Thirty rabbits

weighing 2. 5 - 3.0kg

were divided into three groups of 10 rabbits each. 10% HRP 50 - 80μl was injected into "Guan Yuan Shu" point and "Pang Guang Shu" point and 10% HRP 100 - 150μl injected into the urinary bladder. The animals were survived for 3 - 4 days

using 1% paraformaldehyde solution in phosphate buffer to perfuse their hearts

then take the thoracic ganglia

lombar ganglia and their spinal cord

which were made frozen sections of 40μm and TMB method reaction. The segmental distribution of afferent neurons of point and urinary bladder was observed under the light microscope.The segmental distribution of afferent neurons of the "GuanYuan Shu" point was L1-S5

the "Pang Guang Shu" Point was L2 - S5

urinary bladder was T12 - L1 - CO1. These afferent nerve fibers proected into Ⅲ- Ⅳ lamina.The experimental studies show that the afferent neurons of "Guan Yuan Shu"

"Pang Guang Shu"POint and urinary bladder are mutual intersection and overlap in the spinal ganglia of L2 - S5

extending to 9 segments. These afferent fibers converge to the Ⅲ- Ⅳ lamina in spinal cord.The mutual intersection and convergence of these fibers may be the morphological foundation of regulating the function of urinary bladder with acupuncture at "Guan Yuan Shu"

"Pang Guang Shu" point.The results may elucidate the mechanism of correlation between acupoint and viscera too.