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中国中医研究院针灸研究所
纸质出版日期:1986
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文琛, 赵长龙. 家兔肝实质的交感神经分布及其在失血性休克时的针刺效应[J]. 针刺研究, 1986,(4):252-257.
SYMPATHETIC INNERVATION OF RABBIT LIVER PARENCHYMA AND THEIR EFFECT OF ACUPUNCTURE DURING HEMORRHAGIC SHOCK[J]. Acupuncture research, 1986, (4): 252-257.
失血性休克后
肝糖原大量分解形成高血糖
而交感神经及其有关神经递质——儿茶酚胺(CA)是对肝释放葡萄糖的重要因素。因此了解在失血性休克时肝的交感神经递质的变化是一个重要方面。由于对实验室常用动物肝实质内是否存在交感肾上腺素能神经还有争论
我们在针刺“人中”对失血性休克家兔肝组化的研究中
以诱发CA荧光的方法观察了家兔肝实质的肾上腺素能神经分布及其在失血性休克时的针刺效应。
53 healthy male rabbits were divided into four groups. They were di- vided into acupuncture at "Renzhong" point group and nonacupuncture group after hemorrhagic shock.The liver tissues were excised 10 or 30 min. after bleeding of the rabbits of non-acupuncture. The imminent state about which were mean time 67 min.
the hepatic blocks were removed. The excised time of animals liver of acupuncture groups was just the same to non-acupuncture one
but they were sure survival. In addition there were normal control group and CCl_4 injected group. Cryostat sections were made and catecholamine fluorescence induced was with glyoxylic acid The sympathetic innervation of adrenergic fibers could be observed in nor- mal rabbit liver parencyma. Fluorescent varicose nerve terminaks ran bordering sinusoids and in close contact to both hepatocytes and endothe- lial cells of sinusoids. The varicose nerve terminals displayed green fluo- rescence
the characteristic for catecholamines. The adrenergic nerve fibers in peripheral zone of hepatic lobule were more dense than others. The density of adrenergic nerve in different lobuli was not same. There were an abundance of sympathetic adrenergic nerves in portal area and surroun- ding of the the vessels wall formed groud plexus network. It was discu- ssed that the sympathetic nerve might control the liver blood flow and regulate hepatocytic metabolism. After hemorrhagic shock
the adrenergic terminals in the liver tissues were decreased markedly
especially excised from rabbit liver in imminent state. It has not been decreased apparently and the adrenergic varicose terminals to normal level nearly by acupuncture at "Renzhong" point. It is benificial to control the liver blood flow and hepatocytedmeabolism by sympathetic nerve fibers.
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