Objective:To observe the therapeutic effect of electroacupuncture (EA) on T lymphocyte subgroups of peripheral blood in the rat.Methods: Forty Sprague Dawley rats were divided into five groups: control group

“Zusanli”(ST 36) group

immunosuppressive model group

none point group and immunosuppressive model+ EA group by blocked randomization. Immunosuppressive model was established by intraperitoneal injection of cyclophosphamide 100 mg/kg (twice). Bilateral “Zusanli” (ST 36) were punctured and stimulated with frequency 2 Hz and 3 V for 30 min

once daily

continuously for 7 days. Rats of the immunosuppressive and control group were only fixed to an animal stand for 30 min at the same hours every day. Blood samples were collected on the 7th day after intraperitoneal injection of pentobarbital sodium (1%

30 mg/kg). The T lymphocyte subgroups of peripheral blood (including CD + 4 and CD + 8) were measured by direct microvolume whole blood immunoflurescent staining technique with flow cytometer(FCM) to evaluate the immune function of rats from different aspects. Results: ① The value of CD + 4 in EA group was significantly higher than that of control group(P<0.01)

and the values of CD + 8 between the two groups had no statistical difference

indicating that EA had a markedly regulative effect on the immune function.② The value of CD + 4 in immunosuppressive model group was obviously lower than that of control group(P<0.01)and the values of CD + 8 between the two groups had not any statistical difference (P>0.05)

indicating that cyclophosphamidum(CY)restrained the T lymphocyte subgroups of peripheral blood.③ The value of CD + 4 in the immunosuppressive model + EA group was significantly higher than that of immunosuppressive model group(P<0.01).④ The data of the none point group were not different from those of control group(P>0.05). Conclusion: EA on “Zusanli” point can improve cellular immune induced by T lymphocytes

but EA on none point has no such function.