目的 :观察电针“水沟”与“井穴”对全脑缺血大鼠脑细胞内活性钙调素 (CaM )含量的影响。方法 :采用大鼠全脑缺血再灌注模型

用磷酸二脂酶法测定电针后正常对照组、模型对照组、电针“水沟”穴组、电针“井穴”组、假手术对照组脑组织活性CaM的含量。结果 :脑缺血后脑组织活性CaM含量明显升高

电针刺激“水沟”和“井穴”均可使大鼠缺血区脑组织活性CaM含量明显降低

两组无显著性差异。结论 :电针“水沟”与“井穴”均能明显降低活性CaM的含量

发挥肯定的脑保护作用Objective: To observe the influence of electroacupuncture(EA) at "Shuigou" (GV 26) and "Jing acupoints" respectively on the content of calmodulin(CaM) activity in rat ischemic cerebral region. Methods: The animal models of the cerebral ischemic with reperfusion were made by electric coagulation of bilateral vertebral arteries and occlusion of the ipsilateral common carotid artery. A total of 45 Wistar rats were randomly divided into five groups: ① normal control group(n=9)

② model control group(n=9)

③ sham-operation group(n=9)

④ EA of "Shuigou" group(2～20 Hz

20 min

n=9)

⑤ EA of "Jing acupoints" group["Shangyang" (LI 1)

"Zhongchong"(PC 9)

"Lidui" (ST 45)

"Zuqiaoyin" (GB 44)

2～20 Hz

20 min

n=9]. CaM activity of the ischemic cerebral tissues was measured by using phosphodiesterase method. Results: In model group

CaM activity of the ischemic cerebral region elevated obviously in comparison with normal control group and sham-operation group (P<0.001).Following administration of EA

active CaM contents were significantly lower than that of model group (P<0.001). There was no evident difference between EA of "Shuigou" group and EA of "Jing acupoints" group in CaM contents(P>0.05). But CaM contents in both EA groups were still significantly higher than those of normal control group and sham-operation group(P<0.001

P<0.01). Conclusion: Both EA of "Shuigou" and "Jing acupoints" can reduce ischemia-reperfusion induced increase of active CaM level in the rat.