In order to research the relation between PVN and the effects of needling "Shenshu"

we studied c-fos expressions in PVN of rats induced by electroacupuncture and acupuncture at the right "Shenshu" using immunohistochemistry. The results show that densities of FLN in PVN are significantly differ- ent (P < 0. 01

all)

the electroacupuncture group > the acupuncture group > the control group > the normal group; the stimuli side of electroacupuncture group > the non-stimuli side of electroacupuncture group >the non-stimuli side of acupuncture group > the stimuli side of acupuncture group. The results indicate that PVN takes part in the effecting process of needling "Shenshu" and the degree of PVN taking pat in the effecting change depends on different ways and amount of stimuli. It is also indicated that the difference showed by the experiments may be one important link of different effects induced by different needling method and different effects between two sides induced by needling on one side.