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中医研究院针灸所神经解剖组
纸质出版日期:1983
移动端阅览
李瑞午, 陶之理. 胃副交感内脏运动节前纤维的发端——HRP法研究[J]. 针刺研究, 1983,(3):182-189.
Li Ruiwn, TaoZhili. THE ORIGINAL NUCLEI OF THE PARASYMPATHETIC PREGANGLIA FIBERS OF STOMACH (H R P METHOD)[J]. Acupuncture research, 1983, (3): 182-189.
支配胃的副交感节前纤维是迷走神经的一部分。关于迷走神经内脏运动节前纤维的起源问题一般均认为是起源于迷走神经运动背核。这是Marinesco首先提出的。以后经Molhant
Husten
Getz
Bell等人用核外染色质溶解的方法证实了。但目前对这一问题仍有很多争论
主要认为这些学者们所采用的研究方法本身是并不十分Up to now
the problem of the origin of the parasympathetic pregan- glia fibers of stomach is still at issue. Somebody considered that the pre- ganglia fibers of visceral motor of vagal nerve originated in the nodal ganglion; some considered that the dorsal motor nuclei of vagus did not innervate the visceral smooth muscle
and some suggested that stomach was innervated doubly by the dorsal motor nuclei of vagus and the medialis nuclei of tractus solitarii. Thus
We think that it is necessary to further study the origin of the parasympathetic preganglia fibers of the stomach. 24 rabbits were used in this study. 80-100ul of 10% HRP
dissolved in 0.9% saline
were injected into the tunica muscularis of the stomach of rabbits. Among them
6 were injected in the anterior wall of stomach. 6 in the posterior wall
6 in the cardiac pars and 6 in the pyloric pars. After a survival period of 5 days the animals were intracardially perfused with 2% paraformaldehyde in 0.1M phosphate buffer (PH7.2-7.4). The medulla were removed and cut serially at 40μm on a freezing microtome. The sections were divided into two parts and treated with 3'
3
diamino- benziue (D.A.B) and benzindine hydrochloride (B.D.H.C) separately. Sections were conterstained with neutral red. The HRP labelled cells were found within the dorsal motor nuclei of vagus in the present study. The projective area of stomach is situated in the supra-middle part of these nucle
close to medialis
the marked level of obex from above the obex 2-3 mm to below the obex 1-2mm
totally about 4-5mm cephalocaudally. most of these cells
nearly 93% of the total labelled cells
are small ones. The four different parts of the stomach
anterior wall
posterior wall
cardiac and pyloric pars
do not occupy specific location within the dorsal motor nuclei of vagus. In the present study the HRP labelled cells were only found within the dorsal motor nuclei of vagus
but the HRP labelled cells were not seen in the medialis nuclei of tractus solitarii. This study shows that the dorsal motor nuclei of vagus join to adjust the functional activity of the stomach Our previous study showed the connection of the dorsal motor nuclei of vagus with the sensory fibers of trigeminal nerve
Therefore
the inhibition of the stretch reaction of stomach is completed by the dorsal motor nuclei of vagus when the facial point are accurpunctured.
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