我们以往工作证明了针刺对蓝斑、缝际核、视上核及弓状核内乙酰胆硷酯酶有非常明显的激活作用。为了进一步了解针刺作用原理

我们企图进一步研究针刺对下丘脑中这些重要核团内与蛋白质、类脂类代谢以及膜渗透性调节有关的非特异性酯酶的作用

从而阐明针刺作用机制。方法本实验选用健康成年雄性大白鼠34只

随机分成针刺组(22只)、对照组(12只)。测表1 针刺对大白鼠痛阈的影响Thirty four healthy

adult and male rats were selected for this experi- ments

These animals were divided at random into acupuncture and the con- trol groups. The pain threshold were measured before decapitation. The cryostat sections were made for nonspecific esterase (NsEase) reaction and measurement with microphotometer. α- naphthol acetate and fast blue B salt was used to demonstrate NsEase in the brain section. 10

(-1)

10(-1)

10

(-5)

M of E 600 was used as inhibitors of this enzyme. The results are as follows. 1) The influence of acupuncture upon supraoptic nucleus (SON) : The number of NsEase positive neurons and their reaction in SON of the acupuncture group were increpsed significantly than that of the con- trol. 2) The influence of acupuncture upon the ependyma of Ⅲ ventricle: At the level of media eminentia

The reactien of NsEase of acupuncture group was increased to significant level

as compared with the control. 3) The influence of acupuncture on NsEase of arcuate nucleus (Arc N): The reaction product of NsEase in the neurons of Arc N was faint brown yellow in color. There was no significant difference found between the acupuncture group and the control. This experiment indicated that acupuncture could activiate the NsEa- se of SON and ependyma of the Ⅲ ventricle. The mechnism of their actions and relations with other histochemical components under acupuncture anaesthesia were discussed in this paper.