本实验采用高脂饲料喂养SD雄性大鼠制造肥胖动物模型

观察针刺对其体重、体脂和纹状体组织单胺类神经介质含量的影响。结果显示

肥胖大鼠纹状体酪氨酸 (Tyr)、多巴胺 (DA)、去甲肾上腺素 (NA)、色氨酸 (Trp)、N+

K+ ATP酶和Ca2 + ATP酶均明显低于正常水平

而 5 羟色胺 (5 HT)和 5 HT/5 羟吲哚乙酸 (5 HIAA)明显高于正常水平。针刺治疗在取得良好减肥效应的同时

肥胖大鼠纹状体Tyr、NA、DA、Trp、Ca2 + ATP酶水平明显回升

而 5 HT和 5 HT/5 HIAA水平明显回降。提示针刺对肥胖大鼠纹状体的良性作用可能是其实现减肥效应的中枢作用机制之一Objective: To investigate the mechanism of acupuncture in treatment of simple obesity. Methods: 33 rats were randomly and evenly divided into normal group

obesity control group and obesity + acupuncture group. Obesity model was established by feeding the rats with high fat diet. Bilateral “Zusanli" (ST 36) and “Neiting” (ST 44) were needled and stimulated with electrical pulses (10 Hz

1.5 V

10 min)

once daily continuously for 12 days. At the end of the experiments

the fat in the pericardium

groin and around the kidney were separated and weighted. Contents of tyrosine (Tyr)

dopamine (DA)

noradrenaline (NA)

tryptophan (Trp)

5 hydroxytryptamine (5 HT) and 5 hydroxyindoleacetic acid (5 HIAA)in the striatum tissue homogenate were detected with fluorospectrophotometry; the Na +

K + ATPase and Ca 2+ ATPase activity was determined with zymochemical method. Results: Tyr

DA

NA and Trp contents in obesity rats were lowered while 5 HT content and 5 HT/5 HIAA higher than those of normal group. After acupuncture treatment

the rat body weight was decreased

Tyr

NA

DA

Trp

Ca 2+ ATPase levels raised significantly (P<0.05

P<0.01); while 5 HT and 5 HT/5 HIAA reduced markedly (P<0.05). Conclusion: Acupuncture has a good action on reducing body weight via regulating monoamine neurotransmitter levels and raising ATPase activity in the striatum.