目的 :探讨戊四唑 (PTZ)点燃型癫痫大鼠脑内氨基酸的变化及电针对其影响。方法 :成年SD大鼠 3 0只随机分为三组 (每组 1 0只 ) :癫痫模型组、电针组、正常对照组。采用氨基酸自动分析仪测定脑内氨基酸含量。结果 :癫痫模型组脑内兴奋性氨基酸谷氨酸 (Glu)及抑制性氨基酸甘氨酸 (Gly)升高明显 (P <0 .0 5) ;而抑制性氨基酸γ 氨基丁酸 (GABA)降低明显 (P <0 .0 1 )。经电针治疗后γ 氨基丁酸 (GABA)、丙氨酸 (Ala)含量明显升高 (P <0 .0 1、P <0 .0 5)

而Glu降低明显 (P <0 .0 5)。结论 :电针抗癫痫的作用可能与脑内Glu、GABA及Ala的变化有关Objective: To observe the changes of amino acids in the brain of pentylenetetrazole(PTZ) kindled epilepsy rats and to explore the effect of electroacupuncture (EA). Methods: 30 adult SD rats were randomly divided into three groups (10 rats in each group): control group

epilepsy model group and EA group. Epilepsy rat model was established by intraperitoneal injection of PTZ (32 mg/kg)

once daily

continuously for 25 days. EA treatment was given to the rat by puncturing and electrically stimulating "Baihui" (GV 20) and "Fengchi" (GB 20) for 30 min (100 Hz

6 mA)

once daily

continuously for 7 days. Contents of aspartic acid (ASP)

glutamic acid (Glu)

glycine (Gly)

γ-aminobutyric acid (GABA) and alanine (Ala) in the brain tissue supernatant were measured with 835-50 Type Amino-acid Automatic Analysis Apparatus. Results: In epilepsy model group

ASP

Glu and Gly contents increased

while GABA and Ala levels decreased in comparison with those of control group. Among them

the increase of Glu (excitatory amino acid) and Gly (inhibitory amino acid glycine) and the decrease of GABA were remarkable (P<0.05～0.01). After EA treatment

the contents of ASP

Glu and Gly presented a decreasing tendency

while those of GABA and Ala increased considerably compared with model group(P<0.01

P<0.05)

and the decrease of Glu level was significant (P<0.05).Results indicated that EA could lessen the contents of excitatory amino acids and raise those of some inhibitory amino acids in the brain tissue of epilepsy rats. Conclusion: The effect of EA in treatment of epilepsy is likely related with EA generated changes of excitatory and inhibitory amino acids levels in the brain tissues.