目的 :进一步探索针刺对钙调素活性的影响

研究钙调素在经络活动、针刺效应中的作用。方法 :在前期有关研究的基础上

采用磷酸二酯酶法 (PDE)测定针刺“下脘”穴后

“下脘”穴局部及其同经的“巨阙”穴处的钙调素 (CaM)活性的变化。结果 :针刺“下脘”穴后

其局部钙调素的活性明显高于同经“巨阙”穴的活性 (P <0 0 1 )

且两穴的活性明显高于针刺前任脉组织的活性 (P<0 0 1 )。结论 :针刺可能对钙调素有一定的激活作用 ;针刺对钙调素的激活作用可能沿着经络系统传递Objective : To study the influence of acupuncture on the activity of calmodulin(CaM) and to analyze the effects of CaM in the activities of meridians and the effect of acupuncture. Methods: Forty Wistar rats were divided into "Xiawan" group (n=10)

"Juque" group (n=10) and Conception-Vessel group (n=20). Under conscious state

the rat was fixed on an experimental board

"Xiawan" (RN 10) was punctured and stimulated with uniform reinforcing-reducing method at a frequency of 80 times/min

continuously for 1 min

and the acupuncture needle was retained for 5 min. Under ether-anesthesia

the rat's abdominal tissues along the Conception Vessel from "Shuifen" (RN 9) to "Juque" (RN 14) were taken before acupuncture

and the tissues around "Xiawan" (RN 10) and "Juque" (RN 14) collected after acupuncture for determining the activity of CaM using phosphodiesterase(PDE) technique. Results: Following acupuncture of "Xiawan" (RN 10)

the activity of CaM of "Xiawan" (RN 10) area is obviously higher than that of "Juque" (RN 14) (P<0.01); and the activity of CaM in "Xiawan" (RN 10) and "Juque" (RN 14) are all higher than that of tissues along Conception Vessel (P<0.01). Conclusion: Acupuncture may activate the calmodulin(CaM)

which is probably involved in the signal transmission of acupuncture along the meridians.