目的 :探讨腧穴不同组方对急性胃粘膜损伤大鼠胃粘膜形态学的影响。方法 :以乙醇造成大鼠急性胃粘膜损伤后

动态观察针刺“足三里”不同组穴对模型胃粘膜组织学及超微结构的影响。结果 :①“足三里”不同穴组对急性胃粘膜损伤均具有保护作用。②在不同穴组中

以“足三里” +“内关” +“中脘”组效果最好。结论 :“足三里” +“内关” +“中脘”可考虑做为临床针灸治疗胃病的基本处方Objective: To observe the effect of different prescriptions of acupoints on morphological changes in rats with experimental acute injury of gastric mucosa(AIGM). Methods: 72 Wistar rats anesthetized with pentobarbital (40～50 mg/kg) were randomized into control group (n=6)

model group

"Zusanli" group

"Zusanli"+"Neiguan" group

"Zusanli" +"Zhongwan" group and "Zusanli" +"Neiguan" +"Zhongwan" group. The last 5 groups were further divided into immediate subgroups

gastric samples were taken immediately after electroacupuncture (EA) treatment and 8 hr-subgroups (samples were taken 8 hours after EA)

with 6 cases being in each subgroup. Acute gastric injury model was established by intragastric perfusion of absolute ethyl alcohol (1 mL). Bilateral "Zusanli" (ST 36)

bilateral "Neiguan" (PC 6) and "Zhongwan" (CV 12) were punctured and stimulated electrically with frequency 30 Hz

10～20 V and duration of 10 min. At the end of each experiment

the rat was killed for taking out the whole stomach that was embedded with paraffin and then sectioned and stained with HE. The sections of the stomach were observed under light microscope and electronic microscope for analyzing changes of the injured gastric mucosa. Results: ① After administration of alcohol

gastric mucosal injury was apparent in every subgroups compared with control group

but the injury degrees in every acupuncture subgroup were significantly lighter than that of model subgroups (P<0.05～0.001). ② Among the different acupoint prescription groups

the injured severity of "Zusanli" +"Neiguan" +"Zhongwan" group was lightest under both light microscope and electronic microscope (no obvious swelling of the mitochondria

regular arrangement of the mitochondria crest and the endoplasmic reticulum without dilation). Conclusion: Acupuncture treatment has a protective action on alcohol induced injury of the gastric mucosa

and the prescription of "Zusanli" +"Neiguan" +"Zhongwan" may be used as the basic prescription for treating gastric diseases in clinic.