目的 :观察“十二井穴”针法对局灶性脑缺血大鼠TGF β1的时效性影响。方法 :采用光化学诱导Wistar大鼠一侧大脑造成局灶性脑缺血模型

施以“十二井穴”针法在造模后 1、3、6、1 2、2 4hr不同时间段开始进行针刺治疗

治疗 6天后处死大鼠

沿缺血中心制成冠状面切片

运用免疫组化染色观察各治疗组半暗带与对照组神经细胞TGF β1表达量的变化

并进行图像分析。结果 :各时段模型组TGF β1表达的总面积及 3、6、1 2、2 4hr各组的积分光度均比假手术及正常组明显升高 (P <0 0 5)

针刺组 3、6、1 2、2 4hrTGF β1表达的总面积及 6、1 2、2 4hrTGF β1表达的积分光度均较同时段模型组明显升高 (P <0 0 5或P <0 0 1 )。结论 :针刺对保护半暗带神经元具有重要意义Objective: To observe changes of transforming growth factor (TGF)-β1 expression in neurons of the brain after cerebral ischemia and acupuncture of the "twelve Jing points" in the rats. Methods: 96 Wistar rats were randomized into model group (n=30)

acupuncture group (n=30)

sham-operation group (n=30) and control group (n=6)

the former 3 groups were further evenly divided into 1

3

6

12 and 24 hr subgroups. The rat focal cerebral ischemia model was made by photochemical method to block blood flow of the middle cerebral artery. The "twelve Jing points"(LU-11

LI-1

PC-9

TE-1

HT-9

SI-1) were punctured with filiform needles

starting respectively at 1

3

6

12

24 hr after establishing ischemia models

once daily

6 times altogether. 6 days later

the rats were put to death for taking the brain tissue sample (ischemic region) that was then cut into coronal slices. Immuno-histochemical staining was performed for displaying changes of TGF-β1 expression of the cerebral neurons in the penumbra region. Then image analysis was conducted. Results: In comparison with sham-operation and control groups

TGF-β1 expression area of model group increased significantly (P<0. 01) from 3 hr on after cerebral ischemia. Compared with model group

TGF-β1 expression area of acupuncture group increased further significantly from 3 hr on after cerebral ischemia (P< 0.05

0.01). The integral luminosity value of various groups presented a similar tendency in spite of appearing later (6 hr after cerebral ischemia) in the significant difference. Morphological observation indicated that TGF-β1 expression came from microgliocytes and phagocytes. Conclusion: The results prove that acupuncture can significantly upregulate TGF-β1 expression

which maybe contribute to the protection effect of acupuncture on ischemic cerebral neurons.