国内许多资料认为神经系统是针感反应的物质基础

也有认为针感的产生与血管有一定的关系。我们用儿茶酚胺荧光和胆碱酯酶组织化学方法观察了皮下组织中小血管周旁大量神经分布

对它们做了定性和相互关系的研究

曾提出过用这些方法所反映出的神经和经络实质

针刺原理的关系。国内外都有资料强调针刺针麻效应都产生于穴位深部组织

多在肢体肌肉内。因此观察骨骼肌

关节囊等深部组织神经分布就很必要

我们特别重视了与血管有关的神经。材料和方法The innervation of rat skeletal muscle

profound fascia

intermuscular connective tissue and joint capsules were studied by means of histochemi- cal techniques for the separate and consecutive demonstration of choline- sterase(ChE)and catecholamine fluorescence. Both the adrenergic or cholinergic nerves surrounding the resistance vessels of these tissues. All these vessels were showed dual innervation. It has been known that sympathetic cholinergic system innervated the arteries of skeletal muscle only. In our experiment of stretched preparation of skeletal muscle and subcutaneous or profound fascia

the sympathetic cholinergic nerve termi nals were also accompanied with the same arteriole supplying both skele- tal muscle and subcutaneous or profound fascia. As iso-OMPA was not added in ChE incubated medium

ChE-positive nerves and their axonal terminals

i. e. free nerve endings

were ob- served in skeletal muscle

profound fascia

intermuscular connective tis- sues and joint capsule. A lot of free nerve endings are present in these tissues

especially in skeletal muscle. The significance of the presence nature of these free nerve endings in the afferent nerve conduction of muscle was discussed.