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中国中医研究院针灸研究所
纸质出版日期:1986
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文琛, 赵长龙, 刘金兰, 等. 针刺“人中”对失血性休克家兔肝糖原影响的组织化学研究[J]. 针刺研究, 1986,(4):263-267.
THE EFFECT OF ACUPUNCTURE AT "REN" ZHONG" ON HEPATIC GLYCOGEN OF RABBITS DURING HEMORRHAGIC SHOCK--A HISTOCHEMICAI STUDY-[J]. Acupuncture research, 1986, (4): 263-267.
很早就知道失血后血中葡萄糖显著增加是因失血后肝细胞经过糖原分解释放葡萄糖的缘故。以组化方法观察失血性休克发生后肝糖原的变化以及针刺“人中”对失血性休克动物肝糖原的影响是探讨针刺作用原理的一个环节。材料和方法雄性家兔103只
体重2.1~3公斤
分为9个组:失血性休克3个组
从右股动脉放血
血压降至50毫米汞柱以下
平均失血为20毫升/公斤
人工呼吸
并有心电监护
于失血后10、30分钟以及动物濒危时(平均67分钟)取材。针刺组也分为3个103 male rabbits were divided into nine groups. The blood pressure was fall down to below 50mm Hg after bleeding 20ml/kg
the hepatic blocks were excised in 10 and 30 min. and the animals were in imminent dangerous state (mean 67 min.). The animals of acupuncture at "Ren zhong" were divided into three groups: 30 min.
mean 67 min. and 120 min. after bleeding. Normal
sham operation and CCl_4 injection were used as control. The histochemical changes of hepatic glycogen has been observed and measured quantitatively with Leitz MPV_2 compact microscope photome- ter. The liver glycogen from periportal to perivenous zone of hepatic lo- bule was decreased
disappeared and depleted gradually after hemorrhage. The hepatic glycogen was decreased more quickly among these three hemor- rhagic groups and there was a statistic significance. The hepatic glycogen in three acupunctured groups were also decreased ane disappeared
but much more better in time and quantity than that without acupuncture. The relation between these phenomena and their acupuncture effect was dis- cussed in this paper.
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