目的 :观察艾灸治疗实验性豚鼠哮喘病中NO(一氧化氮 )与M受体的相互关系。方法 :采用 50只 2 50～ 3 0 0 g的雄性豚鼠

随机分成 :A 健康对照组

B 实验哮喘组

C 哮喘 +艾灸组

D 哮喘 +L NAME组

E 哮喘 +L NAME(L 精氨酸甲酯 ) +艾灸组共五组

用放射性配基结合实验的方法测定肺组织细胞膜M受体Bmax和平衡离解常数

用NO检测试剂盒测各组豚鼠BALF(支气管肺泡灌洗液 )、肺组织、血中NO的含量。结果 :与健康组相比

哮喘组豚鼠BALF中NO含量升高

肺细胞膜制剂对3H QNB结合的Bmax上升 ;与哮喘组相比

哮喘 +艾灸组及哮喘 +L NAME +艾灸组豚鼠BALF中NO含量下降

肺细胞膜制剂对3H QNB结合的Bmax降低

哮喘 +L NAME组豚鼠BALF中NO含量降低

而肺细胞膜制剂对3H QNB结合的Bmax无明显变化。结论 :艾灸“肺俞”等穴可降低哮喘引起的肺组织和肺泡灌洗液中NO水平及细胞膜Bmax的增加

NO与Bmax在灸法缓解哮喘过程中呈正相关关系Objective:To investigate the role and correlation of nitric oxide (NO) and M receptor in moxibustion treatment of asthma in guinea pigs. Methods: 50 guinea pigs were randomly and evenly divided into five groups: ① control group

②asthma group

③ moxibustion group

④ L NAME (non selective inhibitor of NO) group

and ⑤L NAME + moxibustion group. Asthma model was established by intraperitoneal injection of 10% egg albumin saline (1 mL) and inhaling 1% aerosol egg albumin in guinea pigs. The 3H QNB(quinuclidinyl benzilate) combined Bmax and equilibrium dissociation constant (Kd) of M receptor on the lung tissue cell membrane were calculated using Scatchard formula according to the detected radioactivity value with radioaligand binding assay

and NO levels of plasma

bronchoalveolar lavage fluid(BALF)and lung tissues were assayed according to instructions of the reagent kit. Bilateral "Feishu" (BL 13)

"Dazhui" (GV 14) and "Dingchuan" (EX B 1) were moxibustioned for 20 min.Results: Results indicated that Bmax level of 3H QNB combined M receptor of asthma group and asthma+L NAME group increased significantly in comparison with those of control group (P<0.01); there were no significant differences between moxibustion and control groups

and between moxibustion+L NAME and control groups

suggesting an inhibitory effect of moxibustion on Bmax. In spite of being higher of kd value in moxibustion group

no significant differences were found among the 5 groups (P>0.05). Regarding NO content changes

there were no significant differences among the 5 groups in plasma NO; while BALF and lung tissue NO contents of asthma group increased considerably in comparison with the other 4 groups (P<0.01)

but no significant differences were found between moxibustion

or L NAME

or moxibustion+L NAME groups and control group

indicating a down regulative action of moxibustion on BALF and lung tissue NO levels. In asthma model group

moxibustion group and moxibustion+L NAME group

a positive correlation between nitric oxide level and Bmax of M receptor exists(γ=0.732

0.718

0.716

P<0.05)

and L NAME had no obvious affection on the Bmax of M receptor.Conclusion:Moxibustion can suppress asthma induced increase of NO contents in the lung tissues and BALF and Bmax level; and there exists a positive correlation between NO level and Bmax of M receptor.