we used the method of in situ hybridization to explore the PPE-mRNA expression of the myenteric neurons after electroacupuncture. Results showed that the expression of PPE-mRNA after electroacupuncture were much more than those of the control. It suggests that there may be a close relationship between an increasing of PPE-mRNA expression in the enteric nervous system induced by electroacupuncture and the regulation of acupuncture on the function of gastrointestinal tract.