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中国中医研究院针灸研究所
纸质出版日期:1986
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文琛, 赵长龙. 针刺“人中”对失血性休克家兔肝磷酸化酶作用的组织化学研究[J]. 针刺研究, 1986,(4):258-262.
THE EFFECT OF ACUPUNCTURE AT "RENZHONG" ON HEPATIC PHOSPHORYLASE OF RABBIT DURING HEMORRHAGIC SHOCK HISTOCHEMICAL OBSERVATION[J]. Acupuncture research, 1986, (4): 258-262.
大失血后高血糖是由于肝细胞糖原分解释放葡萄糖。我们已观察到针刺“人中”可对失血性休克家兔肝糖原有显著的影响
因为磷酸化酶是糖原分解的限速酶
以组化方法显示出磷酸化酶的活性
可进一步了解针刺“人中”对失血性休克家兔肝细胞功能的影响。材料和方法雄性家兔104只
右股动脉放血
失血量为20毫升/公斤
血压下降到50毫米汞柱以下
失血后10分钟(7例)、30分钟(13例)心电监视和血压同时都出现濒危者(19例)
平均为67分钟取材。针刺104 male rabbits were used in this experiment. The groups divided and the order of physiological experiment was just the same as that in "The effect of acupuncture at "Renzhong" on hepatic glycogen of rabbits during hemorrhagic shock". Phosphorylase is a rate-limiting enzyme for glycogenolysis. The straight chain unbranched polysacchride associated with phosphorylase
stains bluc- black with iodine
distributed mainly at periportal liver cells under nor- mal condition
while the branched polysaccharide stains red brown distri- buted at perivenous zone of hepatic lobule. The phosphorylase was was activted largely in hepatic lobule and de- monstrated the glycogenolysis is taking place 10 min. after bleeding. The phosphorylase of hepatic cells disappeared and depleted gradually 30 min. After bleeding. The phosphorylase was decreased more quickly among these three hemorrhagic groups and there was a statistic significance. The phosphorylase of hepatic cells in acupunctured group was activted continually 30 min. after bleeding
it was distributed at the hepatic cell membrane bordering on sinusoids and then decreased. but a greater extent than that without acupuncture. There was a statistic singificance between the acupuncture groups and non-acupuncture groups. It is benificial to sup- ply various tissues and orgens depleted out of hemorrhage with energy from glycogenolysis.
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