Objective: To observe the influence of acupuncture combined with medicine on the content of calmodulin(CaM) activity in rat ischemic cerebral region. Methods: The animal models of the cerebral ischemia with reperfusion were made by electric coagulation of bilateral vertebral arteries and occlusion of the ipsilateral common carotid artery. A total of 45 Wistar rats were randomly divided into five groups: ① normal control group (n=9)

② model control group(n=9)

③ sham operation group (n=9)

④ electroacupuncture group[EA of"Shangyang"(LI 1)

"Zhongchong"(PC 9)

"Lidui" (ST 45) and "Zuqiaoyin"(GB 44)

2～20 Hz

20 min

[WT5BX]n=9]

⑤ EA + medicine group (Venoruton 90 mg/200 g body weight was given by intragastric perfusion an hour before the model was made

⑤ EA + medicine group (Venoruton 90 mg/200 g body weight was given by intragastric perfusion an hour before the model was made

n=9). CaM activity of the ischmic cerebral tissues was measured by using phosphodiesterase method. Results:When cerebral ischemia occurred

CaM activity of the ischemic cerebral region elevated obviously in comparison with normal control group and sham operation group (P<0.05～0.001). Following administration of EA and EA + medicine

active CaM contents were significantly lower than that of model group (P<0.001). There was no evident difference between EA and EA + medicine groups in CaM contents (P>0.05). Conclusion: Both EA and EA+ medicine can reduce ischemia reperfusion induced increase of active CaM level in the rat.