应用酶联免疫吸附实验 (ELISA)法测定缺血大鼠电针后脑内脑源性神经营养因子(BDNF)含量的变化。实验分正常组、缺血再灌组和缺血再灌 +电针组

大脑中动脉阻塞 (MCAo)致局灶缺血 90min

缺血后立即给予电针

取“水沟”和“百会”穴

强度 3.5mA

频率 1 0 0Hz

时间1hr。再灌 8hr后取缺血侧皮质

ELISA法测定。结果正常组、缺血再灌组和缺血再灌 +电针组脑内BDNF含量分别为 1 4.2 0± 3.1 4、1 8.75± 2 .6 3和 2 3.75± 3.0 2 (ng/ g)

缺血 +电针组BDNF含量多于缺血组 (P <0 .0 5 )。表明电针能提高缺血后脑内BDNF的合成或释放Objective: To observe the effect of electroacupuncture (EA) on brain derived neurotrophic factor(BDNF) content of the cerebral cortex in rats with cerebral ischemia. Methods: 18 Wistar rats were randomly and evenly divided into normal control

ischemia and EA+ischemia groups. The middle cerebral artery was occluded for 90 min to induce focal ischemia. After cerebral ischemia

EA (3.5 mA

100 Hz) was applied to “Shuigou” (GV 26) and “Baihui” (GV 20) for one hour. After 8 hr reperfusion

the ischemic cortex was sampled and the BDNF content determined with enzyme linked immunosorbent assay (ELISA). Results: It was found that BDNF contents in the infarction region of the cerebral cortex in control

EA and EA ischemia groups were 14.20±3.14

18.75±2.63 and 23.75 ±3.02 ng/g respectively. Conclusion: EA could increase the synthesis and release of BDNF in the brain after cerebral ischemia and reperfusion.