目的 :研究针刺“内关”穴对心肌梗塞后血管生成相关因子bFGF和TGF β1 的干预作用。方法 :采用免疫组化方法检测心肌bFGF和TGF β1 。结果 :结扎冠脉 2hr后即可见到心肌细胞内有bFGF和TGF β1 表达

2天时bFGF最少 ;而TGF β1 却增至最多。 1周后bFGF较 2天增多

开始在血管周围分布

3周时少量bFGF分布于尚未形成完整的血管上 ;而TGF β1 却较 2天时减少

3周时少量TGF β1 分布于细胞内。二者之间的变化表现出“拮抗”关系。针刺组bFGF表达较同一时相的模型组增多

并且血管生成较快

较完整 ;而TGF β1 针刺组却较同一时相的模型组减少。针刺“内关”对bFGF的生成具有促进作用

而对TGF β1 的产生却具有抑制作用。结论 :针刺“内关”穴具有调节缺血心肌血管生成相关因子bFGF和TGF β1 生成的作用Objective: To research the effect of acupuncture of "Neiguan" (PC 6) on bFGF and TGF-β 1 (angiogenic related factors) expression in ischemic myocardium in the rat. Methods: 72 Wistar rats were randomized into normal group

sham-operation group

model group

and acupuncture group. The later 2 groups were further divided into 2 hr

2-day

1-week

2-week and 3-week subgroups

with 6 cases in each subgroups and the former two groups. Myocardial ischemia (MI) model was established by occlusion of the anterior descending branch of the left coronary artery. Bilateral "Neiguan" (PC 6) were punctured and stimulated with handle manipulation (1 min) and electroacupuncture apparatus (WQ-10CZ

40 Hz

positive pulse amplitude 4 V and duration of 5 min). bFGF and TGF-β 1 expression was shown by staining the ischemic myocardium sections by immunohistochemical method and observed under light microscope. Results: The bFGF and TGF-β 1 were not expressed until 2 hours after coronary vessel was ligated. 2 days after MI bFGF expression level was the least

while TGF-β 1 expression peaked. One week after MI

bFGF expression level increased compared with that of 2-day subgroup

and distributed around the capillary vessels

myocardial cellular membrane and cellular plasma. Three weeks after MI

lighter TGF-β 1 expression was found and distributed in myocardial cellular

cellular plasma and the vascular wall

which reduced compared with that at 2 days

while lighter bFGF expression distributed in the blood vessels that had not become matured . The variation between bFGF and TGF-β 1 expression showed an antagonistic effect. In acupuncture subgroups

bFGF expression was much heavier than that of the model group at various same phases

furthermore the formation of blood vessels of acupuncture group was more rapidly and entirely than that of model groups. The TGF-β 1 expression in acupuncture subgroups was opposite to bFGF expression. It displayed that acupuncture of "Neiguan" (PC 6) could promote generation of bFGF and had an inhibitory effect on TGF-β 1. Conclusion: Acupuncture of "Neiguan" (PC 6) may promote formation of newborn blood vessels via modulating expression of angiogenic related factors bFGF and TGF-β 1 in the ischemic myocardium.