目的 :探讨电针双侧“合谷”穴区对Wistar大鼠局灶脑缺血 /再灌注时大脑皮质cPKCα蛋白表达的影响。方法 :线栓法闭塞Wistar大鼠大脑中动脉

制备局灶脑缺血 /再灌注模型。运用免疫组化法检测电针大鼠双侧“合谷”穴区对局灶脑缺血 /再灌注时大脑皮质cPKCα蛋白表达的影响。结果 :假手术组部分动物大脑皮质cPKCα蛋白弱表达

局灶脑缺血 3hr时大脑皮质cPKCα蛋白表达增加

但未达显著水平 (P >0 .0 5)

再灌注 3hr、6hr时大脑皮质cPKCα蛋白表达明显增加(P <0 .0 1 )

而电针可以明显减少再灌注 3hr、6hr时大脑皮质cPKCα蛋白表达 (P <0 .0 1 )。结论 :电针“合谷”穴区可下调大脑皮质cPKCα蛋白表达

这可能与电针抗局灶脑缺血 /再灌注时脑细胞凋亡有关Objective: To explore the effect of electroacupuncture (EA) of "Hegu" (LI 4) on cPKCα protein expression in cerebral cortex in ischemia-reperfusion Wistar rats. Methods: 42 Wistar rats were randomly and evenly divided into sham-operation (control) group

ischemia(I)-3 hr group

I-3 hr+reperfusion (R)-3 hr group

I-3 hr+R-6 hr group and I-3 hr+EA group

I-3 hr-R-3 hr+EA group

and I-3 hr-R-6 hr+EA group (with 6 rats being in each group). Rat Middle cerebral artery occlusion and reperfusion model was established by intraluminal nylon filament-ball occlusion and withdrawal. Cytoplasm cPKCα protein expression in cerebral cortex was displayed using immunohistochemical staining method. EA (acupuncture + electrical stimulation

40～60 Hz

1.5 V and duration of 30 min) was applied to bilateral "Hegu"point(LI 4). Results: In control group

very weak cPKCα protein expression was found in some cerebral cortex and basal ganglion neurons in only 3 rats. In I-3 hr group

cPKCα protein expression increased slightly(P>0.05). While in I-3 hr-R-3 hr group and I-3 hr-R- 6 hr group

cPKCα protein expression increased markedly (P<0.01). Following EA stimulation

cPKCα protein expression in I-3 hr-R-3 hr and I-3 hr-R-6 hr groups reduced significantly in comparison with I-3 hr-R-3 hr and I-3 hr-R-6 hr groups (P<0.01)

showing an inhibitory action of EA on cPKCα protein expression. Conclusion: EA of bilateral "Hegu"could reduce cerebral ischemia-reperfusion generated cPKCα protein expression

which may be responsible for acupuncture's antiapoptotic mechanism.