目的 :探讨原癌基因c fos在穴位电针对胃运动影响中的表达及其意义。方法 :采用免疫组织化学方法及电生理的方法

观察电针刺激“足三里”等不同穴位

c fos在中枢延髓的孤束核(NTS)及迷走神经背运动核 (DMV)中的表达

同时采用浆膜法检测胃电变化情况。结果 :电针刺激“足三里”等不同穴位c fos在NTS及DMV中的表达情况不同

且胃电也发生较为明显的变化。结论 :穴位电针对胃运动具有调节作用。以c fos的表达作为激活标志

提示这种调节作用可能是通过对NTS及DMV神经元的激活而实现的Objective:To observe c fos gene expression and analyze its significance in electroacupuncture (EA) regulation on gastric motor in rats. Methods:64 SD rats were randomly divided into "Zusnali" (ST 36) group (n=8)

"Shangjuxu" (ST 37) group (n=8)

non acupoint group (n=8) and control group (n=8). By using immunohistochemical technique (ABC method)

c fos gene expression in the Dorsal motor nucleus of the vagus nerve (DMV) and nucleus tractus solitary(NTS) was displayed. After anesthesia (846 mixture

0.1 mL/100 g body weight)

the rat's abdominal cavity was opened and an induction electrode was implanted at the serous coat of the anterior wall of the stomach (about 0.3 ～0.5 cm to the pylorus) for recording EGG(electrogastrography). Left "Zusanli"(ST 36) and "Shangjuxu"(ST 37) were stimulated with EA by setting the stimulating parameters being frequency of 50 Hz

voltage of 20 V and duration of 30 min. Results:In control group

c fos positive neurons in DMV and NTS were few. After EA of "Zusanli"(ST 36) and "Shangjuxu"(ST 37)

c fos positive neurons increased considerably in number in comparison with those of control group (P<0.01). While those of "Zusanli"group were obviously more than those of "Shangjuxu" group (P<0.01) but no significant difference was found between non acupoint group and control group (P>0.05). Simultaneously

changes (increase of both frequency and amplitude after EA) of EGG of the four groups presented the same tendency. Conclusion: EA can adjust gastrtic motor function

which may be closely associated with EA activating neurons of NTS and DMV.