肥大细胞为神经激素细胞

其颗粒中含有多种生物活性物质

在针刺中将起着重要的作用。本实验采用形态学方法

观察“足三里”穴区肥大细胞的分布和当针刺时肥大细胞的变化

为揭示肥大细胞在针刺过程中起何种作用提供形态为基础。方法本实验采用正常健康雄性大白鼠17只。平均体重为380克。观察两侧“足三里”穴共计34个穴区。实验分为两组:(1)对照组:采用正常大鼠

在1％戊巴比妥钠轻度麻醉下

为了保持穴区皮肤组织不受移动和牵拉

经断头后

取下两侧后肢

固定24小In revealing the function of mast cell for providing morphological bases under acupuucture

seventeen normal male rate were used. Totally 34 areas of both "Zusanli" points were observed

acupunctured with manupu- lation. Normal rats under slight ansthesia with 1% sodium barbiturate for keeping the point area of the skin not to be moved and pilled. Both legs cut out were after decapitation and fixed in Schaffer's solution for 24hr

then both sides of "Zusanli" areas were excised

and fixed once again and whole skin spreading specimens and wax section preparations were made. The slight ansthesia was applied to the acupunctured group as well as the control. The both side of "Zusanli" were punctured with needles keeping in the depth of the muscle layer

twirling slightly for 5 minutes

then the preparations were made that as of the control

then stained with toluidine blue. The results were observed as follows: 1. Normal distribution of mast cells in point "Zusanli"

area: There were round

oval

long and irregular formed mast cells aggregated or single distributed in skin and subcutaneous tissues of the whole skin spreading specimens. Some of them were arranged along the small blood vessels in lines

especially along the subcutaneous longitudinal and transversal bra- nches and multiple branches from the superficical to deep. There were dispersed and aggregated immature and matured mast cells around the hair follicles and outer membranes of small vessels and fat cells and in the connective tissues. After continual sectional observation

The mast cells in muscle tissue were accompanid with the small blood vessels and their bran- ches passed through perimysium into connective tissues of muscle bundle and distributed dispersively or around the small blood vessels. It demon- strated that the mast cells in muscle were accmpanied with vessels. 2. Acupuncture group. The single particle was released in the sub- cutaneous tissues around small vessels

muscle bundle and connective tis- sues within muscular fibres

in sections of the preparations

above resulte indicated that acupuncture could elicit the release and lysis of particles in the mast cells around the point. Mast cell is a kind of neurohormoral cell

containing 5-HT

histami- ne

hydrolase and other biological active substances. Acupuncture could clicit the release of these particles and in which histamimine could enha- nce the vaso-permeabilities

the plasma protain could oozed out from the vasa

which activates the phagocytosis of phagocytes to phagositize the damaged tissues by acupuncture. Owing to the acupuncture elicites the damage of acupoints points. We consider that the phagocytosis of the phagocytes might purify the necro- tic tissues of acupoints points. So that the physiologic equilibrium of acupoints oints is remained.