Kong Tianhan, Fan Tiansheng, Han Xuefei, et al. RESEARCH ON SEROTONERGIC NEURONS IN LATERAL RAPHE NUCLEUS OF RAT WITH INTRACELLULAR INJECTION HRP TECHNIQUE[J]. Acupuncture research, 1992, (3): 196-200.
Kong Tianhan, Fan Tiansheng, Han Xuefei, et al. RESEARCH ON SEROTONERGIC NEURONS IN LATERAL RAPHE NUCLEUS OF RAT WITH INTRACELLULAR INJECTION HRP TECHNIQUE[J]. Acupuncture research, 1992, (3): 196-200.DOI:
大鼠中缝背核5-HT能神经元树突的形态学观察——细胞内HRP注射之研究
摘要
中缝背核(dorsal raphe nucleus
DR)头端平动眼神经核尾极
尾端达背侧被盖核水平
其背面紧邻第四脑室底头端及中脑导水管尾端腹面
在滑车神经核的水平
DR可进一步分为背正中亚核、腹内侧亚核This experiment was carried out on 35 rats with stereotaxic apparatus following a new coordinate which was designed by ourselves. The microelectrode was pushed into the lateral dorsal raphe nucleus(LDR)under direct vision. The neurons with regular rhythm and slow firing rate were searched by intracellular recording
then the singular cell in LDR was filled with HRP by intracellular iontophoresis. Results: 7 HRP labelling neurons with serotoninergic firing characteristics were observed in LDR. The labelling dendrites were found in neighboured structure(periaqueductal gray
laterodorsal tegmental nucleus
dorsal tegmental nucleus
4th ventricle). This work demonstrated that LDR plays some role in acupuncture analgesia.