本实验采用透射电镜技术对家兔急性心肌缺血后心肌缺血区肌原纤维、线粒体及血小板结构的变化及针刺的作用进行了观察。结果 :①缺血组肌原纤维的明暗带变浅或消失

Z带呈屈曲状 ;线粒体积聚、肿胀

有的线粒体嵴断裂和血小板脱颗粒发生。②电针“内关”穴后

心肌的横纹和肌原纤维的明暗带清晰可见

多数线粒体未见肿胀和积聚成堆现象发生

血小板也没有出现脱颗粒。结果提示

针刺“内关”穴可保护心肌细胞免于在急性心肌缺血期间受损Objective: To observe the protective effect of electroacupuncture (EA) of “Neiguan” (PC 6) on ischemic cardiac myofibrilla

mitochondria and blood platelets in the rabbit. Methods: 12 mongrel rabbits were randomly and evenly divided into control group

ischemia group and ischemia+EA group. Bilateral “Neiguan” (PC 6) were punctured and stimulated with electrical pulses (3 V

40 Hz and intermittent waves) for 30 min. Myocardial ischemia was produced by occlusion of the left ventricular branch of the coronary artery. After sectioning

the myocardial samples taken from the ischemic marginal region were observed by using a transmission electron microscope. Results: ① In ischemia group

both the dark and light bands of some myofibrilla became lighter in color or disappeared; Z bands presented tortuous like changes; mitochondria got aggregate and swollen. Some mitochondrial cristae were broken and degranulation of platelets appeared; ② In EA+ischemia group

both the dark and light bands of the myofibrilla could be seen clearly

the Z band was normal

no swelling and no aggregation of majority of the mitochondria and no degranulation of platelets were found. Conclusion: EA of “Neiguan” (PC 6) can protect the ischemic cardiac myocytes from injury.