液内乙酰胆碱的含量变化。实验方法对13例针麻神经外科手术病人在手术前、后不同阶段脑脊液内乙酰胆碱的含量进行了测定。其中男9例

女4例。息颅脑内13 neurosurgical patients operated under acupuncture anesthesia were selected

and the contents of Ach in cerebrospinal fluid were tested at various intervals during operation. It was found: (1) After induction of acupuncture

incision of skin and incision of brain tissue

the quantity of Ach was apparently higher than that before needling

and there was a considerable significance through analysis of variance. (p<0.01). (2) To compare the Ach content in cerebrospinal fluid during opera- tion with that after induction of acupuncture

the concentration of Ach was decreased gradually with the lapse of time

but without statistical significance. (3) It is indicated that the analgesic effect of acupuncture anesthesia appears to be correlated intimately with the elevation of the Ach level in cerebrospinal fluid of the patients.